Patent Application
20150337331
The present invention relates to a novel expression vector for efficient expression of recombinant proteins in mammalian cells, and in particular to an expression vector that comprises a gene expression regulatory site, a gene encoding a protein of interest downstream thereof, an internal ribosome entry site further downstream thereof, a gene encoding a glutamine synthetase still further downstream thereof, and a dihydrofolate reductase gene downstream of either the same gene expression regulatory site or another gene expression regulatory site in addition to the former.
In some fields of industry such as drug manufacturing, a familiar technology is a method for production of a recombinant protein of interest using mammalian cells transformed with an expression vector that contains an incorporated gene encoding the protein. Using this technology, various products are produced and marketed, e.g., lysosomal enzymes such as α-galactosidase A, iduronate-2-sulfatase, glucocerebrosidase, galsulfase, α-L-iduronidase, α-glucosidase, and the like; tissue plasminogen activator (t-PA); blood coagulation factors such as blood coagulation factor VII, blood coagulation factor VIII, blood coagulation factor IX, and the like; erythropoietin; interferon; thrombomodulin; follicle-stimulating hormone; granulocyte colony-stimulating factor (G-CSF); various antibody medicaments, and the like.
In performing this technology, it is a general practice to employ an expression vector in which a gene encoding a protein of interest is incorporated downstream of a gene regulatory site that induces a potent expression of a gene, such as a cytomegalovirus (CMV)-derived promoter, SV40 early promoter (SV40 enhancer/promoter), or elongation factor in (EF-1) promoter. Mammalian cells, after introduction therein of such an expression vector, come to express the protein of interest that is incorporated in the expression vector. The levels of its expression, however, vary and are not even among those cells. Therefore, for efficient production of the recombinant protein, a step is required to select, from the mammalian cells containing the expression vector introduced therein, those cells which express the protein of interest at high levels. For performing this selection step, a gene that acts as a selection marker is incorporated in an expression vector.
The most popular of such selection markers are enzymes (drug resistance markers) that decompose drugs such as puromycin, neomycin, and the like. Mammalian cells will be killed in the presence of these drugs beyond certain concentrations. Mammalian cells into which an expression vector has been introduced, however, become viable in the presence of those drugs because such cells can decompose the drugs with the drug selection markers incorporated in the expression vector and thus detoxify them or weaken their toxicity. Therefore, when those cells having such an incorporated expression marker are cultured in a medium containing one of the above mentioned drugs beyond a certain concentration, only such cells grow that express the corresponding selection marker at high levels, and as a result, they are selected. Such cells that express a drug selection marker at high levels also tend to express, at high levels, a gene encoding a protein of interest incorporated together in the expression vector, and as a result, mammalian cell thus will be obtained that express the protein of interest at high levels.
There is also known a method to obtain mammalian cells that express a protein of interest at high levels utilizing dihydrofolate reductase (DHFR) as a selection marker (Non-patent Document 1). Dihydrofolate reductase is an enzyme which reduces dihydrofolate to tetrahydrofolate. Mammalian cells will die if they are cultured in a thymidine-hypoxanthine-free medium in the presence of methotrexate (MTX), an inhibitor of DHFR, beyond a certain concentration. However, if an expression vector containing an incorporated DHFR gene as a selection marker is introduced into mammalian cells, they become capable of growing even at higher concentrations of MTX because of elevated expression levels of DHFR in them. In this circumstance, if culture is continued gradually elevating the MTX concentration, such cells are obtained that can grow in the presence of even higher concentrations of MTX. This phenomenon is thought to occur because of increase in number of the copies of the expression vector incorporated into the genome of the mammalian cells by multiplication. That is, an increase in number of copies of the expression vector leads to a corresponding increase in number of the DHFR genes in the genome of each cell, resulting in relatively enhanced levels of expression of DHFR. In this process, the number of copies of the gene encoding a protein of interest and simultaneously incorporated in the expression vector also increases, and thus gives mammalian cells that express the protein of interest at high levels.
Expression vectors are also known in which a glutamine synthetase (GS) is used as a selection marker (cf. Patent Documents 1 and 2). Glutamine synthetase is an enzyme which synthesizes glutamine from glutamic acid and ammonia. If mammalian cells are cultured in a medium which lacks glutamine in the presence of methionine sulfoximine (MSX), an inhibitor of glutamine synthetase, beyond a certain concentration, the cells will be annihilated. However, if an expression vector into which a glutamine synthetase has been incorporated as a selection marker is introduced into mammalian cells, the cells, now with increased levels of expression of the glutamine synthetase, become capable of growing even in the presence of higher concentrations of MSX. In doing this, if culture is continued with a gradually increasing concentration of MSX, such cells are obtained that can grow in the presence of still higher concentrations of MSX. This phenomenon occurs in the same mechanism as where DHFR is used as a selection marker. Therefore, by incorporating in an expression vector a gene encoding a protein of interest together with a GS gene, such mammalian cells will be obtained that express the protein of interest at high levels. For example, Patent Document 1 discloses that by employment of a GS gene and methionine sulfoximine (MSX) enables greater increase of the copy number of the vector DNA than where DHFR gene and methotrexate (MTX) are employed. Further, Patent Document 2 discloses that by employment of a GS gene and MSX, the copy number of a different, heterozygous gene can also be increased, along with increased number of copies of the GS gene, which thereby enables increased production levels of a polypeptide of interest.
Thus, expression vectors containing a selection marker are suitable for efficient production of recombinant proteins, and thus are commonly used. A gene encoding a protein of interest and a gene encoding a selection marker are generally incorporated in an expression vector downstream of respective different gene regulatory sites (cf. Patent Document 3). However, a method is also known in which genes encoding a protein of interest and a selection marker are incorporated in series downstream of a single gene regulatory site to let them express themselves (cf. Patent Documents 4-7). In performing this, an internal ribosome entry site (IRES) and the like are inserted between the genes encoding a protein of interest and a selection marker, which enables expression of two genes under a single gene regulatory site. Various internal ribosome entry sites are known: for example, those derived from picornavirus, poliovirus, encephalomyocarditis virus, and chicken infectious Fabricius bursal disease virus (cf. Patent Documents 8-10).
Among expression vectors utilizing an internal ribosome entry site, there are known an expression vector in which herpes simplex virus thymidine kinase is incorporated as a selection marker downstream of an internal ribosome entry site (cf. Patent Document 11), and an expression vector in which three or more genes are combined using two or more internal ribosome entry sites (cf. Patent Document 12).
As mentioned above, owing to development of various expression vectors, methods for production of recombinant proteins using mammalian cells have been in practical use for production of medicaments, such as erythropoietin and the like. However, development of expression vectors which are more efficient than conventional ones are consistently sought in order to lower the cost for their production.
The objectives are to provide a novel expression vector for efficient expression of recombinant proteins in mammalian cells, mammalian cells transformed with the vector, and a method for production of such mammalian cells.
In a study directed to the above objectives, the present inventors transformed mammalian cells with an expression vector in which are incorporated a gene expression regulatory site, and a gene encoding a protein of interest, such as human follicle-stimulating hormone, downstream thereof, an internal ribosome entry site further downstream thereof, and a gene encoding glutamine synthetase still further downstream thereof, and a dihydrofolate reductase gene downstream of either the same gene expression regulatory site or another gene expression regulatory site in addition to the former, and thereby found that a high level expression of the gene encoding the protein thereby becomes available, having completed the present invention. Thus, the present invention provides what follows.
1 An expression vector for expression of a protein, comprising a gene expression regulatory site (A), and a gene encoding the protein downstream thereof, an internal ribosome entry site further downstream thereof, a gene encoding a glutamine synthetase still further downstream thereof, and additionally a dihydrofolate reductase gene downstream of either the same gene expression regulatory site (A) or another gene expression regulatory site (B) in addition to the former.
2. The expression vector according to 1 above, wherein the gene expression regulatory site (A) and/or the gene expression regulatory site (B) are selected from the group consisting of a cytomegalovirus-derived promoter, SV40 early promoter, and elongation factor 1 promoter.
3. The expression vector according to 1 or 2 above, wherein the internal ribosome entry site is derived from the 5′ untranslated region of a virus or a gene selected from the group consisting of a virus of Picornaviridae, Picornaviridae Aphthovirus, hepatitis A virus, hepatitis C virus, coronavirus, bovine enterovirus, Theiler's murine encephalomyelitis virus, Coxsackie B virus, human immunoglobulin heavy chain binding protein gene, drosophila antennapedia gene, and drosophila Ultrabithorax gene.
4. The expression vector according to 1 or 2 above, wherein the internal ribosome entry site is derived from the 5′ untranslated region of a virus of Picornaviridae.
5. The expression vector according to 1 or 2 above, wherein the internal ribosome entry site is derived from the 5′ untranslated region of mouse encephalomyocarditis virus.
6. The expression vector according to one of 1 to 5 above, wherein the internal ribosome entry site is that which is prepared by introducing one or more mutations into the nucleotide sequence of a wild-type internal ribosome entry site.
7. The expression vector according to 6 above, wherein the nucleotide sequence of the wild-type internal ribosome entry site includes two or more start codons, wherein part of the two or more codons have been destroyed by the mutation.
8. The expression vector according to 5 above, wherein the internal ribosome entry site comprises the nucleotide sequence set forth as SEQ ID NO:1.
9. The expression vector according to 5 above, wherein the internal ribosome entry site comprises the nucleotide sequence set forth as SEQ ID NO:2.
10. The expression vector according to 5 above, wherein the internal ribosome entry site comprises the nucleotide sequence set forth as SEQ ID NO:3.
11. The expression vector according to 5 above, wherein the internal ribosome entry site comprises the nucleotide sequence set forth as SEQ ID NO:4.
12. The expression vector according to 5 above, wherein the internal ribosome entry site comprises the nucleotide sequence set forth as SEQ ID NO:5.
13. The expression vector according to 5 above, wherein the internal ribosome entry site comprises the nucleotide sequence set forth as SEQ ID NO:6.
14. The expression vector according to one of 1 to 13 above, further comprising, either in the region between the gene encoding the protein and the internal ribosome entry site or in the region downstream of the gene encoding the glutamine synthetase, another internal ribosome entry site in addition to the former internal ribosome entry site, and a drug resistance gene downstream thereof.
15. The expression vector according to one of 1 to 13 above, wherein the expression vector, in addition to the gene expression regulatory site (A) and the gene expression regulatory site (B), further comprises another gene expression regulatory site (C) and a drug resistance gene downstream thereof.
16. The expression vector according to 14 or 15 above, wherein the drug resistance gene is a puromycin or neomycin resistance gene.
17. The expression vector according to one of 1 to 16 above, wherein the gene encoding the protein is a human-derived gene.
18. The expression vector according to 17 above, wherein the human-derived gene is selected from the group consisting of the genes encoding lysosomal enzymes, tissue plasminogen activator (t-PA), blood coagulation factors, erythropoietin, interferon, thrombomodulin, thyroid stimulating hormone (TSH), follicle-stimulating hormone, granulocyte colony-stimulating factor (G-CSF), and antibodies.
19. The expression vector according to 17 above, wherein the human-derived gene is a gene encoding a lysosomal enzyme.
20. The expression vector according to 19 above, wherein the lysosomal enzyme is selected from the group consisting of α-galactosidase A, iduronate-2-sulfatase, glucocerebrosidase, galsulfase, α-L-iduronidase, and acid α-glucosidase.
21. The expression vector according to 17 above, wherein the human-derived gene is a gene encoding erythropoietin.
22. A mammalian cell transformed with the expression vector according to one of 1 to 21 above.
23. The cell according to 22 above, wherein the mammalian cell lacks the intrinsic dihydrofolate reductase gene.
24. The cell according to 22 or 23 above, wherein the mammalian cell is a CHO cell.
25. A method for production of a transformed cell expressing a gene encoding the protein comprising the steps of:
(a) introducing the expression vector according to one of 1 to 21 above into a mammalian cell,
(b) subjecting the mammalian cell containing the introduced expression vector to a selective culture in the presence of an inhibitor of dihydrofolate reductase, and
(c) subjecting the cells selected through the selective culture to a further selective culture in the presence of an inhibitor of glutamine synthetase.
26. A method for production of a transformed cell expressing a gene encoding the protein comprising the steps of:
(a) introducing the expression vector according to one of 14 to 16 above into a mammalian cell,
(b) subjecting the mammalian cell containing the introduced expression vector to a selective culture in the presence of an inhibitor of dihydrofolate reductase, and
(c) subjecting the cells selected through the selective culture to a further selective culture in the presence of an inhibitor of glutamine synthetase,
further comprising the step of subjecting the mammalian cell containing the introduced expression vector to a selective culture in the presence of a drug corresponding to the drug resistance gene.
According to the present invention, an expression vector is provided for efficient expression of a recombinant protein of interest in mammalian cells. Transformed cells which efficiently produce a recombinant protein can be obtained by introducing the expression vector into mammalian cells and then subjecting the cells to a selective culture. Use of thus obtained transformed cells enables significant cost reduction in the production of recombinant proteins.
In the present invention, the term “gene” means structural gene. The term “gene expression regulatory site” means a DNA region which can regulate (control) the transcription frequency of the gene located downstream thereof, and generally is called a promoter or a promoter gene. A gene expression regulatory site is present upstream of almost every gene which is expressed in the body, regulating (controlling) the transcription frequency of the gene, and its nucleotide sequence is diverse. Though there is no particular limitation to it as far as it is able to strongly induce expression of a gene incorporated downstream thereof in mammalian cells, a gene expression regulatory site which can be used in the present invention is preferably a virus-derived promoter, such as a cytomegalovirus (CMV)-derived promoter, SV40 early promoter, and the like; and elongation factor 1α (EF-1) promoter, and the like.
In the present invention, the term “internal ribosome entry site” means a region (structure) inside an mRNA chain to which a ribosome can directly binds and start translation independently from a cap structure, or a region (structure) in a DNA which generates such a region through translation. In the present invention, the term “gene encoding an internal ribosome entry site” means a region (structure) in a DNA which generates such a site through translation. Internal ribosome entry site is generally called IRES, and found in the 5′ untranslated region of viruses of Picornaviridae (poliovirus, rhinovirus, mouse encephalomyocarditis virus, and the like), Picornaviridae Aphthovirus, hepatitis A virus, hepatitis C virus, coronavirus, bovine enterovirus, Theiler's murine encephalomyelitis virus, Coxsackie B virus, and the like, and the 5′ untranslated region of human immunoglobulin heavy chain binding protein, drosophila antennapedia gene, drosophila Ultrabithorax gene, and the like. In the case of a picornavirus, its IRES is a region consisting of about 450 bp present in the 5′ untranslated region of its mRNA. Here, “5′ untranslated region of a virus” means the 5′ untranslated region of a viral mRNA, or a region (structure) in a DNA which, when translated, generates such a region.
In the present invention, there is no particular limitation as to which of internal ribosome entry sites is employed, and any one of them may be used as far as it can act as an internal ribosome entry site in a mammalian cell, in particular a. Chinese hamster ovary-derived cell (CHO cell). Among them, preferred is an internal ribosome entry site derived from the 5′ untranslated region of a virus, more preferred an internal ribosome entry site derived from the 5′ untranslated region of a virus of Picornaviridae, and still more preferred an internal ribosome entry site derived from mouse encephalomyocarditis virus.
In the present invention, internal ribosome entry sites having a wild-type nucleotide sequence may be used directly. Further, any of mutant-type internal ribosome entry sites derived by introducing one or more mutations (such as substitution, deletion, and/or insertion) into one of those wild-type internal ribosome entry sites may also be used so long as it can act as an internal ribosome entry site in mammalian cells (especially, CHO cells). Further, a chimeric-type internal ribosome entry site may also be used which is derived by fusion of two or more internal ribosome entry sites.
In addition, in the present invention, placing a gene encoding a glutamine synthetase (GS gene) under the regulation of an internal ribosome entry site, enables control of expression levels of the GS gene. By controlling the expression levels of the GS gene under the regulation of an internal ribosome entry site, it is possible to select mammalian cells which express a recombinant protein at high levels, as mentioned later.
Besides, in the present invention, a culture performed to select those cells in which a selective marker is incorporated, regardless whether they are GS gene-incorporated cells or not, is referred to as a “selective culture”, and the medium employed to select those cells as a “selective medium”.
Where the expression levels of a GS gene is controlled under the regulation of an internal ribosome entry site, a proper one may be used that is selected as desired from various internal ribosome entry sites. It is also possible to use an internal ribosome entry site that is prepared by incorporating mutations into a wild-type.
For example, where there are multiple start codons (ATG) within a wild-type internal ribosome entry site each of them can be employed as an initiation point of translation, an internal ribosome entry site can be used that is prepared by destruction of part of those start codons. The term “destruction” here means introduction of one or more mutations into a sequence of a gene to thereby prevent the intrinsic function of the gene from working. For example, the internal ribosome entry site of the wild-type mouse encephalomyocarditis virus has three start codons (ATG) at its 3′ end, whose sequence is set forth as SEQ ID NO:1 (5′-ATGataatATGgccacaaccATG-3′: start codons shown in upper letters for clarity). If it is intended to lower the expression level of the GS gene located downstream of this internal ribosome entry site, the start codon to be destroyed by introduction of a mutation is preferably the 2nd or 3rd start codon from the 5′ end, more preferably the 2nd start codon. Thus, examples of an internal ribosome entry sites containing such an introduced mutation include those having at their 3′ end a nucleotide sequence set forth as SEQ ID NO:2 (5′-ATGataatnnngccacaaccnnn-3′: n representing any nucleotide, provided that these three “n” s do not constitute a start codon ATG. The same shall apply hereinafter.) or a nucleotide sequence set forth as SEQ ID NO:7 (5′-ATGataannnngccacaaccnnn-3′) or a nucleotide sequence set forth as SEQ ID NO:3 (5′-ATGataatnnngccacaaccATG-3′) or a nucleotide sequence set forth as SEQ ID NO:8 (5′-ATGataannnngccacaaccATG-3′). More specifically, an example is an internal ribosome entry site having at its 3′ end a nucleotide sequence set forth as SEQ ID NO:4 (5′-ATGataagcttgccacaaccATG-3′), in which the 2nd start codon from the 5′ end has been destroyed by mutation. Still more specifically, the internal ribosome entry site of the wild-type mouse encephalomyocarditis virus comprises a nucleotide sequence set forth as SEQ ID NO:5. Further, an example of nucleotide sequences which are prepared by introducing a mutation into the above nucleotide sequence is the one set forth as SEQ ID NO:6.
Furthermore, the expression levels of a GS gene placed downstream of a wild-type and/or a mutant-type internal ribosome entry site may be controlled by other methods. For example, lowered expression level of the gene can also be achieved either by incorporating the GS gene in an out-of-frame fashion relative to the start codon in the internal ribosome entry site or by introducing a nucleotide sequence that inhibits transcription or translation between the internal ribosome entry site and the GS gene. There is no particular limitation as to a nucleotide sequence that inhibits transcription, and examples include the polymerase addition signal (5′-aataaa-3′) and the like. Examples of such nucleotide sequences that inhibit translation include a stop codon that induces a reading through.
In the present invention, there is no particular limitation as to the term “glutamine synthetase” so long as it is capable of synthesizing glutamine from glutamic acid and ammonia, and it may be of any origin including mammals, reptiles, birds, amphibians, insects such as Bombyx mori, Spodoptera frugiperda, Geometridae, and the like, of Lepidoptera; Drosophila of Diptera; procaryotes; nematodes; yeasts; actinomycetes; filamentous fungi; ascomycetes; Basidiomycota; and plants. Among these, preferred are those originating from mammals, and one originating from human or Chinese hamster (esp. originating from CHO cells) may be preferably used.
In the present invention the term “intrinsic GS gene” means the glutamine synthetase inherently occurring in the original genome of a cell in which an expression vector is to be incorporated, and the term “exogenous GS gene” means a glutamine synthetase gene that is introduced into the cell by an expression vector.
Furthermore, there is no particular limitation as to the term “glutamine synthesis inhibiter”, and any compound may be used so long as it is capable of inhibiting the activity of the glutamine synthetase mentioned above. Preferred examples include methionine sulfoximine (MSX).
If cells in which the expression vector of the present invention has been introduced are cultured in a medium in the presence of an inhibitor of glutamine synthetase (GS inhibitor), cells with lower expression levels of glutamine synthetase will be annihilated because they now are incapable of synthesizing glutamine, and thus those cells with higher expression levels of glutamine synthetase will be selectively obtained. By elevating the concentration of the GS inhibitor stepwise, cells will be obtained which exhibit still higher expression levels of glutamine synthetase. This elevation of the expression levels of glutamine synthetase is brought about mainly through increase in the copy number of the incorporated GS gene in the chromosomes by gene multiplication. When this occurs, the number of the copies of the gene encoding a protein of interest incorporated in the expression vector is also increased by gene multiplication, thus yielding such mammalian cells that express the protein of interest at high levels.
However, CHO cells have an intrinsic GS gene of their own, and therefore there is a possibility that the number of copies of the intrinsic GS gene may be increased through multiplication by a glutamine synthetase inhibitor employed. If this occurred, the cells will grow in the presence of the glutamine synthetase inhibitor even without multiplication of the incorporated GS gene in the expression vector, resulting in failure to obtain cells that exhibit high expression levels of the above-mentioned protein of interest. This problem can be solved by additional incorporation of a second selection marker gene, a different one from a GS gene, into the expression vector.
First, an expression vector containing a GS gene and a second selection marker gene, which is not a GS gene, are incorporated into a cell. The cell, then, is subjected to a selective culture using the second selection marker before performing a selective culture using the GS gene. Since no GS gene receives a selection pressure during the selection process using the second selection marker, the second selection marker incorporated in the expression vector, and therefore the gene encoding the above-mentioned protein of interest and the GS gene incorporated both in the expression vector can be increased in their numbers by multiplication, without receiving any influence from the intrinsic GS gene. As a result, such cells will be obtained that express the above-mentioned protein of interest at levels elevated to a certain degree. Up to this point of the process, the number of copies of the exogenous GS gene has been increased by multiplication, whereas the intrinsic GS gene has never been increased by multiplication.
The selective medium that is employed in the selection using the second selection marker (second selective medium) may be a medium supplemented with a compound, as a selection compound, that is used to perform the selection of cells based on the second selection marker, such as a drug that inhibits the activity of the gene product encoded by the second selection marker, a compound that is cytotoxic and is to be detoxified or attenuated by the gene product encoded by the second selection marker, and the like; or a medium from which such a compound is removed as a selection compound that the cells, after introduction of the second selection marker, will no more require as a nutrient (namely, a compound that is required by the cells as a nutrient only before the introduction of the second marker). In the case where the second selection marker is DHFR, examples of selection compounds to be added to the medium include methotrexate (MTX) and aminopterin. Also if the second selection marker is DHFR, the selection compounds to be removed from the medium are hypoxanthine and thymidine.
If the selection with the second selection marker is performed using a medium supplemented with a selection compound, cells with still further elevated expression levels of the second selection marker can be obtained by stepwise increasing the concentration of the selection compound. This increase in the expression levels of the second selection marker is caused by the increase in the copy number of the second selection marker by gene multiplication, and thus, in this manner, the number of other genes that have been incorporated into the expression vector, i.e., the GS gene and an aforementioned gene encoding a protein of interest, can be increased still more efficiently by multiplication.
Selection of cells then is performed by switching the selection marker to a GS gene. At the start of such a selection using a GS gene as the selection marker, the copy number of the exogenous GS gene has been increased compared with that of the intrinsic GS gene. Therefore, performing a selection of cells by switching the selection marker to a GS gene at this time point will enhance the probability of the exogenous GS gene, which is superior in number, being more greatly amplified by multiplication than the intrinsic GS gene. Thus, by having selected cells in advance by using the second selection marker that is not a GS gene, multiplication of the exogenous GS gene can be boosted during selection using a GS gene while suppressing multiplication of the intrinsic GS gene. As a result, cells that highly express a protein, of interest can be obtained efficiently.
In the present invention, the second selection marker may be incorporated downstream of a separately provided another gene expression regulatory site (the second gene expression regulatory site) in addition to the aforementioned gene expression regulatory site that controls the expression of the recombinant protein of interest. Again, in the present invention, the second selection marker may be provided with a second internal ribosome entry site placed upstream thereof, and through this, be incorporated either into the region between the above-mentioned gene encoding the protein of interest and the internal ribosome entry site downstream thereof, or into the a region downstream of the GS gene. By this way, the expression levels of the second selection marker can be controlled also by the second internal ribosome entry site. In this case, the second internal ribosome entry site may have either the same nucleotide sequence as the internal ribosome entry site upstream of the GS gene or a different nucleotide sequence therefrom. Besides, the second internal ribosome entry site may be chosen as desired from the above-mentioned various internal ribosome entry sites.
In the present invention, while there is no particular limitation as to what is chosen as a second selection marker so far as it is a different selection marker from a GS gene, no gene that confers a drug resistance (drug resistance gene) to mammalian cells is generally not included as candidates of a second selection marker. However, in the case where a third selection marker mentioned later is incorporated into the expression vector to perform further selection of mammalian cells using it, a drug resistance gene may be employed as a second selection marker. A dihydrofolate reductase (DHFR) gene may suitably be used as a second selection marker.
In the present invention, the term “dihydrofolate reductase” (DHFR) means an enzyme that is responsible for the reaction in which dihydrofolate is reduced to tetrahydrofolate. As far as it is an enzyme having this function, there is no particular limitation as for its origin, and thus any one of such enzymes may be employed regardless of their origin including mammals; reptiles; birds; amphibians; insects such as Bombyx mori of Lepidoptera, Spodoptera frugiperda, Geometridae, Drosophila of Diptera; prokaryotes; nematodes; yeasts, Actinobacteria, filamentous fungi, ascomycetes, and basidiomycetes, as well as those originating from plants. Preferred is one originating from mammals, and in particular, those originating from human, mouse, and Chinese hamster (esp. from CHO cells) may suitably be used. In addition to wild-type enzymes originating from these species, mutant-type enzymes that are prepared by introduction of one or more mutations into wild-type enzymes may also be used insofar as they are enzymatically active, and thus are also included in the term “dihydrofolate reductase”. There are such mutant-type enzymes that have, e.g., a difference in their enzyme activity, or a difference in their sensitivity to dihydrofolate reductase inhibitors, from their corresponding wild-type enzymes. Examples of dihydrofolate reductases suitably used include mouse wild-type DHFR, and a mutant-type DHFR that is prepared by replacing the phenylalanine at position 31 from the N-terminus of mouse wild-type DHFR with tryptophan (Mclvor R S et al., Nucleic Acids Research, 18(23), 7025-32 (1990)). The nucleotide sequence and the amino acid sequence of mouse wild-type DHFR are set forth as SEQ ID NO:9 and SEQ ID NO:10, respectively, and the nucleotide sequence and the amino acid sequence of their corresponding mutant-type DHFR are set forth as SEQ ID NO:11 and SEQ ID NO:12.
Further, in the present invention, the term “intrinsic DHFR gene” means the DHFR gene that originally and inherently occurs in the genome of the cells into which an expression vector is to be introduced, and the term “exogenous DHFR gene” means a DHFR gene that is introduced in the cells using an expression vector. Still further, in the present invention, insofar as it can inhibit the activity of the above dihydrofolate reductase (DHFR), there is no particular limitation as to “dihydrofolate reductase inhibitors”, and thus any one of them may be employed, of which preferred are antifolates and more preferred are methotrexate (MTX) and aminopterin.
In the present invention, when cells having an introduced expression vector containing a DHFR gene as a second selection marker are subjected to culture in the presence of a dihydrofolate reductase inhibitor (DHFR inhibitor), those cells which express the lower levels of DHFR will be annihilated because of their failure to synthesize tetrahydrofolate, thereby selectively yielding cells that express DHFR at the higher levels. By increasing stepwise the concentration of the DHFR inhibitor, cells will be obtained that express DHFR at still higher levels. This increase in the expression level of DHFR is mainly due to the increase of the number of copies of the DHFR gene incorporated in the chromosome through gene multiplication. When this occurs, the other gene incorporated in the expression vector, i.e., the GS gene and the gene encoding the protein of interest are also increase in their copy number due to gene multiplication. As a result, cells are obtained that express the protein of interest at some elevated levels and have an increased number of copies of the exogenous GS gene as compared with those of the intrinsic GS gene. At this point of time, by switching the selection marker to the GS gene and carrying out selection of cells, probability of amplification of the exogenous GS gene, which is superior in number, by gene multiplication becomes greater than the intrinsic GS gene. As a result, cells that express the protein of interest can be obtained efficiently.
When increasing stepwise the concentration of a DHFR inhibitor added to a selective medium, its maximum concentration, where the DHFR inhibitor is methotrexate, is preferably 0.25-5 μM more preferably 0.5-1.5 μM, and still more preferably about 1.0 μM.
In the present invention, a third selection marker, in addition to a GS gene and a second selection marker, may be incorporated into the expression vector. A drug resistance gene may suitably be used as a third selection marker. In the present invention, while there is no particular limitation as to what third selection marker may be employed insofar as it can provide a drug resistance to mammalian cells, preferred are genes that can confer the cells with resistance to such drugs as puromycin, hygromycin, blasticidin, and neomycin. With this regard, drugs such as puromycin, hygromycin, blasticidin, neomycin, and the like are “drugs corresponding to the drug resistance genes”, respectively. Among these drug resistance genes, examples include a puromycin resistance gene, a hygromycin resistance gene, a blasticidin resistance gene, and a neomycin resistance gene.
In the present invention, expression levels of a third selection marker may be regulated by incorporating it downstream of a separate gene expression regulatory site (third gene expression regulatory site) provided separately from the gene expression regulatory site by which a recombinant protein is regulated.
Furthermore, in the present invention, a third selection marker may be provided with a second internal ribosome entry site (a third internal ribosome entry site in the case where a second internal ribosome entry site is provided upstream of the second selection maker. The same shall apply hereinafter.) placed upstream thereof, and through this, be incorporated into the region between the gene encoding the protein of interest and the internal ribosome entry site downstream thereof, or into a region downstream of the GS gene, or the like. By this way, the expression level of the third selection marker can be controlled by the second internal ribosome entry site. In this case, the second internal ribosome entry site employed may be either the same as the internal ribosome entry site upstream of the GS gene, and the internal ribosome entry site upstream of the second selection marker if provided, or different one. Further, the second internal ribosome entry site upstream of the third selection marker may be chosen as desired from the various internal ribosome entry sites mentioned above. Also with regard to the second internal ribosome entry site, one or more mutations may be introduced for optimization, as described above.
Where the second selection marker mentioned above is a drug resistance gene, a drug resistance gene used as the third selection marker herein is chosen from drug resistance genes other than the second selection marker. Like the second selection marker, the third selection marker can amplify the GS gene without multiplying the intrinsic GS gene. Thus, by performing selection of cells using the third selection marker, either before or after selection using the second selection marker, the exogenous GS gene can be multiplied without multiplying the intrinsic GS gene. As a result, cells can be efficiently obtained that express the protein of interest at high levels.
In the present invention, there is no particular limitation as to the species of an animal whose gene is incorporated, as encoding a recombinant protein, into an expression vector, whether or not it originates from mammal including human. For example, such a gene is generally of human origin if the expression vector according to the present invention is used for production of ethical pharmaceuticals, and generally originating from the same species of a domestic animal to be treated if the expression vector is used for production of drugs for domestic animals. Again; there is no particular limitation as to what recombinant protein of interest a gene encodes, either, but preferred are such genes that encode lysosomal enzymes including α-galactosidase A, iduronate-2-sulfatase, glucocerebrosidase, galsulfase, α-L-iduronidase, and acid α-glucosidase; tissue plasminogen activator (t-PA); blood coagulation factors including blood coagulation factor WI, blood coagulation factor WIT, and blood coagulation factor IX; erythropoietin, interferons, thrombomodulin, follicle stimulating hormone, granulocyte colony-stimulating factor (G-CSF); DNase I, thyroid stimulating hormone, (TSH) or various antibody medicaments. Examples of antibody medicaments include mouse antibodies, humanized mouse antibodies, and human-mouse chimeric antibodies, human antibodies, and the like, and an example is rituximab, a human-mouse chimeric anti-human CD20 antibody.
In the present invention, where expression of an antibody is intended, a gene encoding either the heavy chain or the light chain of it is incorporated into a vector in such a manner that its expression will be controlled by the gene expression regulatory site that controls the expression of the GS gene, while the gene encoding the other of the chains is incorporated in such a manner that its expression will be controlled by another gene expression regulatory site separately placed in the vector. As gene expression regulatory sites employed here are preferably the same type so that the heavy and light chain of the antibody may be expressed at comparable levels.
Furthermore, in the present invention, if the expression of a protein that forms a heterodimer is intended, a gene encoding one of the subunits taking part in the formation of the heterodimer is incorporated in such a manner that its expression is controlled by the gene expression regulatory site that controls expression of the GS gene, and the gene encoding the other of the subunits is incorporated so that its expression is controlled by another gene expression regulatory site placed in the vector. The expression regulatory sites used for this are preferably of the same type so that the two subunits may be expressed at comparable levels. Examples of proteins that form such heterodimers include follicle stimulating hormone and thyroid stimulating hormone (TSH), and the like.
In the present invention, introduction of an expression vector into mammalian cells is made for the purpose of allowing a gene encoding a recombinant protein to express itself in the mammalian cells. Therefore, it may be made by any method so long as it meets this purpose. An expression vector is a circular plasmid in general, and it may be introduced into cells either in the circular form or after linearized by cleavage with a restriction enzyme.
In the present invention, there is no particular limitation as to mammalian cells into which an expression vector according to the present invention is introduced so long as they can express an intended recombinant protein, and they may be primary culture cells of those collected from organs, muscle tissues, skin tissues, connective tissue, nerve tissue, blood, bone marrow, and the like excised from the body, or their subcultured cells or cell lines established so as to keep their characteristics through repeated subcultures. Those cells may be either normal cells or cells which have become cancerous. Cells which can be used particularly preferably are CHO cells derived from the ovary of a Chinese hamster; human fibroblasts; and COS cells derived from the renal fibroblast of an African green monkey.
Further, mammalian cells into which an expression vector is introduced may have a mutation in their intrinsic gene corresponding to the second selection marker.
In the case where the mutation in the intrinsic gene corresponding to the second selection marker is such that it either lowers or eliminates the expression levels of the gene, or lowers or eliminates the function of the protein encoded by the intrinsic gene, selection pressures from on the selection compound is the more strongly applied to the exogenous second selection marker during the selection process using the second selection marker, and therefore multiplication of the second selection marker is the more accelerated. For example, where the second selection marker is a DHFR gene, if the mutation of the intrinsic DHFR gene is such that it lowers the expression levels of dihydrofolate reductase or eliminates the dihydrofolate reductase, selection pressure from the DHFR inhibitor is applied exclusively to the exogenous DHFR gene during the selection process utilizing the DHFR gene, and therefore multiplication of the exogenous DHFR gene is the more accelerated.
Therefore, by employing such cells that have a mutation in the intrinsic gene corresponding to the second selection marker, cells can be obtained more efficiently that express the protein of interest at high levels.
Before selection by the GS gene, mammalian cells in which the expression vector has been introduced (expression vector-introduced cells) are cultured in a selective medium for cell selection based on the second selection marker. If the second selection marker is DHFR, the medium employed here is a medium supplemented with a DHFR inhibitor. For this, aminopterin or methotrexate (MTX) is preferably used as a DHFR inhibitor. Again, if the second selection marker is a drug resistance gene, the drug corresponding to the drug resistance gene is used.
In the case where DHFR is used as the second selection marker, mammalian cells employed into which an expression vector is to be introduced may be those cells which have a mutation in the intrinsic DHFR gene that causes lowering or elimination of the expression levels of DHFR. Examples of such cells include DG44 strain. DG44 strain fails to express intrinsic DHFR gene. Therefore, when cells of this strain are employed, selection pressure is applied exclusively to the exogenous DHFR gene, and thus the exogenous DHFR gene is efficiently amplified by multiplication. A selective culture may be performed using a medium containing a DHFR inhibitor such as aminopterin or methotrexate (MTX). In doing this, by stepwise increasing the concentration of the DHFR inhibitor, those cells can be obtained which express DHFR at higher levels. Where the concentration of a DHFR inhibitor added to the selective medium is increased stepwise, its maximum concentration, if the DHFR inhibitor is methotrexate, is preferably 0.25-5 μM, more preferably 0.5-1.5 μM, and still more preferably about 1.0 μM.
As a cell strain that lacks a DHFR gene exhibits auxotrophy for hypoxanthine and thymidine, culturing of cells of such a strain to maintain and grow them is conducted in a medium supplemented with these compounds. On the other hand, cells into which an exogenous DHFR gene has been introduced have no auxotrophy for hypoxanthine or thymidine anymore, and therefore, supplementation of the medium with these compounds is not needed. Thus, if an expression vector containing a DHFR gene as a second selection marker is introduced into such cells that exhibit scarce or no DHFR activity to be of an intrinsic DHFR gene due to the lack of the intrinsic DHFR gene, those cells in which the exogenous DHFR gene has been introduced can be selective cultured using a medium containing no hypoxanthine or thymidine as the selective medium. Further, a medium that contains no hypoxanthine or thymidine but is supplemented with a DHFR inhibitor can also be used as a selective medium.
Then, the cells containing the introduced expression vector are cultured in a medium adapted to selection of cells containing an introduced GS gene. A medium employed here is a medium containing little or no glutamine and supplemented with a glutamine synthetase inhibitor (e.g., MSX).
By increasing stepwise the concentration of a GS inhibitor added to the selective medium, cells containing the introduced expression vector that express the GS gene at higher levels can be selected. This is partly due to the fact that the copy number of the GS gene incorporated in the genome of the expression vector-introduced cells multiplies in the process of the selective culture, and among the expression vector-introduced cells, only those with elevated expression levels of the GS gene thus will selectively grow. As the copy number of the gene encoding the recombinant protein incorporated in the expression vector also increases at the same time, the expression levels of the gene also increases. Thus, expression vector-introduced cells with relatively higher expression levels of the recombinant protein of interest can be selected by in this manner of selective culture of the expression vector-introduced cells. In the present specification, expression vector-introduced cells thus selected are referred to as transformed cells.
In the present invention, where the concentration of a GS inhibitor added to a selective medium is increased stepwise, their maximum concentration is preferably 100-1000 μM, more preferably 200-500 μM, and still more preferably about 300 μM where the GS inhibitor is methionine sulfoximine (MSX).
According to the present invention, it is also possible, in addition to selective culture using the second selective medium, to culture the cells containing the introduced expression vector in a medium adapted for selection utilizing a third selection marker before a selective culture using the second selective medium. In this case, the third selective culture using the third selection marker may be carried out either before or after the second selective culture. The third selection marker is a drug resistance gene. Therefore, a drug corresponding to the drug resistance gene is added to the selective medium. In doing this, increasing stepwise the concentration of the drug in the selective medium will give cells expressing the drug resistance gene at increased levels. When increasing stepwise the concentration of the drug added to the selective medium that corresponds to the drug resistance gene, the maximum concentration of the drug is preferably 3-30 μM if the drug is puromycin, and more preferably 5-20 μM, still more preferably about 10 μM. If the drug is G418, its maximum concentration is 0.4-1.5 mg/mL, more preferably 0.8-1.2 mg/mL, and still more preferably about 1 mg/mL.
Though the present invention will be described in further detail below with reference to examples, it is not intended that the present invention be limited to the examples.
[Construction of pE-Neo Vector and pE-Hygr Vector]
pEF/myc/nuc vector (Invitrogen) was digested with KpnI and NcoI to cut out a region which includes EF-1 promoter and its first intron, which then was blunt-ended with T4 DNA polymerase. Separately, pCI-neo (Invitrogen), after digested with BgIII and EcoRI to remove a region containing CMV enhancer/promoter and introns, was blunt-ended with T4 DNA polymerase. Into this was inserted the above-mentioned region (after blunt-ended) including above-mentioned EF-1α promoter and its first intron to construct pE-neo vector (
pE-neo vector was digested with SfiI and BstXI to cut out a region of about 1 kbp including a neomycin resistance gene (
[Construction of pE-IRES-GS-Puro]
An expression vector pPGKIH (Miyahara M. et. al., J. Biol. Chem. 275, 613-618(2000)) was digested with restriction enzymes (XhoI and BamHI) to cut out a DNA fragment consisting of a nucleotide sequence IRES-Hygr-mPGKpA, which included an internal ribosome entry site (IRES) derived from mouse encephalomyocarditis virus (EMCV), a hygromycin resistance gene (Hygr gene), and the polyadenylation region (mPGKpA) of mouse phosphoglycerate kinase (mPGK)(SEQ ID NO:15; from the 5′ end, the region consisting of nucleotides 1-6 represents a “XhoI site”; the region consisting of nucleotides 120-715 and nucleotides 716-718 (atg) that follow represents a “nucleotide sequence including the internal ribosome entry site derived from the 5′ untranslated region of mouse encephalomyocarditis virus”; the region consisting of nucleotides 716-1741 including in itself the nucleotides 716-718 (atg) represents the “nucleotide sequence encoding a hygromycin resistance gene”; the region consisting of nucleotides 1747-2210 represents a “nucleotide sequence including the polyadenylation region of mouse phosphoglycerate kinase”; and the region at the 3′ end consisting of six nucleotides (nucleotides 2211-2216) represents a “BamHI site”). (Besides, the amino acid sequence corresponding to the Hygrr gene is set forth as SEQ ID NO:16). This DNA fragment was inserted into pBluescript SK(−)(Stratagene) between its XhoI and BamHI sites, and the resulting product was designated pBSK(IRES-Hygr-mPGKpA)(
A DNA fragment containing part of the IRES of EMCV was amplified by PCR using pBSK (IRES-Hygr-mPGKpA), as a template, and primer IRES5′ (SEQ ID NO:17) and primer IRES3′ (SEQ ID NO:18). This fragment then was digested with restriction enzymes (XhoI) and HindIII) and inserted into pBSK(IRES-Hygr-mPGKpA) between its XhoI and HindIII sites, and the resulting product was designated pBSK(NotI-IRES-Hygr-mPGKpA) (
Using the expression vector pPGKIH, as a template, and primer mPGKP5′ (SEQ ID NO:19) and primer mPGKP3′ (SEQ ID NO:20), PCR was performed to amplify a DNA fragment consisting of a nucleotide sequence including the promoter region of mPGK (mPGKp)(SEQ ID NO:21, from the 5′ end, nucleotides 4-9 represents a “BgIII site”, the region that follows consisting of nucleotides 10-516 represents a “nucleotide sequence including the promoter region of mouse phosphoglycerate kinase (mPGKp)”, and the region that follows consisting of nucleotides 524-529 represents an “EcoRI site”). This DNA fragment then was digested with restriction enzymes (BgIII and EcoRI) and inserted into pCI-neo (Promega) between its BgIII and EcoRI sites, and the resulting product was designated pPGK-neo (
cDNA was prepared from CHO-K1 cells, and using it, as a template, and primer GS5′ (SEQ ID NO:22) and primer GS3′ (SEQ ID NO:23), PCR was performed to amplify a DNA fragment including the GS gene. The DNA fragment was digested with restriction enzymes (Ball and BamHI) and inserted into pPGK-IRES-Hygr between its Ball and BamHI sites. The resulting product was designated pPGK-IRES-GS-ΔpolyA (
Using pCAGIPuro (Miyahara M. et. al., J. Biol. Chem. 275, 613-618 (2000)), as a template, and primer puro5′ (SEQ ID NO:24) and primer puro3′ (SEQ ID NO:25), PCR was performed to amplify a nucleotide sequence including a puromycin resistance gene (puror gene) (SEQ ID NO:26, from the 5′-end, the region consisting of nucleotides 2-7 represents a “AfIII” site, the region that follows consisting of nucleotides 8-607 represents a “nucleotide sequence encoding the puromycin resistance gene (puror gene)”, and the region that follows consisting of nucleotides 608-619 represents a “BstXI site”) (Besides, the amino acid sequence corresponding to the puror gene is set forth as SEQ ID NO:27). This DNA fragment was digested with restriction enzymes (AfIII and BstXI) and inserted into the expression vector pE-neo between its AfIII and BstXI sites. The resulting product was designated pE-puro (
Using pE-puro, as a template, and primer SV40polyA5′ (SEQ ID NO:28) and primer SV40polyA3′ (SEQ ID NO:29), PCR was performed to amplify a DNA fragment including SV40 late polyadenylation region. This DNA fragment then was digested with restriction enzymes (NotI and HpaI) and inserted into pE-puro between its NotI and HpaI sites. The resulting product was designated pE-puro(XhoI) (
[Construction of pE-mIRES-GS-Puro]
Using the expression vector pE-IRES-GS-puro, as a template, and primer mIRES-GS5′ (SEQ ID NO:30) and primer mIRES-GS3′ (SEQ ID NO:31), PCR was performed to amplify a region from the IRES to GS of EMCV, and thus a DNA fragment was amplified in which the second start codon (ATG) from the 5′ end of the IRES of EMCV was destroyed by introduction of a mutation. Using the expression vector pE-IRES-GS-puro, as a template, and the DNA fragment and the above-mentioned primer IRES5′, PCR was performed to amplify a DNA fragment including a region from IRES to GS. This DNA fragment was digested with restriction enzymes (NotI and PstI), and a DNA fragment thus cut out was inserted into the expression vector pE-IRES-GS-puro between its NotI and PstI sites. The resulting product was designated pE-mIRES-GS-puro (
[Construction of pE-mIRES-GS]
Using the expression vector pE-neo, as a template, and primer SV40polyA5′-2 (SEQ ID NO:32) and primer SV40polyA3′-2 (SEQ ID NO:33), PCR was performed to amplify a DNA fragment including the SV40 late polyA region. This DNA fragment was digested with restriction enzymes (XhoI and BamHI) and inserted into pE-mIRES-GS-puro between its XhoI and BamHI sites. The resulting product was designated pE-mIRES-GS (
[Construction of Human Thyroid Stimulating Hormone (hTSH) Expression Vector]
[Construction of pE-mIRES-GS-mNeo]
Using pCI-neo (Invitrogen), as a template, and primer mNeoA5′ (SEQ ID NO:34) and primer mNeoA3′ (SEQ ID NO:35), PCR was performed to amplify the full length of this plasmid, and through self-ligation, plasmid pCI-mNeo containing E182D mutant-type neomycin resistance gene (mNeor gene) was constructed. Using the pCI-mNeo vector, as a template, and primer mNeoB5′ (SEQ ID NO:36) and primer mNeoB3′ (SEQ ID NO:37), PCR was performed to amplify a DNA fragment containing mNeor gene. This DNA fragment was digested with a restriction enzyme (EagI) and inserted into pUC57-IE1 (Gene Scrip) at its NotI site. The resulting product was designated pUC57-IE1-mNeo. Using pCI-neo, as a template, and primer mNeoC5′ (SEQ ID NO:38) and primer mNeoC3′ (SEQ ID NO:39), PCR was performed to amplify a region containing a 3′ region of the neomycin resistance gene (neon gene) and the synthetic poly A signal, and the product was used as a megaprimer (neo-polyA). Then, using pUC57-1E1-mNeo, as a template, and primer mNeoD5′ (SEQ ID NO:40) and the megaprimer (neo-polyA), PCR was performed to amplify a region containing the mNeor gene and the synthetic poly A signal. The DNA fragment thus obtained was digested with restriction enzymes (AfIII and BamHI), and inserted into pE-mIRES-GS-puro between its Anil and BamHI sites. The product thus obtained was designated pE-mIRES-GS-mNeo (
[Construction of pBlue-EF1/mIRES-mNeo]
pE-mIRES-GS-mNeo was digested with a restriction enzyme (EcoRI) to cut out a DNA fragment containing the elongation factor 1 promoter (EF-1p), the mutant-type internal ribosome entry site (mIRES) and part of the glutamine synthetase (GS). This DNA fragment was inserted into pBluescript SK(+) (Stratagene) at its EcoRI site. The product thus obtained was designated pBlue-EF1/mIRES (
pE-mIRES-GS-mNeo was digested with a restriction enzyme (PvuI) to cut out a DNA fragment containing the mutant-type neomycin resistance gene (mNeor gene) and a DNA fragment containing the SV40 late polyA region. Using the DNA fragment containing the SV40 late polyA region, as a template, and primer SVpA-Mega-F (SEQ ID NO:41) and primer SVpA-BstXI-R (SEQ ID NO:42), PCR was performed to amplify the same DNA fragment containing the SV40 polyA region. By ligating this gene fragment and the above-mentioned DNA fragment containing the mNeor gene, a DNA fragment was prepared which contained SV40 late polyA region downstream of the mNeor gene. This DNA fragment was digested with a restriction enzyme (BstXI), and inserted into pBlue-EF1/mIRES between its BstXI sites. The product thus obtained was designated pBlue-EF1/mIRES-mNeo (
[Construction of pBlue-EF1/SVpA]
Using the expression vector pE-mIRES-GS-mNeo, as a template, and primer SVpA-Not-F (SEQ ID NO:43) and primer SVpA-BstXI-R (SEQ ID NO:44), PCR was performed to amplify a DNA fragment containing the SV40 late polyA region. This DNA fragment was digested with restriction enzymes (NotI and BstXI), and inserted into pBlue-EF1/mIRES between its NotI and BstXI sites. The product thus obtained was designated pBlue-EF1/SVpA (
[Construction of pE-mDHFR31]
A DNA (SEQ ID NO:45) was synthesized that contained a mutant-type DHFR (F31W) gene (mDHFR31)(SEQ ID NO:11), a gene in which phenylalanine at position 31 from the N-terminus of the mouse wild-type DHFR has been replaced with tryptophan. This DNA was inserted into pGEM-T easy vector (Promega) at its 3′-T overhang portion, and the resulting product was designated pGEM(mDHFR31). pGEM(mDHFR31) then was digested with restriction enzymes (AfIII and BstXI) to cut out a DNA fragment containing the mutant-type DHFR(F31W), and this DNA fragment was inserted into pE-neo between its AfIII and BstXI sites. The product thus obtained was designated pE-mDHFR31 (
[Construction of pCI-Neo(hTSHα-WAP3′UTR) and pCI-Neo(hTSHβ-WAP3′UTR)]
A DNA containing a gene for the α chain of human thyroid stimulating hormone (hTSHα) (SEQ ID NO:46, in which, from the 5′ end, the region consisting of nucleotides 1-6 represents a “MluI site”, the region consisting of nucleotides 14-364 represents the “nucleotide sequence encoding hTSHα chain”, and the region that follows consisting of nucleotides 365-372 represents a “NotI” site) and a DNA containing a gene for the β chain of human thyroid stimulating hormone (hTSHβ) (SEQ ID NO:47, in which, from the 5′ end, the region consisting of nucleotides 1-6 represents a “MluI site”, the region consisting of nucleotides 14-433 represents the “nucleotide sequence encoding TSHβ chain”, and the region that follows consisting of nucleotides 434-441 represents a “NotI site”) were synthesized, and they were respectively inserted into pUC57 to prepare pUC57-hTSHα, and pUC57-hTSHβ (Shanghai ShineGene Molecular Biotech). Separately, a DNA (SEQ ID NO:48) was synthesized containing the 3′ untranslated region of rabbit whey acidic protein (WAP), and then was incorporated into pUC57 to prepare pUC57-WAP3′UTR (Shanghai ShineGene Molecular Biotech).
Using pUC57-hTSHα containing hTSHα gene, as a template, and primer TSHα5′ (SEQ ID NO:49) and primer TSHα3′ (SEQ ID NO:50), PCR was performed to amplify a DNA fragment containing TSHα gene, and the DNA fragment thus obtained was used as a megaprimer TSHα. Separately, using a DNA containing hTSHβ gene, as a template, and primer TSH (35′ (SEQ ID NO:51) and primer TSH (33′ (SEQ ID NO:52), PCR was performed to amplify a DNA fragment containing TSHβ gene. This DNA fragment thus obtained was used as a megaprimer TSHβ.
Using pUC57-WAP3′UTR containing WAP3′UTR, as a template, and the megaprimer TSHα and primer WAP3′ (SEQ ID NO:53), PCR was performed to amplify a DNA fragment containing the TSHα gene and WAP3′UTR downstream of it (hTSHα-WAP3′UTR). Separately, using pUC57-WAP3′UTR containing WAP3′UTR, as a template, and the megaprimer TSHβ and primer WAP3′, PCR was performed to amplify a DNA fragment containing TSHβ gene and WAP3′UTR downstream of it (hTSHβ-WAP3′UTR). Each of the fragments thus obtained was digested with restriction enzymes (MluI and NotI), and inserted into pCI-neo between its MluI and NotI sites to prepare pCI-neo(hTSHα-WAP3′UTR) and pCI-neo(hTSHβ-WAP3′UTR) (
[Construction of pE-mIRES-GS-mNeo(hTSHβ)] pCI-neo(hTSHβ-WAP3′UTR) was digested with restriction enzymes (MluI and NotI) to cut out a DNA fragment containing the hTSHβ gene and WAP3′UTR. This DNA fragment was inserted into pE-mIRES-GS-mNeo between its MluI and NotI sites. The product thus obtained was designated pE-mIRES-GS-mNeo(hTSHβ) (
[Construction of pBlue-EF1/mIRES-mNeo(hTSHα)]
pCI-neo(hTSHα-WAP3′UTR) was digested with restriction enzymes (MluI and NotI) to cut out a DNA fragment containing hTSHα gene and WAP3′UTR. This DNA fragment was inserted into pBlue-EF1/mIRES-mNeo between its MluI and NotI sites. The product thus obtained was designated pBlue-EF1/mIRES-mNeo(hTSHα) (
[Construction of pE-mIRES-GS-mNeo-dual(hTSH)]
pBlue-EF1/mIRES-mNeo(hTSHα) was digested with restriction enzymes (ClaI and BgIII) to cut out a DNA fragment containing the elongation factor 1 promoter (EF-1p), hTSHα gene, mIRES, and the mutant-type neomycin resistance gene (mNeor). This DNA fragment was inserted into pE-mIRES-GS-mNeo(hTSHβ) between its ClaI and BamHI sites. The product thus obtained was designated pE-mIRES-GS-mNeo-dual(hTSH), a vector for expression of human thyroid stimulating hormone (hTSH) (
[Construction of pE-mIRES-GS-mNeo-Dual+mDHFR31 (hTSH)]
Using pE-mDHFR31, as a template, and primer dE-BbsI-F (SEQ ID NO:54) and primer SynpA-BbsI-R (SEQ ID NO:55), PCR was performed to amplify a DNA fragment containing SV40 early promoter (SV40 enhancer/promoter), mutant-type DHFR (F31W), and a synthetic polyadenylation signal (a synthesized region for polyadenylation). The DNA fragment thus obtained was digested with a restriction enzyme (BbsI) and inserted into pE-mIRES-GS-mNeo-dual(hTSH) between its BsmBI sites. The product thus obtained was designated pE-mIRES-GS-mNeo-dual+mDHFR31(hTSH), a vector for expression of human thyroid stimulating hormone (hTSH) (
[Construction of pE-mIRES-GS-mNeo(RTX-H)]
A DNA containing the gene for the heavy chain of rituximab (SEQ ID NO:56; from the 5′ end, the region consisting of nucleotides 1-6 represents a “MluI site”, the region consisting of nucleotides 18-1430 represents the “nucleotide sequence encoding the heavy chain of rituximab”, and the region that follows consisting of nucleotide 1431-1438 represents a “NotI site”) was synthesized. This DNA was digested with restriction enzymes (MluI and Nod), and the DNA fragment thus obtained was inserted into pE-mIRES-GS-mNeo between its MluI and NotI sites. The product thus obtained was designated pE-mIRES-GS-mNeo(RTX-H) (
[Construction of pBlue-EF1/mIRES-mNeo(RTX-L)]
A DNA containing the gene for the light chain of rituximab (SEQ ID NO:57; from the 5′ end, the region consisting of nucleotides 1-6 represents a “MluI site”, the region consisting of nucleotides 18-725 represents the “nucleotide sequence encoding the light chain of rituximab”, and the region that follows consisting of nucleotides 726-733 represents a “NotI site”) was synthesized. This DNA was digested with restriction enzymes (MluI and NotI), and the DNA fragment thus obtained was inserted into pBlue-EF1/mIRES-mNeo between its MluI and NotI sites. The product thus obtained was designated pBlue-EF1/mIRES-mNeo(RTX-L) (
[Construction of pE-mIRES-GS-mNeo-Dual(RTX)]
pBlue-EF1/mIRES-mNeo(RTX-L) was digested with restriction enzymes (ClaI and BgIII), and the DNA fragment obtained was inserted into pE-mIRES-GS-mNeo(RTX-H) between ClaI and BgIII sites. The product thus obtained was designated pE-mIRES-GS-mNeo-dual(RTX), a vector for expression of rituximab (
[Construction of pE-mIRES-GS-dual+mDHFR31(RTX)]
A synthetic DNA containing the DNA set forth as SEQ ID NO:57 which contained the DNA for the light chain of rituximab was digested with restriction enzymes (MluI and NotI), and the DNA fragment thus obtained was inserted into pBlue-EF1/SVpA between its MluI and NotI sites. The product thus obtained was designated pBlue-EF1/SVpA(RTX-L) (
pBlue-EF1/SVpA(RTX-L) was digested with restriction enzymes (ClaI and BgIII), and the DNA fragment thus obtained was inserted into pE-mIRES-GS-mNeo(RTX-H) between its ClaI and BamHI sites. The product thus obtained was designated pE-mIRES-GS-dual(RTX) (
Using pE-mDHFR31, as a template, and primer dE-BbsI-F (SEQ ID NO:58) and primer SynpA-BbsI-R (SEQ ID NO:59), PCR was performed to amplify a DNA fragment containing SV40 early promoter (SV40 enhancer/promoter), the mutant-type DHFR (F31W), and the synthetic polyadenylation signal. This DNA fragment was digested with a restriction enzyme (BbsI) and inserted into pE-mIRES-GS-dual(RTX) between its BsmBI sites. The product thus obtained was designated pE-mIRES-GS-dual+mDHFR31(RTX), a vector for expression of rituximab (
[Production of hTSH Expressing Cells and Rituximab Expressing Cells]
By electroporation (Invitrogen), CHO-K1 cells, which was cells derived from a Chinese hamster ovary cell, were transformed with pE-mIRES-GS-mNeo-dual(hTSH), pE-mIRES-GS-mNeo-dual+mDHFR31(hTSH), pE-mIRES-GS-mNeo-dual(RTX), and pE-mIRES-GS-dual+mDHFR31(RTX), respectively. The cells thus transformed were selective cultured in selective mediums, and hTSH expressing cells and RTX expressing cells were respectively obtained.
In the selective culture of cells that had been transformed with pE-mIRES-GS-mNeo-dual+mDHFR31(TSH) and pE-mIRES-GS-dual+mDHFR31(RTX), the cells were at first cultured in CD Opti CHO medium (Invitrogen) supplemented with methotrexate (Wako), with stepwise increasing the concentration of methotrexate in the medium, and finally in the medium containing 0.5 μM methotrexate, to allow cells exhibiting resistance to methotrexate to grow selectively. Then, the medium was replaced with a CD Opti CHO medium (Invitrogen) supplemented with methionine sulfoximine (Sigma), and the cells were cultured with stepwise increase of the concentration of methionine sulfoximine, and finally in the medium containing 300 μM methionine sulfoximine, to allow cells exhibiting resistance to methionine sulfoximine to grow selectively. The cells thus obtained were treated as bulk cells.
In selective culture of cells transformed with pE-mIRES-GS-mNeo-dual(TSH) and E-mIRES-GS-mNeo-dual(RTX), the cells were cultured in CD Opti CHO medium (Invitrogen) supplemented with methionine sulfoximine and G418, with stepwise increasing the concentration of methotrexate and G418 in the medium, and finally in the medium containing 300 μM methionine sulfoximine and 1 mg/mL G418, to allow cells exhibiting resistance to both of the drugs to grow selectively. The cells thus obtained were treated as bulk cells.
The respective bulk cells obtained by the selective culture described above were seeded to a 96-well plate so that 1-3 cells were to be contained per well, and cultured for about two weeks in CD Opti CHO medium (Invitrogen) until colonies formed. Through measurement of the concentration of hTSH and RTX in the culture supernatant, cells with high expression levels were collected. In doing this, methionine sulfoximine was added to the medium at the concentration of 300 μM. These cloned cells were used in the following experiments as TSH expressing cells and RTX expressing cells.
[Measurement of Expression Levels of hTSH in hTSH Expressing Cells]
The TSH expressing cells obtained through the selective culture were cultured at a cell density of 2×105 cells/mL in 5 mL of CD Opti CHO medium containing 300 μM methionine sulfoximine for 10 days at 37° C., 5% CO2. The culture supernatant was sampled on days 3, 7, and 10 of the culture.
A solution of mouse anti-hTSH-beta monoclonal antibody (Leinco Technologies) was added 100 μL each to a 96-well plate, and the plate was left undisturbed for one hour at room temperature to let the antibody adhere to the plate. The solution was discarded, and after the plate was washed three times with TBS-T solution (Tris: 0.05M, NaCl: 0.138 M, KCl: 0.0027 M, 0.05% Tween20, pH 8.0), 1% BSA/TBS-T solution was added 200 μL each to the plate, and the plate was left undisturbed for one hour at room temperature for blocking. The solution was discarded and after the plate was washed three times with TBS-T solution, the sampled culture supernatant was added to the plate 100 μL each as a test sample, and the plate was left undisturbed for one hour at room temperature. In doing this, as needed, the culture supernatant was diluted with TBS-T solution. Further, a solution prepared by diluting hTSH (Nibsc) to 0.244-62.5 ng/mL with TBS-T solution was added as a standard solution to the plate in the same manner as the test samples, and the plate was left undisturbed. The solution was discarded, and after the plate was washed three times with TBS-T solution, HRP-labeled mouse anti-hTSH-alpha monoclonal antibody (Leinco Technologies) was added 100 μL each to the plate as the secondary antibody, and the plate was left undisturbed for one hour at room temperature. The plate was washed three times with TBS-T solution, and after addition of HRP substrate (Wako), the plate was left undisturbed for 15 minutes at room temperature, and sulfuric acid then was added to stop the reaction. Absorption was measured at 490 nm on a microwell plate reader, and the values for test samples were interposed into the standard curve produced from the measurements of the standard solution to determine the concentration of hTSH contained in the test sample.
The RTX expressing cells obtained through the selective culture were cultured at a cell density of 2×105 cells/mL in 5 mL of CD Opti CHO medium containing 300 μM methionine sulfoximine for 10 days at 37° C., 5% CO2. The culture supernatant was sampled on days 3, 7, and 10 of the culture.
Expression levels of rituximab were measured using Human IgG ELISA Quantitation Set (Bethyl Laboratories). Solution of goat anti-hIgG antibody (Bethyl Laboratories) was added 100 μL each to a 96-well plate, and the plate was left undisturbed for one hour at room temperature to let the antibody adhere to the plate. The solution was discarded, and after the plate was washed three times with TBS-T solution, 1% BSA/TBS-T solution was added 200 μL each, and the plate was left undisturbed for one hour at room temperature for blocking. The solution was discarded, and after the plate was washed three times with TBS-T solution, the sampled culture supernatant was added to the plate 100 μL each as a test sample, and the plate was left undisturbed for one hour at room temperature. In doing this, as needed, the culture supernatant was diluted with TBS-T solution. Further, a solution prepared by diluting rituximab (Chugai Pharmaceutical) to 3.9-500 ng/mL with TBS-T solution was added as a standard solution to the plate in the same manner as the test samples, and the plate was left undisturbed. The solution was discarded, and after the plate was washed three times with TBS-T solution, HRP-labeled goat anti-hIgG antibody (Bethyl Laboratories) was added 100 μL each to the plate as the secondary antibody, and the plate was left undisturbed for one hour at room temperature. The plate was washed three times with TBS-T solution, and after addition of HRP substrate (Wako), the plate was left undisturbed for 15 minutes at room temperature, and sulfuric acid then was added to the plate to stop the reaction. Absorption was measured at 490 nm on a microwell plate reader, and the values for test samples were interposed into the standard curve produced from the measurements of the standard solution to determine the concentration of rituximab contained in the test sample.
[Results (Expression Levels of hTSH in hTSH Expressing Cells)]
With the CHO cells transformed with the human thyroid stimulating hormone (hTSH) expression vector pE-mIRES-GS-mNeo-dual(hTSH), the concentration of hTSH in the medium on day 10 after starting the culture was 22.0 μg/mL for the bulk cells, and 29.2 μg/mL for the cloned cells. On the other hand, with the CHO cells transformed with pE-mIRES-GS-mNeo-dual+mDHFR31(hTSH), an expression vector containing incorporated DHFR gene as the second selection marker, the concentration of hTSH in the medium on day 10 after starting the culture was 35.3 μg/mL for the bulk cells, and 85.2 μg/mL for the cloned cells (Table 1).
Namely, compared with the case of the hTSH expression vector pE-mIRES-GS-mNeo-dual(hTSH) that contains elongation factor 1 promoter(EF-1p), hTSH gene, mutant-type internal ribosome entry site (mIRES), and GS gene as the first selection marker in this order, the CHO cells transformed with pE-mIRES-GS-mNeo-dual+mDHFR31(hTSH), which was a hTSH expression vector containing additionally incorporated DHFR gene as the second selection marker, exhibited strikingly increased expression levels of hTSH, i.e., about 1.6 times between respective bulk cells, and about 2.9 times between respective cloned cells.
With the CHO cells transformed with the RTX expression vector pE-mIRES-GS-mNeo-dual(RTX), the concentration of rituximab in the medium on day 10 after starting the culture was 188.6 μg/mL for the bulk cells, and 318.9 μg/mL for the cloned cells. On the other hand, with the CHO cells transformed with pE-mIRES-GS-dual+mDHFR31(RTX), an expression vector containing incorporated DHFR gene as the second selection marker, the concentration of rituximab in the medium on day 10 after the start of the culture was 174.1 μg/mL for the bulk cells, and 401.5 μg/mL for the cloned cells (Table 2).
Namely, compared with the case of the RTX expression vector pE-mIRES-GS-mNeo-dual(RTX) that contains elongation factor 1 promoter (EF-1p), rituximab gene, mutant-type internal ribosome entry site (mIRES), and GS gene as the first selection marker in this order, the cells transformed with pE-mIRES-GS-dual+mDHFR31(RTX), an RTX expression vector containing additionally incorporated DHFR gene as the second selection marker, exhibited levels of RTX expression which, while almost the same between respective bulk cells, was found increases about 1.26 times between respective cloned cells.
The above results indicates that an expression vector that contains, downstream of a gene expression regulatory site, an exogenous gene, a mutant-type internal ribosome entry site, and a glutamine synthetase as a first selection marker, incorporated in this order, and an additionally incorporated dihydrofolate reductase gene as a second selection marker, functions as an expression vector that enables expression of the exogenous gene at high levels.
As the present invention enables expression of a recombinant protein at high levels using mammalian cells, it can be utilized to realize a great reduction of cost for production of products containing a recombinant protein, such as ethical drugs containing a recombinant protein.
SEQ ID NO:2: Modified murine encephalomyocarditis virus; n is a, c, g, or t
SEQ ID NO:3: Modified murine encephalomyocarditis virus; n is a, c, g, or t
SEQ ID NO:4: Modified murine encephalomyocarditis virus
SEQ ID NO:6: Modified murine encephalomyocarditis virus
SEQ ID NO:7: Modified murine encephalomyocarditis virus; n is a, c, g, or t
SEQ ID NO:8: Modified murine encephalomyocarditis virus; n is a, c, g, or t
SEQ ID NO:11: Modified Mus musculus DHFR
SEQ ID NO:13: Primer Hyg-Sfi5′, synthetic sequence
SEQ ID NO:14: Primer Hyg-BstX3′, synthetic sequence
SEQ ID NO:15: IRES-Hygr-mPGKpA, synthetic sequence
SEQ ID NO:17: Primer IRES5′, synthetic sequence
SEQ ID NO:18: Primer IRES3′, synthetic sequence
SEQ ID NO:19: Primer mPGKP5′, synthetic sequence
SEQ ID NO:20: Primer mPGKP3′, synthetic sequence
SEQ ID NO:21: mPGKp, synthetic sequence
SEQ ID NO:22: Primer GS5′, synthetic sequence
SEQ ID NO:23: Primer GS3′, synthetic sequence
SEQ ID NO:24: Primer puro5′, synthetic sequence
SEQ ID NO:25: Primer puro3′, synthetic sequence
SEQ ID NO:26: Synthetic sequence containing puromycin resistance gene
SEQ ID NO:28: Primer SV40polyA5′, synthetic sequence
SEQ ID NO:29: Primer SV40polyA3′, synthetic sequence
SEQ ID NO:30: Primer mIRES-GS5′, synthetic sequence
SEQ ID NO:31: Primer mIRES-GS3′, synthetic sequence
SEQ ID NO:32: Primer SV40polyA5′-2, synthetic sequence
SEQ ID NO:33: Primer SV40polyA3′-2, synthetic sequence
SEQ ID NO:34: Primer mNeoA5′, synthetic sequence
SEQ ID NO:35: Primer mNeoA3′, synthetic sequence
SEQ ID NO:36: Primer mNeoB5′, synthetic sequence
SEQ ID NO:37: Primer mNeoB3′, synthetic sequence
SEQ ID NO:38: Primer mNeoC5′, synthetic sequence
SEQ ID NO:39: Primer mNeoC3′, synthetic sequence
SEQ ID NO:40: Primer mNeoD5′, synthetic sequence
SEQ ID NO:41: Primer SVpA-Mega-F, synthetic sequence
SEQ ID NO:42: Primer SVpA-BstXI-R, synthetic sequence
SEQ ID NO:43: Primer SVpA-Not-F, synthetic sequence
SEQ ID NO:44: Primer SVpA-BstXI-R, synthetic sequence
SEQ ID NO:45: Synthetic sequence containing Modified DHFR(F31W)
SEQ ID NO:46: Synthetic sequence containing human TSHα
SEQ ID NO:47: Synthetic sequence containing human TSHβ
SEQ ID NO:48: Synthetic sequence containing WAP3′UTR
SEQ ID NO:49: Primer TSHα5′, synthetic sequence
SEQ ID NO:50: Primer TSHα3′, synthetic sequence
SEQ ID NO:51: Primer TSHβ5′, synthetic sequence
SEQ ID NO:52: Primer TSHβ3′, synthetic sequence
SEQ ID NO:53: Primer WAP3′, synthetic sequence
SEQ ID NO:54: Primer dE-BbsI-F, synthetic sequence
SEQ ID NO:55: Primer SynpA-BbsI-R, synthetic sequence
SEQ ID NO:56: Rituximab heavy chain, synthetic sequence
SEQ ID NO:57: Rituximab light chain, synthetic sequence
SEQ ID NO:58: Primer dE-BbsI-F, synthetic sequence
SEQ ID NO:59: Primer SynpA-BbsI-R, synthetic sequence
GP167-PCT ST25
| Number | Date | Country | Kind |
|---|---|---|---|
| 2012-102796 | Apr 2012 | JP | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/JP2013/062251 | 4/25/2013 | WO | 00 |
