Nucleic acid molecules comprising a neocentromere

FIELD OF THE INVENTION

The present invention is directed generally to an isolated nucleic acid molecule encompassing a neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof and its use inter alia in developing a range of eukaryotic artificial chromosomes including mammalian (e.g. human) and non-mammalian artificial chromosomes. Such artificial chromosomes are useful in a range of genetic therapies.

BACKGROUND OF THE INVENTION

Bibliographic details of the publications referred to by author in this specification are collected at the end of the description.

The rapidly increasing sophistication of recombinant DNA technology is greatly facilitating research and development in the medical and allied health fields. A particularly important area is in mammalian including human genetics and the molecular mechanisms behind some genetic abnormalities. Progress in research in this area has been hampered by the lack of a cloned nucleic acid molecule encompassing a human centromere. The identification and cloning of a human centromere will promote the development of techniques for introducing genes into eukaryotic cells and in particular mammalian including human cells and will be an important asset to gene therapy and the development of a range of genetic diagnostic tests.

The centromere is an essential structure for sister chromatid cohesion and proper chromosomal segregation during mitotic and meiotic cell divisions. The centromere of the budding yeast

Saccharomyces cerevisiae

has been extensively studied and shown to be contained within a relatively short DNA segment of 125 bp that is organized into an 3-bp (CDEI) and 26-bp (CDEIII) domain, separated by a 78- to 87-bp, highly AT-rich, middle (CDEII) domain (Clarke and Carbon, 1985). The centromere of the fission yeast

Schizosaccharomyces pombe

is considerably larger, ranging from 40 to 100 kb, and consists of a central core DNA element of 4 to 7 kb flanked on both sides by inverted repeat units (Steiner et al., 1993). Recently, the functional DNA components of a higher eukaryotic centromere have been characterized in a minichromosome from

Drosophila melanogaster

and shown to consist of a 220-kb essential core DNA flanked by 200 kb of highly repeated sequences on one side (Murphy and Karpen, 1995).

The mammalian centromere, like the centromeres of all higher eukaryotes studied to date, contains a great abundance of highly repetitive, heterochromatic DNA. For example, a typical human centromere contains 2 to 4 Mb of the 171-bp α-satellite repeat (Wevrick and Willard 1989, 1991; Trowell et al., 1993), plus a smaller and more variable quantity of a 5-bp satellite III DNA (Grady et al., 1992; Trowell et al., 1993). The role of these satellite sequences is presently unclear. Transfection of a cloned 17-kb uninterrupted α-satellite array into cultured simian cells (Haaf et al., 1992) or a 120-kb α-satellite-containing YAC into human and hamster cells (Larin et al., 1994) appear to confer centromere function at the sites of integration. Other workers have analyzed rearranged Y chromosomes (Tyler-Smith et al., 1993), or dissected the centromere of the human Y chromosome with cloned telomeric DNA (Brown et al., 1994) and suggested that 150 to 200 kb of α-satellite DNA plus ˜300 kb of adjacent sequences are associated with human centromere function. In addition, a human X-derived minichromosome that retained 2.5 Mb of α-satellite array has been produced by telomere-associated chromosome fragmentation (Farr et al., 1995). In all these studies, it is not known whether non-α-satellite DNA sequences are embedded within the centromeric site and operate independently of, or in concert with, the α-satellite DNA.

In mammals, four constitutive centromere-binding proteins, CENP-A, CENP-B, CENP-C, and CENP-D, have been characterized to varying extents and implicated to have possible direct roles in centromere function. CENP-A a protein localized to the outer kinetochore domain, is a centromere-specific core histone that shows sequence homology to the histone H3 protein and may serve to differentiate the centromere from the rest of the chromosome at the most fundamental level of chromatin structure—the nucleosome (Sullivan et a., 1994). CENP-B, a protein which associates with the centromeric heterochromatin through its binding to the CENP-B box motif found in primate α-satellite and mouse minor satellite DNA, probably has a role in packaging centromeric heterochromatic DNA—a role which, however, may not be indispensable since the protein is undetectable on the Y chromosome (Pluta et al., 1990) and is found on the inactive centromeres of dicentric chromosomes (Earnshaw et al., 1989). CENP-C has been shown to be located at the inner kinetochore plate and is postulated to have an essential although yet undetermined centromere function, as seen, for example, from inhibition of mitotic progression following microinjection of anti-CENP-C antibodies into cells (Bernat et al., 1990; Tomkiel et al., 1994) and from its association with the active but not the inactive centromeres of dicentric chromosomes (Earnshaw et al., 1989; Page et al., 1995; Sullivan and Schwartz 1995). Finally, CENP-D (or RCC1) is a guanine exchange factor that appears to have a general cellular role that is neither specific nor clear for the centromere (Kingwell and Rattner 1987; Bischoff et al., 1990; Dasso, 1993). More recently, a new role for the mammalian centromere as a “marshalling station” for a host of “passenger proteins” (such as INCENPs, MCAK, CENP-E, CENP-F, 3F3/2 antigens, and cytoplasmic dynein), has been recognized (reviewed by Earnshaw and Mackay, 1994, and Pluta et al., 1995). These passenger proteins, whose appearance at the centromere is transient and tightly regulated by the cell cycle, provide vital functions that include motor movement of chromosomes, modulation of spindel dynamics, nuclear organization, intercellular bridge structure and function, sister chromatid cohesion and release, and cytokinesis. At present, except for CENP-B, none of the constitutive or passenger proteins have been demonstrated to bind mammalian centromere DNA directly.

In work leading up to the present invention, the inventors identified in a patient (hereinafter referred to as “BE”) an unusual human marker chromosome, mardell 10, which is 100% stable in mitotic division both in patient BE and in established fibroblast and transformed lymphoblast cultures. In accordance with the present invention, a region of the mardel (10) chromosome has been cloned together with the corresponding region from a normal human subject. The nucleic acid molecules cloned contain no substantial α-satellite repeats yet are mitotically stable. The nucleic acid molecules encompass therefore, a new form of centromere referred to herein as a “neocentromere”. The identification and cloning of a eukaryotic neocentromere without substantial α-satellite DNA repeat sequences now provides the means of generating a range of eukaryotic artificial chromosomes such as mammalian including human artificial chromosomes with uses in genetic therapy, transgenic plant and animal production and recombinant protein production. A range of diagnostic reagents is now also obtainable using the cloned neocentromere.

SUMMARY OF THE INVENTION

Sequence Identity Numbers (SEQ ID NOs.) for the nucleotide sequences referred to in the specification are defined following the bibliography.

Throughout this specification and the claims which follow, unless the context requires otherwise, the word “comprise”, or variations such as “comprises” or “comprising”, will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers.

A fibroblast cell line 920158 carrying the mardel marker chromosome was deposited at the European Collection of Cell Cultures (ECACC), Centre for Applied Microbiology Research, Salisbury, Wiltshire, SP4 0JG, UK on May 1, 1997 under Accession No. 97051716. Bacterial artificial chromosomes (BACs) carrying portions of the mardel (10) chromosome have also been deposited at ECACC as follows:

BAC/E8-1: deposited on May 5, 1998 under Accession Number 980505016;

BAC/F2-14: deposited on May 5, 1998 under Accession Number 980505017.

A number of human fibrosarcoma cell lines carrying various neocentromeric constructs were deposited at ECACC as described hereafter by Accession Number with the date of deposit in parenthesises.

HT-38

98050704 (7 May 1998)

HT-47

98050705 (7 May 1998)

HT-54

98050706 (7 May 1998)

HT-190

98050707 (7 May 1998)

HT-191

98050708 (7 May 1998).

One aspect of the present invention provides an isolated nucleic acid molecule comprising a sequence of nucleotides derived from a eukaryotic chromosome and encompassing a neocentromere or a functional derivative synthetic or hybrid form thereof which nucleic acid molecule or its derivatives, synthetic forms or hybrid forms when introduced into a compatible cell is capable of replicating, acting as an extra-chromosomal element and segregating the cell division.

Another aspect of the present invention contemplates a nucleic acid molecule or its chemical equivalent having a tertiary structure which defines a human neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or its mammalian or non-mammalian homologue.

Yet a further aspect of the present invention is directed to an isolated nucleic acid molecule comprising a sequence of nucleotides encompassing a neocentromere derived from a eukaryotic chromosome, which nucleic acid molecule when introduced into a compatible cell is a replicating, extra-chromosomal element which segregates with cell division.

Still another aspect of the present invention is directed to an isolated nucleic acid molecule having a sequence of nucleotides or their chemical equivalents which directs a conformation defining a human neocentromere or a functional derivative thereof or a latent; synthetic or hybrid form thereof or a mammalian or non-mammalian homologue thereof wherein the neocentromere associates with centromere binding proteins (CENP) -A and CENP-C or antibodies thereto and does not contain substantial α-satellite DNA repeat sequences.

A further aspect of the present invention is directed to an isolated nucleic acid molecule comprising a nucleotide sequence encompassing a neocentromere or a functional derivative, synthetic or hybrid form thereof which when said nucleic acid molecule is in linear form and co-introduced into a cell together with a telomeric sequence, is capable of replicating, remaining as an extra-chromosomal element and segregates with cell division.

Another aspect of the present invention provides an isolated nucleic acid molecule or a derivative, synthetic or hybrid form thereof comprising a sequence of nucleotides:

(i) which directs conformation defining a human neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or its mammalian or non-mammalian homologue wherein said neocentromere is capable of associating with CENP-A and CENP-C;

(ii) which contains no substantial α-satellite DNA sequence repeat; and

(iii) which is capable, when introduced into compatible cells, of replication, remaining extra-chromosomal and segregating with cell division.

Even yet another aspect of the present invention is directed to a genetic construct comprising an origin of replication for a eukaryotic cell and a nucleic acid molecule encompassing a human neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or its mammalian or non-mammalian homologue flanked by telomeric nucleotide sequences functional in the cell in which the genetic construct is to replicate and wherein said genetic construct when introduced into a cell is a replicating, extra-chromosomal element which segregates with cell division.

Another aspect of the present invention is directed to a genetic construct in the form of a eukaryotic artificial chromosome such as a mammalian artificial chromosome (MAC), a human artificial chromosome (HAC) or comprising an origin of replication and a sequence of nucleotides which:

(i) directs a conformation defining a human neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof wherein said neocentromere is capable of associating with CENP-A and CENP-C or antibodies thereto; and

(ii) contains no substantial α-satellite DNA repeat sequences;

said sequence of nucleotides flanked by eukaryotic (e.g. mammalian) telomeric sequences which are in turn flanked by yeast telomeric sequences wherein a unique enzyme site is located between the human and yeast telomeric nucleotide sequences such that upon contact with said enzyme, the yeast telomeric sequences are removed and the eukaryotic (e.g. mammalian) telomeric sequences are exposed.

Still another aspect of the present invention provides a genetic construct comprising an origin of replication and a first nucleic acid molecule defining a human neocentromere or a functional derivative thereof or latent, synthetic or hybrid form thereof, a second nucleic acid molecule encoding a peptide, polypeptide or protein, wherein said first and second nucleic acid molecules are flanked by a first set of eukaryotic (e.g. mammalian, such as human) telomeric sequences which are in turn flanked by a second set of eukaryotic (e.g. yeast) telomeric sequences wherein there are unique enzyme sites between the first and second telomeric sequences such that upon contact with a required enzyme, the second telomeric sequences are cleaved off to expose the first telomeric sequences.

BRIEF DESCRIPTION OF THE FIGURES

FIGS. 1A-1C

are schematic representations showing identification of a YAC contig spanning the marker centromere region. (

FIG. 1A

) Comparison of GTL banding patterns of mardel 10 and normal chromosome 10. The pair of open arrows indicate the two breakpoints on a normal chromosome 10 in generating the marker chromosome (Voullaire et al., 1993). The long and short arms of the marker chromosome are designated q′ and p′, respectively, to distinguish them from the q and p arms of the normal chromosome 10. Asterisk denotes the position of a cosmid 10pC38 that was used to “tag” the q′-arm of stretched marker chromosomes in the ANTI-CEN/FISH experiments. (

FIG. 1B

) A4-megabase YAC contig (#082) from 10q25.2 region that spans the marker centromere. The tilling path of YACs #0 to #23 and their corresponding CEPH library addresses are shown. (

FIG. 1C

) FISH mapping of selected YAC clones from contig #082 using normal fluorescence microscopy and standard metaphase chromosomes prepared from transformed lymphoblast cells of patient BE. The distribution of FISH signals (vertical axis) is shown as a percentage of the signals on one arm of the marker chromosome that is in excess of those found on the opposite arm of the chromosome. The total number of fluorescence signals scored for each of the YAC clones is indicated in brackets.

FIGS.

2

A(

1

)-

2

C(

e

) are photographic representations showing ANTI-CEN/FISH analysis of the marker centromere. (FIGS.

2

A(

1

)-

2

A(

2

)) Detection of α-satellite DNA using a mixture of α-satellite DNA probes (red signals) under low stringency conditions. Centromeres were counter-labelled with CREST#6 autoimmune antibody (pale blue dots; or white when superimposed on a red background). Chromosomes were prepared from transformed lymphoblast cells of patient BE. The right-hand panel represents green pseudo-coloring of DAPI images of chromosomes to provide a better definition of chromosome outline. Only the signal for the antibody, but not that for α-satellite, was seen on the marker centromere (arrowed). (FIGS.

2

B(

1

)-

2

B(

2

)) Simultaneous labelling of stretched human metaphase chromosomes with CREST#6(red) and anti-CENP-C antibody, Am-C1 (pale blue), with the white color indicating full coincidence of the two antibody signals. (FIGS.

2

C(

a

)-

2

C(

e

)) Detection of CENP-C on the marker chromosome. Simultaneous labelling of the marker chromosome (arrowhead) with (FIG.

2

C(

a

)) Am-C1 (pale blue) and (FIG.

2

C(

b

)) CREST#6 (red). (FIG.

2

C(

c

)) Combined images of a and b, showing complete coincidence of Am-C1 and CREST#6 signals. (FIG.

2

C(

d

)) FISH analysis of the same cell as a-c using the 10pC38 cosmid probe (pale blue dots and green arrows) to identify the marker chromosome. Some loss of ANTI-CEN signal, especially for the Am-C1 antibody was seen following FISH. (FIG.

2

C(

e

)) Green pseudo-coloring of DAPI images. A colour photograph corresponding to this figure is available upon request.

FIGS.

3

A(

a

1

)-

3

B(

2

) are photographic representations showing ANTI-CEN/FISH analysis of cosmid clones on stretched (FIGS.

3

A(

a

1

)-

3

A(

f

2

)) and superstretched (FIGS.

3

B(

1

)-

3

B(

2

)) metaphase chromosomes. (FIGS.

3

A(

a

1

)-

3

A(

c

2

)) Examples of cosmid signals (white arrows) localized to the q-region of the marker centromere. (FIGS.

3

A(

d

1

)-

3

A(

f

2

)) Examples of cosmid signals (white arrows) localized to the p′-region of the marker centromere. Green arrows indicate positions of the 10pC38 cosmid DNA tag used to mark the q′-end of the marker chromosome. (FIGS.

3

B(

1

)-

3

B(

2

)) Mapping of Y6C21 onto a superstretched metaphase chromosome. Not included is the 10pC38 q′-tag signal located further to the left of the chromosomal segment shown. ANTI-CEN signals are in red, FISH signals are in pale blue, and overlapping ANTI-CEN and FISH signals are in white. Each of the pictures is accompanied by DAPI images of chromosomes pseudo-coloured in green. A colour photograph corresponding to this figure is available upon request.

FIGS. 4A-4C

Localization of the anti-centromere antibody-binding domain.

FIG. 4A

, Relative positions of different cosmid and PAC clones within the YAC #082 contig, using YAC-3 as a reference. Cosmids are designated as YnCm, where ‘n’ denotes the YAC of origin and ‘m’ denotes the cosmid number. PACs 1-5 are five different PAC clones isolated from a human PAC library (Genome Systems Inc). “HC-contig” represents a group of overlapping cosmids that map tightly around the marker centromere in ANTI-CEN/FISH experiments. A genomic map corresponding to the depicted YAC region was derived from the DNA of patient BE and shown above the YAC map. S, SAlI; K, KspI; N, NotI; Sf, SfiI.

FIG. 4B

, Cumulative scoring of FISH signals in ANTI-CEN/FISH experiments for cosmids Y3C64, Y6C8, Y3C94, Y7C14, Y4C45, Y6C10, Y6C21, Y3C3, PAC5, Y13C1, Y13C8, and Y17C6. The distribution of FISH signals (vertical axis) is those found on the opposite arm of the chromosome. The total number of fluorescence signals scored for each of the cosmid clones is indicated in brackets.

FIG. 4

c

, Restriction mapping of the 80-kb region covered by the eight overlapping cosmids of the HC-contig. These eight cosmids were derived from four different YACs (YAC-3, YAC-4, YAC-6, and YAC-7) and provided independent confirmation of the map. Furthermore, the map agreed fully with the restriction map of a 120 kb-insert PAC clone (PAC4) that spanned the entire HC-contig region. E, EcoRI; R, EcoRV; N, NotI.

FIGS. 5A-5C

are representations showing restriction analysis of genomic DNA of patient BE and those of his normal parents using Y6C10 as probe. DNA was resolved on a PFGE (

FIG. 5A

) or standard agarose gel (FIGS.

5

B and

5

C). Samples 1, 2 and 3 were fibroblast cultures of mother of BE, father of BE, and patient BE, respectively. Sample 4 was a somatic hybrid cell line BE2C1-18-5F containing the marker chromosome. Fragment sizes are in kilobases.

FIGS.

6

A(

1

)-

6

A(

125

), when joined at matchlines A—A through TTT—TTT, represent the full nucleotide sequence (SEQ ID NO:3) of the HC-contig DNA derived from normal human chromosome 10q 25.2 region.

FIGS. 7A-7B

, when joined at matchlines A—A, depict the method used to retrofit YAC3 and YAC5.

FIGS. 8A

to J are diagrammatic representations of the different vectors used for cloning DNA as YACs by the conventional restriction/ligation methods.

FIGS. 9

is a diagrammatic representation of circular TAR summarising the recombination process.

FIG. 10

is a diagrammatic representation showing modification of TAR vector.

FIGS. 11A-11D

, when joined at matchlines A—A through D—D, depict the cloning of 10q25 human neocentromere DNA from mardel (10) chromosome. This DNA is designated NC-contig DNA to distinguish it from the HC-contig derived from the corresponding region of the normal chromosome 10. (

FIG. 11A

) Structural map of the NC-contig region and flanking DNA. Arrows indicate the relative positions and directions of primers used in PCR analyses (Table 3). The restriction sites EcoRI, EcoRV, SrfI, and SftI and SftI are indicated by E, R, Sr and Sf, respectively. The position of the TAR “hook” CE-F2 is represented by the solid box. The hatched bar represents HC- or NC-contig. p′ and q′ refer to the short and long arms of mardel (10), respectively. (

FIG. 11B

) Circular TAR strategy using the vectors pVC39-Alu/C3-F2(+) and pVC39-Alu/C3-F2(−) for the direct cloning of the neocentromere DNA from mardel (10). The position of the Alu consensus sequence hook is represented by the white box. Crosses denote the sites of recombination between the TAR vector and the genomic DNA at the Alu and C3-F2 hooks during cloning. (

FIG. 11C

) Structural maps of the resulting circular YACs 5f-52-E8 and 5f-38-F2 containing the neocentromere DNA of the mardel (10) chromosome. The DNA flanking the NC-contig is represented by stippled bars. (

FIG. 11D

) Structural maps of BAC/E8-1 and BAC/F2-14. Nt represents NotI and URA-BAC-neo represents the retrofitting vector BRV1 (Larionov et al., 1997).

FIG. 12

is a diagrammatic representation specific TAR of HC-region from mardel

10

.

The method was as follows: (1) Co-transformation into YPH857; (2) Select HIS

+

colonies; (3) screen for HC-region by PCR; (4) Prepare high-MW DNA; (5) Digest with I-Scel to expose hTELS; (6) Transfect HT 1080 cells; (7) Select for G418

R

; and (8) analyse by PFGE and FISH.

FIG. 13

is a diagrammatic representation showing cloning in yeast as YAC/HAC.

FIG. 14

is a diagrammatic representation outlining TACT procedure.

FIGS. 15A-15B

are diagrammatic representations of TACT constructs.

FIGS.

16

A(

1

)-

16

A(

37

), when joined at matchlines A—A through J′—J′, depict the full nucleotide sequence (SEQ ID NO:4) of the NC-contig DNA derived from mardel (10) and corresponds to the HC-contig DNA region of the normal chromosome 10.

FIGS.

16

B(

1

)-

16

B(

34

), when joined at matchlines A—A through G′—G′, depict the partial nucleotide sequence of the BAC/F2-14 clone that is derived from a region immediately p′ of the NC-contig DNA (see

FIG. 11D

) (SEQ ID NO:5-29).

SUMMARY OF SEQ ID NOs.

SEQ ID NO.

DESCRIPTION

1

DNA primer

2

DNA primer

3

Nucleotide sequence of HC-contig

4

Nucleotide sequence of NC-contig

5

BAC-F2 contig 1

6

BAC-F2 contig 2

7

BAC-F2 contig 3

8

BAC-F2 contig 4

9

BAC-F2 contig 5

10

BAC-F2 contig 6

11

BAC-F2 contig 7

12

BAC-F2 contig 8

13

BAC-F2 contig 9

14

BAC-F2 contig 15

15

BAC-F2 contig 33

16

BAC-F2 contig 39

17

BAC-F2 contig 41

18

BAC-F2 contig 42

19

BAC-F2 contig 44

20

BAC-F2 contig 47

21

BAC-F2 contig 47 fragment 1

22

BAC-F2 contig 47 fragment 2

23

BAC-F2 contig 47 fragment 3

24

BAC-F2 contig 47 fragment 4

25

BAC-F2 contig 47 fragment 5

26

BAC-F2 contig 47 fragment 6

27

BAC-F2 contig 47 fragment 7

28

BAC-F2 contig 47 fragment 8

29

BAC-F2 contig 47 fragment 9

ABBREVIATIONS USED IN THE SUBJECT SPECIFICATION

mardel (10):

Marker chromosome from patient BE;

comprises a rearrangement of chromosome 10.

HAC:

Human artificial chromosome

YAC:

Yeast artificial chromosome

MAC:

Bacterial artificial chromosome

PLAC:

Plant artificial chromosome

neocentromere:

A centromere containing no substantial

α-satellite DNA

CENP:

Centromere binding protein

HC-contig:

Region of normal chromosome 10 comprising

neocentromere

E8:

q′ end/region of mardel (10) neocentromere

F2:

p′ end/region of mardel (10) neocentromere

BE:

Patient from which mardel (10) identified

TAR:

Transformation-associated recombinant

PCR:

Polymerase chain reaction

Marker neocentromere:

neocentromere on mardel (10).

NC-contig

region of mardel (10) chromosome comprising

neocentromere

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

The present invention is predicated in part on the identification and isolation of nucleic acid molecules exhibiting neocentromeric properties. In accordance with the present invention, a neocentromere is considered a centromere which does not contain substantial α-satellite DNA repeat sequences and, when activated, is capable of functioning as a centromere. The term “substantial” in this context means that the nucleic acid molecule does not contain detectable α-satellite by FISH analysis under medium stringency conditions. The neocentromere may contain a small number of highly diversed α-satellite DNA. In primates, α-satellite DNA is consider 171 bph in length. An nucleic acid molecule containing an activated neocentromere or a neocentromere otherwise functioning as a centromere facilitates in accordance with the present invention, the nucleic acid molecule replicating, remaining extra-chromosomal and segregating with cell division. Reference herein to “neocentromere” is taken to mean a centromere substantially devoid of α-satellite DNA repeat sequences.

Accordingly, one aspect of the present invention provides an isolated nucleic acid molecule comprising a sequence of nucleotides which defines an eukaryotic neocentromere.

More particularly the present invention provides an isolated nucleic acid molecule comprising a sequence of nucleotides derived from a eukaryotic chromosome and encompassing a neocentromere which nucleic acid molecule when introduced into a compatible cell is capable of replicating, acting as an extra-chromosomal element and segregating with cell division.

The present invention is exemplified herein by the identification and cloning of a human neocentromere. This is done, however, with the understanding that the present invention extends to all eukaryotic neocentromeres such as from mammalian, plant, aviary, insect, fungal, yeast and reptilian chromosomes. The most preferred neocentromere, however, is from human chromosomes and their mammalian homologues.

The present invention is predicated in part on the identification of an unusual chromosomal marker in a patient designated “BE”. The chromosomal marker is referred to as “mardel (10)” and results from a rearrangement of human chromosome 10. The mardel (10) marker is mitotically stable and, in accordance with the present invention, contains a functional neocentromere at a location regarded as non-centromeric. The neocentromere at mardel (10) is located between q24 and q26 on chromosome 10 and more particularly around q25. Even more particularly, the neocentromere maps to q25.2 on chromosome 10. The present invention is exemplified by DNA cloned from the q24-q26 region of the mardel (10) chromosome as well as the corresponding region on normal human chromosome 10. These DNA molecules contain a functional neocentromere. The present invention extends, however, to any neocentromere or any chromosome in mammalian and non-mammalian animals as well as plants, yeasts and fungi.

For convenience, the DNA clones from the mardel (10) chromosomes as well as from normal human chromosome 10 are summarised in FIG.

11

. The neocentromere located at or around 10q25 is located on a clone designated the “HC-contig”. DNA clones from mardel (10) are referred to as “E8” or the “NC-contig” which extends from the long arm (q′) of mardel (10) towards the short arm (p′). Clone F2 extends further p′ from E8 (see FIG.

11

). It is emphasised, however, that the present invention extends to any neocentromere on any human chromosome as well as neocentromeres on other mammalian and non-mammalian chromosomes including chromosomes from plants, insects, reptiles, yeast and fungi.

The present invention further contemplates a nucleic acid molecule or its chemical equivalent having a tertiary structure which defines a human neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or its mammalian or non-mammalian homologue.

Even more particularly, the present invention is directed to an isolated nucleic acid molecule having a sequence of nucleotides or their chemical equivalents which directs a conformation defining a human neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or its mammalian or non-mammalian homologue wherein the centromere associates with centromere binding proteins (CENP)-A and CENP-C or antibodies thereto.

Reference herein to “latent” in relation to a centromere includes reference to a centromere not normally functional but nevertheless activatable under certain conditions. A latent centromere may also be considered as a neocentromere provided it has no substantial α-satellite DNA repeat sequences.

The size of the neocentromere in accordance with the present invention may range from about 50 bp to about 1500 kbp, from about 70 bp to about 1000 kbp, from about 75 bp to about 800 kpb, from about 80 bp to about 500 kbp, from about 85 bp to about 200 kbp, from about 90 bp to about 100 kbp, from about 100 bp to about 1 kbp, about 120 bp to about 500 bp, about 180 bp to about 300 bp. In one particular embodiment, the centromere is approximately 60-100 kbp. In another embodiment, the centromere is about 80 kbp.

The nucleic acid molecule encompassing the HC-contig for human chromosome

10

of the present invention set forth in

FIG. 6

(SEQ. ID NO: 3). The nucleic acid molecule encompassing the NC-contig (part of E8) form mardel (10) is set forth in

FIG. 16A

(SEQ ID NO: 4). The nucleic acid molecule encompassing F2 of mardel (10) is set forth in

FIG. 16B

as separate contigs (SEQ ID NOs: 5-29). The nucleic acid molecules have a tertiary structure and the neocentromere is a conformation of nucleotides within this tertiary structure. Accordingly, the neocentromere is not defined by a linear sequence of nucleotides although this aspect of the present invention is exemplified using the nucleotide sequence set forth in

FIGS. 6

,

16

A and

16

B, the subject invention extends to any sequence directing a conformation defining a centromere and hybridising to the sequence set forth in one or more of

FIGS. 6

,

16

A and/or

16

B under low stringency conditions at 42° C. and/or which comprises a nucleotide sequence having at least about 40% nucleotide similarity to one or more sequences set forth in

FIGS. 6

,

16

A and/or

16

B. Preferably, the percentage similarity is at lest about 50%, more preferably at least about 60%, still more preferably at least about 70%, even more preferably at least about 80-90% or above such as 95%, 97%, 98% and 99%.

Another embodiment of the present invention is directed to YAC3 and YAC5 encompassing the HC contig and flanking sequence as well as nucleotide sequences related to YAC3 and/or YAC5 at the homology, similarity or hybridization levels.

Reference herein to a low stringency at 42° C. includes and encompasses from at least about 1% v/v to at least about 15% v/v formamide and from at least about 1M to at least about 2M salt for hybridisation, and at least about 1M to at least about 2M salt for washing conditions. Alternative stringency conditions may be applied where necessary, such as medium stringency, which includes and encompasses from at least about 16% v/v to at least about 30% v/v formamide and from at least about 0.5M to at least about 0.9M salt for hybridisation, and at least about 0.5M to at least about 0.9M salt for washing conditions, or high stringency, which includes and encompasses from at least about 31% v/v to at least about 50% v/v formamide and from at least about 0.01M to at least about 0.15M salt for hybridisation, and at least about 0.01M to at least about 0.15M salt for washing conditions. These stringency conditions may be altered dependent on the source of DNA and other factors.

The term “similarity” as used herein includes exact identity between compared sequences at the nucleotide level. Where there is non-identity at the nucleotide level, “similarity” includes differences between sequences which nevertheless result in conformation defining a functional neocentromere.

The nucleic acid molecule of the present invention may comprise a naturally occurring nucleotide sequence from a healthy human subject or may comprise the nucleotide sequence from a human subject exhibiting one or more chromosomal-dependant conditions such as a subject carrying mardel

10

chromosome or a chromosome conferring an equivalent or similar condition or may carry one or more nucleotide substitutions, deletions and/or additions relative to the naturally or non-naturally occurring sequence. Such modifications are referred to herein as “derivatives” and include mutants, fragments, parts, homologues and analogues of the naturally occurring nucleotide sequence. Preferably, the derivatives of the present invention still define a functional neocentromere.

Reference herein to a “neocentromere” includes reference to a functional neocentromere or a functional derivative thereof meaning that it is capable of facilitating sister chromatid cohesion and chromosomal segregation during mitotic cell divisions and/or is capable of associating with CENP-A and/or CENP-C and/or is capable of interacting with anti-CENP-A antibodies or anti-CENP-C antibodies. Generally, and preferably, the neocentromere is incapable of interacting with CENP-B or anti-CEP-B antibodies. Alternatively, the neocentromere may be a latent centromere capable of activation by epigenetic mechanisms. The neocentromere may also be a hybrid of other human, mammalian, plant or yeast neocentromeres. Synthetic neocentromeres provided by, for example, polymeric techniques to arrive at the correct confromation are also contemplated by the present invention. All such forms and definitions of neocentromere are encompassed by use of this term.

Another aspect of the present invention provides an isolated nucleic acid molecule or chemical equivalent having the following characteristics:

(i) comprises a nucleotide sequence or chemical equivalent directing a conformation which defines a neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or;

(ii) comprises a nucleotide sequence or chemical equivalent substantially as set forth in one or more of

FIGS. 6

,

16

A and/or

16

B or having at least about 40% similarity thereto or capable of hybridising thereto under low stringency conditions at 42° C.; and

(iii) comprises a neocentromere capable of associating with CENP-A or CENP-C or antibodies thereto.

Preferably, the neocentromere is incapable of interacting with CENP-B or antibodies thereto.

In a particularly preferred embodiment, the centromere corresponds to a human genomic region which maps between q24 and q26 on chromosome 10, and in particular q25 on chromosome 10.

The nucleic acid molecule or its chemical equivalent of the present invention defining a conformational neocentromere or functional derivative thereof or latent, synthetic or hybrid form thereof is useful inter alia of the generation of artificial chromosomes such as human artificial chromosomes (HACs), mammalian artificial chromosomes (MACs), yeast artificial chromosomes (YACs) and plant artificial chromosomes (PLACs). HAC's are particularly useful since they are capable of accommodating large amounts of DNA and are capable of propagation in human cells. The HAC's are non-viral in origin and, hence, are more suitable for gene therapy by, for example, introducing therapeutic genes. Furthermore, the HACs remain extra-chromosomal and, hence, have no insertional/substitutional mutagenic potential. The essence of a HAC is the presence of a neocentromere or latent, synthetic or hybrid form thereof which enables stable segregation during cell division. The HAC also remains extra-chromosomal and, hence, is more suitable for gene therapy. Reference to “extra-chromosomal” means that it does not integrate into the main chromosome and, in effect, is episomal.

Accordingly, the present invention provides a genetic construct comprising an origin of replication for a eukaryotic cell and a nucleic acid molecule encompassing a eukaryotic neocentromere or a functional derivative thereof or a latent, synthetic, hybrid form thereof or its mammalian or non-mammalian homologue flanked by telomeric nucleotide sequences functional in the cell in which the genetic construct is to replicate and wherein said genetic construct when introduced into a cell is a replicating, extra-chromosomal element which segregates with cell division.

More particularly, the present invention further contemplates a genetic construct in the form of an artificial chromosome comprising an origin of replication for a mammalian, human, plant or yeast cell and a nucleic acid molecule encompassing a human neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or its mammalian or non-mammalian homologue flanked by telomeric nucleotide sequences functional in the cell in which the artificial chromosome is to replicate.

Another embodiment provides a genetic construct in the form of an artificial chromosome comprising an origin of replication for a mammalian, human, plant or yeast cell and a nucleic acid molecule having a tertiary structure which defines a human neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or its mammalian homologue flanked by telomeric sequences functional in the cell in which the artificial chromosome is to replicate.

Yet another embodiment is directed to a genetic construct in the form of an artificial chromosome comprising an origin of replication for a mammalian, human, plant or yeast cell and a nucleic acid molecule having a sequence of nucleotides which directs a conformation defining a human neocentromere wherein the centromere associates with CENP-A and/or CENP-C or antibodies thereto and does not contain substantial α-satellite DNA repeat sequences, said nucleic acid molecule flanked by telomeric nucleotide sequences functional in the cell which the artificial chromosome is to replicate.

Still yet another aspect of the present invention relates to a genetic construct in the form of an artificial chromosome comprising an origin of replication for a mammalian, human, plant or yeast cell and a nucleic acid molecule comprising a sequence of nucleotides which:

(i) directs a conformation which defines a neocentromere or a functional form thereof or a latent, synthetic or hybrid form thereof;

(ii) comprises a nucleotide sequence substantially as set forth in one or more of

FIGS. 6

,

16

A and/or

16

B or having at least about 40% similarity to the nucleotide sequences set forth in

FIGS. 6

,

16

A and/or

16

B or is capable of hybridising to one or more of these sequences under low stringency conditions at 42° C.;

wherein the neocentromere is capable of associating with CENP-A and/or CENP-C antibodies thereto and wherein said nucleic acid molecule is flanked by telomeric nucleotide sequences functional in the cell in which the artificial chromosome replicates.

In a preferred embodiment, the genetic construct is a HAC and comprises human telomeric sequences. In a particularly preferred embodiment, the HAC further comprises yeast artificial chromosome (YAC) arms and, hence, becomes a HAC/YAC shuttle vector capable of propagation in human and yeast cells. Preferably, the HAC/YAC contains a unique enzyme site between yeast telomeric sequences and human telomeric sequences such that upon contact with the particular enzyme, the yeast telomeric sequences are removed leaving the human telomeric sequences. Preferably, the unique enzyme site is a yeast specific enzyme site such as I-SceI.

According to this embodiment, there is provided a genetic construct defining a HAC/YAC comprising an origin of replication and a nucleic acid molecule encompassing a human neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or a mammalian or non-mammalian homologue thereof, said nucleic acid molecule flanked by human telomeric sequences which are in turn flanked by yeast telomeric sequences wherein a unique enzyme site is located between the human and yeast telomeric nucleotide sequences such that upon contact with the enzyme, the yeast telomeric sequences are removed and the human telomeric sequences are exposed.

More particularly, the present invention is directed to a genetic construct defining a HAC/YAC comprising an origin of replication and a nucleic acid molecule encompassing a human centromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or a mammalian or non-mammalian homologue thereof wherein the neocentromere associates with CENP-A and/or -C or antibodies thereto and does not contain substantial α-satellite DNA sequences wherein said nucleic acid molecule is flanked by human telomeric sequences which are in turn flanked by yeast telomeric sequences wherein a unique enzyme site is located between the human and yeast telomeric nucleotide sequences such that upon contact with said enzyme, the yeast telomeric sequences are removed and the human telomeric sequences are exposed.

Even more particularly, the present invention is directed to a genetic construct in the form of a HAC/YAC comprising an origin of replication and a sequence of nucleotides which directs a conformation defining a human neocentromere or a functional derivative thereof or a latent, synthetic or hybrid form thereof or a mammalian or non-mammalian homologue thereof wherein said neocentromere is capable of associating with CENP-A and/or CENP-C or antibodies thereto, said sequence of nucleotides flanked by human telomeric sequences which are in turn flanked by yeast telomeric sequences wherein a unique enzyme site is located between the human and yeast telomeric nucleotide sequences such that upon contact with said enzyme, the yeast telomeric sequences are removed and the human telomeric sequences are exposed.

Preferably, the length of the nucleotide sequence is between about 30 kpb and 1500 kpb, and more preferably between 60 kbp and 1000 kpb.

In a particularly preferred embodiment, the unique enzyme site is a yeast specific enzyme site such as I-SceI.

The present invention extends to yeast cells and human cells carrying the genetic constructs of the present invention and to proteins produced therefrom.

The genetic constructs may also comprise marker genes and other unique restriction sites to facilitate insertion of adventitious DNA. Accordingly, the genetic constructs of the present invention may further comprise adventitious or heterologous DNA encoding a product of interest. Preferred products of interest include pharmaceutically useful genes such as genes encoding cytokines, receptors, growth regulators and the like. Endogenous genes may also be replaced by wild-type genes or modified genes.

The adventitious or heterologous DNA may also encode a molecule not synthesised in a sufficient amount in a particular subject and hence the increased copy number permits greater amounts of the molecule being synthesised.

Accordingly, the present invention contemplates a genetic construct comprising an origin of replication and a first nucleic acid molecule defining a human neocentromere or a functional derivative thereof or latent, synthetic or hybrid form thereof or a mammalian or non-mammalian homologue, a second nucleic acid molecule encoding a peptide, polypeptide or protein, wherein said first and second nucleic acid molecules are flanked by a first set of human telomeric sequences which are in turn flanked by a second set of yeast telomeric sequences wherein there are unique enzyme sites between the human and yeast telomeric sequences such that upon contact with said enzyme, the yeast telomeric sequences are cleaved off to expose the human telomeric sequences.

Reference herein to segregate preferably means mitotically stable segregation. Conveniently, stable segregation may be determined as the presence of an artificial chromosome in 40-60% of daughter cells after 4-6 months of continuous passage.

The present invention extends to other artificial chromosome analogues to the HACs and HAC/YACs described above such as MACs and PLACs.

Another aspect of the present invention relates to peptides, polypeptides and proteins which bind, interact or otherwise associate with the human neocentromere of the present invention or its mammalian and non-mammalian homologue. Preferably, the molecules are proteins, referred to as primary (1°) proteins. The 1° proteins bind to the neocentromere and secondary (2°) proteins bind to the 1° proteins before or after association with the neocentromere. The identification of the human neocentromere in accordance with the present invention provides a mechanism for assaying 1° proteins and 2° proteins which may be important for screening chromosomes in, for example, genetic disorders. This is particularly the use in Down's Syndrome which results from defective chromosome segregation.

The 1° proteins are readily detected by, for example, a gel shift assay. The nucleic acid molecule of the present invention defining the human neocentromere is digested, labelled and contacted with nuclear extract putatively containing the 1° proteins and resolved on a gel. When a 1° protein binds to a fragment carrying a binding portion of the neocentromere, the DNA fragment migrates in the gel at a slower rate due to the bound protein.

The present invention extends to purified 1° proteins capable of association with the subject centromere and to genetic sequences encoding same and to antibodies thereto.

The neocentromeres of the present invention are readily identified and characterised using, for example, human fibrosarcoma cell lines. For example, DNA suspect of carrying a neocentromere, is introduced into fibrosarcoma cells in a linear form, generally together with a telomeric sequence. The cells are then screened for the presence of replicating, extra-chromosomal and segregating elements, referred to as mini chromosomes.

The present invention further encompasses eukaryotic cells carrying replicating, extrachromosomal and segregation nucleic acid molecules. Preferably the eukaryotic cells are mammalian cells and most preferably human cells. The nucleic acid molecules according to this aspect of the present invention are preferably as herein described. Particularly preferred cells are HT-38, HT-47, HT-54, HT-190, HT-191, BAC/E8-1, and BAC/F2-14.

The present invention is further described by the following non-limiting Figures and Examples.

EXAMPLE 1

YAC and Cosmid Probes for FISH

YACs carrying specific STSs were identified (Moir et al., 1994) by PCR-based screening of YAC libraries prepared in pYAC4 vector at the Center for Genetics in Medicine at Washington University (Brownstein et al., 1989) and at the CEPH (Albertsen et al., 1990). Cosmid DNA inserts (35-40 kb) were ligated to SUPERCOS I vector (Stratagene) and packaged with GIGAPAK III GOLD extract (Stratagene) according to the manufacturer's instructions. YAC probes were prepared by Alu-PCR of total yeast genomic DNA using primers 5′-GGATTACAGG(C/T)(A/G)TGAGCCA-3′ [SEQ ID NO:1] and 5′-(A/G)CCA(C/T)TGCACTGCAGCCTG-3′ [SEQ ID NO:2] according to published method (Archidiacono et al., 1994). For probe labelling, 1 μg of the YAC PCR products or whole cosmid DNA isolated by CsCl centrifugation or Qiagen column was used. The DNA was labelled with Biotin-16-dUTP (Boehringer Mannheim) using a NICK translation kit (Boehinger Mannheim). A probe mix of 6-10μg/ml of biotinylated probe DNA, 300 μg/ml of COT-1 DNA (Boehringer Mannheim), 500 μg/ml of carrier salmon sperm DNA and, where indicated, 10 μg/ml of biotinylated 10pC38 tag DNA was ethanol precipitated, resuspended in a hybridization mix of 50% v/v formamide in 2×SSC and 10% w/v dextran sulphate, denatured at 95° C. for 5 min, preannealed for 30-60 min at 37° C. to suppress repetitive sequences, before adding to slides. FISH of α-satellite and satellite III probes was performed under low stringency as previously described (Voullaire et al., 1993).

EXAMPLE 2

Somatic Cell Hybrids and Other Cell Lines

Skin fibroblasts and transformed lymphoblast cell lines were established from patient BE (Voullaire et al., 1993) and from his normal parents. The presence of the mardel 10 chromosome in the patient cell lines was confirmed by FISH. In addition to these cell lines, two somatic cell hybrids were produced by fusing cultured fibroblast cells derived from patient BE with the Chinese hamster ovary cell line CHO-K1 using polyethylene glycol. Hybrid cells were selected in a proline-free medium for the glutamic oxaloacetic transminase-1 (GOT-1) gene located in 10q24-q25 region. One of the hybrid cell lines, designated BE2C1-18-1f, was shown to contain the normal chromosome 10 but not the marker chromosome, while another hybrid cell line, designated BE2C1-18-5F, contained the marker chromosome but not the normal chromosome 10 of patient BE. The presence or absence of these chromosomes was established by karyotyping and ANTI-CEN/FISH probing. In addition, PCR analysis of an STS (sequence tagged site) marker, AFM259xg5, which resided in YAC-3, confirmed the status of these chromosomes in the hybrids and excluded the presence of submicroscopic fragments of the marker centromere region within the genome of BE2C1-18-1f, or the presence of the corresponding region of normal chromosome 10 within the genome of BE2C1-18-5f. Use of this STS marker also demonstrated that the mardel 10 chromosome has originated from the patient's father.

EXAMPLE 3

Antisera

Antiserum CREST #6 was from a patient with calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly and telangiectasia (a constellation of symptoms commonly referred to as “CREST”; Moroi et al., 1981; Fritzler and Kinsella, 1980; Brenner et al., 1981). Western blot analysis of this antiserum indicated that the primary antigens detected were human CENP-A and CENP-B. A specific anti-CENP-C polyclonal antibody, designated Am-C1, was produced by the inventors by expressing a partial mouse CENP-C polypeptide (amino acid #41 to 345) as a GST-fusion product in

E. coli

, followed by gel purification of the product and its use as an antigen for antibody production in rabbit.

EXAMPLE 4

Preparation of Standard Metaphase Chromosomes for FISH analysis

Actively replicating transformed lymphoblasts were incubated at 37° C. for 17 h in the presence of 0.1M final concentration of thymidine before they were centrifuged at 2000 rpm for 10 min, washed with pre-warmed RPMI, and incubated for a further 5-6h. 15 min before harvesting, colcemid (10 μg/ml) was added. Cells were harvested according to standard cytogenetic techniques using 0.075 M KCl hypotonic solution for 15 min at 37° C., followed by three fixative washes in ice cold methanol/acetic acid 3:1, dropped onto clean glass slides, and stored dessicated at −20° C. until required.

EXAMPLE 5

Preparation of Mechanically Stretched Chromosomes for ANTI-CEN/FISH Mapping

Method-I

This is an adaptation of the method described by Page et al. (1995). Colcemid (10 μg/ml) was added to actively dividing transformed lymphoblasts for 2-3 h, before the cells were centrifuged at 1500 rpm for 10 min, washed in PBS, and resuspended in 0.075M KCl hypotonic solution for 10 min at RT at a concentration of approximately 5×10

4

cells/ml; the use of fewer cells here gave better stretching of the chromosomes. 200-300 μl of this suspension were then cytocentrifuged onto clean microscope slides using a CYTOSPIN 2 (Shandon) at 1000 rpm for 5 min at high acceleration. The slides were immediately removed, placed flat in a shallow dish and very gently flooded with KCM (Potassium Chromosome Medium: 120 mM KCl, 20 mM Nacl, 10 mM Tris-HCl, 0.5 mM Na

2

EDTA, 0.1% v/v TRITON X-100) (Jeppesen et al., 1992). After 10 min at RT, immunofluorescence was performed without fixation (Earnshaw and Migeon, 1985; Earnshaw et al., 1989; Jeppesen et al., 1992; Jeppesen and Turner, 1993). KCM buffer was gently aspirated and 50 μl of CREST #6 serum [diluted 1:50 in 1×TEEN (1 mM Triethanolamine HCl, 0.2 mM Na

2

EDTA, 25 mM NaCl), 0.1% v/v TRITON X-100, 0.1% w/v BSA] was added to the cell area of the slide and covered with a parafilm coverslip. The slides were incubated for 30 min at 37° C., then washed very gently by flooding in 1×KB

−

[10 mM Tris-HCl (pH 7.7), 0.15M NaCl, 0.1% w/v BSA), three rinses of 3 min each at RT. The primary antibody was detected with Texas Red-conjugated Affini-pure Rabbit anti-Human IgG (H&L) (Jackson Laboratories) diluted 1:50 in 1×KB: 50 μl was added to each slide, covered with a parafilm coverslip, and incubated for 30 min at 37° C. The slides were again gently washed by flooding in 1×KB

−

for 2 min at RT, before they were fixed by flooding in 10% v/v formalin in KCM for 10 min at RT, followed by three rinses of 3 min each in distilled water. If FISH was not performed the slides were rinsed in PBS and mounted in DAP1 (0.25 μg/ml) in DABCO ANTIFADE mountant. [In experiments where CREST #6 and Am-Cl antisera were simultaneously used to label the centromere (FIGS.

2

B and C), the above procedure was followed except for the addition of Am—Cl diluted 1:100 together with CREST #6, and the Am—Cl antibody was detected using 1:100 diluted Donkey anti-Rabbit DTAF (Jackson Laboratories)].

If FISH was to be performed on the slides, they were then given a second fix in 3:1 methanol/acetic acid for 15 min at RT. The slides were air dried for at least 5 min and either processed for FISH or stored at −20° C. for up to several days before continuing. For FISH, the slides were dehydrated at RT in 70%, 90%, 100% v/v ethanol (2 min each) and air dried. Chromosomal DNA was denatured in deionised 70% v/v formamide/2×SSC, pH 7.0 at 82° C. for 8 min followed by immediate dehydration in 70%, 90% and 100% v/v ethanol at −20° C. for 2 min each, then air dried for at least 10 min. (This high temperature of denaturation was critical to obtain maximum FISH signals). An amount of 15 μl of the prepared probe was added to each slide, covered with a 22 mm

2

coverslip, and sealed with rubber cement. Slides were hybridized overnight in a humid chamber at 37° C., then rinsed in 2×SSC at RT, followed by 3 washes of 0.1×SSC at 60° C. for 5 min each, rinsed again in 2×SSC, and immersed in a blocking agent of 5% non fat milk in 4×SSC for 10 min at RT. Probe hybridization was detected by incubation with FITC-conjugated avidin at 37° C. for 30 min, followed by three washes of 5 min each at RT in wash buffer (4×SSC, 0.05% v/v Tween-20). Signals were amplified by incubating with goat anti-avidin D antibodies for 20 min at 37° C., followed by three washes of 5 min each at RT in wash buffer, then with another layer of avidin-FITC for 30 min at 37° C., before the slides were washed in wash buffer, rinsed in PBS, and counter-stained with DAP1 (0.25 μg/ml) in DABCO mountant.

Method-II

The following method was modified from that of Haaf and Ward, (1994). Actively dividing lymphoblast cells were treated with 10 μg/ml colcemid for 2-3 h, washed in PBS and resuspended in a hyptotonic solution consisting of 10 mM Hepes (pH 7.3), 30 mM glycerol, 1.0 mM CaCl

2

and 0.8 mM MgCl

2

, at a cell density of approx. 2.5×10

2

/ml. After 10 min of hypotonic treatment at RT, 300 μl were cytocentrifuged (Shandon—CYTOSPIN 2) onto glass slides at 800 rpm for 4 min. The slides were immediately removed from the centrifuge, dried for 15 sec, fixed in methanol at −20° C. for 20-30 min, rinsed in acetone at −20° C. for a few sec, then washed in 3 rinses of PBS at RT. Immunofluorescence staining was done using CREST #6 at a dilution of 1:50 in PBS. After incubation at 37° C. for 30 min, the slides were washed three times in PBS for 2 min each. This primary antibody was then detected by a further incubation for 30 min at 37° C. with Texas Red-conjugated Rabbit anti-Human IgG diluted at 1:50 in PBS. The slides were fixed in 10% v/v formalin in KCM for 10 min at RT, then washed in 3 rinses of distilled water and drained. Before FISH was performed, slides were fixed in methanol/acetic acid 3:1 for 15 min at RT and air dried. Chromosomal DNA was denatured in 70% v/v deionised formamide (pH 7.0) in 2×SSC at 82° C. for 4-6 min. After dehydration in an ice cold ethanol series the slides were air dried, and used for FISH as described for Method I. Slides could be stored covered in foil at RT after methanol/acetic acid fix for up to several weeks before FISH.

Both methods I and II were used to obtain the results shown in

FIGS. 2B

,

2

C,

3

and

4

B.

EXAMPLE 6

Image Analysis

Hybridization signals for YAC mapping on standard metaphase preparations utilized a normal fluorescence microscope. Images for the ANTI-CEN/FISH experiments were analyzed on a Zeiss AXIOLAB fluorescence microscope equipped with a 100× objective and a cooled CCD camera (Photometrics Image Point) controlled by a POWER MAC computer. Gray scale images were captured separately using a LUDL filter wheel and controller for Texas Red, FITC and DAPI. These images were pseudocoloured and merged using IPLAB Spectrum software from Signal Analytics Corporation. A number of difficulties were commonly associated with the ANTI-CEN/FISH technique: (a) the deliberate “stretching” of the chromosomes, whilst increasing the resolution of mapping sometimes caused serious distortion to the chromosomes, often making them quite dysmorphic; (b) FISH treatment following the ANTI-CEN-labelling often significantly reduced the ANTI-CEN signals; (c) more highly stretched chromosomes (which would potentially give better mapping resolution) generally gave weaker ANTI-CEN signals; and (d) the ANTI-CEN signal on the mardel 10 centromere was usually weaker than those of the other human chromosomes. Thus, a cell would only be considered informative and used for scoring if both the p′- and q′-arms of the mardel 10 chromosome were discernible and separated by a discrete ANTI-CEN signal. In addition, FISH signals for both the test probe and the 10pC38 cosmid tag (used to identify the q′-arm of, and thus orientate, the marker chromosome) must be clearly present. Using these criteria, the overall frequency of informative cells was found to be approximately 1 in every 20-30 metaphases analyzed.

EXAMPLE 7

Restriction Analysis of Patient DNA

High-molecular weight genomic DNA was extracted from cultured fibroblast cell lines of patient BE and those of his parents and digested with different enzymes to generate restriction fragments ranging from <1 kb up to ˜1 Mb. The digested DNA was resolved either on a standard agarose gel or by pulsed-field gel electrophoresis (PFGE) using a Bio-Rad CHEF-XA Mapper. For filter hybridization, 50-100 ng of whole cosmid or PAC DNA was labelled by random priming. The labelled probe was then added to 2 ml of hybridization buffer (0.5M Na

3

HPO

4

, 7% w/v SDS, 1% w/v BSA, 1 mM EDTA, pH. 7.0) containing 500 μg of human placental DNA (Sigma). The mixture was boiled for 5 min, then placed in a 65° C. water bath for preannealing of repetitive DNA for 90 min. The preannealed probe mix was then added to prehybridizing filters and hybridized overnight at 65° C. Post-hybridization washes were at a final stringency of 0.1×SSC, 0.1% w/v SDS at 68° C.

EXAMPLE 8

Identification of a YAC region spanning the marker centromere

The initial search for DNA sequences spanning the centromere of the mardel 10 chromosome was based on fluorescence in situ hybridization (FISH) of existing cosmid and YAC clones (Moir et al., 1994; Zheng et al., 1994) that have been mapped to the q24-q26 region of the normal human chromosome 10 where the new marker centromere was formed (Voullaire et al., 1993) (FIG.

1

A). This search led to the identification of a 4 megabase YAC contig (designated #082) that spanned the marker centromere region (FIG.

1

B).

FIG. 1C

graphically presents the FISH mapping results with selected YACs from this contig. As can be seen, two of the YACs (YACs-1 and YAC-2) mapped to the q′-side of the marker centromere, whereas the remaining YACs mapped to the p′-side of the centromere. The low signal level observed for YAC-3 was due to a large proportion f this probe hybridising directly on the centromere itself. These results, therefore, provided evidence that YAC contig #082 spanned the marker centromere, and that the centromere region was likely to be within YAC-3, where the “cross-over” between the q′ and p′ signals occurred.

EXAMPLE 9

Development of Improved ANTI-CEN/FISH Methods for the Simultaneous Detection of Marker Centromere and Single-copy Cosmid DNA Probes

Although normal fluorescence microscopy and FISH analysis of standard metaphase chromosomes were adequate for the initial identification of the YAC contig spanning the marker centromere, methods with significantly higher sensitivity and resolution were needed to allow further walking into the marker centromere DNA. Three requirements have to be satisfied by these methods: (a) the metaphase chromosomes have to be extended to offer much greater mapping resolution, (b) the centromeres have to be more precisely defined than that offered by a cytogenetic constriction, and (c) the methods should allow simultaneous visualization of both the centromere antibody and FISH signal. Two published methods were explored (designated here as ANTI-CEN/FISH methods) based on extending metaphase chromosomes by mechanical stretching and labelling of the neocentromere by autoimmune antibodies (Haaf and Ward, 1994; Page et al., 1995). Since these methods were originally established for the labelling of normal centromeres and for FISH analysis of highly repeated DNA, they were modified (see Example 4) to allow detection of the generally reduced ANTI-CEN signal of the subject marker neocentromere and the lower FISH signals resulting from the use of single-copy cosmid DNA probes.

With the improved detection methods, the status of α-satellite and satellite III DNA on the marker neocentromere was reassessed, since this was previously determined using standard microscopy and FISH (Voullaire et al., 1993).

FIG. 2A

shows the result of antibody labelling using CREST #6 and FISH using α-satellite DNA, and indicated the absence of detectable signal on the marker centromere. The same result was obtained when the experiments were repeated without ANTI-CEN-labelling, ruling out the possibility that the anti-centromere antibody might have obscured any weak FISH signals. Similar results were obtained with satellite III DNA. Since in separate reconstruction experiments, it was possible to demonstrate the sensitivity of the procedure in detecting a single-copy DNA probe of less than 1.5 kb, and making the reasonable assumption that the low-stringency hybridization conditions used for the α-satellite and satellite III DNA which, by virtue of the use of >100-fold excess of probes and the strong hybridisation of these probes to all the other centromeres, would have allowed the detection of any related sequences, it can be concluded that these satellite are absent.

EXAMPLE 10

Co-localization of CENP-C and CENP-A on the Marker neocentromere

To test if CENP-C is present on the marker centromere, a specific rabbit polyclonal antibody was prepared against a recombinant product of mouse CENP-C. This antibody, designated Am—Cl, reacted strongly with the centromeres of rodent and human chromosomes.

FIG. 2B

shows results for the labelling of stretched human metaphase chromosomes using this antibody simultaneously with the CREST #6 autoimmune antibody. As can be seen, irrespective of the degree of chromosome stretching, the signals for the two antibodies coincided fully on all the centromeres. The localization of these two antibodies on the marker chromosome was further determined by employing the 10pC38 cosmid tag in an ANTI-CEN/FISH experiment to identify the marker chromosome. The results indicated that both the antibody signals were clearly present and again coincided completely on the marker centromere (

FIG. 2C

, a-e). Although CREST #6 was known to bind CENP-A and CENP-B, indirect evidence suggests that binding to the marker centromere presumably occurred via CENP-A since the presence of the marker centromere was previously demonstrated not to bind CENP-B (Voullaire et al., 1993). The above results, therefore, established the localization of CENP-C, and probably CENP-A, on the marker centromere.

EXAMPLE 11

Localization of the anti-centromere antibody-binding domain

For further walking into the marker centromere region, cosmid libraries were prepared from total yeast genomic DNA containing YACs-2, -3, -4, -6, -7, -13, and -17. Cosmid clones containing human DNA inserts were isolated by hybridization with human COT-1 DNA using low stringency. All resulting cosmids were screened by standard FISH to confirm their localization to the expected marker centromere and normal chromosome 10 regions, and to eliminate clones that might have originated from other genomic sites due to chimeric YACs. Positive clones were then analyzed further with the ANTI-CEN/FISH methods, using CREST #6 to label the centromere.

FIG. 3

a

(I and II) show examples of cosmid signals that mapped to the q′- and p′-side, respectively, of the marker centromere in the ANTI-CEN/FISH experiments. The cosmid tag (clone 10pC38) was used in these experiments to define the q′ arm of the marker chromosome. For cosmid walking, we concentrated on clones derived from YAC-3 since FISH mapping of YAC contig #082 indicated that the marker centromere region was likely to be within this YAC.

FIG. 4

a

shows a restriction map of the region covered by this and surrounding YACs and compares this map with a genomic map derived from patient BE. The relative positions of a series of cosmid clones (including five independent PACs) were also determined and placed on the YAC map.

FIG. 4

b

presents the ANTI-CEN/FISH results obtained with a number of the cosmid clones and one of the PAC clones. Clones Y3C64, Y6C8, and Y3C94 localized preferentially to the q′-side, while Y13Cl+C8 and Y17C6 localized preferentially to the p′-side of the marker centromere, suggesting that the nucleus of the antibody-binding domain is situated between these two cosmid clusters. Within this central region a group of cosmid clones comprising the HC-contig (

FIG. 4

a

) was found to map closely around the ANTI-CEN signal.

FIG. 4

c

shows a restriction map for eight different overlapping clones from this HC-contig. The chromosomal positions of five of these overlapping clones were analyzed in detail using ANTI-CEN/FISH.

FIG. 4

b

shows the cumulative results for more than 60 informative chromosomes for each of these five probes. The results indicated that Y7C14 mapped preferentially q′- of the antibody-binding domain, while the remaining four clones (Y4C45, Y6C10, Y6C21 and Y3C3) mapped preferentially to the p′-side. In addition, the results for PAC5 (a 75 kb-insert PAC clone that overlapped with the p′-end of PAC4 by approximately 5 kb; see

FIG. 4

a

) provided further evidence for the emergence of the HC-contig region onto the p′-arm. Based on these results, we conclude that the eight contiguous cosmid clones within the HC-contig shown in

FIG. 4

c

, which together constitute ˜80 kbp of DNA, have defined the nucleus of the antibody-binding domain of the marker centromere.

From the above ANTI-CEN/FISH results, it was difficult to determine if the sequences of the HC-contig and its surrounding DNA, both originally derived from a normal individual, were part of the marker centromere DNA, or whether these sequences simply flanked a transposed centromere DNA with an unrelated nucleotide composition. However, supporting evidence from the ANTI-CEN/FISH experiments suggested that the DNA of the HC-contig region appeared to be a part of the marker centromere. This came from the mapping of Y6C10 and Y6C21 onto superstretched chromosomes that were occasionally detected in the slide preparations. An example of such mapping is shown in

FIG. 3

b

using Y6C21. As can be seen, whilst a significant portion of Y6C21 hybridized to the p′-side of the CREST signal on the highly extended chromosome, a substantial portion of the cosmid DNA also overlapped directly with the CREST signal. This suggests that at least part of the HC-contig region actually comprises the same DNA sequence as the marker centromere. This possibility was further investigated by detailed genomic mapping.

EXAMPLE 12

The Marker Centromere DNA has a Similar or Identical Sequence Organization as the HC-Contig

The genomic organization of the HC-contig region was compared with that of the corresponding DNA region of the mardel (10) chromosome. Three overlapping cosmids (Y7C14, Y6C10, and Y4C7, the latter being essentially the same as Y6C21;

FIG. 4C

) from the HC-contig were used as probes to analyze the restriction patterns of genomic DNA prepared from patient BE and those of this karyotypically normal parents.

FIG. 5

shows examples of the band patterns obtained with Y6C10, while Table 1 summarizes the results for all the enzymes tested with Y7C14, Y6C10 and Y4C7. The detection of a single and on PFGE gels with a number of the enzymes indicated that the cosmid DNA sequences were unique within the human genome (SfiI, SalI, KspI, KpnI and BclI in

FIG. 5A

; Table 1). The detection of a single on PFGE gels with a number of the enzymes (ClaI in

FIG. 5A

; Table 1) could be explained by differential methylation of different restriction sites found in this region (Nelson and McClelland, 1991); the reproducibility of these multiple band patterns ruled out incomplete digestion as a possible cause. The multiple bands detected with the more frequent cutting enzymes on a standard gel (FIG.

5

B and Table 1) were a result of the presence of cleavage sites present within the probe DNA, since similarly digested cosmid DNA electrophoresed next to the genomic DNA yielded identical patterns for all the bands not containing cosmid vector sequences. In all, 37 enzymes were used to generate more than 160 different fragments for the three cosmid probes (Table 1). The results indicated that, except for a polymorphic fragment found in one of the parents, an identical banding pattern was present in the genomic DNA of patient BE and those of his parents. Furthermore, when the restriction patterns obtained for the genomic DNA of patient BE were compared with those of the smatic hybrid cell line BE2C1-18-5F, which contained the marker chromosome but not the normal chromosome 10, no detectable difference was seen between the two DNA preparations within the HC-contig region (FIG.

5

C).

In addition to Y7C14, Y6C10and Y4C7, a host of other probes from within or surrounding the HC-contig have been tested, each with an average of 12 different information enzymes. These probes included PAC4 (which spanned the entire HC-coding region shown in FIG.

4

C), Y3C64, Y3C109, Y6C6, Y6C8, Y3C94, PAC1, Y3C90, Y4C4, Y4C8, Y4C13, and Y3C33. The results again indicated identical restriction enzyme patterns between patient BE and normal DNA. Thus, through the analysis of a relatively large number of probes covering about 500 kb of YAC-3 around the HC-contig region, and the use of a high density of restriction enzymes that generated a range of fragments from <1 kb to −1 Mb, it was evident that the market centromere DNA and a substantial stretch of its adjoining regions showed no detectable difference against the corresponding genomic region of the normal chromosome 10.

Since a potential limitation of the above Southern blot analyses was that highly repeated sequences were not detected because of the preannealing step used in the hybridisation procedure, a different approach was employed to compare the DNA of the marker chromosome and that of the normal chromosome 10. In this approach, oligonucleotide primers from different regions of the HC-contig were used to prepare a series of PCR fragments from the BE2C1-18-5F and BE2C1-18-1F hybrid cell lines. Electrophoretic comparison of such fragments, which randomly covered approximately 40 kb of the HC-contig, indicated no detectable difference between the two chromosomes and provided independent support for the results obtained in the Southern blot analyses. Thus, it can be concluded that the sequence organization of the marker centromere region is similar, if not identical, to that found in the HC-contig region of the normal chromosome 10.

EXAMPLE 13

Implications for Centromere Study and Mammalian Artificial Chromosome Construction

The mammalian centromere has been difficult to study due to the massive amount of repetitive DNA normally associated with it. By avoiding such repetitive DNA and analyzing the unusual centromere found in the present marker chromosome, the inventors have created a much more tractable system for centromere studies. The present analysis has already shed some light on the important question of DNA sequence versus conformational requirement of a centromere, and on the intriguing concepts of latent centromeres and epigenetic mechanisms. One urgent application of this DNA is to use it to identify the primary protein(s) which binds to the centromeric DNA. Another important application of the marker centromere DNA is in the construction of mammalian artificial chromosomes. Such artificial chromosomes offer a potentially powerful vehicle for the structural and functional analysis of chromosomes, for the genetic manipulation of plants and animals, and for the stable transmission of therapeutic genes in human gene therapy. The artificial chromosomes require a functional mammalian centromere, and the marker centromere DNA element of the present invention now provides a suitable centromere especially because of its relatively small size in the absence of α-satellite DNA and its cloning stability, as indicated by the cosmid, YAK and BAC clones of the HC-contig and NC-contig.

EXAMPLE 14

Sequence Analysis

FIGS. 6

,

16

A and

16

B show partial nucleotide sequences for the HC-contig (SEQ ID NO: 3) NC-contig [SEQ ID NO: 4] and F2 (BAC/F2-14) [SEQ ID NO: 5-29] regions, respectively.

EXAMPLE 15

Human Artificial Chromosome (HAC)

The following are examples of the different approaches being used in the inventors' laboratory for the production of a HAC:

Retrofitting of HC-contig DNA from normal chromosome 10

This procedure aims to produce HACs of 100 kb to >1 Mb using the region of the normal chromosome 10 containing and surrounding the HC-contig DNA. The generation of a HAC by this approach will provide crucial proof that this normal DNA region can be reactivated to form a functional centromere.

A retrofitting procedure suitable for introducing human telomeres to both ends of any YAC prepared in the pYAC4 vector in the yeast host strain AB1380 has been previously described (Larin et al., 1994; Taylor et al., 1994, 1996). YACs (in particular YAC-3 and YAC-5) spanning the normal HC-contig region are used for retrofitting by plasmid constructs designed to recombine with their pYAC4 vector arms (FIG.

7

). The construct pLGTEL 1 is used to target the left arms of the YACs. This serves to add a LYS2 yeast selectable marker, gpt element for ultimate selection in mammalian and avian cell culture, and a human telomere. The right arm of the YACs are targeted by homologous recombination with pRANT 11 to produce a final construct where additional markers are introduced along with a second human telomere to cap the construct. Specifically, an ADE2 yeast marker is added and the URA3 gene of the YAC is disrupted, serving a useful role in negative selection of the construct. A neomycin (neo) resistance gene shown to function in mammalian and avian cells is also introduced. The finished constructs are transfected into different cultured cell lines, including HT1080 (of human sarcoma origin) (Larin et al., 1994; Rasheed et al., 1974), DT40 (a recombination-proficient chicken cell line) (Dicken et al., 1996), and BE2CI-18-5f (a human/hamster somatic hybrid cell line containing the mardel (10) chromosome but not the normal chromosome 10).

In vitro cloning HC-region into YAC/HAC vectors

The different vectors used for the cloning of the normal and mardel (10) centromeric DNA in the preparation of HACs are summarised in Table 2.

A number of different YAC cloning strategies are employed:

Conventional YAC cloning approach.

FIGS. 8A-D

show the different vectors used for cloning DNA as YACs by the conventional restriction/ligation methods. These YACs can then be shuttled into mammalian cells and tested for HAC function.

ALU-ALU circular TAR cloning approach. Transformation-associated recombination (TAR) in the yeast

S. cerevisiae,

is a method for constructing linear and circular YACs from mammalian DNA (Larionov et al., 1996b). The recombination process is shown in FIG.

9

. Briefly, the technique involves the use of a vector (pVC39-AAH2,

FIG. 8E

) lacking an autonomous replicating sequence (ARS) but containing a functional yeast centromere (e.g. CEN6) and selectable marker (e.g. HIS3), and two ALU DNA hooks to trap mammalian DNA by recombination at ALU sequences after co-transformation of linearized vector and high molecular weight DNA into yeast spheroplasts and followed by selection on medium lacking histidine. The key to the process is that the mammalian DNA provides an ARS (11-bp sequence found frequently in mammalian DNA) which allows the HIS*/CEN vector to replicate as a circular YAC. These YACs are very stable and range in size from 100 kb to greater than 600 kb (Larionov et al., 1996b).

pVC39-AAH2 vector is used to clone DNA from hybrid BE2CI-18-5f to make YACs with an average insert of 250 kb. This TAR vector is further modified to create pAAH-TCNa (

FIG. 8G

) so that it has the ability to shuttle between yeast and mammalian cells (as outlined in FIG.

10

), including the potential to expose human telomeres (TEL) at each end of a cloned fragment using a unique restriction site I-SceI.

Semi-specific and specific circular TAR. A modified circular TAR method utilising two specific 5′C and 3′C DNA hooks (300-700 bp in size) may be used to clone a specific human DNA at a frequency of 3/1000 HIS* transformants. The inventors prepared the vectors pVC39-ALU/C3-F2(+/−) and pTCN-TCS (Table 2) to perform semi-specific and specific TAR cloning, respectively.

The Semi-specific TAR methodology is a modification of a specific circular TAR strategy which permits the site directed isolation of target chromosomal DNA. Furthermore, in accordance with the present invention, the methodology described herein enables the site-specific cloning of target chromosomal DNA from total genomic DNA as a circular YAC at relatively high frequencies and without the need for the construction and extensive screen of complex libraries made from genomic DNA.

In a preferred embodiment of the present invention, the methodology employs a single specific DNA hook which flanks the mardel (10) chromosome and a less specific Alu-hook to trap the other side of the target DNA.

In initial experiments, a unique repeat DNA-free, 1.4 kb EcoRI fragment (designated C3-F2) was identified from the p′ side of the 80-kb HC-contig (

FIG. 11A

) (du Sart et al., 1997). This fragment was subcloned into the centromere-based yeast circular TAR vector, pVC39-AAH2, by replacing the existing BLUR13 Alu (Larionov et al., 1996b) to create the pVC39-ALU/C3-F2 constructs. As the specific orientation of the C3-F2 sequence on the chromosome was not known, the fragment was cloned in two different orientations, for which the (+) orientation (

FIG. 11B

) was expected to trap the genomic region to the left of C3-F2, while the (−) orientation was expected to trap the region to the right. Both constructs were used in yeast transformation.

As a source of genomic DNA containing the neo-centromere, a somatic hybrid cell line, BE2C1-18-5f (du Sart et al., 1997), containing the mardel 10 chromosome but no the normal human chromosome 10 was used, 5 μg of high-molecular-weight DNA from this cell line and 1 μg of pVC39-ALU/C3-F2(+) or pVC39-Alu/C3-F2(−) (linearized with SmaI to expose the 0.21-kb Alu and 1.4-kb C3-F2 hooks) were co-transformed into 10

9

(previously prepared and stored frozen) spheroplasts of

S. cerevisiae

YPH857 which carries a HIS3 gene deletion, (Sikorski and Hieter, 1989) and grown on SD, without HIS medium, (Larionov et al., 1996a;b) to yield between 10 and 100 HIS* colonies. Control experiments in which YPH857 was transformed with vector alone did not produce any colonies, indicating that the C3-F2 fragment lacked ARS-like sequences. Twenty TAR experiments were performed and HIS* colonies were picked into 96-well trays containing YPD medium (supplemented with 50 μg/ml ampicillin and 15 μg/ml tetracycline), grown at 30° C. with aeration for 24 h and stored in 20% (v/v) glycerol at −70° C. Total yeast DNA was prepared in pools of 48 (Kwiatkowski jr et al., 1990) and screened by PCR with the primers norm 5 and norm 7 (Table 3) which are located 30-kb q′ of C3-F2 (FIG.

11

A). Two desired positive clones, designated 5f-52-E8 and 5f-38-F2, which contained the neo-centromere DNA derived from mardel 10 and mardel (10) and the DNA immediately p′ of the neocentromeric DNA, respectively, were identified. For subsequent studies, these clones were grown on SD without HIS medium and single colonies were re-isolated for characterization.

Initially, the sequence nature and sizes of the 5f-52-E8 and 5f-38-F2 insert DNA were determined. High molecular-weight DNA was prepared in agarose blocks and digested with an enzyme (SrfI) that linearized with YAC (FIG.

11

A). The linearized DNA, as well as uncut intact DNA, were resolved by pulsed-field gel electrophoresis (PFGE), transferred onto a nylon membrane and probed with radiolabelled PAC4, a P1-derived artificial chromosome clone containing a 120-kb insert that spans the entire HC-contig from normal chromosome 10, (du Sart et al., 1997) following preannealing with human placental DNA to suppress repetitive DNA. The intact 5f-52-E8 and 5f-38-F2 remained trapped in the electrophoretic wells and the linearized DNA migrated into the gel and demonstrated a size of approximately 110 kbp and 80 kbp, suggesting insert sizes of about 105 kbp and 75 kbp, respectively (given that the vector size is 5.9 kb).

Despite the use of a genomic DNA source previously shown by sequence-tag-site (STS) analysis to be free from normal chromosome 10 material, it is desirable to independently confirm the mardel (10)-origin of the 5f-52-E8 YAC clone. This was achieved using a set of primers (norm 17 and 18;

FIG. 11A

) that detected a variable-number-tandem repeat (VNTR) region within the HC-contig/neocentromere region. The results clearly indicated the presence of a 1.4-kb PCR product that was specific for the mardel (10) chromosome (Table 3).

PCR was used to further compare the 5f-52-E8 DNA with the previously cloned HC-contig sequence derived from normal chromosome 10. PCR products with sizes ranging between 0.2 and 15.9 kb were generated by standard PCR or with the Expand Long Template PCR system (Boehringer-Manneheim). Products greater than 1 kb were digested with frequent cutting enzymes, RsaI and BsiXI, and their fingerprints were compared by agarose gel electrophoresis. The results, shown in Table 3, indicated the absence of any detectable difference between the 5f-52-E8 DNA and those of the corresponding regions of the normal chromosome 10 (in somatic cell hybrid BE2C1-18-1f) and the neocentromere region of mardel (10) (in somatic cell hybrid BE2C1-18-5f). These results also demonstrated that the YAC 5f-52-E8 spanned at least 75 kb of the HC-contig region (FIG.

11

C), consistent with the size determined by PFGE. Furthermore, the ability of all the internal primers to amplify DNA from 5f-52-E8 strongly suggested that the YAC was not chimeric. This result was confirmed by isolating DNA from four single-colony isolates of 5f-52-E8, digesting these with EcoRI and EcoRV, and probing with radiolabelled PC4. The hybridization patterns obtained with these enzymes were consistent with those established in the previous study (du Sart et al., 1997). Thus, this analysis, based on cloned DNA derived directly from mardel 10, has provided confirmation that the neocentromere DNA region is structurally identical to that of the corresponding HC-contig region of the normal chromosome 10 (du Sart et al., 1997).

The circular YACs 5f-52-E8 and 5f-38-F2 were further retrofitted with the yeast-bacterial-mammalian cells shuttle vector BRV1 as previously described (Larionov et al., 1997). The resulting BAC clones were designated BAC/E8-1 and BAC/F2-14, respectively (FIG.

11

D).

The specific TAR strategy is outlined in FIG.

12

and uses unique fragments from the HC-contig region, such as the ends of PAC4 (a 120 kb-insert PAC clone containing the HC-region) to create the YAC/HAC shuttle vector pTCN-TCS. An example of a YAC/HAC construct containing the HC-contig region of normal chromosome 10 is shown in FIG.

13

.

Completed constructs are transfected into different cultured mammalian or chicken cells (see above) by lipofection using Transfectam or DOSPER.

In vivo “cloning” of HC-region into HAC vectors

This strategy employs a technique known as Telomere Associated Chromosomal Truncation (TACT) (FIG.

14

). The technique is based on the principle that cloned mammalian telomeric DNA when reintroduced into a mammalian cell can seed the formation of a new telomere at an intrachromosomal location. If the introduced telomeric DNA is target to a known site through homologous recombination, integration at that location and subsequent truncation of distal sequences on the original chromomosomal arm can result (Brown et al., 1994; Farr et al., 1995). This technique is employed in our own study to truncate the mardel 10 chromosome on either side of the HC-contig/core centromeric DNA element to produce in vivo a stable HAC of minimal size.

FIG. 15A

shows an example of TACT-construct used in our study. Key features of this construct are: (a) Cloning of the pericentric human genomic DNA in both orientations (+/−). This is necessary since we do not know the chromosomal orientation of this DNA. This DNA is used to target the human telomeric sequences to locations on either side of the HC-coding region on mardel 10. Genomic DNA is derived from several different sources including Y2C24, Y3C64, Y3C109, Y3C94, Y13C12, Y13C15, Y17C6, Y17C8. The resulting truncation derivatives produced using these genomic DNAs will vary in size accordingly. (b) The termini contain 2.4 kilobases of tandem repeat human telomeric DNA (htel). This DNA has been shown previously to act as a substrate for mammalian telomerase to allow seeding of a complete telomere tens of kilobases in length. (c) The hygromycin (Hyg) resistance gene allows for positive selection of mammalian cell lines containing sequences integrated into the genome. This is the initial screening procedure. In addition, some constructs contain the neomycin phosophotransferase gene (Neo) rather than Hyg. (c) The Herpes simplex the thymidine kinase (TK) gene is used for negative selection against non homologous integration events into the genome. Those cell lines containing the TK gene can be selected against by adding the nucleoside analogue gancyclovir.

FIG. 15B

shows another example of TACT-construct used in our study. In addition to the features of the linearised construct shown in

FIG. 15A

, specific additional features are: (a) The incorporation of tandem telomeric blocks (htel.htel) since others have shown these to have the highest seeding efficiency of new telomeres in mammalian cells. (b) The incorporation of yeast selectable marker (eg. URA3), DNA origin of replication (eg. ARS), and centromere (eg. CEN6), to allow transfer and maintenance of the resulting truncation derivatives into yeast. This should facilitate further characterisation and manipulation, such as the introduction of therapeutic genes for gene therapy purposes. (c) The relocation of the TK gene adjacent to the genomic DNA to increase the effectiveness of the negative selection system. (d) The human growth hormone (GH) gene has been included to allow proof of principle that human genes can be introduced into a HAC and expressed under the control of endogenous regulatory elements. This is essential for gene therapy applications of the resulting HAC. (e) A CMV promoter upstream of a P1 phage 1oxP site (CMV/1oxP) has been included to allow introduction of large human genes into a HAC in vivo. A plasmid containing a gene of interest, a second 1oxP site and a promoterless selectable marker gene is introduced into a mammalian cell line containing the HAC. Transient expression of CRE recombinase results in recombination between the two 1oxP sites within the cell, thereby integrating the introduced plasmid into the HAC and placing the selectable marker gene next to the CMV promoter to allow for marker selection.

For chromosomal truncation, the above TACT-constructs are transfected into a somatic cell hybrid line BE2CI-18-5f containing the mardel (10) chromosome. Positive selection is applied for Hygromycin or Geneticin resistance whereas negative selection is applied against the Thymidine Kinase Gene. Resulting colonies are further screened with distal p′ and q′ DNA fragments to ascertain the presence of absence of the two mardel 10 chromosome arms. In addition to the BE2CI-18-5f cell line, a human/chicken somatic cell hybrid line (derived from the recombination-proficient DT40 chicken cell line; Dieken et al., 1996) containing the mardel (10) chromosome will also be generated and used.

EXAMPLE 16

Analysis of HAC

Irrespective of which of the approaches described above is used, the presence of a new product in a mammalian cell line as an extrachromosomal, artificial chromosome, will be assessed by fluorescence in situ hybridisation (FISH) analysis, as well as tested by extracting high molecular weight DNA to determine independently existing chromosomal entity on pulsed field gel. The stability of the construct through successive cell division, both in the presence and absence of drug-resistance selection, will be determined. The presence of the construct, in all or a high percentage of the original transfected cells indicates stability. Demonstration of this stability indicates the successful creation of a HAC.

EXAMPLE 17

Production of HAC

This example describes the use of the neocentromere as a source of centromeric DNA in the “bottom-up” approach to produce HACs in human cell culture. Bacterial artificial chromosomes (BACs) containing cloned neocentromeric DNA and a selectable marker were co-transfected with human telomeric DNA into human HT1080 cells to yield independent HACs that were single-copy and stable in the absence of selection. The properties of these HACs, and their potential utility as a new, improved vector system for gene therapy are described.

Experimental Protocol

Preparation of DNA. Highly-purified BAC DNA was prepared using Qiagen columns according to the manufacturer's instructions. Prior to transfection, BACs were linearized with SgrAI in the presence of 2.5 mM spermidine and examined by pulsed-field gel electrophoresis. Human telomeric DNA was gel-purified as a 1.6-kb BamHI/BglII fragment from pSXneo270T2AG3 (Bianchi et al., 1997). High-molecular-weight genomic DNA was prepared from cultured cell lines using standard methods (du Sart et al., 1997).

Transfection of HT1080 cells. Transfection of human fibrosarcoma cell line HT1080 (Rasheed et al., 1974) was performed using the DOPSER liposomal transfection reagent (Boehringer-Mannheim). The day before transfection, 6-well trays (each well is 962 mm

2

) were seeded with 3×10

5

HT1080 cells per well and grown at 37° C., 5% CO

2

. Different combinations containing 1-2 μg of each BAC, 50 ng of telomeric DNA, 100 ng of each PAC-1, 4 and 5(du Sart et al., 1997) and 50 ng of human genomic DNA were prepared in 50 μl of HBS (20 mM HEPES, 150 mM NaCl) supplemented with 0.075 mM spermidine and 0.030 mM spermine. These DNA cocktails were mixed with 50 μl of 0.4 μg/μl DOPSER (diluted in HBS) and left at room temperature for 15 to 20 min. The HT1080 cells were washed with PBS (phosphate buffered saline) and 1 ml of serum-free DMEM (Dulbecco's modified Eagles medium) was placed in each well. The DNA-DOPSER mixture was then added dropwise with swirling and the cells were incubated for 6 h. 1 ml of DMEM and 20% v/v fetal calf serum (FCS) was then added and the cells left for 24 h at 37° C., 5% v/v CO

2

. The cells were harvested and seeded into 48-well cluster trays (each well is 100 mm

2

) containing DMEM-10% v/v FCS supplemented with Geneticin (G418, Gibco-BRL) at 250 μg/ml. The media was changed every 3 to 4 days. G418-resistant colonies normally appeared 10 to 14 days after transfection. These colonies were expanded into duplicate 6-well trays, where the cells of one tray were stored frozen in liquid N

2

, and the remaining cells were analysed by fluorescence in situ hybridization (FISH).

Cell culture and mitotic stability. HT1080 cells were grown in DMEM supplemented with 10% v/v FCS, penicillin/streptomycin, and glutamine. The mitotic stability of HAC containing clones was determined by growth in 25 cm

2

flasks in the presence (200-250 μg/ml) or absence of G418 selection, and grown to confluency (3-4 days) and split 1/5 and 1/10, respectively. Aliquots of each culture were harvested fortnightly and analysed by FISH (20-50 metaphases) with BAC/E8 and/or BAC/F2 probes.

FISH, ANTI-CEN/FISH and PRINS/FISH. Fluorescence in situ hybridization (FISH) analysis of HT1080 clones was performed with BAC/E8, BAC/F2, and/or α-satellite DNA probes. Hybridization using the BAC probes were performed under high stringency whereas the α-satellite DNA probes were used in low stringency conditions (du Sart et al., 1997). ANTI-CEN/FISH analyses involved an initial immunofluorescence staining step using a CREST antibody or specific antibodies against CENP-B, CENP-C, or CENP-E, followed by FISH using the probes described above, essentially as previously described (du Sart et al., 1997).

Results

HAC construction strategy. The basic strategy involved the co-transfection of the 10q25.2 neocentromere DNA with human telomeric DNA into human cells. The neocentromere region is cloned as two, circular YACs in

Saccharomyces cerevisiae.

To facilitate handling and purification of the cloned DNA in large quantities, these YACs are retrofitted into BACs and maintained episomally in

E. coli

as circular molecules. One of the BAC clones, BAC/E8, is 120 kb in size and has an insert of 105 kb that encompassed 70 kb of the 80-kb core NC-DNA region (FIG.

16

). The second BAC clone, BAC/F2, has an insert size of 75 kb that overlapped BAC/E8 by 1.4 kb, and contains ˜10 kb of the core NC-DNA while extending ˜65 kb into the ′-side of the mardel (10) chromosome (FIG.

16

). The BAC vector backbone further contains the neomycin-resistance (Neo®) gene to allow selection in mammalian cells. BAC/E8 and BAC/F2, used either on their own, in combination with each other or with additional DNA are used in the following transfection experiments.

Transfection of HT1080 cells. The human cell line HT1080 (Rasheed et al., 1974) is chosen for the transfection experiments because of its near-diploid karyotype, its high level of telomerase activity (Holt et al., 1997), and its demonstrated ability to form microchromosomes containing de novo centromeres from transfected arrays of α-satellite DNA and human telomeric DNA (Harrington et al., 1997; Ikeno et al., 1998). The resulting G418-resistant clones are analyzed by FISH and classified into different categories of events.

Transfected cell lines are designated HT-38, HT-47, HT-54, HT-190, and HT-191.

Those skilled in the art will appreciate that the invention described herein is susceptible to variation and modifications other than those specifically described. It is to be understood that the invention includes all such variations and modifications. The invention also includes all of the step, features, compositions and compounds referred to or indicated in this specification, individually or collectively, and any and all combinations of any two or more said steps or features.

TABLE 1

Restriction analysis of the genomic DNA of patient BE and

those of his parents using three overlapping cosmids that

span the marker centromere.

Y7C14

Y6C10

Y4C7

NotI

n.a.

910

910

BssHII

n.a.

815, 340

n.a.

BsiWI

n.a.

740

740

SalI

410

410

410, 540

claI

315, 145, 110, 80

315, 145, 110, 80

315, 145, 110, 80

SnaBI

n.a.

250, 148

n.a.

NaeI

240, 210, 155, 120

240, 210, 155, 120

240, 210, 155, 120

NarI

222, 108, 70

222, 108

222, 200, 108, 70

EclXI

180

180

180

SfiI

170

170

170

KspI

168

168

168

AatII

165, 146

165, 146

165, 146

NheI

38

38

38

BstBI

n.a.

35

35

SmaI

n.a.

90, 40, 22

90, 40, 22

BglI

25

25, 7.2, 6.2

25

PacI

n.a.

25

n.a.

BamHI

24, 19, 15

24, 22*

24, 22*

KpnI

23

23

23, 19

BcII

21

21

21

PstI

9.4, 5.9, 5.1, 4.2, 3.8,

9.4, 3.8, 2.9, 2.7, 2.4,

9.4, 7.1, 4.2, 3.3, 2.9,

3.3, 2.9, 2.4

1.5, 1.1

2.7, 1.9, 1.5, 1.1

XbaI

14

14, 10

10

EaeI

n.a.

15, 12, 8, 6

n.a.

Sphl

16, 7.5

16

16,9

PvulI

14, 7.5

7.5, 6

7.5, 6

HindII

8.6, 6.9, 6.2, 2.7, 1.8,

6.9, 6.2, 5.6, 5.2, 5, 2.7,

6.2, 5.6, 5.2, 4.3, 2.9,

1.2

1.9, 1.8, 1.7, 1.2, 0.6

1.7, 1.2

ApaI

15, 8.5

15

15

11, 4.3, 3.9, 1.9, 1.5

11, 4, 3, 2, 1.9, 1.7, 1.5

10.2, 7.6, 3, 2, 1.9, 1.7,

EcoRI

1.5

HpaII

5.5, 4.3, 3.6, 1.6

6.9, 3.6, 2.8, 1.6, 1.2

3.6, 2.8, 2.5, 1.6, 1.2

MspI

3.9, 3.0, 2.8, 2.5, 2, 1.6

3.9, 3.6, 2.8, 2.5, 2.2,

3.6, 3.2, 2.8, 2.5, 2.2,

1.2

1.6, 1.5, 1.3, 1.2, 0.9

1.6, 1.5. 1.2. 1

SspI

n.a.

10

n.a.

XhoII

7.5

n.a.

n.a.

DraI

7.5

7.5

7.5

BglIl

8.5, 6, 5, 4.7, 3.5, 2.5

6, 5, 4.7, 2.5, 1.6, 1.5, 1

7, 6, 5, 4.7, 2.5, 1.6,

1.5, 1.1, 1

AvaII

7.4, 3.7, 3.4, 2.8, 2.6,

3.7, 2.8, 2.6, 1.8, 1.7,

4.3, 3.7, 2.8, 2.6, 1.8,

1.8, 1.7, 1.4, 1.2, 1.1

1.4, 1.2, 1.1, 0.9, 0.8,

1.7, 1.4, 1.2

0.5

StuI

12.5, 8, 7.5

12.5, 9, 8.5

9, 8.5

HindIII

6.6, 5.4, 4.7, 4.4, 2.9,

5, 4.7, 4.4, 4.1, 2.9, 2.5,

5, 4.7, 4.1, 3.1, 2.5, 2.3,

2.5

0.7

1.9

n.a. = data not available. The values represent restriction fragment lengths in kilobases. Multiple values for an enzyme denote different bands detected by a cosmid probe on a gel lane. Since there were no detectable differences between the DNA of patient BE and those of his parents in any of the fragments (except for a BamHI polymorphic band found in one of the parents, indicated by an asterisk), only one set of values is shown for all three genomic DNA.

TABLE 2

Vectors for cloning centromeric regions from normal

chromosome 10 or mardel (10) DNA into yeast artificial

chromosomes (YACs). These YACs can be shuttled into

mammalian cells to test for function as HACs.

Vector:

Key Feature(s)

Map

pJS97ARTi

hTEL/I-SceI/yTEL, DUFR

FIG. 8A

pJS98ANTi

hTEL/I-SceI/yTEL, neo

FIG. 8B

Fragmentation 1

hTEL/I-SceI/yTEL, hyg

FIG. 8C

Fragmentation 2 (−/+ hGH)

hTEL/I-SceI/yTEL, neo, hGH

FIG. 8D

pVC39-AAH2

ALU-ALU TAR vector

FIG. 8E

pTEL/CAT/TEL

hTEL/I-SceI/hTEL/neo

FIG. 8F

pAAH/TCNa

TAR vector with hTEL/I-SceI/hTEL/neo

FIG. 8G

pVC39-ALU/C3-F2(+/−)

ALU-specifc TAR vectors

FIG. 8H

pTCS

ends of PAC4 in pBS

FIG. 8I

pTCN-TCS

specific TAR vector hTEL/I-SceI/hTEL/neo

FIG. 8J

TABLE 3

PCR analysis of YAC 5f-52-E8 clone and comparison with

the HC-contig/neo-centromere region from normal

chromosome 10 and mar del (10)

Genomic DNA used in PCR (product size in kb)

Primer-Pairs

a

BE2Cl-18-1f

b

BE2Cl-18-5f

b

YAC 5f-52-E8

norm: 141 + 55

1.80

1.80

not present

norm: 32 + 30

0.90

0.90

0.90

norm: 28 + 29

1.00

1.00

1.00

norm: 1 + 3

2.90

2.90

2.90

norm: 39 + 52

1.20

1.20

1.20

norm: 5 + 7

023

0.23

0.23

norm: 16 + 5

3.50

3.50

3.50

norm: 9 + 14

0.90

0.90

0.90

norm. 36 + 37

2.00

2.00

2.00

norm: 168 + 71

4.00

4.00

4.00

norm: 27 + 10

15.90

15.90

15.90

norm: 18 + 17

1.20

1.40

1.40

(VNTR)

c

norm: 68 + 17

8.00

8.00

8.00

norm: 34 + 47

3.00

3.00

3.00

PAC4t7: a + b

0.30

0.30

not present

AFM259xg5: ca + gt

c

0.21

0.19

not present

a

Refer to

FIG. 1a

for the relative positions of each primer-pair.

b

BE2Cl-18-1f and BE2Cl-18-5f are somatic hybrid cell lines containing the normal human chromosome 10 and mar del (10), respectively (2).

c

The ‘norm: 18 + 17’ and ‘AFM259xg5: ca and gt’ primer sets allow distinction between the normal human chromosome 10 and mar del (10) by detecting a VNTR and a microsatellite, respectively.

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29

1

19

DNA

DNA primer

1
ggattacagg yrtgagcca 19

2

19

DNA

DNA primer

2
rccaytgcac tgcagcctg 19

3

80595

DNA

Nucleotide sequence of HC-contig

3
gaattctcct gcctcagcct cccaagtagc tgaggttaca ggtgccagcc accacgtcca 60
gctaattttt gtattttagt agagacgggg tttcaccgtg tttgccaggc tggtatcaaa 120
ctcctgacct caagtgatct gcctgcctca gcctcccaaa atgctaggat tacaggtgtg 180
agtcaccgca cccagccctt ctttcagttc tatcacctct ttttgctata tttgtatgag 240
agctttatta ttaggggcac atacatttaa aattgttatg tcttattgat agattgatct 300
gtcattatga atgtctgtat tcattccctg atagtatttc tttttctaaa tatttttctg 360
aatgtgtctg ctattaacat agccactctg gctttttaaa attagtattt ttatggtata 420
tatttttcct tttttttttt tttaagtttt agatgttatg tttccttata cttaaagtgg 480
gtgtcttata ggcagcatat atctgggtct tgatgtatta tttaatctga taatctcaac 540
ctttttgttg gagtgtttag gccatttaca tttagtgtaa ttatagacat ggtttgattt 600
gctataccat cttttcattt gttttatatg tgagccatct tttcattgtt cttttttcat 660
ctttgaccat tttctttagt actgaatact ttttttgtat ttcattatat ctattggctt 720
tttagttata cctcttaaaa tttttttttc tgttttatgt aggatttata atatacatct 780
ttaacttatc acagattacc ttcaaatagt attttaccag ctcaagtgta atgtagaaac 840
cttacaagag tatattttca tttctgtctc ctaattttta tgctatgtct ataatacatt 900
aggtttgttg ttgtttgttt ttaccttatt gctgttggct ggggtcagca aacattttct 960
gtaaagggct agatagtaca ggcatacctt ggagatactg tgggtttggt tccataccac 1020
cacaataata caaatatgca agaagtggat atcacaataa agtgagtcac acaagtcttt 1080
tggcttccca gtgcatataa aagttttgct tatactacac tgtagtctgt taagtgtgca 1140
atagtgttat gtctaaaaaa acacatacct taattttaaa atgctttatt actaaaaaat 1200
gctaacaatc atttgagcat tcagtgagtt gtaatctttt tgctggtgga aggtcttttc 1260
ttattgatga ctgatcgggg gtcaggtgct gaagcttagg gtggctgtgg cagtttctta 1320
aaacaacagt gaagattgca atatcagttg actcttcctt tcatgaaaga tttctctcta 1380
gtgtgtgatg ctttttgata gcattttatg cacagtagaa cttctttgaa aattggatca 1440
atcctctcaa accctgctct gctttaacaa cctaagttaa tataatattc tgaatccatt 1500
gttgtcattt caacaatttt cacagtgtct tcaccaggag tagattccat ctcatttcct 1560
gagatggaat ctttgctcat ccataagaag aaattcctca tctgttcaag ttttatcatg 1620
agattgcagc aatacagtca tgtcttcagg cctcacttca cttttaattc cagttctctt 1680
gctgtttcta ccacatctgt ggttccttcc tccattgaag tcttgaacct ctccaagtca 1740
tccatgaggg ttggaatcga cttcttccaa attcctgtta atatttatat tttgacctcc 1800
catgaatcat gaatgttctt aatggcacct ggaatggtga atcctttcca aaaggttttc 1860
aatttactta gtccagatcc atccatccag aggatccact ttcaatgcca gttatagcct 1920
tatggaatgt atttcttcaa taataaggct tgaaagttga aattactcct tgatccattt 1980
tctgcaaaat agatgttgtg ttagcaggca tgaaagcaac attaatcttt ttgtacatgt 2040
ccatcagagc tcttgggtga ccaggtatat tgccagtgag cagtaatact ttgaaaggaa 2100
ttatttttct tagcagtagg tctcaacaat gggcttaaaa tatttggtcc accattctgt 2160
aaactgatgt gctgtcatct aaactttgta gtttcattta tagagcacag gcagagtaga 2220
tgtagcataa ttcttaaggg acttaggatt ttcagaatgg taaatgaaca ttggcatcaa 2280
tttaaatcac tagctgtatt agcccccaac aagagagtca gcctattttt tgaagctttg 2340
aagccaagcg tcgacttctc ctccctggtt acaaaagtcc taaatggcat cttcttccaa 2400
tataaggctg ttttatctac attgaaaatc tgttgtttag tgtagccacc ttcatcaatg 2460
atactatcta gatctcttgg ataacttgtg cagcttctac atcagcattt gctacttcac 2520
cttgtactct tatgtaatgg agtggcatct ttcctcgtac ctcatgaacc aacctctgct 2580
agcttccaac ttttcttctg tagtttcctc gcctctctca gccttcatag acttgaggat 2640
agttagagac ttgctttgga ttagattttg gcttcaggaa atgttgtggc tggtttgatc 2700
ttctatccag accactaaaa ctttatccat atcagcaata aggctgtttt gctttcttat 2760
tatttgtgtg ttcactggag tagcactttt aatttgcttc aagatatatt tctttgcatt 2820
cacaacttgg ctgactggtg caagaggcct agctttcaga ctatcttggc ttttgacatg 2880
ccttcctcac taagcttaat catttctagc ttttgattta aaatgagaga tgtaggccag 2940
gcacagtggc aggcacagtg gcatatgcct gtaattccaa cacattaaga ggccaaggtg 3000
ggaggattgc ttgaacccag gaggtggagg ttgtagagat cacaccactg cattccgtcc 3060
tggatgacag agcaagacct ttctcaaaat aaaatgagag gtgtgcttct tctttttgtt 3120
tgagcccata gaagccatag tatgattttt aattggccta atttcaatac tgttgtgtct 3180
cagagaatag ggaggtctga agagagggag agaggtgggg gaatggctgg tcagtggagc 3240
agtcagaaca cacataacac taataaattg tttgctgtct tatatggatg tggtttgtga 3300
tgcccccaaa caattacaat agttacagca aatatcactg atcacagatc accataacag 3360
atataagaat catggcaaag tttgaaatat tcttgagaat tagcaaagtg tgacacagag 3420
aaacaaagtg agcacatgcc gttggaaaaa attggtgttg atagacttgc tccatcgcaa 3480
gtttgccata cgccttcaat ttataaaaaa cacaatatct aggaagttca ataaagtgaa 3540
gtgcaataag atgaagtatg cctgtaaata tttcaggctt tccagaccat agggtttctg 3600
ttgcaactgc tcacctctgc cattatagca tgaaagcagc tatagaaaat atacataaat 3660
gaggcctgta atcccaacac tttgggagcc caaggtggat ggatcacttg aggtcaggaa 3720
ttcgagacca gcttggccaa catggcaaaa ccccgtctct actaaaaata caaaaatgag 3780
ccaggactac gcatgcctgt agtcccagct acttgggagg ctgaggcagg agaatctctt 3840
gaacccggga aggggaggtt acagtgagcc aagattgtgc cactgcactc cagcctgggc 3900
aacagagtga gactgtctca caaaaaaaaa aaaaggaaaa gaaaatacac ataaatgaat 3960
gtatgtggct gtgtaccagt atatcctcat gctctagctt gccaaccctt gctttacact 4020
gtcagttacc ttctaaagag attaaaaatc ataacaatat ctattacgtt tattcacatc 4080
ctagtgtcat ttcttcctta tgtagaatca aatttcattc tggtatcata tttcttcttt 4140
ctaaataatt tcctttaata ttttttatag cacaggtcta atagcaatgc attatgcaat 4200
tcattgctat tagacctgtg ctataaaata gcaatgaatt atgtcagttt ttatttgtct 4260
gaaaaagttt tttgtttttg aaatatactt ttgctgggta tataaatcca tgttgcataa 4320
cttctctttt cttcagcact ttaatgaagt cactcagtta tcttctggct tgtatagttt 4380
ctctggctgc cttcaagatt ttttcattgt ctttaatttt tagcagtttg atgtgtctag 4440
gagtgatttt ctttgtattt atccttttgg gggcctctta atttctttga tccttttttt 4500
cttttttttt tttttttaat cagttttggt ctgtctcctc aagtgggctg aaaaaaaaag 4560
aaaaataaaa tcatagttta aaaaactaat tttggaaaat tttcagctat catttcttca 4620
aatatttatc ctactctatg ctcccctcct cccctttcct tctgtgactc aaattacagg 4680
tatatttaac cattttattt gttcacggca cttggatgct ctgctttctt attttttgtc 4740
tttcattttg gataatttct actgacctat cttcaagttc actgattctt ttctcagtca 4800
tatctagtgt gctcaacgcc tgttgaagaa atcctttgtc tttaatatca tgttttttat 4860
ttctagcatt ttcatgtaac tctttgttct ggtttccatc tctctactca cttttttttt 4920
tttttttttt tttttttgag acagagtctc gctctgtcac ccaggctgga gtgtagtggc 4980
gcgatctcgg ctcactgcaa cttccgtccc ctgggttcaa gtgattctcc tgcctcatcc 5040
tcccgagtag ttggaattac aggtgcccac caccgtggct ggctaatttt tgtatttttt 5100
tagtggaaac agggtttcac catgttggcc aggctggtct tgaattcctg acctcaggtg 5160
atccacctgc ctcagcctcc ccaattgctg aaattactgg catgaggcac tgcacccagc 5220
tctgctgaca ttttttatct tttgctgcat tttgtctacc ttttccatga aatcctttaa 5280
catagtagtc atagttactt tcaattcctt gtctgacagt tctgacattc aagtctaggt 5340
ctgttaatag ctttgtgagt ctgttaacag ctttttttca ttcttgtctg tgtgttttgt 5400
atttcttgat tgtatgccaa atattgcctg taaaataaac ttagataagt catacttcta 5460
tccagaaata ggcacatttt ttgtgtccag tcattagtgt ggagggaggt tggggcagtc 5520
tagtcagtgg ctgaactagg tttggatttg ttgatgctat acttagaatg caccagactt 5580
ccattcactg caagagtggg ctgctgcgct ttgtgattca tgtgaggcct gaattgtggg 5640
tttttcctta gtgtgtccct ccatgctcag atttcagcaa gtcttcatat ctgtgccaca 5700
gaaggaatct gacccatgct ctttttgacc tccccaagtg atcaactgtt gcttgttata 5760
gcttgtcatg gagtaagagg gtgttttttt agttttcatc ctccagcctt ggtcttgggc 5820
cctgagctcc tagactccag gagtggatgg aatccagtga tttctcagta attcagcccc 5880
ttctccagta gtggcagatc tctgctttgt atcagtgcaa gatcctgggc tgagctcatt 5940
ttctgccctt cctcgagtgg cagacagctc ttgctttcac ccttctacca aaggcagtgc 6000
atcttttctt gggcctctcc ccattgaact tatgactttc acataagaga agggctcatg 6060
tatcagagaa ttctgtgact ttgtgccaca tacagagtct ctcagttctc ttgccctgcc 6120
ccagtctttt ttgtgagcac ctagtagaga cccttggaga agagcaagga agcgagtatg 6180
gacttctttt gtgtctgtcg attgctttgt ttctcaactg ctactcttgg actttaagaa 6240
ttcattaaaa tttcagctgt tttcttttat tctttttgtt tttctttttt tttttttttt 6300
tttttagatg gagtcttgct ctgttgccca ggctggagtg cagtggtgtg atcttggctt 6360
gctgcaacct ccgcctcccg ggttcaagcg attctcctgc ctcagcctcc caagtagttg 6420
ggattacagg tgcccaccac cacacctggc taatttttgt atttttagta gacacagggt 6480
ttcaccattt tggtcaggct tgtctcaaac tcctgacctc atgatctgcc cgcctcagcc 6540
tcccaaagtg ctgggattac aggcatgagc caccgcgcca ggcctcagct gttctctttt 6600
tacctgctgg gatggctagt tttctgtgtc aacttgactg ggccatggga tgtccagata 6660
tgtaattaaa cagtatttct gggtgtttct gtgagggtgt cttcagaaga gatttgcatt 6720
tgaattggtg aactaagtaa agcagagggc cctgtctagt aggggtaggc atcatccagt 6780
ctgttgagga cttgaataga acaaaaggca ggggaaggtt ggaattgccc cctctctgct 6840
tgagctgaga catctatcct gcccttggca ctcctggttc tcaggggttc agacctggat 6900
tcctggtctc caccttgccc atggcagact gtgggacttc tcagcctcct atctaattaa 6960
taaatctctt catacacaca cacacacaca cacacacaca cacacacaca cacacacaca 7020
ccctatgtat ccttctgttt ctctgcagaa ccatatctaa tacacctgct tttatgacga 7080
ttacctatcg attctgtatt ctgccaaaac tgaaaacagt tcatttttcc atctcttctc 7140
agagaggctt gtcagccatt agttctctga tgggctcaag aagttatgca gttttttttt 7200
tctcactgtt aggatggaat tgatattctg ttgaaacttt ctatacctaa gtggaaactt 7260
gttttgaggt tattttctct acttactttt gctggaaatg gaacactctg tatctagtta 7320
agacacataa actgacttgt gataccataa tgttgtgttg aattttatat tcttagaaaa 7380
tcatctgtca aggtgttaac taatggcaaa gcatttaata aatcagcatt catgtattca 7440
ggtgctctga attatctgac ttttaaattc ttactttata aatgagaaaa ttggggcatg 7500
gaaaagttaa ctctcctaac cccgaattat tacattatta aggacaggac ttagaggcca 7560
gatatcttaa gtcattaata ttctttggct cacagaattg gcagtataac ctaaaggtaa 7620
taactaggtg attttctttt atatcaatta aatatgtcag ttttcaaata ttcataagta 7680
cctactgtgc agggaaagaa catgccatac aaaagatgta gtccaggcct ttaagaaact 7740
ttcatttaat gggaactcaa gaagtgtaca tataaggagg gaagtagcag tatggtacaa 7800
gataatacat acatatcagt gaatgatatt gccaaaaagt gctattgata gagaaataat 7860
tcatttctgc aaacagctgc tgatctccta ctgaaaacag aggagggaga acaggacgcc 7920
tcgtggtcag gatagaagag aaagaccttg agttgagcct tgaacagtat ttaatattca 7980
aaaggttaag agaggagagc aattgaggag gggagaatag ttccagcaca aatgatggtg 8040
tacaagatga acacagtcag taaagagcag actggtctgg atggagagga ggatttgcat 8100
catttgggat tacgtcattt agacccttga aagccaggat tgagtaaagc cacagtgaag 8160
cgactggctc gtatggaagc tttattttaa gaagattaat ctggtagtga catgtgccaa 8220
aaactgaata ggtagaaatg agatgcagag agcccagtta gaactaagtc tggtgcagta 8280
atgcaggatt gaggcaataa acaccaaact acagtatcac cagataatgg atgtttgaac 8340
ggacggttta aaggaaaatt gatggtattt ggtaatttat tagataatcc agggccatgg 8400
aatgagaggg gaaaatgact aaccatagtc atcaaatggt ttttcttaat gaatctgaat 8460
tttggtgtaa gagcaacatt ttcttaggcc ttgcctagtt ggtacagctg actatgataa 8520
tgactgctac catgcttgtt cctcttttag cagctgtgag tcccccacca gccaaacaat 8580
gagcctcttg aaaaggacga tgccttttca cttctctcca agtgcttggc aaataggagg 8640
ccttttgaag ttactttata gttaggggtt cccagtgagt atttgaaata ttaagtcatg 8700
cccgtggttg acagcatggc cctactgctc atcatcagct attaacctta ggcaagttaa 8760
tgaacttttc taagccccag tctactcatt tataaagtgg gattattaat aatgtctact 8820
tcataaaatt atgaagcctg agttaggtca ttcagatagt gtttagtctg attcttcgaa 8880
cctagtaaac agtcagtaaa cagaagcaaa tgccacatgc ctgatttata tccaagggga 8940
gaaaggtaaa agtgaaattt tcatgattta tggattcaaa ttatacattt caaagatgct 9000
ttataagcta ttgttttggt aagaagaatt gagctgaaac agaattttct gacagcagtg 9060
attattaaat ggtgaaatag gctattgatg tctttagagg atatagatgt tcaccttttg 9120
catataagtg cacaaaaatt cactaagtag atatgtctgt ctacacagag agagagagcg 9180
tgagagcatt aaagttagta aacatccccc tcgctttttt ttttttgaga cagggtctta 9240
ctctgttgcc taggctggag tgcagtggtg caatcgtggc tcactgcagt ctcaacatcc 9300
tgggctcaag cgatcctctc gctcagcctc ctgagtagct gaggtgtgca ccaccacacc 9360
cggctaattt ttaaattttt ttattgtaaa ggtgaggttt caccatgttg cccaggtctc 9420
aaactcctga gctcaagcaa tctgctcact tcagcctcca aaaatgctgg gattacaggc 9480
gtgagccacc acgcctggcc agtaaacccc attcatttac atcatcttac ttgtccctcc 9540
aaaatcctgc aaagtaggta ggttctgtct ttatttgtta tttaggtgaa gaacttgaag 9600
tggtgttgag gaataggtgt tttgccaaga gtcacgcagc tggagtggca gagctgtata 9660
ctcttctgat tccaccaacg ctgtttacat cacatctgga gaaaagtgct ctgaggcaca 9720
gatgtttagt gggagggatg agacacaggc tgcaatgcct aaagataatc gggaataaaa 9780
gcagaaaaca agacgtttgt ttctgttaaa atgagacaga aaataaggcg tttgttgttt 9840
gggattgagc acttggagaa gtggggagcg atttgatttg ggtgagactg ctcctggaat 9900
gctgcatctg gttctggact actcattact aggcttatag aaactagctg gaggaggttc 9960
aaagaaaagc tccaaaatga ttagcgggct gacgggattg atttataaga aatattaaaa 10020
gaattaaatg tgtatagctc agctaagcaa agatgaaaga gaccagctaa atgtatacaa 10080
atatctgaaa cgtgcaaact ttaaaaagag agattaatta tttaacatga tacacggggg 10140
cacaatatgc agtcacagga tgaaaatttc agctgagtat ctagaagaat tccccgatag 10200
tgaatctgtt aaggctgtct gtagtgtggc ctttccctgg agaggcaata gaaatttcaa 10260
gtcttacgat tttaaaagtt tcttgggaac taggtattag atgatgttag agaattatta 10320
ttaatttggt caggtatgat aatggtattg tagttctata agaaaaattg tattttttag 10380
agttacatac cctgaaatat aagcatagaa tatgatgtag gagatttgct ttaaaatacc 10440
acagtaagga aagaaaggaa ggaggaagaa aagaaaggaa ggggaagaaa gggaaaaaga 10500
ggcaaagaag gaagagaagg taagagaaag aaaaagaatg aaggaagaag gctgggcact 10560
gtggctcatg cctataatcc cagcatttag gaggccaagt tgggaggatc acttaattaa 10620
gcccaggagt tcaaggctgc agtgagctgt gattgcgcca ctgcactcca gcctgggtgg 10680
cagagtgaag ccctgtctct aaaaaaaaaa aataagttaa aaagaaagaa aaggatagat 10740
gaagtatggc aagatgttgg taatgttgaa cctgaaggaa gttaatatgt gagttcactt 10800
tcctcttcag tcttctttat gtatgtttgc caactttcat aataaacaat ttaaattata 10860
ttttcctgat caaaacttag tagcagtatt aatccctggg cttcctgact agaacagcct 10920
cattaccaca tgggcagagt tctggccgac cagggaccac gtagtggttc accatcttgc 10980
tctggtaatg tggtctgggc tgaagggccc tttctaaggt tgtagataga aatccaggaa 11040
acttgttaga actgcagacc tatcagggta cctgcaggag gtgagtctac taaggtgaaa 11100
aagcagaggg cagaggtcgt gattagcagc tgaccgcccc ctgcttttct gtccctcatt 11160
cgtggaaaat tgagtggagc tcaattttga gtggagctct aagtagctcc acttgtagac 11220
attgagtgga gctctaagtg tcttcagaat agcaaaacac tagttttctt tttcttttct 11280
tttttttttt ttttggagac agagtcttgg tctgtcgccc aggctggagt gcaatggcac 11340
gatctccgct cactgaactc tgcctcccgg gttcaagcga ctctcctgcc tcagcctccc 11400
gagtagctgg gattacaggt gcccaccacc acgcccagct aattttccta tttttagtag 11460
agatgaggtt tcaccgtgtt ggccaggctg gtctcaaact cctggcctca agtgatccgc 11520
ctgccttggc ctcccaaagt cctgggatta caggtgtgag ccaccacacc cagctgcaaa 11580
accctatttt tcttgaatgg agaaacactt tccccttatt tattgagttt gggaagcaag 11640
aagaggggta attcattaag tgaaaatttc caaaatccag aaaacatcga taaagcagca 11700
gcttaatttt tttaaggaag aattttttaa actatcttct tttgagcctc tttaggaaga 11760
cctcacgtcc ttgccttgaa tgttgagagt gggaaatcca gggagttttg gaatgcatgc 11820
cttatgtctg cttttttgtt tgttagagaa atataaatat tttatctagg ttttgctgat 11880
ggcagtcaag catgaacaca acccactgtt tgagaagctg taatttctga atttctgcag 11940
agtgcacatc taggccagca aatggcagta agagtgaggt ggatttagct cagtgtaagg 12000
atgaactcca gaaccatcgg ctctgactga aagtgaagcg gcagccgcgt tgtgggaaag 12060
ctggctggag tctctctcat aagcaggcat tctttttctc cagcccgtca ctgtgttggt 12120
ttgggcccac ggtaagcctc ctggcctcta ggctgtaacc cccaccatcc tcctctgcct 12180
cgcctccaga gtgattgttc tgaagcacaa ctggatgtca ttccccttcc tgaactccta 12240
gcacctacag ggactccatc ccttgtgccc cacatacctc acacgtagac attcctaatg 12300
aagatttgat tgaattattg taaactcagt gcctcccact cttctagttg cctctctgcc 12360
tgcctttgta catttattta tttatttatt tatttattta tttatgagac agagtcttac 12420
tgtatcaccc aggctggagt ttagtggcac catctcagct cactgcaact tacctcccag 12480
atcaagcaat cctcccacct cagcctcccg aggagctggg accataggca cgtgccatat 12540
gcccggttaa tttattgtaa tttttgtaga gatggggttt catcgtgttg cccaggctag 12600
tcttgaactc ctggactcag gcgattcgcc cgtctcagtc tcccaaagtg ctgggattat 12660
aggcgtgagc caccatgccc agccgctagc actcatctta atcgtatatt tacttatctg 12720
gctttcccac cagactgcgg gctcttcaag agtaaatgcc atgttttcac ctttatttcc 12780
ccagtttgtg gcacattcta ggcactcgcc atcatgaaat aaacctctgg agctgtgata 12840
ttacaaacgt ggaaagatga cgagcactca gcaactttca gtgagtaaac aaaggctttc 12900
attcagcatg atttattgac tgcccaaatc tgggctgctt cctgtctgtg gttcaaggag 12960
agcatagtct acagaaccag agacctggct actctggaag ttagacttaa gcccaccccg 13020
gtccttgaat ggggaaatat ttcccttcat tcctgtgttt tagggacaga aagatgagta 13080
atgcagtgat acatgctgga aatgtttatt ccactacccg aagctgcctc tcaacttaac 13140
aatccatgaa agaaacaaga tggtatataa ctttttctaa tttgtgatgc ctttgtttat 13200
ttgtttccgg ttaaaagagg aggtggcatt gaattgtttg tttggtttgg tttcttcttc 13260
aataagaagc atcttaatat aactagactg gacatctgtc ccattttcaa aaattacaag 13320
tttcgatcat tgctaaattg tacagatccc aatctgtctg ctctgcatac atttgcattt 13380
ataaaagcag aagcagacta gcagtctttc taatgcaatc ccccaaatgc atgaagtatt 13440
agattgcttc tccctattgg ttcatgcatt gctaaaggct taaaaggatc attgatttta 13500
attatttaat gtgtacagca ggctgagctt cctttctttt ttaagggaag aaccttcagg 13560
ggcattgctt tagtttttta atgttaaatc tcatttttct ttgaaaataa gaagttaaag 13620
ctgtattcac acaagctctc aaagtgccag attttcattg tgtttttaaa ccatctagga 13680
aatgtttgat tctaatgaaa cattactgct gaaaattggg ctgaaattgc tgggctggaa 13740
atattgttat aacttcacat gattccagtg ttgtattatt attttttctt tttctttttt 13800
tgacccgata tagatgaagc gaagagacaa ggagcaatcc catgtgtaat agaaaaaggc 13860
agcctgaatt gttgttgctg tttttgaaat ttaagctggt tttcgattaa attcagtaaa 13920
tggtccagga ctataaatgt tgaacatttt ttaccgtgtg atttaaattt tagtcttatt 13980
gttttttttt tttttgatgg tttacatttt ccccatggga agcagctatg tcatgtcggc 14040
atgattcatc atggtaacat ctcgggttat tttggtttgt gttatgttca gaaagcggaa 14100
tgccaaaaat aaagagtggt ttgtgatgtc tagtgtgtct tcctttaaca aatcaaaggc 14160
ttttatttaa tccacttaat gggacactgc agaaatttaa aaaatggaag tcccatccac 14220
agaaggcagg tactatgatg taaaaagttt aggtggggga ttaatagagt gatcatataa 14280
tttatgagct aaaccggagg cacttttttt tttgagatcg agtctcactg ttgcctaggc 14340
tggagtgcag tgacgtgatc acagctcact gcaacctccg cctcccgggt tcaagcgatt 14400
ctcatgcctc agcctcctga gtagctggga ctataggcgc ccaccaccat gcccagctaa 14460
tttttgtgtt ttttgtagag atggggtttc accatgttgg ccaggcttgt ctcaaactcc 14520
tgacctcagg tgatccgccc acctcgacct cctaaactgc tgggattaca ggcgtaagcc 14580
accatgcctg gcccagagac acttttgaga gtgaagagga agctgagaat aattcactga 14640
tctacaactg ggaccatcca gggcaagcca gatgccatta ccactagcta gaaagcttgc 14700
caaggtctca tttaccttgg tatatagcaa attcttcttt tgaattctgg aaattctggt 14760
aagtcattga ggtagctctg tgccaaggag caatatggta gaattctaat atttcaggca 14820
gacaacactt tcctgcattt gtagcaggta aagggaggtc agggcagaag acaaaaccac 14880
tgggactcga caaagggcat aaacgtctaa tgcacctgat gtagctgatg gtaaattgtt 14940
atcagctaaa gatctttcat aataaataaa cttatcattt gtaggagggc acagaaatcg 15000
tggaaagctg ggattcaggt tgcctgtggc tttaattctg gaatcagaaa tattagtcaa 15060
ggatatcagt ctatgaagta agttttcaat gttatatgcc acaagatgca gctgtcctat 15120
tttcacttcc agtaattcct tctgaattaa tacaccttaa aaatagctgc agcttctcaa 15180
atctgtgaga atcgtatgtg ctgcttgcta cactttcttt ttcctgaagg ctctttgagg 15240
tctttcaaga actcaattca attcagcaac aattaggggg tctaaggtat acagacgctg 15300
tgcaagatgc tcctgagaca caaagaggag gtcaagcccc tgccttcagg cacctctcta 15360
taatatagga ggagaaagag aagaaacact aatacacata ggtaggtgcc attaaaaggg 15420
tacatacatt aaagccaggt ggtaggtgta agaagatttg taacatgaga attttctgca 15480
tgtttgaaat atcttataat ttttaaaaat taaaatggga gatacatata tatgtattta 15540
tgtatgtata tatgtatgta catatacaca catatataca taaatatata cataaatatg 15600
tatatatgtg tatatagaca taaatatgta tatatgtgta tatatacata aatatgtata 15660
tatgtgtata tagacataaa tatgtatata tgtgtatata gacataaata tgtatatatg 15720
tgtatataga cataaatatg tatatatgtg tatatagaca taaatatgta tatatgtgta 15780
tatagacata aatatgtata tatgtgtata tagacataaa tatgtatata tgtgtatata 15840
gacataaata tgtatatatg tgtatataga cataaatatg tatatatgtg tatatagaca 15900
taaatatgta tatatgtgta tatagacata aatatgtata tatgtgtata tagacataaa 15960
tatgtatata tgtgtatata gacataaata tgtatatatg tgtatataga cataaatatg 16020
tatatatgtt gtatatagac ataaatatgt atatatgtgt gtatataata atgtgtgtca 16080
tatacacaca tatatacata cataaacatt ctgcattata ccattcactt tgtaacccat 16140
cttccctaaa aactgtctca taaagagtct tcttttccct gtacctatgc aatggtaagt 16200
agcaaaacac acattctttt gggtccccat aacattccct gtagtttgcc cttaacagtc 16260
tttgatgtga aatttactgt ttctgtctta accttgcctg tctcgcgtac atggagtttt 16320
ggctcctggc tcctagtctg catcttcacc ccatcccttg cccaaagaat ctggttatgt 16380
gaccactgct catcttttct gctgccacaa ctccagtcca agccacaaac ctctctctcc 16440
tggactcctg cggggagttc ctttctctcc ctgcatgagt ctattctccg cacaactggc 16500
ataggtaagt gagactgcgg aagaggcaag tttgcaagtc cagaggaaat gaagactctg 16560
cttgtgcaca tgctgggttt gacgggtgct ggatatccga tggatggccc ttaaggtgag 16620
ctcaaggctt aagggagaga taggggctga tgatctgaga ttcatcagtg tgtggctgat 16680
gtttaaaccc aggggacagg ataagaaggt tattccaggg agagcgtaga taaagaagct 16740
aaatggcttc tgggtcctta gtcattcaaa atcggacctc tgaggcagga ggaaagccca 16800
gaaagagtag attcctggga ctcacgggat aaagactttc aaaaagtggg ggctggccag 16860
tgctgctgaa ggaagtagca ggaccggaac agaagggtaa tcgttggacc tggagaactt 16920
gaatttgaat tttaaggttg gtaaccttaa aaaagagcaa ttttagatac cttttgaaat 16980
tatttgcaag atttgtttgg tatatgtgtt attccaggca aagggaccag aaaagtaaaa 17040
aatacttact gaacagttac tgcatgcctg gcactgtaac accctgttta attctcacgg 17100
caaccctata gagtaggtgt catcatcccc atcttacaga tgaggatatg aggtgcagct 17160
agattaagca gtttgcctca ggttacacca actggttaac gtagagctag gatttgaacc 17220
cggatgggct gatcccagag ctcatgcttt aaatcgctag actggtgctc acagaagact 17280
gggaccgaaa aaaattaata aaaaaaataa ggagccccct gggctagcaa attaggagtt 17340
gttcagacag atgtgaaaag gaaagcaagg cagagggaaa gtcactgtac agaagagaga 17400
gacccatgac agcagagaca gtgagctggt aaagtggctg gcgatctagc ccctgaaaat 17460
acctccagag aggcaggctc acgcctgtaa tcccagcact ttgggaggcc gaggtgggca 17520
gatcacctga ggtcaggagt ttgagaccag cctggccaat ggcgaaatcc cgtctctact 17580
aaaaatacaa aaattagccg agcatggtga caggcacctg taatcccagc tgttcagttg 17640
gctgagtcag gagaatagcc tggatccggg aagtggaggt tgtagtaagc caagattgcg 17700
ccactgcatg ccagcctggg cgacagagca agacttttct taaaacaaac aaacaaaaaa 17760
gaaaaaagaa aaggaaagaa gaaagagaca aagaaagaaa gagagaagga aagaaaggaa 17820
ggaaggaaga gaaggaagga aggaaagaaa gaaaaggaaa gaaagaaaaa gaaagaagaa 17880
agaaaggaaa gaaaagaaag aaaaagaaag aaagaaaata cctccagaga gccaggtctc 17940
ttaggccttc tgagaaactc acatcccttt tgatgaacac aaatgcttca cactctcaat 18000
gttattggta atccaagtta tcaatatacc taaatcactt agtactgaat ctggcatata 18060
gtaatcacct aatgaagaga taagagtcat ggagtattct gaagcaatta gaatcaatag 18120
actcaatata cacatggcaa caaagttgga tcttaaaaac cgacctgagt gaaaaaggaa 18180
agggaaagat acataacacg gtaccattat gtaaattgat aatatatgct tacacaattt 18240
gtaagaacac atacaaatag atacatgtat attaaatata ctcgaacggt tacctatggg 18300
gtggtggctg gagtgggggt aagtccgtaa gctgtaatgg aacctaaaca aatacatgaa 18360
acgagtagga atcagaagga gtaacaataa aaatgtgcca tgaactgagg agtgtaaatt 18420
aatcaactca ctgcatctga ggttaaaaat agaaagatga taattgttat tcttattact 18480
cgtaggtctt ccacttgcac tcagctttac aatgttggac tatccttcag atggcaccct 18540
ccttgcactt gctcaggcag gagagctttt tcctccagct ttctaggtga tttaatatat 18600
cagggaataa gtataaaaaa aggcacggtg ctccctgggt agcctttctg gacttcagag 18660
ctaaattgca aagtcagttt tacacatgtg atttcatcta tgaaattagg gcaaggtaga 18720
aaactggcac agaaaaaatg tgatttatta tggtgttact atcccttaca agcggagtgt 18780
cagctgcctc tttttgtcca ctgatttaag gcaagatgaa ctgaaagtgg ctatgatcac 18840
gtcttcaaaa gcacactctg gcccctcggc tgcaggcgcc ctgcacattc cccagctgcg 18900
tgtccggtgg tgacacagtg cataattgtg gcgccttcct ggtgcaaact gtctcactta 18960
gctccgtctt gctggcacag cagaaaggaa gaaatcgaaa atgtttggat ttcaaaggta 19020
acaagaagct ggaaaacaac tactggccga gtctgagagt ttcagcggag actggtgcag 19080
ccttgtgttt ttccactgac agctgaaaat gagcccagct tcagtgaagc ttgtttcctt 19140
ccctcctcaa ggttacccac aattctcagt tctctcagga aagccaaaaa atgaatttga 19200
gggtttagga ttgtggttct tttatctatt acaggattga taatatgttc ctccaccaga 19260
tgttctgctt gtaacaatac tcacttcctg acactactgc atatgcagga gtgttactac 19320
caaggtaaac acagaattgg ctgcccaatt ccaaatccct gaactgagtg agagaaatca 19380
gaattataat aggggattca acagagctgg ctacggatgt gccagtggtc agatactttg 19440
ctcatcatac gcaggtgctg ctgctctagc aactgctcac tgcttcattt cctgccttgg 19500
tctttaaata ctgcttttct cagctcaatt ggctttcttc cctctggcag tcacgtttct 19560
ttgggtcaaa cagcaaatga ttctttagaa tcacctggta ctcaaaggag ctacaagaca 19620
ttgggcatcc acttccactc tcttggaaaa acaattttat ggaagccaag gttgccatag 19680
tgcctcttga ggttgtttgc tcagccaagg cccaagcttt gtgcttcaaa catgaaatta 19740
gagagcttca gaacaagatc cacattttca atggcctcac ccaactggat aaaagaacaa 19800
ttgccatatc tcaatgacca cctttttcag gtgggatggt agatgctgga atgggtcaca 19860
gcattgccca accaaacttt gcaaaaaagg ctggaagctc tgactgggga ccctaaatat 19920
gcaaaagttg ataggctctt catgcagaat atgaaccccg tgtatggata tagctaaagg 19980
gttggccttt atgtttctat tccttcacaa acctggtaga atagatatgc ttgtttccct 20040
ttaaaaaatg tcaacaattg catttatgat gctgtgtata gtaactcaca gatcatgctc 20100
catgaaaatg cttcagaacc caatataagg agatttttta gccatgtgtg acaaaagaga 20160
ggccatttca gtgttgaaat tgttcagaga agtatttgat tatgttttct cagatctttt 20220
tatttttatt ttttttgaaa cagagtctca ctttgtcacc caggctggag tacagtggct 20280
gtggtctcgg ctcactgcaa cctctgcctc ccaggttcaa gcgattctcc tgtcagcttc 20340
ccgaatagct gggattacag gcgcatgcac caccatgcct aatttttgta tttttagtag 20400
agacagagtt tcgccatgtt gaccaggctt gccttgaact cctgacttca ggtgatccac 20460
ccacctcagc ctcccaaagc actgggatta caggcatgag ccaccgtgcc cagcctgttt 20520
tctcagatcc tgtatttgtt tctgaagcct tcatttctat cttcttattc attttggaag 20580
tagtacacct aagtaaggtt tttaacaatc aaatatcttt ggaaaattcc ctggttcctt 20640
tcttattcct acaaaaatat gttcagtata gctgatgtta tgtttctttc aaattattca 20700
tttctctatc tcagaattta tctcatgcct aattgttatt gaatagtctt cacttcttgt 20760
catccagttt ctggtctctt atttcactct aagtctaagt ggctattaga ataaagagct 20820
tgtaacagat tctttctcca atatgtctta tcttttgact gcatgccagt gacaaactgt 20880
taactgtttt gattcttcat aacattccac agaacatgct gactcctctc ttcctgaaag 20940
caatgcccaa gcacagcatt gttagatagt atgtacgcaa cagggacatg ggtgcatagc 21000
aaaaactaga aggaaggagg accttcctta gcaatgggtg atatggtccc tggacttaga 21060
ctccaaaggg tcgtgaggtg aaacacacat cgtccatacc caggaagcac acaggtggga 21120
tggaagagct gtgcctaatg aaacttcatc cacgtggagg tggaggaggc tgcagctgca 21180
agaactcaga gctgccttac ccagaccagg gaccagggag ggctttctgg aggaaacagc 21240
ctctgaactg ccagctgata gaggagctct acctcaactc ttctggttcc ccagggctgc 21300
ttttccacgt ccatttattg gcactgaagt ttgaatacct tcaggggccc gaaagcctgc 21360
caggtcctct tctctgcaga gcaatcacac caacctgcaa agggctagga aagggctgtc 21420
atcatctcct actcagaaac tggttcactg gaaggactca ggggccactg aatacatcct 21480
ggcagctttc acaagaaggg cttctgactc aaggatgttt ccatctttgc caggtcgcct 21540
tttctccttc tcttagagtt tggaggacgc aaatgtgctg agaagtcaac ctttcctgca 21600
aggtgagaca caagggcctt tcccagcaga aagaagagag caaatggaag gtccttcttc 21660
ctccagtaga ggatggactc tgtctggcag ccacccaaca ggaaaagcac aatgcatgcc 21720
tgcctgcttc cctccctccc tccgtttctc cctccctccc tccttcctcc cttccattct 21780
cttcccttcc cctcccttcc cttcccctcc cttcccttcc cctcccctcc ccttcccttc 21840
tccctctcct tcccttcctc ttcccttcct tcctcttccc ttcctttccc ctccccttcc 21900
tttcccttcc tccctccctt cctcccttct ttccttccct tctttccttc ctcatttcct 21960
cccttccttc cttccttcct tcctttcttc ctactttcct acctttaggg ctctgtgtct 22020
ttggagtcca ttctgattat gctgtaatgt ctgccccttc ctcttctctg tcaaaaaatg 22080
aaagacatgg aagccacttg ccttttactg aattaaaaat tagtaaaaga gctaaaaatt 22140
aatggttaaa aatgtacgca taaattatgc agtatactaa ccaatgaaaa gatacacttc 22200
tcttaattaa aagctgacag ggagggaaac aagaaaagag aaacacaaaa caataatcta 22260
aatgacctat tagttggaag aacaacatca gagaaaatag atactgtgta tagtcatgtg 22320
tatgtctatg gaataacatt tgtagagaaa tctggactga tcctttctga gtaaagagag 22380
ctgtgggtac aattaagggg agattgaaag gaatccaaaa gcatagcaga tgctgtgcct 22440
cactggaatg gttgccgatc tcctccaaac tatgaagtgt ttgaggctca actttaatat 22500
aattaagata caaagacaga atgagagaaa gagagaaggg agctcactgg aagaacactc 22560
aagattcctt actactcatt ctctaaaatt acaattgttc tagatggaaa agaaaaaaag 22620
cttctctgtt aaaaaaggag cttgtgctat aggaggttta aaatatactt ctgacccatc 22680
tccaacattc taaatccttc ccagaaaagt atgccaatcc caagaaatat tcaatcaaat 22740
tgctggaaag aaaaatacaa aatattaaaa tgtattagga agcgacagta attaaatcag 22800
aactggagca ggaatagacc agcagatcaa tgagacagac atcaagtccc ggaatgtgga 22860
cttgcaaatg cattaagtaa tatgatatgc aataaaggtg gcacagtgaa ccaatgggaa 22920
aaaaattaat cttataataa ttgatattgc aataattgtc tagtaattgg gggaagaaat 22980
aagcttattc cttatctcat ttcttttttt ctttttgaga cagagtctca ctctggtagc 23040
ccaggctgga gtgcagcgat gcgatctctg cccactgcaa ccttgctctc ccgggctcag 23100
gcgattctcc cacctcagcc tcccgagcag ctgaactaca ggcgtgtgcc accactcccg 23160
gcaatttttt tttccatttt tagtaaaaat ggggtttcac catgttgcct gggctggtct 23220
tgaactcctg ggctcaggca atccacccgc cttggcctcc caaagtgcta gcattacagg 23280
catgagccac cgcgcctggc agctcatttc ttagactaaa taaattggag atggctaaaa 23340
gatttctatg taggccaact atgtttttaa aaagtttttt ttttaaggat atctgctgga 23400
accaatcatg ccaccaacca aagatgcaag actataaaac atacccagtt tttcaaagca 23460
tttaaaaatt attctaaaaa tattttttct ccagaaattt tgcattgatt ccctgaagaa 23520
gcattaatat gggacctgac ttataaaatg atgaactcaa tctccccact caaggtagga 23580
gtctctcaga tttaaaaaat aagcatccta gtcctcttgt ccctgtaaaa gttaaccctt 23640
acacctgaaa caccaggaga ctggcggttg tttgcatagg ggttacaatt aaagttgagc 23700
tacctctgac atctattaac accaaaatta gtaaactatg catgtatgga gacttttatg 23760
attgaacttg tttattgagt caagagatat agtttacaat gaaaatttgg ggcatatcaa 23820
aatgaccttg gcttagctta gcatttgctg atgttaacta ttttcttcat tgggctgatt 23880
ttagttgctt aggaaaaata caaacacaca cactttaaaa ttatattaaa atcccgtcct 23940
aaacctcaga gtccagaacc gcatcctaac actggtcatg cataatatgt ttaaattttt 24000
gtgctttaaa aactacaaat aaggaatgta ttaatagttc cacaatcaat ggtcagttag 24060
ccgagggaag attagcatag ttaaagactt aaaatggctt tacaacatat atcaaaagga 24120
caaaataagg ggaacagagt ctagaaatga ggaaactggg acacaggcaa aaaaaaaaaa 24180
tgagaactgg gacatgaata acgcaaggga taagactaat acacaaaaca ccccaaataa 24240
atagccagca tttgctgagc tcttactgtg agcctgttct aagcacttta catatattaa 24300
ctcatttcat cctcaaggaa ccatctgagg caggcactgt tatcatctcc attttacaga 24360
taaggaatag acccagagag gctgagcaac tgggcctatt ccacagctac tatggtggag 24420
atgagattta aatctaatca ttggctccag agcccatgca cccaatggct gcactaagtg 24480
aatgcatgcg ctatcaacgt tgccaaaagt gggccacagc tcggatctgc gttttccagt 24540
agccaaagca gagagtgtga tcagacctca ctttaataag caagtctcaa gccagagaga 24600
ggtggtatca ggcagcaaac aggctgctag tcgaaatccc acttcttctc tgagtggtcc 24660
atacagtttt actctacttg cttacagaat gaaaatagct ggagttcagg tgcgctttca 24720
atgccctgtt gtcaggattg ggcttttcaa gtttattttt tgttgttgtt tttaatagac 24780
tgtacttttt agaaaatttt tagatttaca gaaagattga gaggatagta cagagagttc 24840
ccgtatacct cacacccagt ttctgcaatt attaacctct tacattcatg cggtacattt 24900
gttacaatta atgagccagg gccggccggg cacagtggtt caggccccta atcccagcac 24960
tttgggaggc agaggcaagc gaatcacttg aggtcaggag ttcgagacta gcctgaccaa 25020
catggtaaac cctttctgta ctaaaaatac aaaaaattag ccaggcatgg tgctggttgc 25080
ctgtattccc agatactcag gaggctgagg cacaagaatt gcttgaacca gggaggcgga 25140
ggttgcagta agccgagatc gtgccactgc actccagcct gggcaacaga gcgagactcc 25200
atcaaaaaaa aaaaaaaaaa aaaaagaagg aaggaaggaa ggaaaattaa tgagccaata 25260
ttgagacatt attattacta aagtccatgc tttatgcaga ttttcttagt ttttacctgc 25320
tgtcattttt cagttccagg aatgcattca ggatgccata ccacatttag ttctcatatc 25380
tgcttaggct cctcttggct agactgagtt ttaatctact ttctgcagag cctgagaact 25440
ttagcataat ttccttggaa attacagctc aatattttca agcacttata caaacagcct 25500
aatgttacgt tggcccataa cagtgtttca aggtaataaa cttctttgtt ttctgtgccg 25560
attgaaagaa ctgctgctta gcctcctgcc agatgatgaa ctgggtacac acgagcattt 25620
ttccaggtaa agcatatttc gtgcgacttc ttaagctgca gccttatatg caataattgt 25680
ccatttacaa gacttatgtt cgaatttcag gcactctgtt ttcactaacc atatccttca 25740
actttgataa gtactgcttt aatcaactca gaaaatttaa cttgactaat tttttttcac 25800
catcagtttt ttttctgttg actctttctc ctttttctgt ttgcccagaa acatgctcag 25860
gattctctca ggctttaaaa aatgaaaaaa tgtttcctgc aatctagtta ctccttgatt 25920
ctcttgttct gtttatcgct ggaattcttg aaagcttggt gtattagtct tttttcatgc 25980
tgctgataaa gatatacctg agactggata atttataaag aaaaagaggt ttaatggact 26040
cacagttcca cgtggctgag gaagcctcac aatcatggtg gaaggcaaaa ggcatgtctt 26100
acatggcagc agacaagaga gaatgagaac caagggattt ccccttataa aaccatcaga 26160
tcttgtgaga cttattcact accacaagaa caatatgggg taaaccgccc ccatgattca 26220
attatctccc accggggccc tcccacaaca cgtgggaatt atgggagcta caattcaaga 26280
tgacatttgg gtggggacat ggccaaacca tatcacctgg cctatagcat tatttccatt 26340
tcttccccat ccttttattc ctcaaaccgg tacaaccaga cctctttttt tttttttcta 26400
cctgaaactg ctcttttgag ggtagctgat aagtccaaaa tactgtcacc ttttctcaat 26460
tccgttcctt cttatgcctt tggagcaatt gactgtgttg gttgccccct cctttaaagt 26520
gtctctcact tggttttatg actaatgatg attttctttt tcctctctaa acattccgct 26580
atctttttag cttcccttcc ccctcccatc ccctaaatgt ccttgtttcc cagaatctgc 26640
ctcacctctt tgacttctct atgccctgtc attcactcat gggtctttat tacattattg 26700
catctgtgtc aataactctg gtctttctgt taagttccag tctcccattt tcaaatgtcc 26760
ccagacattt ccaattgagt atctctccaa tgtatttaac ctgctaaata tctaacacat 26820
aatctttccc atcaaatcgt ttcctcttaa gcttttcgtt atttcctatt agactcctgc 26880
acttctccca ggagcccaga cttaaaacct tgaatttctc accataacct ctcttttgtc 26940
tcccataatc aattagtagc aagtgttatc aatgattact tgacaatatc tttttctatt 27000
tccctccctg ctatgatcat tcatctagca agaagagttg gccctttgta tctgtggttt 27060
ctgcatccct ggattcaacc aactgtagat ggaaaatatt tgaagaaaaa agcgtctata 27120
ctgagtatga aaaaatttta tttcttgtca ttattcccta aacaatacag tataacaact 27180
acagcattta cactgtagcg tatagatctt ataatctaga aatgatttca agtacaccat 27240
tatatataag ggacttgagc atctgtgaag tttggtattt gtggggcata ctgggaccaa 27300
ttcccccatg gatacagagg gacaactata tttactcagt gcttactaaa taccagttgg 27360
ccaatgtgtt tttctttttc tgttttcctg tctttagttt gccccttgcc aattaattca 27420
atagtgctgc caatgccagg tgtaccttca gaatattcta ttctaatttt gtcatctcca 27480
agcttaaaaa tatttaatgg gccaggcgca gtggctcaca cttgtaatcc cagcattttg 27540
ggaggccaag ggggggtgta tcacttgagg tcaggagttc cagaccagcc tggccaacat 27600
ggcgaaaccc tgtctctaca aaaaagtata aaagttaacc aggtgctgga gcatttgcct 27660
gtggtcccag ctactcagga ggctgaggca ggaaaatcac tttaatctgg gaggtggagt 27720
ttgcagtgag ccaagatctc tccactgcac tccagcctgg gtgacacagc aagactctat 27780
ctcaaaacaa caataacaac aacaacgaaa aacatttaat ggctgcacct tgcctgtgaa 27840
aaatgcattt cttggccaga tgtggtggct caaacctgta atcccaacac tttgggaagc 27900
taaggccagg agttcgagac gagctgggat atataggaag acacaatctc tacaaaaaaa 27960
aatccacaaa attagtcagg cttattgttc atgcctgtag tcccaggtac tcaggaggct 28020
gaggcaggat tcctcaagcc caggagttca aggcttccgt gagctatgat ggcacaactg 28080
cactccatct tgggtgacag agcaaggtcc tatctctgga gaaaaaaaaa aaaagaaggc 28140
atttcttagg agagttcttc tctgtagagt cctaagggtt ccatggaact ccttaaaagc 28200
atcagagtat gtgagtgcaa tgggaggaag catttagcca gagcagttgt gctcccattg 28260
catattaatt tttaaaaaac aaagctataa aaaaaagttg aaaactacta cgttagcatc 28320
agcctgacat ttaatggcct cgtaaatcaa accttaattg actttttagc cagttatgct 28380
actagccaac tacagacaac acacttttta accaaattag actaatagtt gtcatcagtg 28440
gaaatcaagt ttgccattct tccatgcctt tgctcacacc attacctttt ctggaatgtc 28500
ctgtactcat cttcctgtgt tgaactctat acccaacttt aaaaacctag ctcaaagttc 28560
aacacttcca ttccatttca aaaagagctt tcctcttcct taaagtttaa gaactcattt 28620
tcatgaatct ttttggcatt tattgcacac atgcttgctt tgtgttattt gtgttcagcc 28680
tcatatgccc ccaaggtgtt ttagactcct taacggcaaa aatgatgctc taaacacctt 28740
tctatctttc atagtgtctt agtctgtttg tgttgctata aaggaatacc tgaggctggg 28800
gaatttattt aaaaaagagg tttatttggc tcacagttct gcagctatat aagaagcata 28860
gtgtcagcat ctgcttcagg tgagggcttc aggaagtttc cacccatggt agaaggcaaa 28920
ggggagcagg catcacatat caagagagga ggaaaaaaag gaaggaagaa aggagggtgc 28980
cattctcttt caacaatcag ttcttgtggg aactaatggg acaagaggct gggcacggtg 29040
gctcatgcct gtaatcccag ccctttggga gaccaaggtg ggtggatcac ctgaagtcag 29100
aagcctgaga ccagcctggc caatgtggtg aaactccgtc tctactaaaa atacaaaaat 29160
tagctgggcc tggtggcgtg tacctgtagt cccagatact caggaggctg aggtaggata 29220
atcacttgaa cccggaagac agaggttgca gtgagcttgt gccactgcac tccagccggg 29280
gcaacagagt gagacggtct caaaaaattt taaaaacttt aaaaataata gagcaagaaa 29340
gcaccaagtt attcaggagg gatccacccc caatgactca aatacctccc accaggcctc 29400
acttccaaca ctggggatca atttccgtat gagatttgga ggagacaaat atccaaacta 29460
tatcacatag taatgaacat agtaccttat ctatagaaag caatggctag acaactgttg 29520
aatggctaac caaatctgct ttcctatggt ctcgctctag agggggtcag tatgagtttc 29580
tgtcaaaagg agaaaaaaaa atgtatagtc agttttgtgt gtgtgtgtgt tcatgtaaaa 29640
gagatcaaga gaaaagaaca agagaaatca tgaaaaggag ggggaatata agaataatac 29700
atagaaaaaa gcaaattatc ttgtttatca gtaataccca agggggtaga aatggtaagt 29760
aataatcctt cttcactttg tctgtagttc acttttttgc acctttattt tgatgaattc 29820
acatcgaaga cattaactca ttaaggcttc caatattttt ggagataaga agggctgcta 29880
tgctctttat agatggaaaa cttgggtcat taataactca aacaaggaca taacaaagaa 29940
atggagcata aactgccagg tcctgactgt agatttggat tcccagttgg tgtcttgtca 30000
ccctttgtta ctcttcctaa agttatgatc ttttcttgtg cataggaaat tcatagtgat 30060
ttcccatcac ccttgggatt atcatagctc ctttaaggtc ccctctatgc actcaataac 30120
atcaacagta agtgttcttc gagcacttac tgagtgtata tcattgtgtt ctcacgcagc 30180
acccacagat ctcaccaaga acctagctga agcctgtaga atgaataggt aagtactgcc 30240
atgccaatct ggagtactca agcgatgcaa atgattcctt taattgtact tttgcaggct 30300
tgtcagtttt gctcatggag aagtggctac tgcatccatg ttatatctat gtaatgttgg 30360
actgcgaagc atcacttgac tttttccaag cagaaattac agctgatgac aagctgctgc 30420
tgagaaaatg gatatttttc tgaattcagt tctacgtgga aacagctgac tagtttccat 30480
tgctgtaaga atggctcttt tgctcttggt tgattttgag taatggcttt acttctgtag 30540
aaaggagatt tcatttgaag tccactcagg gatttggttc aacaaactgg agtacaggtt 30600
tcagaaaata tctctttaat cctccaataa taaattttct catctataat tcctggaaca 30660
cttcatcctt tgcagccgag catatagata gatttgttgc tcactgtgtt ctgattgcca 30720
ctttgacctg ctttttcaac ttaggttaca aatagaacag aatctctctg atttttctca 30780
ttaattgttt gaattcccac ttttcctcat tagcaagaag tccagtatct tcctgagaac 30840
ttccttttct caatctagga acttacttgg tccataaggt aacagtctta tttctgacta 30900
tcaaggagag aaataacagg agccattatc atcttcatgg tgtcactttt gaaaactggt 30960
cctctgtaga tcttcagatt cttgcgttag tccattcagc tgctataaca aaattgcata 31020
gacagcatgg cttataaata acagaaatgt atttctgaca gttctgaagg ctagaaagtc 31080
aaagattaag acactggctg atttggtgtc tggcgaaggc ccatttgctc atagatggac 31140
gatgaccttt cactctgtct gcacatggca gaagggcaag agagctctct gggtcttttt 31200
tataagggca ctaatctcat ttttgaggac cctgccccca tgacttaatc acctcccaaa 31260
ggcactgtct cccaatacca tcaccttgag ggttaggatt tcaacatatg attttggggg 31320
gacagaaaca cgcagtccat ctcgcttgtc cactccatgg tggtattctt gctggatcag 31380
tttcctcctt ggggtgcatt tgtgttccat gtctaacttg caagttatag caggcccgat 31440
agcaaagtat tccaatgttg gtatgcagag gcattgaata atcagaatga acccacgcca 31500
taaacaactg gtagagctgc agagagtacc agctgattat gagccctggg taacagtggt 31560
ttttagttcc tatgtccgtc agcccttttc tcccatagta gccccactgt gttgaagtgg 31620
ctgaatcgac agaagcttcc agcttgggcc acatgctcat ggaaccaatt ctccttatga 31680
gccgtacaag agctgggttg ccattctgga taccctcttt tttcaagaga ttttatttca 31740
aggatatttt ttcttttatc aactacaggg attatttaga atcttagggc agtggtgccc 31800
aacctttttg gccccaggga caggttttgt gggagacagt ttttccatgg accagtgtca 31860
gggggctggg aggcatggtt ttgggatgag tcaagtacat tacgtttgtt gtatacttta 31920
tttctattat tattatattg taatatataa tgaaataatt acacaactca ccataatgta 31980
ggaatcagtg gggagcccta agtttgtttt cctgcaacta gacagtccca tctgggggca 32040
atgggagata gtgacagatc atcaagcatt agattctcat aaggagtgct cagcctagat 32100
ccccggcatg tgcagttcac aataggattt gctcacctat gagaatctaa tgccactgct 32160
gatctgacag gaggtggagc tcgggcagta atgcgagggt tggggagcag ctgtcaatat 32220
agatgaagct ttgctcgctc gcctgccact cacctcctgc tgtgtggtcc acttcctaac 32280
aggtcacaga ctggtactgg tccatggcca gggagttggg gaccctgtct tagggagtag 32340
gggtggagtt cccttcactt ctagaaggcc ctggattagt atcccagagc tgtcattaca 32400
gagtatcaca aaccaggtgg ctaaaaacag acatgaattc tctcttattt ttgatggctt 32460
ggaagtccaa agtcaaggtg ctgccagggc catgctccct ctgaaatgtg taggggagaa 32520
tccttccttc ctctttctag cttctggtgg tttgctggca atcactggca tcgcttggct 32580
tgcagcactt caacatctgc ctttactgtc tcatagtgtt ctcccctcat gtctccaggt 32640
ctctctgtct ctcttctttg tataaggaaa ctagtcatat tggattaagg gccaacccta 32700
ctctagtatg acctcatctt aaggtcacat gcaatgacta ttccagataa ggtcacattc 32760
tgaagaactg ggagttagga cttcatatct tttgaaggaa cacagttcaa ccaataacag 32820
cccctgtact gttttacaaa taggtattcc tctccttccc aaagttcttc atagcagaga 32880
caacttgtac caaaaggcaa aataccttat tatgtaacct taacctagga tcatagatcc 32940
ctactgtctg gtgctttata agcacagaac caccgggaaa tcattattaa gacaaggaaa 33000
ggccaagtgc agtggctcat gcctgtaatc ccagcacttt gggaaattga ggcgagtgga 33060
tcaacctgaa gtcaagagtt tgagaccaaa ctgaccagca tgacagaacc ccatctctac 33120
taaaaataca aaaattagtt gggcatggtg gcatgtgcct gtaatcccag ctactcaaaa 33180
gactgaggca ggaaaatcac ttgaaccgag gatgccaaga tagcagtgag ccaatatcgt 33240
gccactgcac tccagtctgg atgatagagc aagatcctgt ctcaaaaaat taataaataa 33300
ataaaaagac aaggaaagcc ttttccaagg agacccttct gctttgctag ttcagagaac 33360
ttctcttttg gagaaaacaa acacccagtc cattagcagc aacgtcaggg attgaattct 33420
tagggcagca ggctgggcac agtggctcat gcctgtaatc ccagtacttt gggaggctga 33480
gatgggtgga tcacttgaca tcaggtgttc gagaccagcc tggccaacat ggtgaaaact 33540
catctctaca aaaaatatga aaaaaaaaaa aaaaaaaaaa gctgggtgtg ttggcttatg 33600
cctgtagtct cagctacctg ggaggctgaa gcaggagaat cacttgaacc cgggagttgg 33660
aggttgcagt gagctgagat tgccctactg tactccaacc tgggtgacag agagagactc 33720
catctcaaaa aaataaagaa ttcttcgggc agcagtcttt cctccacctc atagaccatg 33780
gaggtgagcc agctctgaca aaccatgaga acaatggcag agacatacct gtaacgtaac 33840
tgactggggc aaagacaaag gtgaggaaaa tgacaagttt gaggaactat gagaccaggc 33900
agtggggaac accactagca gaaatgatgg aagttctcaa gaataacaac agagaaatag 33960
accatggcca gagtctagaa ccctccaggg aaaggagatg ggctccagag gcagaagagg 34020
acgttgaagg gaatggggag tgggtgaaat atatagacga tggggaccac ccaagagcag 34080
tcgctattgc aaaactgagg agaaggagag tctggagggg gtggtgggaa gctgggtctc 34140
ctaaggaggt tttgacaaaa gcagtcatgg agcgggctta gaaatcacag ttggggacag 34200
ggtaaagttc ctcgggatat agaggatgag attagaagag gttccaacta gggtagtgtg 34260
gagaaaagca ctattgaccc aaaaaggaag gagaatgtgg gtggaagtgg cagagaaaga 34320
ggggtttgag cagagagtgg tgatttttct aatgcagagt tgtgggaggt ggagtgcagg 34380
gagccaggct gggtggctgt gctgatgtga ttaagcactt actgactgcc aggcaatggg 34440
ctaagtacct gagatgcttt gtctgttatc cctcccgaaa cccctctgag caggtgcagt 34500
tattattctc acttcacaga taaggaaatt gaggcacaga gaattgagta acttacccaa 34560
ggtgacatag ctcatatatg gtaaagcagg ctttgaactc agtctagctc ccgaacctaa 34620
gcttgtaact actatgcttt tcccaaaaaa agggggctgg cacaaaaaga gctgaggggg 34680
ctgggcatgg tggctcatgc ctgtaatccc agcacttcgg gagactgagg caggtggttc 34740
accagagttc aggagttcga gaccagcctg gtcaacatgg tgaagccctg tctctactaa 34800
aaatacaaaa attagctggg tgtggtggtg tgcacctgta gtcccagcta ctttgggagg 34860
ctgaggcagg agaatcgctt gaaccccaga ggcggatgtt gtagtgagcc aagatcatgc 34920
cactggactc cagcctgggt gacagagtga gactccatcc aaaaaaaaga agagctgagg 34980
tgatggccac catcagcatc agcctggaag ttatagcagg atgctaagtt tctctaaagc 35040
tgtctttctt aggacttgaa aaagataact tgggtttgta tcccatctct gccattagta 35100
gtttactggc tttggataaa ttacttagcc ttactgaacc aactttggat ttttatagag 35160
atactgtaat gaaaggaata aggtatcagt cttagcagag catccagagt gttcctatta 35220
aaacctaaat catatcctgt cattgctgtg ccccaaacca ttcaatggct tcccaactca 35280
aagttaaaaa ctcatctttc cagtggcctg caagagccta tgctatccgg tgtctgacct 35340
catctgttgt tcctttctcc ctccctttct tggctccaga cgcactctgg tctccttgct 35400
gttccttgaa tacaccaggc acactctctt cgcctgaaac actttacccc agatatctta 35460
gcttactctc tgcctccctc aattcattga tgaaatgtct cagtgaagtc ttctctctct 35520
cctctgtaaa agtatactct ctgttcccct tctttactgt tctagctact attgctgtgt 35580
aacaaatcac tccccaaatt taatgagtga aaacatcagc catcatctta tttctcacgg 35640
tttctgaggg tcaggaattc tggaagggct cagctgggag gttctggctc tataatctct 35700
tatgcagtga gagtcagatg ctggctaaaa ctgaaacaaa gcagggttct agtagctgag 35760
ggctggctgg gtctctcaga tatagttcag atctcctcca gggggtctct ccacgtgggc 35820
tagtctgaac ttcctcacag catggtggcc tcagggcagt ggactctgca tagtggctga 35880
aggcttcgca gctgagtatt ccagcaagca aagtgggagc tgtattgcct catatgaccc 35940
aaccttggaa tccacacagc atcacttccg tgtattctac gggttgaaaa gtcacaaaaa 36000
ccaaccagtt tcaaggagaa ggaacagaga tcacatttct caattggaga agggtcaaag 36060
tcacattgta atcagagcct atgggatacg aagtattgcg gtcaggtatg aaaaatttga 36120
tttgctgcat ctgctttact ttctccacag cgttcatgat ctgcttctca catgatattg 36180
acttacgtca tttctgcgtt tcctgtcttc cacactaaaa tgtcagcctg ttttgttcac 36240
tgctgtatcc ccagagccta gcacggagcc cagcatgtag tggtatccaa taaatacttg 36300
ttgcatgaat gaattctgtc ttttaatcct agctataggt ttctaagtta aatattacta 36360
taatcatctt acagacgagg gaaatgaggc tcaagaagat ttggtaactt atgcgggatc 36420
actcagccac ataatggaag agacagcatt gaagtacaca tgcttgctct gtctgctctt 36480
ccaagctgct catcacacag ctgcacctct gaggacttcc ctccccagtc cacctccacc 36540
cttacccaga gacacacatg gccacaatcc actagcagac caaaattcaa tttttcccca 36600
gttggttgca ctcaagctga gagcaaagca attgcacttt aaatcccctt acagcagata 36660
tttcagagca tgttcggaag aacccatcac acttggcttt tagatcttat ttctggtttg 36720
ttacaaaaac acaattaaat gaaaggttag gtagcttttg aatggccagc tcaaagtttt 36780
ggcttatttt tgccttgctg tctttatagg cattttacca atatttatca ctatttccct 36840
tagggaaccc ttagatctgt gatatttgaa ataataaagc ctctccattg gccctttaaa 36900
aggtttgtgg taaaaccaca ccattaacat tcacagttcc ttatttatga ggcctgattg 36960
cacttatttc catatttctc actgtttctc cgatgaggat ttcacataat agtgtttgaa 37020
ggctaaagac ttcaaagcag attctttact atttttatct tgaaaaatat tcaatatttg 37080
tgtaattaaa gtgaagtctt cctagagaaa atgacaactc aaataatctt aaatgtacct 37140
ccaagaaaaa agctgtcaaa gtgacattta gtaatagagt cacattctct aaggcctttg 37200
cttctccttc tgattcttat catctttgaa ggttatgtca tgggctgact tcaaatcaac 37260
ttttaaaatt attatggcct tctttaaatg tgagttctga aggtgagggg ctttatcttt 37320
cttttgctcc agattttttt taccgcgtca ttaccaagca tcttaaaaca aaacctaaaa 37380
acaaaaatct tccttgacct ggtttttccc actagctaac atcctatttt tatctttccc 37440
ctttgcacta aaggttttta aacggatctt tataccctct gtctccattt tctcatctgc 37500
taacttatat ggcaaagatt accactgcct ttcaacataa ttggccaatc tacagaaagt 37560
tttcaagttc tctttttaat tgaccacctc ctgcctacct ccccaccttt gacatcttgc 37620
ttctcacttg gcaccttacc cagtgttcaa gattccctcc tttaggatgt cttcagagca 37680
gctacacagt tggtactata atttatacat ccttgtacac agggcttgct gggatattga 37740
tggagagaag gaggaaactg gaagtagttc aggccagagc tagggaaatt gacccatctc 37800
caggtctcag gtctgcaagg ggagctcaca gcttaacaca tggagtctag aaacttgtgc 37860
tggaccttga ccaacaccag cccatggagt ccaatacagt gctcaatagg gatttccagg 37920
aaattgctat atttattcaa agagaactta ccaagtgtca gctacgtgtt gggcattgtg 37980
ctaggcacag ggaccacaaa gataagacat tgtagctttc cttaagttgc tcactgagta 38040
aatagagaga cagaaaggta aacaggtaag tgcaaaaata catacaattc agcaatagtg 38100
ttcatagtgg ctatggagag aacgctcact aactttgttt aaacagttgt tctttcaagg 38160
atttgacatg gatttgattg gaaaagcatg ataccatttt ttgcaattaa acacaggaat 38220
acataaataa aatgcatcag tattttttac aaatagctac taagagctac tagaaaacct 38280
gggaattctt aaaaccttac catgctactt gctctaaaat attttatttt atgttatttt 38340
gtacatttct ttacctacac aaacaccact gttttcttca tttcttagtc tatttaaacc 38400
tcacaccctt tcagcatctc ttaattattt actaccatct gttagttctc ctgtcctgaa 38460
tgaaacaaaa atggcagaat gtaaaacgag ggcgaacaga tttttgacag gaagtattca 38520
gaggtagaag gaaatagtca agacacatat gataaacgaa aacaataata actttataca 38580
taacaactta tagacacatt taaaaagttt aagatctcaa gagctatgtc tgaatagata 38640
ggagtaaaaa ctctattaag taattaggaa aataacaaga acagtgaatt tcttaatgaa 38700
tggcatgtaa tcaaaactgt acttatcgtc taattcataa tcttgaatgt ttttatttta 38760
tttatttatt tttttatttt ttgagacaga gtcttgctct gtcacccagg ctagagtaca 38820
gtggcgtgat ctcagctcac tgcaacctcc acctcccagg ttcaagcgat tctgctgcct 38880
cagcctcctg agtagctggg attacagagg cctgccactg cacccggcta atttctgtat 38940
ttttagtaga gatggggttt caccatcttg gccaggctgg tcttgaactc ctgacctcat 39000
gatccaccag ccttggcctc ccaaagtgct gggattacag gcgtgagcca ccacgcctgg 39060
tcgaatgtct ttattatttg aagagacaac atgggcctta aatctgtctt ctatttgaca 39120
gactttgatg gagtcaaatc ccaatgctgc cacttactga acggccttaa atgacttagt 39180
ctctctcagc tgtctttctg catatgtaag gtggaataat gatggctttc aaggaggaat 39240
aaacctatga aaagtgttga ggatagtgtt tgatatgaaa taaggatttc aacaagtagt 39300
agctgctatt gaagatttaa gagttattta ttacaactat ttaataaaat tttaaaaact 39360
aatacactta aattattaaa gagctttgaa atgggccagg cgcagtagct cctgcctgta 39420
atcccaacac tttgggaggc caaggtgggc ggatcacctg aggtcaggag tttaagacca 39480
gcctggccaa catggtgaaa ccctgtctct actaaaaacg caaaaattag ccaggtgtgg 39540
tggcatgcac ctgtagtccc aactactcag gaggttgagg gaggagaatt gcttgaacct 39600
aggaggtgga ggttgcagta acccgagatg tcactgcact ccagcctggc aacagagcaa 39660
gactccataa agacaacaaa agctttgaaa ttgtgtaaat gagttgtacc tatcttcatt 39720
taagaaattc atctttgttc atttattttt acttgacatg agagcttcca gcaattttta 39780
attaagccct cacagatttt atgtcactgg ctatgtgata aacaaattat ttgctaaaat 39840
aatattcttg cttctttttt aaggaattgt ctccctagaa acggtttgta ccaaacaata 39900
cactgacttt acacaaaatc agatctgatt ggcaacagtt gcagatgttt tcaaaagatt 39960
ttcatttgag aaggggccca tttgggttat ttagattcta agaactgaaa ctgctttgtt 40020
ctgtttttct ggcttctggg agaggaggag acatgaattc agttagcacc ttggtatttt 40080
ctttatcctt catttcaata cagaagatgc ttcatatgca cagtggtgtc aggtcacatc 40140
aaaagaaaga gaaacagttt cttggttttt aattttcaac cggaaaggaa aggcacccat 40200
tttgttccgc tctaattagc cagtgcatga cttagagagc aggcagatgc tttgaaggcg 40260
tggtaacaca ggtcttcatt aatctccacg caggacttgc acttctacta tgcctaggct 40320
gaagaaaatg gctcaggaag atgaacaatc tcacagagcc ctaactaact gaagccaggt 40380
gttataaagc acaagtcaag agggtgagaa actaacgttc ttgaaatctc ccacttcttt 40440
ctacgtcaga agagccaagc tgattatttt agttggaatt tagaaatttt taaaaattat 40500
tctaaagtca tgaacaagcc taattataaa gatagttgct gtgaaggtgc tgaaataact 40560
cgattttacc aaccccctct tctggaggaa gccataatgg aatcctgtac aatgttcact 40620
ctaccaacga actcttgttt ttctaatgag gaaacagagg cccacagtat taaactatct 40680
taaccaatac aaaatgacta gtgctctggt ccttttatta agcactaaaa ttttgatcca 40740
ataataaatc tgtccattag aaggagtttc cctaatgtac tggttctaac ttgttccctt 40800
caaggggcca gtgtcccgta cacatagcta aatgggactt ctcttcaact accattaccc 40860
agagggcaga acctaaaatg ctgtgaatga cattctgctg ttcacatctc agcagcagtg 40920
ttgcatttga gcttctgcag ggccacccag gacctatatc tgctcagatg tttaactcat 40980
ctaattcagt gaacacttca ttctagttaa ctgaacatct actttgtaca aggcactaca 41040
gcggttcaga gatgaataaa atcatgagat tccactgtct cctataaacc atcactttgg 41100
gaaattttag aaatgtgggt aagctccagg gcttcctgca gcgtagaagt cacaaactca 41160
aatgcctgca gaggcccagc tgacaacata agtaaatgat tctggctggg cggaaaacaa 41220
ttacgggtgg gtgggtttcc agctggggag tgcacgcctg tgttaaagga cagctgctac 41280
tcatttccag ccaactgtgt tcccatgtag aactgcggcc cagtgtagcc agtaccgaag 41340
atttctcaga aaaagccgga gatctcaatg ttagtgtaaa atctctcaaa tttccaagag 41400
gattatatgg ggcaaaggtt ctcagatcag tttgcagtct cttacttagc ccatgtgcag 41460
agcagtcgta gagggtagca tgcagtgtcc tacataataa ttctttttta ttttatttta 41520
tgccttcctc cttcctgtct ctctttaacc tttcttcttc cctcaggctg gcttcttccc 41580
tcagcctcgt ccgaccccag cctgggttca atgaacattc ggtaaaggaa cacggaatgt 41640
caagcgcatt agagacaacc ttgagacaca ttcctcttgc ggtaagcact tcactgtaga 41700
tttttaattt taaacaagac aatgtttacg acttgcttct ttcagggaag agcgatatca 41760
attttagtga acacttcaag gctgagatac gctaggagag tcgtgtggtg ttgcacagca 41820
aagaattcca ctttgaagcg agtgggaaaa aaagcatcaa atgccacatg taactcaccg 41880
cctgaagggt tacattggta tgaaacctgg gtttaaaaag ggaccgaata gactagccat 41940
taaaagacct gcgtacaacc tctctctctc tctttgagag ataatgtatc tggacaataa 42000
acatgaacag agtggagtct atcctgttta aaacattgcc tactgtacag gcaccaggag 42060
ctgaagggtc agaatattag cagtgggagc ttgattagag ttgatgagag atgggtagta 42120
ggaggaaaga gtgagataga ggaagaggac atgggggtta cccataagtg gagagtagaa 42180
aagtagaatc agctggccat caaagggcgt gggactgagg aacagtatgg catgtattaa 42240
atatactaag cgctgacatt ggaggagaac taggaagtta aatgaaatca ataggggatg 42300
atggagaata gttaggtgtg cagggattag ggttatgata gaaatacatg tgaatacatg 42360
cagtattgtc ctggaaaatg gttaacagtt ggttctcctg gggggtgagg ggaagccctg 42420
atttgtaata tttgcctatt tctgtggtgc aaatactccc accatgacca gtttcaagct 42480
atgaatgttg aagtcacaga aagcaggttg ggaggagatg cgcacatttg ttccccggca 42540
aggtggaagg taaggaaggt gaaatcaaca aggtcaaaga aaactcaaga tttcgaggtg 42600
cctcaggtct gaggggcaat gaagtctagg aatggctgtg ctgaggtagc tgaaatagaa 42660
gtgactgcag aggtcatgaa gctgaagagg tgaaaacaga aattagaaag gcaaaccccc 42720
accgcccaac ccccacccct gcagccagtt tctgagggtg acaatagagg aaagggtgga 42780
gatggagttc aggtccagaa gccatagaag cgagtgtgac attgtgctca aggtcagcac 42840
atgtcagtgt ggggtgtcac atgctgttgt gaaccatcat ttatcaccaa ttatggaaga 42900
cctcctatgg gcatcttgcc atatgcatta taaagatgtg taagaagaca tttccctcca 42960
cttggtgagg agaattaggg ctgtacacag atactgtaga gtgccatgtg cctggtacag 43020
ataaggtgtg ttagaggtta aaagatgagg ctcttaatat taatgataga tcccacttac 43080
ctgagtctga cttacaatgt gcctagcatt aagtgtttta cctgcattcc ctttgacgtt 43140
cagaacaacc cattttacag atagggaaat tgggtcagaa agtttcagta acttatccaa 43200
ggtcaacaca attggcaagt gccagagctg agccaggaac tgaggtcctt ctaacaccaa 43260
acagcttgtc tccccaatca ctgtgctatt ttcctccccc agaagataat actctgatgg 43320
aaatgaagga tagtgtaata ggagattcgg tgttcctttt tttaaaaaaa attcagcttg 43380
catattccta aagagtcaat tcatgtttaa aaaaaatttc ccttgtgctt gcatgtgaca 43440
tgtattttta ggatctgctg ttagcaagtg tatttttgtg tgattgagtg ggagagtggg 43500
aaaagttttg cagagctgtt gaagccagaa tgcagggggg ctgcgcagca gagactgtaa 43560
aatctctgcc atctcaggtc ttggaacaag cacaaagaga tgtgttctcg atttattatt 43620
ctatgtacat ccccagatga atgactagtt aaaggtattg ttaaagcatt ttaaatgacc 43680
cacttccagc agcgaacaaa atcacttgct gtgccaagcc aactggcatt tctgagatga 43740
taaaaccaca aagtgaggaa aacgttaaaa ctgctaaagc aaaaatgata cacaataatg 43800
gagaaggaga aaaattgagc tttattgtct gcctaggcag atggctgacc actaggtggg 43860
cctcggcgtc acgtccaggg taattggttg ctggggtgtt tctggcgagg aagattcacg 43920
cttcagctcg gtccacaaga tcctggctca ttctttccta gattccattt tctgcctcct 43980
ctccatgact gggtctgatg gttgatccaa acgggcaatt gaaatcagaa ggttaccttt 44040
accttaaaat gcttttctgg aaataaaagg acatgaaaag taactaagga ccggatttcc 44100
tagccgtctt tctctcctgc atgcgcaatt tatccccaga tataaaattg cctgctttga 44160
taattatacc ctctaaatga ggggcaagtg gctaattatg cccacatgtg gccgattgca 44220
ctccccatta gccaattatg tgctcaatta tttgtgcaca tgaataattg cactcatgga 44280
aaatagcgcc ctcctttcaa atcctcgtgc ttggagtggc tgatggagta attgtcacac 44340
tggaaatgca cttggtgggg agggaaagag tatcagatac caggaaacgc ataagtgacc 44400
agagctcgca gatgttcact gccacaaatg gccttaggag ccagagagag cgggaaggac 44460
cacaggatgg aacgggccag cctgtgagtt aggaagcctg cttctgaagt tgcctgggca 44520
gctcatgtgc ggtgaccttg ggcaagtcat taactttcct tcaggtctaa ctggttctgc 44580
atacacaatg aggatggtaa taacgcccaa ttcccatcac tatcgtggga tggatcagac 44640
tatttaaaag gatttacaat ctgcttgggt aaaagcttta cataaatatg aggcattatc 44700
atgtcgcttg gtacatctcc aattatgaag gaagggtaat gaccctccac agcaatgcag 44760
gactcctggt ttggagggag ggaaagtttg agaaggacag gaagcttgtt gccccagcac 44820
tgatgtttct actgaggtac cagaaaatgt catgtggtca tacagaattc atttattcat 44880
tcaacaaaca tctgtcaatt gttacactgt cctgagaatt tggaaaaatg atgaaagact 44940
cagtcctgcc ttaggaggtc actggcacat tggcccgggc ccctgttttg ggccttttac 45000
tctgacctgt gctgatttgc aaatagtggg aaattttatc tcaagtctat gaaatctggc 45060
atgcattttc acggtttgat tgccaggtac attcgatggc aatgagtctt ataatgtttg 45120
gttaccttca tttacctaag aactgtggtt gttgctgtgg ttgttgtttt tgttgttttt 45180
gagacggagt cttgctctgt catccaggct ggagtgcagt ggcatgatct ccggtcactg 45240
caaactccac ctcccaggtt caagcgattc tcatgcctca gccccctcag tagctggatt 45300
acaggcgcgc accaccatgc ccggctaatt tttgtatttt ttgttcggga cacagatttc 45360
acatgttggc caggctggtc tcgaactcct gatctctggt gatccgcctg cctcggcctc 45420
ccaaagtgct gtgattacag gcgtgagcca ctgtgcccag ccagaactgt ggttttaatg 45480
acaatgctaa aaagtggtat atgtcacagt gtcgggtggg gctaagaggc acattgctgc 45540
agtgatccat cattcatttc ccaccattct cgcctggatt agcgcagcag ctcccagaga 45600
ggcacctcac tttgaccttc ttcctcaaag acattctctg tgacctgcct ggcccttatt 45660
acctctctag ctttgccact tccctatgtc tccatctccc ctctcacacg tagtagaaag 45720
agactctacc tcatggagta aggagaggct tcacagaggc aggattgcta ttagtcttca 45780
aagatgaggt atttgctaaa tgaatgagac aaagggattg gggccacatt acagggaaat 45840
tgaggtatgt aatagcctgg tgcaggttaa gagtgtggac tctgaaacca gactcagcct 45900
ggaattgaat cctggctgtg tgatgttggg ccagtgactt aacctctctg tgcttttatt 45960
cactcttcta taaaatgggg attataataa acctacctta taaggttatt ataacagtca 46020
gtaaatataa aaatagaagt ttttggatga tgactatcac atcagtaaac acttgtttgc 46080
cattattttt attacttgac taaaaatata ccaaaaagac catccaagaa aaccctttaa 46140
gctgctagtg cagaaagatt ccccttgtgt ttgtgtgctg gggggtcagt ggtgcctgtg 46200
gcccactgga gaggagacag ctatggctgg agtgattctc aaacttcaga atgtctaaaa 46260
tcatcacatg gacaacttat taaggaaagc aaatgcctgg gctccatcct cagagagtct 46320
cattcactgg gtcaggatag agcccaggaa tctttacctt aaagaaccat cccacctccc 46380
acctcatatg atccttatgc aggtgatctg gggcccacac tttgagaaat agactcaggt 46440
caaagtggct ctaactgcat ctcatttctt acctggcata tctaatagta gagaagaaga 46500
caatgctaag atttttgttg gagatctttt gctgggattg ctgcttcatt cattcactca 46560
tttatttatt tatttattta ttttgaaaca gagtctcact ttgtcaccca ggctggaggg 46620
cagtggcaca atctgagctc actgcagcct caggctcctg ggttcaatcg attctcttgc 46680
ctcagcctcc cgagtagctg ggattacagt catgcaccac cacgcccaac taattcttgt 46740
atttttagta gtgacagcgt ttcaccatgt tagctagact ggtctcgaac tcctgacatc 46800
aggtaatctg cctgcctcgg cctctcaaaa ttagtagctg caattacacg tgtgagctgc 46860
cgtgcctggc ctgctgtttc ttttagttgg gcctcttctg taatagagtg tgagaattct 46920
gacttgctgc aacagtctgc tttgaagcag ggctgtgttt acactggtca gatgtggaat 46980
tgtggggcac acttagcagc ttccttctct aatttttctg tattttcagg agaacaattt 47040
taaaaaattt aataaaaatg ccttaaaaat taacattatt ataagatgaa tcccattttt 47100
ctaatcttgt aaattaaaaa caatcataag catatgagca cctgcactta gggaatcaag 47160
gttggcaaag ctaaacactt ccagctctag gtgattcgcg gcaatacaaa tggagctgga 47220
ctttggccac agtgcaaaaa tattgatctg ttgttagatg ctctgaagtt tccagaaaga 47280
attggttctg cctgctgtgc ttcagtgctt aagggaagtg gttcctcaaa atgttagttt 47340
ttaagcccag ctttcttaaa taggaagatt ctaatagtag caaaaatata aactgcttct 47400
aggtttaaaa aggacccagc acacaatggt tatcacacac ctttctcctc aggtgatgag 47460
tggatgagtg gcctggtgta tttcataaca tctcccaggt ccaaatgcta aagcaattgc 47520
tgaaaagata ccatgtgtac cggaaccttg cagaggtatt ttgttggcat aaaaagaaat 47580
attgatcatc tatagtaaaa atggttctac tttaatacta ctgagaaaag attttctttt 47640
cccagatcta catcctgaat cttcatgaag acaagatccc ctaaacttcc actaacacca 47700
taatgtgtgc tgtcctttgt aatgtagtcc acagatctca taaactgtca gaaatagcag 47760
agattgtaag gtcatccact tcccctgtaa ggcctgcgtc cctcacttac atccctaata 47820
acgtcctcta acctctgctg gagggcagat ttagctgcca gctgggaaga gctctgccct 47880
agtcaacatt tttatctgtg gctttcagat gagaacactg gatgcttatc tgaaaaaagc 47940
tcctcaggct ggagggaggg attggctcta acaagatgca atgtgataag aataaaagcg 48000
aagccaaact ctaggcccaa aggctctagc aacacacttt tgagaacctt ggagacgagt 48060
tttggctgat gcgagcttct ccgcctgcta aagtagccca ttccatttgg acggctctag 48120
aggctggcat gttcttctcc acgttgtgtt aatgtactcc agtttcttcc tgccatgaac 48180
tggcatgccc tggctcctcc taccttcccc actttaagtc ttccctccct ccttctgacc 48240
ttcccattcc agccacactg gccttttgtc tggtcctaac aaaccatgcc tttcctgcct 48300
ccaagcccta cacctgctat ccatccctct gtctgagaga cactcccacc ccttcacaaa 48360
gcctgtttct catccttcca gttcagatgt cttctcagct tgcctcaact gacctctttc 48420
agctattctc actctttgta ctctgttcat ttccttcctg gcagtcacca taatttatct 48480
ttatttgaat caatttctta gttgtattat ttagttattt gcacactctg tctctctgtg 48540
cctttcttat tcactgcagg ctttcttatg taagtaattt atttacttaa atttttaaaa 48600
ataatttcaa cttttggccg ggcacagtgg ctcacgcctg taatcccagc actttgggag 48660
gccgaggtgg gtagatcagc tgaggtcagg agttcgagac cagcctggcc aacatggtga 48720
aatcccatct ctatttaaaa tacaaaaact agccgggcgt ggtggtatgc acctgtaatc 48780
ccagctactc gggaggttga gggaggagaa tcacttgaac cggggaggtg gaggttgcag 48840
tgagctgaga tcacgccatt gcactccagc ctggggcacg agagtgagac ttcatctcaa 48900
aaaaacaaaa aacaaaaaac ccctgctttt cagaggggct gaactaattt acattctcac 48960
caatagtgta taagcattcc cctttctcta cagcctcact agcatttact tttttaaaaa 49020
actttttaat aatagccatt ctgactggta tgagatggta tctccttgtg gttttcactt 49080
gcaattctct gatgattagt gatattgagc attgttttat gtttgttggc tgttcgtatg 49140
tcttcttttg agaagtgtct tttcatatat tctgcccatt ttttgaatgg agttgttttg 49200
tgcttgttga attaagttcc ttatagattc tagatattag acttttgttg gatgcatagt 49260
ttgtgaatat tttctcccat cctatagttc tgtttactct gttgatagtt cctgttttgt 49320
tatgttttgt ttttttgctg tacagaagct gtttaatcta attggtccca cttgtcaatt 49380
tttgtttttg ttgcaatggc ttttgaattt taataataaa ttctttccta aggctgatgc 49440
ccagaacagc attttctagg ttttcttcta ggattcttat agttcaaagt cttatattta 49500
agcttttaat ccacctcaag ttaattttta tatatagtga aatgcagggg tcctgtttca 49560
ttcttttgca tgtggccagc cagcaatccc agaaccattt attgaataag gaatcttttc 49620
ctcattgctt attttgtcaa ctttgtcaaa gatcggatga ctgtaggagt gtggcttttt 49680
ctgggttatc tactctgtta cattggtcta tgtgtctgtt tttgtatcag tatcatgctg 49740
tttttgttac tatggtctca taacatagtt taaagttgga taatgttatg cctctgcttt 49800
gctgtttttg cttaagattg ctttggctat tgaggctctt ttttcacttc atatgaattt 49860
tagaatagtt ttttctaatt ctttgaaaaa tgaccttggc agtttgatag gaatagcatt 49920
gaatctatag attgctttgg gcagtatgct attttaatga tattgattct tcctatccat 49980
gagcatggaa tatttttcca tttgtttgtg tcatctacta tttcctttag caatgttttt 50040
tagttttcct tgtagagatc ctcctaggta tttcattttt tatgtgacta ttttaaatgg 50100
gattgcattc ttcatgtggc tctcagcttg aatgttattg gtgtatagaa atgctacaga 50160
gttttgtaca ctgattctgt atcctgaaac cttactgaag tcatttatca gttctaggag 50220
cctttggcaa agtctgtagt gttttctagg tatagaatca tatcattagc aaagaaagat 50280
agtttgactt cttcttttcc tatttgaatg ccttttattt ctttcccttg tctgattgct 50340
cttccagtac tacgttgaat aggagtgctg agagtgagca tccttgtctt gttccacctc 50400
tcaggggaaa tggttccagc ttttgcccat tcaatatgat gttggccatg ggtttgtcac 50460
agatggctct tattattttg aggtgtattc ctttgatgcc tagtttgtca aaggccttta 50520
tcatgaaggg atgttggatt ttattgaaag ctttttctgg gtcttatttg gtgaattgca 50580
tttattgaat tgtgcatgtt gagccaaact tccatcccag ggattaaacc tacttaatca 50640
tggtgttaac tttttgatgt gctgctggat ttggtttgct aatttttttt ttttttttaa 50700
gatggagtct cgctctgtcg cgcaggctgg agtgcagtgg tgtgatcttg gctcactgca 50760
agctccacct cccgagttca tgccattctc ctgcctcagc ctcccgagta gctgggacta 50820
caggcacccg ctaccatacc cagctaattt ttgtattttt tagtagagac aggatttcac 50880
catgttagcc aggatggtct tgatctcctg acctcgtgat ctgcctgcct cagcctccca 50940
aagtggctag tattttttta attactattt tttctcaccc ttgctgccat cttatgattt 51000
tctagtattt tgttgaagat ttttgcatct attttcatca gggatattgg cctgtaattt 51060
tcttttttca tttcatcttt accacatttt tgtatcaggt tcatactggc ttcatagaat 51120
gagttcagga atggtccctc ctcctcgaat tttctctgta gaattagtac cagctctttg 51180
tgtgtctggg agaagttgta tgccaataat ttaaatgcag ttaatattta ctggacaatt 51240
tcctccagat aattgtatat gatttttggt ccaccctgag ttgatacatg tattttaatt 51300
gtatcatggt atgaaaagag caagagttat ttggtcacct agtcttgcct atagatgttg 51360
cctaatgatt caaagtagat attttgggag ccttaacagg tgccgtggac taggcagttt 51420
tgtttttttt tttttttgag ggacagagtc tcgttatgct gcgcagggct ggagtgcagg 51480
ggcatgatgt aggatcaatg caacatccgc ctcgtgggtt cagagcaatt atactgcatc 51540
agcctcccca gtagctggga ctacaggctc acgccaccac gcctggctaa tttttgtatt 51600
tttagtagag atggggtttc accatattgg ccaggctggt gttgaactcg tggcctcatg 51660
atccacccgc ctcggctccc aatgtgctgg gcttacaggc gtgagccacc gcacccggag 51720
attaggcaat tttatattcc caaatatcca actcttctga cccgctttct cagcctgggt 51780
gtatcaggca caaggcctgt tcagattatg tggtctctga agatatggct ctccagggtt 51840
gacaatgtgg ataaggattc acctggttta ggatttacac attcgccttg aatgtctgtt 51900
gcaccaagta gacagtccat cccaacttgg ccatttggtc agagctgtaa ggagacaagg 51960
aggtgggcag ccgctgctgt gaactgcttg gacaaagact gccaaatagc tatcagacag 52020
tgttaacaac agctgattta ggtttgaagg gggcagtctc ttgggccact tactatgctg 52080
catcatcctc tttggaaaat gctcttcagg taactgccta acagactgag aaaataaaat 52140
gctcacagag aaaaaagacc cggaaagtct gacttctcag agctcagtgt ttaggtgcag 52200
aactggattg tgaaaggatt tttaaatttt ttatattcat tgcagggaac attcatttat 52260
tccatccttc tccactccca cctgtctgtc gttgtctttg tctctgtctc cccacctctc 52320
tctctagaca cacacacgca cacacacaca cacacacaca cacacacaca cacacacaca 52380
cacacacaca cacacacaca cacacacccc tattcattgc caacagtaat agagttgctt 52440
ctttacttct tggagagaaa agcctcaatc tgaggaagct gtgctgacta gccttgctct 52500
taatcatgga gacaatgctt tatgccttta tctttgcaca gctgaaagcc atggcagaag 52560
cagtcctcta aacgaaataa aatagaaagg ttcctgctaa gccctggcaa atgcagcctt 52620
ctatccctcc cccaacactc acagcttctg agcaagatgt tgctgccttc caggagctgg 52680
gtgatgggca ataatgagca gagccacgtg aaggaaagat gggtgaagaa atgtgtgtgg 52740
agtcatgctg gctgcactga ccatgaaaca aaggatctac ccctctagta actgccctac 52800
tcctttggta actgttctga aattataact tgccagaagt tcagaaggac ctagtgcagg 52860
tattagagga aattcgtaag attgagccat ttattcctgc acagatacat aataatggac 52920
acgggccatg gtggccagca ttcttgctct tgacaatggt gaagggaagg gttgtaggtc 52980
atggctatgc tctcagaatt ataatggaaa gaaacagctc ctgagtgttt actatgagcc 53040
aagggctgtg ctaaacactt taccatatga tgacatcttt ttctcacagg tatcaaaaaa 53100
caataggaca taccggatag ctacaatctt tgggcccctg caaacacaat aatgtgtatt 53160
ctcttcttca aatcctacat attgctacaa actgtatccc tgaggcatat tcattgtaaa 53220
ataaaaacat ataaagtact acttttgttt tttgagatgg agtctcgctc tgtcacccag 53280
actggagtgc aatagcatga tcgtggctca ctgcaacccc ctgctcctgg gctcaagtga 53340
ttctcctgac tcagcctctc aagtagctgg gattacaggc gcacgccccc atgcctggct 53400
aatttttgta cttttaatag agaccaggtt tcaccatgtt ggccaggctg gtctcaaact 53460
cctgacctca agtgatccac ctgcctcggc cttccaaagt gctggcatta cagctgtgag 53520
ccactgcacc cggcccatat aaagtactac taatgtaaca gggtgctagt ccagacagtg 53580
accacacgtg gtgttcattg aaggctggac taacaactcc agcctctccg ccatcacaga 53640
gtgatgactg ccttccctga agcaaagctt ctggttcaag gaaaggccag taagtgactg 53700
ctctttgttg tatacatgtt agatgatcag gcctcaagaa aagtataaag agatctttgt 53760
gctctctggg actcaaaaag ctgcactctt tgggggaagg atagccaggt aaaagtggcc 53820
caggtaaaga gggcctggta cacctggttc tgcaagatgg tagacacaaa aatgagagct 53880
acatttggag cttatgtgcc cctaactctg tacataacct gcaagatcta attactaaca 53940
actggaatct tggaaacacc tgtagtacat ccttggctaa ggttagcccc aacagagagg 54000
gctctcctct tacagagaac cattacattt gtgccttcat cctagagtag aaaaggcatg 54060
atcagactac taaaaagaca tcaggaaagg gcctgtgaca tctgagggaa gtggttgccc 54120
tctctgggat gttggttcgg gaagaggggc atggaggagt gcctgcttta gatggtcatt 54180
caggaaccca ggctgatagt gagaggtgaa gccagttggg cttctgggct aggggggact 54240
tggagaactt ttgtgtctag ctaaaggatt gtaaatgcac caatcagcac tctgtaaaat 54300
ggaccaatca gcaggatgtg ggcagggcca aataagggaa taaaagctgg ccaccagagc 54360
cagcagtggc aaactgctca ggtccccttc cacgctgtgg aagctttgtt cttttgctct 54420
tcacaataaa tcttgctgct gctcactctt tgggtctgca ctatctttat gagctgtaac 54480
actcaccgtg agggtctgtg gcttcattcc tgaagtcagt gagaccacaa acccactggg 54540
aggaacaaac aactctggac acgccaactt taagagctgt aacattcact gcgaaggtct 54600
gcggcttcac ctctgaagtc agcgagacta tgaacccact ggaaggaaga aactccagac 54660
acatctgaac atctgaagga agaaactcca gacacaccat ctttaagagc tgtaacactc 54720
actgcaaggg tctgcggctt cattcttgaa gtcagcaaga ccaagaaccc actggaagga 54780
aacaattccg gacacatttt ggtgacccag atgggactat caccaagtgg tgagtaccat 54840
caaccccttt cacttgttat tctgtcctat ttttccttag aattcggggg ctaaatattg 54900
ggcacctgtc agccagttaa aagcgactag catggctgcc agacttaaga aactaaagac 54960
acgggtgtca gactttctgg gaaagggctc tctaataacc cccaactctt tggagttggg 55020
agcgttggtt tgcctggaac cagcttccac atttcctgta cttctgggct gagacgaggg 55080
tcaacataga ggaaagccat tcagctctgg ggtcccgaca gcaagttggt tgaccctgtg 55140
gccatgatca caactctcga agtcatgttg cccaagcgag actcacccat ctatcctatc 55200
tatcctgact cttgcttcct gggtcctaat gcctggaaga caaaacttcc tcttgtctct 55260
gttctccaag gctagtccca cttctaaaaa ccactccctg tctctggtgc ttttctagtt 55320
tctcctataa gaatgatttc tagtataaac tccaggactc tattctcttc tttaggcacc 55380
cgggctcacc aatcagaaag ccataatttt tgcccaaagc cccatcttag gggggactat 55440
ctggaatttt aggatccctc ctcagacaag caggcctaac aaaagctatt cctgaagcta 55500
ggatatgggg agcctcagaa atgatatcct tcctattcaa gtgaggacaa aaggcatcac 55560
tcttccaatt ctggagatcc cttccctccc tcagggtatg gccctccact tcacttttgg 55620
ggcataacgt ctttatagga cacgggtaaa gtcccaatac taacaggaga atgtttagga 55680
ctctaacagg ttttcaagaa tgtgtcggta agggccacta aatccgattt ttctcggtcc 55740
tctttgtggt ctaggaggac aggtaagggt gcaggttttc aataatgtgt tggtaagggc 55800
cactaaatct gacattcctt ggtcctcctt gtggtctagg aggaaaacta gtgtttctgc 55860
tgctgcatca gtgagcgcaa ctattccaat caacagggtc cagggaccat tgtgggttct 55920
tgggcaagag gtgtttctgc tgctgcattg gtgggctcaa ctattccaat cagcagggtc 55980
cagtgacctt tgcgggttct tgggtcgggg ggtgggggga acaaacagac caaaactggg 56040
ggcagttttg tctttcagat gggaaacact caggcaccaa caggctcacc cttgaaatgt 56100
atcctaagcc attgggacta atttgacccg caaaccctga aaaagagtgg ctcattttat 56160
tctgcactat ggcctggtcc caatattctc tctctgatgg ggaaaaatgg ccacctgaag 56220
gaagtataaa ttacaatact atcctgcagc ttgacctttt ctgtaagaag gaaagcaaat 56280
ggagtgaaat accttatgtc caaactttct tttcattaaa ggaaaatcca caactatgca 56340
aaacttacaa ttcacatccc acaagaagaa ctctcactta cccccatatc ctagcttccc 56400
tatagctccc cttcctatta atgataagcc tcctctatct ccccacccag aaggaaacaa 56460
gcaaagaaat ctccaaagga ccacaaaaac ccctgggcta tcggttatgt ccccttcaag 56520
ctgtagcggg ggaggggaat ttggcccaac ccaggtacat gtccccttct ccctctctga 56580
tttaaagcag atcaaggcag accaggggaa gctttcagat gatcctgata ggtatacaga 56640
tgtcctacag ggtctagggc aaaccttcaa tctcacttgg agagatgtca tgctattgtt 56700
agatcaaacc ctggccttta atttaaagaa tgtggcttta gccacagccc gagagtttgg 56760
agatacctgg tatcttagtc aagtaaatga tagaatgaca gctggggaaa gggacaaagt 56820
ctctcccggt cagcaagcca tccctagtgt ggatccccac tgggacctag actcagatca 56880
ttgggactgg agtcgcaaac atctgttgac ctgtgttcta gaaagactaa ggagaattag 56940
gaaagagcct atgaattatt caatgatgtc caccataact caggaaaagg aagaaagtct 57000
tgccttcctt gagtggctac aggagcctta agaaaataca ctcccctgtc acccaactca 57060
ctcaagggtt aattgattct aaaagatatg tttattactc aatcagctgc agatatcagg 57120
agaaagctcc caaaagcaag cccttggccc tgaacaaaat ttggaggcat tattaaacct 57180
ggcaaccttg gtgttctata ataggggcca agaggagcag gccaaaatgg aaaagcgaga 57240
taagagaaag gccacagcct tagtcatggc cctcagacaa acaaaccttg gtggttcaga 57300
gaggacagaa aatggagcag gccaatcacc cagtagggct tgttgtcagt gtggtttgca 57360
aggacagttt aaaaaagatt gtcctatgag aaacaagctg ccccctcacc catgtccact 57420
atcgctgaag caatcactgg aagccacact gccccaaagg acaaagatta tctgggccag 57480
aagcccccaa gcagatgatc caaccacagg actgaggtgc tcagggttag cgccagctca 57540
tgtcatcacc tcactgagcc ctgggtacat ttaaccattg agggccagga aattgacttc 57600
tactggacac tggtgcggct ttctcagtgt taacctcctg tcctggacag ctgtcctcaa 57660
ggtctgttac catccgagga atcctgggac agcctatatc caggtatttc tcccacctcc 57720
tcagttgtaa ctgggagact ttgctacaga tagtaagtat gcttacctaa tcctacatgc 57780
ccatgctgcg atatggaaag aaagggaatt cctaacttct gggtgaaccc ccattaaata 57840
tcacaaggaa actatggagt tattgcacac agtgcaaaaa cccaaggagg tggcggtctt 57900
acattgccga agccatcaaa aggggaagga gaggggagaa ctgcagcata agtggctggc 57960
agaggcaggg aaagacaagc agaaaggaaa gagagaaaga gcagaaagtg agagagaaag 58020
agagatagga agtgatagca aagagggagt cagaaagaaa agagagagga gagagagagg 58080
gggaaagaca gagagagaca gaggaagaga cagagagaca gaaagagaga agcaaagaga 58140
ggaagagaca aagaaggagt caaagagagg gaaagagaag tagtaaagaa aaaacagtgt 58200
accctattcc tttaaaagcc aggttaaatt taaaacctat aattgataat tgaaggcctt 58260
ttctgttaac cctataatac tcccaatacc accttgttgt tcagtgttaa acaagggtta 58320
ttagcccaaa agccactgag gccactgaca acccgtagcc ttcttatcca aaatccttaa 58380
cacagcaggt ttcctaacag ggatctaatc ttaggtcgac cagactggag aactgccttc 58440
aggacaggat gatagatggt tcctcccagg tgattaagga aaaagacaca atgggtattc 58500
agtaagtgat aaggaaactc ttatagaagc agagttagga aaattgcgaa ataagtggtc 58560
tgctcaaacg ttgaagctgt ttgctgtttg cactcagcta aaccttaaag tacttacaga 58620
atcaggaagg agccatctat accaattcta agttaatatg gactgaacga ggttttatta 58680
atagcaaaga aaattaaaat ctcaaactta cgaggttttc aagtaaagta aagtttggta 58740
aaagttaaca gcgtaacatg tattatccta gtaccacaca ttctctcaaa ggatttgctc 58800
agacagtttg caaaaaagaa cgaaatctgt ccttactcta caatcccaaa tagacttttg 58860
gcagcagtga ctctccaaaa ccgctgaggc ctagactctc atgttgagaa aggaagattc 58920
tgcacttctt aggggtagag tgttgttttt atactaacca gtcagggata gtatgagata 58980
ccacccagtg tttacaggaa aaggcttctg aaatcagaca atgcctttca aactcttata 59040
ccaacctctg gagttgggcg acatggcttc tcccctttct aggtcctgtg acagccatct 59100
tgctaatagt cgcatttggg ccctgtattt ttaacctctt ggtcaaattt gtttcctcta 59160
ggatcgaggc catcaagcta cagatgatct tacaaatgta accccaaatg agctcaacta 59220
acaacttctg ctgaggaccc ctggaccgac ccgctggccc tttcaatggc ctaaagagct 59280
cccctctgga ggacactacc actgcagggc cccttcttca cccctatcca gcaggaagta 59340
gctacagcgg tcatcgccaa atcccaacag cagctggggt gtcctgtttg gaggggggat 59400
tgagaggtga agccagctgg gcttctgggt caggtgggga cttggagaac ttttgtgtct 59460
agctaaagga ttgtaaatgc accaatcagc actctgtgtc tagctaaagg attgtaaatg 59520
caccaatcag cactctgtaa aatggaccaa tcagcaggat gtgggcgggg tcaaataagg 59580
gagtaaaaac tggccacccg agccagcagt ggcaacccac tcgggtcccc ttccacactg 59640
tggaagcttt gttcttttgc tcttcacaat aaatcttgct gctgctcatt ctttgtgtcc 59700
acactacctt tatgagctgt aacactcact gcgagggtct gtggcttcat tcctgaagtc 59760
aacagaccac gaacccactg gaaggaacaa agaactcccg atgtgctgcc tttaagagct 59820
gtaacactca ctgcgaagct ctgcagcttc actcctgaag tcagtgagac cacaaaccca 59880
ccagaaggaa gaaactctgg acacacctga atatctgaag gaacaaactc cagacacacc 59940
atctttcaga gctgtaacac tcaccgcaag ggtctgtggc ttcattcttg aagtcagcaa 60000
gaccaagaac ccaccggaag gaacaaattc cagacacagt aggaaatctg tatttttgat 60060
ctgtggcttc cagggttact ccagtcattg aagtctccat tgcagcctta aggaaacaga 60120
gaatggtttg gaggagcaca tgtgggaatt gttatggacc aggcttgaga tgcacatagg 60180
gcatttctga tcaaacctag ctggaagcag ggccaggaaa tataatctaa ggaagacagt 60240
ttttgtagac agtagtagtc tttgcatctg agacatgtag attatcaagc aattaattag 60300
aaaaaatata gccaggtgcg atggctcatg cctgtaatcc cagcactttg ggaggccaag 60360
gggtgtggat cacgaggtca ggcgttcgag accagcctgg ccaacatggt gaaaccccgt 60420
ctctactaaa aatacaaaaa ttagcctggt gtggtggcac gcatctgtaa tcccagtact 60480
caggaggctg aggcagggga atctcttgaa cttgggaggc agaggttgca gtgagccaag 60540
atcacaccac agcactccat cctgggtgac agagcgagac tctgtctcaa aaaaaaaaaa 60600
aaaaaaagga aaggaaaata taatcaagaa tattgacagg taacatttat tcaacactta 60660
ctatgcacca ggcaatacac taagtgtttt acatggatta actcatttaa tcttaacaat 60720
agccctatga agtcagtgct gttattatct ccactttata gataaggaaa ctgaagtaca 60780
gaaaggtcaa gtagagaaat ggccatgctt gcattctcag tttttgaagc aactgttaca 60840
ggaatctggt gtgagaaatg ctctaacaag atgtgagtca ggggttggga ggtactgagt 60900
ctgagttggg cagttgggga tggaaggatg gatgaagaac agcttgacag agaagctgac 60960
acttggcaac tctgtgggac cttgaagggt tagagggact tcaccaaaga aactggtggt 61020
cagggatacg ggagggtcac ggcaaggagg gaaaggaaac tgtaccacag cagagagtct 61080
gaagctacta cagtgtagtt cagcgtataa agaataatta ttttaaggta aacttataac 61140
ctcatgcaaa tataaaatga acacgtgtca aagatcttat ttaatttatt aattaatgag 61200
ggaacctgta agatgttaca gccagttcaa aggataattc aaataaatcc atgcacatat 61260
gtaggcaata aggaatgctg aaatgaattt aaaagtagat gtaaactgat ttatccacag 61320
agaaataatc agttgcattt cacataacaa aattcagttg cttttctaca gaaggaattg 61380
tttgcatcat taccaatttt tctacaacta acagaattat aaaataactc aaacacaatg 61440
aaaggcagat ataacccaca atggtatgat agatacaata tccacatcca ggatgttttt 61500
ttctcatttc aaagtctttc acaagttttc ctgataaggg agtgtcaata atactgtatg 61560
gcaggcaata agactggatg gatggttggg gccaggtttt aaggggtaat aaatgccatg 61620
taaaggtatg tgcatactgt gcaacatgtc ggggaatctc aaattattgg tagagtatgt 61680
aagaaacact tgtggagctt gttaataaat tcaaattccc agacccaact cctcaagggt 61740
ctaatacagt aggtttggag taaagcctga aaatctgcaa ttgtgcaaaa aaaaaaaccc 61800
aggtgattct gatacacttt gagaagcact ggtggaacta atagtcactg aacgtttttg 61860
agcaggggag aaacctgagg acgtctatgt tgcagcagtg gaaacttgat tagaagtagg 61920
agaagatgca tggtcttaaa agaatgcaaa atgatggcta atatttgagt gcttatgatg 61980
ggccaggggc tgtgctaggc gcgtggcaca cattcaatac gatggaagcc tgtaccagtc 62040
agtattagtg gggtatcttt aagagtgacc agaattaagg ggggttttca ccaaagcctg 62100
aggactgagc ctcctcatcc taaattcaga cacaatgctg tacctatgca tttgcctcca 62160
ggctgttcct gggcctccag ggactggccc aggctcctga taaataggga ctcccaacaa 62220
cataaagcct ggattttgga acttcctgaa tgttactcag gctttctagt aactgtggag 62280
atctgaataa taacacaatt ctaagttccc ctactcataa agctgctcat catttagatg 62340
gggtaaagca cctgaaatac aatgagcatc actattttca ttcatccatg aaatgaacat 62400
tccggggaga tcagtaagtt gatgtatcac ccttgaacag ggcaaaatga atactcacca 62460
ggaatatgtg gtattttaaa aagaaggcaa agggaagaat agtggggatg gggcaaaaac 62520
tttaaataga ttcccccaat catatatggc aattgaagat aattaaatta tcattttaat 62580
tgagtaagta ctcatagagc cctcactatt tgaaaatgaa ctgcctccta attgttattg 62640
tgcaaatgtg atacattaaa cttaagctat tttaataaaa catccatttt cggaagctgt 62700
agtaggttct cccaggtcag atttgataag ccataaagaa caaatgccaa ctcctatttt 62760
tctatggtgc tgggaaataa gagagaaatg tgtaattcaa agcaatcatt taattttatc 62820
caatagcttg attctcctct ctcttctagc cttttagcta agctgttacc aagtaaccac 62880
actagttggc ttgagtctta ccactgtttc cctgacccca cagtggagag actgcatctg 62940
ttaaagagca gttatgtaac catggctatg ctgagctggg attcccaagg cttaggttct 63000
ttctgtgaat gaccttcacc aagacacctg aggtctgtgt ggaaccacag gcttgtcatc 63060
tctaaggcag agttgataat tccatctgtt tcttgagccc acactgagaa aaagattaca 63120
tgactgcagt tatttgaatg cctcatggaa agacgtctta taaatattat aattaatgtt 63180
atcattaagt aatgcttcaa tgcagatctt ccaagtataa atatcagctg agtaagaagt 63240
caatcttccc tgaagcaaaa ttgaaatttg taaatgcgat ttctgggagc ttattttgta 63300
atacatgatt ccagagtgtc cataacacac acaattgtct tttttcccct acatgggcta 63360
tttacaacaa aattggactt ataatgttta tttccaggga tgactagaac tttaataaca 63420
aaccttgggc caggcatagt ggctcatgcc tataatcaca gcacttcggg aggctgaggc 63480
tggttagatt acttgaggcc aggagtttga gaacagcctg gccaacatgg caaaaccctg 63540
tctctactaa aaatacaaaa attagccggg tgtggtggcg catgccagta atcccagtta 63600
ctaggtaggc tgaggtacga caatcgctgg aacctgggag gcggaggttg cagtgagctg 63660
agattgcact actgcactcc agcctgggtg acagagaaag actctgtctc aaaaaaaaaa 63720
aaaaaataat aataataata ataaaccctg atgaaaggtt tctaaaatgt tttcatctaa 63780
tggttttctt gacaattaaa ttttctatat aatgtcagtt cataaaaaaa ctgagaacga 63840
ccacatgtca tatcgactgc ttaaaagaaa atacgtatat ttacaaacat atacacaata 63900
ctgtcttttg tctggttagt ttagaggtta gataaactgc agtatgttgt agtggacaga 63960
tcatagaact aggagtcagg atgtctggat tcctaggaag caatgaatag gttgcacggt 64020
gcagctcaag gttattcaaa gtgtggtgcc cagaccagca tcatgagtat cctcagggag 64080
cttgttagaa ctgcagatcc tttaactcat tgaatcagaa tccctaggtg tggggccctg 64140
aaatctgtat tttagcaggc tctctgggat tgtgatgtgc cttagagttt gacaaccact 64200
gggtagctga tcctgactta gacttatcag gcatgtgatc ttgaacaagt cacataatct 64260
cactgagttc agttttctta tgtttaaaat aggcccaata atatctattt cacatggatt 64320
gctttgagga ttaggcaaga gatctgtaac agacactgta gaacagtgtc tctggtctac 64380
agctgacctt ccataaatgg tagttgcctt gattctctgc tctgccacat aatagctggt 64440
taactatgag caagtaattt agttcttctc agtttagttt cttcccctgt aaaagaagga 64500
aaataactgt tatactccat ttctgaattg ctataaaagt catttaatta tgggcattga 64560
agctctttgt tcactgtata aggactgtac atctaaggga ttaatgagac caggcttatg 64620
attttaagca tggagtaaat agtaacactg actctgttct atgaaccaca tggaaactct 64680
aaagaatatg cacatttgaa acacaggtat catctgggga aggtgatctg ctcacccaaa 64740
ccagttcatg aacatcaatc tccagtggcg tgctggagct agctgtacca gctcatgagg 64800
gccaattgtt tcatttttag gaattttgtt tgctggttaa aaatagtcat tatttaaaat 64860
taaattatgt aaacaataat attagataaa ataagttaaa ataaaaacaa aggaactaat 64920
tatccccaaa ctcttcccca cctaattatt ttactatctg tgccttggga ttatttacat 64980
tgattttatc catatggtga caatactatt catatataaa tggtgtgctt ctcttcataa 65040
ctctacatag cctgatgtca ggctagtagc ttgaaattgg ccacagtggg agtgtgagca 65100
tttgtaccat gaggcttggc caaggctaca aatccagact tttgtttttc cctcctggag 65160
agctgtctgt taaaaattta ccaacacacc actggtctta cctttgttaa tttaccacag 65220
tccaggttct gacctagact tagaaacctg gatttgtcag caagctgagg atagagccat 65280
tatttctaag aaggactcac attacccaag tgcaaagcct gatatatacc ttcagaatat 65340
caatttatta atttacagtg aagaaagcca ccccagggca ttccccaggg gaaggcaaaa 65400
agagctagtt gcacattttg aatgtttgat gacattaggg taaggtgaca cagaatatcc 65460
atttccacaa ctgagatacc tgctgcctta aggaagggac aggcaagtcc ttgggcagga 65520
ccttagattg tcactgtcca tcttgctgta ggactctcct ttccaggcat gacgatggcc 65580
aactctgtcc tcctacccta ctgatgggat tatcttttct tgacacatgg caatgcctcc 65640
aatcagaggc tggtagctat ttttaatctt cagggcagta tttttcaaag ggaagttcat 65700
ggaccatatg catctgtatc atttagatgt atattaaaaa tgcttagtct tccccagtta 65760
tactagatca gaatctctgt tggtggggcc cacgaatcgg tattttcaac aaatcactag 65820
gtaatttctg tatatactat agtgtgaaga ccactgcttg aaggtttctt tgcatatctc 65880
cactaaatat aaaaaatatt gacttctaga tttaactccc aaagcacttg catttttaag 65940
tttctggggg cattatattg tggtacccct ataccactca cactctagtc aggaggtata 66000
ttatggactg aatgtttgtg tccctccaaa actcatatgt tgaagtctta gcttccaatg 66060
tgatagtatt aggagatggt gccttctgga ggtaaaatca agccctcatg aatgggatta 66120
gtgcctttag aaagagagct cgtcactgtc tttccatcaa ttgaagatgc agtgagaagc 66180
tggtagtctt gcatctggaa gagggccctc acacaacctg atcatgctgg cacctggtct 66240
cagactttct gcctccagaa ctatgagatg ataaatttct gttgttcata ccccacccag 66300
gctacaatat taggttgctg caaagtattt gtgatttttg cctttacttt tcagggcaaa 66360
aactgcaatt acttttgtgc caacctaata ttttgttata gcagcccgaa ctaaggcaag 66420
ggagactaca tcagacagtg tagctatgta agtacaaatg tatccctgtt gaaggaaaac 66480
taagttctaa ccctgacttc aggccagtag ccaccttttc aatctctttc atgaagggac 66540
cattatcatt atcactggtg gcaaaaatag agcacgagaa tggaatttgc ttttctgtga 66600
aatctcagtg tatacagatg aagagcaagg gtttgctttc atctctaaga agcaaaagtg 66660
agtacggact ggcacattat cagagaaaga atcattctag ctcggtgggt cttaaccagg 66720
agtgaatttg actccaggga acagttggca atgtctggag acgtttttat ttgttatagc 66780
tgggggatga gtgggtgggt tgctactggc atctagtggg tggagaccag agatgctgtt 66840
aaacatcccg caaagcacag gacagtcccc gacaacaaag aattatctgg ccccaaatat 66900
caatagtgcc aaagttgaga aacctcattc tagcttcctt ttcccttcta cgttctaatc 66960
aactgttgtt ctttcagcat taggattcat ccagcagtct ctttccccag caatttgttg 67020
aaattttttt aaaaatggac tcattttagt gtcacaagaa aaaaatacat tcacaggaaa 67080
ggatgggtca ttttgtttaa tgatgttttg cctttcacat agcaaaagct taataaagta 67140
tttttaaata aaatggtgaa tagatcaaaa cattaatttc acatgtgttt taataaataa 67200
caggaagatg gctatattat ataaattgtt cttgtatatg tcttgagtgg atcatcaaac 67260
acaaacgtat ctacatgcct tttcttgtga atagatctaa taataacgct cttctaaaaa 67320
caaattaaat ggatattatt tgctgagaat gtaatgcttg tgtgaataga agccagccct 67380
gaatccaagc ccccagatct atttaaagaa tttgaagaat gtcagaaaag cacgtggctt 67440
caaggttaat gtgtaagact cacagaaact tgaaaaatca ctatgactaa aaagaaagta 67500
tgagctccct gcatgcctgt aaattggaat gacagccaaa accagttaat tataaaaaca 67560
gctaatttaa caggttttca aatttgtttc tttctccaag tagcatatag tcaataatcc 67620
ttaaagagaa agcaaagaag gggaagcact gaaccaaatt tgcttttttg tacctgctca 67680
gctcaaatgc agagttctct acctggaaat tgactgcttc catagtttga tagccacaga 67740
gagatgggaa cagaaggaga ggtataatcc cagacttgat tcagctatag agaatgacaa 67800
tagtgtcaga ggccttccaa ccagagcgac tccatcttga atacgggctg ggtaaaacag 67860
ggctgagacc tactgggctg cattcccagg aggctaagca ttctaagtca caggatgaga 67920
caggaggtca gcacaagacc ttgctgataa aacaggttgt aataaagaag ccagccaaaa 67980
cccaccaaaa ccaagatggc catgagagtt atctgtggtt ggtctcactg ctcattgtat 68040
gctaattata atgtattagc atgttaaaag acactcccac cagtgctatg acagtttaca 68100
ggtacattgg caacttccgg aagttaccct ctatggtcta aaaaggggag gaaccctcac 68160
ctcccagaat tgcccacccc tttcctggaa aacttgtgaa taattcaccc ttgttcagca 68220
tataatcaag aagtaactgt aagtatcctt aggccagaag ctcaggccac tgctctgaat 68280
gtggaatagc cattctttta tcctttactt tcttaataaa cttgctttca ctttactgta 68340
tggacccctg tgaattcttt cttgcaagag atccaaaaac tctctcttgg ggtctggatc 68400
aggacctctt cccagtaaca atagtagtaa ggggtcgggg aaactggaca aaggagttta 68460
agaagcctta gataaagggt cctcatcatt gtcataacat aaaatcatgg actcctagaa 68520
ttttatagct gataggatta gaaatttcaa aattcaattt cattaatttt catctgcgaa 68580
aacagatggc cagagaggcc aaacaatttg ttaaggagca ctgaggcgat ggaacaccac 68640
actggaccgc aaacctccta gcagagtata caaggccttt gatctcctca gtcagaatga 68700
actagagctt tccaggggta ccctttctga ctgtttagca tgtttgccag tctgactaat 68760
tttgaagttg cttaaatatc tgtcatttcc actgtatcat aatctcctca ttcatcttca 68820
atctccaatg ccttgaactc agtaaatgtt agttgaacaa aagtaaattg aacccagaat 68880
ttctgatcat aatctggagc actttaaaat tgtcagctta ctgggaaacg ggataacatg 68940
tgatttgtct ttgatttttt ttttctcata tgctttttcc acctatagat gctacacgaa 69000
tgtttttaaa atctgatata aaaattaaaa ttaaaaaatt aaaaaaagaa aatttgatac 69060
aatgctacat ttagagtgtt gtgattagat tccttaagtg tatcatggtg atctctacat 69120
cacgtggtga tcaaattgct ttgggtttta acacataact gacaaaggct tggggacatg 69180
taagatccca aatacatttt tattgatttt tttttcttgt ttgtcctctt ttaaataact 69240
tttttttgtt ataagaataa ttcatgttca gtggagaaac catagaaaat agtgacaagt 69300
gaaggaataa atttaaaatg acccataatt gtaccataca ttctgatttt ttaaacgctg 69360
aacaaattag ccttgggtaa gtaccaggaa tagagtgcag cattgaaagt taaagtttgg 69420
ggaaggatag ctgacttaag aaattatcta gttagacatt ttttggatgg ggtaattttg 69480
cagatgacat tagtgagaga aaggacttgc cactctcaca cagctagtag gggtgtggga 69540
ggatattgga accaagtttc aagtcttcag tgaagaatca agggagaagt tctaaaacct 69600
aacaatatcc ctctggatgg acatttattt tattactaca ataagccaca cggtgagtca 69660
taaggagcat ttcattcttc taatatgtct ctactgtatt tagaatctga taaagcccta 69720
ttagaattca tctctttaag aataaaagaa gctgaggaac taaagagagg gttggaataa 69780
tccactaatt atatccgtta agcttcagtt acgctaataa ggaatatcac atgactgtgg 69840
tgtgtgcttg ttctgaacag taaagtacat gaggaaagat aagattcagg gctgaaatgt 69900
ccttcagcat atgtaggtag tggtgatgaa agtcattaaa agaaaaattg attgaggtat 69960
tttagtaaac aaaagaactc accacttacc catcaggaag tgtattgtta atgcagtgct 70020
gttcagcctt ctggaagaaa aggtttcttc atgcttctct ctttagccta attcttatcc 70080
tgtcactttt caggcaaaat taaaaaaaaa aaaagattga aaacgatgct cctattttat 70140
ttgcttcaaa agaaacaggc tgttgcattg tgcttggaac agtttactct tggccttgat 70200
gtaagtgtga aaggaagccc atgtaattga ctaggcagta tctgaagaag caggaaatac 70260
agtgttaaga aaatgaacag gcatgaaaac catggctatt tgataaaagt aaataatttc 70320
tgcagttcac atgttctcag catattttct ttgatactga cttgcttaat atgacaatag 70380
cagaaccatg gtagcttgta ggcattactt ttcttttaat ttcttttaca ttttgaattt 70440
accagcactc acatttgtat tacttttggg ttatactgag gatctataac ttatagatca 70500
aatacctgac atatatatgc attctctgaa gtcttagggc agaactagaa cattcttgtg 70560
aacatcagta taagatatta aaatggaagt tttgcctaag actgaagaca ataaaaatat 70620
catagtctga aatgaatgcc agcacaccat acaggattta aatatctata catatatatg 70680
tgtgtgtatt atatatattt aatatatatc tgtgtgggat aggaagaggt agggggaaat 70740
cagttttaca attattaagt atttcaccct tgacaagagt atatatattg gaaatcagtt 70800
ggagagtatt ttcaaagata aatgttagtg tgctatgaat gaatccaccc ctaccaccac 70860
tgaggcaggg taggagaggc ctgtgctcct caagcatagt tggaaaagga cctcaacaag 70920
accacttcaa gagtctaatg tgtggagact gttgcttagg gagaccttat ggtctagctt 70980
ctgactcaca gctaagtcag ggagacaggt tggctgctct gatcgtggag tccaaaagat 71040
ggcctgcact gaaaagcctc atgagtgttg acttagggct agtctaagag gtccctggaa 71100
gaagaaacac tcagtaggag agaagctgga ggtaccttca gtgctgaatt ggaactagat 71160
tcattccccc gtggagcaaa ttacatagga aagatgccca gtgatggaga gtgggggtgt 71220
ctctaacaat tacccaccca ctgcccccac cctaagaaaa agaaaatcac atacaaccag 71280
tcagctgtaa acatatgccg agcctagtaa actcagatac taagttacca gggtacctgg 71340
caagtaagaa cattcctgat tcccttcctc tcttctcttt gccctccaac cttagtggct 71400
agcaagatgg ggagaggagg agaagctgta agtggggaaa aaagagcagc tttctctcct 71460
tttcagctgc tggattctcc ctcatcatag gcctgagctg gggaatcagg aagaaggatt 71520
ctttttaaaa ctgaagtaac gttatcattt aattttaaaa cattttaaat tttgacaatg 71580
ttgagattag atatactaat tattaaacta agattatgtt ttgcagcttg aagtgataag 71640
aaaaactctt atctaagagc atccaggaaa gtcgggggtt tcctgaacat ccttttaaat 71700
cctttggaag tcagctttca gagaggattt aaagtgtaga ctgggccttc agaaacttgg 71760
ttaatgtagg ggtttcctat gcagacttgg ggactatacc ttgtgtggaa gagagaaaat 71820
aagattatct tacatttttc ccattccttt ttcaaaaaga aagctcagct agcatgaaag 71880
ttaaattcaa aacgtaatgg gtattatttg catattcaaa tctagtgcat atcatgtaag 71940
tactgaatta tggtattcat tatttcaaat gacaagctgg attttttttt ctttcgaatt 72000
tcacaaatta attttccttg gaaccttttg gtttgggctt taagagttta ggctttcatc 72060
acaaagagag gacagccttg aagattaaag tgtgtggctc ttctcaagat gttcttagtc 72120
cagcaaagga ttctatgcat atttgggctt ccttctgtct cataacctgt atttcttgat 72180
attctattta tattctgtaa gatttttttt ttaaaggaaa aattcttcca tggttgaagg 72240
acatgtcaaa aatagaggat acagttttat atcaaaggaa gtttcatgat atgactgtag 72300
aagctcattt gacttaagac acatcatttc ctcatggaag tgttaaacag atctgtacaa 72360
taaggttggc aatctttgtg taaaacagtt ttttttctcc tgctctaaag aaagtgtata 72420
tttcaaaatg tgaatgtcag cagtcagaaa atagtatttt tttaacttcg ttttcaaagt 72480
cctcaaaaac ctgtacctaa tcatgaattt tttttcccac agattgtttc ttcttctccc 72540
tcccagaaac tttgaagttt ttctacatga caccaggacc tatgtctttt tttaattaca 72600
cagaaatgaa agaaaaaaag tgtgttgtat cgttaaccaa atatatgaaa tctttaagct 72660
gtatttttat ttttaacttt gttttgcaaa gaggccattc cctttggtta aataatttgt 72720
tattcacagt ttccttgtcc tcatattatc aaggggaaaa ttgtagaaat tttaaaggaa 72780
gctctaggca atgttttcat ccctgaatct ttggagagtt ataaaaacaa acagattact 72840
gaacctgtaa gagaaccaat cgtgaagtca ttacatctaa gcataagcaa aatctcctct 72900
tggatcatta agttatagaa gaaaagaaag cctgcacttt gaaatttaga taaagcttgg 72960
taacttgtaa gtcaaacacg taaaatttta caattcagga atatcgatag cagttgagtt 73020
taatagactt ctcacattcc aaatttaaag cttccttctc tgtgctaata gagatacaat 73080
agcagtaggc gtttaagaag aatgaatcaa caatttaaaa ctataatgtg ttttttattc 73140
atctccctta ttcacatata tttgttttgt tttgagaagg agttctgctc tgtcgcccag 73200
gcaggagtgc tgtggcacga tctcagctca ccgcaacctc tgcctcccgg gttcaagcga 73260
ttctcttgcc tcagcctcct gagtagctgc gattacaggc gtgcgccagc aaccccggct 73320
aatttttgta tttttagtag agacagggtt tcaccacgtt ggacatcttg gtctcgaacc 73380
cctgatctca agtgatcagc ccgcctcggc ctcccaaagt gctgggatta caggcgtgag 73440
ccatcacttc tggcccttat tcgcatacaa tttaaaaatc atcacagaag gtttgaaaga 73500
aggaaggggc agaaaattac ctacttttcc tctccccagc gatctccttc aaatctgtgc 73560
cttttcctca ggcccaggcc tcaatttact gagcagtcac acctcacaga gggaggtctg 73620
ggcaatccac tcttggtcac aggaaagcca ttgaccctcc cacttcctct cctccacctt 73680
gttctcaact cttgactttg ggctttgttt ctgttcaagt cctagaactg gtttctttta 73740
tcaggttaag tgattagttc tctttccctc tagttgctct cactccctga ctcttgcctt 73800
ctgtaacaac tggagacaac tctttcaaaa ccagctccaa gccccagact tctctctggg 73860
ctttagttcg taaggcaggt gccctactga gtgagcctag atcagacaga aacatagctg 73920
ttggcaagga tttaggtgaa tttccttcca ttgtttttct aatacctttt tttttttttt 73980
gtaaatataa ccatgcacct acacacatat ttgaatatcc tgccttttta tttaaaatga 74040
catgataggt ccgggagtgg tggctcatgc ctgtaatccc agcactttgg gaggccgagg 74100
tgggcagatc acctgaggtc aggagttcga gaccagcctg gccaacatgg tgaaactcca 74160
tctctactaa aaatcaaaaa ttagccgggc atggtggcag gctcccagct actcaggagg 74220
ctgagatgtg aaaatcgctt gaacccggga ggtagaggtt gcagtgagct gagatcttgc 74280
cattgcattc cagcctgggc aataagagcg aaactccatc tcaaaaaaaa aaaaaaaaaa 74340
aagacaggat aaacattcta gatagtctct ataatggtca tgattaagac aataaaatag 74400
tctgaaattg tcaatatata ttaataataa tttatttggc cattctgcca agtagcagac 74460
acctgtcatt ctgcccactc agcacctctc tttcttttag ggaaatgcta cccactcttt 74520
gcatgggttc tggatggaac tgttgatcac agtgttttca ctccccattt tgcctcacca 74580
gaggtagaca gaagacccaa gccaggccag ttacacacaa tcttcagata attaccgtat 74640
tgatcacagt atcaccccac tcaaggcttg gttggagatg agcagaagag actaaagctg 74700
ggtcatttta attaacacct gtaccccaaa gaaagactgt caatgaggct tttataccga 74760
cactcctggt ttccattctt cctgatgcca ttcatttgac gaactaccca atctttccaa 74820
cagtgtcttt ggaagaaaga tagtcagaaa agaagataga gttgttttct gttctttgca 74880
accaaggaac tctaaatgat agacttgttg ctaggcactt tggttatttt tattatcttg 74940
aatacttctg tgatatactt ctttgtgcat gcctgtttgt acggatgtag ctttttatat 75000
attttatata atttctcaga agtggaatta cttagtcaaa aggtatgaac attttctgat 75060
tcttaatata aattgtgcaa atgcttttta agaagattat accagtttac attttgtgtt 75120
atatataaca gaaagtacta ctgaaaaata ttacaaaaat tgtctctctg ttcaggagga 75180
cttgtaatag atgataaagt acttgaaata ggaacataga gcattttcag tttaaaataa 75240
tttcattggg ttatttacgg aatccttaga attatggcca gacatttata gatgatctgt 75300
accaaaccta gttggttaca taaattgctt attcaactgg cttaaatcta taatagaaag 75360
atgacactta ctgaatgttt aatatacact ttgtcagggg ctttgtatta ttctatgaca 75420
tcttcaaaat gaccctactt tcctatttta taagtaagga caggaaggct tcaagaacat 75480
gactaatttt cccaagggct gtaccaaagc cagaacccaa atctataagg cttttaaacc 75540
tgcattctaa aactgcatct cggccatctt attcctacag aacttaaggt tagaaagcca 75600
gattggagtc ccaatttcac cacttagtaa ccagacaaac ttgaggaatt cactcaacgt 75660
ctttgaatct ccatttccta atctttaaaa ctaaaacaat aatactggcc ctacctattt 75720
cctaaaattt cgtgaggcac atagagctag tgtggtagag tgctgtacag atgtcaagtg 75780
ttagcgtgaa ttacttagat ccctgaacac catggatgaa tgtgtctgac tgctattaga 75840
ggtcataaag aatattgggg ccaggtacat tggcttattc ctataatgcc agcactttgg 75900
gagcctgaga caggaggatc actcgaggcc acaatttcaa gaccggcctg ggcaacatag 75960
tgagacccct tctctacaaa aaaaaaaaag cagccacgtg tagtggcaca cacctgtagt 76020
cccacatact caggagggtg atttgggagg ataactttag tccaggagtt tcaaggtgca 76080
gtgagctgtg attgcaccac tgtactctaa cctggacagc agagtgagac cctgtctcta 76140
aaaaaaaaga aaaaaaaaat aataataata aagaataatg ggccttggga tacccactcc 76200
tctctttctg ctctgagttg tgaagcagtt gagttacata tgcatgtcca atggatgagg 76260
ttgaaaatat caactggatt ggaatgtggc ttacttgcgt ggccacaatg agcttcgtaa 76320
cacttcctga cagggtgaga agacaaactt cctcacccag tcactggcag agctggacac 76380
tctgtgtctc tcccacagaa caacctctta ctgcatggag gtggatgaaa aagtcaaccg 76440
agaacaggct actccaaaaa gcagagcacc aaaggcacca gctggtcagg tcccccttcc 76500
taagtaaaca atcacgtaat tcattcggga caaagccaga gaggtggtgt ggagaaagag 76560
agggcagttt cctcccaagt ttttcctgga attctttatg ggaatatgag gtttagggga 76620
ataagacttc cctttaacag tgaagaatcc ccagctctat tggtaatagg aaatcgctta 76680
caaggatcat ggggagtatt tcctcagctc gttctgcctc ctacttggct gagtggaatg 76740
gaaccatctg tggctgctgc atatgatatt gtcaactttg tcattccaca cccactcctt 76800
gacgccctac catgtggtca taagactccc tttaaagtgt tcctttaaaa aacaaaatgt 76860
gttttgtttc tataaaatac agctcaatgt cagaaccctt gtcttgtttg ctctctgatg 76920
taaccctttc acaatgtttg ggcagcttat tctctctatt tccctgtagg gtcccatcca 76980
ggccaaagtg agtgccagcc tcatttgggc agcacatgcc ctgtggaagg gcaggaagag 77040
acgaaagcta attgtaactt tgtgattagc tgtcatggat gcctggtcct gtcaatagcg 77100
ctcaataaag ccagaaggcc aagcgttcgc ttctgcatac tgattgctga gtcagatttc 77160
tcagtgcaga agggctttct aggcagtcaa ttttagaata ttagtcttgg ttcttaagtg 77220
gttaaaatcc ctagctggtc tttaatctga gcctggagaa tttagttagg gctgacattc 77280
tgctgtgata tttttgccct caatatatat gtctttcctc catctcttag atccctgaat 77340
catagagata tatatgttat ataatcaact gtctccagtc tctaagagtg ataagtacac 77400
attgtgtcag gttgagggga caggagaact ttcaaaagcc tttcttgccc ctttttcctt 77460
ctcactgcct cccactaagt ccagccactt attattcagc tgacactatc atcatgacca 77520
tgagtctttt ggggctaccc tggttcggat ccttttggag gtttgttgct taactctgtc 77580
ttcagtccta tggagctgct ttttcaataa gtttctattt tggctaaagt tggccagaat 77640
ctccttgtaa ccaaagaaca aataaaatac cagcttgcaa tgttctatgt tgcttccacc 77700
aaacttatgc agcacttcct atctaatcca cctactagtc tttttttttt ttattttttt 77760
ggagacggag tctcgctctg ttgctcagga tggagtgcaa tggtgcaatc tcggctcact 77820
gcaacctctg cctcccgggt tcaagcaatt ccccggcctc agcctcctga gtagctggga 77880
ctacaggtgc atgccaccac gtccggctaa tttttgtatt ttaggagaga gagggtttca 77940
ccatgttgcc caggctggtc acgaactcct gagctcaggc aatccgccct cctcgggctc 78000
ccaaagtgct gggattacag gagtgagcca cctcacctgg ccccgaccta ctagtcttta 78060
gtgtttgctt ccttctattg ggtaattgtc tgtttatatg catgtcttgt ttcctcaaat 78120
aaaatgtggt cttctcaagg gtattggccc atgttctatc catctgtaga tatcacagca 78180
cctagcagtg tctttcacag aggaagtaca caactggcat tattgattca ttgctccatt 78240
ttttccttct ttatccccag catttctcaa taatttcaaa catctccatt ggagtaccgg 78300
agaaagcagg tagctttact tgcagctatg tttctatccc catagtaact aaaagaggac 78360
ccagagaaac atgtttaaat gctgtcctgt tatcaggacc tcagccttct gatgctccgt 78420
ggcttggggg ttaatgcttg atcatttcct ccccaaccta cactgtgtac ctatgctagt 78480
ctcttcatga ggactaagcc ccatagtaaa agggctagat aaatagaaaa tcattttatg 78540
taattataag aatgagaata ctgagtatta ctggtgtttg tttaggataa gcacatcttt 78600
atttgtatga gaaaaagaaa aagagagtga aaaatatatt aacgtgcata tagttcagga 78660
ccatggattg caagtgacag aaactcaatt caaaccaacg taagtcaaaa ggaaaatata 78720
ttggctcatg taaccttctc acagagaggg caggatggaa ggggctttgg gaacaagaga 78780
attgttctca aattctagga atactaggat tagtccagga tgggtcacct tcctgtccct 78840
gaggtggtgg tagcgatggt agagtcttat gggaggaaag agtgcatgtt aggatgaagg 78900
tagggctaag caaacaaggg caagggccac tatatcatgc taaaaatggt tttttttgat 78960
gtcttcctta atttcacaaa tgcttccaac aaagtagcac acaggaaaaa gaacataggg 79020
actctactgg tgggtgcttt tatcttaagc cttgtacttg cttttcacag cttactcact 79080
gcttgtacct gaggccatat gccctgtaaa agcttctgca gggtttctac taagctgggt 79140
tccttatatg gctctctccc atttctgttg cctcactcta gtgatctttc tcttttcctc 79200
acctctggga ctggtggctg tttgtatgga ctgccttagc tttgctttgg gttttttcct 79260
ggggacaatg tcttcagatt atcctagacc aaataaacta cagccactgg gccaggctct 79320
tcctcctcca actggaccat gttcccaggg ctcttcacct tagtttaggt caagcattct 79380
tggcaaaaga aaggcctagt taacaataga cattctagca attgattctt tttgacatgt 79440
tgtaagatct attcacattt tgtaattaaa gcattcccct atggaaacca acacgaacta 79500
agctgctcct ggaatgcagg gtggcctcct caatacagga tgttctagag agctgtattt 79560
tgggcactta actattctcc actacttagg gcacagcact gaaattaaca ccactaagtt 79620
tgtcatgtcc atgtagttag tctcaggcag tgcagcctca ggagtggaac tgacctctta 79680
tgtgtgtcca gcctttcttc cttcagaagt cagctgtgtt ttctgctgac tctccatagg 79740
aacatcagtc ctgaatcctc agaccaccat ctggagtagt aagtgctcct gacagtccta 79800
gaagttgtct accgctggat ctccaaagcg tgtgacacac cgtgagagag aaatgagaaa 79860
gctgggctct tcaggtaaat cttgcttttt cacaagcccc ctaattttac tgcataatta 79920
ttttgaattc actgataatt tctacaattt tcccataagt catctacaca caataccctc 79980
tcatgcaaca cttggctttg ctaatacata tctattatga gagctgtgct tcttaagcgt 80040
aaatgtttta tatgcactaa ggctcttggc ttacatataa aaggggtatt gagcaatgtg 80100
atacagaagt cttttctcca caggtctcat atgtaaagaa ttcattagat tggctgaaat 80160
agactgatct gtccatttct ctgctcactt atcataagga agtcattagc taaggaacaa 80220
aaactacaat ctatgtaatt agaagaacaa gctggttttg ctcaatataa aaataagaaa 80280
aagaaaccat gtgaaagtca aaatatttgt ttaatcaggt cattgagaat ctattaaaaa 80340
gtatttgaat tctttatgat gagaactatc ttgactcaag tggacagtgg tgagcttttt 80400
ggcctgtggt ccctacgtag aaaggaggct ttgtcataaa gtcttatatg gtacaggtgc 80460
caagttaagt gcccaagctt gctcttaaaa gcatactgga ttttgtttta gacttttagt 80520
gaactgaagg gaataaacaa atccctctgg gagaacttct cctccatcct tggtgaagtc 80580
attctgccag aattc 80595

4

80246

DNA

Nucleotide sequence of NC-contig

4
tggttgattt gtnnataagg aagtttggaa tcaatcccgg aaggaatttt ttttttaaaa 60
aattttttgg aagggtttgg tawtaaaaaa rccaatttgg gtttttaaaa ataggaattt 120
tatgggaaaa aattttccct tttttttttt ttaagtttta gatgttatgt ttccttatac 180
ttaaagtggg tgtcttatag gcagcatata tctgggtctt gatgtattat ttaatctgat 240
aatctcaacc tttttgttgg agtgtttagg ccatttacat ttagtgtaat tatagacatg 300
gtttgatttg ctataccatc ttttcatttg ttttatatgt gagccatctt ttcattgttc 360
ttttttcatc tttgaccatt ttctttagta ctgaatactt tttttgtatt tcattatatc 420
tattggcttt ttagttatac ctcttaaaat ttttttttct gttttatgta ggatttataa 480
tatacatctt taacttatca cagattacct tcaaatagta ttttaccagc tcaagtgtaa 540
tgtagaaacc ttacaagagt atattttcat ttctgtctcc taatttttat gctattgtct 600
ataatacatt aggtttgttg ttgtttgttt ttaccttatt gctgttggct ggggtcagca 660
aacattttct gtaaagggct agatagtaca ggcatacctt ggagatactg tgggtttggt 720
tccataccac cacaataata caaatatgca agaagtggat atcacaataa agtgagtcac 780
acaagtcttt tggcttccca gtgcatataa aagttttgct tatactacac tgtagtctgt 840
tcagtgtgca atagtgttat gtctaaaaaa acacatacct taattttaaa atgctttatt 900
actaaaaaat gctaacaatc atttgagcat tcagtgagtt gtaatctttt tgctggtgga 960
aggtcttttc ttattgatga ctgatcgggg gtcaggtgct gaagcttagg gtggctgtgg 1020
cagtttctta aaacaacagt gaagattgca atatcagttg actcttcctt tcatgaaaga 1080
tttctctcta gtgtgtgatg ctttttgata gcattttatg cacagtagaa cttctttgaa 1140
aattggagtc aatcctctca aaccctgctc tgctttaaca acctaagtta atataatatt 1200
ctgaatccat tgttgtcatt tcaacaattt tcacagtgtc ttcaccagga gtagattcca 1260
tctcatttcc tgagatggaa tctttgctca tccataagaa gaaattcctc atctgttcaa 1320
gttttatcat gagattgcag caatacagtc atgtcttcag gcctcacttc acttttaatt 1380
ccagttctct tgctgtttct accacatctg tggttccttc ctccattgaa gtcttgaacc 1440
tctccaagtc atccatgagg gctggaatcg acttcttcca aattcctgtt aatatttata 1500
ttttgacctc ccatgaatca tgaatgttct taatggcacc tggaatggtg aatcctttcc 1560
aaaaggtttt caatttactt agtccagatc catccatcca gaggatccac tttcaatgcc 1620
agttatagcc ttatggaatg tatttcttca ataataaggc ttgaaagttg aaattactcc 1680
ttgatccatt ttctgcaaaa tagatgttgt gttagcaggc atgaaagcaa cattaatctt 1740
tttgtacatg tccatcagag ctcttgggtg accaggtata ttgccagtga gcagtaatac 1800
tttgaaagga attatttttc ttagcagtag gtctcaacaa tgggcttaaa atatttggtc 1860
caccattctg taaactgatg tgctgtcatc taaactttgt agtttcattt atagagcaca 1920
ggcagagtag atgtagcata attcttaagg gacttaggat tttcagaatg gtaaatgaac 1980
attggcatca atttaaatca ctagctgtat tagcccccaa caagagagtc agcctatttt 2040
ttgaagcttt gaagccaagc gtcgacttct cctccctggt tacaaaagtc ctaaatggca 2100
tcttcttcca atataaggct gttttatcta cattgaaaat ctgttgttta gtgtagccac 2160
cttcatcaat gatactatct aaatctcttg gataacttgt gcagcttcta catcagcatt 2220
tgctacttca ccttgtactc ttatgtaatg gagtggcatc tttcctcgta cctcatgaac 2280
caacctctgc tagcttccaa cttttcttct gtagtttcct cgcctctctc agccttcata 2340
gacttgagga tagttagaga cttgctttgg attagatttt ggcttcagga aatgttgtgg 2400
ctggtttgat cttctatcca gaccactaaa actttatcca tatcagcaat aaggctgttt 2460
tgctttctta ttatttgtgt gttcactgga gtagcacttt taatttgctt caagatatat 2520
ttctttgcat tcacaacttg gctgactggt gcaagaggcc tagctttcag actatcttgg 2580
cttttgacat gccttcctca ctaagcttaa tcatttctag cttttgattt aaaatgagag 2640
atgtaggcca ggcacagtgg caggcacagt ggcatatgcc tgtaattcca acacattaag 2700
aggccaaggt gggaggattg cttgaaccca ggaggtggag gttgtagaga tcacaccact 2760
gcattccgtc ctggatgaca gagcaagacc ctttctcaaa ataaaatgag aggtgtgctt 2820
cttctttttg tttgagccca tagaagccat agtatgattt ttaattggcc taatttcaat 2880
actgttgtgt ctcagagaat agggaggtct gaagagaggg agagaggtgg gggaatggct 2940
ggtcagtgga gcagtcagaa cacacataac actaataaat tgtttgctgt cttatatgga 3000
tgtggtttgt gatgccccca aacaattaca atagttacag caaatatcac tgatcacaga 3060
tcaccataac agatataaga atcatggaaa agtttgaaat attttgagaa ttagcaaagt 3120
gtgacacaga gaaacaaagt gagcacatgc tgttggaaaa aattggtgtt gatagacttg 3180
ctccatgtaa gtttgccata cgccttcaat ttataaaaaa cacaatatct aggaagttca 3240
ataaagtgaa gtgcaataag atgaagtatg cctgtaaata tttcaggctt tccagaccat 3300
agggtttctg ttgcaactgc tcacctctgc cattatagca tgaaagcagc tatagaaaat 3360
atacataaat gaggcctgta atcccaacac tttgggagcc caaggtggat ggatcacttg 3420
aggtcaggaa ttcgagacca gcttggccaa catggcaaaa ccccgtctct actaaaaata 3480
caaaaatgag ccaggactac gcatgcctgt agtcccagct acttgggagg ctgaggcagg 3540
agaatctctt gaacccggga aggggaggtt acagtgagcc aagattgtgc cactgcactc 3600
cagcctgggc aacagagtga gactgtctca caaaaaaaaa aaaaggaaaa gaaaatacac 3660
ataaatgaat gtatgtggct gtgtaccagt atatcctcat gctctagctt gccaaccctt 3720
gctttacact gtcagttacc ttctaaagag attaaaaatc ataacaatat ctattacgtt 3780
tattcacatc ctagtgtcat ttcttcctta tgtagaatca aatttcattc tggtatcata 3840
tttcttcttt ctaaataatt tcctttaata ttttttatag cacaggtcta atagcaatgc 3900
attatgcaat tcattgctat tagacctgtg ctataaaata gcaatgaatt atgtcagttt 3960
ttatttgtct gaaaaagttt tttgtttttg aaatatactt ttgctgggta tataaatcca 4020
tgttgcataa cttctctttt cttcagcact ttaatgaagt cactcagtta tcttctggct 4080
tgtatagttt ctctggctgc cttcaagatt ttttcattgt ctttaatttt tagcagtttg 4140
atgtgtctag gagtgatttt ctttgtattt atccttttgg gggcctctta atttctttga 4200
tccttttttt cttttttttt ttttttaaac cattttgggt ctttcccccc atttggggtg 4260
aaaaaaaaaa aaaaataaaa tcatagttta aaaaactaat tttggaaaat tttcagctat 4320
catttcttca aatatttatc ctactctatg ctcccctcct cccctttcct tctgtgactc 4380
aaattacagg tatatttaac cattttattt gttcacggca cttggatgct ctgctttctt 4440
attttttgtc tttcattttg gataatttct actgacctat cttcaagttc actgattctt 4500
ttctcagtca tgtctagtgt gctcaacgcc tgttgaagaa atcctttgtc tttaatatca 4560
tgttttttat ttctagcatt ttcatgtaac tctttgttct ggtttccatc tctctactca 4620
cttttttttt tttttttttt tttttttttt tttttttaga cagagtctcg ctctgtcacc 4680
caggctggag tgtagtggcg cgatctcggc tcactgcaac ttccgtcccc tgggttcaag 4740
tgattctcct gcctcatcct cccgaatagt tggaattaca ggtgcccacc accgtggctg 4800
gctaattttt gtattttttt agtggaaaca gggtttcacc atgttggcca ggctggtctt 4860
gaattcctga cctcaggtga tccacctgcc tcagcctccc caattgctga aattactggc 4920
atgaggcact gcacccagct ctgctgacat tttttatctt ttgctgcatt ttgtctacct 4980
tttccatgaa atcctttaac atagtagtca taattacttt caattccttg tctgacagtt 5040
ctgacattca agtctaggtc tgttaatact ttgtgaatct gttaacagct ttttttcatt 5100
cttgtctgtg tgttttgtat ttcttgattg tatgccaaat attgcctgta aaataaactt 5160
agataagtca tacttctatc cagaaatagc acattttttg tgtccagtca ttatgtggag 5220
gagttggggc agtctatcag tggctgaact agtttggatt tgttgatgct atacttagaa 5280
tgcaccagac ttccattcac tgcaagagtg ggctgctgcg ctttgtgatt catgtgaggc 5340
ctgaattgtg gaagggtttt tccttagtgt gtccctccat gctcagattt cagcaagtct 5400
tcatatctgt gccacagaag gaatctgacc catgctcttt ttgacctccc caagtgatca 5460
actgttgctt gttatagctt gtcatggagt aagagggtgt ttttttagtt ttcatcctcc 5520
agccttggtc ttgggccctg agctcctaga ctccaggagt ggatggaatc cagtgatttc 5580
tcagtaattc agccccttct ccagtagtgg cagatctctg ctttgtatca gtgcaagatc 5640
ctgggctgag ctcattttct gcccttcctc gagtggcaga cagctcttgc tttcaccctt 5700
ctaccaaagg cagtgcatct tttcttgggc ctctccccat tgaacttatg actttcacat 5760
aagagaaggg ctcatgtatc agagaattct gtgactttgt gccacataca gagtctctca 5820
gttctcttgc cctgccccag tcttttttgt gagcacctag tagagaccct tggagaagag 5880
caaggaagcg agtatggact tcttttgtgt ctgtcgattg ctttgtttct caactgctac 5940
tcttggactt taagaattca ttaaaatttc agctgttttc tttttttctt tcgtttttct 6000
tttttttttt tttttttttt agatggagtc ttgctctgtt gcccaggctg gagtgcagtg 6060
gtgtgatctt ggcttgctgc aacctccgcc tcccgggttc aagcgattct cctgcctcag 6120
cctcccaagt agttgggatt acaggtgccc accaccacac ctggctaatt tttgtatttt 6180
tagtagacac agggtttcac cattttggtc aggcttgtct caaactcctg acctcatgat 6240
ctgcccgcct cagcctccca aagtgctggg attacaggca tgagccaccg cgccaggcct 6300
cagctgttct ctttttacct gctgggatgg ctagttttct gtgtcaactt gactgggcca 6360
tgggatgtcc agatatgtaa ttaaacagta tttctgggtg tttctgtgag ggtgtcttca 6420
gaagagattt gcatttgaat tggtgaacta agtaaagcag agggccctgt ctagtagggg 6480
taggcatcat ccagtctgtt gaggacttga atagaacaaa aggcagggga aggttggaat 6540
tgccccctct ctgcttgagc tgagacatct atcctgccct tggcactcct ggttctcagg 6600
ggttcagacc tggattcctg gtctccacct tgcccatggc agactgtggg acttctcagc 6660
ctcctatcta attaataaat ttttttttac acacacacac acacacacac acacacacac 6720
acacacacac acacacccta tgtatccttc tgtttttctg cagaaccata tttaatacac 6780
ctgcttttat gacgattacc tatcgattct gtattctgcc aaaactgaaa acagttcatt 6840
tttccatctc ttctcagaga ggcttgtcag ccattagttc tctgatgggc tcaagaagtt 6900
atgcagtttt ttttttctca ctgttaggat ggaattgata ttctgttgaa actttctata 6960
cctaagtgga aacttgtttt gaggttattt tctctactta cttttgctgg aaatggaaca 7020
ctctgtatct agttaagaca cataaactga cttgtgatac cataatgttg tgttgaattt 7080
tatattctta gaaaatcatc tgtcaaggtg ttaactaatg gcaaagcatt taataaatca 7140
gcattcatgt attcaggtgc tctgaattat ctgactttta aattcttact ttataaatga 7200
gaaaattggg gcatggaaaa gttaactctc ctaaccccga attattacat tattaaggac 7260
aggacttaga ggccagatat cttaagtcat taatattctt tggctcacag aattggcagt 7320
ataacctaaa ggtaataact aggtgatttt cttttatatc aattaaatat gtcagttttc 7380
aaatattcat aagtacctac tgtgcaggga aagaacatgc catacaaaag atgtagtcca 7440
ggcctttaag aaactttcat ttaatgggaa ctcaagaagt gtacatataa ggagggaagt 7500
agcagtatgg tacaagataa tacatacata tcagtgaatg atattgccaa aaagtgctat 7560
tgatagagca ataattcatt tctgcaaaca gctgctgatc tcctactgaa aacagaggag 7620
ggagaacagg acgcctcgtg gtcaggatag aagagaaaga ccttgagttg agccttgaac 7680
agtatttaat attcaaaagg ttaagagagg agagcaattg aggaggggag aatagttcca 7740
gcacaaatga tggtgtacaa gatgaacaca gtcagtaaag agcagactgg tctggatgga 7800
gaggaggatt tgcatcattt gggattacgt catttagacc cttgaaagcc aggattgagt 7860
aaagccacag tgaagcgact ggctcgtatg gaagctttat tttaagaaga ttaatctggt 7920
agtgacatgt gccaaaaact gaataggtag aaatgagatg cagagagccc agttagaact 7980
aagtctggtg cagtaatgca ggattgaggc aataaacacc aaactacagt atcaccagat 8040
aatggatgtt tgaacggacg gtttaaagga aaattgatgg tatttggtaa tttattagat 8100
aatccagggc catggaatga gaggggaaaa tgactaacca tagtcatcaa atggtttttc 8160
ttaatgaatc tgaattttgg tgtaagagca acattttctt aggccttgcc tagttggtac 8220
agctgactat gataatgact gctaccatgc ttgttcctct tttagcagct gtgagtcccc 8280
caccagccaa acaatgagcc tcttgaaaag gacgatgcct tttcacttct ctccaagtgc 8340
ttggcaaata ggaggccttt tgaagttact ttatagttag gggttcccag tgagtatttg 8400
aaatattaag tcatgcccgt ggttgacagc atggccctac tgctcatcat cagctattaa 8460
ccttaggcaa gttaatgaac ttttctaagc cccagtctac tcatttataa agtgggatta 8520
ttaataatgt ctacttcata aaattatgaa gcctgagtta ggtcattcag atagtgttta 8580
gtctgattct tcgaacctag taaacagtca gtaaacagaa gcaaatgcca catgcctgat 8640
ttatatccaa ggggagaaag gtaaaagtga aattttcatg atttatggat tcaaattata 8700
catttcaaag atgctttata agctattgtt ttggtaagaa gaattgagct gaaacagaat 8760
tttctgacag cagtgattat taaatggtga aataggctat tgatgtcttt agaggatata 8820
gatgttcacc ttttgcatat aagtgcacaa aaattcacta agtagatatg tctgtctaca 8880
cagagagaga gagcgtgaga gcattaaagt tagtaaacat ccccctcgct tttttttttt 8940
tgagacaggg tcttactctg ttgcctaggc tggagtgcag tggtgcaatc gtggctcact 9000
gcagtctcaa catcctgggc tcaagcgatc ctctcgctca gcctcctgag tagctgaggt 9060
gtgcaccacc acacccggct aatttttaaa tttttttatt gtaaaggtga ggtttcacca 9120
tgttgcccag gtctcaaact cctgagctca agcaatctgc tcacttcagc ctccaaaaat 9180
gctgggatta caggcgtgag ccaccacgcc tggccagtaa accccattca tttacatcat 9240
cttacttgtc cctccaaaat cctgcaaagt aggtaggttc tgtctttatt tgttatttag 9300
gtgaagaact tgaagtggtg ttgaggaata ggtgttttgc caagagtcac gcagctggag 9360
tggcagagct gtatactctt ctgattccac caacgctgtt tacatcacat ctggagaaaa 9420
gtgctctgag gcacagatgt ttagtgggag ggatgagaca caggctgcaa tgcctaaaga 9480
taatcgggaa taaaagcaga aaacaagacg tttgtttctg ttaaaatgag acagaaaata 9540
aggcgtttgt tgtttgggat tgagcacttg gagaagtggg gagcgatttg atttgggtga 9600
gactgctcct ggaatgctgc atctggttct ggactactca ttactaggct tatagaaact 9660
agctggagga ggttcaaaga aaagctccaa aatgattagc gggctgacgg gattgattta 9720
taagaaatat taaaagaatt aaatgtgtat agctcagcta agcaaagatg aaagagacca 9780
gctaaatgta tacaaatatc tgaaacgtgc aaactttaaa aagagagatt aattatttaa 9840
catgatacac gggggcacaa tatgcagtca caggatgaaa atttcagctg agtatctaga 9900
agaattcccc gatagtgaat ctgttaaggc tgtctgtagt gtggcctttc cctggagagg 9960
caatagaaat ttcaagtctt acgattttaa aagtttcttg ggaactaggt attagatgat 10020
gttagagaat tattattaat ttggtcaggt atgataatgg tattgtagtt ctataagaaa 10080
aattgtattt tttagagtta cataccctga aatataagca tagaatatga tgtaggagat 10140
ttgctttaaa ataccacagt aaggaaagaa aggaaggagg aagaaaagaa aggaagggga 10200
agaaagggaa aaagaggcaa agaaggaaga gaaggtaaga gaaagaaaaa gaatgaagga 10260
agaaggctgg gcactgtggc tcatgcctat aatcccagca tttaggaggc caagttggga 10320
ggatcactta attaagccca ggagttcaag gctgcagtga gctgtgattg cgccactgca 10380
ctccagcctg ggtggcagag tgaagccctg tctctaaaaa aaaaaaataa gttaaaaaga 10440
aagaaaagga tagatgaagt atggcaagat gttggtaatg ttgaacctga aggaagttaa 10500
tatgtgagtt cactttcctc ttcagtcttc tttatgtatg tttgccaact ttcataataa 10560
acaatttaaa ttatattttc ctgatcaaaa cttagtagca gtattaatcc ctgggcttcc 10620
tgactagaac agcctcatta ccacatgggc agagttctgg ccgaccaggg accacgtagt 10680
ggttcaccat cttgctctgg taatgtggtc tgggctgaag ggccctttct aaggttgtag 10740
atagaaatcc aggaaacttg ttagaactgc agacctatca gggtacctgc aggaggtgag 10800
tctactaagg tgaaaaagca gagggcagag gtcgtgatta gcagctgacc gccccctgct 10860
tttctgtccc tcattcgtgg aaaattgagt ggagctcaat tttgagtgga gctctaagta 10920
gctccacttg tagacattga gtggagctct aagtgtcttc agaatagcaa aacactagtt 10980
ttctttttct tttctttttt tttttttggg agacagagtc ttggtctgtc ccccaggctg 11040
gagtgcaatg gcacgatctc cgctcactga actctgcctc ccgggttcaa gcgactctcc 11100
tgcctcagcc tcccgagtag ctgggattac aggtgcccac caccacgccc agctaatttt 11160
cctattttta gtagagatga ggtttcaccg tgttggccag gctggtctca aactcctggc 11220
ctcaagtgat ccgcctgcct tggcctccca aagtcctggg attacaggtg tgagccacca 11280
cacccagctg caaaacccta tttttcttga atggagaaac actttcccct tatttattga 11340
gtttgggaag caagaagagg ggtaattcat taagtgaaaa tttccaaaat ccagaaaaca 11400
tcgataaagc agcagcttaa tttttttaag gaagaatttt ttaaactatc ttcttttgag 11460
cctctttagg aagacctcac gtccttgcct tgaatgttga gagtgggaaa tccagggagg 11520
ttttggaatg catgccttat gtctgctttt ttgtttgtta gagaaatata aatattttat 11580
ctaggttttg ctgatggcag tcaagcatga acacaaccca ctgtttgaga agctgtaatt 11640
tctgaatttc tgcagagtgc acatctaggc cagcaaatgg cagtaagagt gaggtggatt 11700
tagctcagtg taaggatgaa ctccagaacc atcggctctg actgaaagtg aagcggcagc 11760
cgcgttgtgg gaaagctggc tggagtctct ctcataagca ggcattcttt ttctccagcc 11820
cgtcactgtg ttggtttggg cccacggtaa gcctcctggc ctctaggctg taacccccac 11880
catcctcctc tgcctcgcct ccagagtgat tgttctgaag cacaactgga tgtcattccc 11940
cttcctgaac tcctagcacc tacagggact ccatcccttg tgccccacat acctcacacg 12000
tagacattcc taatgaagat ttgattgaat tattgtaaac tcagtgcctc ccactcttct 12060
agttgcctct ctgcctgcct ttgtacattt atttatttat ttatttattt atttatttat 12120
gagacagagt cttactgtat cacccaggct ggagtttagt ggcaccatct cagctcactg 12180
caacctctac ctcccagact caagcaatcc tcccacctca gcctcccgag gagctgggac 12240
cataggcacg tgccactatg cccggttaat ttattgtaat ttttgtagag atggggtttc 12300
atcgtgttgc ccaggctagt cttgaactcc tggactcagg cgattcgccc gtctcagtct 12360
cccaaagtgc tgggattata ggcgtgagcc accatgccca gccgctagca ctcatcttaa 12420
tcgtatattt acttatctgg ctttcccacc agactgcggg ctcttcaaga gtaaatgcca 12480
tgttttcacc tttatttccc cagtttgtgg cacattctag gcactcgcca tcatgaaata 12540
aacctctgga gctgtgatat tacaaacgtg aaaagatgac gagcactcag caactttcag 12600
tgagtaaaca aaggctttca ttcagcatgt atttattgac tgccctgatc tgggctgctt 12660
cctgtctgtg gttcaaggag agcatagtct acagaaccag agacctggct actctggaag 12720
ttagacttaa gcccaccccg gtccttgaat ggggaaatat ttcccttcat tcctgtgttt 12780
tagggacaga aagatgagta atgcagtgat acatgctgga aatgtttatt ccactacccg 12840
aagctgcctc tcaacttaac aatccatgaa agaaacaaga tggtatataa ctttttctaa 12900
tttgtgatgc ctttgtttat ttgtttccgg ttaaaagagg aggtggcatt gaattgtttg 12960
tttggtttgg tttcttcttc aataagaagc atcttaatat aactagactg gacatctgtc 13020
ccattttcaa aaattacaag tttcgatcat tgctaaattg tacagatccc aatctgtctg 13080
ctctgcatac atttgcattt ataaaagcag aagcagacta gcagtctttc taatgcaatc 13140
ccccaaatgc atgaagtatt agattgcttc tccctattgg ttcatgcatt gctaaaggct 13200
taaaaggatc attgatttta attatttaat gtgtacagca ggctgagctt cctttctttt 13260
ttaagggaag aaccttcagg ggcattgctt tagtttttta atgttaaatc tcatttttct 13320
ttgaaaataa gaagttaaag ctgtattcac acaagctctc aaagtgccag attttcattg 13380
tgtttttaaa ccatctagga aatgtttgat tctaatgaaa cattactgct gaaaattggg 13440
ctgaaattgc tgggctgaaa atattgttat aacttcacat gattccagtg ttgtattatt 13500
attttttctt ttcctttttt tgacccgata tagatgaagc gaagagacaa gggagcaatc 13560
ccatgtgtaa taaaaaaagg cagcctgaat tgttgttgct gtttttgaaa tttaagctgg 13620
ttttcaatta aattcagtaa atggtccagg actataaatg ttgaacattt tttaccgtgt 13680
gatttaaaat ttagttttaa tgtttttttt ttgggttttt ttttttttga tggtttacat 13740
tttccccatg gaaagcagct atgtcatgtc ggcatgattc atcatggtaa catctcgggt 13800
tattttggtt tgtgttatgt tcagaaagcg gaatgccaaa aataaagagt ggtttgtgat 13860
gtctagtgtg tcttccttta acaaatcaaa ggcttttatt taatccactt aatgggacac 13920
tgcagaaatt taaaaaatgg aagtcccatc cacagaaggc aggtactatg atgtaaaaag 13980
tttaggtggg ggattaatag agtgatcata taatttatga gctaaaccgg aggcactttt 14040
ttttttgaga tcgagtctca ctgttgccta ggctggagtg cagtgacgtg atcacagctc 14100
actgcaacct ccgcctcccg ggttcaagcg attctcatgc ctcagcctcc tgagtagctg 14160
ggactatagg cgcccaccac catgcccagc taatttttgt gttttttgta gagatggggt 14220
ttcaccatgt tggccaggct tgtctcaaac tcctgacctc aggtgatccg cccacctcga 14280
cctcctaaac tgctgggatt acaggcgtaa gccaccatgc ctggcccaga gacacttttg 14340
agagtgaaga ggaagctgag aataattcac tgatctacaa ctgggaccat ccagggcaag 14400
ccagatgcca ttaccactag ctagaaagct tgccaaggtc tcatttacct tggtatatag 14460
caaattcttc tttgaattct ggaaattctg gtaagtcatt gaggtagctc tgtgccaagg 14520
agcaatatgg tagaattcta atatttcagg cagtacaaca ctttcctgca tttgtagcag 14580
gtaaagggag gtcagggcag aagacaaaac cactgggact cgacaaaggg cataaacgtc 14640
taatgcacct gatgtagctg atggtaaatt gttatcagct aaagatcttt cataataaat 14700
aaacttatca tttgtaggag ggcacagaaa tcgtggaaag ctgggattca ggttgcctgt 14760
ggctttaatt ctggaatcag aaatattagt caaggatatc agtctatgaa gtaagttttc 14820
aatgttatat gccacaagat gcagctgtcc tattttcact tccagtaatt ccttctgaat 14880
taatacacct taaaaatagc tgcagcttct caaatctgtg agaatcgtat gtgctgcttg 14940
ctacactttc ctttttcctg aaggcctctt tgaggtcttt caagaactca attcaattca 15000
gcaacaatta gggggtctaa ggtatacaga cgctgtgcaa gatgctcctg agacacaaag 15060
aggaggtcaa gcccctgcct tcaggcacct ctctataata taggaggaga aagagaagaa 15120
acactaatac acataggtag gtgccattaa aagggtgcat acattaaagc caggtggtag 15180
gtgcaagaag atttgtaacg tgagaatttt ctgcatgttt gaaatatctt ataattttta 15240
aaaattaaaa tgggagatac atatatatgt atttatgtat gtatatatgt atgtacatat 15300
acacacatat atacataaat atatacataa atatgtatat atgtgtatat agacataaat 15360
atgtatatat gtgtatatat acataaatat gtatatatgt gtatatagac ataaatatgt 15420
atatatgtgt atatagacat aaatatgtat atatgtgtat atagacataa atatgtatat 15480
gtgtatatag acataaatat gtatatatgt gtatatagac ataaatatgt atatatgtgt 15540
atatagacat aaatatgtat atatgtgtat atagacataa atatgtatat atgtgtatat 15600
agacataaat atgtatatat gtgtatatag acataaatat gtatatatgt gtatatagac 15660
ataaatatgt atatatgtgt atatagacat aaatatgtat atatgtgtat atagacataa 15720
atatgtatat gtgtgtatat agacataaat atgtatatat gtgtgtatat agacataaat 15780
atgtatatat gtgtgtatat aataatgtgt gtacatatac acacatatat acatacataa 15840
acattctgca ttataccatt cactttgtaa cccatcttcc ctaaaaactg tctcataaag 15900
agtcttcttt tccctgtacc tatgcaatgg taagtagcaa aacacacatt cttttgggtc 15960
cccataacat tccctgtagt ttgcccttaa cagtctttga tgtgaaattt actgtttctg 16020
tcttaacctt gcctgtctcg cgtacatgga gttttggctc ctggctccta gtctgcatct 16080
tcaccccatc ccttgcccaa agaatctggt tatgtgacca ctgctcatct tttctgctgt 16140
cacaactcca gtccaagcca caaacctctc tctcctggac tcctgcgggg agttcctttc 16200
tctccctgca tgagtctatt ctccgcacaa ctggcagagg taagtgagac tgcggaagag 16260
gcaagtttgc aagtccagag gaaatgaaga ctctgcttgt gcacatgctg ggtttgacgg 16320
gtgctggata tccgatggat ggcccttaag gtgagctcaa ggcttaaggg agagataggg 16380
gctgatgatc tgagattcat cagtgtgtgg ctgatgttta aacccagggg acaggataag 16440
aaggttattc cagggagagc gtagataaag aagctaaatg gcttctgggt ccttagtcat 16500
tcaaaatcgg acctctgagg caggaggaaa gcccagaaag agtagattcc tgggactcac 16560
gggataaaga ctttcaaaaa gtgggggctg gccagtgctg ctgaaggaag tagcaggacc 16620
ggaacagaag ggtaatcgtt ggacctggag aacttgaatt tgaattttaa ggttggtaac 16680
cttaaaaaag agcaatttta gatacctttt gaaattattt gcaagatttg tttggtatat 16740
gtgttattcc aggcaaaggg accagaaaag taaaaaatac ttactgaaca gttactgcat 16800
gcctggcact gtaacaccct gtttaattct cacggcaacc ctatagagta ggtgtcatca 16860
tccccatctt acagatgagg atatgaggtg cagctagatt aagcagtttg cctcaggtta 16920
caccaactgg ttaacgtaga gctaggattt gaacccggat gggctgatcc cagagctcat 16980
gctttaaatc gctagactgg tgctcacaga agactgggac cgaaaaaaat taataaaaaa 17040
aataaggagc cccctgggct agcaaattag gagttgttca gacagatgtg aaaaggaaag 17100
caaggcagag ggaaagtcac tgtacagaag agagagaccc atgacagcag agacagtgag 17160
ctggtaaagt ggctggcgat ctagcccctg aaaatacctc cagagaggca ggctcacgcc 17220
tgtaatccca gcactttggg aggccgaggt gggcagatca cctgaggtca ggagtttgag 17280
accagcctgg ccaatggcga aatcccgtct ctactaaaaa tacaaaaatt agccgagcat 17340
ggtgacaggc acctgtaatc ccagctgttc agttggctga gtcaggagaa tagcctggat 17400
ccgggaagtg gaggttgtag taagccaaga ttgcgccact gcatgccagc ctgggcgaca 17460
gagcaagact tttcttaaaa caaacaaaca aaaaagaaaa aagaaaagga aagaagaaag 17520
agacaaagaa agaaagagag aaggaaagaa aggaaggaag gaagagaagg aaggaaggaa 17580
agaaagaaaa ggaaagaaag aaaaagaaag aagaaagaaa ggaaagaaaa gaaagaaaaa 17640
gaaagaaaga aaatacctcc agagagccag gtctcttagg ccttctgaga aactcacatc 17700
ccttttgatg aacacaaatg cttcacactc tcaatgttat tggtaatcca agttatcaat 17760
atacctaaat cacttagtac tgaatctggc atatagtaat cacctaatga agagataaga 17820
gtcatggagt attctgaagc aattagaatc aatagactca atatacacat ggcaacaaag 17880
ttggatctta aaaaccgacc tgagtgaaaa aggaaaggga aagatacata acacggtacc 17940
attatgtaaa ttgataatat atgcttacac aatttgtaag aacacataca aatagataca 18000
tgtatattaa acatactcga acggttaccc tatggggtgg tggctggagt gggggtaagt 18060
ccgtaagctg taatggaacc taaacaaata catgaaacga gtaggaatca gaaggagtaa 18120
caataaaaat gtgccatgaa ctgaggagtg taaattaatc aactcactgc atctgaggtt 18180
aaaaatagaa agatgataat tgttattctt attactccta ggtcttccac ttgcactcag 18240
ctttacaatg ttggactatc cttcagatgg caccctcctt gcacttgctc aggcaggaga 18300
gctttttcct ccagctttct aggtgattta atatatcagg gaataagtat aaaaaaaggc 18360
acggtgctcc ctgggtagcc tttctggact tcagagctaa attgcaaagt cagttttaca 18420
catgtgattt catctatgaa attagggcaa ggtataaaac tggcacagaa aaaatgtgat 18480
ttattatggt gttactatcc cttacaagcg gagtgtcagc tgcctctttt tgtccactga 18540
tttaaggcaa gatgaactga aagtggctat gatcacgtct tcaaaagcac actctggccc 18600
ctcggctgca ggcgccctgc acattcccca gctgcgtgtc cggtggtgac acagtgcata 18660
attgtggcgc cttcctggtg caaactgtct cacttagctc cgtcttgctg gcacagcaga 18720
aaggaagaaa tcgaaaatgt ttggatttca aaggtaacaa gaagctggaa aacaactact 18780
ggccgagtct gagagtttca gcggagactg gtgcagcctt gtgtttttcc actgacagct 18840
gaaaatgagc ccagcttcag tgaagcttgt ttccttccct cctcaaggtt acccacaatt 18900
ctcagttctc tcaggaaagc caaaaaatga atttgagggt ttaggattgt ggttctttta 18960
tctattacag gattgataat atgttcctcc accagatgtt ctgcttgtaa caatactcac 19020
ttcctgacac tactgcatat gcaggagtgt cactaccaag gtaaacacag aattggctgc 19080
ccaattccaa atccctgaac tgagtgagag aaatcagaat tataataggg gattcaacag 19140
agctggctac ggatgtgcca gtggtcagat actttgctca tcatacgcag gtgctgctgc 19200
tctagcaact gctcactgct tcatttcctg ccttggtctt taaatactgc ttttctcagc 19260
tcaattggct ttcttccctc tggcagtcac gtttctttgg gtcaaacagc aaatgattct 19320
ttagaatcac ctggtactca aaggagctac aagacattgg gcatccactt ccactctctt 19380
ggaaaaacaa ttttatggaa gccaaggttg ccatagtgcc tcttgaggtt gtttgctcag 19440
ccaaggccca agctttgtgc ttcaaacatg aaattagaga gcttcagaac aagatccaca 19500
ttttcaatgg cctcacccaa ctggataaaa gaacaattgc catatctcaa tgaccacctt 19560
ttctcaggtg ggatggtaga tgctggaatg ggtcacagca ttgcccaacc aaactttgca 19620
aaaaaggctg gaagctctga ctggggaccc taaatatgca aaagttaata ggctcttcat 19680
tcagaatatg aaccccgtgt atggatatag ctaaagggtt ggcctttatg tttctattcc 19740
ttcacaaacc tggtagaata gatatgcttg tttcccttta aaaaatgtca acaattgcat 19800
ttatgatgct gtgtatagta actcacagat catgctccat gaaaatgctt cagaacccaa 19860
tataaggaga ttttttagcc atgtgtgaca aaagagaggc catttcagtg ttgaaattgt 19920
tcagagaagt atttgattat gttttctcag atctttttat ttttattttt tttgaaacag 19980
tgtctcactt tgtcacccag gctggagtac agtggctgtg gtctcggctc actgcaacct 20040
ttgcctccca ggttcaagcg attctcctgt cagcttcccg aatagctggg attacaggcg 20100
tatgcaccac catgcctaat ttttgtattt ttagtagaga cagagtttcg ccatgttgac 20160
taggcttgcc ttgaactcct gacttcaggt gatccaccca cctcagcctc ccaaagcact 20220
tggattacag gcatgagcca ccgtgcccag cctgttttct cagatcctgt attttgtttc 20280
tgaagccttc atttctatct tcttattcat tttggaagta gtacacctaa gtaaggtttt 20340
taacaatcaa atatctttgg aaaattccct ggttcctttc ttattcctac aaaaatatgt 20400
tcagtatagc tgatgttatg tttctttcaa attattcatt tctctatctc agaatttatc 20460
tcatgcctaa ttgttattga atagtcttca cttcttgtca tccagtttct ggtctcttat 20520
ttcactctaa gtctaattgg ctattagaat aaagagcttg taacagattc tttctccaat 20580
atgtcttatc ttttgactgc atgccagtga caaactgtta actgttttga ttcttcataa 20640
cattccacag aacatgctga ctcctctctt cctgaaagca atgcccaagc acagcattgt 20700
tagatagtat gtacgcaaca gggacatggg tgcatagcaa aaactagaag gaaggaggac 20760
cttccttagc aatgggtgat atggtccctg gacttagact ccaaagggtc gtgaggtgaa 20820
acacacatcg tccataccca ggaagcacac aggtgggatg gaagagctgt gcctaatgaa 20880
acttcatcca cgtggaggtg gaggaggctg cagctgcaag aactcagagc tgccttaccc 20940
agaccaggga ccagggaggg ctttctggag gaaacagcct ctgaactgcc agctgataga 21000
ggagctctac ctcaactctt ctggttcccc agggctgctt ttccacgtcc atttattggc 21060
actgaagttt gaataccttc aggggcccga aagcctgcca ggtcctcttc tctgcagagc 21120
aatcacacca acctgcaaag ggctaggaaa gggctgtcat catctcctac tcagaaactg 21180
gttcactgga aggactcagg ggccactgaa tacatcctgg cagctttcac aagaagggct 21240
tctgactcaa ggatgtttcc atctttgcca ggtcgccttt tctccttctc ttagagtttg 21300
gaggacgcaa atgtgctgag aagtcaacct ttcctgcaag gtgagacaca agggcctttc 21360
ccagcagaaa gaagagagca aatggaaggt ccttcttcct ccagtagagg atggactctg 21420
tctggcagcc acccaacagg aaaagcacaa tgcatgcctg cctgcttccc tccctccctc 21480
cgtttctccc tccctccctc cttcctccct tccattctct tcccttcccc tcccttccct 21540
tcccctccct tcccttcccc tcccctcccc ttcccttctc cctctccttc ccttcctctt 21600
cccttccttc ctcttccctt cctttcccct ccccttcctt tcccttcctc cctcccttcc 21660
tcccttcttt ccttcccttc tttccttcct catttcctcc cttccttcct tccttccttc 21720
ctttcttcct actttcctac ctttagggct ctgtgtcttt ggagtccatt ctgattatgc 21780
tgtaatgtct gccccttcct cttctctgtc aaaaaatgaa agacatggaa gccacttgcc 21840
ttttactgaa ttaaaaatta gtaaaagagc taaaaattaa tggttaaaaa tgtacgcata 21900
aattatgcag tatactaacc aatgaaaaga tacacttctc ttaattaaaa gctgacaggg 21960
agggaaacaa gaaaagagaa acacaaaaca ataatctaaa tgacctatta gttggaagaa 22020
caacatcaga gaaaatagat actgtgtata gtcatgtgta tgtctatgga ataacatttg 22080
tagagaaatc tggactgatc ctttctgagt aaagagagct gtgggtacaa ttaaggggag 22140
attgaaagga atccaaaagc atagcagatg ctgtgcctca ctggaatggt tgccgatctc 22200
ctccaaacta tgaagtgttt gaggctcaac tttaatataa ttaagataca aagacagaat 22260
gagagaaaga gagaagggag ctcactggaa gaacactcaa gattccttac tactcattct 22320
ctaaaattac aattgttcta gatggaaaag aaaaaaagct tctctgttaa aaaaggagct 22380
tgtgctatag gaggtttaaa atatacttct gacccatctc caacattcta aatccttccc 22440
agaaaagtat gccaatccca agaaatattc aatcaaattg ctggaaagaa aaatacaaaa 22500
tattaaaatg tattaggaag cgacagtaat taaatcagaa ctggagcagg aatagaccag 22560
cagatcaatg agacagacat caagtcccgg aatgtggact tgcaaatgca ttaagtaata 22620
tgatatgcaa taaaggtggc acagtgaacc aatgggaaaa aaattaatct tataataatt 22680
gatattgcaa taattgtcta gtaattgggg gaagaaataa gcttattcct tatctcattt 22740
ctttttttct ttttgagaca gagtctcact ctggtagccc aggctggagt gcagcgatgc 22800
gatctctgcc cactgcaacc ttgctctccc gggctcaggc gattctccca cctcagcctc 22860
ccgagcagct gaactacagg cgtgtgccac cactcccggc aatttttttt tccattttta 22920
gtagaaatgg ggtttcacca tgttgcctgg gctggtcttg aactcctggg ctcaggcaat 22980
ccacccgcct tggcctccca aagtgctagc attacaggca tgagccaccg cgcctggcag 23040
ctcatttttt agactaaata aattggagat ggctaaaaga tttttatgta ggccaactat 23100
gtttttaaaa agtttttttt tttaaggata tctgctggaa ccaatcatgc caccaaccaa 23160
agatgcaaga ctataaaaca tacccagttt ttcaaagcat ttaaaaatta ttctaaaaat 23220
attttttctc cagaaatttt gcattgattc cctgaagaag cattaatatg ggacctgact 23280
tataaaatga tgaactcaat ctccccactc aaggtaggag tctctcagat ttaaaaaata 23340
agcatcctag tcctcttgtc cctgtaaaag ttaaccctta cacctgaaac accaggagac 23400
tggcggttgt ttgcataggg gttacaatta aagttgagct acctctgaca tctattaaca 23460
ccaaaattag taaactatgc atgtatggag acttttatga ttgaacttgt ttattgagtc 23520
aagagatata gtttacaatg aaaatttggg gcatatcaaa atgaccttgg cttagcttag 23580
catttgctga tgttaactat tttcttcatt gggctgattt tagttgctta ggaaaaatac 23640
aaacacacac actttaaaat tatattaaaa tcccgtccta aacctcagag tccagaaccg 23700
catcctaaca ctggtcatgc ataatatgtt taaatttttg tgctttaaaa actacaaata 23760
aggaatgtat taatagttcc acaatcaatg gtcagttagc cgagggaaga ttagcatagt 23820
taaagactta aaatggctta acaacatata tcaaaaggac aaaataaggg gaacagagtc 23880
tagaaatgag gaaactggga cacaggcaaa aaaaaaaaat gagaactggg acatgaataa 23940
cgcaagggat aagactaata cacaaaacac cccaaataaa tagccagcat ttgctgagct 24000
cttactgtga gcctgttcta agcactttac atatattaac tcatttcatc ctcaaggaac 24060
catctgaggc aggcactgtt atcatctcca ttttacagat aaggaataga cccagagagg 24120
ctgagcaact gggcctattc cacagctact atggtggaga tgagatttaa atctaatcat 24180
tggctccaga gcccatgcac ccaatggctg cactaagtga atgcatgcgc tatcaacgtt 24240
gccaaaagtg ggccacagct cggatctgcg ttttccagta gccaaagcag agagtgtgat 24300
cagacctcac tttaataagc aagtctcaag ccagagagag gtggtatcag gcagcaaaca 24360
ggctgctagt cgaaatccca cttcttctct gagtggtcca tacagtttta ctctacttgc 24420
ttacagaatg aaaatagctg gagttcaggt gcgctttcaa tgccctgttg tcaggattgg 24480
gcttttcaag tttatttttt gttgttgttt ttaatagact gtacttttta gaaaattttt 24540
agatttacag aaagattgag aggatagtac agagagttcc cgtatacctc acacccagtt 24600
tctgcaatta ttaacctctt acattcatgc ggtacatttg ttacaattaa tgagccaggg 24660
ccggccgggc acagtggttc aggcccctaa tcccagcact ttgggaggca gaggcaagcg 24720
aatcacttga ggtcaggagt tcgagactag cctgaccaac atggtaaacc ctttctgtac 24780
taaaaataca aaaaattagc caggcatggt gctggttgcc tgtattccca gatactcagg 24840
aggctgaggc acaagaattg cttgaaccag ggaggcggag gttgcagtaa gccgagatcg 24900
tgccactgca ctccagcctg ggcaacagag cgagactcca tctcaaaaaa aaaaaaaaaa 24960
aaaagaagga aggaaggaag gaaaattaat gagccaatat tgagacatta ttattactaa 25020
agtccatgct ttatgcagat tttcttagtt tttacctgct gtcatttttc agttccagga 25080
atgcattcag gatgccatac cacatttagt tctcatatct gcttaggctc ctcttggcta 25140
gactgagttt taatctactt tctgcagagc ctgagaactt tagcataatt tccttgaaat 25200
tacagctcaa tattttcaag cacttataca aacagcctaa tgttacgttg gcccataaca 25260
gtgtttcaag gtaataaact tctttgtttt ctgtgccgat tgaaagaact gctgcttagc 25320
ctcctgccag atgatgaact gggtacacac gagcattttt ccaggtaaag catatttcgt 25380
gcgacttctt aagctgcagc cttatatgca ataattgtcc atttacaaga cttatgttcg 25440
aatttcaggc actctgtttt cactaaccat atcttcaact ttgataagta ctgctttaat 25500
cactcagaaa atttaacttg actaattttt tttcaccatc agtttttttt ctgttgactc 25560
tttctccttt ttctgtttgc ccagaaacat gctcaggatt ctctcaggct ttaaaaaatg 25620
aaaaaatgtt tcctgcaatc tagttactcc ttgattctct tgttctgttt atcgctggaa 25680
ttcttgaaag cttggtgtat tagtcttttt tcatgctgct gataaagata tacctgagac 25740
tggataattt ataaagaaaa agaggtttaa tggactcaca gttccacgtg gctgaggaag 25800
cctcacaatc atggtggaag gcaaaaggca tgtcttacat ggcagcagac aagagagaat 25860
gagaaccaag ggatttcccc ttataaaacc atcagatctt gtgagactta ttcactacca 25920
caagaacaat atggggtaaa ccgcccccat gattcaatta tctcccaccg gggccctccc 25980
acaacacgtg ggaattatgg gagctacaat tcaagatgac atttgggtgg ggacatggcc 26040
aaaccatatc acctggccta tagcattatt tccatttctt ccccatcctt ttattcctca 26100
aaccggtaca accagacctc tttttttttt tttctacctg aaactgctct tttgagggta 26160
gctgataagt ccaaaatact gtcacctttt ctcaattccg ttccttctta tgcctttgga 26220
gcaattgact gtgttggttg ccccctcctt taaagtgtct ctcacttggt ttttatgact 26280
aatgatcatg attttctttt tcctctctaa acattccgct atctttttag cttcccttcc 26340
ccctcccatc ccctaaatgt ccttgtttcc cagaatctgc ctcacctctt tgacttctct 26400
atgccctgtc attcactcat gggtctttat tacattattg catctgtgtc aataactctg 26460
gtctttctct taagttccag tctcccattt tcaaatgtcc ccagacattt ccaattgagt 26520
atctctccaa tgtatttaac ctgctaaata tctaacacat aatctttccc atcaaatcgt 26580
ttcctcttaa gcttttctta tttcctatta gtactcctgc acttctccca ggagcccaga 26640
cttaaaacct tgaatttctc accataacct ctcttttgtc tcccataatc aattagtagc 26700
aagtgttatc aatgattact tgacaatatc tttttctatt tccctccctg ctatgatcat 26760
tcatctagca agaagagttg gccctttgta tctgtggttt ctgcatccct ggattcaacc 26820
aactgtagat ggaaaatatt tgaagaaaaa agcgtctata ctgagtatga aaaaatttta 26880
tttcttgtca ttattcccta aacaatacag tataacaact acagcattta cactgtagcg 26940
tatagatctt ataatctaga aatgatttca agtacaccat tatatataag ggacttgagc 27000
atctgtgaag tttggtattt gtggggcata ctgggaccaa ttcccccatg gatacagagg 27060
gacaactata tttactcagt gcttactaaa taccagttgg ccaatgtgtt tttctttttc 27120
tgttttcctg tctttagttt gccccttgcc aattaattca atagtgctgc caatgccagg 27180
tgtaccttca gaatattcta ttctaatttt gtcatctcca agcttaaaaa tatttaatgg 27240
gccaggcgca gtggctcaca cttgtaatcc cagcattttg ggaggccaag ggggggtgta 27300
tcacttgagg tcaggagttc cagaccagcc tggccaacat ggcgaaaccc tgtctctaca 27360
aaaaagtata aaagttaacc aggtgctgga gcatttgcct gtggtcccag ctactcacga 27420
ggctgaggca agagaatcgc tttaatctgg gaggtggagt ttgcagtgag ccaagatctc 27480
tccactgcac tccagcctgg gtgacacagc aagactctat ctcaaaacaa caataacaac 27540
aacaacgaaa aacatttaat ggctgcacct tgcctgtgaa aaatgcattt cttggccaga 27600
tgtggtggct caaacctgta atcccaacac tttgggaagc taaggccagg agttcgagac 27660
gagctgggat atataggaag acacaatctc tacaaaaaaa aatccacaaa attagtcagg 27720
cttagtgttc atgcctgtag tcccaggtac tcaggaggct gaggcaggat tcctcaagcc 27780
caggagttca aggcttccgt gagctatgat ggcacaactg cactccatct tgggtgacag 27840
agcaaggtcc tatctctgga gaaaaaaaaa aaagaaggca tttcttagga gagttcttct 27900
ctgtagagtc ctaagggttc catggaactc cttaaaagca tcagagtatg tgagtgcaat 27960
gggaggaagc atttagccag agcagttgtg ctcccattgc atattaattt ttaaaaaaca 28020
aagctataaa aaaaagttga aaactactac gttagcatca gcctgacatt taatggcctc 28080
gtaaatcaaa ccttaattga ctttttagcc agttatgcta ctagccaact acagacaaca 28140
cactttttaa ccaaattaga ctaatagttg tcatcagtgg aaatcaagtt tgccattctt 28200
ccatgccttt gctcacacca ttaccttttc tggaatgtcc tgtactcatc ttcctgtgtt 28260
gaactctata cccaacttta aaaacctagc tcaaagttca acacttccat tccatttcaa 28320
aaagagcttt cctcttcctt aaagtttaag aactcatttt catgaatctt tttggcattt 28380
attgcacaca tgcttgcttt gtgttatttg tgttcatgcc tcatatgccc ccaaggtgtt 28440
ttagactcct taacggcaaa aatgatgctc taaacacctt tctatctttc atagtgtctt 28500
agtctgtttg tgttgctata aaggaatacc tgaggctggg gaatttattt aaaaaagagg 28560
tttatttggc tcacagttct gcagctatat aagaagcata gtgtcagcat ctgcttcagg 28620
tgagggcttc aggaagtttc cacccatggt agaaggcaaa ggggagcagg catcacatat 28680
caagagagga ggaaaaaaag gaaggaagaa aggagggtgc cattctcttt caacaatcag 28740
ttcttgtggg aactaatggg acaagaggct gggcacggtg gctcatgcct gtaatcccag 28800
ccctttggga gaccaaggtg ggtggatcac cagaagtcag aagcctgaga ccagcctggc 28860
caatgtggtg aaactccgtc tctactaaag atacataaat tagatctagc tgggcctggt 28920
ggcgtgtacc tgtagtccca gatactcagg aggctgaggt aggataatca cttgaacccg 28980
gaagacagag gttgcagtga gcttgtgcca ctgcactcca gccggggcaa cagagtgaga 29040
cggtctcaaa aaattttaaa aactttaaaa ataatagagc aagaaagcac caagttattc 29100
aggagggatc cacccccaat gactcaaata cctcccacca ggcctcactt ccaacactgg 29160
ggatcaattt ccgtatgaga tttggaggag acaaatatcc aaactatatc acatagtaat 29220
gaacatagta ccttatctat agaaagcaat ggctagacaa ctgttgaatg gctaaccaaa 29280
tctgctttcc tatggtctcg ctctagaggg ggtcagtatg agtttctgtc aaaaggagaa 29340
aaaaaaatgt atagtcagtt ttgtgtgtgt gtgtgttcat gtaaaagaga tcaagagaaa 29400
agaacaagag aaatcatgaa aaggaggggg aatataagaa taatacatag aaaaaagcaa 29460
attatcttgt ttatcagtaa tacccaaggg ggtagaaatg gtaagtaata atccttcttc 29520
actttgtctg tagttcactt ttttgcacct ttattttgat gaattcacat cgaagacatt 29580
aactcattaa ggcttccaat atttttggag ataagaaggg ctgctatgct ctttatagat 29640
ggaaaacttg ggtcattaat aactcaaaca aggacataac aaagaaatgg agcataaact 29700
gccaggtcct gactgtagat ttggattccc agttggtgtc ttgtcaccct ttgttactct 29760
tcctaaagtt atgatctttt cttgtgcata ggaaattcat agtgatttcc catcaccctt 29820
gggattatca tagctccttt aaggtcccct ctatgcactc aataacatca acagtaagtg 29880
ttcttcgagc acttactgag tgtatatcat tgtgttctca cgcagcaccc acagatctca 29940
ccaagaacct agctgaagcc tgtagaatga ataggtaagt actgccatgc caatctggag 30000
tactcaagcg atgcaaatga ttcctttaat tgtacttttg caggcttgtc agttttgctc 30060
atggagaagt ggctactgca tccatgttat atctatgtaa tgttggactg cgaagcatca 30120
cttgactttt tccaagcaga aattacagct gatgacaagc tgctgctgag aaaatggata 30180
tttttctgaa ttcagttcta cgtggaaaca gctgactagt ttccattgct gtaagatggc 30240
tcttttgctc ttggttgatt ttgagtaatg gctttacttc tgtagaaagg agatttcatt 30300
tgaagtccac tcagggattt ggttcaacaa actggagtac aggtttcaga aaatatctct 30360
ttaatcctcc aataataaat tttctcatct ataattcctg gaacacttca tcctttgcag 30420
ccgagcatat agatagattt gttgctcact gtgttctgat tgccactttg acctgctttt 30480
tcaacttagg ttacaaatag aacagaatct ctctgatttt tctcattaat tgtttgaatt 30540
cccacttttc ctcattagca agaagtccag tatcttcctg agaacttcct tttctcaatc 30600
taggaactta cttggtccat aaggtaacag tcttatttct gactatcaag gagagaaata 30660
acaggagcca ttatcatctt catggtgtca cttttgaaaa ctggtcctct gtagatcttc 30720
agattcttgc gttagtccat tcagctgcta taacaaaatt gcatagacag catggcttat 30780
aaataacaga aatgtatttc tgacagttct gaaggctaga aagtcaaaga ttaagacact 30840
ggctgatttg gtgtctggcg aaggcccatt tgctcataga tggacgatga cctttcactc 30900
tgtctgcaca tggcagaagg gcaagagagc tctctgggtc ttttttataa gggcactaat 30960
ctcatttttg aggaccctgc ccccatgact taatcacctc ccaaaggcac tgtctcccaa 31020
taccatcacc ttgagggtta ggatttcaac atatgatttt ggggggacag aaacacgcag 31080
tccatctcgc ttgtccactc catggtggta ttcttgctgg atcagtttcc tccttggggt 31140
gcatttgtgt tccatgtcta acttgcaagt tatagcaggc ccgatagcaa agtattccaa 31200
tgttggtatg cagaggcatt gaataatcag aatgaaccca cgccataaac aactggtaga 31260
gctgcagaga gtaccagctg attatgagcc ctgggtaaca gtggttttta gttcctatgt 31320
ccgtcagccc ttttctccca tagtagcccc actgtgttga agtggctgaa tcgacagaag 31380
cttccagctt gggccacatg ctcatggaac caattctcct tatgagccgt acaagagctg 31440
ggttgccatt ctggataccc tctttcttca agagatttta tttcaaggat attttttctt 31500
ttatcaacta cagggattat ttagaatctt agggcagtgg tgcccaacct ttttggcccc 31560
agggacaggt tttgtgggag acaatttttc catggaccag tgtcaggggg ctgggaggca 31620
tggttttggg atgagtcaag tacattacgt ttgttgtata ctttatttct attattatta 31680
tattgtaata tataatgaaa taattacaca actcaccata atgtaggaat cagtggggag 31740
ccctaagttt gttttcctgc aactagacag tcccatctgg gggcaatggg agatagtgac 31800
agatcatcaa gcattagatt ctcataagga gtgctcagcc tagatccccg gcatgtgcag 31860
ttcacaatag gatttgctca cctatgagaa tctaatgcca ctgctgatct gacaggaggt 31920
ggagctcggg cagtaatgcg agggttgggg agcagctgtc aatatagatg aagctttgct 31980
cgctcgcctg ccactcacct cctgctgtgt ggtccacttc ctaacaggtc acagactggt 32040
actggtccat ggccagggag ttgggaccct gtcttaggga gtaggggtgg agttcccttc 32100
acttctagaa ggccctggat tagtatccca gagctgtcat tacagagtat cacaaaccag 32160
gtggctaaaa acagacatga attctctctt atttttgatg gcttggaagt ccaaagtcaa 32220
ggtgctgcca gggccatgct ccctctgaaa tgtgtagggg agaatccttc cttcctcttt 32280
ctagcttctg gtggtttgct ggcaatcact ggcatcgctt ggcttgcagc acttcaacat 32340
ctgcctttac tgtctcatag tgttctcccc tcatgtctcc aggtctctct gtctctcttc 32400
tttgtataag gaaactagtc atattggatt aagggccaac cctactctag tatgacctca 32460
tcttaaggtc acatgcaatg actattccag ataaggtcac attctgaaga actgggagtt 32520
aggacttcat atcttttgaa ggaacacagt tcaaccaata acagcccctg tactgtttta 32580
caaataggta ttcctctcct tcccaaagtt cttcatagca gagacaactt gtaccaaaag 32640
gcaaaatacc ttattatgta accttaacct aggatcatag atccctactt gtctggtgct 32700
tttataagcc acagaaccac ccgggaaatc attattaaga caaggaaagg ccaagtgcag 32760
tggttcatgc ctgtaatccc agcactttgg gaaattgagg cgagtggatc acctgaagtc 32820
aagagtttga gaccaaactg accagcatga cagaacccca tctttactaa aaatacaaaa 32880
attagttggg catggtggca tgtgcctgta atcccagcta ctcaaaagac tgaggcagga 32940
aaatcacttg aaccgaggat gccaagatag cagtgagcca atatcgtgcc actgcactcc 33000
agtctggatg atagagcaag atcctgtctc aaaaaattaa taaataaata aaaagacaag 33060
gaaagccttt tccaaggaga cccttctgct ttgctagttc agagaacttc tctttggaga 33120
aaacaaacac ccagtccatt agcagcaacg tcagggattg aattcttagg gcagcaggct 33180
gggcacagtg gctcatgcct gtaatcccag tactttggga ggctgagatg ggtggatcac 33240
ttgacatcag gtgttcgaga ccagcctggc caacatggtg aaaactcatc tctacaaaaa 33300
atatgaaaaa aaaaaaaaag ctgggtgtgt tggcttatgc ctgtagtctc agctacctgg 33360
gaggctgaag caggagaatc acttgaaccc gggagttgga ggttgcagtg agctgagatt 33420
gccctactgt actccaacct gggtgacaga gagagactcc atctcaaaaa aataaagaat 33480
tcttcgggca gcagtctttc ctccacctca tagaccatgg aggtgagcca gctctgacaa 33540
accatgagaa caatggcaga gacatacctg taacgtaact gactggggca aagacaaagg 33600
tgaggaaaat gacaagtttg aggaactatg agaccaggca gtggggaaca ccactagcag 33660
aaatgatgga agttctcaag aataacaaca gagaaataga ccatggccag agtctagaac 33720
cctccaggga aaggagatgg gctccagagg cagaagagga cgttgaaggg aatggggagt 33780
gggtgaaata tatagacgat ggggaccacc caagagcagt cgctattgca aaactgagga 33840
gaaggagagt ctggaggggg tggtgggaag ctgggtctcc taaggaggtt ttgacaaaag 33900
cagtcatgga gcgggcttag aaatcacagt tggggacagg gtaaagttcc tcgggatata 33960
gaggatgaga ttagaagagg ttccaactag ggtagtgtgg agaaaagcac tattgaccca 34020
aaaaggaagg agaatgtggg tggaagtggc agagaaagag gggtttgagc agagagtggt 34080
gatttttcta atgcagagtt gtgggaggtg gagtgcaggg agccaggctg ggtggctgtg 34140
ctgatgtgat taagcactta ctgactgcca ggcaatgggc taagtacctg agatgctttg 34200
tctgttatcc ctcccgaaac ccctctgagc aggtgcagtt attattctca cttcacagat 34260
aaggaaattg aggcacagag aattgagtaa cttacccaag gtgacatagc tcatatatgg 34320
taaagcaggc tttgaactca gtctagctcc cgaacctaag cttgtaacta ctatgctttt 34380
cccaaaaaaa gggggctggc acaaaaagag ctgagggggg ctgggcatgg tggctcatgc 34440
ctgtaatccc agcacttcgg gagactgagg caggtggttc accagaggtc aggagttcga 34500
gaccagcctg gtcaacatgg tgaagccctg tctctactaa aaatacaaaa attagctggg 34560
tgtggtggtg tgcacctgta gtcccagcta ctttgggagg ctgaggcagg agaatcgctt 34620
gaaccccaga ggcggatgtt gtagtgagcc aagatcatgc cactggactc cagcctgggt 34680
gacagagtga gactccatcc aaaaaaaaga agagctgagg tgatggccac catcagcatc 34740
agcctggaag ttatagcagg atgctaagtt tctctaaagc tgtctttctt aggacttgaa 34800
aaagataact tgggtttgta tcccatctct gccattagta gtttactggc tttggataaa 34860
ttacttagcc ttactgaacc aactttggat ttttatagag atactgtaat gaaaggaata 34920
aggtatcagt cttagcagag catccagagt gttcctatta aaacctaaat catatcctgt 34980
cattgctctg ccccaaacca ttcaatggct tcccaactca aagttaaaaa ctcatctttc 35040
cagtggcctg caagagccta tgctatccgg tgtctgacct catctgttgt tcctttctcc 35100
ctccctttct tggctccaga cgcactctgg tctccttgct gttccttgaa tacaccaggc 35160
acactctttt cacctgaaac actttacccc agatatctta gcttactctc tgcctccctc 35220
aattcattga tgaaatgtct cagtgaagtc ttctctctct cctctgtaaa agtatactct 35280
ctgttcccct tctttactgt tctagctact attgctgtgt aacaaatcac tccccaaatt 35340
taatgagtga aaacatcagc catcatctta tttctcacgg tttctgaggg tcaggaattc 35400
tggaagggct cagctgggag gttctggctc tataatctct tatgcagtga gagtcagatg 35460
ctggctaaaa ctgaaacaaa gcagggttct agtagctgag ggctggctgg gtctctcaga 35520
tatagttcag atctcctcca gggggtctct ccacgtgggc tagtctgaac ttcctcacag 35580
catggtggcc tcagggcagt ggactctgca tagtggctga aggcttcgca gctgagtatt 35640
ccagcaagca aagtgggagc tgtattgcct catatgaccc aaccttggaa tccacacagc 35700
atcacttccg tgtattctac gggttgaaaa gtcacaaaaa ccaaccagtt tcaaggagaa 35760
ggaacagaga tcacatttct caattggaga agggtcaaag tcacattgta atcagagcct 35820
atgggatacg aagtattgcg gtcaggtatg aaaaatttga tttgctgcat ctgctttact 35880
ttctccacag cgttcatgat ctgcttctca catgatattg acttacgtca tttctgcatt 35940
tcctgtcttc cacactaaaa tgtcagcctg ttttgttcac tgctgtatcc ccagagccta 36000
gcacggagcc cagcatgtag tggtatccaa taaatacttg ttgcatgaat gaattctgtc 36060
ttttaatcct agctataggt ttctaagtta aatattacta taatcatctt acagacgagg 36120
gaaatgaggc tcaagaagat ttggtaactt atgcgggatc actcagccac ataatggaag 36180
agacagcatt gaagtacaca tgcttgctct gtctgctctt ccaagctgct catcacacag 36240
ctgcacctct gaggacttcc ctccccagtc cacctccacc cttacccaga gacacacatg 36300
gccacaatcc actagcagac caaaattcaa tttttcccca gttggttgca ctcaagctga 36360
gagcaaagca attgcacttt aaatcccctt acagcagata tttcagagca tgttcggaag 36420
aacccatcac acttggcttt tagatcttat ttctggtttg ttacaaaaac acaattaaat 36480
gaaaggttag gtagcttttg aatggccagc tcaaagtttt ggcttatttt tgccttgctg 36540
tctttatagg cattttacca atatttatca ctatttccct tagggaaccc ttagatctgt 36600
gatatttgaa ataataaagc ctctccattg gccctttaaa aggtttgtgg taaaaccaca 36660
ccattaacat tcacagttcc ttatttatga ggcctgattg cacttatttc catatttctc 36720
actgtttctc cgatgaggat ttcacataat agtgtttgaa ggctaaagac ttcaaagcag 36780
attctttact atttttatct tgaaaaatat tcaatatttg tgtaattaaa gtgaagtctt 36840
cctagagaaa atgacaactc aaataatctt aaatgtacct ccaagaaaaa agctgtcaaa 36900
gtgacattta gtagtagagt cacattctct aaggcctttg cttctccttc tgagttctta 36960
tcatctttga aggttatgtc atggctgact tcaaatcact tttaaaatta ttatggcctt 37020
ctttaaatgt gagttctgaa ggtgaggggc tttatctttc ttttgctcca gattttttct 37080
accgcgtcat taccaagcat cttaaaacaa aacctaaaaa caaaaatctt ccttgacctg 37140
gtttttccca ctagctaaca tcctattttt atctttccct ttgcactaaa ggtttttaaa 37200
cggatcttta taccctctgt ctccattttc tcatctgcta acttatatgg caaagattac 37260
cactgccttt caacataatt ggccaatcta cagaaagttt tcaagttctc tttttaattg 37320
accacctcct gcctacctcc ccacctttga catcttgctt ctcacttggc accttaccca 37380
gtgttcaaga ttccctcctt taggatgtct tcagagcagc tacacagttg gtactataat 37440
ttatacatcc ttgtacacag ggcttgctgg gatattgatg gagagaagga ggaaactgga 37500
agtagttcag gccagagcta gggaaattga cccatctcca ggtctcaggt ctgcaagggg 37560
agctcacagc ttaacacatg gagtctagaa acttgtgctg gaccttgacc aacaccagcc 37620
catggagtcc aatacagtgc tcaataggga tttccaggaa attgctatat ttattcaaag 37680
agaacttacc aagtgtcagc tacgtgttgg gcattgtgtt aggcacaggg accacaaaga 37740
taagacattg tagctttcct taagttgctc actgagtaaa tagagagaca gaaaggtaaa 37800
caggtaagtg caaaaataca tacaattctg caatagtgtt catagtggct atggagagaa 37860
cgctcactaa ctttgtttaa acagttgttc tttcaaggat ttgacatgga tttgattgga 37920
aaagcatgat accatttttt gcaattaaac acaggaatac ataaataaaa tgcatcagta 37980
ttttttacaa atagctacta agagctacta gaaaacctgg gaattcttaa aaccttacca 38040
tgctacttgc tctaaaatat tttattttat gttattttgt acatttcttt acctacacaa 38100
acaccactgt tttcttcatt tcttagtcta tttaaacctc acaccctttc agcatctctt 38160
aattatttac taccatctgt tagttctcct gtcctgaatg aaacaaaaat ggcagaatgt 38220
aaaacgaggg cgaacagatt tttgacagga agtattcaga ggtagaagga aatagtcaag 38280
acacatatga taaacgaaaa caataataac tttatacata acaacttata gacacattta 38340
aaaagtttaa gatctcaaga gctatgtctg aatagataga agtaaaaact ctattaagta 38400
attaggaaaa taacaagaac agtgaatttc ttaatgaatg gcatgtaatc aaaactgtac 38460
ttatcgtcta attcataatc ttgaatgttt ttattttatt tatttatttt tttatttttt 38520
gagacagagt cttgctctgt cacccaggct agagtacagt ggcgtgatct cagctcactg 38580
caacctccac ctcccaggtt caagcgattc tgctgcctca gcctcctgag tagctgggat 38640
tacagaggcc tgccactgca cccggctaat ttctgtattt ttagtagaga tggggtttca 38700
ccatcttggc caggctggtc ttgaactcct gacctcatga tccaccagcc ttggcctccc 38760
aaagtgctgg gattacaggc gtgagccacc acgcctggtc gaatgttttt attatttgaa 38820
gagacaacat gggccttaaa tctgtcttct atttgacaga ctttgatgga gtcaaatccc 38880
aatgctgcca cttactgaac ggccttaaat gacttagtct ctctcagctg tctttctgca 38940
tatgtaaggt ggaataatga tggcttcaag gaggaataaa cctatgaaaa gtgttgagga 39000
tagtgtctga tatgaaataa ggattcaaca agtagtagct gctattgaag atttaagagt 39060
tatttattac aactatttaa taaaatttta aaaactaata cacttaaatt attaaagagc 39120
tttgaaatgg gccaggcgca gtagctcctg cctgtaatcc caacactttg ggaggccaag 39180
gtgggcggat cacctgaggt caggagttta agaccagcct ggccaacatg gtgaaaccct 39240
gtctctacta aaaacgcaaa aattagccag gtgtggtggc atgcacctgt agtcccaact 39300
actcaggagg ttgagggagg agaattgctt gaacctagga gctggaggtt gcagtgaccc 39360
gagatgtcac tgcactccag cctggcaaca gagcaagact ccataaagac aacaaaagct 39420
ttgaaattgt gtaaatgagt tgtacctatc ttcatttaag aaattcatct ttgttcatct 39480
atttttactt gacatgagag cttccagcaa tttttaatta agccctcaca gattttatgt 39540
cactggctat gtgataaaca aattatttgc taaaataata ttcttgcttc ttttttaagg 39600
aattgtctcc ctagaaacgg tttgtaccaa acaatacact gactttacac aaaatcagat 39660
ctgattggca acagttgcag atgttttcaa aggattttca tttgagaagg ggcccatttg 39720
ggttatttag attctaagaa ctgaaactgc tttgttctgt ttttctggct tctgggagag 39780
gaggagacat gaattcagtt agcaccttgg tattttcttt atccttcatt tcaatacaga 39840
agatgcttca tatgcacagt ggtgtcaggt cacatcaaaa gaaagagaaa cagtttcttg 39900
gtttttaatt ttcaaccgga aaggaaaggc acccattttg ttccgctcta attagccagt 39960
gcatgactta gagagcaggc agatgctttg aaggcgtggt aacacaggtc ttcattaatc 40020
tccacgcagg acttgcactt ctactatgcc taggctgaag aaaatggctc aggaagatga 40080
acaatctcac agagccctaa ctaactgaag ccaggtgtta taaagcacaa gtcaagaggg 40140
tgagaaacta acgttcttga aatctcccac ttctttctac gtcagaagag ccaagctgat 40200
tattttagtt ggaatttaga aatttttaaa aattattcta aagtcatgaa caagcctaat 40260
tataaagata gttgctgtga aggtgctgaa ataactcgat tttaccaacc ccctcttctg 40320
gaggaagcca gaatggaatc ctgtagaatg ttcactctac caacgaactc ttgtttttct 40380
aatgaggaaa cagaggccca cagtagtaaa ctatcttaac caagacaaaa tgactagtgc 40440
tctggtcctt ttattaagca ctaaaatttt gatccaataa taaatctgtc cagtagaagg 40500
agtttcccta atgtactggt tctaacttgt tcccttcaag gggccagtgt cccgtacaca 40560
tagctaaatg ggacttctct tcaactacca ttacccagag ggcagaacct aaaatgctgt 40620
gaatgacatt ctgctgttca catctcagca gcagtgttgc atttgagctt ctgcagggcc 40680
acccaggacc tatatctgct cagatgttta actcatctaa ttcagtgaac acttcattct 40740
agttaactga acatctactt tgtacaaggc actacagcgg ttcagagatg aataaaatca 40800
tgagattcca ctgtctccta taaaccatca ctttgggaaa ttttagaaat gtgggtaagc 40860
tccagggctt cctgcagcgt agaagtcaca aactcaaatg cctgcagagg cccagctgac 40920
aacataagta aatgattctg gctgggcgga aaacaattac gggtgggtgg gtttccagct 40980
ggggagtgca cgcctgtgtt aaaggacagc tgctactcat ttccagccaa ctgtgttccc 41040
atgtagaact gcggcccagt gtagccagta ccgaagattt ctcagaaaaa gccggagatc 41100
tcaatgttag tgtaaaatct ctcaaatttc caagaggatt atatggggca aaggttctca 41160
gatcagtttg cagtctctta cttagcccat gtgcagagca gtcgtagagg gtagcatgca 41220
gtgtcctaca taataattct tttttatttt attttatgcc ttcctccttc ctgtctctct 41280
ttaacctttc ttcttccctc aggctggctt cttccctcag cctcgtccga ccccagcctg 41340
ggttcaatga acattcggta aaggaacacg gaatgtcaag cgcattagag acaaccttga 41400
gacacattcc tcttgcggta agcacttcac tgtagatttt taattttaaa caagacaatg 41460
tttacgactt gcttctttca gggaagagcg atatcaattt tagtgaacac ttcaaggctg 41520
agatacgcta ggagagtcgt gtggtgttgc acagcaaaga attccacttt gaagcgagtg 41580
ggaaaaaaag catcaaatgc cacatgtaac tcaccgcctg aagggttaca ttggtatgaa 41640
acctgggttt aaaaagggac cgaatagact agccattaaa agacctgcgt acaacctctc 41700
tctctctctt tgagagataa tgtatctgga caataaacat gaacagagtg gagtctatcc 41760
tgtttaaaac attgcctact gtacaggcac caggagctga agggtcagaa tattagcagt 41820
gggagcttga ttagaagttg atgagagatg ggtagtagga ggaaagagtg agatagagga 41880
agaggacatg ggggttaccc gtaagtggag agtagaaaag tagaatcagc tggccatcaa 41940
agggcgtggg actgaggaac agtatggcat gtattaaata tactaagcgc tgacattgga 42000
ggagaactag gaaggtaaat gaaatcaata ggggatgatg gagaatagtt aggtgtgcag 42060
ggattagggt tatgatagaa atacatgtga atacatgcag tattgtcctg gaaaatggtt 42120
aacagttggt tctcctgggg ggtgagggga agccctgatt tgtaatattt gcctatttct 42180
gtggtgcaaa tactcccacc atgaccagtt tcaagctatg aatgtgaatc acaaaagcag 42240
gttgggagga gatgcgcaca tttgttcccc ggcaaggtgg aaggtaagga aggtgaaatc 42300
aacaaggtca aagaaaactc aagatttcga ggtgcctcag gtctgagggg caatgaagtc 42360
taggaatggc tgtgctgagg tagctgaaat agaagtgact gcagaggtca tgaagctgaa 42420
gaggtgaaaa cagaaattag aaaggcaaac ccccaccgcc caacccccac ccctgcagcc 42480
agtttctgag ggtgacaata gaggaaaggg tggagatgga gttcaggtcc agaagccata 42540
gaagcgagtg tgacattgtg ctcaaggtca gcacatgtca gtgtggggtg tcacatgctg 42600
ttgtgaacca tcatttatca ccaattatgg aagacctcct atgggcatct tgccatatgc 42660
attataaaga tgtgtaagaa gacatttccc tccacttggt gaggagaatt agggctgtac 42720
acagatactg tagagtgcca tgtgcctggt acagataagg tgtgttagag gttaaaagat 42780
gaggctctta atattaatga tagatcccac ttacctgagt ctgacttaca atgtgcctag 42840
cattaagtgt tttacctgca ttccctttga ccttcagaac aacccatttt acagataggg 42900
aaattgggtc agaaagtttc agtaacttat ccaaggtcac acaattggca agtgccagag 42960
ctgagccagg aactgaggtc cttctaacac caaacagctt gtctccccaa tcactgtgct 43020
attttccctc ccccagaaga taatactctg atggaaatga aggatagtgt aataggagat 43080
tcggtgttcc tttttttaaa aaaaattcag cttgcatatt cctaaagagt caattcatgt 43140
ttaaaaaaaa tttcccttgt gcttgcatgt gacatgtatt tttaggatct gctgttagca 43200
agtgtatttt tgtgtgattg agtgggagag tgggaaaagt tttgcagagc tgttgaagcc 43260
agaatgcagg ggggctgcgc agcagagact gtaaaatctc tgccatctca ggtcttggaa 43320
caagcacaaa gagatgtgtt ctcgatttat tattctatgt acatccccag atgaatgact 43380
agttaaaggt attgttaaag cattttaaat gacccacttc cagcagcgaa caaaatcact 43440
tgctgtgcca agccaactgg catttctgag atgataaaac cacaaagtga ggaaaacgtt 43500
aaaactgcta aagcaaaaat gatacacaat aatggagaag gagaaaaatt gagctttatt 43560
gtctgcctag gcagatggct gaccactagg tgggctcggc gtcacgtcca gggtaattgg 43620
ttgctggggt gtttctggcg aggaagattc acgcttcagc tcggtccaca agatcctggc 43680
tcattctttc ctagattcca ttttctgcct cctctccatg actgggtctg atggttgatc 43740
caaacgggca attgaaatca gaaggttacc tttaccttaa aatgcttttc tggaaataaa 43800
aggacatgaa aagtaactaa ggaccggatt tcctagccgt ctttctctcc tgcatgcgca 43860
atttatcccc agatataaaa ttgcctgctt tgataattat accctctaaa tgaggggcaa 43920
gtggctaatt atgcccacat gtggccgatt gcactcccca ttagccaatt atgtgctcaa 43980
ttatttgtgc acatgaataa ttgcactcat ggaaaatagc ggccctcctt tcaaatcctc 44040
gtgcttggag tggctgatgg agtaattgtc acactggaaa tgcacttggt ggggagggaa 44100
agagtatcag ataccaggaa acgcataagt gaccagagct cgcagatgtt cactgccaca 44160
aatggcctta ggagccagag agagcgggaa ggaccacagg atggaacggg ccagcctgtg 44220
agttaggaag cctgcttctg aagttgcctg ggcagctcat gtgcggtgac cttgggcaag 44280
tcattaactt tccttcaggt ctaactggtt ctgcatacac aatgaggatg gtaataacgc 44340
ccaattccca tcactatcgt gggatggatc agactattta aaaggattta caatctgctt 44400
gggtaaaagc tttacataaa tatgaggcat tatcatgtcg cttggtacat ctccaattat 44460
gaaggaaggg taatgaccct ccacagcaat gcaggactcc tggtttggag ggagggaaag 44520
tttgagaagg acaggaagct tgttgcccca gcactgatgt ttctactgag gtaccagaaa 44580
atgtcatgtg gtcatacaga attcatttat tcattcaaca aacatctgtc aattgttaca 44640
ctgtcctgag aatttggaaa aatgatgaaa gactcagtcc tgccttagga ggtcactggc 44700
acattggccc gggcccctgt tttgggcctt ttactctgac ctgtgctgat ttgcaaatag 44760
tgggaaattt tatctcaagt ctaggaaatc tggcatgcat tttcacggtt tgattgccag 44820
gtacattcga tggcaatgag tcttataatg tttggttacc ttcatttacc taaaaactgt 44880
ggttgttgct gtggttgttg tttttgttgt ttttgagacg gagtcttgct ctgtcatcca 44940
ggctggagtg cagtggcatg atctccggtc actgcaaact ccacctccca ggttcaagcg 45000
attctcatgc ctcagccccc tcagtagctg gattacaggc gcgcaccacc atgcccggct 45060
aatttttgta tttttagtgg agacagagtt tcaccatgtt tggccaggct ggtctcgaac 45120
tcctgatctc tggtgatccg cctgcctcgg cctcccaaag tgctgtgatt acaggcgtga 45180
gccactgtgc ccagccagaa ctgtggtttt aatgacaatg ctaaaaagtg gtatatgtca 45240
cagtgtcggg tggggctaag aggcacattg ctgcagtgat ccatcattca tttcccacca 45300
ttctcgcctg gattagcgca gcagctccca gagaggcacc tcactttgac cttcttcctc 45360
aaagacattc tctgtgacct gcctggccct tattacctct ctagctttgc cacttcccta 45420
tgtctccatc tcccctctca cacgtagtaa gaaagagact ctacctccat ggaagttaag 45480
gagaggtttc acagaggcag gattgcttat tagtcttcaa agatgaggta tttgctaaat 45540
gaatgagaca aagggattgg ggccacatta caggaaattg aggtatgtaa tagcctggtg 45600
caggttaaga gtgtggactc tgaaaccaga ctcagcctgg aattgaatcc tggctgtgtg 45660
atgttgggcc agtgacttaa cctctctgtg cttttattca ctcttctata aaatggggat 45720
tataataaac ctaccttata aggttattat aagagtcagt aaatataaaa atagaagttt 45780
ttggatgatg actagcacag agtaaacact tgtttgccat tatttttatt acttgactaa 45840
aaatatacca aaaagaccat ccaagaaaag cctttaagct gctagtgcag aaagattccc 45900
cttgtgtttg tgtgctgggg ggtcagtggt gcctgtggcc cactggagag gagacagcta 45960
tggctggagt gattctcaaa cttcagaatg tctaaaatca tcacatggac aacttattaa 46020
ggaaagcaaa tgcctgggct ccatcctcag agagtctcat tcactgggtc aggatagagc 46080
ccaggaatct ttaccttaaa gaaccatccc acctcccacc tcatatgatc cttatgcagg 46140
tgatctgggg gcccacactt tgagaaatag actcaggtca aagtgggctc taactgcatc 46200
tcatttctta cctggcatat ctaatagtag agaagaagac aatgctaaga tttttgttgg 46260
agatcttttg ctgggattgc tgcttcattc attcactcat ttatttattt atttatttat 46320
tttgaaacag agtctcactt tgtcacccag gctggagggc agtggcacaa tctgagctca 46380
ctgcagcctc aggctcctgg gttcaatcga ttctcttgcc tcagcctccc gagtagctgg 46440
gattacagtc atgcaccacc acgcccaact aattcttgta tttttagtag tgacagcgtt 46500
tcaccatgtt agctagactg gtctcgaact cctgacatca ggtaatctgc ctgcctcggc 46560
ctctcaaaat tagtagctgc aattacacgt gtgagctgcc gtgcctggcc tgctgtttct 46620
tttagttggg cctcttctgt aatagagtgt gagaattctg acttgctgca acagtctgct 46680
ttgaagcagg gctgtgttta cactggtcag atgtggaatt gtggggcaca cttagcagct 46740
tccttctcta atttttctgt attttcagga gaacaatttt aaaaaattta ataaaaatgc 46800
cttaaaaatt aacattatta taagatgaat cccatttttc taatcttgta aattaaaaac 46860
aatcataagc atatgagcac ctgcacttag ggaatcaagg tggcaaagct aaacacttcc 46920
agctctaggt gattcgcggc aatacaaatg gagctggact ttggccacag tgcaaaaata 46980
ttgatctgtt gttagatgct ctgaagtttc cacaaagaat tggttctgcc tgctgtgctt 47040
cagtgcttaa gggaagtggt tcctcaaaat gttagttttt aagcccagct ttcttaaata 47100
ggaagattct aatagtagca aaaatataaa ctgcttctag gtttaaaaag gaccagcaca 47160
caatggttat cacacacctt tctcctcagg tgatgagtgg atgagtggcc tggtgtattt 47220
cataacatct cccagggtcc aaatgctaaa gcaattgctg aaaagatacc atgtgtaccg 47280
gaaccttgca gaggtatttt gttggcataa aaagaaatat tgatcatcta tagtaaaaat 47340
ggttctactt taatactact gagaaaagat tttcttttcc cagatctaca tcctgaatct 47400
tcatgaagac aagatcccct aaacttccac taacaccata atgtgtgctg tcctttgtaa 47460
tgtagtccac agatctcata aactgtcaga aatagcagag attgtaaggt catccacttc 47520
ccctgtaagg cctgcgtccc tcacttacat ccctaataac gtcctctaac ctctgctgga 47580
gggcagattt agctgccagc tgggaagagc tctgccctag tcaacatttt tatctgtggc 47640
tttcagatga gaacactgga tgcttatctg aaaaaagctc ctcaggctgg agggagggat 47700
tggctctaac aagatgcaat gtgataagaa taaaagcgaa gccaaactct aggcccaaag 47760
gctctagcaa cacacttttg agaaccttgg agacgagttt tggctgatgc gagcttctcc 47820
gcctgctaaa gtagcccatt ccatttggac ggctctagag gctggcatgt tcttctccac 47880
gttgtgttaa tgtactccag tttcttcctg ccatgaactg gcatgccctg gctcctccta 47940
ccttccccac tttaagtctt ccctccctcc ttctgacctt cccattccag ccacactggc 48000
cttttgtctg gtcctaacaa accatgcctt tcctgcctcc aagccctaca cctgctatcc 48060
atccctctgt ctgagagaca ctcccacccc ttcacaaagc ctgtttctca tccttccagt 48120
tcagatgtct tctcagcttg cctcaactga cctctttcag ctattctcac tctttgtact 48180
ctgttcattt ccttcctggc agtcaccata atttatcttt atttgaatca atttcttagt 48240
tgtattattt agttatttgc acactctgtc tctctgtgcc tttcttattc actgcaggct 48300
ttcttatgta agtaatttat ttacttaaat ttttaaaaat aatttcaact tttggccggg 48360
cacagtggct cacgcctgta atcccagcac tttgggaggc cgaggtgggt agatcagctg 48420
aggtcaggag ttcgagacca gcctggccaa catggtgaaa tcccatctct atttaaaata 48480
caaaaactag ccgggcgtgg tggtatgcac ctgtaatccc agctactcgg gaggttgagg 48540
gaggagaatc acttgaaccg gggaggtgga ggttgcagtg agctgagatc acgccattgc 48600
actccagcct ggggcacgag agtgagactt catctcaaaa aaacaaaaaa caaaaaaccc 48660
ctgcttttca gaggggctga actaatttac attctcacca atagtgtata agcattcccc 48720
tttctctaca gcctcactag catttacttt tttaaaaaac tttttaataa tagccattct 48780
gactggtatg agatggtatc tccttgtggt tttcacttgc aattctctga tgattagtga 48840
tattgagcat tgttttatgt ttgttggctg ttcgtatgtc ttcttttgag aagtgtcttt 48900
tcatatattc tgcccatttt ttgaatggag ttgttttgtg cttgttgaat taagttcctt 48960
atagattcta gatattagac ttttgttgga tgcatagttt gtgaatattt tctcccatcc 49020
tatagttctg tttactctgt tgatagttcc tgttttgtta tgttttgttt ttttgctgta 49080
cagaagctgt ttaatctaat tggtcccact tgtcaatttt tgtttttgtt gcaatggctt 49140
ttgaatttta ataataaatt ctttcctaag gctgatgccc agaacagcat tttctaggtt 49200
ttcttctagg attcttatag ttcaaagtct tatatttaag cttttaatcc acctcaagtt 49260
aatttttata tatagtgaaa tgcaggggtc ctgtttcatt cttttgcatg tggccagcca 49320
gcaatcccag aaccatttat tgaataagga atcttttcct cattgcttat tttgtcaact 49380
ttgtcaaaga tcggatgact gtaggagtgt ggctttttct gggttatcta ctctgttaca 49440
ttggtctatg tgtctgtttt tgtatcagta tcatgctgtt tttgttacta tggtctcata 49500
acatagttta aagttggata atgttatgcc tctgctttgc tgtttttgct taagattgct 49560
ttggctattg aggctctttt ttcacttcat atgaatttta gaatagtttt ttctaattct 49620
ttgaaaaatg accttggcag tttgatagga atagcattga atctatagat tgctttgggc 49680
agtatgctat tttaatgata ttgattcttc ctatccatga gcatggaata tttttccatt 49740
tgtttgtgtc atctactatt tcctttagca atgtttttta gttttccttg tagagatcct 49800
cctaggtatt tcatttttta tgtgactatt ttaaatggga ttgcattctt catgtggctc 49860
tcagcttgaa tgttattggt gtatagaaat gctacagagt tttgtacact gattctgtat 49920
cctgaaacct tactgaagtc atttatcagt tctaggagcc tttggcaaag tctgtagtgt 49980
tttctaggta tagaatcata tcattagcaa agaaagatag tttgacttct tcttttccta 50040
tttgaatgcc ttttatttct ttcccttgtc tgattgctct tccagtacta cgttgaatag 50100
gagtgctgag agtgagcatc cttgtcttgt tccacctctc aagggaaatg gttccagctt 50160
ttgcccattc aatatgatgt tggccatggg tttgtcacag atggctctta ttattttgag 50220
gtgtattcct ttgatgccta gtttgtcaaa ggcctttatc atgaagggat gttggatttt 50280
attgaaagct ttttctgggt cttatttggt gaattgcatt tattgaattg tgcatgttga 50340
gccaaacttc catcccaggg attaaaccta cttaatcatg gtgttaactt tttgatgtgc 50400
tgctggattt ggtttgctaa tttttttttt ttttttaaaa tggattctcc ctctgtcccc 50460
caggctggat tgcagtggtg tgatcttggc tcactgcaag ctccacctcc cgatttcatg 50520
ccattctcct gcctcagcct cccgattagc tgggactaca ggcacccgct accataccca 50580
gctaattttt gtatttttta gtaaaaacag gatttcacca tgttagccag gatggtcttg 50640
atctcctgac ctcgtgatct gcctgcctca gcctcccaaa gtggctagta tttttttaat 50700
tactattttt tctcaccctt gctgccatct tatgattttc tagtattttg ttgaagattt 50760
ttgcatctat tttcatcagg gatattggcc tgtaattttc ttttttcatt tcatctttac 50820
cacatttttg tatcaggttc atactggctt catagaatga gttcaggaat ggtccctcct 50880
cctcgaattt tctctgtaga attagtacca gctctttgtg tgtctgggag aagttgtatg 50940
ccaataattt aaatgcagtt aatatttact ggacaatttc ctccagataa ttgtatatga 51000
tttttggtcc accctgagtt gatacatgta ttttaattgt atcatggtat gaaaagagca 51060
agagtatttg gtcacctagt cttgcctata gatgtgccta atgattcaaa gtagatattt 51120
tgggagccta acaggtgccg tgactaggca gttttgtttt tttttttttt tgagacagag 51180
tctcgttatg ctgcccaggc tggagtgcag tggcatgatc tcggctcact gcaacatccg 51240
cctcctgggt tcaagcaatt atactgcctc agcctcccca gtagctggga ctacaggctc 51300
acgccaccac gcctggctaa tttttgtatt tttagtagag atggggtttc accatattgg 51360
ccaggctggt gttgaactcc tggcctcatg atccacccgc ctcggcctcc caatgtgctg 51420
ggcttacagg cgtgagccac cgcacccgga gattaggcaa ttttatattc ccaaatatcc 51480
aactcttctg acccgctttc tcagcctggg tgtatcaggc acaaggcctg ttcagattat 51540
gtggtctctg aagatatggc tctccagggt tgacaatgtg gataaggatt cacctggttt 51600
aggatttaca cattcgcctt gaatgtctgt tgcatcaagt agacagtcca tcccaacttg 51660
gccatttggt cagagctgta aggagacaag gaggtgggca gccgctgctg tgaactgctt 51720
ggacaaagac tgccaaatag ctatcagaca gtgttaacaa cagctgattt aggtttgaag 51780
ggggcagtct cttgggccac ttactatgct gcatcatcct ctttggaaaa tgctcttcag 51840
gtaactgcct aacagactga gaaaataaaa tgctcacaga gaaaaaagac ccggaaagtc 51900
tgacttctca gagctcagtg tttaggtgca gaactggatt gtgaaaggat ttttaaattt 51960
tttatattca ttgcagggaa cattcattta ttccatcctt ctccactccc acctgtctgt 52020
cgttgtcttt gtctctgtct ccccacctct ctctctagac acacacacac acacacacac 52080
acacacacac acacacacac acacacacac acacacacac acacacacac acacacccct 52140
attcattgcc aacagtaata gagttgcttc tttacttctt ggagagaaaa gcctcaatct 52200
gaggaagctg tgctgactag ccttgctctt aatcatggag acaatgcttt atgcctttat 52260
ctttgcacag ctgaaagcca tggcagaagc agtcctctaa acgaaataaa atagaaaggt 52320
tcctgctaag ccctggcaaa tgcagccttc tatccctccc ccaacactca cagcttctga 52380
gcaagatgta gctgccttcc aggaggctgg gtgatgggca ataatgagca gagccacgtg 52440
aaggaaagat gggtgaagaa atgtgtgtgg aggtcatgct ggctgcactg accatgaaac 52500
aaaggatcta cccctctagt aactgcccta ctcctttggt aactgttctg aaattataac 52560
ttgccagaag ttcagaagga cctagtgcag gtattagagg aaattcgtaa gattgagcca 52620
tttattcctg cacagataca taataatgga cacgggccat ggtggccagc attcttgctc 52680
ttgacaatgg tgaagggaag ggttgtaggt catggctatg ctctcagaat tataatggaa 52740
agaaacagct cctgagtgtt tactatgagc caagggctgt gctaaacact ttaccatatg 52800
atgacatctt tttctcacag gtatcaaaaa acaataggac ataccggata gctacaatct 52860
ttgggcccct gcaaacacaa taatgtgtat tctcttcttc aaatcctaca tattgctaca 52920
aactgtatcc ctgaggcata ttcattgtaa aataaaaaca tataaagtac tacttttgtt 52980
ttttgagatg gagtctcgct ctgtcaccca gactggagtg caatagcatg atcgtggctc 53040
actgcaaccc cctgctcctg ggctcaagtg attctcctga ctcagcctct caagtagctg 53100
ggattacagg cgcacgcccc catgcctggc taatttttgt acttttaata gagaccaggt 53160
ttcaccatgt tggccaggct ggtctcaaac tcctgacctc aagtgatcca cctgcctcgg 53220
ccttccaaag tgctggcatt acagatgtga gccactgcac ccggcccata taaagtacta 53280
ctaatgtaac agggtgctag tccagacagt gaccacacgt ggtgttcatt gaaggctgga 53340
ctaacaactc cagcctctcc gccatcacag agtgatgact gccttccctg aagcaaagct 53400
tctggttcaa ggaaaggcca gtaagtgact gctctttgtt gtatacatgt tagatgatca 53460
ggcctcaaga aaagtataaa gagatctttg tgctctctgg gactcaaaaa gctgcactct 53520
ttgggggaag gatagccagg taaaagtggc ccaggtaaag agggcctggt acacctggtt 53580
ctgcaagatg gtagacacaa aaatgagagc cacatttgga gcttatgtgc ccctaactct 53640
gtacataacc tgcaagatct aattactaac aactggaatc ttggaaacac ctgtagtaca 53700
tccttggcta aggttagccc caacagagag ggctctcctc ttacagagaa ccattacatt 53760
tgtgccttca tcctagagta gaaaaggcat gatcagacta ctaaaaagac atcaggaaag 53820
ggcctgtgac atctgaggga agtggttgcc ctctctggga tgttggttcg ggaagagggg 53880
catggaggag tgcctgcttt agatggtcat tcaggaaccc aggctgatag tgagaggtga 53940
agccagctgg gcttctgggc taggggggac ttggagaact tttgtgtcta gctaaaggat 54000
tgtaaatgca ccaatcagca ctctgtaaaa tggaccaatc agcactctgt aaaatggacc 54060
aatcagcagg atgtgggcag ggccaaataa gggaataaaa gctggccacc agagccagca 54120
gtggcaaact gctcaggtcc ccttccacgc tgtggaagct ttgttctttt gctcttcaca 54180
ataaatcttg ctgctgctca ctctttgggt ctgcactatc tttatgagct gtaacactca 54240
ccgtgagggt ctgtggcttc attcctgaag tcagtgagac cacaaaccca ctgggaggaa 54300
caaacaactc tggacacgcc aactttaaga gctgtaacat tcactgcgaa ggtctgcggc 54360
ttcacctctg aagtcagcga gactatgaac ccactggaag gaagaaactc cagacacatc 54420
tgaacatctg aaggaagaaa ctccagacac accatcttta agagctgtaa cactcactgc 54480
aagggtctgc ggcttcattc ttgaagtcag caagaccaag aacccactgg aaggaaacaa 54540
ttccggacac attttggtga cccagatggg actatcacca agtggtgagt accatcaacc 54600
cctttcactt gttattctgt cctatttttc cttagaattc gggggctaaa tattgggcac 54660
ctgtcagcca gttaaaagcg actagcatgg ctgccagact taagagacta aagacacggg 54720
tgtcagactt tctgggaaag ggctctctaa taacccccaa ctctttggag ttgggagcgt 54780
tggtttgcct ggaaccagct tccacatttc ctgtacttct gggctgagac gagggtcaac 54840
agagaggaaa gccattcagc tctggggtcc cgacagcaag ttggttgacc ctgtggccat 54900
gaacagaact ctcgaagtca tgttgcccaa gcgagactca cccatctatc ctatctatcc 54960
tgactcttgc ttcctgggtc ctaatgcctg gaagacaaaa cttcctcttg tctctgttct 55020
ccaaggctag tcccacttct aaaaaccact ccctgtctct ggtgcttttc tagtttctcc 55080
tataagaatg atttctagta taaactccag gactctattc tcttctttag gcacccgggc 55140
tcaccaatca gaaagccata atttttgccc aaagccccat cttagggggg actatctgga 55200
attttaggat ccctcctcag acaagcaggc ctaacaaaag ctattcctga agctaggata 55260
tggggagcct cagaaatgat atccttccta ttcaagtgag gacaaaaggc atcactcttc 55320
caattctgga aatcccttcc ctccctcagg gtatggccct ccacttcact tttggggcat 55380
aacgtcttta taggacacgg gtaaagtccc aatgctaaca ggagaatgtt taggactcta 55440
acaggttttc aagaatgtgt cggtaagggc cactaaatcc gatttttctc agtcctcttt 55500
gtggtctagg aggacaggta agggtgcagg ttttcaaaaa tgtgttggta agggccacta 55560
aatctgacat tccttggtcc tccttgtggt ctaggaggaa aactagtgtt tctgctgctg 55620
catcagtgag cgcaactatt ccaatcaaca gggtccaggg accattgtgg gttcttgggc 55680
aagaggtgtt tctgctgctg cattggtggg ctcaactatt ccaatcagca gggtccagtg 55740
acctttgcgg gttcttgggt cggggggtgg ggggaacaaa cagaccaaaa ctgggggcag 55800
ttttgtcttt cagatgggaa acactcaggc accaacaggc tcacccttga aatgtatcct 55860
aagccattgg gactaatttg acccgcaaac cctgaaaaag agtggctcat tttattctgc 55920
actatggcct ggtcccaata ttctctctct gatggggaaa aatggccacc tgaaggaagt 55980
ataaattaca atactatcct gcagcttgac cttttctgta agaaggaaag caaatggagt 56040
gaaatacctt atgtccaaac tttcttttca ttaaaggaaa atccacaact atgcaaaact 56100
tacaattcac atcccacaag aggacctctc agcttacccc catatcatag cttccctata 56160
gctccccttc ctattaatga taagcctcct taatctcccc cacccagaag gaaacaagca 56220
aagaaatctc caaaggacca caaaaacccc tgggctatcg gttatgtccc cttcaagctg 56280
tagcggggga ggggaatttg gcccaaccca ggtacatgtc cccttctccc tctctgattt 56340
aaagcagatc aaggcagacc aggggaagct ttcagatgat cctgataggt atacagatgt 56400
cctacagggt ctagggcaaa ccttcaatct cacttggaga gatgtcatgc tattgttaga 56460
tcaaaccctg gcctttaatt taaagaatgt ggctttagcc acagcccgag agtttggaga 56520
tacctggtat cttagtcaag taaatgatag aatgacagct ggggaaaggg acaaagtctc 56580
tcccggtcag caagccatcc ctagtgtgga tccccactgg gacctagact cagatcattg 56640
ggactggagt cgcaaacatc tgttgacctg tgttctagaa agactaagga gaattaggaa 56700
agagcctatg aattattcaa tgatgtccac cataactcag gaaaaggaag aaagtcttgc 56760
cttcccttga gtggctacag ggaggcctta aggaaaatat aactcccctg tcacccaact 56820
cacttcaagg gttaattgat tctaaaagat atgtttatta ctcaatcagc tgcagatatc 56880
aggagaaagc tccaaaagca agcccttggc cctgaacaaa atctggaggc attattaaac 56940
ctggcaacct tggtgttcta taataggggc caagaggagc aggccaaaat ggaaaagcga 57000
gataagagaa aggccacagc cttagtcatg gccctcagac aaacaaacct tggtggttca 57060
gagaggacag aaaatggagc aggccaatca cccagtaggg cttgttgtca gtgtggtttg 57120
caaggacagt ttaaaaaaga ttgtcctatg agaaacaagc tgccccctca cccatgtcca 57180
ctatgctgaa gcaatcactg gaagccacac tgccccaaag gacaaagatt atctgggcca 57240
gaagccccca agcagatgat ccaaccacag gactgagggt gctcagggtt agcgccagct 57300
catgtcatca ccctcactga gccctgggta catttaacca ttgagggcca ggaaattgac 57360
ttcctactgg acactggtgc ggctttctca gtgttaacct cctgtcctgg acagctgtcc 57420
tcaaggtctg ttaccatccg aggaatcctg ggacagccta tatccaggta tttctcccac 57480
ctcctcagtt gtaactggga gactttgcta cagatagtaa gtatgcttac ctaatcctac 57540
atgcccatgc tgcgatatgg aaagaaaggg aattcctaac ttctgggtga acccccatta 57600
aatatcacaa ggaaactatg gagttattgc acacagtgca aaaacccaag gaggtggcgg 57660
tcttacattg ccgaagccat caaaagggga aggagagggg agaactgcag cataagtggc 57720
tggcagaggc agggaaagac aagcagaaag gaaagagaga aagagcagaa agtgagagag 57780
aaagagagat aggaagtgat agcaaagagg gagtccgaaa gaaaagagag aggagagaga 57840
gagggggaaa gacagagaga gacagaggaa gagacagaga gacagaaaga gagaagcaaa 57900
gagaggaaga gacaaagaag gagtcaaaga gagggaaaga gaagtagtaa agaaaaaaca 57960
gtgtacccta ttcctttaaa agccaggtta aatttaaaac ctataattga taattgaagg 58020
ccttttctgt aaccctataa tactccaata ccaccttgtt gtcagtgtaa acaagggtat 58080
agcccaaaag cactgaggcc actgacaacc cgtagccttc ttatcaaaaa tccttaacac 58140
agcaggtttc ctaacaggga atctaaatct taaggtcgga ccagacatag gaggaactgc 58200
cttcaggaca ggatgataga tggttcctcc caggtgatta aggaaaaaga cacaatgggt 58260
attcagtaag tgataaggaa actcttatag aagcagagtt aggaaaattg cctaataagt 58320
ggtctgctca aacgttgaag ctgtttgctg tttgcactca gctaaacctt aaagtactta 58380
cagaatcagg aaggagccat ctataccaat tctaagttaa tatggactga acgaggtttt 58440
attaatagca aagaaaatta aaatctcaaa cttacaaggt tttcaactaa agtaaagttt 58500
gctaaaagtt aacagcgtaa catgtattat cctactacct cacactctct caaaggattt 58560
ctcagacagt ttgcaaaaaa gaacgaaatc tgtccttact ctacaatccc aaatagactc 58620
tttggcagca gtgactctcc aaaaccgctg aggcctagac tctcttactg ctgagaaagg 58680
aagattctgc acttcttagg ggtagagtgt tgtttttata ctaaccagtc agggataata 58740
tgagatacca cccagtgttt acaggaaaag gcttctgaaa tcagacaatg cctttcaaac 58800
tcttatacca acctctggag ttgggcgaca tggcttctcc cctttctagg tcctgtgaca 58860
gccatcttgc taatagtcgc atttgggccc tgtattttta acctcttggt caaatttgtt 58920
tcctctagga tcgaggccat caagctacag atgatcttac aaatgtaacc ccaaatgagc 58980
tcaactaaca acttctgctg aggacccctg gaccgacccg ctggcccttt caatggccta 59040
aagagctccc ctctggagga cactaccact gcagggcccc ttcttcaccc ctatccagca 59100
ggaagtagct acagcggtca tcgccaaatc ccaacagcag ctggggtgtc ctgtttggag 59160
gggggattga gaggtgaagc cagctgggct tctgggtcag gtggggactt ggagaacttt 59220
tgtgtctagc taaaggattg taaatgcacc aatcagcact ctgtgtctag ctaaaggatt 59280
gtaaatgcac caatcagcac tctgtaaaat ggaccaatca gcaggatgtg ggcggggtca 59340
aataagggag taaaaactgg ccacccgagc cagcagtggc aacccactcg ggtccccttc 59400
cacactgtgg aagctttgtt cttttgctct tcacaataaa tcttgctgct gctcattctt 59460
tgtgtccaca ctacctttat gagctgtaac actcactgcg agggtctgtg gcttcattcc 59520
tgaagtcaac agaccacgaa cccactggaa ggaacaaaga actcccgatg tgctgccttt 59580
aagagctgta acactcactg cgaagctctg cagcttcact cctgaagtca gtgagaccac 59640
aaacccacca gaaggaagaa actctggaca cacctgaata tctgaaggaa caaactccag 59700
acacaccatc tttcagagct gtaacactca ccgcaagggt ctgtggcttc attcttgaag 59760
tcagcaagac caagaaccca ccggaaggaa caaattccag acacagtagg aaatctgtat 59820
ttttgatctg tggcttccag ggttactcca gtcattgaag tctccattgc agccttaagg 59880
aaacagagaa tggtttggag gagcacatgt gggaattgtt atggaccagg cttgagatgc 59940
acatagggca tttctgatca aacctagctg gaagcagggc caggaaatat aatctaagga 60000
agacagtttt tgtagacagt agtagtcttt gcatctgaga catgtagatt atcaagcaat 60060
taattagaaa aaatatagcc aggtgcgatg gctcatgcct gtaatcccag cactttggga 60120
ggccaagggg tgtggatcac aaggtcaggc gttcgagacc agcctggcca acatggtgaa 60180
accccgtctc tactaaaaat acaaaaatta gcctggtgtg gtggcacgca tctgtaatcc 60240
cagtactcag gaggctgagg caggggaatc tcttgaactt gggaggcaga ggttgcagtg 60300
agccaagatc acaccacagc actccatcct gggtgacaga gcgagactct gtctcaaaaa 60360
aaaaaaaaaa aaaaggaaag gaaaatataa tcaagaatat tgacaggtaa catttattca 60420
acacttacta tgcaccaggc aatacactaa gtgttttaca tggattaact catttaatct 60480
taacaatagc cctatgaagt cagtgctgtt attatctcca ctttatagat aaggaaactg 60540
aagtacagaa aggtcaagta gagaaatggc catgcttgca ttctcagttt ttgaagcaac 60600
tgttacagga atctggtgtg agaaatgctc taacaagatg tgagtcaggg gttgggaggt 60660
actgagtctg agttgggcag ttggggatgg aaggatggat gaagaacagc ttgacagaga 60720
agctgacact tggcaactct gtgggacctt gaagggttag agggacttca ccaaagaaac 60780
tggtggtcag ggaaacggga gggtcacggc aaggagggaa aggaaactgt accacagcag 60840
agagtctgaa gctactacag tgtagttcag cgtataaaga ataattattt taaggtaaac 60900
ttataacctc atgcaaatat aaaatgaaca cgtgtcaaag atcttattta atttattaat 60960
taatgaggga acctgtaaga tgttacagcc agttcaaagg ataattcaaa taaatccatg 61020
cacatatgta ggcaataagg aatgctgaaa tgaatttaaa agtagatgta aactgattta 61080
tccacagaga aataatcagt tgcatttcac ataacaaaat tcagttgctt ttctacagaa 61140
ggaattgttt gcatcattac caatttttct acaactaaca gaattataaa ataactcaaa 61200
cacaatgaaa ggcagatata acccacaatg gtatgataga tacaatatcc acatccagga 61260
tgtttttttc tcatttcaaa gtctttcaca agttttcctg ataagggagt gtcaataata 61320
ctgtatggca ggcaataaga ctggatggat ggttggggcc aggttttaag gggtaataaa 61380
tgccatgtaa aggtatgtgc atactgtgca acatgtcggg ggaatctcaa attattggta 61440
gagtatgtag gaaacacttg tgggagcttg ttaataaatt caaattccca gacccaactc 61500
ctcaaggggt ctaatacagt aggtttggag taaagcctga aaatctgcaa ttgtgcaaaa 61560
aaaaaaccca ggtgattctg atacactttg agaagcactg gtggaactaa tagtcactga 61620
acgtttttga gcaggggaga aacctgagga cgtctatgtt gcagcagtgg aaacttgatt 61680
agaagtagga gaagatgcat ggtcttaaaa gaatgcaaaa tgatggctaa tatttgagtg 61740
cttatgatgg gccaggggct gtgctaggcg cgtggcacac attcaatacg atggaagcct 61800
gtaccagtca gtattagtgg ggtatcttta agagtgacca gaattaaggg gggttttcac 61860
caaagcctga ggactgagcc tcctcatcct aaattcagac acaatgctgt acctatgcat 61920
ttgcctccag gctgttcctg ggcctccagg gactggccca ggctcctgat aaatagggac 61980
tcccaacaac ataaagcctg gattttggaa cttcctgaat gttactcagg ctttctagta 62040
actgtggaga tctgaataat aacacaattc taagttcccc tactcataaa gctgctcatc 62100
atttagatgg ggtaaagcac ctgaaataca atgagcatca ctattttcat tcatccatga 62160
aatgaacatt ccggggagat cagtaagttg atgtatcacc cttgaacagg gcaaaatgaa 62220
tactcaccag gaatatgtgg tattttaaaa agaaggcaaa gggaagaata gtggggatgg 62280
ggcaaaaact ttaaatagat tcccccaatc atatatggca attgaagata attaaattat 62340
cattttaatt gagtaagtac tcatagagcc ctcactattt gaaaatgaac tgcctcctaa 62400
ttgttattgt gcaaatgtga tacattaaac ttaagctatt ttaataaaac atccattttc 62460
ggaagctgta gtaggttctc ccaggtcaga tttgataagc cataaagaac aaatgccaac 62520
tcctattttt ctatggtgct gggaaataag agagaaatgt gtaattcaaa gcaatcattt 62580
aattttatcc aatagcttga ttctcctctc tcttctagcc ttttagctaa gctgttacca 62640
agtaaccaca ctagttggct tgagtcttac cactgtttcc ctgaccccac agtggagaga 62700
ctgcatctgt taaagagcag ttatgtaacc atggctatgc tgagctggga ttcccaaggc 62760
ttaggttctt tctgtgaatg accttcacca agacacctga ggtctgtgtg gaaccacagg 62820
cttgtcatct ctaaggcaga gttgataatt ccatctgttt cttgagccca cactgagaaa 62880
aagattacat gactgcagtt atttgaatgc ctcatggaaa gacgtcttat aaatattata 62940
attaatgtta tcattaagta atgcttcaat gcagatcttc caagtataaa tatcagctga 63000
gtaagaagtc aatcttccct gaagcaaaat tgaaatttgt aaatgcgatt tctgggagct 63060
tattttgtaa tacatgattc cagagtgtcc ataacacaca caattgtctt ttttccccta 63120
catgggctat ttacaacaaa attggactta taatgtttat ttccagggat gactagaact 63180
ttaataacaa accttgggcc aggcatagtg gctcatgcct ataatcacag cacttcggga 63240
ggctgaggct ggtagattac ttgaggccag gagtttgaga acagcctggc caacatggca 63300
aaaccctgtc tctactaaaa atataaaaat tagccgggtg tggtggcgca tgccagtaat 63360
cccagttact aagtaggctg aggtacgaca atcgctggaa cctgggaggc ggaggttgca 63420
gtgagctgag attgcactac tgcactccag cctgggtgac agagaaagac tctgtctcaa 63480
aaaaaaaaaa aaaaaataat aataataata aaccctgatg aaaggtttct aaaatgtttt 63540
catctaatgg ttttcttgac aattaaattt tctatataat gtcagttcat aaaaaaactg 63600
agaacgacca catgtcatat cgactgctta aaagaaaata cgtatattta caaacatata 63660
cacgatactg tcttttgtct ggttagttta gaggttagat aaactgcagt atgttgtagt 63720
ggacagatca tagaactagg agtcaggatg tctggattcc taggaagcaa tgaataggtt 63780
gcacggtgca gaccagcatc atgagtatcc tcagggagct tgttagaact gcagatcctt 63840
taactcattg aatcagaatc cctaggtgtg gggccctgaa atctgtattt tagcaggctc 63900
tctgggattg tgatgtgcct tagagtttga caaccactgg gtagctgatc ctgacttaga 63960
cttatcaggc atgtgatctt gaacaagtca cataatctca ctgagttcag ttttcttatg 64020
cttaaaatag gcccaataat atctatttca catggactgc tttgaggatt aggcaagaga 64080
tctgtaacag acactgtaga acagtgtctc tggtctacag ctgaccttcc ataaatggta 64140
gttgccttga tctctgctct gccacataat agctggttaa ctatgagcaa gtaatttagt 64200
tcttctcagt ttagtttctt cacctgtaaa agaaggaaaa taactgttat actcaatttc 64260
tgaagtggct ataaaaatca gtttaaatta tgggcattga agctctttgt acactgtata 64320
aggactgtac atctaaggga ttaatgagac caggcttatg attttaagca tggagtaaat 64380
agtaacactg actctgttct atgaaccaca tggaaactct aaagaatatg cacatttgaa 64440
acacaggtat catctgggga aggtgatctg ctcacccaaa ccagttcatg aacatcaatc 64500
tccagtggcg tgctggagct agctgtacca gctcatgagg gccaattgtt tcatttttag 64560
gaattttgtt tgctggttaa aaatagtcat tatttaaaat taaattatgt aaacaataat 64620
attagataaa ataagttaaa ataaaaacaa aggaactaat tatccccaaa ctcttcccca 64680
cctaattatt ttactatctg tgccttggga ttatttacat tgattttatc catatggtga 64740
caatactatt catatataaa tggtgtgctt ctcttcataa ctctacatag cctgatgtca 64800
ggctagtagc ttgaaattgg ccacagtggg agtgtgagca tttgtaccat gaggcttggc 64860
caaggctaca aatccagact tttgtttttc cctcctggag agctgtctgt taaaaattta 64920
ccaacacacc actggtctta cctttgttaa tttaccacag tccaggttct gacctagact 64980
tagaaacctg gatttgtcag caagctgagg atagagccat tatttttaag aaggactcac 65040
attacccaag tgcaaagcct gatatatacc ttcagaatat caatttatta atttacagtg 65100
aagaaagcca ccccagggca ttccccaggg gaaggcaaaa agagctagtt gcacattttg 65160
aatgtttgat gacattaggg taaggtgaca cagaatatcc atttccacaa ctgagatacc 65220
tgctgcctta aggaagggac aggcaagtcc ttgggcagga ccttagattg tcactgtcca 65280
tcttgctcta ggactctcct ttccaggcat gacgatggcc aactctgtcc tcctacccta 65340
ctgatgggat tatcttttct tgacacatgg caatgcctcc aatcagaggc tggtagctat 65400
ttttaatctt cagggcagta tttttcaaag ggaagttcat ggaccatatg catctgtatc 65460
atttagatgt atattaaaaa tgcttagtct tccccagtta tactagatca gaatctctgt 65520
tggtggggcc cacgaatcgg tattttcaac aaatcactag gtaatttctg tatatactat 65580
agtgtgaaga ccactgcttg aaggtttctt tgcatatctc cactaaatat aaaaaatatt 65640
gacttctaga tttaactccc aaagcacttg catttttaag tttctggggg cattatattg 65700
tggtacccct ataccactca cactctagtc aggaggtata ttatggactg aatgtttgtg 65760
tccctccaaa actcatatgt tgaagtctta gcttccaatg tgatagtatt aggagatggt 65820
gccttctgga ggtaaaatca agccctcatg aatgggatta gtgcctttag aaagagagct 65880
ccgtcactgt ctttccatca attgaagatg cagtgagaag ctggtagtct tgcatctgga 65940
agagggccct cacacaacct gatcatgctg gcacctggtc tcagactttc tgcctccaga 66000
actatgagat gataaatttc tgttgttcat accccaccca ggctacaata ttaggttgct 66060
gcaaagtatt tgtgattttt gcctttactt ttcagggcaa aaactgcaat tacttttgtg 66120
ccaacctaat attttgttat agcagcccga actaaggcaa gggagactac atcagacagt 66180
gtagctatgt aagtacaaat gtatccctgt tgaggaaaac taagttctaa ccctgacttc 66240
aggccagtag ccaccttttc aatctctttc atgaagggac cattatcatt atcactggtg 66300
gcaaaaatag aggcacgaga atggaatttg cttttctgtg aaatctcagt gtatacagat 66360
tgaagagcaa gggtttgctt tcatctctaa gaagcaaaag tgagtacgga ctggcacatt 66420
atcagagaaa gaatcattct agctcggtgg gtcttaacca ggagtgaatt tgactccagg 66480
gaacagttgg caatgtctgg agacgttttt atttgttata gctgggggat gagtgggtgg 66540
gttgctactg gcatctagtg ggtggagacc agagatgctg ttaaacatcc cgcaaagcac 66600
aggacagtcc ccgacaacaa agaattatct ggccccaaat atcaatagtg ccaaagttga 66660
gaaacctcat tctagcttcc ttttcccttc tacgttctaa tcaactgttg ttctttcagc 66720
attaggattc atccagcagt ctctttcccc agcaatttgt tgaaattttt ttaaaaatgg 66780
actcatttta gtgtcacaag aaaaaaatac attcacagga aaggatgggt cattttgttt 66840
aatgatgttt tgcctttcac atagcaaaag cttaataaag tatttttaaa taaaatggtg 66900
aatagatcaa aacattaatt tcacatgtgt tttaataaat aacaggaaga tggctatatt 66960
atataaattg ttcttgtata tgtcttgagt ggatcatcaa acacaaacgt atctacatgc 67020
cttttcttgt gaatagatct aataataacg ctcttctaaa aacaaattaa atggatatta 67080
tttgctgaga atgtaatgct tgtgtgaata gaagccagcc ctgaatccaa gcccccagat 67140
ctatttaaag aatttgaaga atgtcagaaa agcacgtggc ttcaaggtta atgtgtaaga 67200
ctcacagaaa cttgaaaaat cactatgact aaaaagaaag tatgagctcc ctgcatgcct 67260
gtaaattgga atgacagcca aaaccagtta attataaaaa cagctaattt aacaggtttt 67320
caaatttgtt tctttctcca agtagcatat agtcaataat ccttaaagag aaagcaaaga 67380
aggggaagca ctgaaccaaa tttgcttttt tgtacctgct cagctcaaat gcagagttct 67440
ctacctggaa attgactgct tccatagttt gatagccaca gagagatggg aacagaagga 67500
gaggtataat cccagacttg attcagctat agagaatgac aatagtgtca gaggccttcc 67560
aaccagagcg actccatctt gaatacgggc tgggtaaaac agggctgaga cctactgggc 67620
tgcattccca ggaggctaag cattctaagt cacaggatga gacaggaggt cagcacaaga 67680
ccttgctgat aaaacaggtt gtaataaaga agccagccaa aacccaccaa aaccaagatg 67740
gccatgagag ttatctgtgg ttggtctcac tgctcattgt atgctaatta taatgtatta 67800
gcatgttaaa agacactccc accagtgcta tgacagttta caggtacatt ggcaacttcc 67860
ggaagttacc ctctatggtc taaaaagggg aggaaccctc acctcccaga attgcccacc 67920
cctttcctgg aaaacttgtg aataattcac ccttgttcag catataatca agaagtaact 67980
gtaagtatcc ttaggccaga agctcaggcc actgctctga atgtggaata gccattcttt 68040
tatcctttac tttcttaata aacttgcttt cactttactg tatggactcc ctgtgaattc 68100
tttcttgcaa gagatccaag aactctctct tggggtctgg atcaggacct ctttccagta 68160
acaatagtag taaggggtca gggagactgg acaaaggagt ttaagaagcc ttagataaag 68220
ggtcctcatc attgtcataa cataaaatca tggactccta gaattttata gctgatagga 68280
ttagaaattt caaaattcaa tttcattaat tttcatctgc gaaaacagat ggccagagag 68340
gccaaacaat ttgttaagga gcactgaggg cagaccacac tggaacgcaa acctcttagc 68400
agagtataca aggcctttga tctcctcagt cagaatgaac tagagctttc cagggtaccc 68460
tttctgactg tttagcatgt ttgccagtct gactaatttt gaagttgctt aaatatctgt 68520
catttccact gtatcataat ctcctcattc atcttcaatc tccaatgcct tgaactcagt 68580
aaatgttagt tgaacaaaag taaattgaac ccagaatttc tgatcataat ctggagcact 68640
ttaaaattgt cagcttactg ggaaacggga taacatgtga tttgtctttg attttttttt 68700
tctcatatgc tttttccacc tatagatgct acacgaatgt ttttaaaatc tgatataaaa 68760
attaaaatta aaaaattaaa aaaagaaaat ttgatacaat gctacattta gagtgttgtg 68820
attagattcc ttaagtgtat catggtgatc tctacatcac gtggtgatca aattgctttg 68880
ggttttaaca cataactgac aaaggcttgg ggacatgtaa gatcccaaat acatttttat 68940
tgattttttt ttcttgtttg tcctctttta aataactttt ttttgttata agaataattc 69000
atgttcagtg gagaaaccat agaaaatagt gacaagtgaa ggaataaatt taaaatgacc 69060
cataattgta ccatacattc tgatttttta aacgctgaac aaattagcct tgggtaagta 69120
ccaggaatag agtgcagcat tgaaagttaa agtttgggga aggatagctg acttaagaaa 69180
ttatctagtt agacattttt tgatggggta attttgcaga tgacattagt gagagaaagg 69240
acttgccact ctcacacagc tagtaggggt gtgggaggat attggaacca agtttcaagt 69300
cttcagtgaa gaatcaaggg agaagttcta aaacctaaca atatccctct ggatggacat 69360
ttattttatt actacaataa gccacacggt gagtcataag gagcatttca ttcttctaat 69420
atgtctctac tgtatttaga atctgataaa gcccctatta gaattcatct ctttaagaat 69480
aaaagaagct gaggaactaa agagagggtt ggaataatcc actaattata tccgttaagc 69540
ttcagttacg ctaataagga atatcacatg actgtggtgt gtgcttgttc tgaacagtaa 69600
agtacatgag gaaagataag attcagggct gaaatgtcct tcagcatatg taggtagtgg 69660
tgatgaaagt cattaaaaga aaaattgatt gaggtatttt agtaaacaaa agaactcacc 69720
acttacccat caggaagtgt attgttaatg cagtgctgtt cagccttctg gaagaaaagg 69780
tttcttcatg cttctctctt tagcctaatt cttatcctgt cacttttcag gcaaaattaa 69840
aaaaaaaaaa agattgaaaa cgatgctcct attttatttg cttcaaaaga aacaggctgt 69900
tgcattgtgc ttggaacagt ttactcttgg ccttgatgta agtgtgaaag gaagcccatg 69960
taattgacta ggcagtatct gaagaagcag gaaatacagt gttaagaaaa tgaacaggca 70020
tgaaaaccat ggctatttga taaaagtaaa taatttctgc agttcacatg ttctcagcat 70080
attttctttg atactgactt gcttaatatg acaatagcag aaccatggta gcttgtaggc 70140
attacttttc ttttaatttc ttttacattt tgaatttacc agcactcaca tttgtattac 70200
ttttgggtta tactgaggat ctataactta tagatcaaat acctgacata tatatgcatt 70260
ctctgaagtc ttagggcaga actagaacat tcttgtgaac atcagtataa gatattaaaa 70320
tggaagtttt gcctaagact gaagacaata aaaatatcat agtctgaaat gaatgccagc 70380
acaccataca ggatttaaat atctatacat atatatgtgt gtgtattata tatatttaat 70440
atatatctgt gtgggatagg aagaggtagg gggaaatcag ttttacaatt attaagtatt 70500
tcacccttga caagagtata tatattggaa atcagttgga gagtattttc aaagataaat 70560
gttagtgtgc tatgaatgaa tccaccccta ccaccactga ggcagggtag gagaggcctg 70620
tgctcctcaa gcatagttgg aaaaggacct caacaagacc acttcaagag tctaatgtgt 70680
ggagactgtt gcttagggag accttatggt ctagcttctg actcacagct aagtcaggga 70740
gacaggttgg ctgctctgat cgtggagtcc aaaagatggc ctgcactgaa aagcctcatg 70800
agtgttgact tagggctagt ctaagaggtc cctggaagaa gaaacactca gtaggagaga 70860
agctggaggt accttcagtg ctgaattgga acctagattc attcccccgt ggagcaaatt 70920
acataggaaa gatgcccagt gatggagagt gggggtgtct ctaacaatta cccacccacc 70980
tgcccccacc cctaagaaaa agaaaatcac atacaaccag tcagctgtaa acatatgccg 71040
agcctagtaa actcagatac taagttacca gggtacctgg caagtaagaa cattcctgat 71100
tcccttccct cctcttcctc tttgccctcc aaccttagtg gctagcaaga tggggagagg 71160
aggagaagct gtaagtgggg aaaaaagagc agctttctct ccttttcagc tgctggattc 71220
tccctcatca taggcctgag ctggggaatc aggaagaagg attcttttta aaactgaagt 71280
aacgttatca tttaatttta aaacatttta aattttgaca atgttgagat tagatatact 71340
aattattaaa ctaagattat gttttgcagc ttgaagtgat aagaaaaacc tcttatctaa 71400
gagcatccag gaaagtcggg ggtttcctga acatcctttt aaatcctttg gaagtcagct 71460
ttcagagagg atttaaagtg tagactgggc cttcagaaac ttggttaatg taggggtttc 71520
ctatgcagac ttggggacta taccttgtgt ggaagagaga aaataagatt atcttacatt 71580
tttcccattc ctttttcaaa aagaaagctc agctagcatg aaagttaaat tcaaaacgta 71640
atgggtatta tttgcatatt caaatctagt gcatatcatg taagtactga attatggtat 71700
tcattatttc aaatgacaag ctggattttt ttttctttcg aatttcacaa attaattttc 71760
cttggaacct tttggtttgg gctttaagag tttaggcttt catcacaaag agaggacagc 71820
cttgaagatt aaagtgtgtg gctcttctca agatgttctt agtccagcaa aggattctat 71880
gcatatttgg gcttccttct gtctcataac ctgtatttct tgatattcta tttatattct 71940
gtaagatttt ttttttaaag gaaaaattct tccatggttg aaggacatgt caaaaataga 72000
ggatacagtt ttatatcaaa ggaagtttca tgatatgact gtagaagctc atttgactta 72060
agacacatca tttcctcatg gaagtgttaa acagatctgt acaataaggt tggcaatctt 72120
tgtgtaaaac agtttttttt ctcctgctct aaagaaagtg tatatttcaa aatgtgaatg 72180
tcagcagtca gaaaatagta tttttttaac ttcgttttca aagtcctcaa aaacctgtac 72240
ctaatcatga attttttttc ccacagattg tttcttcttc tccctcccag aaactttgaa 72300
gtttttctac atgacaccag gacctatgtc tttttttaat tacacagaaa tgaaagaaaa 72360
aaagtgtgtt gtatcgttaa ccaaatatat gaaatcttta agctgtattt ttatttttaa 72420
ctttgttttg caaagaggcc attccctttg gttaaataat ttgttattca cagtttcctt 72480
gtcctcatat tatcaagggg aaaattgtag aaattttaaa ggaagctcta ggcaatgttt 72540
tcatccctga atctttggag agttataaaa acaaacagat tactgaacct gtaagagaac 72600
caatcgtgaa gtcattacat ctaagcataa gcaaaatctc ctcttggatc attaagttat 72660
agaagaaaag aaagcctgca ctttgaaatt taaataaagc ttggtaactt gtaagtcaaa 72720
cacgtaaaat tttacaattc aggaatatcg atagcagttg agtttaatag acttctcaca 72780
ttccaaattt aaagcttcct tctctgtgct aatagagata caatagcagt aggcgtttaa 72840
gaagaatgaa tcaacaattt aaaactataa tgtgtttttt attcatctcc cttattcaca 72900
tatatttgtt ttgttttgag aaggagttct gctctgtcgc ccaggcagga gtgctgtggc 72960
acgatctcag ctcaccgcaa cctctgcctc ccgggttcaa gcgattctct tgcctcagcc 73020
tcctgagtag ctgcgattac aggcgtgcgc cagcaacccc ggctaatttt tgtattttta 73080
gtagagacag ggtttcacca cgttggccag gttggtctcg aacccctgat ctcaagtgat 73140
cagcccgcct cggcctccca aagtgctggg attacaggcg tgagccatca cttctggccc 73200
ttattcgcat acaatttaaa aatcatcaca gaaggtttga aagaaggaag gggcagaaaa 73260
ttacctactt ttcctctccc cagcgatctc cttcaaatct gtgccttttc ctcaggccca 73320
ggcctcaatt tactgagcag tcacacctca cagagggagg tctgggcaat ccactcttgg 73380
tcacaggaaa gccattgacc ctcccacttc ctctcctcca ccttgttctc aactcttgac 73440
tttgggcttt gtttctgttc aagtcctagg aactggtttc ttttatcagg ttaagtgatt 73500
agttctcttt ccctctagtt gctctcactc cctgactctt gccttctgta acaactggag 73560
acaactcttt caaaaccagc tccaagcccc agacttctct ctgggcttta gttcgtaagg 73620
caggtgccct actgagtgag cctagatcag acagaaacat agctgttggc aatgatttag 73680
gtgaatttcc ttccattgtt tttctaatac cttctttttt ttgtaaatat aaccatgcac 73740
atacacacat atttgaatat cctgcctttt tatttaaaat gacaataggt ccgggagtgg 73800
tggctcatgc ctgtaatccc agcactttgg gaggccgagg tgggcaatca cctgaggtca 73860
ggagttcgag accagcctgg ccaacatggt gaaactccat ctctactaaa aatcaaaaat 73920
tagccgggca tggtggcagg ctcccagcta ctcaggaggc tgagatgtga aaatcgcttg 73980
aacccgggag gtagaggttg cagtgagctg agatcttgcc attgcactcc aacctgggca 74040
ataagagcga aactccatct catggaaaaa aaaaaaaaaa agacaggata aacattctag 74100
atagtctcta taatggtcat gattaagaca ataaaatagt ctgaaattgt caatatatat 74160
taataataat ttatttggcc attctgccaa gtagcagaca cctgtcattc tgcccactca 74220
gcacctctct ttcttttagg gaaatgctac ccactctttg catgggttct ggatggaact 74280
gttgatcaca gtgttttcac tccccatttt gcctcaccag aggtagacag aagacccaag 74340
ccaggccagt tacacacaat cttcagataa ttaccgtatt gatcacagta tcaccccact 74400
caaggcttgg ttggagatga gcagaagaga ctaaagctgg gtcattttaa ttaacacctg 74460
taccccaaag aaagactgtc aatgaggctt ttataccgac actcctggtt tccattcttc 74520
ctgatgccat tcatttgacg aactacccaa tctttccaac agtgtctttg gaagaaagat 74580
agtcagaaaa gaagatagag ttgttttctg ttctttgcaa ccaaggaact ctaaatgata 74640
gacttgttgc taggcacttt ggttattttt attatcttga atacttctgt gatatacttc 74700
tttgtgcatg cctgtttgta cggatgtagc tttttatata ttttatataa tttctcagaa 74760
gtggaattac ttagtcaaaa ggtatgaaca tttttctgat tcttaatata aattgtgcaa 74820
atgcttttta agaggattat accagtttac attttgtgtt atatataaca gaaagtacta 74880
ctgaaaaaat attacaaaaa tttgtctctc tgttcaggag gaccttgtaa tagatgataa 74940
agtacttgaa ataggaacat agagcatttt cagtttaaaa taatttcatt gggttattta 75000
cggaatcctt agaattatgg ccagacattt atagatgatc tgtaccaaac ctaggttggt 75060
tacataaatt gcttattcaa ctggcttaaa tctataatag aaagatgaca cttactgaat 75120
gtttaatata cactttgtca ggggctttgt attattctat gacatcttca aaatgaccct 75180
actttcctat tttataagta aggacaggaa ggcttcaaga acatgactaa ttttcccaag 75240
ggctgtacca aagccagaac ccaaatctat aaggctttta aacctgcatt ctaaaactgc 75300
atctcggcca tcttattcct acagaactta aggttagaaa gccagattgg agtcccaatt 75360
tcaccactta gtaaccagac aaacttgagg aattcactca acgtctttga atcttcattt 75420
tctaatcttt aaaactaaaa caataatact tgctctacct atgtcctaag atttcgtgag 75480
gcacatagag atagtgtgga agagtgctgt acagatgtca agtgttagcg tgattactta 75540
gatccctgaa caccatggat gaatgtctct gactgctatt agaggtcata aagaatattg 75600
gggccaggta cattggctta ttcctataat gccagcactt tgggagcctg agacaggagg 75660
atcactcgag gccacgagtt caagaccggc ctgggcaaca tagtgagacc ccttctctac 75720
aaaaaaaaaa gcagccacgt gtagtggcac acacctgtag tcccacatac tcaggagggt 75780
gagttgggag gataacttta gtccaggagt ttcaaggtgc agtgagctgt gattgcacca 75840
ctgtactcta acctggacag cagagtgaga ccctgtctct aaaaaaaaag aaaaaaaaat 75900
aataataata aagaataatg gggccttggg atacccactc ctctctttct gctctgagtt 75960
gtgaagcagt tgagttacat atgcatgtcc aatggatgag gttgaaaata tcaactggat 76020
tggaatgtgg cttacttgcg tggccacaat gagcttcgta acacttcctg acagggtgag 76080
aagacaaact tcctcaccca gtcactggca gagctggaca ctctgtgtct ctcccacaga 76140
acaacctctt actgcatgga ggtggatgaa aaagtcaacc gagaacaggc tactccaaaa 76200
agcagagcac caaaggcacc agctggtcag gtcccccttc ctaagtaaac aatcacgtaa 76260
ttcattcggg acaaagccag agaggtggtg tggagaaaga gagggcagtt tcctcccaag 76320
tttttcctgg aattctttat gggaatatga ggtttagggg aataagactt ccctttaaca 76380
gtgaagaatc cccagctcta ttggtaatag gaaatcgctt acaaggatca tggggagtat 76440
ttcctcagct cgttctgcct cctacttggc tgagtggaat ggaaccatct gtggctgctg 76500
catatgatat tgtcaacttt gtcattccac acccactcct tgacgcccta ccatgtggtc 76560
ataagactcc ctttaaagtg ttcctttaaa aaacaaaatg tgttttgttt ctataaaata 76620
cagctcaatg tcagaaccct tgtcttgttt gctctctgat gtaacccttt cacaatgttt 76680
gggcagctta ttctctctat ttccctgtag ggtcccatcc aggccaaagt gagtgccagc 76740
ctcatttggg cagcagatgc cctgtggaag ggcaggagga gacgagagct aattgtaact 76800
ttgtgattag ctgtcatgga tgcctggtcc tgtcaatagc gctcaataaa gccagaaggc 76860
caagcgttcg cttctgcata ctgattgctg agtcagattt ctcagtgcag aagggctttc 76920
taggcagtca attttagaat attagtcttg gttcttaagt ggttaaaatc cctagctggt 76980
ctttaatctg agcctggaga atttagttat ggctgacatt ctgctgtgat atttttgccc 77040
tcaatatata tgtctttcct ccatctctta gatccctgaa tcatagagat atatatgtta 77100
tataatcaac tgtctccagt ctctaagagt gataagtaca cattgtgtca ggttgagggg 77160
acaggagaac tttcaaaagc ctttcttgcc cctttttcct tctcactgcc tcccactaag 77220
tccagccact tattattcag ctgacactat catcatgacc atgaggtctt ttggggctac 77280
cctggttcgg atccttctgg aggtttgttg cttaactctg tcttcagtcc tatgagctgc 77340
tttttcaata agtttctatt ttggctaaag ttggccagaa tctccttgta accaaagaac 77400
aaataaaata ccagcttgca atgttctatg ttgcttccac caaacttatg cagcacttcc 77460
tatctaatcc acctactagt cttttttttt tttatttttt ttgagacgga gtctcgctct 77520
gttgctcagg atggagtgca atggtgcaat ctcggctcac tgcaacctct gcctcccggg 77580
ttcaagcaat tccccggcct cagcctcctg agtagctggg actacaggtg catgccacca 77640
cgtccggcta atttttgtat tttaggagag agagggtttc accatgttgc ccaggctggt 77700
cacgaactcc tgagctcagg caatccgccc tcctcgggct cccaaagtgc tgggattaca 77760
ggagtgagcc acctcacctg gccccgacct actagtcttt agtgtttgct tccttctatt 77820
gggtaattgt ctgtttatat gcatgtcttg tttcctcaaa taaaatgtgg tcttctcaag 77880
ggtattggcc catgttctat ccatctgtag atatcacagc acctagcagt gtctttcaca 77940
gaggaagtac acaactggca ttattgattc attgctccat tttttccttc tttatcccca 78000
gcatttctca ataatttcaa acatctccat tggagtaccg gagaaagcag gtagctttac 78060
ttgcagctat gtttctatcc ccatagtaac taaaagagga cccagagaaa catgtttaaa 78120
tgctgtcctg ttatcaggac ctcagccttc tgatgctccg tggcttgggg gttattgctt 78180
gatcatctcc tccccaacct acactgtgta cctatgctag tctcttcatg aggactaagc 78240
cccatagtaa aagggctaga taaatagaaa atcattttat gtaattataa gaatgagaat 78300
actgagtatt ctggtgtttg tttaggataa gcacatcttt atttgtatga gaaaaagaaa 78360
aagagagtga aaaatatatt aacgtgcata ttgttcagaa cccttggatt gcaagtgaca 78420
gaaactcaat tcaaaccaac gtaagtcaaa aggaaaatat attggctcat gtaaccttct 78480
cacagagagg gcaggatgga aggggctttg ggaacaagag aattgttctc aaattctagg 78540
aatactagga ttagtccagg atgggtcacc ttcctgtccc tgaggtggtg gtagcgatgg 78600
tagagtctta tgggaggaaa gagtgcatgt taggatgaag gtagggctaa gcaaacaagg 78660
gcaagggcca ctatatcatg ctaaaaatgg ttttttttga tgtcttcctt aatttcacaa 78720
atgcttccaa caaagtagca cacaggaaaa agaacatagg gactctactg gtgggtgctt 78780
ttatcttaag ccttgtactt gcttttcaca gcttactcac tgcttgtacc tgaggccata 78840
tgccctgtaa aagcttctgc agggtttcta ctaagctggg ttccttatat ggctctctcc 78900
catttctgtt gcctcactct agtgatcttt ctcttttcct cacctctggg actggtggct 78960
gtttgtatgg actgccttag ctttgctttg ggttttttcc tggggacaat gtcttcagat 79020
tatcctagac caaataaact acagccactg ggccaggctc ttcctcctcc aactggacca 79080
tgttcccagg gctcttcacc ttagtttagg tcaagcattc ttggcaaaag aaaggcctag 79140
ttaacaatag acattctagc aattgattct ttttgacatg ttgtaagatc tattcacatt 79200
ttgtaattaa agcattcccc tatggaaacc aacacgaact aagctgctcc tggaatgcag 79260
ggtggcctcc tcaatacagg atgttctaga gagctgtatt ttgggcactt aactattctc 79320
cactacttag ggcacagcac tgaaattaac accactaagt ttgtcatgtc catgtagtta 79380
gtctcaggca gtgcagcctc aggagtggaa ctgacctctt atgtgtgtcc agcctttctt 79440
ccttcagaag tcagctgtgt tttctgctga ctctccatag gaacatcagt cctgaatcct 79500
cagaccacca tctggagtag taagtgctcc tgacagtcct agaagttgtc taccgctgga 79560
tctccaaagc gtgtgacaca ccgtgagaga gaaatgagaa agctgggctc ttcaggtaaa 79620
tcttgctttt tcacaagccc cctaatttta ctgcataatt attttgaatt cactgataat 79680
ttctacaatt ttcccataag tcatctacac acaataccct ctcatgcaac acttggcttt 79740
gctaatacat atctattatg agagctgtgc ttcttaagcg taaatgtttt atatgcacta 79800
aggctcttgg cttacatata aaaggggtat tgagcaatgt gatacagaag tcttttctcc 79860
acaggtctca tatgtaaaga attcattaga ttggctgaaa tagactgatc tgtccatttc 79920
tctgctcact tatcataagg aagtcattag ctaaggaaca aaaactacaa tctatgtaat 79980
tagaagaaca agctggtttt gctcaatata aaaataagaa aaagaaacca tgtgaaagtc 80040
aaaatatttg tttaatcagg tcattgagaa tctattaaaa agtatttgaa ttctttatga 80100
tgagaactat cttgactcaa gtggacagtg gtgagctttt tggcctgtgg tccctacgta 80160
gaaaggaggc tttgtcataa agtcttatat ggtacaggtg ccaagttaag tgcccaagct 80220
tgctcttaaa agcatactgg attttg 80246

5

5596

DNA

BAC-F2 contig 1

5
tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca 60
catacgattt aggtgacact atagaaccag atctgatatc gaatgaattc tttcttgcaa 120
gagatccaag aactctctct tggggtctgg atcaggacct ctttccagta acaatagtag 180
taaggggtca gggagactgg acaaaggagt ttaagaagcc ttagataaag ggtcctcatc 240
attgtcataa cataaaatca tggactccta gaattttata gctgatagga ttagaaattt 300
caaaattcaa tttcattaat tttcatctgc gaaaacagat ggccagagag gccaaacaat 360
ttgttaagga gcactgaggg cagaccacac tggaacgcaa acctcttagc agagtataca 420
aggcctttga tctcctcagt cagaatgaac tagagctttc cagggtaccc tttctgactg 480
tttagcatgt ttgccagtct gactaatttt gaagttgctt aaatatctgt catttccact 540
gtatcataat ctcctcattc atcttcaatc tccaatgcct tgaactcagt aaatgttart 600
tgaacaaaag taaattgaac ccagaatttc tgatcataat ctggagcact ttaaaattgt 660
cagcttactg ggaaacggga taacatgtga tttgtctttg attttttttt tctcatatgc 720
tttttccacc tatagatgct acacgaatgt ttttaaaatc tgatataaaa attaaaatta 780
aaaaattaaa aaaagaaaat ttgatacaat gctacattta gagtgttgtg attagattcc 840
ttaagtgtat catggtgatc tctacatcac gtggtgatca aattgctttg ggttttaaca 900
cataactgac aaaggcttgg ggacatgtaa gatcccaaat acatttttat tgattttttt 960
ttctkgtttg tcctctttta aataactttt ttttgttata agaataattc atgttcagtg 1020
gagaaaccat agaaaatagt gacaagtgaa ggaataaatt taaaatgacc cataattgta 1080
ccatacattc tgatttttta aacgctgaac aaattagcct tgggtaagta ccaggaatag 1140
agtgcagcat tgaaagttaa agtttgggga aggatagctg acttaagaaa ttatctagtt 1200
agacattttt tggatggggt aattttgcag atgacattag tgagagaaag gacttgccac 1260
tctcacacag ctagtagggg tgtgggagga tattggaacc aagtttcaag tcttcagtga 1320
agaatcaagg gagaagttct aaaacctaac aatatccctc tggatggaca tttattttat 1380
tactacaata agccacacgg tgagtcataa ggagcatttc attcttctaa tatgtctcta 1440
ctgtatttag aatctgataa agcccctatt agaattcatc tctttaagaa taaaagaagc 1500
tgaggaacta aagagagggt tggaataatc cactaattat atccgttaag cttcagttac 1560
gctaataagg aatatcacat gactgtggtg tgtgcttgtt ctgaacagta aagtacatga 1620
ggaaagataa gattcagggc tgaaatgtcc ttcagcatat gtaggtagtg gtgatgaaag 1680
tcattaaaag aaaaattgat tgaggtattt tagtaaacaa aagaactcac cacttaccca 1740
tcaggaagtg tattgttaat gcagtgctgt tcagccttct ggaagaaaag gtttcttcat 1800
gcttctctct ttagcctaat tcttatcctg tcacttttca ggcaaaatta aaaaaaaaaa 1860
aagattgaaa acgatgctcc tattttattt gcttcaaaag aaacaggctg ttgcattgtg 1920
cttggaacag tttactcttg gccttgatgt aagtgtgaaa ggaagcccat gtaattgact 1980
aggcagtatc tgaagaagca ggaaatacag tgttaagaaa atgaacaggc atgaaaacca 2040
tggctatttg ataaaagtaa ataatttctg cagttcacat gttctcagca tattttcttt 2100
gatactgact tgcttaatat gacaatagca gaaccatggt agcttgtagg cattactttt 2160
cttttaattt cttttacatt ttgaatttac cagcactcac atttgtatta cttttgggtt 2220
atactgagga tctataactt atagatcaaa tacctgacat atatatgcat tctctgaagt 2280
cttagggcag aactagaaca ttcttgtgaa catcagtata agatattaaa atggaagttt 2340
tgcctaagac tgaagacaat aaaaatatca tagtctgaaa tgaatgccag cacaccatac 2400
aggatttaaa tatctataca tatatatgtg tgtgtattat atatatttaa tatatatctg 2460
tgtgggatag gaagaggtag ggggaaatca gttttacaat tattaagtat ttcacccttg 2520
acaagagtat atatattgga aatcagttgg agagtatttt caaagataaa tgttagtgtg 2580
ctatgaatga atccacccct accaccactg aggcagggta ggagaggcct gtgctcctca 2640
agcatagttg gaaaaggacc tcaacaagac cacttcaaga gtctaatgtg tggagactgt 2700
tgcttaggga gaccttatgg tctagcttct gactcacagc taagtcaggg agacaggttg 2760
gctgctctga tcgtggagtc caaaagatgg cctgcactga aaagcctcat gagtgttgac 2820
ttagggctag tctaagaggt ccctggaaga agaaacactc agtaggagag aagctggagg 2880
taccttcagt gctgaattgg aacctagatt cattcccccg tggagcaaat tacataggaa 2940
agatgcccag tgatggagag tgggggtgtc tctaacaatt acccacccac ctgcccccac 3000
ccctaagaaa aagaaaatca catacaacca gtcagctgta aacatatgcc gagcctagta 3060
aactcagata ctaagttacc agggtacctg gcaagtaaga acattcctga ttcccttccc 3120
tcctcttcct ctttgccctc caaccttagt ggctagcaag atggggagag gaggagaagc 3180
tgtaagtggg gaaaaaagag cagctttctc tccttttcag ctgctggatt ctccctcatc 3240
ataggcctga gctggggaat caggaagaag gattcttttt aaaactgaag taacgttatc 3300
atttaatttt aaaacatttt aaattttgac aatgttgaga ttagatatac taattattaa 3360
actaagatta tgttttgcag cttgaagtga taagaaaaac ctcttatcta agagcatcca 3420
ggaaagtcgg gggtttcctg aacatccttt taaatccttt ggaagtcagc tttcagagag 3480
gatttaaagt gtagactggg ccttcagaaa cttggttaat gtaggggttt cctatgcaga 3540
cttggggact ataccttgtg tggaagagag aaaataagat tatcttacat ttttcccatt 3600
cctttttcaa aaagaaagct cagctagcat gaaagttaaa ttcaaaacgt aatgggtatt 3660
atttgcatat tcaaatctag tgcatatcat gtaagtactg aattatggta ttcattattt 3720
caaatgacaa gctggatttt tttttctttc gaatttcaca aattaatttt ccttggaacc 3780
ttttggtttg ggctttaaga gtttaggctt tcatcacaaa gagaggacag ccttgaagat 3840
taaagtgtgt ggctcttctc aagatgttct tagtccagca aaggattcta tgcatatttg 3900
ggcttccttc tgtctcataa cctgtatttc ttgatattct atttatattc tgtaagattt 3960
tttttttaaa ggaaaaattc ttccatggtt gaaggacatg tcaaaaatag aggatacagt 4020
tttatatcaa aggaagtttc atgatatgac tgtagaagct catttgactt aagacacatc 4080
atttcctcat ggaagtgtta aacagatctg tacaataagg ttggcaatct ttgtgtaaaa 4140
cagttttttt tctcctgctc taaagaaagt gtatatttca aaatgtgaat gtcagcagtc 4200
agaaaatagt atttttttaa cttcgttttc aaagtcctca aaaacctgta cctaatcatg 4260
aatttttttt cccacagatt gtttcttctt ctccctccca gaaactttga agtttttcta 4320
catgacacca ggacctatgt ctttttttaa ttacacagaa atgaaagaaa aaaagtgtgt 4380
tgtatcgtta accaaatata tgaaatcttt aagctgtatt tttattttta actttgtttt 4440
gcaaagaggc cattcccttt ggttaaataa tttgttattc acagtttcct tgtcctcata 4500
ttatcaaggg gaaaattgta gaaattttaa aggaagctct aggcaatgtt ttcatccctg 4560
aatctttgga gagttataaa aacaaacaga ttactgaacc tgtaagagaa ccaatcgtga 4620
agtcattaca tctaagcata agcaaaatct cctcttggat cattaagtta tagaagaaaa 4680
gaaagcctgc actttgaaat ttaaataaag cttggtaact tgtaagtcaa acacgtaaaa 4740
ttttacaatt caggaatatc gatagcagtt gagtttaata gacttctcac attccaaatt 4800
taaagcttcc ttctctgtgc taatagagat acaatagcag taggcgttta agaagaatga 4860
atcaacaatt taaaactata atgtgttttt tattcatctc ccttattcac atatatttgt 4920
tttgttttga gaaggagttc tgctctgtcg cccaggcagg agtgctgtgg cacgatctca 4980
gctcaccgca acctctgcct cccgggttca agcgattctc ttgcctcagc ctcctgagta 5040
gctgcgatta caggcgtgcg ccagcaaccc cggctaattt ttgtattttt agtagagaca 5100
gggtttcacc acgttggcca ggttggtctc gaacccctga tctcaagtga tcagcccgcc 5160
tcggcctccc aaagtgctgg gattacaggc gtgagccatc acttctggcc cttattcgca 5220
tacaatttaa aaatcatcac agaaggtttg aaagaaggaa ggggcagaaa attacctact 5280
tttcctctcc ccagcgatct ccttcaaatc tgtgcctttt cctcaggccc aggcctcaat 5340
ttactgagca gtcacacctc acagagggag gtctgggcaa tccactcttg gtcacaggaa 5400
agccattgac cctcccactt cctctcctcc accttgttct caactcttga ctttgggctt 5460
tgtttctgtt caagtcctag gaactggttt cttttatcag gttaagtgat tagttctctt 5520
tccctctagt tgctctcact ccctgactcg ggggatccac tagttctaga gcggccgcca 5580
ccgcgtggac tcacag 5596

6

18443

DNA

BAC-F2 contig 2

6
gagggcggga accccctttc caaaaaaaaa gaaacaaaga caggataaac attctagata 60
gtctctataa tggtcatgat taagacaata aaatagtctg aaattgtcaa tatatattaa 120
taataattta tttggccatt ctgccaagta gcagacacct gtcattctgc ccactcagca 180
cctctctttc ttttagggaa atgctaccca ctctttgcat gggttctgga tggaactgtt 240
gatcacagtg ttttcactcc ccattttgcc tcaccagagg tagacagaag acccaagcca 300
ggccagttac acacaatctt cagataatta ccgtattgat cacagtatca ccccactcaa 360
ggcttggttg gagatgagca gaagagacta aagctgggtc attttaatta acacctgtac 420
cccaaagaaa gactgtcaat gaggctttta taccgacact cctggtttcc attcttcctg 480
atgccattca tttgacgaac tacccaatct ttccaacagt gtctttggaa gaaagatagt 540
cagaaaagaa gatagagttg ttttctgttc tttgcaacca aggaactcta aatgatagac 600
ttgttgctag gcactttggt tatttttatt atcttgaata cttctgtgat atacttcttt 660
gtgcatgcct gtttgtacgg atgtagcttt ttatatattt tatataattt ctcagaagtg 720
gaattactta gtcaaaaggt atgaacattt ttctgattct taatataaat tgtgcaaatg 780
ctttttaaga ggattatacc agtttacatt ttgtgttata tataacagaa agtactactg 840
aaaaaatatt acaaaaattt gtctctctgt tcaggaggac cttgtaatag atgataaagt 900
acttgaaata ggaacataga gcattttcag tttaaaataa tttcattggg ttatttacgg 960
aatccttaga attatggcca gacatttata gatgatctgt accaaaccta ggttggttac 1020
ataaattgct tattcaactg gcttaaatct ataatagaaa gatgacactt actgaatgtt 1080
taatatacac tttgtcaggg gctttgtatt attctatgac atcttcaaaa tgaccctact 1140
ttcctatttt ataagtaagg acaggaaggc ttcaagaaca tgactaattt tcccaagggc 1200
tgtaccaaag ccagaaccca aatctataag gcttttaaac ctgcattcta aaactgcatc 1260
tcggccatct tattcctaca gaacttaagg ttagaaagcc agattggagt cccaatttca 1320
ccacttagta accagacaaa cttgaggaat tcactcaacg tctttgaatc ttcattttct 1380
aatctttaaa actaaaacaa taatacttgc tctacctatg tcctaagatt tcgtgaggca 1440
catagagata gtgtggaaga gtgctgtaca gatgtcaagt gttagcgtga ttacttagat 1500
ccctgaacac catggatgaa tgtctctgac tgctattaga ggtcataaag aatattgggg 1560
ccaggtacat tggcttattc ctataatgcc agcactttgg gagcctgaga caggaggatc 1620
actcgaggcc acgagttcaa gaccggcctg ggcaacatag tgagacccct tctctacaaa 1680
aaaaaaagca gccacgtgta gtggcacaca cctgtagtcc cacatactca ggagggtgag 1740
ttgggaggat aactttagtc caggagtttc aaggtgcagt gagctgtgat tgcaccactg 1800
tactctaacc tggacagcag agtgagaccc tgtctctaaa aaaaaagaaa aaaaaataat 1860
aataataaag aataatgggg ccttgggata cccactcctc tctttctgct ctgagttgtg 1920
aagcagttga gttacatatg catgtccaat ggatgaggtt gaaaatatca actggattgg 1980
aatgtggctt acttgcgtgg ccacaatgag cttcgtaaca cttcctgaca gggtgagaag 2040
acaaacttcc tcacccagtc actggcagag ctggacactc tgtgtctctc ccacagaaca 2100
acctcttact gcatggaggt ggatgaaaaa gtcaaccgag aacaggctac tccaaaaagc 2160
agagcaccaa aggcaccagc tggtcaggtc ccccttccta agtaaacaat cacgtaattc 2220
attcgggaca aagccagaga ggtggtgtgg agaaagagag ggcagtttcc tcccaagttt 2280
ttcctggaat tctttatggg aatatgaggt ttaggggaat aagacttccc tttaacagtg 2340
aagaatcccc agctctattg gtaataggaa atcgcttaca aggatcatgg ggagtatttc 2400
ctcagctcgt tctgcctcct acttggctga gtggaatgga accatctgtg gctgctgcat 2460
atgatattgt caactttgtc attccacacc cactccttga cgccctacca tgtggtcata 2520
agactccctt taaagtgttc ctttaaaaaa caaaatgtgt tttgtttcta taaaatacag 2580
ctcaatgtca gaacccttgt cttgtttgct ctctgatgta accctttcac aatgtttggg 2640
cagcttattc tctctatttc cctgtagggt cccatccagg ccaaagtgag tgccagcctc 2700
atttgggcag cagatgccct gtggaagggc aggaggagac gagagctaat tgtaactttg 2760
tgattagctg tcatggatgc ctggtcctgt caatagcgct caataaagcc agaaggccaa 2820
gcgttcgctt ctgcatactg attgctgagt cagatttctc agtgcagaag ggctttctag 2880
gcagtcaatt ttagaatatt agtcttggtt cttaagtggt taaaatccct agctggtctt 2940
taatctgagc ctggagaatt tagttatggc tgacattctg ctgtgatatt tttgccctca 3000
atatatatgt ctttcctcca tctcttagat ccctgaatca tagagatata tatgttatat 3060
aatcaactgt ctccagtctc taagagtgat aagtacacat tgtgtcaggt tgaggggaca 3120
ggagaacttt caaaagcctt tcttgcccct ttttccttct cactgcctcc cactaagtcc 3180
agccacttat tattcagctg acactatcat catgaccatg aggtcttttg gggctaccct 3240
ggttcggatc cttctggagg tttgttgctt aactctgtct tcagtcctat gagctgcttt 3300
ttcaataagt ttctattttg gctaaagttg gccagaatct ccttgtaacc aaagaacaaa 3360
taaaatacca gcttgcaatg ttctatgttg cttccaccaa acttatgcag cacttcctat 3420
ctaatccacc tactagtctt tttttttttt attttttttg agacggagtc tcgctctgtt 3480
gctcaggatg gagtgcaatg gtgcaatctc ggctcactgc aacctctgcc tcccgggttc 3540
aagcaattcc ccggcctcag cctcctgagt agctgggact acaggtgcat gccaccacgt 3600
ccggctaatt tttgtatttt aggagagaga gggtttcacc atgttgccca ggctggtcac 3660
gaactcctga gctcaggcaa tccgccctcc tcgggctccc aaagtgctgg gattacagga 3720
gtgagccacc tcacctggcc ccgacctact agtctttagt gtttgcttcc ttctattggg 3780
taattgtctg tttatatgca tgtcttgttt cctcaaataa aatgtggtct tctcaagggt 3840
attggcccat gttctatcca tctgtagata tcacagcacc tagcagtgtc tttcacagag 3900
gaagtacaca actggcatta ttgattcatt gctccatttt ttccttcttt atccccagca 3960
tttctcaata atttcaaaca tctccattgg agtaccggag aaagcaggta gctttacttg 4020
cagctatgtt tctatcccca tagtaactaa aagaggaccc agagaaacat gtttaaatgc 4080
tgtcctgtta tcaggacctc agccttctga tgctccgtgg cttgggggtt attgcttgat 4140
catctcctcc ccaacctaca ctgtgtacct atgctagtct cttcatgagg actaagcccc 4200
atagtaaaag ggctagataa atagaaaatc attttatgta attataagaa tgagaatact 4260
gagtattctg gtgtttgttt aggataagca catctttatt tgtatgagaa aaagaaaaag 4320
agagtgaaaa atatattaac gtgcatattg ttcagaaccc ttggattgca agtgacagaa 4380
actcaattca aaccaacgta agtcaaaagg aaaatatatt ggctcatgta accttctcac 4440
agagagggca ggatggaagg ggctttggga acaagagaat tgttctcaaa ttctaggaat 4500
actaggatta gtccaggatg ggtcaccttc ctgtccctga ggtggtggta gcgatggtag 4560
agtcttatgg gaggaaagag tgcatgttag gatgaaggta gggctaagca aacaagggca 4620
agggccacta tatcatgcta aaaatggttt tttttgatgt cttccttaat ttcacaaatg 4680
cttccaacaa agtagcacac aggaaaaaga acatagggac tctactggtg ggtgctttta 4740
tcttaagcct tgtacttgct tttcacagct tactcactgc ttgtacctga ggccatatgc 4800
cctgtaaaag cttctgcagg gtttctacta agctgggttc cttatatggc tctctcccat 4860
ttctgttgcc tcactctagt gatctttctc ttttcctcac ctctgggact ggtggctgtt 4920
tgtatggact gccttagctt tgctttgggt tttttcctgg ggacaatgtc ttcagattat 4980
cctagaccaa ataaactaca gccactgggc caggctcttc ctcctccaac tggaccatgt 5040
tcccagggct cttcacctta gtttaggtca agcattcttg gcaaaagaaa ggcctagtta 5100
acaatagaca ttctagcaat tgattctttt tgacatgttg taagatctat tcacattttg 5160
taattaaagc attcccctat ggaaaccaac acgaactaag ctgctcctgg aatgcagggt 5220
ggcctcctca atacaggatg ttctagagag ctgtattttg ggcacttaac tattctccac 5280
tacttagggc acagcactga aattaacacc actaagtttg tcatgtccat gtagttagtc 5340
tcaggcagtg cagcctcagg agtggaactg acctcttatg tgtgtccagc ctttcttcct 5400
tcagaagtca gctgtgtttt ctgctgactc tccataggaa catcagtcct gaatcctcag 5460
accaccatct ggagtagtaa gtgctcctga cagtcctaga agttgtctac cgctggatct 5520
ccaaagcgtg tgacacaccg tgagagagaa atgagaaagc tgggctcttc aggtaaatct 5580
tgctttttca caagccccct aattttactg cataattatt ttgaattcac tgataatttc 5640
tacaattttc ccataagtca tctacacaca ataccctctc atgcaacact tggctttgct 5700
aatacatatc tattatgaga gctgtgcttc ttaagcgtaa atgttttata tgcactaagg 5760
ctcttggctt acatataaaa ggggtattga gcaatgtgat acagaagtct tttctccaca 5820
ggtctcatat gtaaagaatt cattagattg gctgaaatag actgatctgt ccatttctct 5880
gctcacttat cataaggaag tcattagcta aggaacaaaa actacaatct atgtaattag 5940
aagaacaagc tggttttgct caatataaaa ataagaaaaa gaaaccatgt gaaagtcaaa 6000
atatttgttt aatcaggtca ttgagaatct attaaaaagt atttgaattc tttatgatga 6060
gaactatctt gactcaagtg gacagtggtg agctttttgg cctgtggtcc ctacgtagaa 6120
aggaggcttt gtcataaagt cttatatggt acaggtgcca agttaagtgc ccaagcttgm 6180
tcttaaaagc atactggatt ttgttttaga cttttagtga actgaaggga ataaacaaat 6240
ccctctggga gaacttctcc tccatccttg gtgaagtcat tctgccagaa ttctatctgg 6300
tagttacctt ctccgattca ttaaatgttg tcccatggtc cgacatgggt aatttttctc 6360
tcatttgtga ttagttccac tacaaggaat taaatattca acttcttgcc ttctgggata 6420
tactcagcct tatcacagag ctcctccagg gaaggaactt agattctttg aagaacttcc 6480
ctgctcttac ccaaaccgat tcagttgtta attctgtcca ccttgctcca ttttcagtgc 6540
aggagaaaaa gcatttgtgg caagtctgac cttacaaagg ctcgttaatg ctcaataact 6600
gtgaggacct gctataagtc atgcctttta agaaaaaata cacacatgca cacactcacg 6660
acaagactgc aacacaactg tgatggcagc ttgcatattg aaccagctgt ttccctaaaa 6720
catttgattc ggcatccttt gtagacagta aatgcaaaag acttaggttg gaaaagtgca 6780
ttaggttttg attaacgatt ggatgagggc cagttaaatt tttaaatctg aatgagcttg 6840
ctgactcagg agccttagca gcataatgga cagacagtcc tcaaagcttt cattaaaagg 6900
gtttctggta actgatgtct aragaaatga gttgaaatac aattcactga accactcagc 6960
tttcatctaa aacagaatat gtaatctcaa agaactcaac tggtctcttg aaatattcag 7020
gtaaaattaa atgtaaagaa gctagagctt aaatattttg aggaaaggaa gcctcctgta 7080
gctttgtgac tatatcactt tatccttttg aatgccgtat ttaattatgt taattgcatt 7140
ttaagtatag ctggagtcac cgatctgctg aaaacaaact ctasaatggt ttgtgggagg 7200
tgctcaggat gtatcagaga ctgatttgat ttgcatttta tttttaactt tagttcctct 7260
ctgaactctg ccttctcatg tttgtttttt wtgttgttgt tgcttaatac agtcatgtgc 7320
cacctaatga cagggatatg ttctgagaaa tgcattatta ggtgattttg ccattgtgca 7380
aacatcacag tgtacttaca caaacctaga tggcatagcc tactacacac gtctgctata 7440
tggtagagcc tattgcttcc agactacaaa cctgtatagc atgttactgt actaactact 7500
gtaggcagtt gtaacactgg tatttgtgta tctaaaccta tctaaacata gaaaaggtac 7560
aataaaaata cagtattata atcttatggg accactgcta tatatgcagt ccatcattga 7620
ctgaaacatt atgtggtgca tgactataat aggatcaaac tatgcctttg cagaaatccc 7680
cctggaaagc ctctgaaact accctgatct tagaggcagt tttataaatc acggccaatg 7740
attctcagcc tttgggttgt gccagagatg tgtccgctct ccttttgcaa tgaccctaga 7800
ggtaaaggtg ctctttcttc ttctgcttct catgaaaaaa tgtaaatgtt gtattttagc 7860
ttcttttccc agtctagtaa tatcttgtta aatttacaag attgtagcgg tgcctccaaa 7920
aggggatagc aatagttact ttgaaaatgg gtgagttctt tgcaaccatc tctgagttga 7980
acagttcttg tataatctgt cttcccagtt aggctgtgag ccgcctgaag gcagcaagtg 8040
tatctttcac tcttctctga tctcctcagc cactcttctg ccccacaatt ccaaaaatca 8100
gttaccaagc cattgtaatt ccttttctga aatgtgtagt agactccttt tagggtattt 8160
gcccagttca caaagacccc tgccctcttt ggaaatctgt ccttgcagcc atatatggtt 8220
tttgtttgtt tgtttgtttg agacagagtt tcactctgtc gcccaggctg gagtgcagtg 8280
gtgcgatctc ggctcactgc aagctccccc tcccgggttc acgccattct cctgcctcag 8340
cctcccaagt agctgggact acaggcgcct gccaccatac ccagttaatt tttttgtatt 8400
tttagtagag acgggctttc accatgttag ccaggatggt ctcgatctcc tgacctcgtg 8460
atctgcccgc tttggcctcc caaagtgctg ggattacagg cgtgagccac tgcacccggc 8520
agccatatat gttctatatg actctttctg agacaatagc tgattagaac agtgattaga 8580
actgtgattt ctgagacaat agctgatttc tgagacaata gctgattaga acagttgcca 8640
cgagctggac caatcatatt aatattctct atctctctct tttgctctcg aaatctcaaa 8700
ttgagattca gaaacagcta tgtagtctct gtttgtggct agaactgtaa catatgaacc 8760
cagagctaga gagatgcaat attctatcaa gcagagagag aagcagagga agccggtcgg 8820
cacagacgga atgcagtagc acacagagag aagcagacac tcggagatgt ctgacacctt 8880
tctgcttaga ttccagtcag ttcagaggcc cagacgcatt cctgtctgga agcattctga 8940
tcctgttttg taaatcaaca ataaatccct tgccaccctc tttgcgtgtt agcttaagtt 9000
gtcttgctct taaaaatcta aagagttcta aatgatatga aatgtctgtt atacagaaag 9060
tagaatgaca attgccaggg gctgagagga gagggaaatg gaaaattgct caatggttat 9120
agttttagct ttgcaagagg aaaaagttgt ggatattggt ggcacaacaa tgcgaatata 9180
cttaccacta ctgagctcta tgcttagata cggttaagat ggtaaatttt atgttatgta 9240
tattttatcg ctgtttttaa aaaagtttaa aatagcctgt tgtagtcagc ttccttgtct 9300
tccttactac tgcagccata ttcaggtctc catggcccaa ggtatggaca actgtagtca 9360
ccaaactggt ctccccactt ccaccccttg gaatttggtc cccagcaatc taccctacat 9420
gcatggagca atcaatatta cccataaagc actaacgctg tgctgtactc caaaatgcaa 9480
accttcatgg tgtcccattg aattcaggat caagttcata ctccccagct tgtcatacag 9540
gacccagtga tcctttccaa ccttctgacc tactgattcc cagtaggaag caaaccctag 9600
caagactggt ctgcctcatc ccagaacagt acttactcat gctgtttcct tgccatgatt 9660
accttccttc tcctcaccac atcttatctt tctttcactt gatcttagtc caaatgccga 9720
gaagcaatct tatcttactt tcaaagccca ggttcagacc catcaattct ataaaacatt 9780
tctgaccaca ctagtcctcc atggacattt atttgaattg aacttcttag catttaaata 9840
tacacagttt cttattcatc tgtcttgttc ttctgctagt ttataaattg cttgattata 9900
gaacatgagc ttgataatct ttgatttttc ctggatactg tgttcttgct aggctgttaa 9960
taatgcttgt tgaatgaaat gagaaatgaa gaacggctgc tttaccagtt tgtctcttct 10020
gccaactttt ttacatggat tttacacgtc aactttttta cacaatgatt aaatatacct 10080
aatttgatca tcccaacaac actagtaaat atatatgatc attatcctca tactacagat 10140
gaggaaacac aggcacacat cgtttgtttg tttttttttt tgagacggag tcttgctctg 10200
ttgcccaggc tggagtacag tagcacgatc ttggctcact gcaacctctg ctcctgggtt 10260
caggccatty tcctgcytca gcctcccgag tagctgggac tacaggcatg tgccacaatg 10320
cctggctaat ttttgtactt tcagtagaga tggggtttca ctatgttggc caggctgatc 10380
tcgaactcct gacctgatga tctgcctgct tcggactccc aaagtgctgg gattacaagc 10440
atgaaccact gtgctgggcc aagcacacat agttaaataa cttgcaaaaa aaaaaaaatc 10500
gtatctattt gtaggaggca gagtcgtgat tctgagctga atctatttgg ctcctaagct 10560
tatgcttttt ctacagtatc accacatatc ccatactcta ttgttattgt tggctttatt 10620
gcctgttttt cctgtgaatt ttaaccttcc caaaagcagg aatcttatct cagtatatca 10680
cagagaatca ctaagtatct atagaggaaa ggaaggagag aaggaaagaa gaaaaggaag 10740
aaggaaagga gggaagaaag gaagaaggaa aggagggaag aaaggaagga aggaaggagg 10800
gaaggcaaga gggcaggaag acagaaaaga aggaaggaag aaggaaggaa gggagggagg 10860
aaggaaagaa gggagggagg gaggaacgga taggagggca gaaactctgg aaaggagctt 10920
gtcttactcc taagcttggt aaagatcagt cttgcaaggg gcttgactag aaaacactgg 10980
cttatctcac tgaaccatat tcccaatgtc attgactcct ttcccctggg gagtaattca 11040
accatgtgtt cactgtatgg atcagagttg atgatgaata ttctcttgcc tcagtctctt 11100
ttggccagag ttccttggct tccagcctgc tccttgcttg ttttgaacga ataatatatg 11160
actttccttc ttaactggca aatgctgaac tgtggcctct cttaaccctc aagtctcccg 11220
ataaaaagca aaatattaga ttcgctgacc agcgctactc cttaccccgg ctgatttcac 11280
atgaagagct atatatgggg tggtaacata ggtttaagga tggatgtgca tataactcct 11340
ggataccgtt cctgaaaata tactattggg gattatttct ttggttgaag agtcccttca 11400
ctaccacatg tcagtcccct tacctataaa atgggaacct tagggttgtt ataaggatta 11460
aatgagttaa tgtgtataat gtgcttagca cagtacctgc cactcaatgc tattattgtt 11520
gttgttgtta ttattattgg tagtagtagt agcagtagtt gttgtatgaa gatgcatgat 11580
ttcctgggaa aggtagcaca ttaaggcagg atcagtcatg agttacctca agcagattaa 11640
tttactagcc ctttcatgct atttcccaaa gggatggttt atcaagttga ggaagatgta 11700
gatgtgattt atgatggatt tgaggttagt actgtgtatc caggttgtgt gtgagaagac 11760
aagaaggaac tgagggcaca gctgtactta ggaagaactc tggtttgcaa ggtacataag 11820
ctaattcaga cgagtttaaa ccataggaga ttttgttaca aaggcactag gtaactgcag 11880
ggaccaggga gcagggtgtc cactctcatt ccagattctt ttgaattctg tatattttat 11940
tctctttcca caaacagact ttctatccac ggtggtgatg ataaccaata acatttcctt 12000
cagtctcacc cttgtagctc tgtgaccaaa aatgcaaagc tgctgcttct ccagcttcaa 12060
aatttaataa gaatcacagg gcagaacatt tattggctag gcctgagttg catgtctaac 12120
cttggagaac tcactttgaa taggggaatt cagaactagg attggtggct ccacaaatct 12180
cacaaaaatg gagcaaarta ggaactcatc aaacagaaat caatagatct ccactggctt 12240
tatagtacgt ggttctggga atccagatat tcagagccta ggtgaacctg aacatttccc 12300
tttaggcaga tggaaatcca cgttcttcta gctaaaattt ttccattctc tttgagggga 12360
gtttccatgg agaggctagc tttgtgggag agagtgggaa raaacaactc atgctgtttt 12420
tcattgggga ccattcttat tgctacttta gtccagtcct gcccacggat cacacattat 12480
tccttactct tgttgcttct gggctttttc tttttccttt gcatgctgct tatattccct 12540
tccctaaaag ctactctatt aagagggaga ttaggcaagt aggctggttt gattatgtgc 12600
tggtttaacc cataatcaca tacctcaaaa agaaaatgtc agacacacta taatagctcc 12660
agatacaaaa catgaagtac gaagacctct tcagaaaact gcaggcttgc tactcaccca 12720
cagacaaata gagctgattc tattagaaca gtgaggaaag aacacagtaa agaatggcat 12780
ttaagatcaa ttgtggcaat gtctaatttt gtctgggaag accatggcag tgagggatgc 12840
aaagggatga catcaagttt tcagaacagt gcctatatgt ttaggacgaa gagttaaata 12900
atgagagaaa acaaatgcaa tacaatttca ttggctacct ggttagacct agcatgaact 12960
gtgtctgtga tggtgctatt aatttgtgat ggagacattg gatattgtct ttccctattt 13020
ggtaagagct tgattcaggt agagagaaac aataattatt ttacagtgta caaagcactt 13080
tcttatacga tatattattt tcatcctccc aactagtttg ataggcagta atattattcc 13140
catttcacag agggggaaac ctgggttagg gcccaggaac ttggctggtg agtttggaaa 13200
gcttgaatag caatgattat aatcttggtg cacagaagca gccagtgaaa ttctgaaatg 13260
catatttctg ttctctactt ccagagggtc tgattgagtt agcttgggga agggcctaag 13320
aaatggaatc ttttttattc acaccaggtg attttgaagc atggggtcta ctgagtatgc 13380
ttatgaaaca ttaactttag gtcctaggca ctggcttagt tgactgtgag aaactgaagc 13440
acaaaattgt gtgaccaagt tctttctgag cctcagtttc ctcacctgaa aaatgaatga 13500
tgatgataaa aataactagg ctccatgcca agtgatttac atatttcccc tcaaatcatc 13560
tttcttacaa acctaggagt tcggaggcat tgttgttcct atgctatggg actcaaaccc 13620
aaatcatttc tactcactct tcctttcata attgtcagga agattagaca tagaaagtat 13680
ctagcacata ttcctgatgt tgaaggaata gcagcagctg ttataactac tactaaaact 13740
gacaatactg accatacagc caccactaaa atgytggggt tgaattcaga taatctctaa 13800
ggttcttccc agctccacca taccctgatt tcagcatttc aaatatatgc tgtatttgtg 13860
gggggggttc ctagaaagag tgtggcagta actgaactca actatacaaa agaccgaatt 13920
cttcctttag ttggagattt attgattttt gtaagtgagt ttatagacaa aaacgaggaa 13980
gatacagaga aaaaagagaa gaattactgt gctttgatag tagggctatg ggtgattatt 14040
ttatttttaa aattttattt tttatacatt aatgtggttt ctataacaaa cacaaattta 14100
gaataaaagt aagatatttc tcttgtgctt ccaatttacc atatacttct taaatgtatt 14160
tgtatcataa tcatcagctg taagtttact attaaaaaaa atcaacaaaa gaacaatatc 14220
agagctaaag gacttcaggc ctgatgaacc taagtctagt ttctgtgctc actagccttg 14280
gcttatccca aaatattaaa agtaaaatat gatccaatct gcatctcttg cacatgtcat 14340
gttttgtaaa tagaaagttc ttggaacaat ctgtaacatc gttgaagtac ttcattcaat 14400
tcttgggcat taaattttat cttctgttcc tgcctcatat cattaaacag taccttcacc 14460
tacattgcag tcaactatgg aggactaatg ctctattttt tttatgttga acatgaagca 14520
taaacatgta cagctctgaa cctgagtttt ccttgcttta gaaataagag gtgttgatga 14580
aagaggaaat ccctgagact ctgtaaacct wacctgcagg tatgagaata caatctgtgt 14640
ttwatttatk gtattcttwa gcaaaattat agtaaaatta gtatttttct tttcatttgc 14700
tctcgaatta tcctttagta acagagtgaa cttgtatgtc catattttgg gtttaaagaa 14760
catggttact gtagcaaaga aggggctagc ccatgtatta aggtcctgga ttatactgtt 14820
gctcacagga gagcatgggt ttgaagatga ggctgcatag taaagtaggt aaaagtttgg 14880
accttggggc caaactgcct aagctcaaat catggtcctg ccagtactct ctgttcgacc 14940
tttagcaagt tacttaatcc ttgtagacct ctgatttggt ctcttcaaaa tagggatagc 15000
aataatgcct gtcttataga gacattgtga ggattcaatg aattgatatt tgtagaagaa 15060
tattgagttg gttttgctag aagatattaa gtgcgcagtc tttctaaaat aactaaatgc 15120
tacaaaaagc aaaatagcca ttctgcaaag agcagtgatt gaagcaggaa aaatgcctgc 15180
cttcataaag cttacattat aaggagagaa aaataagcaa aacaaactac gtggtatata 15240
tgtaaaataa aaataaagag ggggaagcat ggggtggggc agatattgca gttataaata 15300
gaatggtcat tggaggcttt attgaaaagg ggacatttga gcaaagtctt caagggggta 15360
tggaagtgag ccatgtgagt attttggtgt agggaaggaa aaacatcctt ctaccctctt 15420
aggtttggtg gctaacctaa gaattaaaac aacatagatt aacaagagaa aagcatgcac 15480
atttatttaa tgtttttatg tatacatggg agtcctcaga gaaaaatgaa gacccaaaga 15540
agactttatg ccccaaagct tatatacatt ttttacacaa agaatgataa actgtggaga 15600
tgtgacaaga caaaaggcct tgggctagaa gcagtaaatt gtgggagtaa gggatataca 15660
ggcgaaacta gtggaaaatg aggatgattt tagttttttt ttacaggtcc atttcgatga 15720
taactccagt catctctggt gatactattc ttctcttcct ggcacaagga gggcaccttt 15780
ctcatgggaa attttatgac ctgctttttg gtagaaaggg gaagtctgag agctcttcct 15840
gcccctagtg tttctcaagc gccttcagct caaaataatc attatgccaa agtggcatat 15900
tttgaggtgg catgttctga gccatttcat ggggtaagga tattccaggc tgaaggaact 15960
gggaatgcaa aggcccttag acaggaacat gcctggtata ttcaagagac atctgggaag 16020
ccaaggtaat gaatgacagc agagcatgag ggtgtgggtg gcaggagatg aggagatggt 16080
acaggaggca caaatcaggc agcatgttat tgatcaccgg cagagctcca ggtttcattc 16140
cattctgagt gacatgaacg gccatcaaag gtgtttgagt agaggagtga ctgtgtttag 16200
aatggactgc aggggaataa gggtagaagc gggaagacca gttagaaact gttagagatg 16260
atagtggctt agacctgagt gacagcagta gaataggtaa gagatggatt atgagtgtgt 16320
ctggctgatt cactcttata tcccctatgc taaggcatca tgcttggcac atagtaggga 16380
ctcaataaat acttgcagag cgaatgaata aatgggagtt caacttgggt aaggcaactt 16440
ctctaaggct ctgtttcctc atctctaaaa tgagggtaag aaaaatatta atagatctac 16500
ctccaacggt tattgtggag attaaatgag gtcattccca tgcattgctt agcatagtaa 16560
ctgaaacata agatagggct aagatgtata catacacata aatataaagc atttttgcaa 16620
gagtttacct ttggagacat ggaggaaggt agacttttat tcttcatttt atgaactaaa 16680
agcaaaagaa gaaaacaagt gttgaaatta tgagtcattt tcaagttctt tttgtacttt 16740
tcactaccat ttggaatttt cctataatga atatgcgagg caaagacaga aatgaaagga 16800
taagatcact cagaatttca ggtttttata aagcatcaga aatgtaagac ttttttctgc 16860
tactgcatgg cccatttctc tgactctttg aatgtgggta ttattctcat ctttctccct 16920
cctcttctct ttttggttaa aagtaaagag agcttttgaa gctattatgg aacaagaaca 16980
acagcctagt tcatcctcac attttggagc ctcttattcc ttccaaagaa caaacacatc 17040
tatttagtgg ctaagagtct cttgagctga aaccattcat caccataact acattcaaac 17100
tgtctgaggt atacattata actaagaaaa tggggttcct cattggaatt tacaaactaa 17160
atattcaaag aagggttctg atgcttttaa aataggggcg ccaccaaaag gtaaagtaag 17220
acatgtggtt gaagacacag gaaagggcag aggtcaccag aaaagttggt tgtcacgcct 17280
gatcttaggg cctcataaag aaataattat ggcagaatga gccctaagaa gcaagcactt 17340
tagcatggct ctccctggac aaagtggaga ggcccttcca ccctaactta tcctattgtc 17400
ctggtcttca gtctttcctg tctgtttgcc tttcctggtg ttaatatact tgttcctaag 17460
gttttcaccc tgctgacttt tagctcttct tgctaagatt cctggctgta cattagaaaa 17520
ctcctgagca actaaacaca aaaaaatatt tggcaggggg atagggggtg cttctaggcc 17580
ctaactaaga cctgttaaat tagagtctct ttcgggtggc tcctgggcat tggggttttt 17640
ttgtcctttt tttttttttt tttaaatcta aagcttccca gttgattcca atatgtagcc 17700
agaattgaga ccagaaagct gttaataccc aagtagtata ctaatattaa taatgatcat 17760
aatagattaa taactaacat tgaatgaact ttaaatgtgt tagctgattt aattctcaat 17820
gactctgagg cagttactat tattattaat gtaccccttc tacagatgaa gaattcaaga 17880
taccaaaaat ctacataatt tggcaaacaa gtaaatgcta aagttggaat tcaaacacag 17940
gtagtttagt gtccgagccc acactcttca ccaccacact ggtggattgc ccacctgcaa 18000
tgttaaaaat cgcagaggat agtgatgata ctgcagacac actgcctgca ttttatctcc 18060
tccttgttag gctgagccat tcatacctca gtggtccaca ccttaaaggc aggatataaa 18120
ggtaaatata tgtaccttct ctgatatgaa ctagagactc catcccttct ttttaagtaa 18180
tgtaaatgat taaccagctt tctgttattc ctttcagaat ctcattcata gaataaattc 18240
ctggcataaa ttagtatcat aagttttcta ttattgctca ttaatcagta tgtgatgtaa 18300
gatcaagcag taagagttcc ccccaacccc aaagaatggt ctttctgttt gtgacaaatt 18360
attcttggca atgtaattag ccagttgggt tattgagggg gatccactag ttctagagcg 18420
gccgccaccg cggtggacta gat 18443

7

11811

DNA

BAC-F2 contig 3

7
cctgttaaag tttaccttgt atcttaaaac ttgccctaac cggattaatt ttctggccaa 60
atagggaggc tgaatgaaag tttcacataa accttagata ctcctaatta actgtttttt 120
atgtctgttt ttctaggaca catgttcaaa gagcataatt aactttttaa aagaagctag 180
taagtactga aatagttttt taagtttttt ctacaagaat agaggaagaa aggaaacatg 240
gaattctgaa gggctactta gcaagctgct tatggcataa tctggggtgg gggtgcatag 300
taaaggattt gcattttact gagaccgata catgtcaagg gaatggtatt taaaattagt 360
gatatgtgtt gatttttcaa ggactatagc ccatcaacta caataggctc caaaaaattc 420
tggtgaaatt agcttcttgg agccttccag tttacctact atgttattcc cactataaaa 480
tattctcaac ttttggggtt ttagccactt aagtttttta ttttctctaa tgtctctagt 540
atctgcttta gtttcctgtc aatgctagac tctgtggttc agcagttcat ccattctctt 600
cccagtactc aacctcgttg cttatagttt cattacattc atctagcaaa accttaattc 660
tgtatgtttg ccataccatt agtgcttaga gcattttttc agaaaagaat cctggaaaaa 720
tggatcttat ctcacctggg ccctcaggac tgctgggctg cctggtgtca gcacttcccg 780
ccattttcta tagcaccagt attattctta atactttaaa aaaccaccag gcacggtggc 840
tcacgcctgg aatcccagca ctttgggagg ccaaggtggg cggatcacaa ggtcaggaga 900
tcaagaccat cctggctaac acggtgaaac cctgtctgta ctaaaaatag aaaaaaatta 960
gctgggcgtg gtggcatgca cctgtagtcc cagctgctgg ggaggctgag gcaggagaat 1020
ggcgtgaacc cgggaggcgg agcttgcagt gagccgagat tgcaccactg cactccagcc 1080
tgggtgacag agcgagactc cgtctcaaaa aaaaaaagta aataaaaata aaaaaccata 1140
tcccactatc tcccccttct ctctttgcct gtgatcttgc tgcatactta tggggaaatc 1200
tttaagatgt cagatttcag ttctctcact tttctacaac ttctcccact tttgcctttc 1260
ttatgtacct tcccttcctt cccatctgat tccttatcag tatttacaca tgattagttc 1320
ttgcctaacc taatagaccc tttcttgagt gcaaatcagt ggctattttt gctagggtat 1380
aaaaattacc tatctaatca ccttgacaaa gttaccctgt tatttccaat aacttacttc 1440
ctatggattc ttgtagattt tctttttttt ttttttaatt tttttatttt cagatgtttt 1500
ctcgctttgt caccatgcct ggcctaaatt ctcgtaggtt ttctatgtaa acaatcagat 1560
tttctgcaag tattagtctc ctttctaatt gttataattt taatttcttt ttctttttaa 1620
aatttttcgt agagacaagg ttttgctatg ttgtccagcc tggtcttgaa ctcctgggct 1680
caagcaatcc tcccatctca gcctcccaaa gtgccattac agtggcatga gccactgtgc 1740
ctggccaaat ttcttttctt gttgcgaagg cagacttttc atacaatact gaatagaagt 1800
gatagtagat tactttattt ctgattttca aaggaatgct ttccgtttct ctctgttgaa 1860
gataattgcg tattgttttt ttttttaaat agtaactttt atcaggttaa ggaaggtttc 1920
ttctatttct atttaaaagg attttttaaa atcttgaatt catatgtttt tatctaatgc 1980
attttctaca tcagttgaaa tggttgtatg aactctttta atatgggtga attatattta 2040
tagattttat gttaaaatat ccttgtatat cttggataaa ctcaactgga tcatgattta 2100
tcttttttat atgctagatt caatttgttg atactttgtt atgatttttg aatatatatt 2160
attgtgtaaa agtgagcctg tgattttctt tcttgtaatg tttctgtcca gttttggtgc 2220
ctggttttgc tctctcctta gaatgagctg ggaactagtc actcttgttt tctcacctat 2280
aatagcatct gggtccagtg ttttttatgt gggacaaatt tgaacttgtg gtcaacctct 2340
ttaattgtaa gaatattcag gtcttttgtt cttcctgggc tagtttttta ttctttttct 2400
agagattcgt tcatttttct tagttttatt tgcctataat tgtggataat ctgtttttta 2460
tctgctactt ctgtaattat ttccacattt gatttataat attaacttgt gggccaggcg 2520
tcgtggctca cacctgtaat cccagcactt tgggaggccg aggcgggcgg atcacgaggt 2580
caagagatcg agaccatcct ggcccatggt gaaaccccgt ctctactaaa aatacaaaga 2640
aaaaaattag ccgggcgtgg tggcaggcac ctgtagtccc agctactcag aaggctgagg 2700
caggagaatg gcgtgaaccc aggaggcgga ggttgcagtg agccgagatc gcaccactgc 2760
actccagcct gggcgacaga gcgagactcc atctcaaaaa aaaaaaaaat ttacttgtgt 2820
cttctctttt tacctgtttg ttaatttatc aaataactac ttttggcttt gtttcatttt 2880
tattatacaa taaaatgaaa ttcttttcat tgtatttctt ttcattgatt attcctataa 2940
ttcttaaaca actttataat tgatgtaaca ataacctgta cacatttaaa gtgtaaaatt 3000
tattacattt tgatccatgt atatagcagg gaaatatcac cacaacaaga gtgtgaacat 3060
ataatctctc cccaaagttt tcttgtgtct tttataatca ctgcctcttg cccctgccca 3120
ctccctcatc cttaagcaac cattggtctg ttttctgcca ctatagatta gattgtattt 3180
tctagagttt tatacaagtg aaatcatgta gtatagtatt aaccatgtgt ttgtttgttt 3240
gtttgtttct ttctttcttt cttttttttt tagacggagt ctcgctttgt cacccaggct 3300
aaagtgcagt ggggcgatct cggcttactg ccagctccga ctccggggtt cacaccattc 3360
tcctacctct gcctcccgag tagctgggac tccaggcgtg cccgccacca cgcccagcta 3420
gtttttgtat ttttagtaga gacggggttt caccatgtta gccaggatgg tctcgatctc 3480
ctgacctcgt gatccgccca cctcagcctc ccaaagcgct gggattacag gcaggagcca 3540
ctgcgcccag caactatgtg tttctgatcc tttgtcaggg ctagccaatt cctagagaca 3600
gtgaataact cactcataat ctagctgcct cctttatgtc gctctcatag gactttgaca 3660
cctctctgct acaatccacc tgccctgttc atttcaagat caggtaccag gaaactcggg 3720
acatccctat gctgcagaac tcactgaaat tattcaaact agccagtcct aaacatgctt 3780
accctgcctt gcccattcct tccgctgaaa ccacataaag gctcttgccc atgttttcat 3840
cccattccat tgacctcctt actgacccta gctagtgctt cctcatgtgg cccctgcatg 3900
gcatggtgtg caccttcctc ttcggaactg cgagtaactg tcttgtcagc ggcaatcatc 3960
ttgtgatctg ttggcctcat catatttgaa taacaataaa atctgtttta aggctgggcg 4020
cggtggctca tgcctgtaat cccagcactt tgggaggcca aggcaggcgg atcacgaggt 4080
caagagattg aggtgaaacc ccctctctac taaaagtaga aaaattagct gggcatggtg 4140
gtgcgtgcct gtaatcccag ctactcagga gactgaggca gggaatctct tgaacccagg 4200
aggcagaggt tgcggtgagc caagattgca ccacggcact ccagcctggt gacagagcga 4260
gactccatct caaaaaaaga aaaaaaaaaa actgtcaaat gatactccaa aatggttgta 4320
ccattttata tttgcaacaa caatgtctga gggtactgat tgctccatat ccttgacagc 4380
acttggtata gctgatcttt taattttagt cactttagtg ggcatatact ggtattttat 4440
gttttacttt ttattttcct aatgattaat agtttgcagc atctttcatg tgcttatttc 4500
cctttcatat atcttctttg ataaaaatat ctgttcaaat attttgccca ttattttgtt 4560
ggaatactta ttttcttact gttgagcttt gagagttctt tatatatctg gataccaatc 4620
ctttgtcaga tatatttttt gcaaaatttt ttcccagcct gtgatttagt ttgttattct 4680
catgtctttt aaaaaaaatt gtagttaaaa tatacacata atacaaaatt taacatttta 4740
actctttgta agtatacagt tttgtggtat taagcatagt cacattgttg tgcaaccatc 4800
accgccatcc atctctggaa ctttttcatc ctccctgact gaaattctgt acccatttaa 4860
acactaactt ctcattcccc cttactccag cccctggcaa ccatcgttct gttttccttc 4920
tctatgagtt tgactgctct aagtacttca tataagtgga gtcatacaat attttcattt 4980
tgtgactggc ttattagtat aatgtcttca agtttcatcc atgtggtagc atgtgtcaga 5040
atttccttcc tttttaaggc taacattcca tcctatgtat ataccacatt ttatccattc 5100
atctgttgat ggacatttaa gttgcttcct ccttttggct attgtgaata atgctgctgt 5160
gaatgttgtt gtataaatat ctgttcgagt tcctgctttc aattcttttg agtatgttcc 5220
caaaagtaga attgctgggt catatgttaa tactgtattt agttttttga ggaattgcca 5280
tactgatttc tatagtagtg gtaccattta cattccaacc agcagtgttc agggttccaa 5340
tttgttaaca ttcttgccaa cccttgttgt tttctggatt ttttttattt tggggttttt 5400
tattttattt atttattttt tttttgaggc agagtctcac tctgtcaccc aggctgaagt 5460
gtagtggcgc aatctcggct cactgcaacc tctgcccccc gggttcaagc gattctcctg 5520
cctcagcctc cgagtagctg ggactacagg cgcgcgttac cacgcctggc taattttttg 5580
tatttttagt agaggtgggg tttcactgtg ttaatcagga tggtctcgat ctccggacct 5640
tgtgattcac ccgcctcagc ctcccgaagt gctgggatta caggcgtgag cactatgcct 5700
ggccattttt tatttttaaa caatagccat cctaatgggt atgaaatagg ttttttggtg 5760
ttttgttttt tttttttgag acagaatctt gctgtgttgc cctggctgga gtttagtgac 5820
gtgatctcgg ctcacctcaa cctccgtctc ctgggttcaa gcacttctcc tgcctcagac 5880
ttccaagtgg ctgggactac aggcgcccgc caccacaccc agctagtttt tgtattttta 5940
gtagagatgg ggtttcactg tgttggccag gctggtccac gatccatcca ccttggcctc 6000
ccaaagtgtt gggattacag gggtgagcca ccatgcacag ccagggtttt gttttgtttt 6060
gtttttacta tttttttttt tttttagaga caagctgtct cccaagctgt agtgcagtgg 6120
caccattcgt atctcactgt aacctcaaaa tcctggaccc aagcaatcct cctgcctcag 6180
ccttccatgt agctacctct acagggaatt gcccccatac cccgggaaat tttttttttt 6240
tttttttttt gagagttttg ctcttgttgc ccaggctgga gtgcaatggc atgatcttgg 6300
ctcactgcaa cctcctcttc ctgggttcaa gtgattttcc tgcctcagcc tcctgagtag 6360
ctgggattac aggcgcccgc caccacgcct ggctaatttt ttgtattttt agtagagatg 6420
gggtttcacc atgttggcca ggctgggctc gaactcctga cctcaggtga tccacccacc 6480
ttgacctccc aaagggctgg gattacaggc gtgcgccacc acacctggcc cccagctaac 6540
ttttaaatgt attttgtaga gatgaggtct cactgtgttg gccaggctgg tcttgaactt 6600
ctgagctcaa gtcattctcc cacctcggcc tcccaaagtg ctgggattac aggcatgagc 6660
caccacacct ggcccctttg cccattttaa aaattaggtt gtttttgttg ttgttgagtt 6720
gtaggagctc tttgtatatt ctgcatttcg gttccttatt ggatatgtga ttggcataca 6780
ttttttccca tccatggatt gctttttcat tctgttatag tatccttgat tcacagaagt 6840
ttttaatatt gatgaggtcc tgcttagtct gtgttttgtt ttgttgcttg tgcttttggt 6900
gttatatcca agaaattttt gccaaatcca aagtcatgaa gctttgccct ctgtttcctt 6960
ctgagtttta tagttttagg acttaaattt aggttttcga cccattttta gttaattttt 7020
gcaagtggta taagggaggg gtccagcgtt attgtttcac gtgtagatat acagttttct 7080
gagtaccatt tgatgaaaag gctgtccatt gaattgcttt tgcaactttt atttgggcat 7140
atttatgtga gtctgttact ggttctatat tttactccat tgatctatgt gtctattcct 7200
ctgctaatac tgtcttaaat atggtagcta tatagtaagc cttaacactg agtagataga 7260
tttctcccct ttttttgttc tttttcaaaa ttgtcactgg tttgttttta ttttttactt 7320
tatgcagata atctgtacta tactttggtt tcatgtatca agtagtttgt tccaagttgt 7380
gctttaagca gaacaaataa attttcatat tgttctttgt gttaatctgc aatataaacc 7440
tataccaaat tctattttgt gtatttgttt attgtagtaa tctgactgac tcttttgcct 7500
ccagactcat ctctttcaag gtccccaact gaatcttgtt ttaggtggaa cttagaagca 7560
gtagaagtta agaatctatt tcacagcctt agtagtctag tttcattctc tatataatgt 7620
tgtctatgca agtgagctgc tctccagtgc cttagtttca ctaatgttgg ggaaggtctc 7680
ttctcttgtt ttggacttct ctatcacatt gcctttctca agagaagaca tataatgaaa 7740
gttgatatct ggtgttctag gacttcttca gaagcttgcc agtttttcaa gctgatttct 7800
ctcactggca actcttcaga gtgctgttcc tactccaccc tcccctggtg gtatgtatca 7860
gttttctact catcagcacc cacctactcc tgcctactgt gtttctcaga tgtctgctgc 7920
ctggctagct cattgctgct tttgtcactc atagagctgt cttcttccct ttttttggct 7980
ttctgcctga cttccagggc agctgctctg tcattgcctg tctgccattc tgtctttttt 8040
ccccctaccc cccacagata caacatctac tctaatacca cacattctcc atgttcaaac 8100
taacctcatc actttcccca ccacattccc caaaactggt catcctccag cttatagcat 8160
tgcagttcac tgaagttaga catctgggcc ttgcttacct ccaacatctc attagccttc 8220
gattctaccc ctataaatcc tcttctcagt ctcctttaga tattcctgcc ctgctgtgag 8280
atccatctgg tttattggct agattacttc agaaagcttc agtcagtgac cctccttact 8340
tcaaacccca ccagttgatc cttcactctg ccatcagtca ttgcttctaa aatctaaatt 8400
gttccattta accttgctgt gataaaacct ttggtagttc ttcagtgtgt tcagtggtaa 8460
gttaaaactt tcactgtaat gtacaggccc cttcatgata tgatcgctgc ctcctcgagc 8520
ctcattgtgt gcatttcccc gccccaccct ttcctcaccc accctagtct ttcatgtctg 8580
ccatttttac attcatttag cagatattta ttgaagcccc ctgtgatgtc cttacctagg 8640
tctttcttgt tgccaggacc agacaggctt tttcaagctt ccaagtcatc tcagtttgaa 8700
agactatgtc tgacccttgt cttggccaat tactctttat ccttccaagt tcaatgattg 8760
tcccactgca ctccaaccag agtgagagag caagaccctg tctcagtaaa taaaaataaa 8820
taaataaata aataaataaa taaataaatc agccataatt tatttaatca tgtctctctc 8880
ccccattgat agacgttaag ggtatttcca gtattcttct cttgaaaaca atgctacatt 8940
gaataacctt gtacatgggt cactttgaaa gtatggatat gtatccgtgg aataagtttc 9000
cagaagtgga attgtgtcag aggggttgtg catttgtaat tctgatgaat atttatagat 9060
tatatgagag tacctgttta ctcaaactct tgccaatgca gcattatcaa agttttttat 9120
gttcgccagt gtgatagatt aaaaaatggt atctcagcca ggcgcagtgg ctcacgcctg 9180
taatcccagc actttgggag gctgaggcgg gcagatcacg gggtcaggag atcgagacca 9240
tcctggccaa cacagtgaaa ccctgtctct actaaaaata caaaaaatta tccaggcgtg 9300
gtggcgggca cctgtagtcc cagctactcg gaaggctgag gcaggagaat ggcatgaacc 9360
tgggaggcgg agcttgcact gagccgagat cgcgccacaa cattcgagcc tgggcgacag 9420
agcgagactc cgtctcaaat aataaaaaaa aaagatggta tctcagcatt gatttctttg 9480
atcatcagtg aggttgagca tcttttcata gatttaagag aactgtatgg ttttttgtga 9540
gttatgtttc atatcgttta cccattttac ttttaggctg gaagcagctg ttttagtgga 9600
atggtggaac aagaagccag attgccatgg agagacaact ctttctagag atttggctat 9660
gaagcagagt agagacaatg atagctgaag gattgatgta gatgcaaaga aatttttcat 9720
cttctttgaa aacttaattg tgttaaaaac tggtatgaaa gggaggggtt aaagctagag 9780
atggtggtag aaaaaaatgc agggttccta aaggactgag attcctggat ggaatttcag 9840
ggaaggggaa aatttctgga tatagtgact ggggagttaa gggtgtctag tccaatggct 9900
tttattttct tggaagggta ggcaaggcca acagccacat gtgtgggagg agatggttag 9960
aggggagagg aggtttgaag gcaccgctat ggagaattgg agagagctaa ggaaagacag 10020
aaagactgca gaaagtgctt agggttccac tgaagcggaa atagtgattt gtagtgatac 10080
aacccttatg agttatttga tttttttttt tttttaagca gcatctggca gtccaagtat 10140
agggctgaca gtttgggatt tttctttcca tgttggtgta aaagaagaac agtgtagtga 10200
aggaagttag gacaaaagaa tgattgaact gacaccaagt tttcttgatt tggtagaaaa 10260
ggaaataaag atagagcaga gatattgaaa agaattagag aggggttcaa gagactgaag 10320
gcctgggtga ggtcagagag caggtgtggt agacataaca gagagaacta caaggataga 10380
aagtgtggtt ggagagtggg aaggcaagat ttattcagta tgggggcttt tctgggtgat 10440
gacagcatct ggagtacagc cattgtcgtg agtggcccaa gtgtagcaga gataaagcgt 10500
tgttggagtg aaggaagtca aggaactgag aggctggcct agatggggat tttggttgtc 10560
atccatgagg atattgaagt catccaggag aatagcaggc ctgggggaca ggaaggaaac 10620
tgagccactt acagtgtctt cagtgatagg aaagcacagg gcaaaaagct ttcaagaaca 10680
gggactgtta agccgggtac agtggctcac acctataatc ctagcatttt gggaggccaa 10740
ggcgggtgga tcacttgagg tcaggagttc aagaccagcc tggccaacat ggtgaaaccc 10800
catctctact aaaaatacaa aaattagcca ggcatggtgg cacgcgcctg taatcccagc 10860
tacttgggag gctgaggcag gagaattgct tgaacctagg aggcggaggt ggcagtgagc 10920
ctagatcgcg cccttggctg cgatccagac ttcactccag cctgggtgac agagcaagac 10980
tctgtctcaa aaaaaaaaaa gaaaatcaga ctcttaatat ttgtaaagaa gtagtccttg 11040
agctactact taagtctaga aagagttgat attcttgttt taagagtgtt agggcacttt 11100
gggaggctga ggcaggtgga tcacttgagc ccaggagttc cagaccagcc tgagcaatat 11160
ggggaaacct tgtctctact aaaaatacaa aaattaacca ggcatgtggt acgtacctgt 11220
agtcccagcc acttgggacg ctgaggtggg aggatcacct gagcccagga aatggaggtt 11280
gcagtgagcc aagattgcgt gactgtactc tagcctgggc aacagagcaa gactctgtct 11340
caaaaaaaaa aagggcgggg attatcatag tgccattatt attatgagtt tatgatggct 11400
ttctctaagc accttttaca ttcggcattt attcagtacc tattaagcat caaggagtcc 11460
agaaaaaatt ttatatataa atatatataa aatatgtaaa tatatatatg catatgcttc 11520
cctatctcag gaaggaaata tgtgaacatc aggaaccgaa gtctactcag ttacatgcca 11580
ttggatatat cacacaaagt gctgagggaa ctcagaaggc tcattatatc tggggagtgg 11640
gaaggaggca cagagatgtg ctttgggaag tttaaattaa aatagcaaat ggggaaaatg 11700
aagacacacc agacagggca caagcaaaga gacatgaaag agtaagtcat gtgtttgagg 11760
atctggggat ccactagttc tagagcggcc gccaccgcgt agcagttacg g 11811

8

1241

DNA

BAC-F2 contig 4

8
tcgtgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagatccgt 60
cgagttcaag agaaaaaaaa agaaaaagca aaaagaaaaa aggaaagcgc gcctcgttca 120
gaatgacacg tatagaatga tgcattacct tgtcatcttc agtatcatac tgttcgtata 180
catacttact gacattcata ggtatacata tatacacatg tatatatatc gtatgctgca 240
gctttaaata atcggtgtca ctacataaga acacctttgg tggagggaac atcgttggta 300
ccattgggcg aggtggcttc tcttatggca accgcaagag ccttgaacgc actctcacta 360
cggtgatgat cattcttgcc tcgcagacaa tcaacgtgga gggtaattct gctagcctct 420
gcaaagcttt caagaaaatg cgggatcatc tcgcaagaga gatctcctac tttctccctt 480
tgcaaaccaa gttcgacaac tgcgtacggc ctgttcgaaa gatctaccac cgctctggaa 540
agtgcctcat ccaaaggcgc aaatcctgat ccaaaccttt ttactccacg cacggcccct 600
agggcctctt taaaagcttg accgagagca atcccgcagt cttcagtggt gtgatggtcg 660
tctatgtgta agtcaccaat gcactcaacg attagcgacc agccggaatg cttggccaga 720
gcatgtatca tatggtccag aaaccctata cctgtgtgga cgttaatcac ttgcgattgt 780
gtggcctgtt ctgctactgc ttctgcctct ttttctggga agatcgagtg ctctatcgct 840
aggggaccac cctttaaaga gatcgcaatc tgaatcttgg tttcatttgt aatacgcttt 900
actagggctt tctgctctgt catctttgcc ttcgtttatc ttgcctgctc attttttagt 960
atattcttcg aagaaatcac attactttat ataatgtata attcattatg tgataatgcc 1020
aatcgctaag aaaaaaaaag agtcatccgc taggtggaaa aaaaaaaatg aaaatcatta 1080
ccgaggcata aaaaaatata gagtgtacta gaggaggcca agagtaatag aaaaagaaaa 1140
ttgcgggaaa ggactgtgtt atgacttccc tgactaatgc cgtgttcaaa cgatacctgg 1200
cagtgactcc tagcgctcac caagctctta aaacgggaat t 1241

9

1701

DNA

BAC-F2 contig 5

9
ataaaaaaca gttaattagg agtatctagg ttatgtgaag cattcatcac cyycctaytg 60
rcagaaawtw tcgwtaggca aattttatat twtaagtaac tttaacatga acacttctta 120
aactttggct cataatttca caaaaattag gctgcaagtc accatattca tcagatactg 180
gcagacacta acttctgcgg ctatgacacc aagcaatact gaaatctctt atctttccag 240
gggggttgtt catgtattca gtgtttgcaa agagttcctg ctgagctaaa cacagtccac 300
tgtgcactct acgaaagagt ccatgagaca agcatggggg agggtaggaa gtttaatact 360
ttcacaatgc ctgtggagac gctggcagtg atgaaagcct agaaaactca tgaaaggacc 420
ttttatgagc agggtgaatg tagagcacaa aagcaaagtc agatgaccca cttaaagctt 480
tgcctttact gatgagaatt cattctcatt ccagattagt ctctctctag aaaaagcaaa 540
ccttatataa gagttggaaa attaagatac aggaagtata attctactaa attccagttt 600
ttccttctca aatatcagcc taagtcctaa ggtctgtggc caaagacaga aaatacaagg 660
cgctgagaaa tatgctattt atcttggtgt aacaatctct gactgttggg gtttgaggaa 720
atttaagctc tacaatccat agatcagacc agaagtttag ggtagtaata ttatgagagg 780
aaatagtttc tttctggaac ttatataaag caaataactg gtaaacctga tttgcaaggt 840
aatgacagtc caagttcctt caaagcagag aaccacttat ttgctcattc attcaactaa 900
gttccttgtc ttgtgccagg ctggagagag aaagcagctc ctgtcctcaa ggagctcaca 960
tctcaggcat cttctcaccc tcctttctca tgttaaccaa aacatttcag gttcatcaat 1020
gaaactcttc atccaggagg cagataaaat ggcttctctt cattttgatt catttactct 1080
ttcttttatt tattttatta ttattatttt ttttttttct gagaaggagt ctcgctctgt 1140
tgcccaggct ggagtgcagt ggcgtgatct cggctcactg caacctctgc ctcccgggtt 1200
caagcgattc tcctgcctca gcctcccaag tagctgggat tacaggcatg cgccaccacg 1260
cccggctaat ttttgtaatt ttagtagaga tggggtttca ccatgttggt caggctggtg 1320
tcaaactcct gaccttgtga tccgcctgcc tcagcctccc aaagtgctgg gattacaggt 1380
gtgagccacc atgcccggcc tactctttct tttaaacaga gaaataagat ggaatatttt 1440
tatcccatct tttcttctgt aattaaaaaa ggaatacgaa gaaacttgac atagtctctc 1500
tcctcatgtg ctctcttact tcccatccca attccatgtt tgctctcttt ttcctctctc 1560
cttctgtttt gttgtgaatg aagaattagg taactagtcc aaaactacag agctacacct 1620
ggagcctaga ttcactggta gcaaatcact aattttctga aggtaaatgg gagaaaatgg 1680
gggtgggggg aaactcatta a 1701

10

1293

DNA

BAC-F2 contig 6

10
ggagataata agtatacact atgtgtgaag ggggtgtctc tattgttgtt gtggcgatta 60
ggtgagtaat tttacacctg gttgtgaata aagtccgaga ttgggggact cacgctttgt 120
agagtctccc aggacaatgg gttttgcccc cgtgcccaat taatagttaa aggttggggg 180
cttttcgatt cccttattcc aactggatag ggctcttgaa atgcccccaa aaaaggttga 240
ccctttcccc acacgtcaaa gagggaattc tcccgctaga ctacccttga acctgaagtg 300
cagtccctac agggtattct agcttgttag catcccccac tgtgaatcaa tcccttaaaa 360
taaacctata taagatgtat gtaatagagg actaatcttt aatataataa gcatatattt 420
aatataattt cggtactacc cccttatctg gggggggggt ggggggatat gttccaagac 480
tcccagtaga tgcctgaaac cacagatggt actgaaccct acgtaaactg tatttcattc 540
ctatacatgc aggctatgtg ttgtaatctg tagggtaacc actaaaagaa cagggtctat 600
aacttggcaa gagggaaaaa agctaggata gtaaaaaagt ctatcaatcc aaaaagcaag 660
aaaaaagaga aaaaggaaca tgctggcata ttattataag tattgtattt tattattagt 720
tattgttaat tttttactgt gcctaattta taaattaaac tttatcacag ctatgtatgt 780
ataggaaaat atatatctgt ggttttaggc atccactggg ggtcttggaa tataatgctt 840
cccccagata agaaggtact actgtaatta tattatatgt catattaagt atacattaat 900
tctactaggt agtagccaca ttatatatta attatattaa atatatatca tatagaatta 960
ttttaaggaa ttgactcata atagaagagg ctggcaggct ggagattcag ggaggagttg 1020
catttcaagt gcaaaggcag actgccagag aattccctct tgcttggggg aggtcagcct 1080
tttgttctat tcaaatcttt gaggaaaata gaaagcaaag aatatattaa ctatattaaa 1140
caaactaaat gttccaatta aaatacaaaa attataaagc ctaataataa aagccctcaa 1200
ttatatgctg tttaaaagag acatttttaa gcttaaggat atagaaaagt tgaacataca 1260
agcatggaat aaaataagca tgcaaaatac tag 1293

11

529

DNA

BAC-F2 contig 7

11
gctgaggtgc atcgcggtgg cggacgctct agaactagtg gatccccaaa caaaacctgt 60
ccctgctaat gatggtagac ccaatcagat ccccggagaa gccgaaatac ggaaaccata 120
tcagcatacg catggcatac atagaacccc atacatggat tgcttactca gccagatata 180
gaaatctatc ttcacgatag agatatatat atatagacac actgcatata cagatgtgag 240
atggaggctc actctgccac ccgtgctgga tctacagtgg cacaagctca gtccacagtc 300
acgtcgatct gccgggcgtg accgactgag atgcagcggc ctcgggcgta gctgtgagta 360
cacgcaccag tcatcgcgac tggctgcaag tggtataagc ggaggggaca gggttacagc 420
atgacggcta ggcaggccgc aaactgagga ccacaagagt gccacgctgc ccgaacgcat 480
gcagtggcga gattacatgg ggcagccact agagccgccg tatcagaaa 529

12

18073

DNA

BAC-F2 contig 8

12
agcgcgtgcg ccgctctaga actagtggat cccccgagga gtgaggagga cctcaaccct 60
acttcctgaa atggagctct gagatgttgg agtagaaatt tggaaaccag agagagaagt 120
aagggtagtg ttgttgcaac atgcattgta tatggggggt cgggaagtca caggagtttg 180
cctcaaagtc tttctcggag acggatgagg ttttcactgt gattttcctg gtcgtggtct 240
atggatatag tacctgttag tgacatggat cttcttaact tctgatgtgt cttttcctcc 300
ctagtgtacg cataccaatt ctctccacag cttccatcac catgcatttg ttcttttccc 360
ttgttcttgt attacctttc tggaaaggaa tttttattgt aggctaattg ttactcccac 420
cagtatttaa ccactggata tttcatatga ttgatctctt ctgatttgga aaataaaaat 480
gtaatctcat tatattcatt tgattagtgg ggacagtcaa cacttctttg tgtattttct 540
tagctgttcg tttttctcgt ctgtaaatta tctgtttagg tccttcagat ttttcaaaat 600
tggactgtta tgttttcagt attgttatga gttcttgttt caattattta tgacagttca 660
ttttcttttt taaaatagac tttttttttc ttagagaaat aagaaaaaat aaaaattaaa 720
atagactttg tgttttagag agtttcaggt tcacagcaaa attgatcaaa aagtatggag 780
agttccggcc aggcgcggtg gctcacacct gtaatcccag cactttggaa ggccaaggtg 840
ggcagatcac aaggtcagga gtttaagacc agcctggcca atatgatgaa accccatgtc 900
tactaacaat acacaaatta gctgggtgtg gtggtgcaca cctgtaactg tacctactca 960
ggaggctgag gcagaagaat ctcttgaacc tgggaggtgg aggttacagt gagccacagt 1020
catgcccctg cactccagcc tgggcaacag agtgagactc cgtcctaaaa aaagaaagaa 1080
agaaaatata gagcattcct aaataccacc tgtccccaac acctgcacag cctcctcatt 1140
atccacatcc tacaccactg tggtaccttt gttgcaattg atggaccaac attgactcct 1200
cattatcacc caagctttgg tgttgtacat tctgtagatt tggacaaatg tataatgaca 1260
tgtgtctacc attgtagtat catacagaag aatttgactg ccctgacagt cctctgctcc 1320
acctgcttac tcctctctcc cttttcctaa ctgcacaacc actgattttt tttttttttt 1380
tttgagaggg ggtctcactc tgtcccccag gccggagtgc agtggggcca tttggggtca 1440
ctgaaagctc cacctccggg gttaatgcaa ttctccggcc tcagcctccc gggtaactgg 1500
gattaaaggg gcccgccacc aaatcggggt aatttttgga atttgaagta aaaagggggt 1560
ttccccattt tagccaggat ggtctcgatc tcctgacctc gtgatccgcc cacctcggcc 1620
tcccaaagct gggattacag gcatgagcca ccacgcccta cctttttttt aaaaaacaag 1680
gtcttgctct gtcacccagg cctgagtgca gtgatgatca ctcctcactg aagcgtcgac 1740
ctcccaggct caagtgatcc tcccacctca gcctcctaaa tagctgagac tacacacaca 1800
caccaccatg cccagctaag ttttgtattt tttatagaaa tgtggtcttg ctgtgttgtc 1860
caggctggtc ttgaactcct gagttcaagc aatttgcctg ccttggcctc tcaaggtgtt 1920
gggattacag gcatgagtca ccgcacctgg ccttttttat tttctttttt tttttttaac 1980
cagtgatctt ttactgtctc catggttttt cacattggct tctgtcactt agtaatatat 2040
gtttaagttt cttctacgta ttttcatgtt tttagcttat ttctttttag cagtgagtaa 2100
tatttcattg tctggatgtg ccatcactta tttatccatt cgcctgctga aggatatctt 2160
gattgctccc agtcgtggca attataaata aagttgctgt aaacatccat gtgcaggttt 2220
tttttaagtg gcataagttt tcatctcatt tggttaaata ccaaggagca caattgctgg 2280
atcatatggt aagagcttat ttattttttt gagagactac caagctgcct tccaaagtgg 2340
atgtaccatt ttgcattccc accagcagtg aatgagagtt cctgctgctc catattctta 2400
caaacatgta gtattgtcaa atgttttgga ttttaaaacc aaaatccatt ttcatagatg 2460
tgtagtggta tcccgtttta atttgcaatt acctaatgac ttgatgttct gtgtcttttc 2520
agatgcttat ttgccgtact gtttatcttc tttggtgagg tgtctattca ggtcttttgc 2580
ccatttttaa tctggttgtt atttttcttg ttgagtttaa gaattctctg tcctttgtca 2640
gatctatctt ttgcaaatat tttctcctag tctgtggctt atcctctgat tctcttggca 2700
ttgtctttca cagagtagac attttatatt ttaatgaagt ccagactatc aattatgttc 2760
tcatggatca tgcctttgat gttatatcta aaaagttctc gccataccca aagtcatcta 2820
gattttctcc tgttatcttc ttggcatttt atagtcttat gattgatatt taggtctatg 2880
attcattttt agttaaattt ttgtgaaaga taataaggtc tgatatggat taatttttct 2940
atatgtagct gtcccgttcc agtatcattt gttgaaaaga ctatcttgct ccattttatt 3000
gcctttgctc ctttgtcagt tgactatatt tatgtgggtc tgtttatgat ctctgttccg 3060
ttccattgat ctgtttgcct tttcttttgc taataccaca gtcttaatta ccatagcttt 3120
aaagtaagtc ttgaagtcca atagcattaa tctttgactc ttctttaata ttgagttgcc 3180
ccttcagaat cttaatgtct ctccatgtaa actttagaat cagcattttt atattcacaa 3240
aataacttgc tgagattatg attgagattg cattgaatct ataggcttat ttgggaataa 3300
ctgacatctt gacaatattg agtcttcctg tccataaaca ttatttatga tgggcttctt 3360
ctttatgttt aggagctttt gttttttctg tcagatattc cacttctacc tttatgattt 3420
cttaattgcc ttttatgctt agaaagtttt tcctcatcct gagctcacat attcatttat 3480
tttcttttaa aatgtgtttt caagcattta atttttaaac ctatgtggaa tttattttgg 3540
tatatggaat gaggtggtgg tctaactccc tcctctcaaa tatgtagtta tttttcccaa 3600
aaccattttc tattaattta tcaagaatag acatgtatac atatacatat ataatagtca 3660
gccttccact tgttgtttga cccttgtgaa ggaaattgta tgagtttcca attttggatt 3720
aggctcaggt agtaattgag ctgggttctg ccagagatcc atgttaattc actatccaaa 3780
cagagttata aaatgtaagt tttatgaaaa tctaacagta tatcactggt ttaatgatca 3840
cagcctagga agaatgggga aattgtcaaa atcttctgtg gatgcacctg aaggccactg 3900
ctgaacccat ttccctgcta ggcacggctg ctggtaccag gggcaaactc ctggagtata 3960
tatgaaccac ctacatctcc ttctcttccc cccctaccct tgagattttc atgtgtccct 4020
taaggatgtg tgtcctactt cccttggaga gtcactacca cattgaacac tttagactgt 4080
gagtcctgtg aagatggggc tcatgagtgt attgctcccc agttgtttct ctagcactag 4140
ctcagtatag ggcataaaaa tctgaatgga tgaacaaacc actattactg gtggggacat 4200
gctactatct tacatggttc gaggtggaat aaaggttgag aacagctata taatgtgttc 4260
cttgaagggc agcagtacat cagtgcaatc agcctacctt ctccatactt ctcactctga 4320
aaactgtaaa gctgcaccta gcaatcaact tgggagcttt aaaagggact gctccctagc 4380
tctcacccac aaagctgtag tctagcacag gtgacttttt taaaaaagtt ttttggtcca 4440
gatgtgatga ctcacgcctg taatcccagc acttcgggag gctgaggctg ggaggtcacc 4500
tggggtcagg agtttgagac cagcgtgacc aacatggaga aaccccatct ctactaaaaa 4560
tttgccgggc atggtggcac atgcctctaa tctcagctac tcgggaggct gaggcaggag 4620
aattgcttga acccgggagg cggaggttgc cgtgagccaa gatcacacca ttgcactcca 4680
gcccgggcga cagtgcaaga ctccgtctca aaaaaaaata aaaaaggagt cctattaaga 4740
cttattttta caggttggat atctctaatc ccaaaatctg aaatgctcca aaatttgaaa 4800
ctttttgagc gcagacatga tgctcaaaaa aatgctcact gggacatttt ggatttcaaa 4860
atttggatta gggactaggt gtgggagctc acacctgtaa tcatagcact ttgggaagtt 4920
gaagcaagag gatcagttga acccaagagt ttgagagcag cctagacaac atagtgagac 4980
gccgtctcta cagaaaattt taaaaattag ccaggcatcg tagtacatgc ctatagtccc 5040
agctactcag gaggctgaga cagaaggatc acttgagtcc aggaggtaga ggctgcactg 5100
agctatgatc ataaccactg tctccatcct gggcaacaga gcaagaccct atctcttaaa 5160
aaaaatctga aacactgcta gtcctcaaga taagggatag tcagtcttta taaagactca 5220
attagttatt ggatatctga ggaagcatgc atatcaggct cccaaaagat cattggttta 5280
ggcacacatt ttaatagctt ggaaatccag aatactcttc tggtgaccag ctcagacata 5340
gtcctgataa tataggacct catctaacat gactccctat tttccagata agcatggatt 5400
cctggttcat tcttgttctg ctcggcagtg gtctgatatg tgtcagtgcc aacaatgcta 5460
ccacaggtaa attgtcattt gataaggctg ctatttgaaa tgaaattttg ctttcacatt 5520
taatgagcca catttgaaaa ccgagatggt atttgaagaa aggaatataa aaattttatt 5580
caaagtgatg gtaaaatagg tgtcttcaga aatcttggaa ttgaatgctc agcattgttt 5640
ttcatacata cataactgct ttaaataaat caaagagatt atgtgttctt tcctgaaaag 5700
taaaataaat tgttgacatt tacaactcta tatatggttt ctgaggaact aagtgaagaa 5760
tcttgtgtct ttctccctta aaccgtagtc ctttggagga ggtaggaaag gtccagcatg 5820
agataaaaac gtagggggtg ggtggtgttg agggggattg gtctttgctt ggtctccata 5880
tgtttgagag tttattaagg cttgctgctt tgtgtctcac agctttttag cctcacattc 5940
ttcatgtgct atttccttgt tttttggtgt ttgtagttgc accttctgta ggaattacaa 6000
gattaattaa ctcatcaacg gcagaaccag ttaaagaaga ggccaaaact tcaaatccaa 6060
cttcttcact aacttctctt tctgtggcac caacattcag cccaaatata actctgggac 6120
ccacctattt aaccactgtc aattcttcag actctgacaa tgggaccaca agaacagcaa 6180
gcaccaattc tataggcatt acaatttcac caaatggaac gtggcttcca gataaccagt 6240
tcacggatgc cagaacagaa ccctgggagg ggaattccag caccgcagca accactccag 6300
aaactttccc tccttcaggt actagagatg attctgtttg ttcttttgct ctttgagttt 6360
agtcttcctt ttattatctt gtttgtgttt tctagcctta aaatttcttc aaataagtaa 6420
aattgctcaa gtgaagtaat gaaacctgta tgtggaattt ttgggttagc atgagtgaag 6480
aggaaagaag aaagattctg gagaatatct ttctgctagg tgggatcctg gttagattga 6540
gaggacttaa atgtgtttaa aggtagagaa gaaggcttaa aaagacaaga gaaatagagg 6600
agctcattga cgatgcaaga gactgaagat gaaaagatac agagaatgag taataagatt 6660
aggtttggaa agggagggat ccgtggagac catggaaagg agaatgggta ttgatgtcca 6720
tgacagttag atgtgagata cagagaatga gtaataagat taggtttgga aagggaggga 6780
tccatggaga ccatggaaag gagaatggac attgatgtcc atgacagtta gatatggagt 6840
ggcaggccag tggccagggg tggcatcagg ctctgggaaa tggttacatt gcagtgccag 6900
ttgttcaggg cctcaggttg aagcagtagt cccaaggaga aaatcagaga cgtggatctg 6960
agaccagggc aggtaagaca agtttctgac ctctttgaac cttaggtacc ttgtctgtaa 7020
aagaggatta gagataccct caaagggctt ctatgaggag taaaggaaat aatcattacc 7080
tgattgctat gtaactgtca tcccttttct agcaaaaatc actctttcct cttctgtgtt 7140
cccagttaga tggtgagtgc ccctaagcag aatcacatct cgctcatgtg gaacattcag 7200
gaactgtttg ctcagttgat tctcatttgt tactacagat gatatctttt actgcgcctt 7260
ataactcaga cccttcacct gccagctttt ccccatattt tctaccgtaa agacaagaca 7320
gcatttgcag ttaagagcac agtcttcagt gccacactga gtttgaatcc cagctcttcc 7380
ataaaccagc catgtttatg gcatagctgg cttactttat ctctctacct cggtttgttc 7440
atctgtgaaa caagaatgag tgatagtaat agttcttacc tcatagagga gatattagga 7500
ttaaacaagt taatatgggt aaagcactta taaaggtgcc tacacatggt aagcactatt 7560
tttaagtgtg agctgttagt attgttgtgg ttattgctct gatagttacc agtaaaatat 7620
atgaaggtac ctttaatgca gatggcatcc cactattctt gatgagatag gggactgcag 7680
acaaataatg tctgatactt gctttgtgct ttagagttaa tgtagttttg tcatagttat 7740
tactgtgtgc taggcatcgt actaagagtt ttctagaata atcctatgaa ttaagttcta 7800
ttttatgttt tataggtgaa agtattttac aatgatgaaa ccataatttg tggaatgttt 7860
ttcagtgtac aggtcatgac acaattcatg aaatcacttt agcaggccac cactagttgt 7920
ttgttttgtt ttattttaat ggatgatcca gttccatgtt tattctttta atgttacata 7980
caattttttg aaattttagt aacaacataa aatgttgggt tgtggccatt gcttagggag 8040
aaaggcagga taacttgtac aaactgtatg agtgaatgga aaaggtggag actgtaacac 8100
aggcctgact gactgaacag cccatgttct attgtgtact gtctttcatt taacagttct 8160
gtgacatgac catggataat catctccttt taacagatgc ttgatttcag actgtatata 8220
gaggttaaat gatttgtttt agatctcaag gctgacaaat taggcctatt tctcactttt 8280
gcggtctttc cactctgctt gtagggaact tagttttcca taaactgact taggtccaaa 8340
ttgtgccaca gctaagaatc tagttattgt acatttaaca cagttcacgt cataggaggc 8400
tgagactatg tttctctagt ggcgtttatt caagatgagt aaaacacaag aaaccattat 8460
cgcacatggg aatttcatag tcttaaaccc cacatcccac ttatcaccac catttaccag 8520
tcctcctgta acagttacaa ttttttatta aatcagtatt tgatgtatat tattgtaatt 8580
atgaaatatt cattgctgag ctataagtat aaatggattg tttttcttgt acagtttttt 8640
ttctggattt aatacttacc ttattttttg tttatttagt tttctattta gtcaggccag 8700
gcacactggc taacacctgt aatcccagca ctttgggagg ccaaggtgga cagatcactt 8760
gagctcaaga gtttgagacc agcctgggga acatggtgaa accccatctc tacaaaaaat 8820
acaaaaatta gctgggcatg ggtgcatgtg cttgtagtcc cagctactca ggagcctgag 8880
gtgggaggat tgcttaagcc caggaggttg aggctgcagt gagctgtgtt cataccactg 8940
cactccagcc tgggtgacaa agcgagacca tgtctcaaaa aagttattgc tactcaattc 9000
ttaccatgct ctccagagcc tctcaaaaca gctttctaca aagtgagatc tgttagataa 9060
tctatttctt ttttacctct agaaattcct cctgagccct ccattgtctt attccagtct 9120
aggcttgtcg atctctaggg ctactacaca gatacatcag cctgagattt cccttctctg 9180
tcattctggg aattcccctt gctgctgctt cctgacttcc atattgtctt cctttttgtc 9240
ttctcatcat tcggtagatt cctgagaaaa ggggtccatg ggaggcaaat tgcatcctta 9300
catatctaaa aatatcttta gggctgtgca tagaatttga ggaatatttt tcccccagaa 9360
tttttaaagt aatgccctaa ctgacacctg tttaccaggt ttggaggatt ttactgctat 9420
cttaatccct aattgtttgt atgctttcta ggatcttctc tttatcatca gtatcctgaa 9480
atttcacaga gatgtatctt gatgtgggtc tttttcgttc attattatgg atacttaata 9540
ggccctttag agccttgatc ttgcatttct gaaaattttc tcccatttct ttgaaacctt 9600
ctccccctct tccttttttt tttttctcaa attcttaata tttggatatt ggatgtatcc 9660
tgaattaatt ctttaatctt taaaattttt cctttctgtt gatctttgct ttgagtcttt 9720
ttctcctttt aaaaataaac aaaggccagc taggcacagt ggcttatatc tgtaattcca 9780
gcactttggg aggctgaagc aggaggatcg cttaagcccg ggagtttgag accagcctaa 9840
gcatcgcagc aaaacctcat ctctacaaat gatttagaaa ttagcagggc ctaatggctc 9900
atgcctgtgg tcccagctac tcagggctga ggcaggagga ttacttgagg cctggcagtt 9960
gaggctgctg cagtgagctg tgatcgcacc accgtactcc agtctgggca acagagggag 10020
acctcatctc aaaaataaat aggcctggtg tggtggctca ctcctgtaat cccagcactt 10080
tgggaggcca aggcaggtgg atcacttgaa gccaggagct caagaccagc ctagccgaca 10140
tggcaaaacc ctctgtctac ctactaaaaa taaaaaaatt agtcaaacgt gttggcatat 10200
acttgtaatc ccagctactt gggaggctga gacatgagaa ttgcttgaac ctgggaggtg 10260
gaggttgcag tgagtcaagt ccctgcacta tagcctgggg aacagagtga gacccgagac 10320
tctatctcaa aaaaaaaaaa tcagtgacaa gtaaaaaggt agaatacctt tttttttttc 10380
tttgagacag tctcaccctg tcgcccagtc tggagtgcaa tggcgcagtc tcggcatact 10440
gcaaactctg ccttcagggt tcaaacaatt ctcctgcctc agcctcctga gtagctggga 10500
ttacacatgc ccacgaccac acccagcttt tttttgtatt tttagtagag acaggtttca 10560
ccatgttggc catgctggtc tcgaactcct gacctcatga tccacctgcc ccggcctccc 10620
aaagtgctgg tattacaggc gtgagccact gcgcccagcc tagaatacct tttaaaaata 10680
aataaatagg ccgggcgcgg cggctcatgc ctgtaatccc agcactttgg gaggctgagg 10740
cgggcagatc acgaggtcag gagatcaaga ccctcctggc taacatggtg aaccccatct 10800
ctactaaaaa atacaaaaaa aaattagctg ggcgtggtgg caggtgcctg tagtcccagc 10860
tactctggag gctgaggcag gagaatggcg tgaacccagg aggtggagct tgcagtgagc 10920
cgagattgcg ccactacact ccagcctggg caacagagca agactctctc tctaaataaa 10980
taataaataa ataaataaat aaataaataa ctccttttac aaaagcatat atattcattt 11040
tttccattta taatataaat aatagatatg ctgagttgat ttctgcatat tgctttttca 11100
gttaccctat catacttgct ctttgtttta gtaaagagct gctgtattga aggatatacc 11160
ttaatctctt tatccagttt ccccatcagt ggacactaag attgttttca gagtactctt 11220
ataaacaata cagtttgtca tttcagacac atatgagaat attagcagga tgaattattt 11280
taagtctgca tttataaatt tatggatatt gccacattta cctctgctag gaagtctatt 11340
cctattaaca atatgtcaaa gtgcctattt ttctaaactc tcttcagtgt ggtgaattgt 11400
taaacttggg gatctctgcc aatctgacag gtgaaaaata acatctcagt gtaagtttaa 11460
tttgcatttt gctgagattg agcaattttg tgtaatttaa aagatcattt atttttctga 11520
gcattctctg ttgatattct ttacccattt ttattagagt gtcaaggttt tcctgactcg 11580
tttgtagatg ttctttgtac gtttgggaaa tgagtccttt gcctatggta aaactgcaaa 11640
tgttgttccc taggtggtca tctagatttt ctgcattgca gaagatatca ttagctattt 11700
ttaatttttt taatttaaat atttctcagt ttaggttttc taggaattgg gtcatatcta 11760
ggaaggcttt ccttactcca agattataaa aataattttc ttctggactt ctatggtttc 11820
gtgtgtgtgt gtgtgtgtgt gtacacgcac ttaagtctgt ctcgaattta ttctgatgca 11880
gagtgagcta tggatctgtt tttccccaaa tatctaactt gtcccaatac cccttaataa 11940
tttatttttc ctcattgatt tgaaatgcca cctatcttat atattgaatt cagatattta 12000
tttacctctt catatgtatt tgagtatttg ggaacattca ttttattttc tattaatctt 12060
tttctctgtc catgtgcaaa gcctcactgt ctcaataatt gtaactttgt aaagtattta 12120
atatccagta aaatgagtca ttccttgtta attttatttt tcagaatttt gttagcaatt 12180
cttattataa acattagaat taacttgtct agcaggaaaa aaagtttgta ttgatcatgt 12240
taaatacgta gattaacaga gaaaatggca tcttacagat gttgagtcta actatccaag 12300
aatgcaatat attccatttt ctgaagtctt ttttttttaa atcttctgtt tttgtaatta 12360
taaatggagc attttcttcc atcagatctt ctaactggct gctgttgggg atatgaaggc 12420
tactgatttt tgtagagaca ttttgtactg gccaccttaa actctcttag tattggaagt 12480
aattttcttc attaattttt atggcttcaa gtcatctcat ctgcatatat cttccaaatt 12540
tttagaactt tctttttctt ctgtttaatc gcattgatga atacctccag aacaaagtta 12600
agcagctggt aaatgcagac agcattctct tgtatctgac actaaggagg acactttcag 12660
tggtttttca ttatacgtgg tactgactct tgagttgaga taaacatatt ttattgtgtt 12720
caggatttaa tgagcgttta tgttaggaat gggtgttaaa ttttgccagt tgcctgttca 12780
ggatcaatga gaaagatctg aatgattttt tttctctttt ggtctgtttc tatggtggat 12840
tctattccta ggtttgtttg tttgtttgtt tattttgaga tggagtctgt taccaggctg 12900
gagtgcagtg gcgccatctc agctcactgc aacctccacc tcgcgggttc aagtgattcc 12960
cctgcctcag cctccgagta gctgggacta caggcacgca ccaccatgcc cggctaattt 13020
tttgtatttt agtagagacg tggtttcacc atgttggcca acctggtctc gaactcctga 13080
ccccatgatc ctgcctcagc ctcccaaagt gctgggatta taggtgtgag ccactgcgcc 13140
ctgccagttt ttatttattc attttttaga gacagggtct tgctctgaat taattcttta 13200
atcttcttaa tttttctttt ctgttgacct ttgctttgct ttaagtcttt tcctttgagt 13260
catccaggct gaagtacagt ggcacgatca tggctcactg taaccttgaa ctcccagact 13320
taagcaaacc ccacctcaga cttctgagta gctaaggact ataggcgcat gtcaccacgc 13380
ccagctaatt tttaaatttt ctcagaaaca gggactcact gtgttgccca gactggtcat 13440
gaactcctgg cctcaagcag tcctcagcct tagccttcca aagcactggg attataggca 13500
tgagccaagg ccgcccaaac atattgtatc gttcctgtaa caagctgttg cagtctattt 13560
gatattattt cttatttttt tcatttagaa ttttctctgt ctagatattc tcaaattatc 13620
tctaaatgag attgatctat gtttttcctt tgtgtgtgta ttctttttga taagttttag 13680
tttttagtgt tttgttttgc tacatggaaa ggatttgaaa gtttacacta aaaaatatgc 13740
tttttttttt taagacaggc tttttcactg ttgcctagtg ctggagtgca gtggcatgat 13800
ctcggctcat tgcggcctgc acctcctggg ctcaggtgat cctctcacct cagcctccca 13860
agtagctggg attacaggtg tgttccacca tgcccagcta attttttgta tttttttgta 13920
gagatggggt ttcgccatgt tgcccaggct ggtcttgaac tcctgggctc acatgattct 13980
cctgtcttag cctcccaaag tgctaggatt acaggtgtga gccaccacat ctggccattt 14040
cattcatgtt ttcaaatgta tttgaatgag gaaaagttct cccttgtgat tatttattat 14100
aatagcctac agagctatta atttttaaat tttgtttact ttatgtctcc tttttttttt 14160
tgtttaggct gaataaccat ttatttcata ggtttattgc cttttttctt ccaaagaact 14220
tgctattgtg catttatagt ccttttatgt ttacgttttc tatttcattg attttaactt 14280
tctaccttct ttagatttat tttgttcttt ttctatcttc ttgaattgag tgtgctttaa 14340
ttgcattctt tccagttaat taacatattt agtgctgtga attttgaaca agcacagctt 14400
tagccacatc ccataggtgt ttctataggc agttgtatta ggatgcgcta taagctgctc 14460
tgacaaagat accaaaattc agtgacttaa ataagaccaa agtgtctttc tctccccagt 14520
tacattccag aggtagacag ggccttcgtc tcagtaggga ccaaattcct ttcctcttgt 14580
ggccctgcca tcctaacaat attgccctta tctgtttggt tagagatagt tctcaccatt 14640
gggttctagt tccaaccact gcgaaggaca aacaaaggga ataggggcca tttctcttcc 14700
aaaagatgtg acctggaagt tactcacatt gctttagctc acatcccgtt ggctagaatt 14760
catcacatga ccacacctag cacaaaggag tctcaaatat agtctgccag gagagcttgg 14820
tgctcagcta aaaaacaaag gttctgtatc aaggcaagaa gagaaagaga ctgatctgag 14880
gggaggagag ttggcaggtt ctgtcacaaa acttctcgtc attgttattt ttaaggtatt 14940
tttccatttt gggttttttg tttgtctgat tttttttttt ttttttgaga tggagtctcg 15000
ctctgttgcc caggctggag tgcagtggcg tgatctctgc tcaccgcaag ctctgcctcc 15060
tggttcacgc cattctcctg cctcagcctc ccaagtagct gggactacag gcgtacacca 15120
ccacgcctgg ctaatttttt ttttgtattt ttattagaga cagggtttca ctgtgttacc 15180
caggatggtc tcattctcct gactttgtga tctgcccact tcggcctccc aaagtgttag 15240
gattacaggc gtgagccacc gcgcccggcc gtctgtttga tttttgagat ggaatctcac 15300
tctgcccccc ttctggagta cagtggtgtg atcttgggtc actgcaacct ctaccctccc 15360
aggtttaagc aattcttgtg cctcagcctc ccaaagtgct gggattaaag acgtgagcca 15420
ctgtgcccag cccattttgg ttttgatttt tttttttctt tgaaatagag tctcgctctg 15480
ttacctaggc tggagtacag tggcatgatc tcggctcact gcaacctccc cctcctgggt 15540
tcaagtgatt ctcgtgcctc agcctcccaa gtagctggga ttataggcac ccaccaccac 15600
gcccagctaa tttgttttgt atttttagta gagacggggt tttaccatgt tggccaggct 15660
ggtctcgaac tcctgacctc aggtgatcca ctgcacccgg cctcattttg gttttgattt 15720
ttattttcaa atgttttctt actttgtcaa tttctaattt tattgcattg ggacaaaaga 15780
atattgtact ctttctactg ttggggttta taagggctgt ggatatttca ctcgcctttg 15840
aaaagaaggt tttctctgtt agtctgtaga gtttggtatg taccaattag attttattac 15900
ttatcatttt ggtcttttgt atccttactt aattttgtcc tcttgaattt taatggagca 15960
aaagacataa agtcctctaa taacatgcgt tctgtttgca ttctcatact ttttatgaat 16020
attgatgctg cactatttgt gtacccaggg agaaggccag accactgtcc aaagtttagt 16080
gaatctgggc agccttgttt cccagttgtt ggaggatgcc tcatggagga aagcattcct 16140
aatcctggag cttgttttgt tgtactctaa ttgaattgta atgtgtttct ttaacctgaa 16200
tgaatgtttc tattttttac ttattacaca ggtaattctg actcgaagga cagaagaggt 16260
gagctgctca ccttatatct gttgttcctt ttacacagtg tacagtattc atttatttcc 16320
tctgctcaca gtctgtggta accgtgtgca tctgtggctg tgttgtttgt ttactttccc 16380
ttaagttatt tccatgttaa tctcatggag aagagcaata gaaacaagta ctgtattcag 16440
tatgtttttt aatatagact atggattcta acagctatga tgtattttaa caagtaacaa 16500
aatatatctt actttgacat gtcactttgt taacattact ttttggtgat attaggtcat 16560
aatttctata ccattagtta cttctgattt ctaggccaca gttcccttta aatattcttt 16620
gtgttgtttt tcccctagtg tataaaatgt caaccctttg tggctttata tggattttat 16680
ggattttcag cccttaaatg taaagtctct atggcctgag atgttgtgtc tgtggtttaa 16740
gctggactgc tgagtccctg gtcactagag agtaggggga catgggtact tgtctgcaga 16800
agtgtggcac attttgccta gaatgacagt aaggctgcta tcaaagagca tgagagaaag 16860
agaaagagat catctaacat tctaagaagt gattattaca tttgagtttt aaaaatgtta 16920
ctattcgaag cagtgttttt atcataattt tctattttat caaatcagac ttgagttttt 16980
tttctgattc tgttatttaa ccatacacaa ttttccctgt gtaattaagt aatggaacac 17040
ttggaggcat atgaagtccc actaagtagg gagcatttga gtcagaaaag tgggtactct 17100
cttcctttat gtgatgtcca tctgccattg tatttggtaa ggaatagtga ggtgttacca 17160
tactgtgtac agatttccct cacttttcca cctctcactt tcctaaactt gggaactaaa 17220
cattggatta atacagtgtc tttgctgttc agattcactt gccagatttt atcaaatgta 17280
gacttaaata ggttttattg tgatagatat ttacttgctc cctaaaactg ctctcttaac 17340
cagccttaca ataaagtcaa aagtcaaagt ggtaggcttc aagatgaaac ataagatctg 17400
ttgactcctt cctctattta gtatatattt tcataatatt cagccttttc ttgccccaga 17460
tatcatatct attttaccta cccaatattt aagtagtttc catgttgtga ttaagaaaac 17520
aaaattacca taattaccta gattattgct aattgtgaca tatgtaaagt ctattaatgt 17580
aataaatctc ctttcttaag tcaaaaaata attttgtgta attccaaaca ggaaactgaa 17640
aaggcatagg tattctcagc agtctctaaa gtcccaaaat ctaatggcaa ttttaccaga 17700
gcagatcttt agaagtattg ctataaattt ggatatccca ttctaatttt aagccaaatg 17760
ctttttgaga aataagccag ctgtttggaa atgcttgtat tataatcggt ttgataagca 17820
gttatgtctt atgcagatga attaggggct acctgttttt atgcactggt ctttggggtg 17880
cttttgaaca gtagtgtctg atgttttaat tgtcaaagca aaaagaaatg agagggaggg 17940
caacttttct tcctcttctg aattccagga aactggttat tttctcatgc catatgattt 18000
taaaatatat tcccagccag gtgcagtggg tcacgcttgt aatcccagat ttttgggatg 18060
ccaagcgggg gga 18073

13

7505

DNA

BAC-F2 contig 9

13
tccgagctcc acgcggtggc ggccgctcta gaactagtgg atccctctgg tggcccattg 60
agaatcaaaa cttgcagtga gtgactctat aaaatggaaa attgaatcaa gtctgaaaat 120
gatccacata gttctacagc agggctggac accgtggtca ggacctcaat atattctgct 180
tccacagaat tcagacagtt cagagtttgg tgaattaacc tcaaaggcag caagatatct 240
gtcccgggag tcagcaggta agcatagcag aaatggctgg agcagcggga gcctgctttc 300
cttctgttgg ctgctagcgt ccactccatt atagctcctg atggaagatt tctacagagt 360
gatgcctcag aatcttcctt atacctttct tccatgatcc ttgcacctct ttttctagat 420
ttgcccacat tcttatgtgc aagtaactag atatacatta tcagacaagc tagcagacct 480
gcatatatcc acttccctac ttttcctata atttcttcac ctgaacctct atcattcttc 540
tctttctgtg ttgactctgg tgttaacctt gcaggcaagt tgagcgtggg tttggtgtca 600
cagtgaagga ctaagggaat agttagcctt ctatttatta acaaatcttc cctttgatgt 660
ctggatcagt gtctctctaa taggaattat tggcatgtta aggcaaagaa catatgctta 720
ttgagtgctg actgattggg gttaatacta atttgatact attaaggtgt ggggcccagg 780
aatgccaaaa ttctacctca atgtagagcc accattcccc ttgaggtaac ctaggtggga 840
tagatatacg tgtaagggct aatggaagat agggaatcaa agtatcactt tattttttat 900
ttttattttt tatttaattt ttttgagatg gagtcttgct ctgttgctag gctgtagcgc 960
agtggcacaa tgaaagtatc actttattat tatctgagct tgtgccctaa acttcactgc 1020
agaatatgct ggtaaaatgg actggattac aggatttaga ggcaaggtcc acaggtcagg 1080
ataagaggta aagagggaaa tctttctctc ttcctaagcc caaaccctcc atgacaattg 1140
agattaaaaa aaaaaaataa actgatgaga gaatccaagc acagttgatc aaagaggaaa 1200
gagaaatgat gatgtttccc tctttctttt tcatgagaaa gtggctctct tattgatcgg 1260
ctacttgatt agagaaacag tgggggaaag aactgccata tccacatgtg caatttttta 1320
aaacacacag tgattctgaa cactagtata aattcccagt cagtgttctg gccatctgac 1380
tactcaggtt ataataccta atttttacaa gggagttggg aagtgtgcca aacctgtaga 1440
agtctatatc tactgtattc agattttata tgcattattt tatataacct tttgacctct 1500
ctcctctatc atcacttgag tgatttcatc cagcgtcatc atttaacata ttttaaataa 1560
ctctatatac tgataattcc caaatttata tctccatccc cgattgttct cctaacctcc 1620
agcctctaat atccaactgc ctactcaagc ctcagcaatg gtgagcgccc ctgccccagc 1680
ctcgctgctg ccttgcagct cgatctcaga ctgctgtgct ggcaatgagc gaggctccgt 1740
gggcgtggga ccttccgagc caggcgcagg atataatctc ctggtgtgct gtttgctaag 1800
accgttggaa aagcacagta ttagggtggg agtgacccaa ttttccaggt gtcgtctgtc 1860
acagctttgc ttggctacga aagggaattc gctgacccct tgcacttcct gggtgaggca 1920
atgcctcgcc ctgcttcggc tcatgctcag tgcgctgcac ccactgtcct gcacccagtg 1980
tccgacgagc cccagtggga tgaacccggt acctcagttg gaaatacaga aatcacccgt 2040
cttctgtgtc cctcatgctg ggagctgtag actggagctg ttcctatttg gccatcttgg 2100
aactgccttg cattcagttt ttaatatcca actgcctata cgatatcttc acttggattt 2160
tgaataggca tatcaaactt gtcatgttca aaagtgaggt tctaatcttc cctcccaaac 2220
ctgcttctcc catggctttc cccatctcag taaataggaa tttcatcctt ccaattgctc 2280
atgccaaaaa tttgggagtt atccttgact cttctctttc tcacacccca cattcaatcc 2340
atcaccacat tctgatgcct ctatcttcaa gatatactta gactttcacc acttttcttc 2400
actctgcaat taccactttg gtccaagcca ctgttatctc tttcttggat tattgtaata 2460
gcttcctaat aatttgtccc ctttcttcca cctttgtttc ccctacagta taatcttaac 2520
gaagcagcca gaatggttgc ctacaaacct ttaaaatggt aagccagaac atgtaggtat 2580
attcaaaacc ttccaatggc ttgtcatgga actaaaagtc tctacattgg cctataagac 2640
cctatgtcat ctacccctag tctcctcctt tctaacttca tctcctgcta tgctgtcctt 2700
caactcactc tgctccaggt gctctggcct cctcaaacac accacacaca cttgcagctc 2760
acagtcttgg cacttgctgt tcttctcctc taggaccttc ttcctccaac tgtctggttc 2820
acccacccct tccttctgga tttctgctct gatgtcattt tatcagtggg cacttcccaa 2880
tttctctatt taagaccaca attccaggcc agggtggtgg ttcatgcctg taatcccagc 2940
actttgggaa gccgaggtgg gcagatcatg aggtcaagaa ttcgagacca gcttggccaa 3000
catggtgaaa ccccatctct actaaaaata caaaaaaaat tagccaggtg tggtggcaca 3060
tgcctgtaat ctcagctact taggaggctg aggcaggaga atcgcttgaa cctggggggc 3120
agaggttgta gtgagccgag attgcgccac tgcacttcag cctgggcaat agagcgagac 3180
tctgtctcaa aaaaaaaaaa aaatttgctg ttatttccta tactattttt gtaaggcaag 3240
gaccttatta ttttccttga taatacctct cacactttat aattacatat ttgactttgt 3300
tgattaatga atatccctcc tttatagcat aaattccaca agagcaagga ttacatgtct 3360
gcttcattct cactgtacac ctaaaaccta gcacagggtc tcacacataa caggcacaaa 3420
acaaacaatg gattacgttg agccaaagaa caaaaaaaaa tagtaattta tcactaaatg 3480
tctttgttaa attccaacaa cagggggcag tatatcaggt attataagaa agtaattagg 3540
cacatcccag cactttggga ggccgaggcg ggtggatcac aaggtcagga gttcaagacc 3600
agcctggcca atatggtgaa accccgtctc tgctaaaaat acaaaattag cgggtgtggt 3660
ggcacacccc tctggtccca gctactcagg aggctgaggc aggagaatcg cttgtaccca 3720
ggaggcggag gtttcagtga gccaagatcg tgccactgca ctccagcctg ggtgacggag 3780
cgagactctg cctcaaaaaa aaaaaaaaaa agaagaagaa gaaagtaatt aggcaccttt 3840
ggcttaagac actgggctaa atccatgaat ttacttcatc ttcccccaaa gcacactgac 3900
atggtagaag aaatataaaa atactaatga atcaacagca tatctgaaag gcagcaaacg 3960
gtggcatatg tagatcagaa tctttgagag atttctggaa gacaaaacag accagactcg 4020
atgtccaaga gatcaaacag agccaaagag cctccagctg aaaactaagt actagttcta 4080
ccagtttggg cctggaaaca cctcaagctc agagggaatt gggactgggg ttgaaagtgg 4140
accttgaggt accaggatgg tacttaagca aaggcctgcc aacccagcac cagtacaccc 4200
acagcccaaa tgacaagcgg ggcttcccat ctagactcag ctggaaaaac agtgctctac 4260
acagagtaga gagtttgtca cagagactgg taagggcttc ttttttacaa aacatatgct 4320
gcatatatat tttctcaacg tcacactaat gacattttgg gctatacaat tctctgttat 4380
gtgggtctgt catgtgcact gtaggacatt taacaatatc cctagcctct aattattaga 4440
tgtctgtagc aaattcccaa ttttgatgac caaaagtatc tccaagcatt gctaaatgcc 4500
tttgtggggg aaatagcccc cagtaaggaa ccactggtct atactcacgc cattctaact 4560
gaattctttt aaggcaaatc cgagacctag catttcaaat gcaattactt aggtatgtat 4620
caccaagaga tcaagattct taacataaac ataatactat tatccaattt aaaaagtaac 4680
actaattcct tagtatcatc taatattatt cagttactgc ttgaatttcc ctgagtgtct 4740
cataaatgct ttttttttgt tttggttaga attgacacca gagcaggtct acactgcata 4800
tgattgttaa gtatattggg tccacagaag gtctcctggg gcctgcagac agaaaaaaac 4860
catagtagtg cccaagctaa ttctaggcaa ccacaagaga ggaaaggaaa aagaaaacgg 4920
cagctcgcct agaggataac tgcaccctgc cccgattttc ctgagccatc actgaacccc 4980
ttcctggttt aggacgtatg tccatgtttg tcttctgaag ggatgaaggg acacctattg 5040
tgagcacagt ctaagccact caatggtcca gggcatagct caaacagagc aacagtagcc 5100
ctgggaaatg gaggtgacaa aagaaacaga ataaatcttt caaaatatac tgcaatttgt 5160
gcaacaggat gccatattga tttaaaaaaa ttttttttct taaatttttt gtagagatgg 5220
ggggaggggg tcttgttgtt gcccaggctg gtcttgaact cttggtctca agtgatcttc 5280
ttgccttggc ctcccaaaat gctatgatta tgtgcgtgag ccactgctgc attgcgtttt 5340
tttttctttt ctcgagacgg agtctcactc cgtcacccag gctgaagtgc actggcgtga 5400
tcttggttca ctgcaacggc ctcctggttc gagcgatcct cacaccttag cctccctagt 5460
agctggaact gcaggcctgg ctaagttttg tatttttagt agagacaggg tttcactatg 5520
ttggccagcc tggtcttgaa ctcctgacct caggtgatca gcctgcctca gcctcccaaa 5580
gtgctgggat tataggtgtg agccactgtg cccagcctac attgatattt tttaaaagcc 5640
actatttaaa aaggagtaat ctgagtagta agaaggagtt ctttaaaaac tggccgggca 5700
tggtggctca cgcctgtaat cccaacactt tgggaggccg aggcaggcag atcacctgag 5760
gttggtagtt taagagcagc ctgaccaaca tagagaaacc ccatctctac taaaaataca 5820
aaattagcca ggtgtggtgg cacatgcctg taatcccagc tactctgggg gctgaggcag 5880
gagaatcgtt tgaacctgga aggcagaggt tgcggtgaac cgagatcgtg ccattgcaca 5940
ccagcttggg caacaagagc aaaactccgt ctcaaaacaa aacaaaacaa aaatgaaaac 6000
aaacaaaaaa acaccaacat gattaggagg gaaaaaatct agatagaaag gcttaacagg 6060
gccgggcacg gtggctcatg cctgtaagcc caacactttg ggaggccagg gtgggaggac 6120
tgcttgaggc caggagtttg agaccagcct gggcaactta gcgagactct ggtagtctgt 6180
ctctaccaaa caaacaaaca aacacctgat tagctgggca tggtggcata tgcctatagt 6240
cccagctacc cgggaggctg aggctggagg atcgcttgag tcccagaggt caaggctgca 6300
gtgagctgtg atcaggccac tgcactccag cctgggcgac agagcatgag tctgccccag 6360
ccctgcctcc aaaaaaagaa aggctaaata ggagaactga tataactgaa aaccaaatta 6420
gttgtgtgaa agagcaactg tcctggaagc tcccagaaca cagagcaata agagatgaaa 6480
aatatgacag catagaaaag aaaggaactg gataggtcca ggagatccaa tacctgtgca 6540
acaggagagt ccaaagaaga aaccagtaag aagggagaga agtaatacaa gaaagttcct 6600
gagttatcag gccaaaagaa ataatctagt ttgtggagta atattgacaa aaaaatcttt 6660
acacctagat gtattctgaa aaaattctta aattctaatt gaaatcaacc aacgaaccac 6720
aggccagcct tagaaaacca tttccagggc atggggtttt agggtctgac agacctgaag 6780
ttcaaattcc tactatccta acttactagt agtgtgataa tctcttagaa caatgtatga 6840
aatggaagca taatagcacc ctccaccttt tagagttaat gggagatcta aaagaggtaa 6900
catttgcaaa gtgtctgaca tgaagggaag agattggctt tggcatccac aagttcacac 6960
actagcagag aacctcagtc cagcttccta cgctcaggca gttctttgcc tagaagaggg 7020
gtcggcaaac tatagcccaa atttagccca ctgcctgttt ttgtaaataa aatgctatca 7080
gaacatggcc atgttcattc atttacatac catctatggc tgcttttaca ttacaaaggc 7140
agagctgagt agatgagaca gagacagtat ggttacaaac cgaaactgtt tcaaccccaa 7200
cttcattcca gcaaagtttt actttctaga ttcaggccag ggagcaagca tgaaaatgaa 7260
aaccactaaa atggtgtccc gggacaacag atacctactt gctataactt ctttccttga 7320
aaacaaaggg ccatattaat tgaagggctc acctctaaac aggtgagtga cttaaggact 7380
tcagacacac actggtcaac tacaaactag tcagtaaagg aatagccata gtcctatagc 7440
cccagttcct atggcccagg gggatccact agttctagag cggccgccac cgcggtggac 7500
tccag 7505

14

529

DNA

BAC-F2 contig 15

14
gctgaggtgc atcgcggtgg cggacgctct agaactagtg gatccccaaa caaaacctgt 60
ccctgctaat gatggtagac ccaatcagat ccccggagaa gccgaaatac ggaaaccata 120
tcagcatacg catggcatac atagaacccc atacatggat tgcttactca gccagatata 180
gaaatctatc ttcacgatag agatatatat atatagacac actgcatata cagatgtgag 240
atggaggctc actctgccac ccgtgctgga tctacagtgg cacaagctca gtccacagtc 300
acgtcgatct gccgggcgtg accgactgag atgcagcggc ctcgggcgta gctgtgagta 360
cacgcaccag tcatcgcgac tggctgcaag tggtataagc ggaggggaca gggttacagc 420
atgacggcta ggcaggccgc aaactgagga ccacaagagt gccacgctgc ccgaacgcat 480
gcagtggcga gattacatgg ggcagccact agagccgccg tatcagaaa 529

15

635

DNA

BAC-F2 contig 33

15
taccacgcgg tagcgccgct ctagaactag tggatcgggt aatccagcac tttgggaggc 60
caaggagggc agatcacctg aagtcaggag tttgagacca gcctggccaa catggtgaaa 120
ctccatctct actaaaatta caaaaattag ccgggcgtgg tggcgcatgc ctgtaatccc 180
agctactcga gaggctgcgg catgacagtc actcaagccc gggaggtaga ggttgcagtg 240
agctgagatt gtgccactgc actccagcct gggtggcaga gtgagaccct gtctaaaaaa 300
aaaaaaaaaa aaaggcccat taggggaccc aaacggttcc ccagctttgt tggatttccc 360
caaatttggg gccaattttt ggagggttgt cccttaaaaa tttaaatttg ggggtttttt 420
tccaggcgcc cattagaaat gggttccgaa aattttttgg ccaaaaaaat ttggtttaac 480
cgcggaccaa aatcctaagg tttaactttt tcctaaacct tttagaattt aaagtttccg 540
gggtttctca ggagggggta acccttcacc ccaatataac tcggaaaccc ccctttttta 600
ggaaaagggg aattagtggt gctttccggg ccaaa 635

16

938

DNA

BAC-F2 contig 39

16
cccagggacc aagcgagtgc gaccgctcta gaactagtgg atcccccttg aagactatat 60
ttcttttcat cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata 120
aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgg tttccgaaga 180
tgtataatag gttgaaagtt agaaattatt attataatag caaaaaaaat ttaaagttag 240
aaattagaat ttaaggctct acacacgttt acgatgatat tggacgaacg acacgattag 300
acagttgtag gttgtgtgtt gtgatgtttt tgagtgattt gtagtgttta accttgtggt 360
ttggaaaggt ngtatgagta ttaatctcgg gcttattggg aggtttatgt gcaatgcatt 420
ttgtggtttt tttataatgt tgtgtttagg gttaaaacct gttgtgtata ttgtgttggt 480
ttgttgcttg tttgtacatt ggtatgatgc ctnttttgct tatgggttng gtgtttggtt 540
ttggttgtgt tttttgtggt gtgttgtttg atagttttag cggttgtttt tgggttgttg 600
ttttatgttg tggtggtgtt ttgtgtgtag agttgtggtt tgtgtgtttt gttggttgtg 660
ttgtggtatt gtttatgttt gtcgtgtgta tggtttgttg ttagtcgttg ttgtaggctt 720
gtgtgttgtg tgttgtgtgt gcgtgtggtc tagtttgggt ggtattgttg atttagtgtg 780
atagtctgtt agagtttggg ttgttgtgtg tattgggttt gtctgtgtgt ggtttttttg 840
tgggtgtaga tgatgatttg tgtatgtggg tgaggtatat gttatttgtg gtatttcggt 900
tgtgatgtgt tggttattat gtgtttgtta tgtgtatt 938

17

1145

DNA

BAC-F2 contig 41

17
gtctccgagc tcaccgcggt ggcggccgct ctagaactag tggatccccc gctctcactc 60
cctgactctt gccttctgta acaactggag acaactcttt caaaaccagc tccaagcccc 120
agacttctct ctgggcttta gttcgtaagg caggtgccct actgagtgag cctagatcag 180
acagaaacat agctgttggc aaggatttag gtgaatttcc ttccattgtt tttctaatac 240
cttttttttt ttttggaaaa tataaccatg cacctacaca catatttgaa tatcctgcct 300
ttttatttaa aatgacatga taggtccggg agtggtggct catgcctgta atcccagcac 360
tttgggaggc cgaggtgggc agatcacctg aggtcaggag ttcgagacca gcctggccaa 420
catggtgaaa ctccatctct actaaaaatc aaaaattagc cgggcatggt ggcaggctcc 480
cagctactca ggaggctgag atgtgaaaat cgcttgaacc cgggaggtag aggttgcagt 540
gagctgagat cttgccattg cactccagcc tgggcaataa gagcgaaact ccatctcaaa 600
aaaaaaaaaa aaaacccagg gataaacttt ccaaaaggcc ccaaaaaggg gcatgattaa 660
gacaataaat tagtcgaaaa ttgtcaatat aaatgaataa taattttttt ggccattctg 720
ccaagtggca taaccctgtc attctgccca ttcggcaact ctttttcctc ccggggaatc 780
gctcccactt tttgcatggg ttttggatgg aactgttggt cacaggtttt tcacccccat 840
ttggccctcc cagaggtgta caaagtaccc cagcctggcc ctttttcacc caattttccc 900
aggtatattc ccccggtttt ggtcccaggt tttaaccccc ccctccaaag ggctttgggt 960
tttggaagga ttaagtcctc gaaataggcc cctcataata cctggggggg ggaccttttt 1020
caaagttgtg ggcacctctt gtgtcgcccc cacgggggac tgatgtattt acgccccntt 1080
ggggnntaat atggattgnt atgtattggg cgaggagaaa atatttttga tggggttttt 1140
ctctt 1145

18

852

DNA

BAC-F2 contig 42

18
tcaccgcggt ggcggccgct ctagaactag tggatccccc gttttgctct ctccttagaa 60
tgagctggga actagtcact cttgttttct cacctataat agcatctggg tccagtgttt 120
tttatgtggg acaaatttga acttgtggtc aacctcttta attgtaagaa tattcaggtc 180
ttttgttctt cctgggctag ttttttattc tttttctaga gattcgttca tttttcttag 240
ttttatttgc ctataattgt ggataatctg ttttttatct gctacttctg taattatttc 300
cacatttgat ttataatatt aacttgtggg ccaggcgtcg tggctcacac ctgtaatccc 360
agcactttgg gaggccgagg cgggcggatc acgaggtcaa gagattgagg tgaaaccccc 420
tctctactaa aagtagaaaa attagctggg catggtggtg cgtgcctgta atcccagcta 480
ctcaggagac tgaggcaggg aatctcttga acccaggagg cagaggttgc ggtgagccaa 540
gattgcacca cggcactcca gcctggtgac agagcgagac tccatctcaa aaaaagaaaa 600
aaaaaaaact gtcaaatgat actccaaaat ggttgtacca ttttatattt gcaacaacaa 660
tgtctgaggg tactgattgc tccatatcct tgacagcact tggtatagcc gatcctttaa 720
ttttaggcac tttaaggggg caaatacctg ggattttaaa ggtttaacct ttttattttc 780
ccaaatgggt taataggttc tcagcaactt ttcaaggggc ctaattcccc ccttcaaaat 840
aacctcccct gg 852

19

1854

DNA

BAC-F2 contig 44

19
ccggcactca ccgcggtggc ggccgctcta gaactagtgg atccccggaa atgttacttc 60
caacatttta gaactgaaat gattcttagt ctggtgataa atgtcaatta aaatagttct 120
cctttcacag agaaaattaa gaaaaaatta gttcaagaaa atatcaatca tgattgccag 180
cggaaatttg tttctgcagt aaaacaagca aaacaaatca aatccattaa aactagcaac 240
agactgtctt ctaaagtcaa gttcacatct ggagattttt ataaacttta ttggaaaagt 300
tctggttatc tatattttta gcatagcaaa atattcttct tgtttgttga atttgatata 360
aaatgttatt tttagccaag tcctggggca actcctacat ggctggaaaa tgttctcggt 420
gttaacaaag atgcaaagat cttaaatatt aatgttatca atcaactgga tactcttaag 480
tattatttgt aattatgtcc aatgtcatca ccacagggct gaccaacaag caaagagctg 540
acagtagtag caaaatgtag aaatctctgg taagcatgtt gtgtttatca atcctcttca 600
aatagatgaa attaaattgc atttaaagaa tgttacttat attaggcatt ttttgtgaaa 660
gacgttttaa actatggtgt cagaaaacag aaatactaaa cagaatgcat ttaacaggac 720
cttgaaatca ctgaatactc acctgtgtaa aagtcaaagt tcagataatt gaaatgttct 780
tactagtctc aagatgtctt ttggttacat agaaatttcc atgctgaatt ttgatttttt 840
taaaaagcca ttaatatgag tcaaaatcca ttatttcaca agtaaatgac ctttttatta 900
aaaaaaaaaa agagagagag agaagagcaa ggaaccaccc acatctaacc tcttaaatct 960
gagatcaata tatcaaaatt ttaatgtaca ttgaaaacat tttcatttta ttccacacac 1020
taccttttct tcataatttc ttattctgga catatagcag ttttttttgt cttttaaaac 1080
aggaaaaata aacaaacatg gtcttattat tgttactaag tcacaggtag taaagatggg 1140
accaggagaa ccttggagga ctagaaactt ctcaagagta gttagatttc acattcagag 1200
ggaggactca gagtcctgcc tgggacatac atttgcattc taggctcaag agcaaatatg 1260
tcagctttcc tttggtcaaa caatctttgc tacaggtcct aggtagttat atcagtggaa 1320
cctactaaag atgatggaat ttgtggtatt tcagggtagg aggtaaagtc ttagcaggct 1380
caactataca tgatcttaaa actaaatttg aaatgcagat gttctatgag ttagttggat 1440
attgtagtta tcccatctat caactgatca catttggtat gagcttgtta gttctgatta 1500
ggactcatct caacataata agaagggtgg catttagggc ccagtgtggg ggcctagtga 1560
tcactgctgg gacactgctt ctaaatcaac ataactaacc tctctaggat ggcaggctga 1620
ggctgctcaa gtacttcctg tctggcatct gggacagggc tgagtctctg ggtgggaaga 1680
tgggtgggag gactgaggct gatgagtata tgatataaat gagagccatt ggaatggctc 1740
cacatacagg acatgttgat aaatcatttt aacatatttt gctttctctc tctggtggcc 1800
cattgagaat caaaaggggg atccactagt tctagagcgg ccgccaccgc ggta 1854

20

1101

DNA

BAC-F2 contig 47

20
ccaccttttc aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa 60
tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag 120
attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac 180
ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata 240
taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca 300
cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga 360
gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac 420
tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt 480
tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc 540
tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg 600
cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg 660
ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa 720
gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag 780
agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt 840
agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac 900
aggatctgac attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt 960
agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca 1020
aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc 1080
aatttaatta tatcagttat t 1101

21

120

DNA

BAC-F2 contig 47 fragment 1

21
aactaatgta tcccccgggc tgcaggaaca cgatataaag ccttaaaatt gtgcgaatgt 60
grtaagtcga tccaatctca actgctatct rtgtaccaga atagtttcat aattacgtgt 120

22

300

DNA

BAC-F2 contig 47 fragment 2

22
gaattctctg wkattakaac tatcttgmct caaattsact tggtgagcta acctggcctg 60
tggtcccttg gctttaatgg aggctttgtc atatagatca tmtgtggtac tkgtgcctag 120
ttgtagtgcc ctgccttgct sttctwggct tactkgattt wggggtatac atcwatktaa 180
ytsaaaggtc tttctcctcc cgyygggaga atttctcctc ctccctcgga gaactctttc 240
tsccgaaatt ctattccggg ctgggtctcc attctgctta cctcccacac ttttaatmaa 300

23

599

DNA

BAC-F2 contig 47 fragment 3

23
gaattccctc ttgcttgggg gaggtcagcc ttttgttcta ttcaaatctt tgaggaaaat 60
agaaagcaaa gaatatatta actatattaa acaaactaaa tgttccaatt aaaatacaaa 120
aattataaag cctaataata aaagccctca attatatgct gtttaaaaga gacattttta 180
agcttaagga tatagaaaag ttgaaaataa aagaatggaa taaaataagc catgaaaata 240
ctagtataac actgatgtca aaatctgaca aagcacacaa aaaagaaaat aactttaact 300
gcaaaatctt aaaatcctag caaagaaaaa gcagcatatg ttataattat accacaacct 360
gatcaagtaa ggcttacttc aaaaatttaa ccatggtcca ttattggaaa acatattaat 420
aaaaatcctc acaaaaataa ttcaaaatat aaaaagccat atgataagcc tgatgaatgc 480
tggtttacag aactggtttt ctttaaaaag gcaatcattg gggaaataac ccgcttactc 540
agtatttact atgtgctagc cctgttcctt ctactagaaa ttagtgaaca aattctaac 599

24

330

DNA

BAC-F2 contig 47 fragment 4

24
aagctttcaa gaacagggac tgttaagccg ggtacagtgg ctcacaccta taatcctagc 60
attttgggag gccaaggcgg gtggatcact tgaggtcagg agttcaagac cagcctggcc 120
aacatggtga aaccccatct ctactaaaaa aaaaaaaaaa aaaaaaaaaa aaagaaatwc 180
maaaattacc caggcatggt ggcacgcgcc tgtaatccca kctacttggg aggctgaggc 240
aggaaaattg cttgaaccta ggaggcggag gtggcagtga cctaatcaca ccactgttct 300
ccatcctggg caacagaacg aaactgtttc 330

25

258

DNA

BAC-F2 contig 47 fragment 5

25
aagcttgggt gataatgagg agtcaatgtt ggtccatcaa ttgcaacaaa ggtaccacag 60
tggtgtagga tgtggataat gaggaggctg tgcacgtgtt ggggacaggt ggtatttacg 120
aatgctctat attttctttc tctctttttt taggacggag tctcactctg ttgcccacgc 180
tggaatgcay gggcatgact gtggctcact gtacccccca ctccccatgt tcaagagatt 240
ctcttgcctc acctcctg 258

26

622

DNA

BAC-F2 contig 47 fragment 6

26
ctcgagtcca ccgcggtggc ggccgctcta gaactagtgg atcccccgat ttatttaaag 60
cagttatgta tgtatgaaaa acaatgctga gcattcaatt ccaagatttc tgaagacacc 120
tattttacca tcactttgaa taaaattttt atattccttt cttcaaatac catctcggtt 180
ttcaaatgtg gctcattaaa tgtgaaagca aaatttcatt tcaaatagca gccttatcaa 240
atgacaattt acctgtggta gcattgttgg cactgacaca tatcagacca ctgccgagca 300
gaacaagaat gaaccaggaa tccatgctta tctggaaaat agggagtcat gttagatgag 360
gtcctatatt atcaggacta tgtctgagct ggtcaccaga agagtattct ggatttccaa 420
gctattaaaa tgtgtgccta aaccaatgat cttttgggag cctgatatgc atgcttcctc 480
agatatccaa taactaattg agtctttata aagactgact atcccttatc ttgaggacta 540
gcagtgtttc agattttttt taagagatag ggtcttgctc tgttgccagg atggagacag 600
tggttatgat catagctcag tg 622

27

602

DNA

BAC-F2 contig 47 fragment 7

27
tcggactcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg ccctcaggac 60
tgctgggctg cctggtgtca gcacttcccg ccattttcta tagcaccagt attattctta 120
atactttaaa aaaccaccag gcacggtggc tcacgcctgg aatcccagca ctttgggagg 180
ccaaggtggg cggatcacaa ggtcaggaga tcaagaccat cctggctaac acggtgaaac 240
cctgtctgta ctaaaaatag aaaaaaatta gctgggcgtg gtggcatgca cctgtagtcc 300
cagctgctgg ggaggctgag gcaggagaat ggcgtgaacc cgggaggcgg acttgcagtg 360
agccgagatt gcaccactgc actccagcct gggtgacaga gcgagacccc gtctcaaaaa 420
aaaaaagtaa ataaaaataa aaaaccatat cccactatct cccccttctc tctttgcctg 480
tgactannng gcatacttat ggggaaatct ttaagatgtc agatttcagt tctctcactt 540
ttctacaact tctccccatt ttgcctttct taggaacttc ccttcttccc atctgattcc 600
tn 602

28

546

DNA

BAC-F2 contig 47 fragment 8

28
tatcaaggcg gagtccacgg tggcggccgc tctagaacta gtggatcccc gaaccaggaa 60
tccatgctta tctggaaaat agggagtcat gttagatgag gtcctatatt atcaggacta 120
tgtctgagct ggtcaccaga agagtattct ggatttccaa gctattaaaa tgtgtgccta 180
aaccaatgat cttttgggag cctgatatgc atgcttcctc agatatccaa taactaattg 240
agtctttata aagactgact atcccttatc ttgaggacta gcagtgtttc agattttttt 300
taagagatag ggtcttgctc tgttgcccag gatggagaca gtggttatga tcatagctca 360
gtgcagcctc tacctcctgg actcaagtga tccttctgtc tcagcctcct gagtagctgg 420
gactataggc atgtactacg atgcctggct aatttttaaa attttctgta gagacggcgt 480
ctcactatgt tgtctaggct gctctcaaac tcttgggttc aactgatctc ttgcttcaac 540
ttccag 546

29

498

DNA

BAC-F2 contig 47 fragment 9

29
gtggattcag acgcggtggc ggccgctcta gaactagtgg atcccccgag cagaggttgc 60
agtgagccaa gatcgtgcta ctgtactcca gcctgggcaa cagagcaaga ctccgtctca 120
aaaaaaaaaa caaacaaacg atgtgtgcct gtgtttcctc atctgtagta tgaggataat 180
gatcatatat atttactagt gttgttggga tgatcaaatt aggtatattt aatcattgtg 240
taaaaaagtt gacgtgtaaa atccatgtaa aaaagttggc agaagagaca aactggtaaa 300
gcagccgttc ttcatttctc atttcattca acaagcatta ttaacagcct agcaagaaca 360
cagtatccag gaaaaatcaa agattatcaa gctcatgttc tataatcaag caatttataa 420
actagcagaa gaacaagaca gatgaataag aacttgggta tatttaaatg ctaagaagtt 480
caattcaaat aaatgtcc 498

Number	Date	Country	Kind
PO 6784	May 1997	AU
PO 8791	Aug 1997	AU

Number	Name	Date	Kind
5712134	Hadlaczky	Jan 1998
5721118	Scheffler	Feb 1998

Number	Date	Country
WO 9640965	Dec 1996	WO
WO 9808964	Mar 1998	WO

Nucleic acid molecules comprising a neocentromere

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