Claims
- 1. A compound according to the formula: ##STR39## wherein B is a nucleoside or deoxynucleoside base; wherein A is OH, H, halogen, SH, NH.sub.2, azide, OR.sub.2, SR.sub.2 or NR.sub.2 wherein R.sub.2 is a heteroatom substituted or unsubstituted blocking group;
- wherein R.sub.1 is a blocking group;
- wherein X is a secondary amino group of the formula NR.sub.6 R.sub.7 wherein R.sub.6 and R.sub.7 taken separately each represent a heteroatom substituted or unsubstituted alkyl, aryl, aralkyl, cycloalkyl, cycloalkylalkyl, alkenyl, cycloalkenyl, aralkenyl, alkynyl, aralkynyl or cycloalkynyl, R.sub.6 and R.sub.7 when taken together form an alkylene chain containing up to 5 carbon atoms in the principle chain and a total of up to 10 carbon atoms with both terminal valence bonds of the chain being attached to the nitrogen atom, and when R.sub.6 and R.sub.7 are taken together with the nitrogen atom to which they are attached to form a nitrogen heterocycle including at least one additional heteroatom from the group nitrogen, oxygen, and sulfur; and
- wherein M is sulfur single bonded to phosphorus and to R.sub.5 wherein R.sub.5 is a heteroatom substituted or unsubstituted alkyl, aryl, aralkyl, cycloalkyl, cycloalkylalkyl, alkenyl, cycloalkenyl, aralkenyl, alkynyl, aralkynyl or cycloalkynyl.
- 2. A compound according to the formula: ##STR40## wherein B is a nucleoside or deoxynucleoside base; wherein A is OH, H, halogen, SH, NH.sub.2, azide, OR.sub.2, SR.sub.2, or NR.sub.2 wherein R.sub.2 is a heteroatom substituted or unsubstituted blocking group;
- wherein R.sub.1 is a blocking group;
- wherein X is a secondary amino group of the formula NR.sub.6 R.sub.7, wherein R.sub.6 and R.sub.7 taken separately each represent a heteroatom substituted or unsubstituted alkyl, aryl, aralkyl, cycloalkyl, cycloalkylalkyl, alkenyl, cycloalkenyl, aralkenyl, alkynyl, aralkynyl or cycloalkynyl, R.sub.6 and R.sub.7 when taken together form an alkylene chain containing up to 5 carbon atoms in the principal chain and a total of up to 10 carbon atoms with both terminal valence bonds of the chain being attached to the nitrogen atom to which R.sub.6 and R.sub.7 are attached, and when R.sub.6 and R.sub.7 are taken together with the nitrogen atom to which they are attached form a nitrogen heterocycle including at least one additional heteroatom from the group of nitrogen, oxygen, and sulfur; and
- wherein M is sulfur single bonded to phosphorus and to R.sub.5 where R.sub.5 is a heteroatom substituted or unsubstituted alkyl, aryl, aralkyl, cycloalkyl, cycloalkylalkyl, alkenyl, cycloalkenyl, aralkenyl, alkynyl, aralkynyl or cycloalkynyl.
- 3. A compound according to claim 1 or 2 wherein R.sub.1 is a trityl group.
- 4. A compound according to claim 1 or 2 wherein R.sub.1 is a di-p-anisylphenylmethyl group.
- 5. A compound according to claim 1 or 2 wherein R.sub.1 is a p-anisyldiphenylmethyl group.
- 6. A compound according to claim 1 or 2 wherein M is sulfur single-bonded to phosphorus and to R.sub.5 where R.sub.5 is benzyl.
- 7. A compound according to claim 1 or 2 wherein M is sulfur single-bonded to phosphorus and to R.sub.5 is a substituted benzyl.
- 8. A compound according to claim 7 where M is sulfur single-bonded to phosphorus and to R.sub.5 where R.sub.5 is p-chlorobenzyl.
- 9. A compound according to claim 1 or 2 wherein M is sulfur single-bonded to phosphorus and to R.sub.5 where R.sub.5 is o,p-dichlorobenzyl.
- 10. A compound according to claim 9 wherein M is sulfur single-bonded to phosphorus and to R.sub.5 where R.sub.5 is heteroatom substituted lower alkyl.
- 11. A compound according to claim 10 wherein M is sulfur single-bonded to phosphorus and to R.sub.5 where R.sub.5 is .beta.-cyanoethyl.
- 12. A compound according to claim 1 or 2 wherein X is a secondary amino group, NR.sub.6 R.sub.7, where R.sub.6 and R.sub.7 are lower alkyl.
- 13. A compound according to claim 12 where X is diisopropylamino, dimethylamino, diethylamino and dibutylamino.
- 14. A compound according to claim 1 or 2 wherein B is adenine, guanine, cytosine uracil, and thymine.
- 15. The compound according to claim 2 wherein R.sub.1 is di-p-anisylphenylmethyl, B is thyminyl, M is p-chlorobenzylmercaptyl, A is H, and X is diisopropylamino.
- 16. The compound according to claim 2 wherein R.sub.1 is di-p-anisylphenylmethyl, B is 9-(N-6-benzoyladeninyl), M is p-chlorobenzylmercaptyl, A is H, and X is diisopropylamino.
- 17. The compound according to claim 2 wherein R.sub.1 is di-p-anisylphenylmethyl, B is 1-(N-4-benzoylcytosinyl), M is p-chlorobenzylmercaptyl, A is H, and X is diisopropylamino.
- 18. The compound according to claim 2 wherein R.sub.1 is di-p-anisylphenylmethyl, B is 9-(N-2-isobutyrylguaninyl), M is p-chlorobenzylmercaptyl, A is H, and X is diisopropylamino.
- 19. A compound according to claim 1 wherein X is selected from the group consisting of dimethylamino, diethylamino, diisopropylamino, dibutylamino, methylpropylamino, methylhexylamino, methylcyclohexylamino, ethylcyclopropylamino, methylbenzylamino, methylphenylamino, ethylchloroethylamino, methyltoluyamino, methyl-p-chlorophenylamino, methylcyclohexylmethylamino, bromobutylcyclohexylamino, methyl-p-cyanophenylamino, ethyl-.beta.-cyanoethylamino, morpholino, thiomorpholino, pyrrolidino, piperidino, 2,6-dimethylpiperidino and piperazino.
- 20. A compound according to claim 1 wherein X is diisopropylamino.
- 21. A compound according to claim 2 wherein X is selected from the group consisting of dimethylamino, diethylamino, diisopropylamino, dibutylamino, methylpropylamino, methylhexylamino, methycyclohexylamino, ethylcyclopropylamino, methylbenzylamino, methylphenylamino, ethychloroethylamino, methyltoluylamino, methyl-p-chlorophenylamino, methylcyclohexylmethylamino, bromobutylcyclohexylamino, methyl-p-cyanophenylamino, ethyl-cyanoethylamino, morpholino, thiomorpholino, pyrrolidino, piperidino, 2,6-dimethylpiperidino and piperazino.
- 22. A compound according to claim 2 wherein X is diisopropylamino.
- 23. A compound according to claim 1 where M is selected from a group consisting of ethylmercaptyl, methylmercaptyl, propylmercaptyl, butylmercaptyl, .beta.-cyanoethylmercaptyl, benzylmercaptyl, p-chlorophenylmercaptyl, p-chlorobenzylmercaptyl, 2,4-dichlorobenzylmercaptyl, cyclohexylmercaptyl, and p-nitrophenylethylmercaptyl.
- 24. A compound according to claim 1 where M is p-chlorobenzylmercaptyl.
- 25. A compound according to claim 2 where M is selected from a group consisting of ethylmercaptyl, methylmercaptyl, propylmercaptyl, butylmercaptyl, .beta.-cyanoethylmercaptyl, benzylmercaptyl, p-chlorophenylmercaptyl, p-chlorobenzylmercaptyl, 2,4-dichlorobenzylmercaptyl, cyclohexylmercaptyl, and p-nitrophenylethylmercaptyl.
- 26. A compound according to claim 2 where M is p-chlorobenzylmercaptyl.
Parent Case Info
This is a continuation-in-part application of earlier filed U.S. patent application No. 07/417,387, filed on Oct. 5, 1989, now abandoned, which in turn is a continuation in part of earlier filed U.S. patent application Ser. No. 07/314,011, filed on Feb. 22, 1989, now abandoned, which in turn is a continuation in part of U.S. patent application Ser. No. 07/198,886, filed on May 26, 1988, now abandoned.
Government Interests
The inventions described herein were supported, in part, with federal funds under a grant or award from the Department of Health, Education, and Welfare. Accordingly, the United States Government has certain statutory rights to the invention described herein under 35 U.S.C. 200 et seq.
US Referenced Citations (11)
Non-Patent Literature Citations (6)
Entry |
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Froehler, B. C. 1986, Tetrahedron Letters, vol. 27, No. 46, pp. 5575-5578. |
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Continuation in Parts (3)
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Number |
Date |
Country |
Parent |
417387 |
Oct 1989 |
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Parent |
314011 |
Feb 1989 |
|
Parent |
198886 |
May 1988 |
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