Claims
- 1. A purified nucleotide sequence comprising at least a part of the sequence coding for the urease protein expressed by Campylobacter pylori, wherein said part of a sequence is selected from the group consisting of sequence I, II, IIbis, V and VII.
- 2. A purified nucleotide sequence that specifically hybridizes with a nucleic acid probe having
- the following nucleotide sequence (I): ##STR10## or the following nucleotide sequence (IIbis): ##STR11## or the following nucleotide sequence (V): ##STR12## or the following nucleotide sequence (VII): ##STR13## or the nucleotide sequences complementary to the above-mentioned sequences (I), (II), (V) and (VII), respectively,
- wherein said purified nucleotide sequence specifically hybridizes with a probe sequence from C. pylori and not with a probe sequence from C. jejuni, C. fetus or C. coli.
- 3. A purified nucleic acid probe that specifically hybridizes with a nucleotide sequence encoding the urease expressed by Campylobacter pylori.
- 4. The nucleic acid probe of claim 3, wherein said probe is detectably labeled.
- 5. The nucleic acid probe of claim 3, wherein the length of said probe is about 25 to 40 nucleotides.
- 6. A kit for detecting the presence of Campylobacter pylori in a sample, said kit comprising:
- the nucleic acid probe of claim 3, and
- a medium suitable for hybridizing said probe to a nucleotide sequence to be detected.
- 7. A method of detecting Campylobacter pylori in a sample, said method comprising:
- contacting said sample with the nucleic acid probe of claim 3 under conditions sufficient to permit hybridization of said probe with a Campylobacter pylori nucleotide sequence, which encodes a protein having urease activity, and
- detecting the formation of the resulting hybridization complex, whereby the presence or absence of Campylobacter pylori is detected.
- 8. An isolated nucleotide sequence, which according to the universal genetic code, encodes the following amino acid sequence (III): ##STR14##
- 9. An isolated nucleotide sequence corresponding, according to the universal genetic code, to the following amino acid sequence (IV): ##STR15##
- 10. A nucleic acid probe that specifically hybridizes with a nucleotide sequence, said hybridization occurring under conditions of 68.degree. C. and 6.times.SSC in Denhardt medium, wherein said nucleotide sequence is selected from the group consisting of ##STR16##
- 11. The nucleic acid probe of claim 10, wherein said probe is detectably labeled.
- 12. The nucleic acid probe of claim 10, wherein the length of said probe is about 25 to 40 nucleotides.
- 13. A method of detecting Campylobacter pylori in a sample, said method comprising the steps of:
- contacting said sample with the nucleic acid probe of claim 10 under conditions sufficient to permit hybridization of said probe with a Campylobacter pylori nucleotide sequence that encodes a protein having urease activity, and
- detecting the formation of the resulting hybridization complex, whereby the presence of Campylobacter pylori is detected.
- 14. A method of detecting Campylobacter pylori as claimed in claim 13, wherein contacting said sample with said nucleic acid probe occurs at about 68.degree. C. and in 6.times.SSC in Denhardt medium.
- 15. A purified nucleotide sequence which hybridizes under stringent conditions of 68.degree. C. and 6.times.SSC in Denhart medium with the genes coding for Campylobacter pylori urease.
Priority Claims (1)
Number |
Date |
Country |
Kind |
88 13135 |
Oct 1988 |
FRX |
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Parent Case Info
This is a division of application Ser. No. 07/499,325, filed Jun. 5, 1990, filed as PCT/FR89/00518 Oct. 6, 1989.
Foreign Referenced Citations (1)
Number |
Date |
Country |
WO9633732 |
Oct 1996 |
WOX |
Non-Patent Literature Citations (4)
Entry |
Wetherall, B.L. et al, Med. Microbiol., 26: 257-263 (1988). |
Clayton et al., Infect. Immun. 57(2):623-629 (Feb. 1989). |
Clayton et al., Nuc. Acids Res. 18(2):362 (1990). |
Collins et al., J.Bact. 170(3):1041-1045 (Mar. 1988). |
Divisions (1)
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Number |
Date |
Country |
Parent |
499325 |
Jun 1990 |
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