Claims
- 1. An isolated nucleic acid molecule selected from the group consisting of:
a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10 or SEQ ID NO:22, or a complement thereof; b) a nucleic acid molecule comprising a fragment of at least 100 contiguous nucleotides of a nucleic acid comprising the nucleotide sequence of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:22, or a complement thereof; c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21; d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21, wherein the fragment comprises at least 15 contiguous amino acid residues of the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21; and e) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:22 under stringent conditions.
- 2. The nucleic acid molecule of claim 1 further comprising vector nucleic acid sequences.
- 3. The nucleic acid molecule of claim 1 further comprising nucleic acid sequences encoding a heterologous polypeptide.
- 4. A host cell which contains the nucleic acid molecule of claim 1.
- 5. The host cell of claim 4 which is a mammalian host cell.
- 6. A non-human mammalian host cell containing the nucleic acid molecule of claim 1.
- 7. An isolated polypeptide selected from the group consisting of:
a) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21; b) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:22 under stringent conditions; c) a polypeptide which is encoded by the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:22; and d) a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21.
- 8. The polypeptide of claim 7 further comprising heterologous amino acid sequences.
- 9. An antibody which selectively binds to a polypeptide of claim 7.
- 10. A method for producing a polypeptide selected from the group consisting of:
a) a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21; b) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21; and c) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:5, SEQ ID NO:8 or SEQ ID NO:21, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:10, or SEQ ID NO:22 under stringent conditions; comprising culturing the host cell of claim 4 under conditions in which the nucleic acid molecule is expressed.
- 11. A method for detecting the presence of a polypeptide of claim 7 in a sample comprising:
a) contacting the sample with a compound which selectively binds to the polypeptide; and b) determining whether the compound binds to the polypeptide in the sample to thereby detect the presence of a polypeptide of claim 7 in the sample.
- 12. The method of claim 11, wherein the compound which binds to the polypeptide is an antibody.
- 13. A kit comprising a compound which selectively binds to a polypeptide of claim 7 and instructions for use.
- 14. A method for detecting the presence of a nucleic acid molecule in claim 1 in a sample comprising:
a) contacting the sample with a nucleic acid probe or primer which selectively hybridizes to the nucleic acid molecule; and b) determining whether the nucleic acid probe or primer binds to a nucleic acid molecule in the sample to thereby detect the presence of a nucleic acid molecule of claim 1 in the sample.
- 15. The method of claim 14, wherein the sample comprises mRNA molecules and is contacted with a nucleic acid probe.
- 16. A kit comprising a compound which selectively hybridizes to a nucleic acid molecule of claim 1 and instructions for use.
- 17. A method for identifying a compound which modulates binding of NEOKINE to NEOKINE receptor comprising:
a) contacting a cell which expresses NEOKINE receptor with NEOKINE and a test compound under conditions which allow NEOKINE to bind to NEOKINE receptor; and b) detecting binding of NEOKINE in the presence of the test compound; wherein a change in the amount of NEOKINE binding to NEOKINE receptor detected in the presence of the test compound indicates that the test compound modulates binding of NEOKINE to NEOKINE receptor.
- 18. A method for identifying a compound which modulates the activity of NEOKINE receptor comprising:
a) contacting a cell which expresses NEOKINE receptor with NEOKINE and a test compound under conditions which allow NEOKINE to bind to NEOKINE receptor; and b) detecting the activity of NEOKINE receptor in the presence of the test compound; wherein a change in the activity NEOKINE receptor detected in the presence of the test compound indicates that the test compound modulates the activity of NEOKINE receptor.
- 19. A method for identifying a compound which modulates binding of NEOKINE to NEOKINE receptor comprising:
a) contacting NEOKINE receptor with NEOKINE and a test compound under conditions which allow NEOKINE to bind to NEOKINE receptor; and b) detecting binding of NEOKINE in the presence of the test compound; wherein a change in the amount of NEOKINE binding to NEOKINE receptor detected in the presence of the test compound indicates that the test compound modulates binding of NEOKINE to NEOKINE receptor.
- 20. The method of any of claims 17 to 19 wherein the NEOKINE receptor is selected from the group consisting of:
a) a receptor having an amino acid sequence which is substantially homologous to the amino acid sequence of SEQ ID NO:16, SEQ ID NO:18, or SEQ ID NO:20; b) a receptor which is encoded by an isolated nucleic acid molecule which is substantially homologous to the nucleic acid sequence of SEQ ID NO:15, SEQ ID NO:17, or SEQ ID NO:19; and c) a receptor which is encoded by an isolated nucleic acid molecule which hybridizes under stringent conditions to a nucleic acid molecule having the nucleic acid sequence of SEQ ID NO:15, SEQ ID NO:17, or SEQ ID NO:17.
- 21. The method of any of claims 17 to 19 wherein the NEOKINE is selected form the group consisting of:
a) a polypeptide having an amino acid sequence which is substantially homologous to the amino acid sequence of SEQ ID NO:2, SEQ ID NO:5, or SEQ ID NO:8; b) a polypeptide which is encoded by an isolated nucleic acid molecule which is substantially homologous to the nucleic acid sequence of SEQ ID NO:15, SEQ ID NO:17, or SEQ I) NO:19; and c) a polypeptide which is encoded by an isolated nucleic acid molecule which hybridizes under stringent conditions to a nucleic acid molecule having the nucleic acid sequence of SEQ ID NO:15, SEQ ID NO:17, or SEQ ID NO:17.
- 22. A method of modulating the activity of a polypeptide of claim 7 comprising contacting the polypeptide or a cell expressing the polypeptide with a compound which binds to the polypeptide in a sufficient concentration to modulate the activity of the polypeptide.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a continuation-in-part of prior-filed application U.S. patent application Ser. No. 09/023,664 entitled “Novel Antikine Protein and Nucleic Acid Molecules and Uses Therefor”, filed Feb. 10, 1998. The content of the above-referenced patent application is incorporated herein by this reference in its entirety.
Continuations (1)
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Number |
Date |
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Parent |
09248239 |
Feb 1999 |
US |
Child |
09940240 |
Aug 2001 |
US |
Continuation in Parts (1)
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Number |
Date |
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Parent |
09023664 |
Feb 1998 |
US |
Child |
09248239 |
Feb 1999 |
US |