Claims
- 1. An isolated nucleic acid encoding a polypeptide for bovine epidermal growth factor.
- 2. The isolated nucleic acid according to claim 1, wherein the encoded polypeptide comprises an amino acid sequence selected from the group consisting of
a) an amino acid sequence depicted in SEQ ID NO: 9; b) an amino acid sequence depicted in SEQ ID NO: 11; and c) a functionally equivalent amino-acid sequence having at least 55% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 3. The isolated nucleic acid as set forth in claim 2, wherein the encoded polypeptide comprises a functionally equivalent amino acid sequence having at least 60% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 4. The isolated nucleic acid as set forth in claim 3, wherein the encoded polypeptide comprises a functionally equivalent amino acid sequence having at least 65% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 5. The isolated nucleic acid as set forth in claim 4, wherein the encoded polypeptide comprises a functionally equivalent amino acid sequence having at least 70% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 6. The isolated nucleic acid as set forth in claim 5, wherein the encoded polypeptide comprises a functionally equivalent amino acid sequence having at least 75% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 7. The isolated nucleic acid as set forth in claim 6, wherein the encoded polypeptide comprises a functionally equivalent amino acid sequence having at least 80% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 8. The isolated nucleic acid as set forth in claim 7, wherein the encoded polypeptide comprises a functionally equivalent amino acid sequence having at least 85% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 9. The isolated nucleic acid as set forth in claim 8, wherein the encoded polypeptide comprises a functionally equivalent amino acid sequence having at least 90% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 31.
- 10. The isolated nucleic acid as set forth in claim 9, wherein the encoded polypeptide comprises a functionally equivalent amino acid sequence having at least 95% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 11. The isolated nucleic acid as set forth in claim 10, wherein the encoded polypeptide comprises a functionally equivalent amino acid sequence having at least 99% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 12. The isolated nucleic acid as set forth in claim 1, wherein the nucleic acid comprises the nucleotide sequence selected from the group consisting of
a) a nucleotide sequence depicted in SEQ ID NO: 8; b) a nucleotide sequence depicted in SEQ ID NO: 10; and c) a functionally equivalent nucleotide sequence having at least 75% homology to the nucleotide sequence depicted in SEQ ID NO: 8 or to the nucleotide sequence depicted in SEQ ID NO: 10.
- 13. The isolated nucleic acid as set forth in claim 12, wherein the nucleic acid comprises a nucleotide sequence having at least 80% homology to the nucleotide sequence depicted in SEQ ID NO: 8 or SEQ ID NO: 10.
- 14. The isolated nucleic acid as set forth in claim 13, wherein the nucleic acid comprises a nucleotide sequence having at least 85% homology to the nucleotide sequence depicted in SEQ ID NO: 8 or SEQ ID NO: 10.
- 15. The isolated nucleic acid as set forth in claim 14, wherein the nucleic acid comprises a nucleotide sequence having at least 90% homology to the nucleotide sequence depicted in SEQ ID NO: 8 or SEQ ID NO: 10.
- 16. The isolated nucleic acid as set forth in claim 15, wherein the nucleic acid comprises a nucleotide sequence having at least 95% homology to the nucleotide sequence depicted in SEQ ID NO: 8 or SEQ ID NO: 10.
- 17. The isolated nucleic acid as set forth in claim 16, wherein the nucleic acid comprises a nucleotide sequence having at least 99% homology to the nucleotide sequence depicted in SEQ ID NO: 8 or SEQ ID NO: 10.
- 18. A fragment of an isolated nucleic acid encoding a polypeptide for bovine epidermal growth factor, wherein the fragment comprises a functionally equivalent nucleotide sequence as the nucleotide sequence depicted in SEQ ID NO: 10.
- 19. A fragment of the isolated polypeptide for bovine epidermal growth factor, wherein the fragment comprises a functionally equivalent polypeptide as the amino acid sequence depicted in SEQ ID NO: 11.
- 20. An expression construct capable of directing the expression of a bovine epidermal growth factor in a suitable host cell, wherein the expression construct comprises a nucleic acid as set forth in claim 1 operably linked to control sequences compatible with the host cell.
- 21. A vector comprising a nucleic acid as set forth in claim 1.
- 22. A host cell comprising a nucleic acid as set forth in claim 1, so that the cell can express a polypeptide for bovine epidermal growth factor encoded by the nucleic acid.
- 23. The host cell according to claim 22, wherein the cell is selected from Aspergillus niger, Aspergillus ficuum, Aspergillus awamori, Aspergillus oryzae, Trichoderma reesei, Mucor miehei, Kluyverromyces lactic, Pichia pastoris, Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis and Bacillus licheniformis, or a plant cell.
- 24. The host cell according to claim 23, wherein the cell is Escherichia coli.
- 25. The host cell as set forth in claim 23, wherein the cell is Pichia pastoris.
- 26. A feed additive comprising a preparation selected from the group consisting of
a) a polypeptide for bovine epidermal growth factor encoded by a nucleic acid; b) a polypeptide for bovine epidermal growth factor encoded by a nucleic acid, wherein the polypeptide for bovine epidermal growth factor is in combination with inert or active ingredients; c) a microorganism or a plant tissue, wherein the microorganism or the plant tissue expresses a polypeptide for bovine epidermal growth factor encoded by a nucleic acid; and d) a culture medium or an extract obtained from the microorganism or the plant tissue, wherein the culture medium or the extract contains a polypeptide for bovine epidermal growth factor encoded by a nucleic acid.
- 27. The feed additive as set forth in claim 26, wherein the encoded polypeptide comprises an amino acid sequence selected from the group consisting of
a) an amino acid sequence depicted in SEQ ID NO: 9; b) an amino acid sequence depicted in SEQ ID NO: 11; and c) a functionally equivalent amino acid sequence having a homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 28. The feed additive as set forth in claim 27, wherein the homology is selected from the group comprising at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, and at least 99%.
- 29. The feed additive as set forth in claim 28, wherein the microorganism is selected from Aspergillus niger, Aspergillus ficuum, Aspergillus awamori, Aspergillus oryzae, Trichoderma reesei, Mucor miehei, Kluyverromyces lactic, Pichia pastoris, Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis and Bacillus licheniformis.
- 30. The feed additive as set forth in claim 29, wherein the microorganism is Escherichia coli.
- 31. The feed additive as set forth in claim 29, wherein the microorganism is Pichia pastoris.
- 32. A feed composition comprising a feedstuff in combination with a polypeptide of bovine epidermal growth factor, or the feedstuff treated with a polypeptide of bovine epidermal growth factor.
- 33. The feed composition as set forth in claim 32, wherein the encoded polypeptide comprises an amino acid sequence selected from the group consisting of
a) an amino acid sequence depicted in SEQ ID NO: 9; b) an amino acid sequence depicted in SEQ ID NO: 11; and c) a functionally equivalent amino acid sequence having a homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 34. The feed composition as set forth in claim 33, wherein the homology is selected from the group comprising at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, and at least 99%.
- 35. The feed composition as set forth in claim 34, wherein the polypeptide of bovine aid epidermal growth factor is in the form of a powder or a liquid.
- 36. The feed composition as set forth in claim 35, wherein the feedstuff is selected from the group consisting of corn, grain, sorghum, wheat, barley, oats, vegetable protein meals, grass, hay, grass filage, and maize silage.
- 37. A method for producing a polypeptide having bovine epidermal growth factor activity comprising the steps of:
a) culturing a host cell comprising a nucleic acid encoding a polypeptide for bovine epidermal growth factor under conditions conducive to the expression of the polypeptide encoded by the nucleic acid; and b) recovering the encoded polypeptide from the host cell, from the culture medium comprising the host cell, or from an extract obtained from the host cell.
- 38. The method as set forth in claim 37, wherein the encoded polypeptide comprises an amino acid sequence selected from the group consisting of
a) an amino acid sequence depicted in SEQ ID NO: 9; b) an amino acid sequence depicted in SEQ ID NO: 11; and c) a functionally equivalent amino acid sequence having a homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 39. The method as set forth in claim 38, wherein the homology is selected from the group comprising at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, and at least 99%.
- 40. The method as set forth in claim 39, wherein the host cell is selected from Aspergillus niger, Aspergillusficuum, Aspergillus awamori, Aspergillus oryzae, Trichoderma reesei, Mucor miehei, Kluyverromyces lactic, Pichia pastoris, Saccharomyces cerevisiae, Escherichia coli, Bacillus subtilis and Bacillus licheniformis, or a plant cell.
- 41. The method as set forth in claim 40, wherein the host cell is Escherichia coli.
- 42. The method as set forth in claim 41, wherein the host cell is Pichia pastoris.
- 43. A method for producing a plant which expresses a nucleic acid as set forth in claim 1, by introducing the nucleic acid into a plant cell so that the nucleic acid becomes incorporated into the genome of the plant cell.
- 44. The method as set forth in claim 43, wherein the plant is selected from canola, soybean, corn and potato.
- 45. A method for improving the growth of an animal by administering a polypeptide of bovine epidermal growth factor encoded by a nucleic acid for bovine epidermal growth factor.
- 46. The method as set forth in claim 45, wherein the encoded polypeptide comprises an amino acid sequence selected from the group consisting of
a) an amino acid sequence depicted in SEQ ID NO: 9; b) an amino acid sequence depicted in SEQ ID NO: 11; and c) a functionally equivalent amino acid sequence having a homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 47. The method as set forth in claim 46, wherein the homology is selected from the group comprising at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, and at least 99%.
- 48. The method as set forth in claim 47, wherein the animal is selected from a pig, a piglet, a cow, a calf, a goat, a kid, a sheep, and a lamb.
- 49. The method as set forth in claim 48, wherein the animal is a cow or a calf.
- 50. A method for prevention and treatment of intestinal infection by administering a polypeptide of bovine epidermal growth factor encoded by a nucleic acid to an animal.
- 51. The method as set forth in claim 50, wherein the encoded polypeptide comprises an amino acid sequence selected from the group consisting of
a) an amino acid sequence depicted in SEQ ID NO: 9; b) an amino acid sequence depicted in SEQ ID NO: 11; and c) a functionally equivalent amino acid sequence having a homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 52. The method as set forth in claim 51, wherein the encoded polypeptide has a homology selected from the group comprising at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, and at least 99%. c) a functionally equivalent amino acid sequence having at least 55% homology to the amino acid sequence depicted in SEQ ID NO: 9 or to the amino acid sequence depicted in SEQ ID NO: 11.
- 53. The method as set forth in claim 52, wherein the animal is selected from a pig, a piglet, a cow, a calf, a goat, a kid, a sheep, and a lamb.
- 54. The method as set forth in claim 53, wherein the animal is a cow or a calf.
- 55. The method as set forth in claim 53, wherein the infection is selected from scour, an enteropathogenic E. coli infection, and giardiasis.
- 56. The method as set forth in claim 55, wherein the enteropathogenic E. coli infection is enteric colibacillosis.
- 57. The method as set forth in claim 56, wherein the animal is a calf.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority from U.S. Provisional Patent Application No. 60/292,136, filed May 18, 2001. To the extent that it is consistent herewith, the aforementioned application is incorporated herein by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60292136 |
May 2001 |
US |