Claims
- 1. A method of producing collagen comprising:
providing collagen-containing tissues; providing microorganisms; and allowing the microorganisms to ferment the collagen-containing tissues.
- 2. The method of claim 1, wherein the microorganisms comprise bacteria.
- 3. The method of claim 2, wherein the bacteria are Gram positive.
- 4. The method of claim 3, wherein the bacteria are of the genus Bacillus.
- 5. The method of claim 1, wherein the microorganisms comprise yeast.
- 6. The method of claim 1, wherein the microorganisms comprises GRAS microorganisms.
- 7. The method of claim 6, wherein the GRAS microorganisms are Gram positive.
- 8. The method of claim 1, wherein the collagen-containing tissues are obtained from mammals.
- 9. The method of claim 8, wherein the mammals are porcine animals.
- 10. The method of claim 1, wherein the collagen-containing tissues are obtained from aquatic animals.
- 11. The method of claim 10, wherein the aquatic animal is fish.
- 12. The method of claim 11, wherein the fish is shark.
- 13. The method of claim 1, wherein the collagen-containing tissues are obtained from birds.
- 14. The method of claim 13, wherein the birds are chickens.
- 15. The method of claim 1, further comprising extracting collagen from the fermented tissues.
- 16. The method of claim 15, further comprising dissolving the fermented tissues in acidic solutions.
- 17. The method of claim 16, further comprising removing insoluble tissues by filtration.
- 18. The method of claim 17, further comprising adding salt to the acidic solutions containing the fermented tissues to precipitate the collagen.
- 19. The method of claim 18, further comprising collecting the precipitated collagen by filtration.
- 20. The method of claim 15, wherein the collagen-containing tissues are obtained from mammals.
- 21. The method of claim 20, wherein the mammals are porcine animals.
- 22. The method of claim 15, wherein the collagen-containing tissues are obtained from aquatic animals.
- 23. The method of claim 22, wherein the aquatic animal is fish.
- 24. The method of claim 23, wherein the fish is shark.
- 25. The method of claim 15, wherein the collagen-containing tissues are obtained from birds.
- 26. The method of claim 25, wherein the birds are chickens.
- 27. The method of claim 15, further comprising the step of hydrolyzing the extracted collagen.
- 28. A method of producing collagen comprising:
providing collagen-containing tissues from one or more of mammalian, avian, or aquatic animal sources; providing Gram (+) bacteria belonging to the genus Bascillus; fermenting the collagen-containing tissues at about 10% w/v in a fermenter with the bacteria; dissolving fermented tissues in an acidic solution with an enzyme; removing insoluble tissues by filtration; and adding salt to the acidic solution sufficient to precipitate collagen and keeping it undisturbed overnight to precipitate the collagen.
- 29. The method of claim 28, wherein the collagen-containing tissues is fermented with about 160 μl bacteria.
- 30. The method of claim 28, wherein the acidic solution is about 1% w/v to about 50% w/v of 0.5M acetic acid (pH 3.0).
- 31. The method of claim 28, wherein the enzyme is pepsin at about 0.2% to about 5% w/v.
- 32. The method of claim 28, wherein the salt is about 400 to 600 grams.
- 33. A method of producing collagen comprising:
providing collagen-containing tissues from an avian source; providing Gram (+) bacteria belonging to the genus Bascillus; fermenting the collagen-containing tissues at about 10% w/v with about 160 μl of the bacteria and nutrient medium in a fermenter for about 24 hours; dissolving fermented tissues at about 3% w/v in an acidic solution with about 0.5M acetic acid (about pH3.0) and about 1% w/v pepsin and stirring for about 48 hours; removing insoluble tissues by filtration; and adding about 560 grams of salt to the acidic solution sufficient to precipitate collagen and keeping it undisturbed overnight to precipitate the collagen.
- 34. The method of claim 33, wherein the avian source is chicken.
- 35. A method of producing collagen comprising the steps of:
providing collagen-containing tissues from one or more of mammalian, avian, or aquatic animal sources; providing Gram (+) bacteria belonging to the genus Bacillus; fermenting the collagen-containing tissues at about 10% to about 40% w/v with about 10 μl of the bacteria in a fermenter; and dissolving fermented tissues in acidic solution with about 0.5M acetic acid (about pH3.0) and pepsin.
- 36. The method of claim 35, wherein the collagen-containing tissues is fermented for about 18 to about 48 hours.
- 37. The method of claim 35, wherein fermented tissue is about 1% w/v to about 50% w/v of the acidic solution.
- 38. The method of claim 35, wherein the pepsin is about 0.2% to about 5% w/v.
- 39. A method of producing collagen comprising the steps of:
providing collagen-containing tissues from a mammalian source; providing Gram (+) bacteria belonging to the genus Bacillus; fermenting the collagen-containing tissues in a fermenter (about 10% to about 40% w/v) for about 18 to about 48 hours with about 10 μl of the bacteria and nutrient medium; dissolving fermented tissues in aqueous solution (about 3% w/v) containing about 0.5M acetic acid (about pH3.0) and about 0.4% to about 2% w/v pepsin and stirring for not more than 48 hours.
- 40. The method of claim 39, wherein the mammalian source is porcine.
- 41. A collagen product comprising collagen monomers.
- 42. The collagen product of claim 41, wherein the collagen monomers is at least about 10% by weight of the weight of total collagen in the collagen product.
- 43. The collagen product of claim 41, wherein the collagen monomers is at least about 50% by weight of the weight of the total collagen in the collagen product.
- 44. The collagen product of claim 41, wherein the collagen monomers is at least about 80% by weight of the weight of the total collagen in the collagen product.
- 45. The collagen product of claims 41, 42, 43 or 44, wherein the collagen monomers are obtained from collage-containing tissues through a fermentation process.
- 46. The collagen product of claim 45, wherein the fermentation process is carried out with microorganisms comprising bacteria.
- 47. The collagen product of claim 45, wherein the fermentation process is carried out with microorganisms comprising yeast.
- 48. The collagen product of claim 45, wherein the fermentation process is carried out with microorganisms comprising GRAS microorganisms.
- 49. The collagen product of claim 48, wherein the GRAS microorganisms are Gram positive microorganisms.
- 50. The collagen product of claim 45, wherein the collagen-containing tissues are obtained from mammals.
- 51. The collagen product of claim 45, wherein the collagen-containing tissues are obtained from aquatic animals.
- 52. The collagen product of claim 51, wherein the aquatic animal is fish.
- 53. The collagen product of claim 45, wherein the collagen-containing tissues are obtained from birds.
DESCRIPTION OF THE INVENTION
[0001] This application claims the benefit of U.S. Provisional Application No. 60/462,528, filed Apr. 11, 2003, which is incorporated herein by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60462528 |
Apr 2003 |
US |