Patent Application
20120064527
The present invention relates to a nucleic acid analysis device and a nucleic acid analysis apparatus, for example.
A sequencing method in a nucleic acid analysis apparatus has been recently proposed, which comprises immobilizing many DNA probes or polymerases on a reaction device prepared using a glass substrate or the like and then performing a base extension reaction on the reaction device. A region in which such immobilization and reaction are performed is hereinafter referred to as “reaction spot.”
A single molecule is immobilized (single molecule scheme) or a plurality of molecules of the same type are immobilized (multimolecular scheme) at a reaction spot. Also, a massively parallel nucleic acid analysis apparatus has also been developed, whereby many reaction spots are disposed and base extension and sequencing are performed in parallel in many reaction spots.
Non-patent document 1 explains a case in which a single molecule is immobilized at a reaction spot. Non-patent document 1 describes DNA sequencing using a total reflection evanescent irradiation detection system at the single molecule level. Specifically, a laser having an excitation wavelength of 532 nm and a laser having an excitation wavelength of 635 nm are used as excitation light for exciting fluorescence from fluorophore Cy3 and fluorophore Cy5, respectively. First, a single target DNA molecule is immobilized on the solution layer side on a refractive index boundary plane using biotin-avidin protein binding, forming a reaction spot. Cy3-labeled primers are introduced into a solution by solution exchange, so that the single fluorescence-labeled primer molecule hybridizes to the target DNA molecule. The hybridization reaction is performed for a certain time period, and then excessive primers that have remained unreacted are washed off. Subsequently, as a result of total reflection evanescent irradiation using excitation light (532 nm), since Cy3 is present in the evanescent field, the position of binding of the target DNA molecule can be confirmed by fluorescence detection. After the confirmation, Cy3 is irradiated with high-power excitation light for fluorescence photobleaching, thereby suppressing the subsequent fluorescent emission.
Next, polymerase and a single type of base labeled with Cy5, dNTP (N denotes A, C, G, or T), are introduced into a solution by solution exchange, the fluorescence-labeled dNTP molecule is incorporated into the extension chain of the primer molecule only when it is complementary to the target DNA molecule. The extension reaction is performed for a certain time period, and then excessive dNTP that has remained unreacted is washed off. Subsequently, as a result of total reflection evanescent irradiation using excitation light (635 nm), since Cy5 is present in the evanescent field, the complementary relationship can be confirmed by fluorescence detection at the binding position of the target DNA molecule. After confirmation, Cy5 is irradiated with high-power excitation light for fluorescence photobleaching, so as to suppress the subsequent fluorescent emission. In the above reaction process for incorporation of dNTP, a base sequence that is complementary to the target DNA molecule can be determined through stepwise extension reaction; that is, the repetition of the sequential use of base types, such as A→C→G→T→A→ . . . .
A plurality of reaction spots are formed within regions (hereinafter referred to as “visual measurement field(s)”) that can be observed simultaneously by a detector to be used for fluorescent measurement, and then the above reaction processes for dNTP incorporation are performed in parallel while different target DNA molecules are present in reaction spots. This enables simultaneous DNA sequencing for a plurality of target DNA molecules. It is expected that the number of subjects that can be simultaneously treated in parallel can be drastically increased compared with conventional DNA sequencing based on electrophoresis.
Also, a single molecule DNA sequencer does not require gene amplification by a PCR or the like, because of its mechanism. However, when a target DNA fragment to be observed is rare or only a single DNA fragment, a single molecule DNA sequencer can ideally read the target without wasting the DNA fragment.
Furthermore, there is a method in advanced research that uses a combination of semiconductor chips having microstructures for generation of plasmon resonance or the like in order to perform DNA sequencing (determination of the base sequence) for a single molecular unit. For example, Patent document 1 describes the use of the effects of enhancing fluorescence to a degree about several to dozens of times that of localized surface plasmons. The effect of enhancing fluorescence can reach the range of about 10 nm to 20 nm. When localized surface plasmons are generated on the surface of a metal microstructure to which a target DNA molecule has been immobilized, only the fluorescence-labeled dNTP incorporated into the target DNA molecule receives the benefit from the enhanced fluorescence, resulting in a difference in fluorescence intensity several to dozens of times or more greater than that for floating fluorescence-labeled dNTP. Such a scheme makes it possible to measure a base extension reaction without removing unreacted fluorescence-labeled dNTP.
Also, various methods have been proposed whereby target molecules are aligned in arbitrary shapes or at arbitrary positions. Non-patent document 2 proposes a method that involves firstly providing an electrode in a desired pattern on a substrate, coating the entire substrate surface with PLL-g-PEG (Poly-L-Lysin-g-polyethylene glycol), and applying voltage to the electrode, so as to remove PLL-g-PEG on the electrode part, and thus causing fluorescent molecules or the like to specifically adsorb to the electrode part alone. Non-patent document 3 describes a technique that involves coating a substrate with photodissociative molecules and then preparing an immobilization region pattern for nanoscale target molecules by a lithography technique using near-field scanning light. According to these techniques, a pattern of 100-nm or less DNAs or proteins is prepared on a substrate.
Meanwhile, Non-patent document 4 discloses real-time DNA sequencing analysis that involves supplying different fluorescent dyes to 4 types of nucleotide and causing serial nucleic acid extension reactions without washing. Also, Patent document 2 discloses a method for controlling the topical initiation of a base extension reaction, which involves disposing a protecting group cleavable by light irradiation at position 3′ of a probe. Specifically, a caged compound is disposed as a protecting group at position 3′ on the oligo probe, the protecting group is cleaved by UV irradiation, and then a real-time base extension reaction is initiated.
Patent Documents
Non-Patent Documents
The present inventors have obtained the following findings as a result of intensive studies to improve the throughput of a massively parallel nucleic acid analysis apparatus.
In the case of the massively parallel nucleic acid analysis apparatus, the throughput is improved proportionally to regions (specifically, the number of effective reaction spots within measurement regions) that can be measured simultaneously with a single optical detection system composed of a lens and a detector. Also, reaction spots are disposed in high density on a reaction device, so that the amount of a reagent to be used herein is reduced since the reaction chamber size becomes smaller even with the same number of reaction spots, and analysis with lower cost becomes possible. However, in reality, the number of simultaneously measurable effective reaction spots and the density of reaction spots in a reaction device are limited by the resolution of the optical detection system and the number of pixels of the detector.
The resolution of an optical detection system is determined on the basis of the diffraction limit of an objective lens composing the optical detection system. The diffraction limit is specifically determined according to the following equation.
Diffraction limit=0.61×λ/NA [Equation 1]
(where “λ” denotes the wavelength of light to be measured and “NA” denotes the numerical aperture of an objective lens.)
The wavelength of fluorescence to be measured ranges from about 500 nm to 800 nm, while the NA of an objective lens is about 1. According to the above equation, the diffraction limit of an objective lens ranges from about 300 nm to 500 nm. The resolution of an actual optical detection system is even lower than the above values because of the aberration, positional precision, and the like for a lens, such as about 1 μm. Accordingly, reaction spots must be at a distance of about 1 μm or more away from each other in order to ensure the identification of fluorescence on individual reaction spots. On the other hand, the range of the measurable visual field (effective visual field size) depends on the NA of an objective lens to be used. When the NA is about 1, the effective visual field size is about 1 mm2. Hence, reaction spots should be formed with a pitch of 1 μm within a 1-mm2 range in order to maximize the number of reaction spots within a measurement region. At this time, the maximum number of reaction spots is about 1×106.
An even larger number of reaction spots should be formed to further improve the throughput. Hence, there is a method that involves forming 1×106 or more reaction spots on a substrate and measuring while scanning.
When the above measurement is performed, the signal intensity should be suppressed within the dynamic range of a detector. Accordingly, excitation light intensity within the measurement region should be as uniform as possible. Thus, the excitation light irradiation region should be larger than the measurement region. Excitation light also leaks to reaction spots adjacent to a measurement region to be subjected to fluorescence measurement, so that light leakage occurs. In this case, a fluorescent dye is decomposed via irradiation with excitation light and quenching takes place, and quenching of fluorescence may be caused within the reaction spots adjacent to the measurement region to be subjected to fluorescence measurement. When the adjacent reaction spots are within unmeasured regions, in the case of a multimolecular scheme, some of fluorophores labeled with a plurality of molecules of the same type within the reaction spots or fluorophores labeled with molecules to be incorporated into a plurality of molecules of the same type are quenched by light leakage and thus sufficient signal intensity may not be obtained. This increases the noise information against the base sequence to be determined. In the case of the single molecule scheme, only one target molecule is present within a reaction spot, and the problem of quenching due to light leakage is more serious than in the multimolecular scheme.
A possible means for addressing the problem of fluorescence quenching due to light leakage is to conduct measurement for regions sufficiently distant from each other so that individual irradiation regions are not allowed to overlap each other. However, when a structure wherein measurement regions are sufficiently distant from each other is employed, target molecules within reaction spots existing between the measurement regions are not measured, and the information or target molecules existing in the region cannot be obtained.
In view of the above circumstances, an object of the present invention is to reduce reaction spot waste on a nucleic acid analysis device in a nucleic acid analysis apparatus, and to provide a nucleic acid analysis device by which the leakage of fluorescence excitation light to unobserved measurement regions is suppressed.
As a result of intensive studies to achieve the above object, the present inventors have found that the leakage of fluorescence excitation light to regions other than the target nucleic acid measurement region can be suppressed by disposing one nucleic acid measurement region so that it is at a sufficient distance from other nucleic acid measurement regions on a nucleic acid analysis device, and so that other nucleic acid measurement regions do not enter the irradiation region. Thus, the present inventors have completed the present invention.
Specifically, the present invention relates to a nucleic acid analysis device having a plurality of nucleic acid measurement regions wherein one nucleic acid measurement region is disposed so that it is at a sufficient distance from other nucleic acid measurement regions, and so that other nucleic acid measurement regions do not enter the irradiation region. Also, the present invention relates to a nucleic acid analysis apparatus comprising the nucleic acid analysis device and a nucleic acid analysis method using the nucleic acid analysis device.
This description includes part or all of the content as disclosed in the description and/or drawings of Japanese Patent Application No. 2009-127907, which is a priority document of the present application.
The present invention exerts an effect of reliably capturing fluorescence signals from target nucleic acids immobilized within the target nucleic acid measurement regions.
The nucleic acid analysis device according to an embodiment is a reaction device having a plurality of nucleic acid measurement regions, wherein one nucleic acid measurement region is disposed so that it is at a sufficient distance from the other nucleic acid measurement regions so that the other nucleic acid measurement regions do not enter an irradiation region. In other words, it can be said that the nucleic acid analysis device is characterized by having a plurality of nucleic acid measurement regions, and blank portions that have no reaction spot between the nucleic acid measurement regions and by its illumination of one nucleic acid measurement region with a light source. Through nucleic acid analysis using the nucleic acid analysis device according to an embodiment, since unreacted nucleic acid measurement regions are not principally irradiated with excitation light, noise information against a base sequence to be determined can be reduced and fluorescence signals from individual target nucleic acids immobilized in reaction spots within a target nucleic acid measurement region can be reliably observed. Also, the nucleic acid analysis device according to an embodiment can be installed in an analysis apparatus such as a nucleic acid analysis apparatus and used for genetic testing, for example.
Here the term “nucleic acid measurement region(s)” refers to a region(s) having one or a plurality of reaction spots in which a target nucleic acid such as a target DNA molecule is immobilized and a reaction for nucleic acid analysis is performed.
The nucleic acid analysis device according to an embodiment is produced by providing nucleic acid measurement region(s) on a substrate. Examples of substrates are not particularly limited and include substrates made of material such as quartz or silicon.
Regarding a nucleic acid measurement region(s), only one nucleic acid measurement region is provided within an irradiation region to be irradiated with excitation light, and nucleic acid measurement regions are disposed on the substrate so that they are at a sufficient distance from each other so that the other nucleic acid measurement regions do not enter the irradiation region. A blank portion having no reaction spot is present between the nucleic acid measurement regions. When image acquisition is performed using a highly sensitive camera with a ½-inch two-dimensional image sensor of a currently available CCD or CMOS imaging device and an about ×40 objective lens, about a 140 μm square (that is, about a 20000-μm2 region) can be observed. This suggests that when the size of a single visual field is an about a 140 μm square, pixels of an imaging device will never go to waste. The size of a nucleic acid measurement region in the nucleic acid analysis device according to an embodiment is preferably substantially the same as that of the visual measurement field of such an optical detection system. Therefore, the size (long side or greatest dimension) of a nucleic acid measurement region on a substrate ranges from 50 μm square to 10 mm square, and it is particularly preferably 140 μm square, for example. Also, examples of the shapes of nucleic acid measurement regions include squares, quadrangles, and circles.
Meanwhile, a visual measurement field of the above size (that is, an about a 140 μm square) is irradiated uniformly with a laser beam. Moreover, a nucleic acid measurement region is disposed so as to avoid the effects of excitation light in neighboring visual fields. For these purposes, the intervals between adjacent nucleic acid measurement regions are determined in view of laser beam irradiation distribution, ranging from 1 μm to 10 mm and preferably ranging from 50 μm to 200 μm, for example. In particular, the intervals between nucleic acid measurement regions are desirably determined to have a value corresponding to single visual field (that is, about 140 μm). Such a width is determined in view of the intensity uniformity within a laser irradiation region to be used herein and desired excitation light intensity. For example, when a single nucleic acid measurement region is illuminated with a laser as light for illumination, the laser diameter and the dimension of a blank portion are limited to dimensions that allow neighboring nucleic acid measurement regions to avoid being irradiated with light that leaks. Alternatively, a nucleic acid measurement region group consisting of a predetermined number of nucleic acid measurement regions can also be irradiated with a light source.
Meanwhile, all reaction spots of a nucleic acid measurement region to be observed may be contained within a light irradiation region to which illumination intensity required for nucleic acid analysis is applied with light for illumination such as a laser, for example. Alternatively, all light irradiation regions to which illumination intensity required for measurement is applied may be contained within a nucleic acid measurement region to be observed.
When a laser is used as light for illumination, only a predetermined visual observation field (visual measurement field) can be irradiated with a laser homogenizer as explained in the following Embodiment 4.
Nucleic acid measurement regions are disposed in every direction in a grid on a substrate, such that about 10 nucleic acid measurement regions are disposed vertically and about 10 nucleic acid measurement regions are disposed horizontally. In addition, the number of nucleic acid measurement regions on a substrate is preferably determined in view of the throughput for observation of a reaction and the number of exchange of nucleic acid analysis devices according to an embodiment per nucleic acid analysis, so that the properties and usability of a nucleic acid analysis apparatus can be improved to the highest degree. Also, nucleic acid measurement regions may be disposed on a reagent flow channel, as explained in the following Embodiment 2.
In nucleic acid measurement regions, reaction spots are present. The number of reaction spots in one nucleic acid measurement region ranges from 100 to 108 and preferably ranges from 104 to 106.
In a nucleic acid measurement region, a target nucleic acid is immobilized on reaction spots. Examples of a target nucleic acid include DNA, RNA, and PNA (peptide nucleic acid). Examples of a method for immobilization of a target nucleic acid onto a reaction spot include methods using antigen-antibody binding, binding of a tag with a substance binding thereto such as a His-Tag (histidine tag)/nitrilotriacetic acid (NTA) or iminodiacetic acid (IDA) and GST-Tag (glutathione S-transferase tag)/glutathione, avidin-biotin binding, or the like. For example, a target nucleic acid is specifically immobilized on reaction spots using biotin-avidin binding (biotin is bound to either a reaction spot or a target nucleic acid, and avidin is bound to the other). Also, an adsorption-preventing molecule is immobilized at regions other than the nucleic acid measurement region on a substrate so as to prevent unnecessary adhesion of the target nucleic acid. An example of such an adsorption-preventing molecule is, but is not particularly limited to, PLL-g-PEG described in Non-patent document 2. For example, according to the method described in Non-patent document 2, an electrode of a desired pattern is provided on a substrate, PLL-g-PEG is applied to the entire substrate surface, voltage is applied to the electrode so as to remove PLL-g-PEG on the electrode part, and then a target nucleic acid is immobilized within the region from which PLL-g-PEG has been removed. Alternatively, according to the method of using the lithography technique using near-field scanning light described in Non-patent document 3, for example, a target nucleic acid can be immobilized on reaction spots.
Furthermore, when a metal structure is used as described in Patent document 1, the metal structure is formed only within nucleic acid measurement region(s). Specifically, for example, in the case of gold, a target nucleic acid can be immobilized on a metal structure via gold-thiol binding. As described above, through immobilization of a target nucleic acid on a metal structure, fluorescence from a fluorescent molecule incorporated into the target nucleic acid to be detected upon nucleic acid analysis can be enhanced. Also, when such a metal structure is made of a rare metal, reaction spots in the nucleic acid analysis device according to an embodiment can be efficiently used. Thus, the consumption of such a rare metal can be further reduced compared with a conventional nucleic acid analysis device.
Also, for example, a nucleic acid analysis device can have a nucleic acid probe having a photodegradable substance that inhibits a nucleic acid extension reaction and a reaction field region (nucleic acid measurement region) in which a plurality of the nucleic acid probes are disposed. As explained in the following Embodiment 4, a photodegradable substance (protecting group that can be cleaved by light irradiation) is bound to a nucleic acid probe, the substance is cleaved by UV irradiation, and thus a base extension reaction is initiated. With the use of this method, a base extension reaction is inhibited at a stage at which no UV irradiation is performed, and the reaction can be initiated by UV irradiation. Examples of a photodegradable substance include caged compounds such as a 2-nitrobenzyl type, a decyl phenacyl type, or a coumarynylmethyl type (Patent document 2). The term “caged compound” is a generic name for a bioactive molecule that has been modified with a photodegradable protecting group so as to tentatively lose its activity. The generic term, “caged compound(s)” refers to a molecule(s) with bioactivity that is caged and caused to sleep.
To conduct nucleic acid analysis, a nucleic acid analysis apparatus is provided with a nucleic acid analysis device produced as described above. The apparatus can comprise, in addition to a nucleic acid analysis device, a means for supplying fluorescence-labeled primers, dNTP (N denotes A, C, G, or T), and the like to a nucleic acid analysis device, a means for irradiating the nucleic acid analysis device with light, a light emission detection means for measuring fluorescence of fluorescent molecules (with which a primer or dNTP is labeled) resulting from hybridization to a target nucleic acid or a nucleic acid extension reaction on the nucleic acid analysis device. Furthermore, the apparatus has reaction solution flow channels and a solution delivering mechanism capable of delivering a solution to a predetermined nucleic acid measurement region of the nucleic acid analysis device.
Base sequence information on a target nucleic acid can be obtained by the nucleic acid analysis apparatus according to an embodiment. For example, a solution containing a primer labeled with a fluorescent molecule is supplied to a nucleic acid measurement region on a nucleic acid analysis device. Subsequently, the fluorescent molecule is incorporated into the target nucleic acid as a result of hybridization of the target nucleic acid with the primer. The nucleic acid measurement region is irradiated with excitation light suitable for the fluorescent molecule with which the primer is labeled, fluorescence is detected, and thus the hybridization can be confirmed. Furthermore, a solution containing dNTP that is labeled with a fluorescent molecule having fluorescence properties (fluorescence wavelength or excitation wavelength) differing from those of the fluorescent molecule with which polymerase (e.g., DNA polymerase, RNA-dependent DNA polymerase (reverse transcriptase), RNA polymerase, and RNA-dependent RNA polymerase) and a primer are labeled is supplied to a nucleic acid measurement region. Thus, a base extension reaction takes place. Subsequently, the nucleic acid measurement region is irradiated with excitation light suitable for the fluorescent molecule with which dNTP is labeled, and then fluorescence is detected. Based on the fluorescence, the base information of the target nucleic acid can be obtained.
Also, with the use of the nucleic acid analysis device according to an embodiment, all base extension reactions on reaction spots can be completely observed. The nucleic acid analysis device according to an embodiment can be applied to single molecule DNA sequencing wherein a rare or the only one DNA fragment is a target nucleic acid. Furthermore, with the use of the nucleic acid analysis device according to an embodiment, a base extension reaction of the target nucleic acid is performed with a real-time scheme, and thus base sequence information can also be obtained.
Hereinafter, preferred embodiments for implementing the present invention are described with reference to the attached drawings. Here, each embodiment is an example of typical embodiments of products or methods relating to the present invention, and is not intended to limit the scope of the present invention.
In this embodiment, an example of a nucleic acid analysis device and an example of an optical detection system in a single molecule nucleic acid analysis apparatus to which plasmon resonance is applied are explained as follows.
Also,
In the apparatus shown in
Also, when a base extension reaction is allowed to proceed on the nucleic acid analysis device, fluorescence incorporated into the target DNA molecule immobilized on the metal structure 102 can be measured. The fluorescence is captured as a two-dimensional image by an optical detection system consisting of a fluorescence wavelength filter 108 (that is, an optical filter that allows the transmission of only fluorescence wavelength), an imaging lens 107, and a detector 106.
The present embodiment is most significantly characterized in that the metal structures 102 are separately disposed within each nucleic acid measurement region 109. The nucleic acid measurement regions 109 are disposed at intervals so as not to affect the other nucleic acid measurement regions when a specific nucleic acid measurement region is illuminated within the excitation light irradiation region 105. In the embodiment, nucleic acid measurement regions are disposed at intervals of 300 μm in the laser irradiation direction and at intervals of 100 μm in the direction perpendicular to the laser irradiation. In addition, the distance is determined such that the irradiation intensity distribution of a laser to be used herein is sufficient for excitation of a fluorescent dye to be observed and the intervals are maintained so as not to affect neighboring visual measurement fields.
In the apparatus shown in
Next,
As explained above, with the use of a scheme for subsequently measuring each nucleic acid measurement region using a nucleic acid analysis device, irradiation with light for illumination is possible only upon measurement while excluding metal structures within immeasurable regions.
Moreover, through repetition of shifting and measurement, all nucleic acid measurement regions on the nucleic acid analysis device 101 are measured. At the stage at which all measurements are completed, measurement of single base extension is completed. Thereafter, the dNTP type within a primer is changed subsequently in order of A, C, G, and then T. Solutions containing such primers are each subjected to a nucleic acid analysis device. Every time such a solution is subjected to the device, measurement and shifting of all nucleic acid measurement regions are repeated, so as to cause a base extension reaction to proceed and to determine the base sequence of a target DNA molecule.
In this embodiment, measurement of a plurality of types of sample is explained.
A nucleic acid analysis device 401 with reagent flow channels shown in
The nucleic acid measurement regions 404 within the reagent flow channels 403 are subjected to treatment of surfaces to which a target DNA molecule is adsorbed, according to the method for accelerating specific adsorption as described in Non-patent document 2 or 3 above (specifically, a method using PLL-g-PEG). Alternatively, regions other than the nucleic acid measurement regions 404 may be treated by techniques for chemical, photochemical, or electromagnetic non-specific adsorption prevention treatment or physical substrate surface modification treatment, so as to prevent the adsorption of the target DNA molecule.
In the present embodiment, a reagent containing a target DNA molecule having a linker for specific adsorption is injected from the inlet ports 402 into the nucleic acid analysis device 401. The target DNA molecule is specifically adsorbed to only the nucleic acid measurement regions 404 as a result of the surface treatment. After a sufficient amount of the target DNA molecule is immobilized, a washing liquid is injected from the inlet ports 402 and then the reagent is discharged. Furthermore, a primer labeled with a fluorescent molecule is introduced from the inlet ports 402 to a certain concentration via solution exchange, the single fluorescence-labeled primer molecule hybridizes to only the target DNA molecule complementary to the primer molecule. After hybridization is performed sufficiently, a washing liquid is injected from the inlet ports 402 and then the primer is discharged.
Next, each nucleic acid measurement region is irradiated with excitation light 406 and then fluorescence is measured. After completion of the measurement, excitation light is irradiated to a degree such that fluorescence is sufficiently photobleached, and thus quenching of fluorescence is caused to take place within the measurement region. At the stage at which measurement of all measurement regions is completed, measurement for single base extension is completed. Thereafter, the dNTP type within a primer is changed subsequently in order of A, C, G, and then T. Solutions containing such primers are each subjected to a nucleic acid analysis device. Every time such a solution is subjected to the device, measurement and shift of all nucleic acid measurement regions are repeated, so as to cause a base extension reaction to proceed and to determine the base sequence of a target DNA molecule.
The present embodiment wherein the nucleic acid analysis device has reagent flow channels makes it possible to analyze a plurality of different samples (that differ by reagent flow channels) using reagent flow channels (e.g., reagent flow channel 403/reagent flow channel 407/reagent flow channel 408/reagent flow channel 409) as shown in
Furthermore, the present embodiment is characterized in that when a reaction that requires preparation and/or aftertreatment is performed, such treatments can be performed using unobserved reagent flow channels.
First, in step 1, primer injection into the reagent flow channel 403 is performed. Neither measurement nor photobleaching can be performed during primer injection. After completion of primer injection, measurement in visual measurement field 1 is initiated as step 2. On the other hand, measurement or photobleaching is not performed in the reagent flow channel 407, primer injection can be performed independently from the reagent flow channel 403.
Furthermore, in step 3, while visual measurement field 2 is subjected to measurement in the reagent flow channel 403, photobleaching is performed in visual measurement field 1, during which primer injection can be continued in the reagent flow channel 407. As described above, steps 2-7 are performed for the reagent flow channel 403, and primer injection can be performed in the reagent flow channel 407 in parallel with these steps. After completion of the measurement of visual measurement field 6 in the reagent flow channel 403, as step 8, photobleaching is subsequently performed in visual measurement field 6 of the reagent flow channel 403, simultaneously with measurement in visual measurement field 1 of the reagent flow channel 407. Therefore, all visual measurement fields corresponding to a single base extension in the reagent flow channel 403 are completed.
On and after step 9, injection of a primer containing the next dNTP type is initiated, during which measurement and photobleaching can be performed in the reagent flow channel 407 as step 9 to step 12.
With the above steps, the time required for primer injection can be shortened and base sequences can be determined with even higher throughput.
In the present embodiment, another example of the disposition of nucleic acid measurement regions in the nucleic acid analysis device is as described below.
In an optical system for measuring a reaction in square or rectangular nucleic acid measurement regions, the resolution of the peripheral part may be insufficient depending on the performance of the optical system. In such a case, circular nucleic acid measurement regions are employed, observation is made for sites other than the peripheral site where the performance is decreased, and thus observation results with even higher quality may be obtained.
In the present embodiment, as shown in
According to the nucleic acid analysis device shown in
The present embodiment is an embodiment using a real-time extension reaction system, wherein in the single molecule nucleic acid analysis apparatus shown in Embodiment 1, dNTP molecules are continuously incorporated into the extending chain of the primer molecule. In real-time DNA sequencing analysis described in Non-patent document 4, four types of nucleotide having different fluorescent dyes are supplied, so as to cause successive nucleic acid extension reactions to take place without washing. When a nucleotide with a fluorescent dye attached to the phosphoric acid site is used, the phosphoric acid site is cleaved after extension reaction, so that fluorescence measurement can be performed serially without quenching. The resulting fluorescence is observed serially, so that a so-called real-time reaction scheme can be realized. Also, JP Patent Application No. 2009-266920 (applied by the applicant) more specifically describes protocols for a real-time single molecular sequencing reaction. Since a base extension reaction proceeds simultaneously with the introduction of necessary reagents in these methods, solution delivery should be controlled for each visual field or the next substrate should be set after a first measurement is completed.
In such a case, an example of a method for topically controlling the initiation of base extension reaction is a method that involves disposing a protecting group cleavable by light irradiation at position 3′ of a probe, as described in Patent document 2. According to Patent document 2, a caged compound is disposed as a protecting group at position 3′ on the oligo probe side, the protecting group is cleaved by UV irradiation, and thus a real-time base extension reaction is initiated. With the use of this method, a base extension reaction is inhibited at the stage at which no UV irradiation is performed, and the reaction can be initiated by UV irradiation.
In the case of a reaction system in which base extension is initiated by light irradiation, only a visual observation field (visual measurement field) should be irradiated with light while light is prevented from leaking to the other parts. The nucleic acid analysis device according to the embodiment is effective for such purpose.
In an immobilization step 711 for immobilizing a template DNA onto a nucleic acid analysis device, a template DNA, primers, and an enzyme are immobilized onto the nucleic acid analysis device. Biotin-avidin binding, thiol-gold chemical binding, or the like can be used for such an immobilization method. Also, as previously described in Background Art, a technique that involves regularly disposing beads, metal structures, or the like in advance on a substrate, and immobilizing a template DNA thereon has already been commercialized.
In set step 712 for setting the nucleic acid analysis device to an apparatus, the nucleic acid analysis device treated as described above is set to an apparatus with which fluorescence can be observed using evanescent light as excitation light as described in Embodiment 1. At this time the connection of a solution delivering system, focus adjustment for the observation optical system, and the like are completed in advance.
A supply step 713 for supplying a reaction agent is a step for delivering a reaction reagent to flow channels of a nucleic acid analysis device. During the step, fluorescence labeled dNTP is applied to initiate a base extension reaction. The dNTP to be used herein has a structure wherein a phospholink nucleotide is linked to the terminal phosphoric acid, so that an enzyme cleaves the fluorescent dye in the process of base incorporation. When the protecting group attached for the inhibition of the initiation of the reaction is cleaved by light irradiation, base extension reactions are serially performed and thus every time when a base is incorporated, fluorescence labeling the nucleotide is detected.
Next, a shift step 714 for shifting (from a current observation field) to the next visual observation field (visual measurement field) is a procedure for sequentially shifting visual observation fields on a nucleic acid analysis device having a plurality of visual observation fields. Examples of such a method for shifting visual fields include a method that involves shifting a nucleic acid analysis device using an XY stage and a method that involves moving an observation optical system. In association with the shifting of visual fields, readjustment of the focus of an optical system may be required.
Subsequently, an observation step 715 for observing reaction initiation and base extension reaction is performed. When light is irradiated for cleaving a protecting group, a real-time base extension reaction is initiated. The fluorescence signals of the real-time base extension reaction are consecutively observed and then the base sequence information is collected. A visual field should be fixed until the completion of single real-time base-extension sequencing. The time required for single sequencing is thought to range from about 0 to 60 minutes based on the time taken for the loss of the enzyme activity.
When the enzyme activity is lost to make the observation of the extension reaction difficult, a determination step 716 for determining if the observation of all visual fields is completed (?) is performed. Until the completion of the observation of all visual fields, the shift step 714 (step for shifting to the next visual observation field) to the determination step 716 for determining if the observation of all visual fields is completed (?) are repeated, so that real-time base extension reaction and observation are repeated.
After completion of the observation of all visual fields, a washing step 717 for washing the nucleic acid analysis device is performed, and then reagents and the like remaining within the nucleic acid analysis device are discharged. After completion of the treatment, a removal step 718 for removing the nucleic acid analysis device is performed.
As shown in
In addition, when a laser is used as irradiation light, a laser beam-irradiation field can be rectangular-shaped or square-shaped by a technique of laser homogenization. In this case, the intervals of reaction spot groups 901 can be narrowed as long as the irradiation field does not affect the neighboring visual fields.
Regarding the real-time base extension reaction, Embodiment 4 describes an example of controlling reaction initiation through disposition of a protecting group cleavable by light irradiation. Meanwhile, in a reaction system not using any protecting group, a reaction is initiated simultaneously with the introduction of a solution containing primers labeled with fluorescent molecules. In the case of such a reaction system, a real-time base extension reaction proceeds even on reaction spots that have not yet been observed. Hence, the reaction system cannot be used in flow channels described in Embodiment 4. Reaction initiation should be controlled by devising a solution delivery method in the case of the reaction system wherein the reaction proceeds simultaneously with solution introduction. The nucleic acid analysis device according to the embodiment is also effective for such a case.
All publications, patents, and patent applications cited herein are incorporated herein by reference in their entirety.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2009-127907 | May 2009 | JP | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/JP2010/058710 | 5/24/2010 | WO | 00 | 11/23/2011 |
