Claims
- 1. An isolated cDNA comprising a nucleic acid sequence encoding a protein selected from the amino acid sequence of SEQ ID NO:1 and SEQ ID NO:2, or the complement thereof.
- 2. An isolated cDNA comprising a nucleic acid sequence selected from:
a) SEQ ID NO:3 and SEQ ID NO:11 or the complement thereof; b) a fragment of SEQ ID NO:3 selected from SEQ ID NOs:4-10 or the complement thereof; c) a fragment of SEQ ID NO:11 selected from SEQ ID NOs:12-15 or the complement thereof; and d) a variant of SEQ ID NO:3 or SEQ ID NO:11 selected from SEQ ID NOs:16-31 or the complement thereof.
- 3. An isolated cDNA comprising the nucleic acid sequence of SEQ ID NO:3.
- 4. An isolated cDNA comprising the nucleic acid sequence of SEQ ID NO:11.
- 5. A composition comprising the cDNA or the complement of the cDNA of claim 1 and a labeling moiety.
- 6. A vector comprising the cDNA of claim 1.
- 7. A host cell comprising the vector of claim 6.
- 8. A method for using a cDNA to produce a protein, the method comprising:
a) culturing the host cell of claim 7 under conditions for protein expression; and b) recovering the protein from the host cell culture.
- 9. A method for using a cDNA to detect expression of a nucleic acid in a sample comprising:
a) hybridizing the composition of claim 5 to nucleic acids of the sample, thereby forming hybridization complexes; and b) comparing hybridization complex formation with a standard, wherein the comparison indicates expression of the cDNA in the sample.
- 10. The method of claim 9 further comprising amplifying the nucleic acids of the sample prior to hybridization.
- 11. The method of claim 9 wherein the composition is attached to a substrate.
- 12. The method of claim 9 wherein the cDNA is differentially expressed when compared with a standard and is diagnostic of a cancer, particularly intestine cancer, breast cancer, uterine cancer, liver cancer, brain cancer, and kidney cancer.
- 13. A method of using a cDNA to screen a plurality of molecules or compounds, the method comprising:
a) combining the cDNA of claim 1 with a plurality of molecules or compounds under conditions to allow specific binding; and b) detecting specific binding, thereby identifying a molecule or compound which specifically binds the cDNA.
- 14. The method of claim 13 wherein the molecules or compounds are selected from DNA molecules, RNA molecules, peptide nucleic acids, artificial chromosome constructions, peptides, transcription factors, repressors, and regulatory molecules.
- 15. A purified protein or a portion thereof produced by the method of claim 8 and selected from:
a) an amino acid sequence of SEQ ID NO:1 and SEQ ID NO:2; b) an antigenic epitope of SEQ ID NO:1 or SEQ ID NO:2; and c) a biologically active portion of SEQ ID NO:1 or SEQ ID NO:2.
- 16. A composition comprising the protein of claim 15 and a pharmaceutical carrier.
- 17. A method for using a protein to screen a plurality of molecules or compounds to identify at least one ligand, the method comprising:
a) combining the protein of claim 15 with the molecules or compounds under conditions to allow specific binding; and b) detecting specific binding, thereby identifying a ligand which specifically binds the protein.
- 18. The method of claim 17 wherein the molecules or compounds are selected from DNA molecules, RNA molecules, peptide nucleic acids, peptides, proteins, mimetics, agonists, antagonists, antibodies, immunoglobulins, inhibitors, and drugs.
- 19. A method of using a protein to prepare and purify antibodies comprising:
a) immunizing a animal with the protein of claim 15 under conditions to elicit an antibody response; b) isolating animal antibodies; c) attaching the protein to a substrate; d) contacting the substrate with isolated antibodies under conditions to allow specific binding to the protein; e) dissociating the antibodies from the protein, thereby obtaining purified antibodies.
- 20. An antibody produced by the method of claim 19.
- 21. A method for using an antibody to diagnose conditions or diseases associated with expression of a protein, the method comprising:
a) combining the antibody of claim 20 with a sample, thereby forming antibody:protein complexes; and b) comparing complex formation with a standard, wherein the comparison indicates expression of the protein in the sample.
- 22. The method of claim 21 wherein expression is diagnostic of a cancer, particularly intestine cancer, breast cancer, uterine cancer, liver cancer, brain cancer, and kidney cancer.
Parent Case Info
[0001] This application is a continuation-in-part of copending U.S. Ser. No. 09/232,160, Incyte Docket No. PA-0003 U.S., filed Jan. 15, 1999, all of which application is hereby incorporated by reference.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09232160 |
Jan 1999 |
US |
Child |
09812484 |
Mar 2001 |
US |