Claims
- 1. An isolated cDNA comprising a nucleic acid sequence encoding a protein selected from the amino acid sequence of SEQ ID NO:1 and SEQ ID NO:2, or the complement thereof.
- 2. A purified protein or a portion thereof selected from:
a) an amino acid sequence of SEQ ID NO:1 and SEQ ID NO:2; b) an antigenic epitope of SEQ ID NO:1 or SEQ ID NO:2; and c) a biologically active portion of SEQ ID NO:1 or SEQ ID NO:2.
- 3. A composition comprising the protein of claim 2 and a pharmaceutical carrier.
- 4. A method for using a protein to screen a plurality of molecules or compounds to identify at least one ligand, the method comprising:
a) combining the protein of claim 2 with the molecules or compounds under conditions to allow specific binding; and b) detecting specific binding, thereby identifying a ligand which specifically binds the protein.
- 5. The method of claim 4 wherein the molecules or compounds are selected from DNA molecules, RNA molecules, peptide nucleic acids, peptides, proteins, mimetics, agonists, antagonists, antibodies, immunoglobulins, inhibitors, and drugs.
- 6. An isolated antibody which specifically binds to a protein of claim 2.
- 7. The antibody of claim 6, wherein the antibody is selected from an intact immunoglobulin molecule, a polyclonal antibody, a monoclonal antibody, a chimeric antibody, a recombinant antibody, a humanized antibody, a single chain antibody, a Fab fragment, and a F(ab′)2 fragment.
- 8. A method of using a protein to prepare and purify a polyclonal antibody comprising:
a) immunizing a animal with the protein of claim 2 under conditions to elicit an antibody response; b) isolating animal antibodies; c) attaching the protein to a substrate; d) contacting the substrate with isolated antibodies under conditions to allow specific binding to the protein; e) dissociating the antibodies from the protein, thereby obtaining purified polyclonal antibodies.
- 9. A polyclonal antibody produced by the method of claim 8.
- 10. A method of using a protein to prepare a monoclonal antibody comprising:
a) immunizing a animal with a protein of claim 2 under conditions to elicit an antibody response; b) isolating antibody-producing cells from the animal; c) fusing the antibody-producing cells with immortalized cells in culture to form monoclonal antibody producing hybridoma cells; d) culturing the hybridoma cells; and e) isolating monoclonal antibodies from culture.
- 11. A monoclonal antibody produced by the method of claim 10.
- 12. A method for using an antibody to diagnose conditions or diseases associated with expression of a protein, the method comprising:
a) combining the antibody of claim 6 with a sample, thereby forming antibody:protein complexes; and b) comparing complex formation with a standard, wherein the comparison indicates expression of the protein in the sample.
- 13. The method of claim 12 wherein expression is diagnostic of a cancer, particularly intestine cancer, breast cancer, uterine cancer, liver cancer, brain cancer, and kidney cancer.
- 14. A composition comprising an antibody of claim 6 and a labeling moiety.
- 15. A composition comprising an antibody of claim 6 and a pharmaceutical agent.
- 16. A method for using an antibody to immunopurify a protein comprising:
a) attaching the antibody of claim 6 to a substrate, b) exposing the antibody to a sample containing protein under conditions to allow antibody:protein complexes to form, c) dissociating the protein from the complex, and d) collecting the purified protein.
- 17. A method of using an antibody to treat a cancer, particularly a cancer of the intestine, breast, uterus, liver, brain, or kidney comprising administering to a patient in need of such treatment the composition of claim 15.
Parent Case Info
[0001] This application is a divisional application of U.S. application Ser. No. 09/812,484, filed Mar. 19, 2002, entitled NDR2-RELATED PROTEINS, which is a continuation-in-part of U.S. application Ser. No. 09/232,160, filed Jan. 15, 1999, now U.S. Pat. No. 6,368,794, issued Apr. 9, 2002, entitled DETECTION OF ALTERED EXPRESSION OF GENES REGULATING CELL PROLIFERATION, all of which applications and patents are hereby incorporated by reference.
Divisions (1)
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Number |
Date |
Country |
Parent |
09812484 |
Mar 2001 |
US |
Child |
10172069 |
Jun 2002 |
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09232160 |
Jan 1999 |
US |
Child |
09812484 |
Mar 2001 |
US |