Claims
- 1. A method for screening for the presence of an amplicon in a sample of human nucleic acid, the method comprising:
providing a sample of nucleic acid derived from a human cell and a probe, wherein the probe comprises nucleic acid which hybridizes specifically to a nucleic acid sequence including from D20S211 through D20S 120; contacting the human nucleic acid with the probe, wherein the probe is contacted with the human genomic nucleic acid under conditions in which the probe binds selectively under stringent conditions to the human genomic nucleic acid to form a hybridization complex; and detecting the formation of the hybridization complex.
- 2. The method of claim 1, wherein the human nucleic acid is a genomic DNA.
- 3. The method of claim 1, wherein the step of detecting the hybridization complex further comprises determining the copy number of the amplicon.
- 4. The method of claim 1, wherein the genomic nucleic acid is isolated from a breast tumor cell.
- 5. The method of claim 1, wherein the probe comprises a nucleic acid which hybridizes specifically to a nucleic acid sequence spanning the distance between D20S120 and D20S211.
- 6. The method of claim 1, wherein the probe comprises a nucleic acid which hybridizes specifically to a STS marker selected from the group consisting of AFMa233wg1, AFM080ya1, AFM069ya1, WI-16748, WI-9939, AFMa072zb9, WI-6578, AFM224zd12, WI-9227, and AFM276xh1.
- 7. The method of claim 1, wherein the probe comprises nucleic acid which hybridizes specifically to a GDB locus nucleic acid sequence selected from the group consisting of D20S211, D20S854, D20S876, D20S1044, D20S913, D20S720, and D20S120.
- 8. The method of claim 1, wherein the probe comprises nucleic acid which hybridizes specifically to a cloned genomic nucleic acid sequence selected from the group consisting of RMC20B4097, RMC20B4103, RMC20P4016, RMC20B4130, RMC20P4185, RMC20B4188, RMC20B4109, RMC20P4010, RMC20P4028, RMC20P4003, RMC20B4099, RMC20P4018, RMC20P4069, RMC20B4121, RMC20B4087, and RMC20P4070.
- 9. The method of claim 1, wherein the probe comprises a polymerase chain reaction primer pair capable of amplifying some or all of the nucleic acid sequence including from D20S211 through D20S120 and the detection step comprises detecting the formation of the polymerase chain reaction amplification reaction.
- 10. The method of claim 9, wherein the polymerase chain reaction primer pair is an STS PCR primer pair selected from the group consisting of AFMa233wg1, AFM080ya1, AFM069ya1, WI-16748, WI-9939, AFMa072zb9, WI-6578, AFM224zd12, WI-9227, and AFM276xh1.
- 11. The method of claim 1, wherein the probe is attached to a solid surface.
- 12. The method of claim 11, wherein the attached probe is a member of a nucleic acid array.
- 13. The method of claim 1, wherein the human nucleic acid is labeled with a detectable composition.
- 14. The method of claim 13, wherein the detectable composition is fluorescein or Texas red.
- 15. The method of claim 1, wherein the probe is labeled with a detectable composition.
- 16. The method of claim 1, wherein the method further provides nucleic acids from a reference cell, wherein the reference cell nucleic acid is contacted with the probe before or simultaneously with the human genomic nucleic acid.
- 17. The method of claim 1, wherein the method further provides Cot-1 DNA, wherein the Cot-1 DNA is hybridized to the human genomic nucleic acid before contacting the human genomic nucleic acid with the probe.
- 18. A nucleic acid probe for screening for the presence of an amplicon in a sample of human genomic nucleic acid, comprising a nucleic acid which hybridizes specifically to a nucleic acid sequence including from D20S211 through D20S120.
- 19. The probe of claim 18, wherein the probe comprises nucleic acid which hybridizes specifically to the nucleic acid sequence spanning the distance between D20S120 and D20S211.
- 20. The probe of claim 18, wherein the probe comprises a nucleic acid which hybridizes specifically to a STS marker selected from the group consisting of AFMa233wg1, AFM080ya1, AFM069ya1, WI-16748, WI-9939, AFMa072zb9, WI-6578, AFM224zd12, WI-9227, and AFM276xh1.
- 21. The probe of claim 18, wherein the probe comprises nucleic acid which hybridizes specifically to a GDB locus nucleic acid sequence selected from the group consisting of D20S211, D20S854, D20S876, D20S1044, D20S913, D20S720, and D20S8120.
- 22. The probe of claim 18, wherein the probe comprises nucleic acid which hybridizes specifically to a cloned genomic nucleic acid sequence selected from the group consisting of RMC20B4097, RMC20B4103, RMC20P4016, RMC20B4130, RMC20P4185, RMC20B4188, RMC20B4109 , RMC20WP4010, RMC20P4028, RMC20P4003, RMC20B4099, RMC20P4018, RMC20P4069, RMC20B4121, RMC20B4087, and RMC20P4070.
- 23. The probe of claim 18, wherein the probe comprises a polymerase chain reaction primer pair capable of amplifying some or all of the nucleic acid sequence including from D20S211 through D20S120.
- 24. The probe of claim 23, wherein the polymerase chain reaction primer pair is an STS PCR primer pair selected from the group consisting of AFMa233wg1, AFM080ya1, AFM069ya1, WI-16748, WI-9939, AFMa072zb9, WI-6578, AFM224zd12, WI-9227, and AFM276xh1.
- 25. A kit for screening for the presence of an amplicon in a sample of human nucleic acid, the kit comprises a compartment which contains a probe, wherein the probe comprises nucleic acid which hybridizes specifically to a nucleic acid sequence including from D20S211 through D20S120.
- 26. The kit of claim 25, wherein the probe comprises nucleic acid which spans the distance between D20S120 and D20S211.
- 27. The kit of claim 25, wherein the probe comprises a nucleic acid which hybridizes specifically to a STS marker selected from the group consisting of AFMa233wg1, AFM080ya1, AFM069ya1, WI-16748, WI-9939, AFMa072zb9, WI-6578, AFM224zd12, WI-9227, and AFM276xh1.
- 28. The kit of claim 25, wherein the probe comprises nucleic acid which hybridizes specifically to a GDB locus nucleic acid sequence selected from the group consisting of D20S211, D20S854, D20S876 , D20P1044, D20S913, D20P720, and D20S120.
- 29. The kit of claim 25, wherein the probe comprises nucleic acid which hybridizes specifically to a cloned genomic nucleic acid sequence selected from the group consisting of RMC20B4097, RMC20B4103, RMC20P4016, RMC20B4130, RMC20P4185, RMC20B4188, RMC20B4109, RMC20P4010, RMC20P4028, RMC20P4003, RMC20B4099, RMC20P4069, RMC20P4018, RMC20B4121, RMC20B4087, and RMC20P4070.
- 30. The kit of claim 25, wherein the probe comprises a polymerase chain reaction primer pair capable of amplifying some or all of the nucleic acid sequence including from D20S211 through D20S120.
- 31. The kit of claim 30, wherein the polymerase chain reaction primer pair is an STS PCR primer pair selected from the group consisting of AFMa233wg1, AFM080ya1, AFM069ya1, WI-16748, WI-9939, AFMa072zb9, WI-6578, AFM224zd12, WI-9227, and AFM276xh1.
- 32. The kit of claim 25, wherein the probe is a cloned human nucleic acid.
- 33. The kit of claim 25, wherein the cloned human genomic nucleic acid is attached to a solid surface.
- 34. The kit of claim 33, wherein the attached probe is a member of a nucleic acid array.
- 35. The kit of claim 25, wherein the kit further comprises instructional material that indicates that the detection of greater than two amplicon copies in a cell can be diagnostic or prognostic of cancer or tumorigenesis.
Parent Case Info
[0001] The present application is related to U.S. patent application Ser. No. (“USSN”) 08/680,395, filed Jul. 15, 1996; and U.S. Ser. No. 08/731,499, filed Oct. 16, 1996. The present application also incorporates by reference each of the aforementioned applications in their entirety and for all purposes.
Government Interests
[0002] This invention was made with United States Government support under Grant No. NIH/NCI 5P50CA-58207-06, awarded by the National Institutes of Health. The United States Government has certain rights in this invention.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09134044 |
Aug 1998 |
US |
Child |
09896070 |
Jun 2001 |
US |