Claims
- 1. An isolated capsaicin receptor polypeptide.
- 2. The capsaicin receptor polypeptide of claim 1, wherein the polypeptide is a VR1 substype.
- 3. The capsaicin receptor polypeptide of claim 1, wherein the polypeptide is a VR2 subtype.
- 4. The capsaicin receptor polypeptide of claim 1, wherein the polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:2, 4, 9, 11, 23, 25, 27, 34, or 36.
- 5. An isolated polynucleotide sequence encoding a capsaicin receptor polypeptide of claim 1.
- 6. The polynucleotide sequence of claim 5, wherein the sequence encodes a VR1 subtype.
- 7. The polynucleotide sequence of claim 5, wherein the sequence encodes a VR2 subtype.
- 8. The isolated polynucleotide sequence of claim 5, wherein the sequence comprises a sequence selected from the group consisting of SEQ ID NO:1, 3, 5, 6, 7, 8, 10, 20, 21, 22, 24, 26, 33, or 35.
- 9. A recombinant expression vector comprising the polynucleotide sequence of claim 5.
- 10. A recombinant host cell containing the polynucleotide sequence of claim 5.
- 11. A method for producing the capsaicin receptor polypeptide of claim 1, the method comprising the steps of:
a) culturing a recombinant host cell containing a capsaicin receptor polypeptide-encoding polynucleotide sequence under conditions suitable for the expression of the polypeptide; and b) recovering the polypeptide from the host cell culture.
- 12. An isolated antibody that specifically binds a capsaicin receptor polypeptide of claim 1.
- 13. A method for identifying compounds that bind a capsaicin receptor polypeptide, the method comprising the steps of:
contacting a capsaicin receptor polypeptide with a test compound; and detecting specific binding of the test compound to the capsaicin receptor polypeptide.
- 14. The method of claim 13, wherein said detecting is by detecting of an alteration of intracellular calcium concentration in the capsaicin receptor-expressing host cell.
- 15. A method for detecting a vanilloid compound in a sample, wherein the vanilloid compound has activity in binding a capsaicin receptor polypeptide, the method comprising the steps of:
contacting a sample suspected of containing a vanilloid compound with a eukaryotic host cell expressing a capsaicin receptor polypeptide; detecting an alteration of a cellular response associated with capsaicin receptor activity in the capsaicin receptor-expressing host cell.
- 16. The method of claim 15, wherein the cellular response associated with capsaicin receptor activity is an increase in intracellular calcium concentration.
- 17. A pharmaceutical composition comprising a substantially purified capsaicin receptor polypeptide and a suitable pharmaceutical carrier.
- 18. A non-human transgenic animal model for capsaicin receptor gene function wherein the transgenic animal is characterized by having an alteration in capsaicin receptor function relative to a normal animal of the same species.
- 19. A method of screening for biologically active agents that modulate capsaicin receptor function, the method comprising:
combining a candidate agent with any one of:
(a) a mammalian capsaicin receptor polypeptide; (b) a mammalian capsaicin receptor-related polypeptide; (c) a cell containing a nucleic acid encoding a mammalian capsaicin receptor polypeptide; (d) a cell containing a nucleic acid encoding a mammalian capsaicin receptor-related polypeptide; or (e) a non-human transgenic animal model for function of a capsaicin receptor gene comprising one of:
(i) a knockout of a capsaicin receptor gene; (ii) an exogenous and stably transmitted mammalian capsaicin receptor gene sequence; or (iii) a capsaicin receptor promoter sequence operably linked to a reporter gene; and determining the effect of said agent on capsaicin receptor function
Priority Claims (1)
Number |
Date |
Country |
Kind |
PCT/US98/17466 |
Aug 1998 |
US |
|
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of 1) U.S. provisional patent application serial No.60/072,151, filed Jan. 22, 1998; and 2) U.S. patent application Ser. No. 08/915,461, filed Aug. 20, 1997; and 3) PCT international application PCT/US98/17466, filed Aug. 20, 1998, each of which applications are incorporated herein by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60072151 |
Jan 1998 |
US |
Continuations (2)
|
Number |
Date |
Country |
Parent |
09235451 |
Jan 1999 |
US |
Child |
09978303 |
Oct 2001 |
US |
Parent |
08915461 |
Aug 1997 |
US |
Child |
09978303 |
Oct 2001 |
US |