Claims
- 1-29. (canceled).
- 30. A purified polypeptide molecule comprising the amino acid sequence SEQ ID NO: 5 or SEQ ID NO: 5 with conservative amino acid substitutions.
- 31. An antibody that binds specifically to AatA.
- 32. A method of detecting the presence of AatA in a sample comprising the steps of:
contacting said sample with the antibody of claim 31; and detecting the binding of said antibody to AatA; wherein said binding is indicative of the presence of AatA in said sample.
- 33. The method of claim 32, wherein said presence of AatA is indicative of the presence of enteroaggregative E. coli.
- 34-54. (canceled).
- 55. An immunogenic composition capable of stimulating an immune response in an animal to enteroaggregative E. coli comprising a pharmaceutically acceptable carrier and one or more of the isolated, purified proteins selected from the group consisting of Aat, AatP, AatA, AatB, AatC, and AatD or fragments thereof.
- 56. The immunogenic composition of claim 55, wherein said immunogenic composition is administered by a route selected from the group consisting of oral administration, nasal administration, inhalation, ophthalmic administration, rectal administration and vaginal administration.
- 57. A method of generating an immune response to enteroaggregative E. coli in an animal comprising administering the immunogenic composition of claim 55 to the animal.
- 58-60. (canceled).
- 61. A kit comprising:
a container, the immunogenic composition of claim 55 contained within said container, and instructions for using the immunogenic composition to elicit an immune response to enteroaggregative E. coli; wherein said label on said container indicates that said immunogenic composition can be used to elicit an immune response in an animal to enteroaggregative E. coli.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This non-provisional application claims priority under 35 U.S.C. §120 to provisional applications U.S. Serial Nos. 60/334,425 and 60/398,775, filed Nov. 30, 2001 and Jul. 26, 2002, respectively, the contents of which are incorporated herein by reference in their entirety.
STATEMENT OF GOVERNMENTAL INTEREST
[0002] This invention was made with U.S. Government support under NIADA grant No. AI33096 awarded by the National Institutes of Health. The U.S. Government may have certain rights in this invention.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60334425 |
Nov 2001 |
US |
|
60398775 |
Jul 2002 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
10307294 |
Dec 2002 |
US |
Child |
10847321 |
May 2004 |
US |