Claims
- 1. An isolated nucleic acid encoding an estrogen-regulated GTP-binding protein gamma-12 subunit protein, wherein the protein comprises the amino acid sequence of SEQ ID NO: 1.
- 2. The nucleic acid of claim 1, wherein the nucleic acid is from a mouse.
- 3. The nucleic acid of claim 1, wherein the nucleic acid comprises a nucleotide sequence that is at least about 70% identical to SEQ ID NO: 2 or 3.
- 4. The nucleic acid of claim 1, wherein the nucleic acid comprises the nucleotide sequence of SEQ ID NO: 2 or 3.
- 5. An expression cassette comprising the nucleic acid of claim 1.
- 6. An isolated eukaryotic cell comprising the expression cassette of claim 5.
- 7. An isolated estrogen-regulated GTP-binding protein gamma-12 subunit protein, wherein the protein comprises the amino acid sequence of SEQ ID NO: 1.
- 8. The protein of claim 7, wherein the protein is a mouse protein.
- 9. An antibody that selectively binds to the estrogen-regulated GTP-binding protein gamma-12 subunit protein of claim 7, wherein the antibody does not bind to the estrogen-regulated GTP-binding protein gamma-12 subunit protein having the amino acid sequence of SEQ ID NO: 4.
- 10. A method of modulating estrogen signaling in a mammalian cell, the method comprising modulating the level of expression or activity of an estrogen-regulated GTP-binding protein gamma-12 subunit protein.
- 11. The method of claim 10, wherein said level of expression of said estrogen-regulated GTP-binding protein gamma-12 subunit protein is modulated by introducing a polynucleotide into said cell, whereby the presence or expression of said polynucleotide modulates said level of expression of said estrogen-regulated GTP-binding protein gamma-12 subunit protein.
- 12. The method of claim 11, wherein said polynucleotide encodes a full-length estrogen-regulated GTP-binding protein gamma-12 subunit protein, and wherein expression of said polynucleotide increases said level of expression of said estrogen-regulated GTP-binding protein gamma-12 subunit protein.
- 13. The method of claim 11, wherein said polynucleotide is an antisense sequence, and wherein the presence or expression of said polynucleotide decreases said level of expression of said estrogen-regulated GTP-binding protein gamma-12 subunit protein.
- 14. The method of claim 10, wherein a compound is administered to said cell, whereby said level of said expression or activity of said estrogen-regulated GTP-binding protein gamma-12 subunit is modulated.
- 15. The method of claim 10, wherein the estrogen signaling is mediated by an estrogen receptor alpha.
- 16. The method of claim 10, wherein the cell is present in a mammal.
- 17. The method of claim 16, wherein the cell is a vascular smooth muscle cell or a vascular endothelial cell.
- 18. The method of claim 16, wherein said level of expression or activity of said estrogen-regulated GTP-binding protein gamma-12 subunit protein is increased, whereby the development of atherosclerosis, osteoporosis, Alzheimer's disease or Parkinson's disease is inhibited in said mammal.
- 19. A method of detecting the presence of estrogen signaling in a mammalian cell, the method comprising detecting the expression of a nucleic acid encoding an estrogen-regulated GTP-binding protein gamma-12 subunit protein.
- 20. The method of claim 19, wherein said nucleic acid is the nucleic acid of claim 1.
- 21. The method of claim 19, wherein said presence of estrogen signaling in said cell is used in order to determine the responsiveness of said cell to estrogen.
- 22. The method of claim 19, wherein said presence of estrogen signaling in said cell is used in order to determine the tissue-specific distribution of estrogen signaling in a mammal.
- 23. The method of claim 19, wherein said expression of said nucleic acid in said cell is detected by detecting the expression or activity of an estrogen-regulated GTP-binding protein gamma-12 subunit protein.
- 24. The method of claim 19, wherein said protein is the protein of claim 7.
- 25. The method of claim 19, wherein said expression of said nucleic acid in said cell is detected by detecting the level of estrogen-regulated GTP-binding protein gamma-12 subunit mRNA in said cell.
- 26. The method of claim 19, wherein the estrogen signaling is mediated by an estrogen receptor alpha.
- 27. A method of identifying a compound capable of acting as an estrogen receptor agonist or antagonist, the method comprising:
(1) contacting a cell comprising an estrogen receptor with said compound; and (2) determining the functional effect of said compound on said cell, wherein an increase in the level of estrogen-regulated GTP-binding protein gamma-12 subunit mRNA, protein or protein activity in said cell indicates that said compound is capable of acting as an estrogen receptor agonist, and wherein a decrease in the level of estrogen-regulated GTP-binding protein gamma-12 subunit mRNA, protein or protein activity in said cell indicates that said compound is capable of acting as an estrogen receptor antagonist.
- 28. The method of claim 27, wherein the estrogen receptor is an estrogen receptor alpha.
- 29. The method of claim 27, wherein said estrogen-regulated GTP-binding protein gamma-12 subunit mRNA has the sequence of SEQ ID NO: 1, or wherein said estrogen-regulated GTP-binding protein gamma-12 subunit protein comprises the amino acid sequence of SEQ ID No: 2 or 3.
CROSS REFERENCE TO RELATED APPLICATION
[0001] This application claims benefit of U.S. Provisional Application No. 60/188,460, filed Mar. 10, 2000, which application is incorporated herein by reference for all purposes.
Provisional Applications (1)
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Number |
Date |
Country |
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60188460 |
Mar 2000 |
US |