Claims
- 1. A DNA molecule comprising a single nucleotide sequence of hepatitis B virus, wherein said nucleotide sequence encodes a peptide of S protein, wherein said peptide is amino acids 128-132 of S protein of hepatitis B virus.
- 2. A DNA molecule comprising a single nucleotide sequence of hepatitis B virus, wherein said nucleotide sequence encodes a peptide of S protein, wherein said peptide is amino acids 127-132 of S protein of hepatitis B virus.
- 3. A DNA molecule comprising a single nucleotide sequence of hepatitis B virus, wherein said nucleotide sequence encodes a peptide of S protein, wherein said peptide is amino acids 126-132 of S protein of hepatitis B virus.
- 4. A fully double-stranded DNA molecule consisting of a nucleotide sequence encoding an antigenic site of S-protein of hepatitis B virus, wherein said antigenic site is amino acids 128-132 of S-protein and the serotype of said S-protein is selected from the group consisting of ayw, adw, adr, and ayr.
- 5. The DNA molecule of claim 4, wherein one strand of said nucleotide sequence is:
- CCT CAA GGA ACC TCT.
- 6. The DNA molecule of claim 4, wherein said DNA molecule is linked to DNA encoding a carrier molecule other than antigenic S protein.
- 7. The DNA molecule of claim 6, wherein said carrier molecule is .beta.-galactosidase.
- 8. The DNA molecule of claim 7, wherein said DNA molecule is linked to the lac operon.
- 9. The DNA molecule of claim 4, wherein said S-protein is of a hepatitis B virus serotype selected from the group consisting of adw, adr, and ayr.
- 10. The DNA molecule of claim 4, wherein said antigenic site is:
- Ala-Gln-Gly-Thr-Ser.
- 11. A fully single-stranded DNA molecule consisting of a nucleotide sequence encoding an antigenic site of S-protein of hepatitis B virus, wherein said antigenic site is amino acids 128-132 of S-protein and the serotype of said S-protein is selected from the group consisting of ayw, adw, adr, and ayr.
- 12. A fully double-stranded DNA molecule consisting of a nucleotide sequence encoding an antigenic site of S-protein of hepatitis B virus, wherein said antigenic site is amino acids 127-132 of S-protein and the serotype of said S-protein is selected from the group consisting of ayw, adw, adr, and ayr.
- 13. The DNA molecule of claim 12, wherein one strand of said nucleotide sequence is:
- ACT GCT CAA GGA ACC TCT.
- 14. The DNA molecule of claim 12, wherein said DNA molecule is linked to DNA encoding a carrier molecule other than antigenic S protein.
- 15. The DNA molecule of claim 14, wherein said carrier molecule is .beta.-galactosidase.
- 16. The DNA molecule of claim 15, wherein said DNA molecule is linked to the lac operon.
- 17. The DNA molecule of claim 12, wherein said S-protein is of a hepatitis B virus serotype selected from the group consisting of adw, adr, and ayr.
- 18. The DNA molecule of claim 12, wherein said antigenic site is:
- Thr-Ala-Gln-Gly-Thr-Ser.
- 19. A fully single-stranded DNA molecule consisting of a nucleotide sequence encoding an antigenic site of S-protein of hepatitis B virus, wherein said antigenic site is amino acids 126-132 of S-protein and the serotype of said S-protein is selected from the group consisting of ayw, adw, adr, and ayr.
- 20. A fully double-stranded DNA molecule consisting of a nucleotide sequence encoding an antigenic site of S-protein of hepatitis B virus, wherein said antigenic site is amino acids 126-132 of S-protein and the serotype of said S-protein is selected from the group consisting of ayw, adw, adr, and ayr.
- 21. The DNA molecule of claim 20, wherein one strand of said nucleotide sequence is:
- ACT ACT GCT CAA GGA ACC TCT.
- 22. The DNA molecule of claim 20, wherein said DNA molecule is linked to DNA encoding a carrier molecule other than antigenic S protein.
- 23. The DNA molecule of claim 22, wherein said carrier molecule is .beta.-galactosidase.
- 24. The DNA molecule of claim 23, wherein said DNA molecule is linked to the lac operon.
- 25. The DNA molecule of claim 20, wherein said S-protein is of a hepatitis B virus serotype selected from the group consisting of adw, adr, and ayr.
- 26. The DNA molecule of claim 20, wherein said antigenic site is:
- Thr-Thr-Ala-Gln-Gly-Thr-Ser.
- 27. A fully single-stranded DNA molecule consisting of a nucleotide sequence encoding an antigenic site of S-protein of hepatitis B virus, wherein said antigenic site is amino acids 126-132 of S-protein and the serotype of said S-protein is selected from the group consisting of ayw, adw, adr, and ayr.
Priority Claims (3)
Number |
Date |
Country |
Kind |
78 35588 |
Dec 1978 |
FRX |
|
79 21811 |
Aug 1979 |
FRX |
|
80 09039 |
Apr 1980 |
FRX |
|
Parent Case Info
This application is a continuation, of application Ser. No. 08/101,298, filed Aug. 3, 1993, now abandoned, which is a divisional of application Ser. No. 07/798,067, filed Nov. 27, 1991, now abandoned, which is a continuation of application Ser. No. 07/278,335, filed Dec. 1, 1988, now abandoned, said 07/278,335, filed Dec. 1, 1988, is a continuation of application Ser. No. 06/785,499, filed Oct. 8, 1985, now abandoned, which is a continuation-in-part of application Ser. No. 06/395,672, filed Jul. 6, 1982, now abandoned, which is a continuation of application Ser. No. 06/104,835, filed Dec. 18, 1979, now abandoned, AND a continuation of application Ser. No. 07/011,193, filed Feb. 5, 1987, now abandoned, which is a continuation of application Ser. No. 06/563,356, filed Dec. 20, 1983, now abandoned, which is a divisional of application Ser. No. 06/261,199, filed Apr. 30, 1981, issued as U.S. Pat. RE 34,705.
US Referenced Citations (5)
Non-Patent Literature Citations (5)
Entry |
Burrell et al., Nature, 279:43-47 (1979). |
Sninsky et al., Nature, 279:346-348 (1979). |
Galibert et al., Nature, 281:646-650 (1979). |
Valenzuela et al., Nature 280:815-819 (1979). |
Summers et al, Proc. Nat. Acad. Sci. USA, vol. 72, No. 11, pp. 4597-4601, Genome of Hepatitis B Virus: Restriction Enzyme Cleavage and Structure of DNA Extracted From Dane Particles, Nov. 1975. |
Related Publications (1)
|
Number |
Date |
Country |
|
011193 |
Feb 1987 |
|
Divisions (2)
|
Number |
Date |
Country |
Parent |
798067 |
Nov 1991 |
|
Parent |
261199 |
Apr 1981 |
|
Continuations (5)
|
Number |
Date |
Country |
Parent |
101298 |
Aug 1993 |
|
Parent |
278335 |
Dec 1988 |
|
Parent |
785499 |
Oct 1985 |
|
Parent |
563356 |
Dec 1983 |
|
Parent |
104835 |
Dec 1979 |
|
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
395672 |
Jul 1982 |
|