Claims
- 1. An isolated protein that is pesticidal wherein
a. said protein has toxin activity against a lepidopteran pest, and wherein said protein comprises an amino acid sequence coded for by a nucleotide sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:12, and SEQ ID NO:15; or b. said protein has toxin activity against a corn rootworm pest, and wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:44, and SEQ ID NO:46; or c. said protein has toxin activity against a corn rootworm pest, and wherein said protein comprises an amino acid sequence coded for by a nucleotide sequence selected from the group consisting of SEQ ID NO:30, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:144.
- 2. An isolated polynucleotide that encodes a protein wherein
a. said protein has toxin activity against a lepidopteran pest, wherein said protein comprises an amino acid sequence coded for by a nucleotide sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:12, and SEQ ID NO:15; or b. said protein has toxin activity against a corn rootworm pest, wherein said polynucleotide hybridizes under conditions of 0.1×SSPE at 65° C. with the full complement of a nucleotide sequence selected from the group consisting of SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:45, SEQ ID NO:48, SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:144.
- 3. A recombinant host comprising an isolated polynucleotide that encodes a protein, wherein said host is selected from the group consisting of a plant cell, a microbial cell, and a plant, and wherein
a. said protein has toxin activity against a lepidopteran pest, and wherein said protein comprises an amino acid sequence coded for by a nucleotide sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:12, and SEQ ID NO:15, or b. said protein has toxin activity against a corn rootworm pest, wherein said polynucleotide hybridizes under conditions of 0.1×SSPE at 65° C. with the full complement of a nucleotide sequence selected from the group consisting of SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:45, SEQ ID NO:48, SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:144.
- 4. A method of controlling a pest wherein said method comprises administering an isolated protein to said pest wherein
a. said pest is a lepidopteran pest, and wherein said protein comprises an amino acid sequence coded for by a nucleotide sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:12, and SEQ ID NO:15; or b. said pest is a corn rootworm pest, and wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:44, and SEQ ID NO:46; or c. said pest is a corn rootworm pest, and wherein said protein comprises an amino acid sequence coded for by a nucleotide sequence selected from the group consisting of SEQ ID NO:30, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:144.
- 5. A purified culture of a Bacillus thuringiensis isolate selected from the group consisting of Bacillus thuringiensis isolates PS10E1, PS31F2, PS33D2, PS66D3, PS68F, PS69AA2, PS146D, PS168G1, PS175I4, PS177C8a, PS177I8, PS185AA2, PS196J4, PS196F3, PS197T1, PS197U2, PS202E1, PS217U2, KB33, KB38, KB53A49-4, KB68B46-2, KB68B51-2, and KB68B55-2, and mutants thereof.
- 6. The protein of claim 1 wherein said protein said protein comprises an amino acid sequence coded for by the nucleotide sequence of SEQ ID NO:10.
- 7. The protein of claim 1 wherein has toxin activity against a lepidopteran pest, and wherein said protein comprises an amino acid sequence coded for by the nucleotide sequence of SEQ ID NO:12, and said protein further comprises an amino acid sequence coded for by the nucleotide sequence of SEQ ID NO:15.
- 8. The polynucleotide of claim 2 wherein said polynucleotide encodes a protein that has toxin activity against a lepidopteran pest, wherein said protein comprises an amino acid sequence coded for by the nucleotide sequence of SEQ ID NO:10.
- 9. The polynucleotide of claim 2 wherein said polynucleotide encodes a protein that has toxin activity against a lepidopteran pest, wherein said protein comprises an amino acid sequence coded for by the nucleotide sequence of SEQ ID NO:12, and said protein further comprises an amino acid sequence coded for by the nucleotide sequence of SEQ ID NO:15.
- 10. The host according to claim 3 wherein said protein has toxin activity against a lepidopteran pest, and wherein said protein comprises an amino acid sequence coded for by the nucleotide sequence of SEQ ID NO:10.
- 11. The host according to claim 3 wherein said protein has toxin activity against a lepidopteran pest, and wherein said protein comprises an amino acid sequence coded for by the nucleotide sequence of SEQ ID NO:12, and said protein further comprises an amino acid sequence coded for by the nucleotide sequence of SEQ ID NO:15.
- 12. The host according to claim 3 where said host is a plant cell.
- 13. The host according to claim 3 where said host is a bacterial cell.
- 14. The host according to claim 3 where said host is a plant.
- 15. The method of claim 4 wherein said pest is a lepidopteran pest, and wherein said protein comprises an amino acid sequence coded for by a nucleotide sequence of SEQ ID NO:10.
- 16. The method of claim 4 wherein said pest is a lepidopteran pest, and wherein said protein comprises an amino acid sequence coded for by a nucleotide sequence selected from the group consisting of SEQ ID NO:12, and said protein further comprises an amino acid sequence coded for by the nucleotide sequence of SEQ ID NO:15.
- 17. The protein of claim 1 wherein said protein has toxin activity against a corn rootworm pest, and wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:44, and SEQ ID NO:46.
- 18. The protein of claim 1 wherein said protein has toxin activity against a corn rootworm pest, and wherein said protein comprises an amino acid sequence coded for by a nucleotide sequence selected from the group consisting of SEQ ID NO:30, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:144.
- 19. The polynucleotide of claim 2 wherein said protein has toxin activity against a corn rootworm pest, wherein said polynucleotide hybridizes under conditions of 0.1×SSPE at 65° C. with the full complement of a nucleotide sequence selected from the group consisting of SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:45, SEQ ID NO:48, SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:144.
- 20. The recombinant host of claim 3 wherein said protein has toxin activity against a corn rootworm pest, wherein said polynucleotide hybridizes under conditions of 0.1×SSPE at 65° C. with the full complement of a nucleotide sequence selected from the group consisting of SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:35, SEQ ID NO:41, SEQ ID NO:45, SEQ ID NO:48, SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:144.
- 21. The method of claim 4 wherein said pest is a corn rootworm pest, and wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:44, and SEQ ID NO:46.
- 22. The method of claim 4 wherein said pest is a corn rootworm pest, and wherein said protein comprises an amino acid sequence coded for by a nucleotide sequence selected from the group consisting of SEQ ID NO:30, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:141, SEQ ID NO:142, and SEQ ID NO:144.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of co-pending application Ser. No. 09/073,898, filed May 6, 1998; which is a continuation-in-part of Ser. No. 08/960,780, filed Oct. 30, 1997, now U.S. Pat. No. 6,204,435; which claims priority from provisional application Ser. No. 60/029,848, filed Oct. 30, 1996.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60029848 |
Oct 1996 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09073898 |
May 1998 |
US |
Child |
09850351 |
May 2001 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
08960780 |
Oct 1997 |
US |
Child |
09073898 |
May 1998 |
US |