Patent Application
20210177980
The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jun. 15, 2017 is named 204257_0028_561478_SL_ST25.txt and is 45,280 bytes in size.
Administering interferon alpha (INF-α) has medical sequelae that need to be assessed and managed. For example, autoimmunity can also be induced by INF-α therapy for chronic viral hepatitis in humans. Therefore, generally human treatment has been limited to patients in need of therapy, as therapy can aggravate pre-existing auto-immunity, unmask silent autoimmune processes, or even induce de novo autoimmune diseases. F. L. Dumoulin et al., “Autoimmunity induced by interferon-α therapy for chronic viral hepatitis,” Biomed. & Pharmacother., 53: 242-54 (1999). Interferon-α also been observed to fail to treat Hepatitis C virus infections in humans.
Interferon has been seen to be effective to treat some humans with hepatitis C virus (HCV), to the extent that two pegylated forms of interferon alpha (INF-α) now are in clinical use for humans, i.e. Peginterferon-α-2a and Peginterferon-α-2b, known also as PEGASYS® and PEGINTRON® respectively. Yet, it remains unclear how INF-α inhibits HCV replication. More interestingly, Peginterferon-α-2a and Peginterferon-α-2b have only 7% and 28% activity respectively as compared to their native (wild-type) forms.
In the process of making interferon, various variants have been created for human treatment to extend bioavailability and assist in protein production. One example is in U.S. Pat. No. 8,106,160, which discusses addition of one or more amino acid residues to the N-terminal cysteine of mature human interferon alpha-2b to reduce the formation of non-natural disulfide bonds and thereby lowering the level of structural isoforms. This includes the addition of a proline residue at the N-terminus.
Methods for introducing non-natural amino acids inserted into sites in a protein are described for example in WO2010/011735 and in WO2005/074650.
The wild-type porcine interferon alpha-1 is 189 amino acids in length and is located at GenBank X57191
Forms of interferon-α for use in animals and in animal husbandry are needed, especially in treating pig populations susceptible to viral infections, and even more particularly in treating pig populations with active and on-going viral infections to protect pig herds from pathology associated with viral infection. A benefit would be finding an IFN-α variant for use in porcine animals that is long acting and useful to inhibit or reduce viral replication, herd pathology and animal death related to virus infection. The porcine IFN-α variant would maintain bioactivity, have a longer bioavailability and have few isoforms allowing for easier purification.
Provided here are porcine interferon-α (pINF-α) variants and methods of use thereof that can be used in animals that are not vaccinated, such as unvaccinated newborn piglets as well as in immune suppressed animals, i.e. pregnant pigs (pregnant sows or gilts); in animals where vaccination confers insufficient protection; in animals susceptible to infection by a virus for which no effective vaccine is available; and in currently-infected animals. These compositions and methods of use will be to prevent infection in the fact of a viral outbreak. The compositions can also be used to reduce the severity of disease in an infected pig. Generally, a single dosage regimen is administered. Alternatively, and as needed, dosages can be provided approximately 1-3, or 2 weeks apart, with optimally only one to two dosages administered
The wild type porcine interferon-α (pINF-α) which includes the 23 residue signal sequence is:
maptsaflta lvllscnaic slgcdlpqth slahtralrl
The double underlined portion is the signal sequence which is not present in the mature form of wild type pIFN-α.
Provided here is a (pINF-α) variant. The variant omits the 23 amino acid signal sequence. The variant can have a methionine residue at the amino terminal end which may be cleaved off in the mature form of the protein. This methionine is not present in the wild-type form of pIFN-α. The pINF-α variant further has a one or two amino acid insertion between a signal sequence methionine and the N-terminal cysteine. The insertion is either a proline or a proline-serine. The sequence is depicted below: MXaXbCDLPQTHSLAHTRALRLLAQMRRISPFSCLDHRRDFGSPHEAFGGNQVQKAQAM ALVHEMLQQTFQLFSTEGSAAAWNESLLHQFYTGLDQQLRDLEACVMQEAGLEGTPLL EEDSIRAVRKYFHRLTLYLQEKSYSPCAWEIVRAEVMRSFSSSRNLQDRLRKKE (SEQ ID NO: 2). “Xb” (position 3 in SEQ ID NO: 2) is either Proline, Serine or Absent. Xa (position 2 in SEQ ID NO: 2) is either absent or proline. When Xb is absent, Xa is absent, when Xb is proline Xa is absent, and when Xb is serine, Xa is proline. The bold glutamic acid residue “E” located downstream of Xa and Xb is the site of substitution of a synthetic amino acid, i.e. pAF.
The pINF-α variant further has the synthetic amino acid, para-acetyl-phenylalanine (pAF), substituted in one of 5 locations in SEQ ID NO: 1: residue E103 (SEQ ID NOS: 9 to 11 respectively), E107 (SEQ ID NOS: 12 to 14 respectively), L112 (SEQ ID NOS: 15 to 17 respectively), Y136 (SEQ ID NOS: 18 to 20), or Q102 (SEQ ID NOS: 6 to 8 respectively) (numbering is with respect to the sequence shown in SEQ ID NO: 4 without an N-terminal Met).
The pINF-α variant can further be pegylated on the pAF. The pINF-α variant can be pegylated with about a 5 to about a 40 kDa PEG, or with a 30 kDa PEG. The pINF-α variant can be pegylated with a linear 30 kDa oxyamino-PEG. An activated oxyamino group reacts chemoselectively with an acetyl side chain present on a synthetic amino acid, such as para-acetylphenyalanine. A pINF-α variant has a linear 30 kDa PEG covalently bonded to the pAF substituted at E107 (SEQ ID NOS: 12 to 14) (numbering is with respect to mature sequence shown in SEQ ID NO: 4). The E107 pINF-α variant can further have a proline or proline and serine at the N-terminus as reflected in SEQ ID NOS: 13 and 14 respectively.
The pINF-α variants can be incorporated into formulations. A formulation comprising the pINF-α variants can comprise 20 mM sodium acetate, 100 mM sodium chloride, 5% glycerol at pH 5.0 of 2.0 to 6.0 g/L titer of porcine INF-α.
The formulations can be made by a method comprising the steps of:
The pINF-α variants and formulations thereof can be used to treat a virus infection. A method of treating a virus infection in a pig comprising administering the pINF-α variant subcutaneously to a pig in the amount of 25 μg/kg to 150 μg/kg animal weight of any of the porcine interferon-α (pINF-α) variants. The method can further comprise a second administration of about 25 μg/kg to about 150 μg/kg animal weight of the pINF-αA variant. If the method involves a second administration, the second administration can occur about 7, 14, or 21 days after the first administration.
The method of treating virus infection can be used to treat a virus infection selected from the group consisting of: porcine reproductive and respiratory disease virus, foot and mouth disease virus, swine influenza virus, porcine circovirus, porcine epidemic diarrhea virus, and transmissible gastroenteritis virus. The method can be used to treat a newborn pig or a pregnant pig.
Also contemplated is a use of any of the pIFN-α variants (or a formulation containing the variant) in a therapy in a pig to treat a virus infection, wherein the infection can be caused by any of the viruses discussed herein.
Also described is a use of the pINF-α variant for use in the manufacture of a medicament for treating a viral infection in a pig. The pig can be a newborn pig or a pregnant pig.
By “administering” is meant the injection of a therapeutically effective amount of the compounds and compositions containing said compounds disclosed. Administration can be intramuscular (i.m) or subcutaneous (s.c.). The amount of pINF-α variant administered would be given based on the weight of the animal, for example with pregnant pigs receiving more than newborn pigs.
The formulations disclosed herein can also be placed in a single dose or a multi-dose vial.
The pig being treated by the methods described herein would be a newborn pig, r a pregnant pig (pregnant gilt or sow).
By “treat”, “treating”, or “treatment” is meant the reduction or amelioration of one or more symptoms associated with infection by one of the viruses mentioned herein. The composition can further be used in the manufacture of a medicament for treating a viral infection in a pig. The composition or medicament can be used to treat a pig. The pig can be a newborn pig or a pregnant pig.
Reference will now be made in detail to the compounds, formulations of said compounds, methods of making the formulations, and methods of using the compounds and formulations to treat pigs (porcines or swine) having a viral infection.
As used herein and in the appended claims, the singular forms “a,” “an,” and “the” include plural reference unless the context clearly indicates otherwise, and plural usage in turn can also encompass usage in the singular.
A “synthetic amino acid” refers to an amino acid that is not one of the 20 common amino acids or pyrrolysine or selenocysteine. Examples of such synthetic amino acids include, but are not limited to, para-acetyl phenylalanine (pAF), acetylglucosaminyl-L-serine, and N-acetylglucosaminyl-L-threonine. For additional details on such synthetic amino acids and their incorporation and modification, see WO2010/011735 and in WO2005/074650.
The term “subject” as used herein, refers to a pig, especially the domestic pig (Sus scrofa domesticus or Sus domesticus) and can include miniature pigs as well as those breeds raised for meat production. By “pig”, “swine” or “porcine” is meant to include all pig breeds.
The term “effective amount” as used refers to that amount of the pINF-α variant being administered which will prevent, treat, or reduce the transmission of a porcine virus from an infected animal to an uninfected animal or will prevent, treat, or reduce a symptom of a disease caused by infection with a porcine virus. Disclosed here are porcine interferon alpha (pIFN-α) variants (pIFN-α). These variants have had synthetic amino acids substituted at various positions on a porcine IFN-α sequence. A Sus scrofa domestica pIFN-α gene (GenBank X57191) wherein the underlined leader sequence (residues 1-23 of SEQ ID NO: 3) is removed as depicted below (amino to carboxy terminus orientation):
MAPTSAFLTALVLLSCNAICSLGCDLPQTHSLAHTRALRLLAQMRRISPF
The mature sequence is: CDLPQTHSLAHTRALRLLAQMRRISPFSCLDHRRDFGSPHEAFGGNQVQKAQAMALVH EMLQQTFQLFSTEGSAAAWNESLLHQFYTGLDQQLRDLEACVMQEAGLEGTPLLEEDS IRAVRKYFHRLTLYLQEKSYSPCAWEIVRAEVMRSFSSSRNLQDRLRKKE (SEQ ID NO: 4). The underlined signal sequence at the N-terminus of SEQ ID NO: 3 can be replaced by another signal sequence or even with a single methionine residue, for example for in vitro transcription, resulting in the following sequence: MCDLPQTHSLAHTRALRLLAQMRRISPFSCLDHRRDFGSPHEAFGGNQVQKAQAMALV HEMLQQTFQLFSTEGSAAAWNESLLHQFYTGLDQQLRDLEACVMQEAGLEGTPLLEED SIRAVRKYFHRLTLYLQEKSYSPCAWEIVRAEVMRSFSSSRNLQDRLRKKE (SEQ ID NO: 5) or can include the proline or proline-serine residues between the N-terminal methionine and cysteine (see SEQ ID NO: 2). Other related porcine sequences can be used.
The pIFN-α variants have had synthetic amino acids introduced into one of the following locations on Sus scrofa domestica (using its numbering with respect to the mature sequence shown in SEQ ID NO: 4): Q102 (SEQ ID NO: 6), E103 (SEQ ID NO: 9), E107 (SEQ ID NO: 12), L112 (SEQ ID NO: 15), and Y136 (SEQ ID NO: 18) (which are bolded and underlined above) and also depicted in
Synthetic amino acids and their incorporation are as discussed for example in WO2010/011735. Synthetic amino acids can be used in Escherichia coli (E. coli) (e.g., L. Wang, et al., (2001), Science 292: 498-500) and in the eukaryote Saccharomyces cerevisiae (S. cerevisiae) (e.g., J. Chin et al., Science 301: 964-7 (2003)), which has enabled the incorporation of synthetic amino acids into proteins in vivo.
Of the five (5) listed synthetic amino acid substitution variants having biological activity (substitutions of pAF at one of the five E103, E107, L112, Y136, or Q102 residues of which are underlined in the sequence below), these variants can be further modified by having amino acids inserted at the N-terminus of the molecule. For example, a proline (Pro) or Proline-Serine (Pro-Ser) can be inserted between the N-terminal Met and Cys.
The mature form of the variants would omit the N-terminal methionine.
Methods of making a pINF-α polypeptide linked to a water soluble polymer are described here. By “pegylating” and “pegylated” is meant to refer to the covalent bonding of the specified synthetic amino acid to a polyethylene glycol (PEG) molecule. The method can comprise contacting an isolated pINF-α polypeptide comprising a synthetic amino acid with a water soluble polymer comprising a moiety that reacts with the synthetic amino acid.
The poly(ethylene glycol) molecule can have a molecular weight of between about 0.1 kDa and about 100 kDa. The poly(ethylene glycol) molecule can have a molecular weight of between 0.1 kDa and 50 kDa, 20 kDa and 40 kDa, and any integer value between 25 kDa and 35 kDa. The poly(ethylene glycol) molecule can have a molecular weight of about 30 kDa. The poly(ethylene glycol) molecule can be a linear molecule having a molecular weight of between 0.1 kDa and 50 kDa, 20 kDa and 40 kDa, and any integer value between 25 kDa and 35 kDa. The poly(ethylene glycol) molecule can be a linear molecule having a molecular weight of 30 kDa. The poly(ethylene glycol) molecule can have an aminooxy group capable of reacting with an acetyl group on a synthetic amino acid. The poly(ethylene glycol) molecule can be a 30 kDa aminooxy activated linear PEG capable of forming an oxime bond with the acetyl side chain of para-acetylphenylalanine.
One pINF-α has a linear PEG that is about 30 kDa attached to the pAF residue.
The variants discussed above and further below can be further mixed into a formulation with various excipients, stabilizers, buffers, and other components for administration to animals. Identifying suitable formulations for stability, animal administration, and activity are not simple matters. Instead, the formulation must be tailored to each compound, the needs for purifying that compound, the stabilizer needed to maintain the compound, and various other formulation components.
Suitable salts for inclusion into the formulation include sodium chloride, potassium chloride or calcium chloride.
Sodium acetate can be used as a buffering agent and/or a stabilizing agent.
Suitable buffers can include phosphate-citrate buffer, phosphate buffer, citrate buffer, L-histidine, L-arginine hydrochloride, bicarbonate buffer, succinate buffer, citrate buffer, and TRIS buffer, either alone or in combination.
The formulation can also include a cryoprotectant. Cryoprotectants can include a cryoprotectant selected from the group consisting of hydroxypropyl-β-cyclodextrin (HPBCD), sucralose, and polyvinylpyrrolidone 4000 (PVP 4000).
The formulation may optionally include a surfactant. Suitable surfactants include polysorbates (e.g., polysorbate 80), dodecyl sulfate (SDS), lecithin either alone or in combination.
The formulation can be an aqueous composition or in the form of a reconstituted liquid composition or as a powder. The formulation can further stored in a vial or cartridge or in a pre-filled sterile syringe for ready administration to a subject.
The pH of the formulation can range from 4.0 to 7.0 or 4.5 to 6.5 when the formulation is in a liquid form.
Suitable viral infections that can be treated include without limitation a coronavirus, a pestivirus (swine fever virus or classical swine fever, CSF), transmissible gastroenteritis coronavirus (TGEV), swine arterivirus (PoAV), a porcine reproductive and respiratory syndrome virus (PRRSV), a porcine circovirus (PCV), a porcine epidemic diarrhea virus (PEDV), foot and mouth disease virus (FMDV), porcine coronavirus such as porcine deltacoronavirus (PDCoV), and swine influenza virus (SIV).
The administration of the variant or a formulation containing the variant can be a single dose or single dose followed by a secondary dosage 7 to 21 days after the first dose, e.g. about 14 days after the first dose. The animal is administered the variant in an amount of variant of about 25 μg/kg to about 150 μg/kg of variant per kg animal weight. Another effective range amount of the pINF-α variant is about 50 μg/kg to about 100 μg/kg animal weight. The variant can be administered in a formulation or by itself. The variant can be administered once for example prior to an outbreak. The variant can also be administered after a virus outbreak to the herd to prevent further herd loss. The variant or a formulation thereof can be administered a second time. The second administration can be administered about 7 to about 21 days after the first administration, for example 14 days after the first administration. Further administrations may be contemplated if needed to reduce or prevent disease associated with viral infections.
It will be apparent to those skilled in the art that various modifications and variation can be made without departing from the spirit or scope of the compositions, compounds, and methods disclosed herein. Thus, it is intended that the present invention cover the modifications and variations of this invention provided they come within the scope of the appended claims and their equivalents.
Eleven (11) pAF variants are created and compared against the wild-type pINF-α (wt). Of these, three pINF-α variants are dropped from continued testing because of poor protein expression/production issues for the synthetic amino acids substituted at various points in the pINF-α.
AXID2820 has plasmid pKG0083 with pIFN-α-E107amber and the proline-serine N-terminal insertion (
The eight (8) pINF-α variants in Table 1 were capable of protein expression. These eight variants are also pegylated with a 30 kD aminooxy activated linear PEG. Pegylation of the variants is accomplished by adjusting the protein solution (at a protein concentration of approximately 20 mg/mL or greater) to pH 4.0 with 10% glacial acetic acid. Acetic hydrazide is added to a final concentration of 100 mM and oxyamino PEG is added in a molar ratio of between 1:1 and 2:1, or up to about 8:1, relative to the pINFα variant. The reaction is allowed to proceed for 1-3 days at 28-30° C. in the dark. The reaction is quenched by diluting the reaction 5-fold with 30 mM sodium acetate, pH 5.0. These 8 pegylated variants are analyzed using size exclusion chromatography (SEC), as shown in Table 1. The pINF-α variants are the same as the wild-type pINF-α except for a pAF substitution at the indicated residue, e.g., His7 was substituted with pAF, Arg34 was substituted with pAF and so forth. Protein concentration is indicated at mg/mL. “RP” stands as Reverse Phase (i.e. reverse phase chromatography, or RP-HPLC).
SEC is accomplished using an HPLC system capable of multiple wavelength detection (Agilent 1100 or 1200, or equivalent). The mobile phase is 200 mM potassium phosphate, 250 mM potassium chloride, pH 6.0, 10% isopropanol (IPA).
These 8 variants are tested in an in vitro bioactivity assay. The commercially available interferon assay kit iLite™ huIFNα kit by Pestka Biomedical Laboratories, Inc. (Piscataway, N.J., USA) is used to test the biological activity of pIFN-α. The fold loss in activity of the PEG-variants is calculated relative to Ambrx WT protein as follows:
As shown in Table 2, of the eight tested, three of the pINF-α variants had substantially less activity than wild-type unpegylated pINF-α:
The wild-type pIFN-α control lacks the signal peptide, has no inserted N-terminal amino acids (Methionine, Proline or Serine), has no pAF substitution for any amino acid, and is not pegylated (SEQ ID NO: 4).
As a result of these experiments, the pegylated pIFN-α variants having a pAF substitution at H40 (SEQ ID NO: 23), H7 (SEQ ID NO: 21), and R34 (SEQ ID NO: 22) are deemed not useful pIFN-α variants for treating viral infection in porcines given their low activity levels as compared to the wild type in the iLite™ huIFNα assay.
RP-HPLC analysis of both the pegylated and unpegylated variants for the results in Table 2 use a mobile phase A (0.05% TFA/water) and a mobile phase B (0.05% TFA/ACN).
Eighteen Sprague Dawley rats (n=3 group) are dosed at 0.2 mg/kg of each of 6 test samples (resuspended in 20 mM sodium acetate, pH 5.0, 100 mM NaCl, and 5% glycerol) subcutaneously in the scruff of their necks, distal to the catheter: (1) pIFNα-E103-PEG30K (pegylated SEQ ID NO: 9) (2) pIFNα-L112-PEG30K (pegylated SEQ ID NO: 15), (3) pIFNα-E107-PEG30K (pegylated SEQ ID NO: 12), (4) pIFNα-Y136-PEG30K (pegylated SEQ ID NO: 18), and (5) pIFNα-a-Q102-PEG30K (pegylated SEQ ID NO: 6).
The pIFN-α variants are solubilized in 20 mM sodium acetate at pH 5.0, 100 mM NaCl and 5% glycerol. Each animal is injected subcutaneously (i.e., intranuchally) with either one of the 5 variants or the wild type pIFN-α (the same as that used in Example 1) thus forming the 6 test groups of 3 rats each.
The sampling time points are: pre-dose and post dose at 1 hr, 6 hr, 24 hr, 48 hr, 72 hr, 144 hr, 192 hr, and 240 hours post dosing. Samples of blood are obtained via the jugular vein catheter or lateral tail vein or at the termination of the experiment via cardiac puncture and are processed by allowing the blood to clot and removing the resulting serum. The concentrations of each pegylated pIFN-α variant are determined using a ligand binding assay using an anti-PEG monoclonal capture antibody and a goat anti-porcine IFN-α polyclonal detection antibody. AUClast, Cmax, Tmax are calculated by analysis of the raw data using WINNONLIN® PK modeling software (Pharsight Corporation, now Certara USA, Inc.). The exposure (AUClast) of the pegylated variants was divided by the exposure of the wild type pIFN (WT pIFN) to obtain fold differences. The results for the pegylated variants are as follows in Table 3:
As evidenced, exposure (in terms of AUClast) is highest for PEG30K-Q102 and PEG30K-E107, and as presented in the table in decreasing order of exposure. All of the five tested pIFN-α variants have a higher exposure for pIFN-α than the wild type pIFN-α form (SEQ ID NO: 4). Tmax is generally observed at 24 hrs post-dose for pegylated pIFN-α variants as compared to 1 hour post-dose for wild type pIFN-α. For this experiment, Cmax is included in the half-life calculation due to insufficient data points in the terminal phase. As time at Cmax does not represent the true elimination phase, half-life estimates should be considered as an approximation.
It should be noted that the species heterology has no impact in this type of study.
Based on mass spectroscopy (MS) of pIFN-αA-E107 (SEQ ID NO: 12), there are acetylated forms, a 58 Da form and oxidation contaminants. The variant becomes acetylated during biosynthesis inside the E. coli production strain. Thus, one option for pIFN-αA-E107 production is to add acetyltransferases at the appropriate production time or to use knockout ribosomal-protein-alanine acetyltransferase (RimJ) (N-terminal acetyltransferase). Additionally, small and large scale chromatography can be used for purification. Alternatively and as shown herein, the N-terminus sequence can be further modified to prevent acetylation from occurring on the variant altogether.
For chromatography means, one method is to use CAPTO Adhere Impres (GE Healthcare Lifesciences), which is a strong anion exchange multimodal BIOPROCESS chromatography medium (resin). This method is used with a mobile phase of 25 mM ammonium acetate (at pH 6.5), loading the unmodified (i.e. not pegylated) pIFN-α variant to a concentration of 1-5 mg/mL resin. The column is washed with 5 column volumes (CV) 25 mM ammonium acetate at pH 6.5. Elution of the pIFN-αA variant is with a linear gradient to 100% elution buffer (5 mM acetic acid), and 0-100% B over 40 CV, whereby the oxidized pIFN-α peaks are removed. Peak One has an N-terminal pIFN-α without cysteine acetylation, whereas Peak 2 contained an acetylated form (+42 Da), a +58 Da form, a +58+1 Ox form (likely an acetylated and singly oxidized species), and a +58 Da+2 Ox form (likely an acetylated form with at least two oxidations).
Because of these results, a proline was inserted at the amino terminus of pIFN-α to prevent the acetylated forms. Thus a Pro-pIFNα-E107pAF (SEQ ID NO: SEQ ID NO: 13) was created. For clarity, numbering of amino acid positions always begins with the N-terminal cysteine (C1). The added proline becomes residue −1, and the N-terminal methionine (if any) becomes residue −2. A Pro-Ser insertion can also be made, wherein the peptide contains serine at the −1 position, proline at the −2 position, and possibly a methionine at the −3 position relative to the N-terminal cysteine. The addition of the proline removed the other peaks seen for the prior variant when analyzed via mass spectroscopy.
In Table 4 below, the Pro-pIFNα-E107pAF has a proline at the N terminus and a pAF substitution on E107. The activity of the Pro-pIFNα-E107pAF variant is compared to the activity of the variant lacking the addition of proline at the N-terminus, pIFNα-E107pAF. The variant having the added proline has fewer acetylated and oxidized variants. Note these variants are not pegylated and they do not include the signal sequence methionine.
Given the success with the proline addition to the N-terminus of pIFNα-E107pAF, other N-terminal variants were also assessed including a Serine (Ser, S) addition, a Pro-Ser (PS) addition, a His (H) addition, and a Ser-Gly (SG) addition to pIFNα-E107pAF. These N-terminal variants are not pegylated. The activity of these N-terminal variants (all are E107-pAF variants) was then assessed in the in vitro bioassay using iLite™ huIFNα kit by Pestka Biomedical Laboratories described above and the results are presented in Table 5.
Pro-pINFα is the variant having the proline added at the amino terminus between the methionine (in the immature peptide) and the cysteine.
The pINFα-PS-E107 variant (SEQ ID NO: 14) is taken from the Capto chromatography pool after using Capto chromatography as per manufacturer instructions and 0.2 M glycine is added to the purified form. The pH of the mixture is adjusted to 4.0 with acetic acid. The pINF-α variant is then concentrated to 8.2 mg/mL using an Amicon Ultra centrifugal filter according to manufacturer's instructions. Once concentrated, 30K linear PEG (PEG can be purchased commercially from NOF America Corporation or EMD Merck for examples) is added in a 8:1 molar ratio of pEG:pINFα variant. The PEG/pINF-α variant mixture is then incubated at 28° C. for about 18 hours. This method results in >95% of the pINF-α variant being conjugated with PEG after 18 hours of incubation.
The pegylated variant (pINFα-PS-E107-PEG30K) can then be purified as follows. A 143 mL SP650S Tosoh column can be used to purify the pegylated variant using a mobile phase of:
A: 30 mM sodium acetate, pH 5.0
B: 30 mM sodium acetate, 5% ethylene glycol, pH 5.0
0 to 100% B over 20 column volumes.
Activity assays are run for several variants in both their pegylated (30 KDa linear PEG) and unpegylated forms to assess the impact of pegylation on the variants having pAF substituted at E107 and having proline-serine at the amino terminus (SEQ ID NO: 14). The results are provided below in Table 6. Protein concentration, SEC, RP, and EC50 values are determined as discussed above. The pIFNα-P-E107-pAF is used as a comparator sample to reflect the results for protein without an amino terminal extension.
While testing the characterization of the variants, norleucine was observed to be misincorporated. Norleucine is known to be misincorporated at the amino acid methionine in high density fermentation with E. coli. This was observed in the fermentation runs performed. Norleucine incorporation was reduced by using one or more of the following steps: feeding the solutions with methionine, fermenting with complex media versus defined media (the complex media has one or more non-defined components in it including but limited to glycerol, salts, amino acids, vitamins, yeast extracts, plant and animal hydrosolates, peptones, and tryptones), and/or lowering the temperature of the reaction mixture post induction. L-methionine is added to the batch medium at a concentration of 1.2 mM as well as fed continuously via the feed solution which contains 20 mM L-methionine.
The pINF-α variants are under the control of a T7 promoter. Addition of arabinose (the inducer) to the fermentation results in a cascade which enables production of the variants. Thus, post-induction means after the inducer, in this case arabinose, is added.
Samples were frozen and thawed over five cycles by freezing at 0° C. in 1.5 mL tubes and thawing in a room temperature water bath. No significant impact was observed for the high molecular weight (HMW) protein profile over the five cycles of freeze-thawing as evidenced below in Table 7:
Table 7 reflects the differences between the pegylated and unpegylated variants having the proline-serine at the N-terminus as well as the pAF substituted at E107, wherein the variant has the proline-serine insertion at the amino terminus (pIFNα-PS-E107-30), i.e. at residues −2 and −1.
The present application is a continuation of U.S. patent application Ser. No. 16/311,540 filed Dec. 19, 2018; which is a 35 USC 371 application of PCT/US17/037964 filed Jun. 16, 2017; which claims priority to the benefit of U.S. Provisional Patent Application Ser. No. 62/352,163 filed Jun. 20, 2016, the disclosure of which is hereby incorporated by reference in its entirety.
| Number | Date | Country | |
|---|---|---|---|
| 62352163 | Jun 2016 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 16311540 | Dec 2018 | US |
| Child | 17186642 | US |
