PEPTIDE INHIBITORS OF CD40L SIGNALING AND USES THEREFOR

Abstract
The present invention provides compositions comprising peptidyl inhibitors of CD40L-dependent signaling that are not derived from a natural binding partner of CD40L such as CD40, or from a native CD40-CD40L interface. More particularly, the peptidyl inhibitors of the present invention are derived from natural sources that do not express CD40-Cd40L costimulatory pathways. The invention also provides synthetic derivatives and analogs of the peptidyl inhibitors having enhanced binding affinity for CD40L or enhanced inhibitory activity relative to their parent molecules.
Description
FIELD OF THE INVENTION

The present invention relates to peptide-based compositions, analogs thereof and their use in medicine, for example in a method of diagnosis and/or prognosis and/or therapy of the human or animal body or in an ex vivo method of diagnosis and/or prognosis and/or therapy of the human or animal body.


BACKGROUND
Protein-Protein Interactions

The majority of biological processes in living organisms are mediated by proteins and their interactions with specific ligands e.g., other proteins, antigens, antibodies, nucleic acids, lipids and carbohydrates. Not only are such interactions involved in normal biological processes, protein interactions are also causative of processes involved in diseases or disorders. As a consequence, protein interactions are important targets for the development of new therapeutic compounds.


CD40 ligand (CD40L or CD154) and CD40L signaling effects


CD40 ligand is a trimeric, transmembrane protein of the tumor necrosis factor family. A large variety of immunologic and vascular cells have been found to express CD40, CD40 ligand, or both.


For example, the CD40 ligand (CD40L or CD154), which is not expressed on resting human T cells, is up-regulated on the T-cell surface in response to foreign antigen presentation on MHC-class II molecules, up-regulation of the B7 antigen on the B-cell surface, formation of a complex between T-cells and B-cells via the T-cell receptor (TCR), and antigen recognition. Stimulation through the TCR also activates the T-cells, initiating T-cell cytokine production, interaction between the CD28 antigen on T-cells and the B7 antigen on B cells and binding of CD40L to CD40 receptor on the B-cell surface to thereby stimulate the B-cell to mature into a plasma cell secreting immunoglobulin.


The interaction between CD40L and the CD40 receptor may also cause adverse effects and transformed cells from patients with low-grade and high-grade B-cell lymphomas, B-cell acute lymphoblastic leukemia, multiple myeloma, chronic lymphocytic leukemia, and Hodgkin's disease express CD40. CD40 expression is also detected in two-thirds of acute myeloblastic leukemia cases and 50% of AIDS-related lymphomas. Immunoblastic B-cell lymphomas frequently arise in immune-compromized individuals such as allograft recipients and others receiving long-term immunosuppressive therapy, AIDS patients, and patients with primary immunodeficiency syndromes such as X-linked lymphoproliferative syndrome or Wiscott-Aldrich syndrome (Thomas et al., Adv. Cancer Res. 57, 329 (1991); Straus et al., Ann. Intern. Med. 118, 45 (1993). Malignant B cells from several tumors of B-cell lineage express a high degree of CD40 and appear to depend on CD40 signaling for survival and proliferation.


CD40-CD40L interactions may also promote immune-mediated angiogenesis, gut inflammation, acute intestinal injury or chronic intestinal injury, in the pathogenesis of inflammatory bowel disease (IBD). For example, the engagement of CD40L-activated HIF supernatants induce angiogenic events as determined by migration of HUMECs and tubule formation, both of which are inhibited using antibodies that bind to vascular endothelial growth factor (VEGF), interleukin-8 (IL-8) or hepatocyte growth factor (HGF). Additionally, CD40-deficient and CD40L-deficient mice are protected from DSS-induced colitis and display significant impairment of gut inflammation-driven angiogenesis, as determined by their microvascular density (Danese et al., Gut 56, 1248-1256, 2007).


CD40-CD40L interaction also activates extracellular signal-regulated kinase 1/2 and nuclear factor-κB pathways in insulinoma NIT-1 cells, and inhibitors of either pathway suppress cytokine/chemokine production in islets and up-regulate intercellular adhesion molecule-1 associated with inflammation, contributing to early islet graft loss after transplantation (Barbé-Tuana et al., Diabetes 55, 2437-2445, 2006).


Cell types typically resident in atherosclerotic plaques e.g., endothelial cells, macrophages and smooth muscle cells also express CD40L, and exposure to CD40L stimulates a broad inflammatory response in these cells such as heightened expression of pro-inflammatory cytokines, adhesion molecules, matrix degrading enzymes, and pro-coagulants, thereby leading to atherogenesis and lesion complication (Alderson et al., J Exp Med. 178, 669-674, 1993; Mach et al., Proc. Natl. Acad. Sci. USA 94, 1931-1936, 1997; Schonbeck et al., Circ Res. 89, 1092-1103, 2001; Mach et al., Nature 394, 200-203, 1998; Bavendiek et al., Arterioscler Thromb Vase Biol. 25, 1244-1249, 2005). Animals that are deficient in CD40L have reduced levels of atherosclerosis on high-cholesterol diets and atherosclerotic lesions in such animals display features associated with plaque stability e.g., reduced macrophage count, reduced lipid content, increased collagen content (Lutgens et al., Nat. Med. 5, 1313-1316, 1999; Schonbeck et al., Proc. Natl. Acad. Sci. USA 97, 7458-7463, 2000). The soluble 18 kDa CD40L protein released from platelets on platelet activation may identify first or recurrent cardiovascular events, which further supports the pathogenic role of CD40L (Heeschen et al., N. Engl. J. Med. 348, 1104-1111, 2003; Schonbeck et al., Circulation 104, 2266-2268, 2001; Varo et al., Circulation 107, 2664-2669, 2003). Recently, Zirlik et al., Circulation 115, 1571-1580, 2007 demonstrated that CD40L interacts with Mac-1 on monocytes, and functionally enhances Mac-1 dependent monocyte adhesion and migration in vitro, and that inhibition of Mac-1 in vivo in LDLR−/− mice slows lesion development and macrophage accumulation in atherosclerotic plaques. Zirlik et al. suggest that the CD40L-Mac-1 interaction may participate, albeit not necessarily exclusively, in the expression of several pro-inflammatory cytokines including MIP-2, interleukin-1β, IL-8, pro-coagulant tissue factor, and in the activation of pro-inflammatory NF-κB. Thus, CD40L not only may attract inflammatory cells via Mac-1, but also induces the expression of a variety of pro-inflammatory and pro-oxidant functions that promote atherogenesis.


Elevated soluble CD40L is also prognostic of an increased risk of thrombosis and cardiac ischemia.


Modulators of Protein-Protein Interactions

To identify suitable therapeutic compounds, the pharmaceutical industry has particularly focussed on screening processes to identify antibodies, peptides and small molecule compounds capable of interacting with a protein and/or inhibiting a protein interaction. To function as a drug suitable for administration to a subject an antibody, peptide or small molecule must be capable of binding to a target with high affinity and selectivity.


Peptides offer significant advantages over antibodies in terms of uptake and low immunogenicity, and over small molecules in terms of reduced toxicity.


1. Molecular Shape Considerations


Often, small molecules and short peptides do not effectively modulate protein interactions because they do not generally possess a required shape e.g., to fit into complex protein surfaces or bind to relatively featureless interfaces. As a consequence, small-molecules ands short peptides are generally unable to bind to many surfaces of a target protein with sufficiently-high affinity and specificity to modulate binding of a ligand to the target, or to otherwise agonize or antagonize the activity of the target protein. Accordingly, there is a high attrition rate for the screening of such molecules as drug leads for therapeutic applications, particularly for targets such as protein interactions.


2. Random Peptides


By way of example, notwithstanding that short random peptides e.g., peptide aptamers, may be sufficiently small for commercial i.e., large-scale production by chemical synthesis, they generally provide highly-variable bioactivities against target proteins, and interactions with their targets are generally low affinity interactions. For example, in a screen of a random peptide library to identify a peptide capable of dissociating HIV protease fewer than about 1×10-6 peptides displayed the desired activity (Park and Raines Nat. Biotechnol., 18: 548-550, 2000). This low “hit” rate appears to be a result of the inability of the such random peptides to assume stable secondary structure and/or tertiary structure to thereby facilitate binding to a target protein.


3. Structural Constraint


In response to the low “hit” rate for identifying new drug leads, the pharmaceutical industry has expended some effort in developing synthetic scaffolds for presenting ligands to proteins, with a view to modulating activity of the target protein. However, such constraint of random peptide libraries has failed to increase the “hit” rate for identifying new drug candidates based on random peptide sequences to a level that makes peptides a viable alternative to small molecules. For example, random peptides have been constrained within scaffold structures e.g., the active site loop of thioredoxin (“Trx”; Colas et al., Nature, 380: 548-550, 1996) and tested for binding to cyclin-dependent kinase-2 (Cdk-2), however fewer than 2×10-5 of the Trx-constrained peptides actually blocked the target. Thus, the provision of synthetic scaffolds does not necessarily enhance “hit” rate. It is also possible that the limited repertoire of artificial scaffolds available to the industry will necessarily limit the diversity of structures that can be produced using such approaches, and may even mask or modify any native structures formed.


4. Secondary Structures, Domains, Sub-Domains and Folds


Native proteins have considerable structural features, including protein “domains” that are generally of functional significance. Until the present invention, such structural features have largely been utilized to determine evolutionary relationships between proteins, and for dissecting dynamic folding pathways i.e., how particular proteins fold. For example, the CATH database (Orengo et al., Structure 5, 1093-1108, 1997) classifies proteins according to a hierarchy of Class, Architecture, Topology and Homologous superfamily based upon structure, sequence, and functional considerations. In particular, the CATH hierarchy acknowledges three basic structural features i.e., class, architecture and topology. Protein “class” is highest in the CATH hierarchy and, in this context is a reference to the secondary structure composition and packing of a protein i.e., mainly α-helix, mainly β-strand, and α−β including alternating α/β in which the secondary structures alternate along the protein chain, and α+β in which the α and β regions are largely segregated. Thus, the “class” to which a protein belongs is a global assignment based on secondary structure considerations. Protein “architecture” refers to the overall shape of a protein based upon groups of similar secondary structural arrangements irrespective of the order in which they are connected in the protein. Protein “topology” describes the relative associations and orientations of secondary structures in 3D and the order in which they are connected. Protein “folds” are recognized in the CATH hierarchy as a function of topology, however the literature is confusing in this respect, because a fold can adopt a specific architecture e.g., Orengo and Thornton, Ann. Rev. Biochem. 74, 867-900, 2005.


As used herein, the term “fold” is therefore taken in its broadest context to mean a tertiary structure formed by the folding of multiple secondary structures including aspects of both architecture and topology. Herein, the term “subdomain” is used interchangeably with the term “fold”. A “fold” may form independently or in association with other parts of a protein or other proteins or a scaffold structure.


Table 1 herein includes descriptions of segments of proteins comprising protein domains.









TABLE 1







Exemplary structures adopted by homologous superfamilies of proteins








Structure
Architecture and/or topology of folds within proteins





α-helix
α-helices; folded leaf, partly opened


α-helix
2α-helices; antiparallel hairpin, left-handed twist


α-helix
tandem repeat of two calcium-binding loop-helix motifs comprising α--helices


α-helix
helix-extended loop-helix; parallel α-helices


α-helix
2α-helices: one short, one long; aromatic-rich interface


α-helix
3α-helices; folded leaf, opened


α-helix
3-α-helices; bundle, closed or partly opened, right-handed twist; up-and down


α-helix
3-α-helices; bundle, closed or partly opened, right-handed twist; up-and down


α-helix
3α-helices; bundle, right-handed twist


α-helix
3-4α-helices


α-helix
3α-helices; architecture is similar to that of the “winged helix” fold


α-helix
3α-helices; bundle, closed, left-handed twist; up-and-down


α-helix
3α-helices; bundle, closed, left-handed twist; up-and-down; mirror topology to



the spectrin-like fold


α-helix
3α-helices; bundle, closed, right-handed twist; up-and-down


α-helix
3α-helices; bundle, closed, left-handed twist, up-and-down


α-helix
core: 3α-helices; bundle, closed, left-handed twist; up-and-down


α-helix
3α-helices; bundle, partly opened


α-helix
3α-helices, the first one is shorter than the other two; bundle, partly opened


α-helix
3 short α-helices; irregular array


α-helix
3 short α-helices; irregular array


α-helix
3α-helices; irregular array


α-helix
3α-helices; irregular array; disulfide-rich


α-helix
α-helices; irregular array; disulfide-rich


α-helix
3α-helices; irregular array


α-helix
3α-helices; bundle, closed, right-handed twist; up-and-down


α-helix
3α-helices; bundle, closed, left-handed twist; parallel


α-helix
3α-helices; irregular array


α-helix
3α-helices; long middle helix is flanked at each end with shorter ones


α-helix
3α-helices; bundle, open


α-helix
α-helices; irregular array


α-helix
4α-helices; bundle, closed or partly opened, left-handed twist; up-and-down


α-helix
4α-helices; bundle, closed, right-handed twist; 1 crossover connection


α-helix
4α-helices; bundle, closed, left-handed twist; 1 crossover connection


α-helix
4α-helices; bundle, closed; left-handed twist; 2 crossover connections


α-helix
4α-helices; bundle; one loop crosses over one side of the bundle


α-helix
4α-helices, bundle; helix 3 is shorter than others; up-and-down


α-helix
4α-helices; bundle; minor mirror variant of up-and-down topology


α-helix
4α-helices; dimer of identical alpha-hairpin subunits; bundle, closed, left-handed



twist


α-helix
4α-helices; bundle, closed, right-handed twist


α-helix
4α-helices; bundle, closed, right-handed twist


α-helix
4α-helices; bundle, closed, right-handed twist


α-helix
4α-helices; bundle, closed, left-handed twist


α-helix
4α-helices; bundle, closed, right-handed twist


α-helix
4α-helices; folded leaf, closed


α-helix
4α-helices; orthogonal array


α-helix
4α-helices; the long C-terminal helix protrudes from the domain and binds to



DNA


α-helix
4-α-helices; bundle, closed, left-handed twist; 2 crossover connections


α-helix
4α-helices; array of 2 hairpins, opened


α-helix
4α-helices: bundle


α-helix
4α-helices: bundle


α-helix
4α-helices: open bundle; capped by two small 3-stranded beta-sheets



duplication: consists of two structural repeats


α-helix
4α-helices: bundle; flanked by two short beta-hairpins duplication: consists of



two structural repeats


α-helix
4α-helices; array of 2 hairpins, opened


α-helix
4 helices; bundle, closed, left-handed twist; right-handed super helix


α-helix
4α-helices; bundle, left-handed twist; right-handed super helix


α-helix
4α-helices; bundle, right-handed twist; right-handed super helix


α-helix
4 long α-helices; bundle, left-handed twist (coiled coil); right-handed super helix


α-helix
4α-helices; bundle, left-handed twist; left-handed super helix


α-helix
4α-helices; bundle, right-handed twist; left-handed super helix


α-helix
4α-helices; irregular array


α-helix
2α-helices and adjacent loops


α-helix
4α-helices; irregular array


α-helix
4α-helices; irregular array


α-helix
4α-helices; irregular array, disulfide-linked


α-helix
4α-helices irregular array, disulfide-linked


α-helix
4α-helices; irregular array, disulfide-linked


α-helix
4α-helices; folded leaf; right-handed super helix


α-helix
4α-helices; folded leaf; right-handed super helix


α-helix
4α-helices; bundle


α-helix
4 long α-helices; bundle


α-helix
4 helices; bundle, partly opened


α-helix
core: 4α-helices; bundle, partly opened, capped with a beta-sheet


α-helix
4α-helices, bundle


α-helix
4 helices; the three last helices form a bundle similar to that of the RuvA C-



domain


α-helix
4α-helices; an orthogonal array


α-helix
4α-helices; an orthogonal array


α-helix
4α-helices; up-and-down bundle


α-helix
4α-helices; open up-and-down bundle; binds alpha-helical peptides


α-helix
4α-helices; open up-and-down bundle; flexible N-terminal tail


α-helix
4α-helices; array


α-helix
4α-helices; bundle, closed, left-handed twist


α-helix
4α-helices dimer of identical alpha-hairpin subunits; open bundle


α-helix
4-5α-helices; bundle of two orthogonally packed alpha-hairpins


α-helix
4-5α-helices; right-handed super helix


α-helix
5α-helices; right-handed super helix; swapped dimer with the two long C-



terminal helices


α-helix
α-helices array; two long helices form a hairpin that dimerizes into a 4-helical



bundle


α-helix
5α-helices; bundle, closed, left-handed twist


α-helix
5α-helices; bundle, closed, left-handed twist


α-helix
5α-helices; bundle, closed, left-handed twist; helices 2-5 adopt the Four-helical



up-and-down bundle fold


α-helix
5α-helices; bundle, closed, left-handed twist


α-helix
5α-helices; folded leaf, closed


α-helix
5α-helices; folded leaf, closed


α-helix
5α-helices; folded leaf


α-helix
5α-helices; irregular array; left-handed super helix


α-helix
4-5α-helices; bundle; left-handed super helix


α-helix
5α-helices; bundle


α-helix
5α-helices; bundle


α-helix
α-helices; bundle


α-helix
5α-helices; bundle


α-helix
α-helices; one helix is surrounded by the others


α-helix
5α-helices; one helix is surrounded by the others


α-helix
5α-helices; one helix is surrounded by the others


α-helix
5α-helices; contains one more helix and a beta-hairpin outside the core


α-helix
5α-helices: bundle


α-helix
α-helical bundle; up-and-down; right-handed twist


α-helix
5α-helices: orthogonal array


α-helix
5α-helices: orthogonal array


α-helix
5α-helices: irregular array


α-helix
5α-helices; array


α-helix
5α-helices; orthogonal array; folding similarity to the TipA-S domain


α-helix
5α-helices; array


α-helix
6α-helices: bundle; left-handed twist, up-and-down topology


α-helix
6α-helices, homodimer of 3-helical domains


α-helix
6α-helices, homodimer of 3-helical domains


α-helix
6α-helices, homodimer of 3-helical domains


α-helix
6α-helices, heterodimer of 3-helical domains


α-helix
dimer of 3α-helical segments; consists of two subdomains: 4-helical bundle and



coiled coil


α-helix
6α-helices: closed bundle; greek-key; internal pseudo twofold symmetry


α-helix
6α-helices: closed bundle; greek-key; internal pseudo twofold symmetry


α-helix
6α-helices: bundle; one central helix is surrounded by 5 others


α-helix
6α-helices; bundle; one central helix is surrounded by 5 others


α-helix
6α-helices: array


α-helix
6α-helices: orthogonal array


α-helix
irregular array of 6 short α-helices


α-helix
6α-helices; one central helix is surrounded by 5 others


α-helix
6α-helices; one central helix is surrounded by 5 others


α-helix
6α-helices; bundle; one central helix is surrounded by 5 others


α-helix
Multiple α-helices


α-helix
Multihelical; core: 5-helical bundle


α-helix
multihelical; contains compact array of 6 short helices


α-helix
multihelical; irregular array of long and short helices


α-helix
multihelical; irregular array of long and short helices


α-helix
multihelical bundle; contains buried central helix


α-helix
multihelical; contains two buried central helices


α-helix
multihelical; can be divided into two subdomains


α-helix
multihelical; consists of two all-alpha subdomains



contains a 4-helical bundle with left-handed twist and up-and-down topology


α-helix
multihelical; consists of two all-alpha subdomains each containing a 3-helical



bundle with right-handed twist


α-helix
multihelical; consists of two all-alpha subdomains; contains a 4-helical bundle



with left-handed twist and up-and-down topology


α-helix
multihelical; consists of two tightly associated 3-helical bundles with different



twists


α-helix
multihelical; consists of two all-alpha subdomains; dimer


α-helix
multihelical; consists of two all-alpha subdomains


A-helix
multihelical; consists of two all-alpha domains


A-helix
multihelical; consists of two different 3-helical domains connected by a long,



partly helical linker


α-helix
multihelical; consists of two different alpha-helical bundles (4-helical and 3-



helical)


α-helix
multihelical; consists of two different alpha-helical bundles


α-helix
multihelical; consists of two different alpha-helical bundles


α-helix
multihelical; consists of two different all-alpha subdomains, 4 helices each


α-helix
multihelical; consists of two all-alpha domains


α-helix
multihelical; consists of two all-alpha domains


α-helix
multihelical; consists of two all-alpha subdomains


α-helix
multihelical consists of two all-alpha subdomains



subdomain 1 (residues 10-100) is a 4-helical bundle


α-helix
multihelical


α-helix
multihelical; consists of two all-alpha subdomains


α-helix
multihelical; common core is formed around two long antiparallel helices related



by (pseudo) twofold symmetry


α-helix
multihelical


α-helix
multihelical; up to seven alpha-hairpins are arranged in closed circular array


α-helix
multihelical; consists of two all-alpha domains


α-helix
multihelical


α-helix
multihelical; forms intertwined dimer of identical 5-helical subunits


α-helix
multihelical; intertwined tetramer


α-helix
multihelical; intertwined trimer of identical 3-helical subunits


α-helix
multihelical; consists of two all-alpha domains


α-helix
multihelical; core: 5-helical bundle; binds cofactor at the beginning of third helix


α-helix
multihelical; contains a 3-helical bundle surrounded by several shorter helices


α-helix
multihelical; contains a 3-helical Hin recombinase-like subdomain and two long



dimerisation helices


α-helix
multihelical oligomeric protein


α-helix
multihelical; consists of a conserved 4-helical core and a variable insert



subdomain


α-helix
multihelical; consists of 2 all-alpha subdomains


α-helix
multihelical; consists of 2 all-alpha subdomains, “rigid” one and “mobile” one


α-helix
multihelical; consists of 2 all-alpha subdomains connected by a long helix


α-helix
multihelical; array of longer and shorter helices; contains an alpha-hairpin



dimerisation subdomain


α-helix
multihelical; bundle of longer and shorter helices


α-helix
multihelical; three-helical bundle in the core is surrounded by non-conserved



helices


α-helix
multihelical; consists of two subdomains


α-helix
multihelical


α-helix
multihelical


α-helix
multihelical; can be divided into an alpha-alpha super helix domain and a long



alpha-hairpin dimerization domain


α-helix
multihelical; can be divided into three subdomains (neck, body and tail)


α-helix
multihelical; 2 (curved) layers: alpha/alpha; right-handed super helix


α-helix
multihelical


α-helix
multihelical; consists of two all-alpha subdomains


α-helix
multihelical; interlocked (homo)dimer


α-helix
multihelical; interlocked heterodimer with F-box proteins


α-helix
multihelical; interlocked heterodimer with the Skp1 dimerisation domain


α-helix
multihelical; 3 layers or orthogonally packed helices


α-helix
multihelical


α-helix
multihelical; consist of two subdomains


α-helix
multihelical; open array


α-helix
multihelical; 2 layers or orthogonally packed helices


α-helix
multihelical bundle; contains buried central helix


α-helix
multihelical; consists of two topologically similar alpha-helical bundles


α-helix
multihelical; consists of 2 four-helical bundles


α-helix
multihelical; one domain consists of two similar disulfide-linked subdomains


α-helix
multihelical, consists of three all-alpha domains


α-helix
multihelical, consists of three all-alpha domains


α-helix
multihelical; core: 8 helices (C-J) are arranged in 2 parallel layers


α-helix
multihelical; 8 helices arranged in 2 parallel layers


α-helix
multihelical; bundle


α-helix
multihelical; core: 6 helices, bundle


α-helix
multihelical; forms a boat-shaped protein shell around cofactors


α-helix
multihelical; bundle


α-helix
multihelical; contains 4-helical bundle and 2-helical arm


α-helix
multihelical; array


α-helix
multihelical; array


α-helix
multihelical; bundle


α-helix
multihelical; bundle


α-helix
multihelical; bundle


α-helix
multihelical; array


α-helix
common core: 2 helices, disulfide-linked, and a calcium-binding loop


α-helix
5 helices: irregular disulfide-linked array; also contains a small beta-hairpin


α-helix
5 helices: irregular disulfide-linked array; form homodimer


α-helix
5 helices: irregular disulfide-linked array; topological similarity to the Fungal



elicitin fold


α-helix
6 helices: irregular non-globular array; also contains two small b-hairpins


α-helix
3 helices, non-globular array; forms interlocked heterodimers with its targets


α-helix
variable number of helices and little beta structure


β-sheet
sandwich; 7 strands in 2 sheets; greek-key


β-sheet
sandwich; 9 strands in 2 sheet; greek-key; subclass of immunoglobin-like fold


β-sheet
sandwich; 7 strands in 2 sheets, greek-key


β-sheet
sandwich; 6 strands in 2 sheets


β-sheet
sandwich; 6 strands in 2 sheets


β-sheet
sandwich; 6 strands in 2 sheets


β-sheet
six-stranded beta-sandwich, jelly-roll/greek-key topology


β-sheet
sandwich; 7 strands in 2 sheets, greek-key


β-sheet
sandwich; 7 strands in 2 sheets, greek-key; permutation of the immunoglobulin-



like fold


β-sheet
sandwich; 8 strands in 2 sheets; greek-key


β-sheet
sandwich; 8 strands in 2 sheets; greek-key


β-sheet
sandwich; 8 strands in 2 sheets; meander


β-sheet
sandwich; 8 strands in 2 sheets; meander


β-sheet
sandwich; 8 strands in 2 sheets; jelly-roll; some members can have additional 1-2



strands


β-sheet
sandwich; 8 strands in 2 sheets; greek-key


β-sheet
sandwich; 8 strands in 2 sheets; complex topology


β-sheet
sandwich; 8 strands in 2 sheets; jelly-roll


β-sheet
sandwich; 8 strands in 2 sheets; jelly-roll; similarity to the Nucleoplasmin-like/VP



fold


β-sheet
sandwich; 8 strands in 2 sheets; jelly-roll


β-sheet
sandwich; 8 strands in 2 sheets; jelly-roll


β-sheet
sandwich; 8 strands in 2 sheets; greek-key


β-sheet
beta-sandwich: 8 strands in 2 sheets


β-sheet
sandwich; 8 strands in 2 sheets; complex topology with the crossing loops


β-sheet
sandwich; 8 strands in 2 sheets; greek-key: partial topological similarity to



immunoglobulin-like folds


β-sheet
sandwich; 8 strands in 2 sheets; greek-key: partial topological similarity to



immunoglobulin-like folds


β-sheet
sandwich; 8 strands in 2 sheets; greek-key: partial topological similarity to



immunoglobulin-like folds


β-sheet
sandwich; 9 strands in 2 sheets; jelly-roll


β-sheet
sandwich; 9 strands in 2 sheets; jelly-roll; form trimers


β-sheet
sandwich; 9 strands in 2 sheets; greek-key


β-sheet
sandwich; 9 strands in 2 sheets; greek-key


β-sheet
sandwich; 9 strands in 2 sheets; greek-key/jelly-roll


β-sheet
sandwich; 9 strands in 2 sheets; jelly-roll


β-sheet
sandwich; 9 strands in 2 sheets; greek-key; contains a few helices in loop regions


β-sheet
sandwich; 9 strands in 2 sheets; unusual topology with 2 crossover loops


β-sheet
sandwich, 10 strands in 2 sheets; greek-key


β-sheet
sandwich, 10 strands in 2 sheets; jelly-roll


β-sheet
sandwich, 10 strands in 2 sheets; jelly-roll


β-sheet
sandwich, 10 strands in 2 sheets; “folded meander”


β-sheet
sandwich, 10 strands in 2 sheets


β-sheet
sandwich; 11 strands in 2 sheets


β-sheet
sandwich; 11 strands in 2 sheets; greek-key


β-sheet
sandwich; 11 strands in 2 sheets; greek-key


β-sheet
sandwich; 14 strands in 2 sheets; greek-key


β-sheet
sandwich; 12-14 strands in 2 sheets; complex topology


β-sheet
sandwich; 18 strands in 2 sheets


β-sheet
duplication: two beta-sandwiches of similar topologies are fused together in a



single three beta-sheet domain


β-sheet
consists of two beta-sandwich domains of similar topologies


β-sheet
consists of two different beta-sandwich domains of partial topological similarity



to immunoglobulin-like folds


β-sheet
consists of two different beta-sandwich domains unrelated to other beta-sandwich



folds


β-sheet
consists of two all-beta subdomains: conserved small domain has a rubredoxin-



like fold; larger domain consists of 6 beta-stands packed in either sandwich of



two 3-stranded sheets or closed barrel (n = 6; S = 8)


β-sheet
this fold is formed by three glycine-rich regions inserted into a small 8-stranded



beta-sandwich


β-sheet
barrel, partly opened; n* = 4, S* = 8; meander


β-sheet
contains barrel, partly opened; n* = 4, S* = 8; meander


β-sheet
contains barrel, partly opened; n* = 4, S* = 8; meander; capped by alpha-helix


β-sheet
core: barrel, in some members open; n* = 4, S* = 8; meander


β-sheet
core: barrel, open; n* = 4, S* = 8; meander; SH3-like topology


β-sheet
core: barrel, open; n* = 4, S* = 8; meander; SH3-like topology; some similarity to



the Sm-like fold


β-sheet
core: barrel, open; n* = 4, S* = 8; meander; SH3-like topology; some similarity to



the Sm-like fold


β-sheet
core: barrel, closed; n = 4, S = 8; complex topology; helix-containing crossover



connection


β-sheet
barrel, closed; n = 5, S = 8, meander


β-sheet
barrel, closed or partly opened n = 5, S = 10 or S = 8; greek-key


β-sheet
core: barrel, partly opened; n* = 5, S* = 8; meander


β-sheet
barrel, closed; n = 6, S = 12; and a hairpin triplet; meander


β-sheet
barrel, closed; n = 6, S = 10; greek-key


β-sheet
barrel, closed; n = 6, S = 10; greek-key


β-sheet
barrel; n = 6, S = 10; greek-key


β-sheet
core: barrel; n = 6, S = 10; greek-key; topologically similar to the FMN-binding split



barrel


β-sheet
segment-swapped dimer forming two identical conjoint barrels (n = 6, S = 10)



topologically similar to the FMN-binding split barrel


β-sheet
barrel, open; n* = 6, S* = 10; greek-key


β-sheet
barrel, closed; n = 6, S = 8; greek-key


β-sheet
barrel; n = 6, S = 8, greek-key; similar to one trypsin-like protease barrel


β-sheet
barrel; n = 6, S = 8, greek-key


β-sheet
barrel, closed; n = 6, S = 8; greek-key


β-sheet
barrel, closed; n = 6, S = 8, greek-key, partial similarity to the OB-fold


β-sheet
barrel, closed; n = 6, S = 10, complex topology


β-sheet
core: barrel, closed; n = 6, S = 8; topology is similar to that of the acid proteases



barrel


β-sheet
barrel, closed; n = 6, S = 8; a crossover loop topology


β-sheet
barrel, closed; n = 6, S = 10; complex topology with crossover (psi) loops


β-sheet
barrel, closed; n = 6, S = 10; complex topology


β-sheet
barrel, closed; n = 6, S = 10; meander; capped at both ends by alpha-helices


β-sheet
barrel, partly opened; n* = 6, S* = 12; meander; capped by an alpha-helix


β-sheet
barrel, closed; n = 6, S = 12; mixed beta-sheet


β-sheet
core: barrel, closed; n = 7, S = 8; complex topology


β-sheet
barrel, closed; n = 7, S = 10; complex topology


β-sheet
barrel, closed; n = 7, S = 10; order: 1234765; strands 1 and 5 are parallel to each



other


β-sheet
barrel, closed; n = 7, S = 10; complex topology


β-sheet
barrel, closed; n = 7, S = 10; greek-key topology; one overside connection


β-sheet
barrel, closed; n = 7, S = 10; complex topology


β-sheet
core: barrel, closed; n = 7, S = 12; meander


β-sheet
barrel, closed or opened; n = 8, S = 12; meander


β-sheet
barrel, closed; n = 8, S = 10; meander


β-sheet
barrel, closed; n = 8, S = 10; complex topology


β-sheet
barrel, closed; n = 8, S = 10; one overside connection


β-sheet
barrel, closed; n = 8, S = 10; mixed sheet; two overside connections


β-sheet
barrel, partly open; n* = 8, S* = 10; one psi loop


β-sheet
dimer of two non-identical subunits; forms two similar barrels, n = 8, S = 10 each,



that are fused together with the formation of third barrel, n = 6, S = 8


β-sheet
consists of four 4-stranded beta-sheet motifs; meander


β-sheet
consists of five 4-stranded beta-sheet motifs; meander


β-sheet
consists of six 4-stranded beta-sheet motifs; meander


β-sheet
consists of seven 4-stranded beta-sheet motifs; meander


β-sheet
consists of eight 4-stranded beta-sheet motifs; meander


β-sheet
folded sheet; greek-key


β-sheet
core: 3-stranded meander beta-sheet


β-sheet
small mixed beta-sheet, 4 “generalized” strands


β-sheet
coiled antiparallel beta-sheet of 5 strands, order 51324; complex topology,



crossing loops


β-sheet
twisted meander beta-sheet of 6 strands


β-sheet
core: twisted 7-stranded beta-sheet (half-barrel) of complex topology


β-sheet
core: twisted 7-stranded beta-sheet (half-barrel)


β-sheet
single sheet; 10 strands


β-sheet
11 stranded sheet partly folded in a corner-like structure filled with a few short



helices


β-sheet
single sheet; 16 strands; meander


β-sheet
single sheet formed by beta-hairpin repeats; exposed on both sides in the middle


β-sheet
consists of 3 4-stranded sheets; strands are parallel to the 3-fold axis


β-sheet
consists of 3 4-stranded sheets; strands are perpendicular to the 3-fold axis


β-sheet
superhelix turns are made of parallel beta-strands and (short) turns


β-sheet
superhelix turns are made of parallel beta-strands and (short) turns


β-sheet
one turn of helix is made by two pairs of antiparallel strands linked with short



turns


β-sheet
(homo)trimer; each chain donates 3 beta-strands per turn of the helix


β-sheet
trimer formed by the interlocking beta-hairpin repeat units


β-sheet
trimer; contains two different beta-prism-like domains connected by an linker



subdomain of less regular structure


β-sheet
Trp-rich beta-hairpin repeat units form helical structures of 3 units per turn


β-sheet
sandwich of half-barrel shaped beta-sheets


β-sheet
double-stranded ribbon sharply bent in two places; the ribbon ends form



incomplete barrel; jelly-roll


β-sheet
multisheet protein with a mixture of beta-sandwich and beta-prism features


β-sheet
multisheet protein containing partial beta-propeller and beta-sandwich regions


β-sheet
multisheet protein with a mixture of beta-sandwich and beta-barrel features


β-sheet
complex fold made of five beta-hairpin units and a b-ribbon arc


β-sheet
complex fold made of several coiled beta-sheets; contains an SH3-like barrel


β-sheet
complex fold made of several coiled beta-sheets


β-sheet
complex fold made of several coiled beta-sheets


β-sheet
complex fold


β-sheet
complex fold; consists of two intertwined subdomains


β-sheet
complex fold


β-sheet
complex fold made of bifurcated and partly folded beta-sheet


β-sheet
complex fold made of bifurcated and coiled beta-sheets


β-sheet
complex fold made of bifurcated and coiled b-sheets


β-sheet
pseudobarrel; mixed sheet of 7 strand folded upon itself and “buckled” by two



beta-turns


β-sheet
pseudobarrel; sandwich of two sheets packed at a positive interstrand angle and



interconnected with many short turns


β-sheet
pseudobarrel; capped on both ends by alpha-helices


β-sheet
pseudobarrel; capped at one end by an alpha-helix


β-sheet
pseudobarrel; capped on both ends by alpha-helices


β-sheet
pseudobarrel; mixed folded sheet of 5 strands; order 13452; strand 1 and 3 are



parallel to each other


β-sheet
pseudobarrel; some similarity to OB-fold


β-sheet
non-globular proline-rich hairpin


α/β
contains parallel beta-sheet barrel, closed; n = 8, S = 8; strand order 12345678


α/β
core: 3 layers, a/b/a; parallel beta-sheet of 6 strands, order 321456


α/β
core: 3 layers, b/b/a; central parallel beta-sheet of 5 strands, order 32145; top



antiparallel beta-sheet of 3 strands, meander


α/β
3 layers: a/b/a; parallel beta-sheet of 5 strands, order 32145; Rossmann-like


α/β
3 layers: a/b/a; parallel beta-sheet of 5 strands, order 32145; incomplete



Rossmann-like fold; binds UDP group


α/β
variant of beta/alpha barrel; parallel beta-sheet barrel, closed, n = 7, S = 8; strand



order 1234567; some members may have fewer strands


α/β
contains: barrel, closed; n = 10, S = 10; accommodates a hairpin loop inside the



barrel


α/β
3 layers: b/b/a; the central sheet is parallel, and the other one is antiparallel; there



are some variations in topology


α/β
2 layers, a/b; parallel beta-sheet of 3 strands, order 123


α/β
core: 3 layers, a/b/a; parallel beta-sheet of 4 strands, order 1234; structural



similarity of the MurF and HprK extends beyond the core.


α/β
2 curved layers, a/b; parallel beta-sheet; order 1234...N; there are sequence



similarities between different superfamilies


α/β
core: three turns of irregular (beta-beta-alpha)n superhelix


α/β
core: 4 turns of a (beta-alpha)n superhelix


α/β
core: 4 turns of (beta-beta-alpha)n superhelix


α/β
3 layers, a/b/a; core: parallel beta-sheet of 4 strands, order 2134


α/β
3 layers, a/b/a; core: parallel beta-sheet of 4 strands, order 2134


α/β
3 layers, a/b/a; core: parallel beta-sheet of 4 strands, order 2134


α/β
3 layers, a/b/a; core: parallel beta-sheet of 4 strands, order 2134


α/β
3 layers, a/b/a; parallel beta-sheet of 4 strands, order 2134


α/β
3 layers, a/b/a; core: parallel beta-sheet of 4 strands, order 2134


α/β
core: 3 layers: a/b/a; parallel beta-sheet of 4 strands; 2134


α/β
3 layers, a/b/a; parallel beta-sheet of 4 strands, order 2134


α/β
3 layers, a/b/a; parallel beta-sheet of 4 strands, order 2134


α/β
3 layers, a/b/a; core: parallel beta-sheet of 4 strands, order 3214


α/β
3 layers, a/b/a; core: parallel beta-sheet of 4 strands, order 1423


α/β
3 layers, a/b/a; parallel beta-sheet of 5 strand, order 21345


α/β
3 layers, a/b/a; parallel beta-sheet of 5 strands, order 32145


α/β
3 layers, a/b/a; parallel beta-sheet of 5 strands, order 32145


α/β
core: 3 layers, a/b/a; parallel beta-sheet of 5 strands, order 32145


α/β
3 layers: a/b/a; parallel beta-sheet of 5 strands, order 32145; Rossmann-like


α/β
3 layers: a/b/a; parallel beta-sheet of 5 strands, order 32145; Rossmann-like


α/β
3 layers: a/b/a, core: parallel beta-sheet of 5 strands, order 43215


α/β
3 layers, a/b/a; core: parallel beta-sheet of 5 strands, order 32145


α/β
3 layers: a/b/a, core: parallel beta-sheet of 5 strands, order 21354; topological



similarity to a part of the arginase/deacetylase fold


α/β
core: 3 layers: a/b/a, parallel beta-sheet of 5 strands, order 21435; contains a deep



trefoil knot


α/β
3 layers: a/b/a; parallel or mixed beta-sheet of 4 to 6 strands


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 321456; Rossmann-like


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 321456


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 321456


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 321456; also contains a C-



terminal alpha + beta subdomain


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 321456


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 321456


α/β
core: 3 layers: a/b/a; parallel or mixed beta-sheet of 6 strands, order 321456


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 321456


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 432156


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 342156


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 213456


α/β
3 layers: a/b/a; parallel beta-sheet of 6 strands, order 213465


α/β
3 layers: a/b/a, parallel or mixed beta-sheets of variable sizes


α/β
3 layers: a/b/a, parallel beta-sheet of 6 strands, order 324156


α/β
3 layers, a/b/a; parallel beta-sheet of 7 strands, order 7165243


α/β
3 layers: a/b/a, parallel beta-sheet of 7 strands, order 3214567


α/β
3 layers: a/b/a, parallel beta-sheet of 7 strands, order 4321567


α/β
3 layers: a/b/a, parallel beta-sheet of 7 strands, order 3421567


α/β
3 layers: a/b/a, parallel beta-sheet of 7 strands, order 2314567; left-handed



crossover connection between strands 2 & 3


α/β
core: 3 layers, a/b/a; parallel beta-sheet of 7 strands, order 2134756


α/β
3 layers: a/b/a, parallel beta-sheet of 8 strands, order 21387456


α/β
3 layers: a/b/a; parallel beta-sheet of 8 strands, order 54321678


α/β
beta(2)-(alpha-beta)2-beta; 2 layers, a/b; mixed beta-sheet of 5 strands, order



12345; strands 1 & 5 are antiparallel to the rest


α/β
beta(2)-(alpha-beta)2-beta(3); 3 layers, a/b/b; some topological similarity to the



N-terminal domain of MinC


α/β
core: 2 layers, a/b; mixed beta-sheet of 6 strands, order 324561; strands 3 & 6 are



antiparallel to the rest


α/β
3 layers: a/b/a; parallel beta-sheet of 4 strands, order 2134


α/β
core: 3 layers, a/b/a; parallel beta-sheet of 4 strands, order 1423


α/β
3 layers: a/b/a; parallel beta-sheet of 5 strands, order 32451


α/β
core: 3 layers, a/b/a; mixed beta-sheet of 4 strands, order 4312; strand 3 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 4 strands, order 2143, strand 4 is antiparallel



to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 5 strands, order 13245, strand 1 is antiparallel



to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 5 strands, order 32145, strand 5 is antiparallel



to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of five strands, order 21345; strand 4 is



antiparallel to the rest


α/β
core: 3 layers, b + a/b/a; the central mixed sheet of 5 strands: order 21534; strand



2 is antiparallel to the rest


α/β
core: 3 layers, a/b/a; mixed beta-sheet of 5 strands, order 12345; strands 2 &, in



some families, 5 are antiparallel to the rest


α/β
Core: 3 layers: a/b/a; mixed beta-sheet of 5 strands, order 21345; strand 5 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 5 strands, order 21345; strand 5 is antiparallel



to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 5 strands, order 32145; strand 2 is antiparallel



to the rest


α/β
core: 3 layers, a/b/a; mixed sheet of 5 strands: order 21354; strand 4 is



antiparallel to the rest; contains crossover loops


α/β
3 layers: a/b/a; mixed beta-sheet of 5 strands; order: 21354, strand 5 is antiparallel



to the rest; permutation of the Phosphorylase/hydrolase-like fold


α/β
3 layers: a/b/a; mixed beta-sheet of five strands, order 21345; strand 1 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 6 strands; order: 213546, strand 5 is



antiparallel to the rest; topological similarity to the MogA-like family fold


α/β
3 layers, a/b/a; core: mixed beta-sheet of 6 strands, order 213456, strand 6 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 6 strands, order 165243, strand 3 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 6 strands, order 126345; strand 1 is



antiparallel to the rest


α/β
core: 3 layers, a/b/a; mixed beta-sheet of 6 strands, order 324156; strand 5 is



antiparallel to the rest


α/β
core: 3 layers, a/b/a; mixed beta-sheet of 6 strands, order 321456; strand 3 is



antiparallel to the rest


α/β
core: 3 layers, a/b/a; mixed beta-sheet of 6 strands, order 321456; strand 3 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 6 strands, order 231456; strand 3 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 6 strands, order 251634; strand 6 is



antiparallel to the rest


α/β
core: 3 layers, a/b/a; mixed beta-sheet of 6 strands, order 432156; strand 4 is



antiparallel to the rest


α/β
core: 3 layers, a/b/a; mixed sheet of 7 strands, order 1237456; strands 1, 6 and 7



are antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 7 strands, order 3214567; strand 6 is



antiparallel to the rest


α/β
core: 3 layers, a/b/a; mixed beta-sheet of 7 strands, order 3214576; strand 7 is



antiparallel to the rest


α/β
3 layers, a/b/a; mixed beta-sheet of 7 strands, order 3214576; strand 7 is



antiparallel to the rest; topological similarity to SAM-dependent



methyltransferases


α/β
main domain: 3 layers: a/b/a, mixed beta-sheet of 7 strands, order 3245671; strand



7 is antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 7 strands, order 3214657; strand 6 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 8 strands, order 32145678; strands 6 and 8 are



antiparallel to the rest


α/β
core: 3 layers, a/b/a; mixed beta-sheet of 8 strands, order 12435678, strand 2 is



antiparallel to the rest


α/β
core: 3 layers, a/b/a; mixed beta-sheet of 8 strands, order 32145687; strand 7 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 8 strands, order 34251687; strand 8 is



antiparallel to the rest


α/β
core: 3 layers: a/b/a; mixed beta-sheet of 8 strands, order 21345678, strand 7 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed (mainly parallel) beta-sheet of 8 strands, order 32145678;



strand 8 is antiparallel to the rest


α/β
3 layers: a/b/a; mixed (mainly parallel) beta-sheet of 8 strands, order 34215786;



strand 8 is antiparallel to the rest


α/β
core: 3 layers: a/b/a; mixed beta-sheet of 8 strands, order 45321678, strands 4 and



5 are antiparallel to the rest


α/β
core: 3 layers: a/b/a; mixed beta-sheet of 8 strands, order 43516728, strand 7 is



antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 8 strands, order 78612354; strands 3, 4 and 8



are antiparallel to the rest


α/β
3 layers: a/b/a; mixed beta-sheet of 9 strands, order 918736452; strands 1, 2 and 8



are antiparallel to the rest


α/β
3 layers: a/b/a; mixed (mostly antiparallel) beta-sheet of 9 strands, order



432159876; left-handed crossover between strands 4 and 5


α/β
3 layers: a/b/a; mixed beta-sheet of 9 strands, order 342156798; strands 3, 8 and 9



are antiparallel to the rest; left-handed crossover connection between strands 6



and 7


α/β
consists of two intertwined (sub)domains related by pseudo dyad; duplication


α/β
possible duplication: the topologies of N- and C-terminal halves are similar; 3



layers: a/b/a; single mixed beta-sheet of 10 strands, order 213549A867 (A = 10);



strands from 5 to 9 are antiparallel to the rest


α/β
consists of two similar domains related by pseudo dyad; duplication


α/β
consists of two similar domains related by pseudo dyad; duplication


α/β
3 layers: a/b/a; parallel beta-sheet of 5 strands, order 21345


α/β
contains of two similar intertwined domains related by pseudo dyad; duplication


α/β
consists of two similar domains with 3 layers (a/b/a) each; duplication


α/β
consists of three similar domains with 3 layers (a/b/a) each; duplication


α/β
consists of three similar domains with 3 layers (a/b/a) each; duplication


α/β
consists of two domains of similar topology, 3 layers (a/b/a) each


α/β
consists of two non-similar domains, 3 layers (a/b/a) each


α/β
consists of two non-similar domains with 3 layers (a/b/a) each


α/β
consists of two non-similar alpha/beta domains, 3 layers (a/b/a) each


α/β
consists of two non-similar domains, 3 layers (a/b/a) each


α/β
consists of two non-similar domains


α/β
consists of two non-similar domains


α/β
2 different domains; d1: [core: 3 layers, a/b/a; parallel sheet of 5 strands, order:



2134]; D2: [2 layers, a/b; mixed sheet of 6 strands, order 321645; strands 2 and 6



are antiparallel to the rest]


α/β
consists of two non-similar domains


α/β
consists of two different alpha/beta domains; (1) of the Flavodoxin-like fold



(scop_cf 52171); (2) similar to the Restriction endonuclease-like fold (scop_cf



52979), inserted into domain 1


α/β
contains a P-loop NTP-binding motif; mixed beta-sheet folds into a barrel-like



structure with helices packed on one side


α/β
contains mixed beta-sheets; topology is partly similar to that of the catalytic C-



terminal domain


α/β
duplication: tandem repeat of two domains; 3 layers (a/b/a); parallel beta-sheet of



4 strands, order 2134


α/β
consists of two similar intertwined domain with 3 layers (a/b/a) each: duplication


α/β
consists of two similar intertwined domain with 3 layers (a/b/a) each: duplication


α/β
consists of two similar domains related by pseudo dyad; duplication


α/β
consist of two intertwined domains; duplication: contains two structural repeats of



alpha-beta-(beta-alpha)3 motif with mixed beta-sheet, order: 1432, strand 1 is



antiparallel to the rest


α/β
consist of two intertwined domains; contains partial duplication


α/β
consist of two different alpha/beta domains; N-terminal domain has a SurE-like



topology with a left-handed beta-alpha-beta unit


α/β
core: alpha-beta(2)-(alpha-beta)2; 3 layers (a/b/a); mixed beta-sheet of 4 strands,



order 2134; strand 2 is antiparallel to the rest


α/β
single helix packs against antiparallel beta-sheet


α/β
common alpha + beta motif for the active site region


α/β
consists of one alpha-helix and 4 strands of antiparallel beta-sheet and contains



the catalytic triad Cys-His-Asn


α/β
core: (alpha)-beta-omega_loop-beta-alpha; embeded in larger different structures


α/β
contains long curved beta-sheet and 3 helices


α/β
beta-alpha-beta-alpha(2); antiparallel beta-ribbon


α/β
beta-alpha(2)-beta; antiparallel strands


α/β
alpha-beta(2)-alpha; antiparallel hairpin


α/β
alpha-beta(2)-alpha; 2 layers a/b; antiparallel beta-hairpin


α/β
alpha(3)-beta(2); antiparallel hairpin


α/β
beta(3)-alpha


α/β
beta(3)-alpha; 2 layers: alpha/beta


α/β
alpha1-beta3; 2 layers: alpha/beta; order 132


α/β
beta-alpha-beta(2); 2 layers: alpha/beta; antiparallel beta-sheet: order 132


α/β
beta-(alpha)-beta-alpha-beta(2); 3 layers: alpha/beta/alpha; antiparallel beta-sheet:



order 1243


α/β
beta-(2)-alpha(2)-beta(2); 2 layers: beta/alpha; antiparallel beta-sheet: order 1243;



topological similarity to the common core of ribosomal proteins L23 and L15e


α/β
beta-(2)-alpha(3)-beta(2); 2 layers: beta/alpha; mixed beta-sheet: order 1234;



strands 2 and 3 a parallel to each other


α/β
alpha-beta(3)-alpha-beta(2); 3 layers: alpha/beta/alpha


α/β
alpha-beta(3)-alpha-beta(2)-alpha; 2 layers: alpha/beta


α/β
beta(2)-alpha(2)-beta; 2 layers: 3-stranded antiparallel beta-sheet, order 213; HTH



motif; also includes the extra N-terminal, DNA minor groove-binding helix


α/β
alpha-beta(4)-alpha-beta(2)-alpha; 2 layers: alpha/beta


α/β
beta(4)-alpha-beta(2)-alpha; 2 layers: alpha/beta; antiparallel beta-sheet, order:



651234


α/β
core: beta(3)-alpha-beta-alpha; 2 layers: alpha/beta; left-handed crossover


α/β
core: beta(2)-alpha-beta(2); mixed beta-sheet 2143


α/β
alpha + beta sandwich


α/β
Core: alpha-beta(4); helix packs against coiled antiparallel beta-sheet


α/β
alpha-beta-alpha-beta-alpha(2)-beta(3); antiparallel beta-sheet; order: 15432


α/β
alpha(2)-beta(4)-alpha, 2 layers: alpha/beta, antiparallel beta sheet, meander


α/β
beta(3)-alpha-beta(2)-alpha; 2 layers, alpha/beta; antiparallel beta-sheet, order:



12543


α/β
core: alpha-beta(3)-alpha, 2 layers: alpha/beta, three-stranded antiparallel beta



sheet, strand order 123


α/β
core: beta(2)-alpha(2), 2 layers: alpha/beta; long C-terminal helix forms dimeric



parallel and tetrameric antiparallel coiled coils


α/β
helix-swapped dimer of beta(4)-alpha motifs


α/β
beta-BETA(2)-beta-alpha-beta(2); antiparallel sheet: order 2134 packed against



helix and BETA-hairpin on the same side; irregular C-terminal tail


α/β
Dimeric


α/β
alpha-beta(4)-alpha(3); core: meander beta-sheet plus one helix 2


α/β
core: three short helices packed against a barrel-like beta-sheet; some similarity to



the SH3-like fold


α/β
beta*-alpha-beta(2)-alpha-beta-alpha; mixed beta sheet forms a partly open



barrel: (n* = 4, S* = 8)


α/β
beta-alpha-beta(4)-alpha-beta(2); contains beta-sheet barrel (n = 5, S = 8)


α/β
beta(3)-alpha(2)-beta; 2 layers; mixed beta-sheet, order 4123, strands 1 and 4 are



parallel to each other


α/β
mixed beta-sheet folds into a barrel (n = 8, S = 14) around the central helix


α/β
beta-sheet folds into a barrel (n = 11, S = 14) around the central helix


α/β
beta-sheet folds into a barrel (n = 12, S = 12) around the central helix


α/β
contains very long N-terminal helix, which end is packed against beta-sheet


α/β
core: beta(7)-alpha(2); N- and C-terminal extensions form a coiled coil



subdomain


α/β
beta(6)-alpha; antiparallel beta-sheet, meander


α/β
beta(3)-alpha-beta(3)-alpha; 3 layers a/b/a


α/β
alpha(2)-beta(5)-alpha(2); 3 layers a/b/a; meander beta-sheet


α/β
core: beta(2)-alpha-beta(2); antiparallel beta-sheet


α/β
beta(4)-alpha-beta; 2 layers: alpha/beta; mixed beta-sheet, order: 51234


α/β
alpha-beta-X-beta(2); 2 layers: alpha/beta; mixed beta-sheet, order: 123


α/β
beta-alpha-beta-(alpha)-beta(2); 2 layers: alpha/beta; mixed beta-sheet, order:



1342


α/β
beta(2)-alpha-beta; 2 layers: alpha/beta


α/β
beta-alpha-beta(3); 2 layers: alpha/beta


α/β
beta-alpha-beta(3); 2 layers: alpha/beta


α/β
beta(2)-alpha-beta(3); 2 layers: alpha/beta


α/β
multiple repeats of beta(2)-alpha(2) motif


α/β
beta(2)-alpha(3)-beta; two layers: alpha/beta; antiparallel sheet: order 213


α/β
beta(4)-alpha(2); two layers: alpha/beta; antiparallel sheet: order 1432


α/β
beta(2)-alpha(2)-beta(2)-alpha-beta; two layers: alpha/beta; antiparallel sheet:



order 51234


α/β
beta-alpha(2)-beta(4)-alpha-beta(2); two layers: alpha/beta; bifurcated coiled



beta-sheet: order of the first 5 strands: 23154


α/β
beta(4)-alpha(2)-beta(2)-alpha; antiparallel sheet: order 123465


α/β
beta-alpha-beta(6)-alpha(2); antiparallel sheet: order 165432


α/β
beta(3)-alpha(2)-beta-alpha(2)-beta3; 2 layers alpha/beta; antiparallel sheet: order



1234567


α/β
alpha-beta(6)-alpha(2)-beta-alpha(n); 3 layers alpha/beta/alpha; antiparallel sheet:



order 1234567


α/β
beta(4)-alpha-beta(2)-alpha(2); mixed, predominately antiparallel beta-sheet,



order: 123465, strands 4 and 5 are parallel to each other


α/β
core: beta-alpha-beta(4); 2 layers: alpha/beta


α/β
core: beta-alpha-beta(4); 2 layers: alpha/beta


α/β
core: beta-alpha(2)-beta-X-beta(2); 2 layers: alpha/beta; antiparallel beta-sheet:



order 1342


α/β
alpha + beta sandwich; loop across free side of beta-sheet


α/β
alpha-beta-loop-beta(3); loop across free side of beta-sheet


α/β
core: beta-BETA-alpha-beta-BETA-beta-alpha; contains a beta-hammerhead



motif similar to that in barrel-sandwich hybrids


α/β
core: beta(2)-alpha(2)-beta(2)-alpha(2); 2 layers a/b; mixed sheet: 2143


α/β
beta(2)-alpha(n)-beta; 2 layers a/b; antiparallel sheet: 123


α/β
alpha-beta(2)-alpha-beta-alpha(2); 3 strands of antiparallel sheet: 213


α/β
beta-alpha(2)-beta-alpha-beta; 2 layers, alpha/beta


α/β
beta-alpha-beta(2)-alpha(2); 3 layers, alpha/beta/alpha; antiparallel beta-sheet:



order 123


α/β
beta-alpha(2)-beta(2); 2 layers, alpha/beta; antiparallel beta-sheet: order 123


α/β
alpha-beta(3)-alpha(2); 2 layers, alpha/beta


α/β
(beta)-alpha-beta(3)-alpha; 2 layers, alpha/beta


α/β
alpha-beta(3)-alpha; 2 layers: alpha/beta


α/β
duplication: consists of two beta(3)-alpha repeats; 3 layers, beta/alpha/beta


α/β
beta-alpha-beta(2)-alpha; 2 layers: alpha/beta


α/β
alpha(2)-beta(3)-alpha(3); 2 layers alpha/beta, 3-stranded antiparallel beta-sheet;



order 123


α/β
alpha(3)-beta-alpha(2)-beta(2); 2 layers alpha/beta, 3-stranded antiparallel beta-



sheet; order 123


α/β
beta-alpha(2)-beta(2)-alpha; 2 layers: alpha/beta


α/β
core: alpha-beta(2)-(alpha)-beta; 2 layers: alpha/beta


α/β
core: alpha-beta-turn-beta-X-beta-(alpha); mixed beta-sheet, order of core strands:



123


α/β
alpha(2)-beta(4); 2 layers: alpha/beta; antiparallel beta-sheet: order 2143


α/β
alpha-beta(3)-alpha-beta-alpha; bifurcated coiled beta-sheet


α/β
beta(3)-alpha(3); meander and up-and-down bundle


α/β
beta-alpha(3)-beta(2); 2 layers: alpha/beta; related to the enolase/MLE N-domain



fold by a circular permutation


α/β
alpha-beta-alpha(3)-beta(2); 2 layers: alpha/beta;


α/β
3-helical bundle packed against 3-stranded mixed beta-sheet


α/β
beta(3)-alpha(4); meander beta-sheet packed against array of helices; contains



Pro-rich stretch


α/β
beta(3)-alpha(5); meander beta-sheet packed against array of helices


α/β
beta-alpha-beta(2)-alpha; 2 layers: alpha/beta; mixed sheet 213; crossing loops


α/β
alpha-beta(3)-alpha(3); 2 layers, a/b; mixed beta-sheet, order: 132; crossing loops


α/β
alpha + beta sandwich with antiparallel beta-sheet; (beta-alpha-beta) × 2


α/β
consists of two alpha + beta subdomains with some similarity to the ferredoxin-like



fold


α/β
beta-alpha-beta-X-beta(2)-alpha(2)-beta; antiparallel beta-sheet, order 24153;



topological similarity to the ferredoxin-like fold (scop_cf 54861)


multi
contains a cluster of helices and a beta-sandwich


multi
contains a cluster of helices and a beta-sandwich


multi
contains a cluster of helices and an alpha + beta sandwich


multi
consists of an all-alpha and alpha + beta domains


multi
contains a helical bundle with a buried helix and an alpha + beta insert domain


multi
consists of an all-alpha and alpha + beta domains connected by antiparallel coiled



coil


multi
contains a cluster of helices and an alpha/beta domain


multi
contains an (8, 10) beta-barrel and an all-alpha domain


multi
2 domains: (1) all-alpha: 5 helices; (2) contains an open beta-sheet barrel: n* = 5,



S* = 8; complex topology


multi
N-terminal domain is an alpha + beta, C-terminal domain is an alpha/beta with



mixed beta-sheet


multi
divided into morphological domains including “palm”, “thumb” and “fingers”; the



catalytic “palm” domain is conserved to all members


multi
Multidomain subunits of complex domain organization


multi
3 domains: (1&2) alpha + beta, with domain 2 being inserted in domain 1; (3) all-



alpha


multi
4 domains: (1) Toprim alpha/beta; (2&4) “winged helix”-like; (3) barrel: n = 6,



S = 8


multi
4 domains: (1) toprim alpha/beta; (2) “winged helix”-like; (3) alpha + beta; (4) all-



alpha


multi
2 domains: (1) toprim alpha/beta; (2) “winged helix”-like


multi
2 domains: (1) alpha + beta; (2) toprim alpha/beta


multi
consists of three domains: alpha-helical dimerisation domain (res. 1-53) with



HhH motif (Pfam 00633); ‘treble cleft’ C4 zinc-finger domain (54-76; Pfam



02132); and Toprim domain (76-199; segment-swapped dimer; Pfam 01751)


multi
2 domains: alpha + beta and all-beta


multi
2 domains: (1) alpha + beta: beta3-alpha2-beta2; (2) alpha/beta, a part of its mixed



sheet forms barrel: n = 6, S = 8


multi
3 domains: (1) all-alpha; (2&3) alpha + beta


multi
2 domains: (1) alpa/beta; (2) Fe—S cluster-bound


multi
2 domains: (1) alpha/beta of a Rossmann-fold topology, binds NAD (2)



multihelical array


multi
4 domains: (1&2) duplication: share the same alpha/beta fold; (3) beta-barrel; (4)



alpha + beta


multi
2 domains: (1) alpha + beta; (2) alpha/beta (interrupts domain 1)


multi
4 domains: (1) 3-helical bundle; (2) alpha + beta of ferredoxin-like fold (3 and 4)



alpha + beta of dsRDB-like fold


multi
3 domains: (1) 3-helical bundle; (2 and 3) alpha + beta of different folds: domain 3



has a ferredoxin-like fold and is inserted in domain 2


multi
3 domains: (1) 4-helical bundle; (2) alpha + beta; (3) “winged helix”-like


multi
3 domains: (1 and 2) alpha + beta; (3) mostly alpha, inserted in domain 2


multi
3 domains: (1) spectrin repeat-like 3-helical bundle; (2 and 3) alpha/beta:



Rossmann-fold topology


multi
3 domains: (1) protozoan pheromone-like alpha-helical bundle; (2) rubredoxin-



like domain lacking metal-binding site; (3) alpha + beta heterodimerisation



domain: alpha-beta(5)-alpha


multi
2 domains: (1) alpha-helical bundle; (2) beta-barrel (n = 5, S = 8)


multi
3 domains: (1) alpha-helical bundle; (2&3) complex all-beta folds


multi
2 closely associated domains: (1) all-alpha, EF-hand like; (2) alpha + beta,



Frataxin-like


multi
2 domains; d1: [all-alpha; 3-helical bundle, similar to the



immunoglobulin/albumin-binding domain-like fold (scop_cf 46996)]; d2:



[alpha/beta; 3 layers, a/b/a; 6-stranded mixed beta-sheet, order: 321456, strand 6



is antiparallel to the rest]


multi
3 domains; d1: alpha + beta [alpha(2)-beta(3); mixed sheet: 213]; d2: alpha/beta of



the NAD(P)-binding Rossmann-fold superfamily (scop_sf 51735, most similar to



scop_fa 51883 and scop_fa 51736); d3: alpha + beta of the glutamine



synthetase/guanido kinase fold (scop_cf 55930); d1 and d3 form a single beta-



sheet


multi
2 domains: d1 [alpha/beta; related to the PFK N-terminal domain (scop_sf



53784)]; d2 [all-beta; atypical beta-sandwich made of 4 structural repeats of



beta(3) unit]


multi
2 domains; d1 (1-64, 174-335) [alpha/beta; 3 layers, a/b/a; mixed beta sheet of 9



strands, order: 219863457; strands 1, 5 and 8 are antiparallel to the rest]; d2 (65-142)



[all-beta; barrel, closed (n = 6, S = 10); greek-key; topologically similar to the



split barrel fold (scop_cf 50474)


multi
2 domains; (1) alpha + beta (res 1-192), a circularly permuted rS5 domain 2-like



fold (scop_cf 54210); (2) alpha/beta with parallel beta-sheet of 4 strands, order



2134


multi
consists of two domains; d1: alpha + beta (78-190; alpha-beta(4)-alpha-beta-alpha;



3 layers; antiparallel beta-sheetof 5 strands; order 51234); d2: alpha/beta similar



to the G-domain fold (191-381; scop_fa 52592)


multi
2 domains: (1) all-alpha, (2) alpha + beta; asymmetric homodimer with each



domain intertwining with its counterpart


multi
4 domains: three intertwined predominately alpha domains and one jelly-roll beta-



sandwich


multi
large protein without apparent domain division; has a number of all-alpha regions



and one all beta domain near the C-end


multi
large protein without apparent domain division


multi
large protein without apparent domain division


membrane +
multi-helical domains of various folds which unfold in the membrane


surface


membrane +
core: up-and-down bundle of seven transmembrane helices tilted 20 degrees with


surface
respect to the plane of the membrane


membrane +
five transmembrane helices forming a sheet-like structure


surface


membrane +
12 transmembrane helices in an approximate threefold rotational symmetric


surface
arrangement


membrane +
core: 7 transmembrane helices organized into two bundles, one formed by the


surface
first two helices and the other by the rest


membrane +
two antiparallel transmembrane helices


surface


membrane +
core: up-and-down bundle of four transmembrane helices


surface


membrane +
core: 8 helices, 2 short helices are surrounded by 6 long transmembrane helices


surface


membrane +
11 transmembrane helices; duplication: consist of 2 structural repeats of five


surface
helices each plus extra C-terminal helix


membrane +
12 transmembrane helices; duplication: the N- and C-terminal halves are


surface
structurally similar


membrane +
core: 18 transmembrane helices


surface


membrane +
oligomeric transmembrane alpha-helical proteins


surface


membrane +
oligomeric transmembrane alpha-helical protein


surface


membrane +
oligomeric transmembrane alpha-helical protein


surface


membrane +
heteropentameric transmembrane alpha-helical protein; 4 transmembrane helices


surface
per subunit


membrane +
oligomeric fold; 3 transmembrane helices per subunit


surface


membrane +
oligomeric fold; 3 transmembrane helices per subunit


surface


membrane +
9 transmembrane helices


surface


membrane +
10 transmembrane helices forming of a gated channel


surface


membrane +
core: 11 transmembrane helices


surface


membrane +
core: hairpin of two transmembrane helices


surface


membrane +
core: three transmembrane helices, bundle


surface


membrane +
multihelical; complex architecture with several transmembrane helices


surface


membrane +
multihelical; complex architecture with several transmembrane helices


surface


membrane +
12 transmembrane helices; duplication: the N- and C-terminal halves of the whole


surface
proteins are structurally similar


membrane +
core: three transmembrane helices, up-and-down bundle


surface


membrane +
core: four transmembrane helices, up-and-down bundle, binds one or two heme


surface
groups in between the helices


membrane +
membrane-associated alpha-helical protein; no transmembrane helices


surface


membrane +
membrane-associated alpha-helical protein; no transmembrane helices


surface


membrane +
2 helices, hairpin


surface


membrane +
core: multihelical; consists of three transmembrane regions of 2, 2 and 6 helices,


surface
separated by cytoplasmic domains


membrane +
membrane all-alpha fold


surface


membrane +
membrane all-alpha fold; 6-helical “barrel” with internal binding cavity


surface


membrane +
membrane all-alpha fold; three transmembrane helices


surface


membrane +
, gathers together transmembrane barrels of different (n, S)


surface


membrane +
subunit fold contains tandem repeat of alpha-beta hairpin-alpha(2) motif


surface


membrane +
consists of three domains: beta-barrel (res. 29-38, 170-259; scop_cf 50412);


surface
barrel-sandwich hybrid (39-72, 135-169; scop_sf 51230) and long alpha-hairpin



(73-134; scop_cf 46556)


membrane +
subunit fold contains beta-sandwich of Ig-like (grerk-key) topology and a beta-


surface
ribbon arm that forms an oligomeric transmembrane barrel


membrane +
contains several large open beta-sheets


surface


membrane +
3 domains: (1) alpha + beta; (2&3) all-beta


surface


membrane +
2 domains: (1) alpha + beta; (2) all-beta, similar to the CalB domain fold but the


surface
two last strands are transposed


membrane +
2 intertwined domains; all-beta and alpha + beta


surface


membrane +
2 domains; d1: complexed all-beta fold; d2: coiled-coil (trimeric) helical region


surface


membrane +
3 intertwined all-beta domains


surface


membrane +
trimer; one subunit consists of an alpha/beta oligomerization subdomain [3-


surface
stranded parallel beta-sheet, order 213], and an antiparallel coiled coil


membrane +
4 domains; I (res. 14-225) and II (226-487) are beta-sandwiches of similar


surface
gamma-crystallin like topologies; III (488-594) has a beta-grasp like fold; IV



(595-735) has an Ig-like fold


Other
nearly all-alpha


Other
disulfide crosslinked alpha-helical hairpin


Other
disulfide-bound fold; contains beta-hairpin with two adjacent disulfides


Other
disulfide-rich fold; all-beta: 3 antiparallel strands


Other
disulfide-rich fold; all-beta: 3 antiparallel strands


Other
disulfide-rich fold; all-beta: 3 antiparallel strands


Other
disulfide-rich; alpha + beta: 3 antiparallel strands followed by a short alpha helix


Other
disulfide-rich fold: nearly all-beta


Other
disulfide-rich alpha + beta fold


Other
Disulfide-rich fold, nearly all-beta


Other
alpha + beta fold with two crossing loops


Other
disulfide-rich fold


Other
disulfide-rich calcium-binding fold


Other
disulfide-rich alpha + beta fold


Other
disulfide-rich fold; nearly all-beta


Other
disulfide-rich small alpha + beta fold; topological similarity to the Ovomucoid



domain III


Other
disulfide-rich fold; common core is alpha + beta with two conserved disulfides


Other
disulfide-rich fold; all-beta; duplication: contains two structural repeats


Other
disulfide-rich fold; common core is all-beta


Other
disulfide-rich all-beta fold


Other
disulfide-rich all-alpha fold


Other
small disulfide-rich


Other
disulfide-rich; nearly all-beta


Other
disulfide-rich; nearly all-beta


Other
disulfide-rich; alpha + beta


Other
duplication: consists of three similar disulfide-rich domains


Other
duplication: consists of two similar disulfide-rich domains, alpha + beta


Other
disulfide-rich; all-beta: open barrel, 5 strands; OB-fold-like


Other
disulfide-rich, all-beta


Other
disulfide-rich, alpha + beta


Other
disulfide-rich, alpha + beta


Other
disulfide-rich, alpha + beta


Other
disulfide-rich, alpha + beta


Other
disulfide-rich


Other
disulfide-rich, all-alpha


Other
disulfide-rich; all-alpha


Other
disulfide-rich, alpha + beta


Other
disulfide-rich


Other
disulfide-rich; all-alpha; calcium-binding


Other
disulfide-rich


Other
disulfide-rich all-beta fold; contains beta sandwich of 5 strands


Other
disulfide-rich six-stranded beta-sandwich; jelly-roll


Other
bipartite cysteine-rich all-alpha domain; a single helix in the N-terminal part



(chain A) is linked by disulfides to the C-terminal part (chain B) [3-helical bundle



of the RuvA C-terminal domain-like fold (scop_cf 46928)


Other
Calcium ion-bound


Other
a few helical turns and a disulfide-crosslinked loop


Other
a few helical turns assembled without a hydrophobic core?


Other
folds around 4Fe—4S cluster


Other
folds around 4Fe—4S cluster


Other
alpha + beta metal(zinc)-bound fold: beta-hairpin + alpha-helix


Other
all-alpha dimetal(zinc)-bound fold


Other
alpha + beta metal(zinc)-bound fold


Other
consist of two different zn-binding subdomains, each subdomain resembles a



distorted glucocorticoid receptor-like fold


Other
metal(zinc)-bound fold


Other
metal(zinc or iron)-bound fold; sequence contains two CX(n)C motifs, in most



cases n = 2


Other
zinc-bound beta-ribbon motif


Other
zinc-bound beta-ribbon motif


Other
zinc-bound alpha + beta motif


Other
dimetal(zinc)-bound alpha + beta motif; structurally diverse


Other
zinc-bound alpha + beta motif


Other
metal(iron)-bound fold


Other
metal(zinc)-bound alpha + beta fold


Other
metal(zinc)-bound alpha + beta fold


Other
dimetal(zinc)-bound alpha + beta fold


Other
dimetal(zinc)-bound alpha + beta fold


Other
metal(zinc)-bound alpha + beta fold


Other
metal(zinc)-bound alpha + beta fold


Other
metal(zinc)-bound alpha + beta fold


Other
Zn-binding, all-alpha fold


Other
all-alpha fold; Zn-binding sites are in the loops connecting helices


Other
alpha-helical fold with two Zn-binding sites


Other
metal(zinc)-bound extended beta-hairpin fold


Other
metal(zinc)-bound fold


Other
metal(zinc)-bound fold


Other
metal(calcium)-bound fold









Terms used in Table 1 will be apparent to the skilled artisan. However, the following definitions are provided for clarity below.


“Meander” is a simple topology of a beta-sheet where any two consecutive strands are adjacent and antiparallel.


“Up-and-down” is the simplest topology for a helical bundle or folded leaf, in which consecutive helices are adjacent and antiparallel; it is approximately equivalent to the meander topology of a beta-sheet.


“Crossover connection” links secondary structures at the opposite ends of the structural core and goes across the surface of the domain.


“Greek-key” is a topology for a small number of beta sheet strands in which some interstrand connections going across the end of barrel or, in a sandwich fold, between beta sheets.


“Jelly-roll” is a variant of Greek key topology with both ends of a sandwich or a barrel fold being crossed by two interstrand connections.


“All-alpha” class has the number of secondary structures in the domain or common core described as 3-, 4-, 5-, 6- or multi-helical.


“Bundle” is an array of alpha-helices each oriented roughly along the same (bundle) axis. It may have twist, left-handed if each helix makes a positive angle to the bundle axis, or be right-handed if each helix makes a negative angle to the bundle axis.


“Folded leaf” is a layer of alpha-helices wrapped around a single hydrophobic core but not with the simple geometry of a bundle.


“Array” (of hairpins) is an assembly of alpha-helices that can not be described as a bundle or a folded leaf.


“Closed”, “partly opened” and “opened” for all-alpha structures describes the extent in which the hydrophobic core is screened by the comprising alpha-helices. “Opened” means that there is space for at least one more helix to be easily attached to the core:


Beta-sheets can be “antiparallel” (i.e. the strand direction in any two adjacent strands are antiparallel), “parallel” (all strands are parallel each other) or “mixed” (there is one strand at least that is parallel to one of its two neighbours and antiparallel to the other).


“All-beta” class includes two major fold groups: sandwiches and barrels. The “sandwich” folds are made of two beta-sheets which are usually twisted and pack so their strands are aligned. The “barrel” fold are made of single beta-sheet that twists and coils upon itself so, in most cases, the first strand in the beta sheet hydrogen bond to the last strand. The strand directions in the two opposite sides of a barrel fold are roughly orthogonal. Orthogonal packing of sheets is also seen in a few special cases of sandwich folds


“Barrel structures” are usually closed by main-chain hydrogen bonds between the first and last strands of the beta sheet, in this case it is defined by the two integer numbers: the number of strand in the beta sheet, n, and a measure of the extent the extent to which the strands in the sheet are staggered the shear number, S.


“Partly open barrel” has the edge strands not properly hydrogen bonded because one of the strands is in two parts connected with a linker of more than one residue. These edge strands can be treated as a single but interrupted strand, allowing classification with the effective strand and shear numbers, n* and S*. In the few open barrels the beta sheets are connected by only a few side-chain hydrogen bonds between the edge strands.


It is likely that there exists a bias in nature towards particular folds, simply because of the evolutionary constraints applied to protein structure and function determination. For example, approximately 30% of folds and 50% of protein superfamilies are contained within about 4-5 architectures, in particular αβ-sandwiches (two- and three-layer), αβ-barrel, β-barrel, α-updown structures (see Orengo et al., Ann. Rev. Biochem. 74, 867-900, 2005). Many folds are also reported as sharing common structural motifs due to the recurrence of simple structural motifs e.g., αβ-motifs, ββ-motifs, split βαβ-motifs. Nearly 80 different folds are classified as adopting a three-layer αβ-sandwich architecture, and the most highly-populated fold groups adopt regular architectures (e.g., TIM barrel fold, αβ-barrel, Rossman fold; three-layer, αβ-sandwich; αβ-plait, two-layer αβ-sandwich) that may be more stable when mutated (Orengo et al., ibid.). Recent statistical analyses suggest that more highly-represented folds i.e., “superfolds” support a much broader repertoire of primary sequences than other folds (Shakhnovich et al., J. Mol. Biol. 326, 1-9, 2003). For example, the CATH database provides a hierarchical classification of domains, within protein structures, in the Protein Data Bank (PDB; Berman et al., Nucl. Acids Res. 28, 235-242, 2000). There are about 32 architectures described in the CATH database.


5. Peptide Sources


Methods for producing libraries encoding peptides that correspond to naturally-occurring protein domains and/or sub-domains and/or are capable of forming secondary and/or super-secondary structures are known e.g., as described in International Patent Publication Nos. WO/2004/074479 (International Application No. PCT/AU2004/000214) and WO/2007/097923 (International Application No. PCT/AU2007/097923). The contents of these applications are incorporated herein in their entirety.


For example, nucleic acid fragments comprising genomic DNA, cDNA, or amplified nucleic acid derived from one or two or more well-characterized genomes e.g., a prokaryote genome or a eukaryote having a small genome such as a protist, dinoflagellate, alga, plant, fungus, mould, invertebrate or vertebrate may be employed to produce an expression library. Such nucleic acid fragments are derived, for example, from one or two or more of Aeropyrum pernix, Aquifex aeolicus, Archaeoglobus fulgidis, Bacillus subtilis, Bordetella pertussis, Borrelia burgdorferi, Chlamydia trachomatis, Escherichia coli, Haemophilus influenzae, Helicobacter pylori, Methanobacterium thermoautotrophicum, Methanococcus jannaschii, Mycoplasma pneumoniae, Neisseria meningitidis, Pseudomonas aeruginosa, Pyrococcus horikoshii, Synechocystis PCC 6803, Thermoplasma volcanium and Thermotoga maritima. The nucleic acid fragments are generated using art-recognized methods e.g., mechanical shearing, digestion with a nuclease, digestion with a restriction endonuclease, amplification by polymerase chain reaction (PCR) using random oligonucleotide primers, and combinations thereof.


The nucleic acid fragments are inserted into a suitable expression vector or gene construct in operable connection with a suitable promoter for expression of an encoded peptide in each clone. One approach employs site-specific recombinases to integrate fragments comprising one or two flanking recombination sites into a plasmid vector having compatible recombination sites. Site-specific recombination systems typically comprise one or more proteins that recognize a specific recombination site sequence in a plasmid vector and in the DNA insert, cleave the nucleic acids and ligate them together via cross-over event(s). Several site-specific recombinases are known in the art e.g., the bacteriophage P1 Cre/lox system (Austin et al. Cell 25, 729-736, 1981), the R/RS recombinase system from the pSRi plasmid of the yeast Zygosaccharomyces rouxii (Araki et al., J. Mol. Biol. 182, 191-203, 1985), the Gin/gix system of phage Mu (Maeser et al., Mol. Gen. Genet. 230, 170-176, 1991), the FLP/FRT recombinase system from the 2 micron plasmid of the yeast Saccharomyces cerevisiae (Broach et al.; Cell 29, 227-234, 1982), and the Integrase from bacteriophage Lambda (Landy et al., Ann. Rev. Biochem. 58, 912-949, 1989; Landy et al., Curr. Opin. Genet. Dev. 3, 699-707, 1993; Lorbach et al., J. Mol. Biol. 296, 1175-1181, 2000; and WO 01/16345). The integrase system utilizes attachment sites (attB, attP, attL, attR) to facilitate integration of insert nucleic acid into vector, wherein attB sites recombine with attP sites in a reaction mediated by an integrase enzyme to yield attL and attR sites on resulting “entry” plasmid vectors. The DNA inserts are then mobilized into a suitable “destination” expression plasmid by recombination between attL sites and attR sites in a reaction mediated by an integrase enzyme to yield attB and attP sites.


Serine recombinase systems e.g., Sin resolvase system, are also known in the art to provide for recombination between donor and acceptor sites in DNA for cloning purposes. For example, the Mycobacterium tuberculosis prophage-like element ΦRv1 encodes a site-specific recombination system utilizing an integrase of the serine recombinase family, wherein recombination occurs between a putative attP site and the host chromosome, but is unusual in that the attB site lies within a redundant repetitive element (REP13E12) of which there are seven copies in the M. tuberculosis genome; and wherein four of these repetitive elements contain attB sites suitable for ΦRv1 integration in vivo. Although the mechanism of directional control of large serine integrases is poorly understood, a recombination directionality factor (RDF) has been identified that is required for ΦRv1 integrase-mediated excisive recombination in vivo. Defined in vitro recombination reactions for both ΦRv1 integrase-mediated integration and excision require the ΦRv1 RDF for excision, but not DNA supercoiling, host factors, or high-energy cofactors (unlike the lambda integrase system). Integration, excision and excise-mediated inhibition of integration require simple substrates sites, indicating that the control of directionality does not involve the manipulation of higher-order protein-DNA architectures as described for the tyrosine integrases.


Generally, the construct used for expression is determined by the system(s) that will be used to display the encoded peptides for screening purposes e.g., by direct display on a physical medium or by phage display or recombinant expression. Such display generally provides for the peptides to assume a secondary or super-secondary structure.


Alternatively, peptide libraries are produced based on source data comprising annotations of primary sequences determined and/or predicted structures for proteins from which the component peptides are derived. For example, source data consisting of protein sequence resources such as PRINTS, Pfam, SMART, Propom, InterPro, TIGRFAMs, ADDA, CHOP, ProtoNet, SYSTERS, iProClass, SWISSPROT, COG/KOG, and protein structure family resources such as CAMPASS (Cambridge University, UK), CATH database (University College, London, UK), CE (SDSC, La Jolla, Calif., USA), DHS (University College, London, UK), ENTREZ/MMDB (NCBI, Bethesda Md., USA), Structural Classification of Protein Database (SCOP) (Andreeva et al., Nucl. Acid Res. 32:D226-D229, 2004), or the Protein Data Bank (PDB) (Berman et al., Nucleic Acid Res. 28: 235, 2000) are used to determine amino acid sequences capable of independently-forming secondary structures and/or assemblies of secondary structures and/or folds suitable for practical application in drug screening. In such an approach, synthetic peptides are produced having the sequences that are capable of forming those secondary structures and super-secondary structures, or alternatively, nucleic acid encoding the amino acid sequences are synthesized and cloned into suitable expression vectors as described herein above. As with libraries produced from genomic fragments, peptide libraries produced using bioinformatics data must be displayed for the purposes of screening to ascertain their bioactivity. Again, display generally provides for the peptides to assume a secondary or super-secondary structure.


In the foregoing methods, each clone of the library encodes, on average, a monomeric peptide.


Suitable display methods for peptide libraries include e.g., arraying the peptides on a solid surface, e.g., a microarray, or on a plurality of solid surfaces, e.g., a plurality of beads, or in microwells. The peptides may be synthesized directly onto a solid surface or immobilized on a solid surface. For example, a parallel array or pool of peptides can be produced by synthetic means and arrayed in a multi-well plate for high-throughput screening. Peptides can also be displayed using recombinant means e.g., by virtue of being expressed on the surface of a phage or a cell or by ribosome display or by in vitro display or within cells. In such methods, peptides are generally displayed (and subsequently screened) as monomers.


Modulators of CD40/CD40L Signaling

Monoclonal antibodies that block the interaction of CD40L with its cognate CD40 receptor to prevent allograft rejection in primates have been described e.g., Kirk et al., Nature Med. 5, 686-693 (1999). Such immunotherapy has also been reported for therapy of animal models of diabetes e.g., Kover et al., Diabetes 49, 1666-1670 (2000) and atherosclerosis e.g., Mach et al., Nature 394, 200-203 (1998). CD40L immunotherapy carries a high incidence of adverse consequences such as thromboembolic complications e.g., Boumpas et al., Arthrtitis Rheum. 48, 719-727 (2003), possibly due to the induction of Fc-mediated platelet aggregation e.g., Langer et al., Thromb Haemost 93, 1137-1146 (2005); Mirabet et al., Mol. Immunol. 45, 937-944 (2008).


A peptide derived from the native CD40-CD40L interface i.e., residues 181-205 of CD40L and a retro-inverso peptide analog thereof are described by Allen et al., J. Peptide Res. 65, 591-604 (2005). The term “native interface” or “native CD40-CD40L interface” or similar means that the peptide comprises a linear sequence of one of the binding partners i.e., CD40 or CD40L that is involved in their interaction, or a reverse sequence thereof e.g., a sequence of a retro-inverted analog. For example, the peptide described by Allen et al. (2005) comprises 8203 of CD40L known to be involved in binding to CD40 and flanking sequence. The peptide and its chiral analog were reported to block T-cell proliferation in vitro, and to reduce incidence and severity of experimental encephalomyelitis (EAE) when administered to mice. There are a limited number of primary or secondary or tertiary structure permutations derivable the native interaction interface of CD40 and CD40L, thereby limiting the range of available therapeutics for ameliorating the adverse consequences of CD40-signaling through CD40L.


More recently, phage-expressed 7-mer peptide aptamers that had been disulfide-constrained by cyclization through their N-terminal and C-terminal cysteine residues, have also been described to bind to CD40L in vitro, and a single peptide thereof shown to inhibit CD40-mediated B-cell activation, Ig switching, endothelial cell migration and angiogenesis e.g., Deambrosis et al., J. Mol. Med. 87, 181-197 (2009). As with strategies for peptidomimetics based on the native CD40-CD40L interface, strategies employing disulfide-constrained aptamers of fixed length form a limited number of secondary or tertiary structure permutations, thereby limiting the range of available therapeutics for ameliorating the adverse consequences of CD40-signaling through CD40L. This conclusion is supported by the low primary hit, rate in aptamer screens for binding activity and/or high attrition rate of aptamers tested for inhibitory activity.


There is an ongoing need for compounds that ameliorate the adverse effects of CD40L signaling events, including those events mediated by CD40 and/or Mac-1. Inverse agonists and antagonists of CD40L would be particularly useful for providing such benefits.


General

Conventional techniques of molecular biology, microbiology, virology, recombinant DNA technology, peptide synthesis in solution, solid phase peptide synthesis, and immunology are described, for example, in the following texts that are incorporated by reference:

    • Sambrook, Fritsch & Maniatis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor. Laboratories, New York, Second Edition (1989), whole of Vols I, II, and III;
    • DNA Cloning: A Practical Approach, Vols. I and II (D. N. Glover, ed., 1985), IRL Press, Oxford, whole of text;
    • Oligonucleotide Synthesis: A Practical Approach (M. J. Gait, ed., 1984) IRL Press, Oxford, whole of text, and particularly the papers therein by Gait, pp 1-22; Atkinson et al., pp 35-81; Sproat et al., pp 83-115; and Wu et al., pp 135-151;
    • Animal Cell Culture: Practical Approach, Third Edition (John R. W. Masters, ed., 2000), ISBN 0199637970, whole of text;
    • Perbal, B., A Practical Guide to Molecular Cloning (1984);
    • Methods In Enzymology (S. Colowick and N. Kaplan, eds., Academic Press, Inc.), whole of series;
    • J. F. Ramalho Ortigäo, “The Chemistry of Peptide Synthesis” In: Knowledge database of Access to Virtual Laboratory website (Interactiva, Germany);
    • Barany, G. and Merrifield, R. B. (1979) in The Peptides (Gross, E. and Meienhofer, J. eds.), vol. 2, pp. 1-284, Academic Press, New York.
    • Bodanszky, M. (1984) Principles of Peptide Synthesis, Springer-Verlag, Heidelberg.
    • Bodanszky, M. & Bodanszky, A. (1984) The Practice of Peptide Synthesis, Springer-Verlag, Heidelberg.
    • Bodanszky, M. (1985) Int. J. Peptide Protein Res. 25, 449-474.
    • Golemis (2002) Protein-Protein Interactions: A Molecular Cloning Manual (Illustrated), Cold Spring Harbor Laboratory, New York, ISBN 0879696281.
    • Smith et al., (2002) Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology, 5th Edition (Illustrated), John Wiley & Sons Inc., ISBN 0471250929.
    • Sambrook and Russell (2001) Molecular Cloning, Cold Spring Harbor Laboratory, New York, ISBN 0879695773.


SUMMARY OF THE INVENTION
1. Introduction

The present invention provides peptidyl and non-peptidyl compositions that bind to CD40L (CD154) and/or prevent, reduce or inhibit CD40L from interacting with CD40 and/or modulate CD40 signaling and/or modulate CD40L signalling. The invention also relates to the use of such compositions in medicine. In one example, a composition of the present invention is for use in a method of diagnosis and/or prognosis and/or prophylaxis and/or therapy of the human or animal body. In another example, a composition of the present invention is for use or in an ex vivo method of diagnosis and/or prognosis and/or prophylaxis and/or therapy of the human or animal body. In another example, a composition of the present invention is for use in an in vivo or ex vivo method, such as a method of diagnosis and/or prognosis and/or prophylaxis and/or therapy, to ameliorate one or more adverse effects or consequences of CD40L signaling and/or CD40 signaling.


The present invention is based in part upon the identification by the inventors of compositions e.g., peptides and derivatives and analogs thereof that bind to CD40L e.g., at IC50 less than about 500 nM, and/or competitively antagonize or inhibit the interaction between CD40L and its cognate receptor CD40, and/or selectively inhibit or reduce CD40L-mediated expression of CD86 on primary B-cells and/or selectively antagonize or inhibit CD40L-mediated T-cell proliferation.


The peptides of the present invention are expressed from fragments of prokaryotic and compact eukaryotic genomes, not all of which are native open reading frames of those genomes. The peptides of the present invention are also not aptamers or peptide fragments derived from the native CD40-CD40L interface. For example, the peptides may have no known function or be derived from proteins having functions distinct from CD40 or CD40L. Accordingly, the peptides of the invention do not modulate CD40L-mediated events e.g., when expressed in their native contexts i.e., in the proteins in which they are expressed in nature. Alternatively, or in addition, the peptides of the present invention do not comprise N-terminal and C-terminal flanking cysteine residues, e.g., for conformational stability, as distinct from peptide aptamers. For example, the peptides of the present invention have lengths sufficient to form a secondary structure or super-secondary structure e.g. autonomously, such as without the need for flanking N-terminal and C-terminal cysteines to achieve their cyclization.


As used herein, the term “CD40L antagonist”, “CD40L peptidyl inhibitor” or “CD40L peptide inhibitor” or “CD40L inhibitor” or similar term shall be taken to mean a composition of the invention that binds to CD40L and inhibits or reduces or delays one or more CD40L-dependent effects in vitro or in vivo, e.g., a peptidyl inhibitor that binds to CD40L and inhibits one or more CD40L-mediated effects such as CD40L-mediated signaling, including CD40-CD40L costimulatory effects such as downstream effect(s) of CD40L-dependent CD40-mediated signaling.


The present invention is also based on the inventors' understanding that, in general, the serum half-life of a peptide of less than about 50-100 amino acids in length may be short, and that such a peptide may have lower affinity than desirable for pharmaceutical applications. The inventors reasoned that the half-life and/or the affinity of binding between a small peptide and its target may be enhanced inter alia by producing peptide derivatives and analogs such as: (i) a derivative having enhance entropy (e.g., PEGylated and/or HESylated and/or polyglycinated and/or multimeric forms of one or more base peptides having a desired activity); and/or (ii) a derivative comprising a “serum protein moiety” e.g., albumin or ferritin or transferrin or immunoglobulin or immunoglobulin fragment e.g., domain antibody (dAb) or modified Fc component of immunoglobulin lacking effector function or Fc-disable immunoglobulin such as a CovXBody; and/or (iii) a derivative comprising a “serum protein-binding moiety” e.g., albumin-binding peptide, albumin-binding domain (ABD or Affybody) or serum albumin-binding antibody domain (AlbudAb) that binds to albumin or immunoglobulin (Ig) or Ig fragment such as Fc; and/or (iv) an analog comprising D-amino acids e.g., a retro-peptide analog or retro-inverso analog of one or more base peptides and/or derivatives according to any example hereof and having a desired activity. For example, a derivatives or analog of a CD40L peptide inhibitor may have enhanced CD40L inhibitory activity and/or serum half-life compared to a corresponding base peptide from which it has been derived.


One example of the present invention provides a peptidyl inhibitor of the present invention e.g., a peptidyl inhibitor of an interaction between CD40 and CD40L and/or a peptidyl inhibitor that binds CD40L and/or a peptidyl inhibitor of one or more CD40-CD40L costimulatory effects, wherein the peptidyl inhibitor is encoded by a fragment of bacterial genome, and wherein the peptidyl inhibitor comprises a secondary structure or assembly of secondary structures that is identifiable, determinable or predictable from an amino acid sequence that is conserved with a different peptidyl inhibitor that binds independently to CD40L and which is encoded by a different genome fragment from the same organism.


The term “conserved” as used herein shall be understood to mean that a region of amino acid sequence present in two or more peptidyl inhibitors of the invention shows a relatively high level of sequence identity. The term “conserved” also encompasses nucleic acid sequence that encode a region of amino acid sequence present in two or more peptidyl inhibitors of the invention which shows relatively high level of sequence identity. The term “binds independently” as used herein shall be understood to mean that binding of one or more peptidyl inhibitors of the invention to CD40L is not dependent on binding of another peptidyl inhibitor of the invention to CD40L, or any other interaction or factor.


As used herein, the term “genome fragment” is intended to mean any isolated nucleic acid molecule having a sequence that is substantially identical to a portion of a chromosome of an organism e.g., a virus, prokaryotic organism, or eukaryotic organism. The term “genome fragment” may encompass DNA, RNA or an analog thereof.


For example, the peptidyl inhibitor may comprise a sequence that is conserved with a sequence of a different peptidyl inhibitor, wherein the peptidyl inhibitors each comprise different fragments of glycyl-tRNA synthetase from B. pertussis or different fragments of glycogen debranching enzyme from R. sphaeroides or different fragments of ABC peptide transporter from R. sphaeroides or different fragments of iron-sulfur protein from B. pertussis or different fragments of aliphatic amidase from Rhodopseudomonas palustris. Other such homologies are apparent from the data presented in Table 10.


As used herein, the term “homologies” shall be taken to mean one or more peptidyl inhibitor sequences which have been aligned and determined to share a region of conserved or substantially homologous amino acid sequence.


Another example of the present invention provides a peptidyl inhibitor of the present invention e.g., a peptidyl inhibitor of an interaction between CD40 and CD40L and/or a peptidyl inhibitor that binds CD40L and/or a peptidyl inhibitor of one or more CD40-CD40L costimulatory effects, wherein the peptidyl inhibitor comprises is encoded by a fragment of a bacterial genome, wherein the peptidyl inhibitor comprises a sequence that is conserved with a different peptidyl inhibitor that binds independently to CD40L and is encoded by an open reading frame from a different bacterium that is predicted to encode the same functional protein. For example, the peptidyl inhibitor may comprise a sequence that is conserved with a sequence of a different peptidyl inhibitor, wherein the peptidyl inhibitors are each predicted to be from glycyl-tRNA synthetases, such as the glycyl-tRNA synthetases from B. pertussis, R. sphaeroides and D. vulgaris. As used herein, the term “predicted to be from” shall be taken to mean that the amine acid sequence(s) being investigated, studied or tested for being a peptidyl inhibitor of CD40L is, based on data available e.g., sequence alignments, homology modeling and/or structural modeling, determined to be derived from the amino acid sequence of a particular gene and/or organism. Alternatively, the peptidyl inhibitor may comprise a sequence that is conserved with a sequence of a different peptidyl inhibitor, wherein the peptidyl inhibitors each predicted to be from ABC transporters, such as the ABC transporters from B. pertussis and P. aeruginosa. Alternatively, the peptidyl inhibitor may comprise a sequence that is conserved with a sequence of a different peptidyl inhibitor, wherein the peptidyl inhibitors each predicted to be from 3-hydroxydecanoyl-(acyl carrier protein) dehydratases, such as the 3-hydroxydecanoyl-(acyl carrier protein) dehydratases from R. sphaeroides and C. crescentus. Other such homologies are apparent from the data presented in Table 10.


In a further example, the present invention provides a peptidyl inhibitor of the present invention e.g., a peptidyl inhibitor of an interaction between CD40 and CD40L and/or a peptidyl inhibitor that binds CD40L and/or a peptidyl inhibitor of one or more CD40-CD40L costimulatory effects, wherein the peptidyl inhibitor comprises is encoded by a fragment of a bacterial genome, wherein the peptidyl inhibitor comprises a sequence that is conserved with a different peptidyl inhibitor that binds independently to CD40L and is encoded by a different genome fragment from the same organism and/or comprises a sequence that is conserved with a different peptidyl inhibitor that binds independently to CD40L and is encoded by an open reading frame from a different bacterium that is predicted to encode the same functional protein, and wherein the conserved sequences of the peptidyl inhibitors are predicted to assume a secondary structure or assembly of secondary structures in their conserved region e.g., the region of overlap between the amino acid sequences of different clones. As used herein, the term “different bacterium” shall be taken to mean two or more bacterial entities that are taxonomically distinct from one another. As such, it shall also be understood that peptidyl inhibitors of the invention may comprise amino acid sequence which is determined to be from the same functional protein, but which is encoded by nucleic acid sequence derived from the genome of taxonomically distinct bacterium. For example, the conserved sequences of the peptidyl inhibitors that align to glycyl tRNA synthetases, e.g., the glycyl tRNA synthetases of Thermotoga maritime, Desulfovibrio vulgaris, Rhodobacter sphaeroides and Bordetella pertussis, are predicted to assume an anti-parallel B sheet structure. As used herein, the term “conserved sequences” shall be taken to broadly mean any two or more amino acid sequences which comprise one or more regions of primary amino acid sequence which are substantially similar or identical to one another, and/or which form secondary protein structures which are substantially similar or identical to one another. The term “conserved sequences” may also encompass any two or more nucleic acid molecules e.g., DNA or RNA, which encode amino acid sequence comprising the one or more regions of primary amino acid sequence which are substantially similar or identical, and/or which form secondary protein structures which are substantially similar or identical. In another example, the conserved sequences of the peptidyl inhibitors that align to glycogen debranching enzymes, such as the glycogen debranching enzyme (GlgX) of Rhodobacter sphaeroides are predicted to assume an anti-parallel B sheet structure. In yet another example, conserved sequences with the peptidyl inhibitor M07 40L 0103 0859 aligns to a secondary structural region of a Salmonella enterica protein predicted to assume an alpha helix. Alternatively, or in addition to the preceding example, the present invention provides a peptidyl inhibitor of the present invention e.g., a peptidyl, inhibitor of an interaction between CD40 and CD40L and/or a peptidyl inhibitor that binds CD40L and/or a peptidyl inhibitor of one or more CD40-CD40L costimulatory effects, wherein the peptidyl inhibitor comprises is encoded by a fragment of a bacterial genome, wherein the peptidyl inhibitor comprises a sequence that is conserved with a different peptidyl inhibitor that binds independently to CD40L and is encoded by a different genome fragment from the same organism and/or comprises a sequence that is conserved with a different peptidyl inhibitor that binds independently to CD40L and is encoded by an open reading frame from a different bacterium that is predicted to encode the same functional protein, and wherein the conserved sequences of the peptidyl inhibitors are also present in one or more known secondary structures resolved by crystal structure determination. For example, sequences that are conserved with the peptidyl inhibitor M08 40L 0103 0716, including the peptidyl inhibitor M08 40L 0103 0716, is present in the anti-parallel beta sheet of a ferric alcaligin siderophore receptor resolved by crystal structure determination. In another example, sequences that are conserved with the peptidyl inhibitor M08 40L 0103 0755, including the peptidyl inhibitor M08 40L 0103 0755, is present in the anti-parallel beta sheet of benzoate 1,2-dioxygenase beta subunit polypeptide resolved by crystal structure determination.


The present invention also provides a peptidyl inhibitor of the present invention e.g., a peptidyl inhibitor of an interaction between CD40 and CD40L and/or a peptidyl inhibitor that binds CD40L and/or a peptidyl inhibitor of one or more CD40-CD40L costimulatory effects, wherein the peptidyl inhibitor comprises is encoded by a fragment of a bacterial genome, wherein the peptidyl inhibitor comprises a sequence that is conserved with a different peptidyl inhibitor that binds independently to CD4OL and is encoded by a different genome fragment from the same organism and/or comprises a sequence that is conserved with a different peptidyl inhibitor that binds independently to CD4OL and is encoded by an open reading frame from a different bacterium that is predicted to encode the same functional protein, and wherein the peptidyl inhibitors form conserved secondary structures or assemblies of secondary structures present in correctly-folded proteins.


As used herein, the term “functional protein” shall be understood to mean a protein which comprises one or more biological activities or functions. As used herein, the term “conserved secondary structure or assemblies of secondary structures” is intended to mean that peptidyl inhibitors of the invention may comprise amino acid sequence that, following folding of the amino acid chain, and in the case of protein assemblies, following an assembly of two or more secondary structures into a peptide or protein assembly, results in substantially similar or identical secondary structure and/or assemblies of secondary structures. See e.g., Table 1 for a list of protein secondary structures. In addition, the term “correctly folded proteins” as used herein shall be intended to mean that peptide inhibitors of the invention form the correct three-dimensional structures which are essential to the functionality of the native peptide i.e., the peptide inhibitors form conserved secondary structure or assemblies of secondary structures which do not comprise misfolds of the amino acid sequence.


As exemplified herein by way of Tables 8-10 and FIGS. 8 through 11, the inventors have demonstrated the general principal of the invention that peptidyl inhibitors of the invention form conserved secondary structures and/or assemblies of secondary structures e.g., that contribute to or are responsible for binding and/or inhibitory activity.


The data presented in Tables 8-10 and FIGS. 8 through 11 also indicate that it is possible to identify or determine or predict a secondary structure of a peptidyl inhibitor of the invention by performing a process comprising aligning primary sequence(s) of one or more peptidyl inhibitors having a predetermined activity to the primary sequence(s) of one or more known proteins or fragment(s) thereof, determining a secondary structure for the known protein(s) or fragment(s), and assigning the secondary structure for the known protein(s) or fragment(s) to the one or more peptidyl inhibitors. In one example, the process is performed in silico. As used herein, the term “predetermined activity” is intended to mean that the peptide inhibitor of the invention comprises a biological activity and/or biological function which has already been determined and/or which was previously known e.g., inhibition of an interaction between CD40 and CD40L and/or binding to CD40L and/or inhibition of one or more CD40-CD40L costimulatory effects. In another example, the process comprises interrogating a secondary structure database e.g., the PDB structural database, with primary sequence data to thereby identify or resolve secondary structures and assemblies of secondary structures in silico. In another example, the process comprises interrogating a protein crystal structure database with primary sequence data to thereby identify or resolve secondary structures and assemblies of secondary structures in silico. In another example, the process further comprises performing homology modelling and/or modelling of peptide docking on or binding to a target protein, e.g., based on the secondary structure predictions. In another example, the process further comprises performing rational drug design e.g., of small molecules based on the secondary structure predictions.


In one example, the present invention provides a composition comprising one or more peptides, analogs or derivatives, wherein a peptide, analog or derivative of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, and wherein said peptide, analog or derivative comprises a secondary structure or assembly of secondary structures of a protein, or a portion thereof, comprising an amino acid sequence that is substantially homologous and/or aligns to a consensus domain comprised in two or more amino acid sequences set forth in Table 10. As used herein, the term “comprises a secondary structure or assembly of secondary structures” shall be understood to mean that the subject peptide, analog or derivative is capable of forming a protein secondary structure. As used herein, the term “consensus domain”, “amino acid consensus domain” or similar, shall be understood to mean a region of primary amino acid sequence which is conserved or substantially conserved across two or more amino acid sequences of the invention, and which shows substantial homology within the consensus domain when the two or more sequence are aligned.


The peptide, analog or derivative may thus form a secondary structure or assembly of secondary structures comprised in a protein, or a portion thereof, selected from the group consisting of: glycyl-tRNA synthetase; glycogen debranching enzyme; ABC peptide transporter; iron-sulfur protein; aliphatic amidase; ribulokinase; extracellular solute binding protein; ABC transporter; rrnB0067; 3-hydroxydecanolyl-(acyl carrier protein) dehydratase; bifunctional GMP synthase/glutamine amidotransferase protein; acyl-coenzyme A synthetase; monooxygenase flavin-binding protein; DNA topoisomerase IV subunit B; acyl-coenzyme A synthetase; type IV secretion system protein; ATP-dependent helicase; alpha subunit of a dioxygenase; alpha amylase; a bacterial outer membrane protein (OMP); haemagglutinin, terminase large subunit; modification methylase; transposase; RSP2990; SAV4481; DRA0144; GSU1508; pNG7041; SAV2940; PA2G00938; SAV5325; CT1305; TGME49103250; Hlac3130; pNG6140; CC2361; PH1675; and Gifsy-1 prophage protein. The protein may preferably be a bacterial protein.


As used herein, the term “comprised in” in the context of a secondary structure or assembly of secondary structures shall be understood to mean that the subject secondary structure or assembly of secondary structures e.g., an anti-parallel beta, is derived from, and/or forms part of, a one or more proteins or enzymes, or a portion of one or more proteins or enzymes.


The glycyl-tRNA synthetase may be a bacterial glycyl tRNA syntetase, and is preferably a Rhodobacter sphaeroides glycyl-tRNA synthetase, a Bordetella pertussis glycyl-tRNA synthetase, or a Desulfovibrio vulgaris glycyl-tRNA synthetase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a glycyl-tRNA synthetase, as follows: VEDNWESPTLGAWGVGWEVWL(D/N)GME(I/V)(T/S)QFTYFQQ(I/V)GGX(D/S) (SEQ ID NO: 331). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 139; SEQ ID NO:156; SEQ ID NO:157; SEQ ID NO:159; SEQ ID NO:160; and SEQ ID NO: 161.


The glycogen debranching enzyme may be a Rhodobacter sphaeroides glycogen debranching enzyme. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a glycogen debranching enzyme comprising a primary sequence:











(SEQ ID NO: 332)



PLFSENATRVELcLFDETGQTQTHcLDLPSYEGGIWYGYL.






Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO:147; SEQ ID NO:148; and SEQ ID NO:167.


The ABC peptide transporter may be a, Rhodobacter sphaeroides ABC peptide transporter. For example, the peptide, analog or derivative may comprise a primary sequence formed by an alignment with an ABC peptide transporter comprising a primary sequence: MTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNM (SEQ ID NO: 333). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 58 and SEQ ID NO: 162.


The iron-sulfur protein may be a bacterial iron-sulfur protein, and may preferably be a Bordetella pertussis iron-sulfur protein or a Haloarcula marismortui iron-sulfur protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with an iron-sulfur protein comprising a primary sequence: LFRRPEFDFS (SEQ ID NO: 334); and/or NWKTF (SEQ ID NO: 335). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 9; SEQ ID NO: 84; SEQ ID NO: 158 and SEQ ID NO: 168.


The aliphatic amidase may be a Rhodopseudomonas palustris aliphatic amidase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with an aliphatic amidase comprising a primary sequence: GVFYYFGEGTV (SEQ ID NO: 336). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 149 and SEQ ID NO: 154.


The ribulokinase may be a Salmonella enterica ribulokinase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a ribulokinase comprising a primary sequence:











(SEQ ID NO: 337)



LWHESWGGLPPASFFDELDPCINRHLRYPLFSETFTADL.






Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 105 and SEQ ID NO: 155.


The extracellular solute binding protein may be a Rhodobacter sphaeroides extracellular solute binding protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with an extracellular solute binding protein comprising a primary sequence: MTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNM (SEQ ID NO: 338). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of SEQ ID NO: 58 and SEQ ID NO: 162.


The ABC transporter may be a Bordetella pertussis ABC transporter or a Pseudomonas aeruginosa ABC transporter. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with an ABC transporter comprising a primary sequence: PDMLLLDEPTNHLDA(E/D)SV(E/A)WLE(Q/H)FLH(K/D)FPGTVVA(V/I)THDRY FLDN(AN)A(E/G)WILELDRG(Y/H)GIP (SEQ ID NO: 339). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 61; SEQ ID NO: 71; SEQ ID NO: 108 and SEQ ID NO: 142.


The rrnB0067 protein may be a Haloarcula marismortui rrnB0067 protein or a Pseudomonas aeruginosa rrnB0067 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a rrnB0067 protein comprising a primary sequence: L(F/L)DHFRFCLTEFDRFDFSDHHGYLERNDWTIHDF(AN)GNGATGQFAVELT PDIIEETY (SEQ ID NO: 340). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 85 and SEQ ID NO: 171.


The 3-hydroxydecanolyl-(acyl carrier protein) dehydratase may be a bacterial 3-hydroxydecanolyl-(acyl carrier protein) dehydratase, and may preferably be a Rhodobacter sphaeroides hydroxydecanolyl-(acyl carrier protein) dehydratase or a Caulobacter crescentus hydroxydecanolyl-(acyl carrier protein) dehydratase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a 3-hydroxydecanolyl-(acyl carrier protein) dehydratase comprising a primary sequence: LM(M/F)DRI(T/V)(D/R)ISA(D/E)GG(L/K)(H/Y)GKG(H/Y)V(V/E)AEFDIHPDLWF F(E/D)CHF (SEQ ID NO: 341). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 118 and SEQ ID NO: 151.


The bifunctional GMP synthase/glutamine amidotransferase protein may be a Caulobacter crescentus bifunctional GMP synthase/glutamine amidotransferase protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a bifunctional GMP synthase/glutamine amidotransferase protein comprising a primary sequence: LIHEAIGDQLTcVFVDTGLLRKNEADQVVTLFRDHYNIPLVHVDAGDLFLGELA GVSDPET (SEQ ID NO: 342). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 152 and SEQ ID NO: 172.


The acyl-coenzyme A synthetase may be a Haloarcula marismortui acyl-coenzyme A synthetase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with an acyl-coenzyme A synthetase comprising a primary sequence: PEDRFFWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH (SEQ ID NO: 343). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 153 and SEQ ID NO: 163.


The monooxygenase flavin-binding protein may be a Caulobacter vibrioides (crescentus) monooxygenase flavin-binding protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of a monooxygenase flavin-binding protein comprising a primary sequence: LWTLQVTGPDGVETYTTNFLW(M/T)CQGYYRHSVGYTPEWPGMADFGGSIVH PQTWPADLD(L/R)K (SEQ ID NO: 344). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of SEQ ID NO: 173; SEQ ID NO: 174; SEQ ID NO: 175; SEQ ID NO: 176; SEQ ID NO: 177; SEQ ID NO: 178; SEQ ID NO: 179; SEQ ID NO: 180 and SEQ ID NO: 181.


The DNA topoisomerase IV subunit B may be a Streptomyces avermitilis DNA topoisomerase IV subunit B. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of a DNA topoisomerase IV subunit B comprising a primary sequence:











(SEQ ID NO: 345)



RLMHCLWEIIDNSVDEALGGYCDHIDVILHDDGSVEVRD.






Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 166 and SEQ ID NO: 182.


The acyl-coenzyme A synthetase may be a Haloarcula marismortui acyl-coenzyme A synthetase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of a acyl-coenzyme A synthetase comprising a primary sequence: FWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH (SEQ ID NO: 346). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 153; SEQ ID NO: 163; SEQ ID NO: 185 and SEQ ID NO: 186.


The type IV secretion system protein may be a Bordetella pertussis type IV secretion system protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of a type IV secretion system protein comprising a primary sequence: WWVFDNPNDcLDFSRPG(K/N)YGIDGTAFLDNAETRTPISMYLLHRM(N/S)EA MDGRRFVYLMDEAWKWIDDPAFAEFA (SEQ ID NO: 347). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 213; SEQ ID NO: 214; SEQ ID NO: 215; SEQ ID NO: 216; SEQ ID NO: 217; SEQ ID NO: 218; SEQ ID NO: 219; SEQ ID NO: 220; SEQ ID NO: 221; SEQ ID NO: 222; SEQ ID NO: 223; SEQ ID NO: 224 and SEQ ID NO: 225.


The ATP-dependent helicase may be a Streptomyces avermitilis ATP-dependent helicase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of an ATP-dependent helicase comprising a primary sequence: FKPKQLLGLTATPE(W/R)MDGLNVQD(K/E)FFEGRIAAELRLWEALENDLLCPF HYFGIPDGTDL (SEQ ID NO: 348). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 65; SEQ ID NO: 227 and SEQ ID NO: 228.


The dioxygenase may be a Haloarcula marismortui alpha subunit of a dioxygenase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of a dioxygenase comprising a primary sequence: L(C/G)EYEHAARYVSEVECNWKTFAGNYSECDHCHANHQDWITDIEL(A/E)E(S/P)ELEVNDYHWILH(C/Y)THDEDVEDEMRIHDEHEAKFYYFWPNF (SEQ ID NO; 349). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 93; SEQ ID NO: 100; SEQ ID NO: 114 and SEQ ID NO: 229.


The alpha amylase may be a Rhodobacter sphaeroides alpha amylase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of an alpha amylase comprising a primary sequence: LAYGKSTEDKQDFLLFHVNLDPHAAQT(F/L)EFEVP LW(E/G)FGLPDDASVEVE DLLNG(N/D)RFTWHGKWQWLELDPQT (SEQ ID NO: 350). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 116; SEQ ID NO: 230 and SEQ ID NO: 231.


The bacterial outer membrane protein (OMP) may be a Pseudomonas aeruginosa outer membrane protein (OMP). For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of a bacterial outer membrane protein (OMP) comprising a primary sequence: QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ (SEQ ID NO: 351). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 232; SEQ ID NO: 233; SEQ ID NO: 234; SEQ ID NO: 235; SEQ ID NO: 236; SEQ ID NO: 237; SEQ ID NO: 238 and SEQ ID NO: 239.


The haemagglutinin may be a Porphyromonas gingivalis haemagglutinin. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of a haemagglutinin comprising a primary sequence: RYYPLQVEYcVTAVYDESIESSTVCGTLHYATDAILYENFENGPVPNGWLVIDA DGDGFSWGHYLNAYDAFP(G/D)(H/Y)NR (SEQ ID NO: 352). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of SEQ ID NO: 240; SEQ ID NO: 241; SEQ ID NO: 242; SEQ ID NO: 243; SEQ ID NO: 244; SEQ ID NO: 245 and SEQ ID NO: 246.


The terminase large subunit may be a Rhodobacter sphaeroides terminase large subunit. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of a terminase large subunit comprising a primary sequence: LKEIADNANVQKVAFDRYKIKYFKRDMIDCGFDERWIDEHMVSYGQGF (SEQ ID NO: 353). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 247 and SEQ ID NO: 248.


The modification methylase may be a Bordetella pertussis modification methylase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of a modification methylase comprising a primary sequence: LDLFGDFNGLPEGADRTEFYQHEGHWQNRMILGDSLQVMASLAEREGLRGKV QOYFDPPYGIKFN (SEQ ID NO: 354). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 89 and SEQ ID NO: 101.


The transposase may be a bacterial transposase. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment of a transposase comprising a primary sequence: QVLRTLQVVTCRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN (SEQ ID NO: 355). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 303; SEQ ID NO: 304; SEQ ID NO: 305 and SEQ ID NO: 306.


The RSP2990 protein may be a Rhodobacter sphaeroides RSP2990 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by an alignment of a RSP2990 protein comprising a primary sequence:











(SEQ ID NO: 356)



RWYLGNQTAADDYLLESYGEHPQFPWTTQHIXK.






Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of SEQ ID NO: 128; SEQ ID NO: 187; SEQ ID NO: 188; SEQ ID NO: 189; SEQ ID NO: 190; SEQ ID NO: 191; SEQ ID NO: 192; SEQ ID NO: 193; SEQ ID NO: 194; SEQ ID NO: 195; SEQ ID NO: 196; SEQ ID NO: 197; SEQ ID NO: 198; SEQ ID NO: 199; SEQ ID NO: 200; SEQ ID NO: 201; SEQ ID NO: 202; SEQ ID NO: 203 and SEQ ID NO: 204.


The SAV4481 protein may be a Streptomyces avermitilis SAV4481 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a SAV4481 protein comprising primary sequence:











(SEQ ID NO: 357)



HNY(Y/C)WDDHYNSYYVVQYNHKYYWDYHYDCYYVVEK.






Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 113; SEQ ID NO: 115; SEQ ID NO: 121; SEQ ID NO: 130; SEQ ID NO: 132; SEQ ID NO: 135; SEQ ID NO: 138; SEQ ID NO: 143; SEQ ID NO: 257; SEQ ID NO: 258; SEQ ID NO: 259; SEQ ID NO: 260; SEQ ID NO: 261; SEQ ID NO: 262 and SEQ ID NO: 263.


The DR_A0144 protein may be a Deinococcus radiodurans DR_A0144 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a DR_A0144 protein comprising primary sequence: RDGNFDDTDRVGTVHDMRFVFLDNDTKLLFCTAYDDEWDPYIDDFATKIPDE LDLF (SEQ ID NO: 358). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 48 and SEQ ID NO: 79.


The GSU1508 protein may be a Geobacter sulfurreducens GSU1508 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a GSU1508 protein comprising primary sequence: R(L/I)PETRKAQAALATKYGIYGFCYYHYWFNGRRILESPVDAMLESGEPDFPF MLCWANENWT (SEQ ID NO: 359). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of SEQ ID NO: 49 and SEQ ID NO: 278.


The pNG7041 protein may be a Haloarcula marismortui pNG7041 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a pNG7041 protein comprising primary sequence:











(SEQ ID NO: 360)



LWNDGVSKEPFYEMDADLHLIDPNALIDWLGAWDQSDLT.






Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 54 and SEQ ID NO: 64.


The SAV2940 protein may be a Streptomyces avermitilis SAV2940 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a SAV2940 comprising primary sequence: L(L/Q)GEDMIEQLGRAHMSWGLGSADRWDLDQTTGIITWTFPDKTAAAPAQIL GSFSPGSGSWLWAWANK (SEQ ID NO: 361). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 66 and SEQ ID NO: 279.


The PA2G00938 protein may a Pseudomonas aeruginosa PA2G00938 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a PA2G00938 protein comprising primary sequence: LAEHAVWSLKCFPDWEWYNINIFGTDDPNHFWVECDGHGKILFPGYPEGYYE NHFLHSFELED (SEQ ID NO: 362). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 74 and SEQ ID NO: 280.


The SAV5325 protein may be a Streptomyces avermitilis SAV5325 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a SAV5325 protein comprising primary sequence: LGHIWASDVENAASFEPVDVGDEEAYKAGLLWLERLTMSDNGLRQLALFEW DEDGNLTKEWHAE (SEQ ID NO: 363). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 281 and SEQ ID NO: 282.


The CT1305 protein may be a Chlorobium tepidum CT1305 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a CT1305 protein comprising primary sequence: LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIR (SEQ ID NO: 364). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 137; SEQ ID NO: 284 and SEQ ID NO: 285.


The TGME49103250 protein may be a bacterial TGME49103250 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a TGME49103250 protein comprising primary sequence:











(SEQ ID NO: 365)



(G/R)(M/W)EWNGME(W/L)(N/K)(G/Q)XEW.






Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 109; SEQ ID NO: 287; SEQ ID NO: 288; SEQ ID NO: 289; SEQ ID NO: 290; SEQ ID NO: 291; SEQ ID NO: 292; SEQ ID NO: 293; SEQ ID NO: 294; SEQ ID NO: 295; SEQ ID NO: 296 and SEQ ID NO: 297.


The Hlac3130 protein may be a Haloarcula marismortui Hlac3130 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a Hlac3130 protein comprising primary sequence: LGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWCPVCGHEVFSHIPFEGVFC (SEQ ID NO: 366). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of SEQ ID NO: 308 and SEQ ID NO: 309.


The pNG6140 protein may be a Haloarcula marismortui pNG6140 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a pNG6140 protein comprising primary sequence: LGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWCPVCGHEVFSHIPFEGVFC (SEQ ID NO: 367). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of SEQ ID NO: 308 and SEQ ID NO: 309.


The CC2361 protein may be a Caulobacter crescentus CC2361 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a CC2361 protein protein comprising primary sequence: LYEYNADTPTSIYEAGVFQWLWLEDMIQQGALPEATDQFNSLHDQLAERFKAI FPN (SEQ ID NO: 368). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 315 and SEQ ID NO: 316.


The PH 1675 protein may be a Pyrococcus horikoshii PH 1675 protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a PH1675 protein comprising primary sequence: LVQGSKEATTIDESAELEALADAVAEEILSSDGIYFLGAGSTIKRIKDKIGIEGTL LGVDVVRVENGKAKLLVKDA (SEQ ID NO: 369). Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 327 and SEQ ID NO: 328.


The Gifsy-1 prophage protein may be a Salmonella enterica Gifsy-1 prophage protein. For example, the peptide, analog or derivative may comprise a primary sequence formed by alignment with a Gifsy-1 prophage protein comprising primary sequence:











(SEQ ID NO: 370)



LVCGWTDEDEIGLFVQVGAILRGESEITWGEPLYLSGVVT.






Alternatively, or in addition, the peptide, analog or derivative may comprise a primary sequence selected from the group consisting of: SEQ ID NO: 329 and SEQ ID NO: 330.


Alternatively, or in addition, the peptide, analog or derivative may form a secondary structure or assembly of secondary structures that is identifiable, determinable or predictable from any of the primary sequences herein described.


In another example, the present invention provides a composition comprising one or more peptides, analogs or derivatives, wherein a peptide, analog or derivative of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, and wherein said peptide, analog or derivative forms a secondary structure or assembly of secondary structures comprising an anti-parallel beta sheet. The anti-parallel beta sheet is the anti-parallel beta sheet comprised in a glycyl-tRNA synthetase, a glycogen debranching enzyme, a ferric alcaligin siderophore receptor, or a benzoate 1,2-dioxygenase beta subunit.


As used herein, the term “sequence of amino acids other than a sequence of CD40” shall be understood to mean any amino acid sequence which is not directly derived from the amino acid sequence of a CD40 gene or from a nucleic acid sequence encoding a CD40 gene or portion thereof.


For example, the anti-parallel beta sheet may be an anti-parallel beta sheet comprised in a glycyl-tRNA synthetase and be formed or formable from a primary sequence comprising: VEDNWESPTLGAWGVGWEVWL(D/N)GME(IN)(T/S)QFTYFQQ(IN)GGX(D/S) (SEQ ID NO: 331). Alternatively, or in addition, the anti-parallel beta sheet may be formed or formable from a primary sequence comprising a sequence selected from the group consisting of: SEQ ID NO: 139; SEQ ID NO:156; SEQ ID NO:157; SEQ ID NO:159; SEQ ID NO:160; and SEQ ID NO: 161.


In another example, the anti-parallel beta sheet is an anti-parallel beta sheet comprised in a glycogen debranching enzyme and is formed or formable from a primary sequence comprising:











(SEQ ID NO: 332)



PLFSENATRVELCLFDETGQTQTHCLDLPSYEGGIWYGYL.






Exemplary anti-parallel beta sheets in this group may be formed or formable from a primary sequence comprising a sequence selected from the group consisting of: SEQ ID NO: 147; SEQ ID NO:148; and SEQ ID NO:167.


In another example, the anti-parallel beta sheet may be an anti-parallel beta sheet comprised in a ferric alcaligin siderophore receptor, and be formed from a primary sequence comprising the sequence set forth in SEQ ID NO: 45.


In another example, the anti-parallel beta sheet may be an anti-parallel beta sheet comprised in a benzoate 1,2-dioxygenase beta subunit, formed or formable e.g., from a primary sequence comprising the sequence set forth in SEQ ID NO: 81.


In yet another example, the present invention provides a composition comprising one or more peptides, analogs or derivatives, wherein a peptide, analog or derivative of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, and wherein said peptide, analog or derivative forms a secondary structure or assembly of secondary structures comprising an alpha helix. For example, an alpha helix may be formed or formable from a primary sequence comprising the sequence set forth in SEQ ID NO: 126, SEQ ID NO: 139, SEQ ID NO: 156, SEQ ID NO: 159 or SEQ ID NO: 161.


In another example, the peptide, analog or derivative forms a secondary structure or assembly of secondary structures comprising an alpha helix, and further comprises an anti-parallel beta sheet e.g., a bacterial glycyl tRNA synthetase.


In another example, the present invention provides a composition comprising one or more peptides, analogs or derivatives, wherein a peptide, analog or derivative of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction of CD40 with CD40L, and/or one or more CD40-CD40L costimulatory effects, and wherein said peptide, analog or derivative comprises i.e., forms, a secondary structure or assembly of secondary structures which is identifiable, determinable or predictable from an amino acid sequence set forth in Table 8 or Table 10.


In yet another example, the present invention provides a composition comprising one or more peptides, analogs or derivatives, wherein a peptide, analog or derivative of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction, of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, and wherein said peptide, analog or derivative comprises a primary amino acid sequence set forth in Table 8 or a consensus domain amino acid sequence set forth in Table 10.


In each of the foregoing examples of the invention, the peptide, analog or derivative that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects may comprise a sequence encoded by a nucleic acid fragment of a prokaryote genome or a compact eukaryote genome e.g., wherein the peptide, analog or derivative comprises a sequence of a natural open reading frame of a prokaryote genome or a compact eukaryote genome. Preferred peptides do not comprise N-terminal and C-terminal cysteine residues necessary for achieving conformational stability, and are preferably cysteine-free. Alternatively, or in addition, the peptide, analog or derivative that binds CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects comprises one or more D amino acids, such as a retroinverso peptide analog. Alternatively, or in addition, the peptide, analog or derivative that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects is a peptidyl-fusion between a plurality of smaller peptides that each bind CD40L, wherein the peptidyl-fusion has a higher affinity for CD40L and/or enhanced inhibitory activity than a single peptide of the peptidyl-fusion, e.g., a dimer comprising two peptides that each bind CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects. Alternatively, or in addition, the peptide, analog or derivative that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects is a peptidyl-fusion between the peptide, analog or derivative that binds CD40L and a serum protein-binding moiety or serum protein moiety. Alternatively, or in addition, the peptide, analog or derivative that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L, and/or one or more CD40-CD40L costimulatory effects is a peptidyl-fusion between the peptide that binds CD40L and a protein transduction domain. Alternatively, or in addition, the peptide that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects comprises a polyethylene glycol (PEG) moiety, a hydroxyetheyl starch (HES) moiety, or a polyglycine moiety. Alternatively, or in addition, the composition of the invention may comprise a pharmaceutically acceptable carrier and/or excipient.


The composition of the invention is particularly useful for competitively antagonizing or inhibiting interaction between CD40L and CD40 and/or modulating CD40L-dependent signaling mediated by CD40L and/or CD40 and/or is suitable for use in a method of prophylaxis and/or therapy of one or more adverse effects or consequences of CD40L-dependent signaling mediated by CD40L and/or CD40 and/or for inhibiting or reducing expression of CD86 on B-cells and/or downstream signaling from CD86 and/or for antagonizing or inhibiting or reducing proliferation or differentiation of B-cells and/or antibody production by B-cells and/or for antagonizing or inhibiting or reducing proliferation or differentiation of T-cells and/or T-cell-mediated humoral immunity and/or in the prophylaxis or therapy of inflammation and/in the prophylaxis or therapy of an autoimmune disease and/in the attenuation or alleviation or amelioration of an inappropriate or adverse humoral immune response in a subject and/or in preventing or attenuating humoral immunity against one or more therapeutic proteins and/in the preparation of a medicament for antagonizing or inhibiting or reducing B-cell proliferation and/or antibody production and/or in the preparation of a medicament for antagonizing or inhibiting or reducing T-cell proliferation and/or in the preparation of a medicament for use in the prophylaxis or therapy of inflammation and/or in the preparation of a medicament for use in the prophylaxis or therapy of autoimmunity and/or in the preparation of a medicament for use in preventing or attenuating humoral immunity against one or more clotting factors in the treatment of hemophilia and/or in the preparation of a medicament for use in preventing or attenuating humoral immunity against one or more cytokines in the treatment of a viral infection and/or in the preparation of a medicament for use in preventing or attenuating humoral immunity against one or more cytokines in the treatment of a cancer or metastatic disease. Other uses in medicine are not excluded.


As used herein, a “therapeutic protein” or similar refers to any protein used in the therapy, treatment or prophylaxis of a disease, disorder or condition.


In a related example, the present invention provides a method of preventing or treating one or more adverse consequences of CD40L-dependent signaling in a subject, said method comprising administering an amount of the composition as described according to any example hereof for a time and under conditions sufficient to inhibit inappropriate CD40L-dependent signaling.


In a related example, the present invention provides a method of preventing or treating inflammation in a subject, said method comprising administering an amount of the composition as described according to any example hereof for a time and under conditions sufficient to ameliorate one or more adverse effects of CD40L-dependent signaling that contribute to an inflammatory response in a subject.


In a related example, the present invention provides a method of preventing or treating autoimmunity in a subject, said method comprising administering an amount of the composition as described according to any example hereof for a time and under conditions sufficient to ameliorate one or more adverse effects of CD40L-dependent signaling that contribute to autoimmunity in a subject.


In a related example, the present invention provides a method of preventing or treating cancer or metastatic disease in a subject, said method comprising administering an amount of the composition as described according to any example hereof for a time and under conditions sufficient to ameliorate one or more adverse effects of CD40L-dependent signaling that contribute to cancer in a subject.


In a related example, the present invention provides a method of treatment of a disease, or condition, said method comprising administering an amount of the composition as described according to any example hereof for a time and under conditions sufficient to attenuate or reduce humoral immunity against a therapeutic protein administered to the subject for treatment or prevention of the disease or condition. The method may further comprise administering the therapeutic protein to the subject.


In a related example, the present invention provides a method of treating a viral infection in a subject, said method comprising administering an amount of the composition as described according to any example hereof for a time and under conditions sufficient to attenuate or reduce humoral immunity against a cytokine administered to the subject.


In a related example, the present invention provides a method of treating hemophilia, said method comprising administering an amount of the composition as described according to any example hereof for a time and under conditions sufficient to attenuate or reduce humoral immunity against a clotting factor administered to the subject.


In yet another example, the present invention provides a method of identifying or determining or predicting a secondary structure of a peptidyl inhibitor of an interaction of CD40 with CD40L, wherein said method comprises aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit said interaction to the primary sequence(s) of one or more known proteins or fragment(s) thereof, determining a secondary structure for the known protein(s) or fragment(s), and assigning the secondary structure for the known protein(s) or fragment(s) to the one or more peptides; analogs or derivatives. The structure of known protein(s) or fragment(s) may be determined by reference to a database comprising a plurality of protein structures and/or comprising a plurality of structures of proteins which are paralogs, homologs and/or orthologs to the primary sequence(s). The primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction of CD40 with CD40L may be selected from the group set forth in Table 8 or 9 or 10 and/or the one or more known proteins may be selected from the homology groups set forth in Table 10 e.g., wherein the one or more known proteins are selected from the group consisting of glycyl-tRNA synthetase; glycogen debranching enzyme; ABC peptide transporter; iron-sulfur protein; aliphatic amidase; ribulokinase; extracellular solute binding protein; ABC transporter; rrnB0067; 3-hydroxydecanolyl-(acyl carrier protein) dehydratase; bifunctional GMP synthase/glutamine amidotransferase protein; acyl-coenzyme A synthetase; monooxygenase flavin-binding protein; DNA topoisomerase IV subunit B; acyl-coenzyme A synthetase; type IV secretion system protein; ATP-dependent helicase; alpha subunit of a dioxygenase; alpha amylase; a bacterial outer membrane protein (OMP); haemagglutinin; terminase large subunit; modification methylase; transposase; RSP2990; SAV4481; DR_A0144; GSU1508; pNG7041; SAV2940; PA2G00938; SAV5325; CT1305; TGME49103250; Hlac3130; pNG6140; CC2361; PH1675; and Gifsy-1 prophage protein.


For example, the known protein may be a glycyl-tRNA synthetase such as a Rhodobacter sphaeroides glycyl-tRNA synthetase, a Bordetella pertussis glycyl-tRNA synthetase, or a Desulfovibrio vulgaris glycyl-tRNA synthetase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a glycl-tRNA synthetase as follows:










(SEQ ID NO: 331)



VEDNWESPTLGAWGVGWEVWL(D/N)GME(I/V)(T/S)QFTYFQQ(I/V)GGX(D/S).







Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 139; SEQ ID NO:156; SEQ ID NO:157; SEQ ID NO:159; SEQ ID NO:160; and SEQ ID NO: 161.


In another example, the known protein may be a glycogen debranching enzyme e.g., a Rhodobacter sphaeroides glycogen debranching enzyme. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a glycogen debranching enzyme as follows:











(SEQ ID NO: 332)



PLFSENATRVELcLFDETGQTQTHcLDLPSYEGGIWYGYL.






Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO:147; SEQ ID NO:148; and SEQ ID NO:167.


In another example, the known protein may be a ABC peptide transporter e.g., a Rhodobacter sphaeroides ABC peptide transporter. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of an ABC peptide transporter as follows: MTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNM (SEQ ID NO: 333). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 58 and SEQ ID NO: 162.


In yet another example, the known protein may be an iron-sulfur protein, and may preferably be bacterial iron-sulfur protein e.g., a Bordetella pertussis iron-sulfur protein, or a Haloarcula marismortui iron-sulfur protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of an iron-sulfur protein, as follows: LFRRPEFDFS (SEQ ID NO: 334); and/or NWKTF (SEQ ID NO: 335). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 9; SEQ ID NO: 84; SEQ ID NO: 158 and SEQ ID NO: 168.


In another example, the known protein may be an aliphatic amidase e.g., a Rhodopseudomonas palustris aliphatic amidase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of an aliphatic amidase as follows: GVFYYFGEGTV (SEQ ID NO: 336). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 149 and SEQ ID NO: 154.


In another example, the known protein may be a ribulokinase e.g., a Salmonella enterica ribulokinase or, more specifically, a Salmonella enterica (typhimurium) ribulokinase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a ribulokinase as follows:











(SEQ ID NO: 337)



LWHESWGGLPPASFFDELDPCINRHLRYPLFSETFTADL.






Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary, sequence selected from the group consisting of SEQ ID NO: 105 and SEQ ID NO: 155.


In yet another example, the known protein may be an extracellular solute binding protein e.g., a Rhodobacter sphaeroides extracellular solute binding protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of an extracellular solute binding protein as follows:











(SEQ ID NO: 338)



TWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNM.






Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 58 and SEQ ID NO: 162.


In another example, the known protein may be an ABC transporter, and may preferably be a bacterial ABC transporter e.g., a Bordetella pertussis ABC transporter or a Pseudomonas aeruginosa ABC transporter. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of an ABC transporter as follows: PDMLLLDEPTNHLDA(E/D)SV(E/A)WLE(Q/H)FLH(K/D)FPGTVVA(V/I)THDRY FLDN(A/V)A(E/G)WILELDRG(Y/H)GIP (SEQ ID NO: 339). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 61; SEQ ID NO: 71; SEQ ID NO: 108 and SEQ ID NO: 142.


In another example, the known protein may be a rrnB0067 protein, and may preferably be a bacterial rrnB0067 protein e.g., a Haloarcula marismortui rrnB0067 protein or a Pseudomonas aeruginosa rrnB0067 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a rrnB0067 protein as follows: L(F/L)DHFRFCLTEFDRFDFSDHHGYLERNDWTIHDF(AN)GNGATGQFAVELT PDIIEETY (SEQ ID NO: 340). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 85 and SEQ ID NO: 171.


In yet another example, the known protein may be a 3-hydroxydecanolyl-(acyl carrier protein) dehydratase, and may preferably be a bacterial 3-hydroxydecanolyl-(acyl carrier protein) dehydratase e.g., a Rhodobacter sphaeroides hydroxydecanolyl-(acyl carrier protein) dehydratase or a Caulobacter crescentus hydroxydecanolyl-(acyl carrier protein) dehydratase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a 3-hydroxydecanolyl-(acyl carrier protein) dehydratase as follows: LM(M/F)DRI(TN)(D/R)ISA(D/E)GG(L/K)(H/Y)GKG(H/Y)V(V/E)AEFDIHPDLWF F(E/D)CHF (SEQ ID NO: 341). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 118 and SEQ ID NO: 151.


In another example, the known protein may be a bifunctional GMP synthase/glutamine amidotransferase protein e.g., a Caulobacter crescentus bifunctional GMP synthase/glutamine amidotransferase protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a bifunctional GMP synthase/glutamine amidotransferase protein as follows: LIHEAIGDQLTcVFVDTGLLRKNEADQVVTLFRDHYNIPLVHVDAGDLFLGELA GVSDPET (SEQ ID NO: 342). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 152 and SEQ ID NO: 172.


In another example, the known protein may be an acyl-coenzyme A synthetase e.g., a Haloarcula marismortui acyl-coenzyme A synthetase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of an acyl-coenzyme A synthetase as follows: PEDRFFWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH (SEQ ID NO: 343). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of SEQ ID NO: 153 and SEQ ID NO: 163.


In yet another example, the known protein may be a monooxygenase flavin-binding protein e.g., a Caulobacter vibrioides (crescentus) monooxygenase flavin-binding protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a monooxygenase flavin-binding protein as follows: LWTLQVTGPDGVETYTTNFLW(MMCQGYYRHSVGYTPEWPGMADFGGSIVH PQTWPADLD(L/R)K (SEQ ID NO: 344). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 173; SEQ ID NO: 174; SEQ ID NO: 175; SEQ ID NO: 176; SEQ ID NO: 177; SEQ ID NO: 178; SEQ ID NO: 179; SEQ ID NO: 180 and SEQ ID NO: 181.


In another example, the known protein may be a DNA topoisomerase IV subunit B e.g., a Streptomyces avermitilis DNA topoisomerase IV subunit B. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a DNA topoisomerase IV subunit B as follows:











(SEQ ID NO: 345)



RLMHcLWEIIDNSVDEALGGYcDHIDVILHDDGSVEVRD.






Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 166 and SEQ ID NO: 182.


In another example, the known protein may be an acyl-coenzyme A synthetase e.g., a Haloarcula marismortui acyl-coenzyme A synthetase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of an acyl-coenzyme A synthetase as follows: FWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH (SEQ ID NO: 346). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 153; SEQ ID NO: 163; SEQ ID NO: 185 and SEQ ID NO: 186.


In yet another example, the known protein may be a type IV secretion system protein e.g., a Bordetella pertussis type IV secretion system protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a type IV secretion system protein as follows: WWVFDNPNDcLDFSRPG(K/N)YGIDGTAFLDNAETRTPISMYLLHRM(N/S)EA MDGRRFVYLMDEAWKWIDDPAFAEFA (SEQ ID NO: 347). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 213; SEQ ID NO: 214; SEQ ID NO: 215; SEQ ID NO: 216; SEQ ID NO: 217; SEQ ID NO: 218; SEQ ID NO: 219; SEQ ID NO: 220; SEQ ID NO: 221; SEQ ID NO: 222; SEQ ID NO: 223; SEQ ID NO: 224 and SEQ ID NO: 225.


In another example, the known protein may be an ATP-dependent helicase e.g., a Streptomyces avermitilis ATP-dependent helicase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of an ATP-dependent helicase as follows: FKPKQLLGLTATPE(W/R)MDGLNVQD(K/E)FFEGRIAAELRLWEALENDLLCPF HYFGIPDGTDL (SEQ ID NO: 348). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of SEQ ID NO: 65; SEQ ID NO: 227 and SEQ ID NO: 228.


In another example, the known protein may be an alpha subunit of a dioxygenase e.g., a Haloarcula marismortui alpha subunit of a dioxygenase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of an alpha subunit of a dioxygenase as follows: L(C/G)EYEHAARYVSEVECNWKTFAGNYSECDHCHANHQDWITDIEL(A/E)E(S/P)ELEVNDYHWILH(C/Y)THDEDVEDEMRIHDEHEAKFYYFWPNF (SEQ ID NO: 349). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 93; SEQ ID NO: 100; SEQ ID NO: 114 and SEQ ID NO: 229.


In yet another example, the known protein may be an alpha amylase e.g., a Rhodobacter sphaeroides alpha amylase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of an alpha amylase as follows; LAYGKSTEDKQDFLLFHVNLDPHAAQT(F/L)EFEVPLW(E/G)FGLPDDASVEVE DLLNG(N/D)RFTWHGKWQWLELDPQT (SEQ ID NO: 350). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 116; SEQ ID NO: 230 and SEQ ID NO: 231.


In another example, the known protein may be a bacterial outer membrane protein (OMP) e.g., a Pseudomonas aeruginosa outer membrane protein (OMP). Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a bacterial outer membrane protein as follows: QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ (SEQ ID NO: 351). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 232; SEQ ID NO: 233; SEQ ID NO: 234; SEQ ID NO: 235; SEQ ID NO: 236; SEQ ID NO: 237; SEQ ID NO: 238 and SEQ ID NO: 239.


In another example, the known protein may be a haemagglutinin e.g., a Porphyromonas gingivalis haemagglutinin. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a haemagglutinin as follows: RYYPLQVEYcVTAVYDESIESSTVCGTLHYATDAILYENFENGPVPNGWLVIDA DGDGFSWGHYLNAYDAFP(G/D)(H/Y)NR (SEQ ID NO: 352). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 240; SEQ ID NO: 241; SEQ ID NO: 242; SEQ ID NO: 243; SEQ ID NO: 244; SEQ ID NO: 245 and SEQ ID NO: 246.


In another example, the known protein may be a terminase large subunit e.g., a Rhodobacter sphaeroides terminase large subunit. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a terminase large subunit as follows: LKEIADNANVQKVAFDRYKIKYFKRDMIDCGFDERWIDEHMVSYGQGF (SEQ ID NO: 353). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 247 and SEQ ID NO: 248.


In yet another example, the known protein may be a modification methylase e.g., a Bordetella pertussis modification methylase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a modification methylase as follows: LDLFGDFNGLPEGADRTEFYQHEGHWQNRMILGDSLQVMASLAEREGLRGKV QCIYFDPPYGIKFN (SEQ ID NO: 354). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 89 and SEQ ID NO: 101.


In another example, the known protein may be a transposase e.g., a bacterial transposase. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a transposase as follows: QVLRTLQVVTCRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN (SEQ ID NO: 355). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 303; SEQ ID NO: 304; SEQ ID NO: 305 and SEQ ID NO: 306.


In another example, the known protein may be a RSP2990 protein e.g., a Rhodobacter sphaeroides RSP2990 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a RSP2990 protein as follows:











(SEQ ID NO: 356)



RWYLGNQTAADDYLLESYGEHPQFPWTTQHIXK.






Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 128; SEQ ID NO: 187; SEQ ID NO: 188; SEQ ID NO: 189; SEQ ID NO: 190; SEQ ID NO: 191; SEQ ID NO: 192; SEQ ID NO: 193; SEQ ID NO: 194; SEQ ID NO: 195; SEQ ID NO: 196; SEQ ID NO: 197; SEQ ID NO: 198; SEQ ID NO: 199; SEQ ID NO: 200; SEQ ID NO: 201; SEQ ID NO: 202; SEQ ID NO: 203 and SEQ ID NO: 204.


In another example, the known protein may be a SAV4481 protein e.g., a Streptomyces avermitilis SAV4481 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a SAV4481 protein as follows:











(SEQ ID NO: 357)



HNY(Y/C)WDDHYNSYYVVQYNHKYYWDYHYDCYYVVEK.






Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of SEQ ID NO: 113; SEQ ID NO: 115; SEQ ID NO: 121; SEQ ID NO: 130; SEQ ID NO: 132; SEQ ID NO: 135; SEQ ID NO: 138; SEQ ID NO: 143; SEQ ID NO: 257; SEQ ID NO: 258; SEQ ID NO: 259; SEQ ID NO: 260; SEQ ID NO: 261; SEQ ID NO: 262 and SEQ ID NO: 263.


In yet another example, the known protein may be a DR_A0144 protein e.g., a Deinococcus radiodurans DR_A0144 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a DR_A0144 protein as follows: RDGNFDDTDRVGTVHDMRFVFLDNDTKLLFCTAYDDEWDPYIDDFATKIPDE LDLF (SEQ ID NO: 358). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 48 and SEQ ID NO: 79.


In another example, the known protein may be a GSU1508 protein e.g., a Geobacter sulfurreducens GSU1508 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a GSU1508 protein as follows: R(L/DPETRKAQAALATKYGIYGFCYYHWFNGRRILESPVDAMLESGEPDFPF MLCWANENWT (SEQ ID NO: 359). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 49 and SEQ ID NO: 278.


In another example, the known protein may be a pNG7041 protein e.g., a Haloarcula marismortui pNG7041 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a pNG7041 protein as follows:











(SEQ ID NO: 360)



LWNDGVSKEPFYEMDADLHLIDPNALIDWLGAWDQSDLT.






Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 54 and SEQ ID NO: 64.


In yet another example, the known protein may be a SAV2940 protein e.g., a Streptomyces avermitilis SAV2940 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives, that inhibit the interaction CD40 with CD40L to the primary sequence of a SAV2940 protein as follows: L(L/Q)GEDMIEQLGRAHMSWGLGSADRWDLDQTTGIITWTFPDKTAAAPAQIL GSFSPGSGSWLWAWANK (SEQ ID NO: 361). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 66 and SEQ ID NO: 279.


In another example, the known protein may be a PA2G00938 protein e.g., a Pseudomonas aeruginosa PA2G00938 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a PA2G00938 protein as follows: LAEHAVWSLKCFPDWEWYNINIFGTDDPNHFWVECDGHGKILFPGYPEGYYE NHFLHSFELED (SEQ ID NO: 362). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of SEQ ID NO: 74 and SEQ ID NO: 280.


In another example, the known protein may be a SAV5325 protein e.g., Streptomyces avermitilis SAV5325 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a SAV5325 protein as follows: LGHIWASDVENAASFEPVDVGDEEAYKAGLLWLERLTMSDNGLRQLALFEW DEDGNLTKEWHAE (SEQ ID NO: 363). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 281 and SEQ ID NO: 282.


In another example, the known protein may be a CT1305 protein e.g., a Chlorobium tepidum CT1305 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a CT1305 protein as follows: LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIR (SEQ ID NO: 364). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of SEQ ID NO: 137; SEQ ID NO: 284 and SEQ ID NO: 285.


In yet another example, the known protein may be a TGME49103250 protein e.g., a bacterial TGME49103250 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or, derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a TGME49103250 protein as follows:











(SEQ ID NO: 365)



(G/R)(M/W)EWNGME(W/L)(N/K)(G/Q)XEW.






Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 109; SEQ ID NO: 287; SEQ ID NO: 288; SEQ ID NO: 289; SEQ ID NO: 290; SEQ ID NO: 291; SEQ ID NO: 292; SEQ ID NO: 293; SEQ ID NO: 294; SEQ ID NO: 295; SEQ ID NO: 296 and SEQ ID NO: 297.


In another example, the known protein may be a Hlac3130 protein e.g., a Haloarcula marismortui Hlac3130 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a Hlac3130 protein as follows: LGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWCPVCGHEVFSHIPFEGVFC (SEQ ID NO: 366). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 308 and SEQ ID NO: 309.


In another example, the known protein may be a pNG6140 protein e.g., a Haloarcula marismortui pNG6140 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a pNG6140 protein as follows: LGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWCPVCGHEVFSHIPFEGVFC (SEQ ID NO: 367). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of SEQ ID NO: 308 and SEQ ID NO: 309.


In yet another example, the known protein may be a CC2361 protein e.g., a Caulobacter crescentus CC2361 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a CC2361 protein as follows: LYEYNADTPTSIYEAGVFQWLWLEDMIQQGALPEATDQFNSLHDQLAERFKAI FPN (SEQ ID NO: 368). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of SEQ ID NO: 315 and SEQ ID NO: 316.


In another example, the known protein may be a PH1675 protein e.g., a Pyrococcus horikoshii PH1675 protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a PH1675 protein as follows: LVQGSKEATTIDESAELEALADAVAEEILSSDGIYFLGAGSTIKRIKDKIGIEGTL LGVDVVRVENGKAKLLVKDA (SEQ ID NO: 369). Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 327 and SEQ ID NO: 328.


In another example; the known protein may be a Gifsy-1 prophage protein e.g., a Salmonella enterica Gifsy-1 prophage protein. Thus, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to the primary sequence of a Gifsy-1 prophage protein as follows:











(SEQ ID NO: 370)



LVCGWTDEDEIGLFVQVGAILRGESEITWGEPLYLSGVVT.






Alternatively, or in addition, the method may comprise aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit the interaction CD40 with CD40L to a primary sequence selected from the group consisting of: SEQ ID NO: 329 and SEQ ID NO: 330.


In yet another example, the present invention provides a method of identifying or determining or predicting a secondary structure of a peptidyl inhibitor of an interaction of CD40 with CD40L, wherein said method comprises aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit said interaction to the primary sequence(s) of one or more known proteins or fragment(s) thereof, determining a secondary structure for the known protein(s) or fragment(s), and assigning the secondary structure for the known protein(s) or fragment(s) to the one or more peptides, analogs or derivatives, wherein said peptide, analog or derivative forms a secondary structure or assembly of secondary structures comprising an anti-parallel beta sheet. The anti-parallel beta sheet may be an anti-parallel beta sheet comprised in a glycyl-tRNA synthetase, a glycogen debranching enzyme, a ferric alcaligin siderophore receptor, or a benzoate 1,2-dioxygenase beta subunit. For example, the anti-parallel beta sheet may be an anti-parallel beta sheet comprised in a glycyl-tRNA synthetase formed from a primary sequence comprising:










VEDNWESPTLGAWGVGWEVWL(D/N)GME(IN)(T/S)QFTYFQQ(1/V)GGX(D/S).







Alternatively, or in addition, the anti-parallel beta sheet may be formed from a primary sequence comprising a sequence selected from the group consisting of: SEQ ID NO: 139; SEQ ID NO:156; SEQ ID NO:157; SEQ ID NO:159; SEQ ID NO:160; and SEQ ID NO: 161.


In another example, the anti-parallel beta sheet is an anti-parallel beta sheet comprised in a glycogen debranching enzyme formed from a primary sequence comprising: PLFSENATRVELcLFDETGQTQTHcLDLPSYEGGIWYGYL. Alternatively, or in addition, the anti-parallel beta sheet may be formed from a primary sequence comprising a sequence selected from the group consisting of: SEQ ID NO: 147; SEQ ID NO:148; and SEQ ID NO:167.


In another example the anti-parallel beta sheet may be an anti-parallel beta sheet comprised in a ferric alcaligin siderophore receptor, e.g., formed from a primary sequence comprising the sequence set forth in SEQ ID NO: 45.


In another example the anti-parallel beta sheet may be an anti-parallel beta sheet comprised in a benzoate 1,2-dioxygenase beta subunit, e.g., formed from a primary sequence comprising the sequence set forth in SEQ ID NO: 81.


In yet another example, the present invention provides a method of identifying or determining or predicting a secondary structure of a peptidyl inhibitor of an interaction of CD40 with CD40L, wherein said method comprises aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit said interaction to the primary sequence(s) of one or more known proteins or fragment(s) thereof, determining a secondary structure for the known protein(s) or fragment(s), and assigning the secondary structure for the known protein(s) or fragment(s) to the one or more peptides, analogs or derivatives, wherein said peptide, analog or derivative forms a secondary structure or assembly of secondary structures comprising an alpha helix. For example, an alpha helix may be formed from a primary sequence comprising the sequence set forth in SEQ ID NO: 126.


In performing the method of the invention according to any example hereof, it is preferred that the peptide, analog or derivative that inhibits interaction of CD40 with CD40L does not comprise N-terminal and C-terminal cysteine residues for achieving conformational stability e.g., it is cysteine-free.


Alternatively, or in addition, the peptide, analog or derivative that inhibits interaction of CD40 with CD40L comprises one or more D amino acids e.g., it is a retroinverso peptide analog.


Alternatively, or in addition, the peptide, analog or derivative that inhibits interaction of CD40 with CD40L is a peptidyl-fusion between a plurality of smaller peptides that each bind CD40L, wherein the peptidyl-fusion has a higher affinity for CD40L and/or enhanced inhibitory activity than a single peptide of the peptidyl-fusion e.g., the peptidyl-fusion is a dimer comprising two peptides that each bind CD40L and partially or completely inhibits interaction of CD40 with CD40L.


Alternatively, or in addition, the peptide, analog or derivative that inhibits interaction of CD40 with CD40L is a peptidyl-fusion between the peptide, analog or derivative that binds CD40L and a serum protein-binding moiety or serum protein moiety.


Alternatively, or in addition, the peptide that inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects is a peptidyl-fusion between the peptide that binds CD40L and a protein transduction domain.


Alternatively, or in addition, the peptide that inhibits interaction of CD40 with CD40L comprises a polyethylene glycol (PEG) moiety, a hydroxyethyl starch (HES) moiety, or a polyglycine moiety.


In performing the method of identifying or determining or predicting a secondary structure of a peptidyl inhibitor, it is preferred to first isolate the peptide, analog or derivative that inhibits the pre-determined interaction e.g., an interaction of CD40 with CD40L, e.g., by performing an assay that measures the pre-determined activity and isolating the peptidyl inhibitor by virtue of it having the pre-determined activity. For example, the peptidyl inhibitor may be isolated from other peptides that do not have the pre-determined activity. This example applies mutatis mutandis to a process in which the peptidyl inhibitor is isolated and a secondary structure or assembly of secondary structures is identified or determined or predicted or aligned as described according to any example hereof.


Alternatively, or in addition, it is preferred to perform a process to permit the primary structure of the peptidyl inhibitor to be determined. For example, an isolated peptidyl inhibitor or nucleic acid encoding a peptidyl inhibitor may be sequenced to thereby determine the primary structure of the peptidyl inhibitor before identifying or determining or predicting a secondary structure of a peptidyl inhibitor.


In yet another example, the present invention provides a PEGylated peptidyl inhibitor of CD40L-dependent signaling. In another example, the present invention provides a HESylated peptidyl inhibitor of CD40L-dependent signaling. In another example, the present invention provides a polyglycinated peptidyl inhibitor of CD40L-dependent signaling. In another example, the present invention provides a composition comprising a peptidyl inhibitor of CD40L-dependent signalling as described according to any example hereof and a serum protein moiety as described according to any example hereof. In another example, the present invention provides a composition comprising a peptidyl inhibitor of CD40L-dependent signalling as described according to any example hereof and a peptidyl serum protein-binding moiety as described according to any example hereof. In another example, the present invention provides a composition comprising a peptidyl inhibitor of CD40L-dependent signalling as described according to any example hereof and a non-peptidyl serum protein-binding moiety as described according to any example hereof e.g., a hapten that binds to an Fc-disabled antibody, polyethylene glycol, hydroxyethyl starch (HES), polyglycine, a 4,4-diphenylcyclohexyl moiety or 4-phenylbutanoic acid moiety.


Another example of the present invention provides a PEGylated chiral analog of a peptidyl inhibitor of CD40L-dependent signalling as described according to any example hereof. In another example, the present invention provides a HESylated chiral analog of a peptidyl inhibitor of CD40L-dependent signaling as described according to any example hereof. In another example, the present invention provides a polyglycinated chiral analog of a peptidyl inhibitor of CD40L-dependent signaling as described according to any example hereof. In another example, the present invention provides a composition comprising a chiral analog of a peptidyl inhibitor of CD40L-dependent signalling as described according to any example hereof and a serum protein moiety as described according to any example hereof wherein the serum protein moiety may itself be a chiral analog such as by comprising D-amino acids, or it may comprise L-amino acids. In another example, the present invention provides a composition comprising a chiral analog of a peptidyl inhibitor of CD40L-dependent signalling as described according to any example hereof and a peptidyl serum protein-binding moiety as described according to any example hereof, wherein the serum protein-binding moiety may itself be a chiral analog such as by comprising D-amino acids, or it may comprise L-amino acids. In another example, the present invention provides a composition comprising a chiral analog of a peptidyl inhibitor of CD40L-dependent signalling as described according to any example hereof and a non-peptidyl serum protein-binding moiety e.g., a hapten that binds to Fc, polyethylene glycol, hydroxyethyl starch (HES), polyglycine, a 4,4-diphenylcyclohexyl moiety or 4-phenylbutanoic acid moiety e.g., conjugated to D-lysine.


The present invention also provides compositions comprising cysteine-free peptidyl inhibitors of CD40L-dependent signalling, wherein the peptidyl inhibitor moiety lacks cysteine residues e.g., by virtue of substitution of cysteine for another amino acid such as serine. Such compositions may be PEGylated, HESylated, polyglycinated, multimerized, or comprise serum protein moiety or serum protein-binding moiety with or without intervening spacer as described according to any example hereof, and they may be chiral analogs according to any example hereof e.g., retroinverted analogs.


The present invention also provides a multimeric peptidyl inhibitor of CD40L-dependent signaling. As used in this context, the term “multimeric peptidyl inhibitor” shall be taken to mean that the composition comprises two or more peptidyl inhibitors that each inhibit CD40L-dependent signalling in their monomeric form. In one example, homodimers and heterodimers have enhanced inhibitory activity compared to a monomeric peptide from which it is derived e.g., with respect to CD40L binding to CD40 and/or inhibition of CD40L binding to a cognate binding partner such as CD40 or Mac-1 and/or inhibition of one or more CD40L-dependent effects such as B-cell proliferation or T-cell proliferation. For example, the effect of multimerization is more than the additive effect of either base peptide. A multimeric peptidyl inhibitor of CD40L-dependent signaling may be PEGylated, HESylated, polyglycinated, multimerized, or comprise a serum protein moiety or a serum protein-binding moiety with or without intervening spacer as described according to any example hereof, and may be a chiral analog according to any example hereof e.g., a retroinverted analog.


More particularly, another example of the present invention provides a PEGylated peptidyl inhibitor of CD40L-dependent signalling or a PEGylated multimeric peptidyl inhibitor of CD40L-dependent signaling, wherein the peptidyl inhibitor lacks cysteine residues e.g., by virtue of substitution of cysteine for another amino acid such as serine, or comprises a single N-terminal or C-terminal cysteine residue. In yet another example, the present invention provides a PEGylated chiral analog of a peptidyl inhibitor of CD40L-dependent signaling, wherein the peptidyl inhibitors lack cysteine residues e.g., by virtue of substitution of cysteine for another amino acid such as serine.


The present invention also extends to the production and/or use of recombinant combinatorial proteins comprising pluralities of the peptides, derivatives and analogs described according to any example of the invention herein, e.g., recombinantly-produced peptidomimetics comprising one or more protein sub-domains, domains, folds, secondary structures or super-secondary structures.


The present invention also extends to pharmaceutical compositions comprising the synthetic and recombinant peptide-based i.e., “peptidyl” CD40L-binding compositions described according to any examples hereof, and to the use of such compositions in medicine and/or pharmacy. For example, the peptides and any analogs or derivatives thereof may be formulated with a suitable carrier or excipient e.g., for injection or inhalation or oral administration.


The present invention also extends to non-peptidyl equivalents of the exemplified peptides, peptidyl analogs and peptidyl derivatives provided herein, and to compositions comprising same and methods for their production and/or use in medicine and/or pharmacy. For example, the non-peptidyl equivalents may be formulated with a suitable carrier or excipient e.g., for injection or inhalation or oral administration.


The present invention also extends to diagnostic, prognostic, prophylactic, therapeutic and research applications of the peptides and compositions of the invention described herein.


2. General

As used herein the term “derived from” shall be taken to indicate that a specified integer may be obtained from a particular source albeit not necessarily directly from that source.


Throughout this specification, unless the context requires otherwise, the word “comprise”, or variations such as “comprises” or “comprising”, will be understood to imply the inclusion of a stated step or element or integer or group of steps or elements or integers but not the exclusion of any other step or element or integer or group of elements or integers.


Throughout this specification, unless specifically stated otherwise or the context requires otherwise, reference to a single step, composition of matter, group of steps or group of compositions of matter shall be taken to encompass one and a plurality (i.e. one or more) of those steps, compositions of matter, groups of steps or group of compositions of matter.


Each embodiment described herein is to be applied mutatis mutandis to each and every other embodiment unless specifically stated otherwise.


Those skilled in the art will appreciate that the invention described herein is susceptible to variations and modifications other than those specifically described. It is to be understood that the invention includes all such variations and modifications. The invention also includes all of the steps, features, compositions and compounds referred to or indicated in this specification, individually or collectively, and any and all combinations or any two or more of said steps or features.


The present invention is not to be limited in scope by the specific examples described herein, which are intended for the purpose of exemplification only. Functionally-equivalent products, compositions and methods are clearly within the scope of the invention, as described herein.


3. Specific Examples

The scope of the invention will be apparent from the claims as filed with the application, which are hereby incorporated into the description. The scope of the invention will also be apparent from the following specific examples.


One example of the present invention provides a composition comprising one or more peptides, wherein a peptide of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects. The peptide that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects is a peptidomimetic or other composition not derived from the sequence of CD40 or other binding partner of CD40L e.g., Mac-1, and does not comprise a sequence of CD40 or other binding partner. For example, the peptide is not derived from the native Cd40-Cd40L interface and does not comprise a sequence thereof. In one example, the peptide comprises a sequence encoded by a nucleic acid fragment of a prokaryote genome or a compact eukaryote genome e.g., the peptide comprises a sequence of a natural open reading frame of a prokaryote genome or a compact eukaryote genome.


In another example, the peptide that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects does not comprise both N-terminal and C-terminal cysteine residues for achieving conformational stability e.g., by virtue of disulfide bridge formation leading to cyclic peptide formation, which cyclic peptide formation may be advantageous for aptamer functionality. In another example, the peptide of the present invention comprises a single C-terminal or N-terminal cysteine residue. In another example, the peptide is cysteine-free.


In another example, the peptide that binds CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects comprises one or more non-naturally-occurring amino acids e.g., one or more D amino acids. For example, the peptide is an isostere or a chiral analog such as a retroinverso-peptide analog (i.e., a retro-inverted peptide).


In another example, the peptide that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects is a peptidyl-fusion. As used herein, the term “peptidyl fusion” means a peptide comprising two or more peptidyl moieties or sub-units linked covalently or non-covalently, with or without intervening linker or spacer moieties separating the peptidyl moieties. Preferred peptidyl fusions comprise two or more peptidyl moieties linked covalently e.g., by means of oxime chemistry or peptide synthesis or recombinant protein synthesis. For example, the peptidyl fusion may comprise a plurality of smaller peptides that each bind CD40L, wherein the peptidyl-fusion has a higher affinity for CD40L and/or enhanced inhibitory activity than a single peptide of the peptidyl-fusion. These smaller peptides that each bind CD40L may be the same or comprise the same sequence or secondary structure, or they may be structurally different e.g., with respect to their primary amino acid sequences or with respect to their secondary structures. For example, the peptidyl-fusion may be a dimer e.g., a homodimer or heterodimer comprising two peptides that each bind CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects. In another example, a peptidyl-fusion is between a peptide that binds CD40L and a serum protein-binding moiety, or between a peptide that binds CD40L and a protein transduction domain, or between a peptide that binds CD40L and a serum protein-binding moiety. Peptidyl fusions comprising any combination of one or more peptides of the invention that bind CD40L and one or more other moieties e.g., a serum protein-binding moiety and/or a protein transduction domain and/or a serum protein-binding moiety, are also encompassed by the invention.


As with base peptides, peptidyl fusions of the present invention may include one or more D-amino acids, and be isosteres or chiral analogs of peptides comprising L-amino acids.


Peptides, peptidyl fusions, isosteres and chiral analogs of the invention as described according to any example hereof may further comprise a polyethylene glycol (PEG) residue i.e., they may be PEGylated compositions. For example, the invention provides a peptide that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, wherein the peptide comprises a PEGylated peptide having L-amino acids or a PEGylated chiral analog thereof wherein all amino acids other than glycine have been substituted for D-amino acids. The PEGylated peptides and analogs may lack N-terminal and C-terminal cysteines or they may be cysteine-free.


In another example, the present invention provides a composition comprising a peptide that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, wherein the peptide comprises a sequence selected individually or collectively from the group consisting of:

  • (i) a sequence set forth in any one of SEQ ID NOs: 1 to 34 or 44, or SEQ ID Nos: 45 to 330, or SEQ ID Nos: 331 to 393;
  • (ii) the sequence of a functional fragment of any one of SEQ ID NOs: 1 to 34 or 44;
  • (iii) the sequence of a peptidyl fusion comprising a plurality of sequences at (i) or (ii), optionally wherein at least one of said plurality is separated by a spacer or linker molecule;
  • (iv) the sequence of (i) or (ii) or (iii) additionally comprising a protein transduction domain, e.g., a HIV tat basic region or a retroinverted analog thereof and/or a serum protein-binding moiety, optionally wherein said peptide is separated from the protein transduction domain and/or serum protein-binding moiety by a spacer or said protein transduction domain and/or serum protein-binding moiety are separated by one or more spacers; and
  • (v) an analog of any one of (i) to (iv) selected from the group consisting of (a) the sequence of any one of (i) to (iv) comprising one or more non-naturally-occurring amino acids; (b) the sequence of any one of (i) to (iv) comprising one or more non-naturally-occurring amino acid analogs; (c) an isostere of any one of (i) to (iv); (d) a retro-peptide analog of any one of (i) to (iv); and (e) a retro-inverted peptide analog of any one of (i) to (iv).


For the purposes of nomenclature, the sequences set forth in SEQ ID Nos: 1 to 44, or SEQ ID Nos: 45 to 330, or SEQ ID Nos: 331 to 393 are representative peptides of the present invention that bind to CD40L and inhibit the interaction of CD40 with a cognate receptor e.g., CD40, and inhibit downstream signaling from CD40L including costimulatory CD40-CD40L signaling e.g., B-cell proliferation such as determined by CD86 expression levels, and T-cell proliferation. Additional specific examples of such peptidyl inhibitors of the present invention are to be found e.g., in Australian Patent Application No. 2008903552 filed Jul. 11, 2009, the contents of which are incorporated herein in their entirety. It will also be apparent from Example 1 hereof that such peptidyl inhibitors include specific examples of cysteine-free peptides and peptidyl fusions, and retroinverted peptides, in both unmodified form and as PEGylated peptides and peptidyl fusions.


A further example of the present invention provides a phagemid vector or cell capable of expressing a peptide or peptidyl fusion of the present invention as described according to any example hereof comprising naturally-occurring amino acids or otherwise capable of being expressed by cellular translational machinery.


A still further example of the present invention provides an isolated nucleic acid comprising a sequence that encodes a peptide or peptidyl fusion of the present invention as described according to any example hereof.


In another example, the composition of the present invention is suitable for administration to a human or non-human animal. For example, the composition is formulated so as to comprise the active peptidyl agent and a pharmaceutically acceptable carrier and/or excipient.


In one example, the composition is a liquid pharmaceutical formulation comprising a buffer in an amount to maintain the pH of the formulation in a range of about pH 5.0 to about pH 7.0. In a further example, the pharmaceutical composition comprises an isotonizing agent in an amount to render same composition near isotonic. Exemplary isotonizing agents include sodium chloride e.g., present in said formulation at a concentration of about 50 mM to about 300 mM, or at a concentration of about 150 mM. Exemplary buffers are selected from the group consisting of succinate, citrate, and phosphate buffers e.g., at a concentration of about 1 mM to about 50 mM. For example, a sodium succinate or sodium citrate-buffer at a concentration of about 5 mM to about 15 mM may be employed. In another example, the formulation further comprises a surfactant in an amount from about 0.001% to about 1.0% e.g., polysorbate 80 which may be present in said formulation in an amount from about 0.001% to about 0.5%.


Pharmaceutical compositions may be formulated for administration by injection, inhalation, ingestion or topically.


In one example, the formulation is for inhalation and the subject peptide is present in an amount suitable for administration by inhalation and the carrier or excipient is one suitable for inhalation. Inhalable formulations e.g., comprising an alkyl-saccharide transmucosal delivery-enhancing excipient such as Intraveil (Aegis Therapeutics) are preferred for prophylactic applications e.g., for administration to an asymptomatic subject at risk of developing a condition associated with CD40L signaling or a complication associated therewith e.g., an asymptomatic subject having one or more risk factors for a condition associated with CD40L signaling supra, and/or an asymptomatic subject, exposed to an infectious agent, poison, allergen or irritant of the airways that is a risk factor for development of a condition associated with CD40L signaling.


By “asymptomatic subject” is meant a subject that does not exhibit one or more symptoms of a condition associated with CD40L signaling.


In another example, the formulation is for injection and the subject peptide is present in an amount suitable for administration by injection e.g., subcutaneously, intravenously, intraperitoneally or intramuscularly, and the carrier or excipient is one suitable for, injection e.g., subcutaneously, intravenously, intraperitoneally or intramuscularly. Injectable formulations are preferred for acute phase a condition associated with CD40L signaling or complications associated therewith or where the subject has difficulty inhaling.


It is to be understood that it is a preferred embodiment for the peptidyl formulations of the present invention to have CD40L signaling inhibitory activity conferred by the peptide component or peptide analog component of such formulations.


The formulation may be packaged for multiple administrations e.g., it may be packaged as multiple injectable ampoules, capsules, etc. for repeated administration or repeated dosing.


The CD40L signaling inhibitor may be a peptidyl or non-peptidyl composition. Suitable peptidyl compounds will be any one or more of the peptides described herein above, or alternatively a different peptide or antibody composition that inhibits CD40L signaling e.g., as described in the ensuing Detailed Description. Suitable non-peptidyl compounds will be apparent to the skilled artisan based on the description herein, and include, for example, a nucleic acid, or a small molecule.


Again, the present invention clearly encompasses formulations comprising mixtures of peptides or peptide analogs.


In one example, a peptide or analog as described herein above or a peptidyl CD40L signaling inhibitor, is conjugated to or fused to a protein transduction domain. A suitable protein transduction domain will be apparent to the skilled artisan based on the description herein and includes a HIV-tat basic region peptide or a retroinverted analog thereof. Another suitable protein transduction domain is a Kaposi fibroblast growth factor (FGF) hydrophobic peptide protein transduction domain or a retro-inverted analog thereof.


The skilled artisan will be aware that an amount of the active ingredient will vary, e.g., as a result of variation in the bioactivity of an inhibitor, and/or the severity of the condition being treated. Accordingly, the term “amount” is not to be construed to limit the invention to a specific quantity, e.g., weight of active ingredient.


As used herein, the term “suitable carrier or excipient” shall be taken to mean a compound or mixture thereof that is suitable for use in a formulation albeit not necessarily limited in use to that context. In contrast, the term “a carrier or excipient” is compound or mixture thereof that is described in the art only with reference to a use in a formulation. The term “carrier or excipient for inhalation” shall be taken to mean a compound or mixture thereof that is suitable for use in a formulation to be administered to a subject by inhalation e.g., a formulation comprising an alkyl-saccharide transmucosal delivery-enhancing excipient such as Intraveil (Aegis Therapeutics). The term “carrier or excipient for injection” shall be taken to mean a compound or mixture thereof that is suitable for use in a formulation to be administered to a subject by injection.


A carrier or excipient useful in the formulation of the present invention will generally not inhibit to any significant degree a relevant biological activity of the active compound e.g., the carrier or excipient will not significantly inhibit the activity of the active compound with respect to reducing neutrophilic inflammation. Alternatively, or in addition, the carrier or excipient comprises a compound that enhances uptake and/or delivery and/or efficacy of the active compound.


Alternatively, or in addition, the carrier or excipient comprises a compound that enhances the activity of a peptide or analog as described herein above or, more generally, an CD40L signaling inhibitor and/or reduces inhibition of said peptide or analog or CD40L signaling inhibitor by degradative enzymes in the site of administration and/or en route to the site of action of a subject and or at the site of action. For example, the carrier or excipient may comprise a protease inhibitor and/or a DNase inhibitor and/or an RNase inhibitor to thereby enhance the stability of a peptide or analog as described herein above or a peptidyl CD40L signaling inhibitor.


In one example, the formulation as described herein according to any embodiment comprises an additional compound, such as, for example, a corticosterioid to further enhance the efficacy of the peptide or analog e.g., in anti-inflammatory applications. Suitable additional compounds will be apparent to the skilled artisan based on the description herein.


The present invention also provides a method for producing a formulation described herein according to any embodiment. For example, such a method comprises mixing or otherwise combining a peptide or analog as described herein above or CD40L signaling inhibitor in an amount sufficient to reduce or prevent a CD40L-mediated signaling event with a suitable carrier or excipient e.g., a carrier or excipient for inhalation or injection. In one example, the method additionally comprises producing or obtaining said peptide or analog or CD40L signaling inhibitor. For example, a peptide or analog or CD40L signaling inhibitor is produced synthetically or recombinantly, using a method known in the art and/or described herein.


The composition of the invention is suitable for use in medicine e.g., in a method of treatment of the human or animal body by prophylaxis or therapy, or for use in research e.g., in a method of drug screening, drug development or clinical trial. For example, a composition of the invention according to any example hereof is for competitively antagonizing or inhibiting interaction between CD40L and CD40 in medicine and/or for research. In another example, a composition of the invention according to any example hereof is for modulating CD40L-dependent signaling mediated by CD40L and/or CD40, including costimulatory CD40-CD40L signaling. In another example, a composition of the invention according to any example hereof is for modulating CD40L-dependent signaling mediated by CD40L and/or Mac-1, including costimulatory CD40-Mac-1 signaling. In another example, a composition of the invention according to any example hereof is for use in a method of prophylaxis and/or therapy of one or more adverse effects or consequences of CD40L-dependent signaling mediated by CD40L and/or CD40 and/or Mac-1. In another example, a composition of the invention according to any example hereof is inhibiting or reducing expression of CD86 on B-cells and/or downstream signaling from CD86. In another example, a composition of the invention according to any example hereof is for antagonizing or inhibiting or reducing proliferation or differentiation of B-cells and/or antibody production by B-cells. In another example, a composition of the invention according to any example hereof is for antagonizing or inhibiting or reducing proliferation or differentiation of T-cells and/or T-cell-mediated humoral immunity. In another example, a composition of the invention according to any example hereof is for use in the prophylaxis or therapy of inflammation. In another example, a composition of the invention according to any example hereof is for use in the prophylaxis or therapy of an autoimmune disease. In another example, a composition of the invention according to any example hereof is for use in the attenuation or alleviation or amelioration of an inappropriate or adverse humoral immune response in a subject. In another example, a composition of the invention according to any example hereof is for use in preventing or attenuating humoral immunity against one or more therapeutic proteins e.g., clotting agent(s) and/or cytokine(s).


In related examples, the present invention provides for use of a composition of the invention, according to any example hereof in medicine and/or in the preparation of a medicament for antagonizing or inhibiting or reducing B-cell proliferation and/or antibody production and/or for antagonizing or inhibiting or reducing T-cell proliferation and/or for use in the prophylaxis or therapy of inflammation and/or for use in the prophylaxis or therapy of autoimmunity and/or for use in preventing or attenuating humoral immunity against one or more clotting factors in the treatment of hemophilia and/or for use in preventing or attenuating humoral immunity against one or more cytokines in the treatment of a viral infection and/or for use in preventing or attenuating humoral immunity against one or more cytokines in the treatment of a cancer or metastatic disease.


The present invention also provides a method of preventing or treating one or more adverse consequences of CD40L-dependent signaling in a subject, said method comprising administering an amount of a composition of the invention according to any example hereof for a time and under conditions sufficient to inhibit aberrant or inappropriate CD40L-dependent signaling. In one example, this invention provides a method of preventing or treating inflammation in a subject, said method comprising administering an amount of a composition of the invention according to any example hereof for a time and under conditions sufficient to ameliorate one or more adverse effects of CD40L-dependent signaling that contribute to an inflammatory response in a subject. In another example, the invention provides a method of preventing or treating autoimmunity in a subject, said method comprising administering an amount of a composition of the invention according to any example hereof for a time and under conditions sufficient to ameliorate one or more adverse effects of CD40L-dependent signaling that contribute to autoimmunity in a subject. In another example, this invention provides a method of preventing or treating cancer or metastatic disease in a subject, said method comprising administering an amount of a composition of the invention according to any example hereof for a time and under conditions sufficient to ameliorate one or more adverse effects of CD40L-dependent signaling that contribute to cancer in a subject.


The present invention also provides a method of treatment of any disease or condition involving a humoral immune response, said method comprising administering an amount of a composition of the invention according to any example hereof for a time and under conditions sufficient to attenuate or reduce humoral immunity against a therapeutic protein administered to the subject for treatment or prevention of the disease or condition. In such applications, the compositions may be administered concomitantly with or before or after administering the therapeutic protein to the subject. For example, this invention provides a method of treating a viral infection in a subject, said method comprising administering an amount of the composition for a time and under conditions sufficient to attenuate or reduce humoral immunity against a cytokine administered to the subject. In another example, the invention provides a method of treating hemophilia, said method comprising administering an amount of the composition for a time and under conditions sufficient to attenuate or reduce humoral immunity against a clotting factor administered to the subject.


In a related example, the present invention provides a method for inhibiting, reducing or delaying or otherwise preventing one or more CD40L-mediated events or phenotypes in a cell or a subject, said method comprising providing to the cell or subject a peptide that binds specifically to a CD40L to thereby ameliorate or inhibit or reduce or antagonize one or more CD40L-mediated events or phenotypes according to any example hereof.


In a related example, the present invention provides a method for inhibiting or otherwise modulating a CD40L-mediated signaling pathway in a cell, said method comprising contacting said cell with an effective amount of one or more CD40L peptide inhibitors of the present invention according to any embodiment hereof. In one example, the CD40L-mediated event is binding of CD40L to CD40. In another example, the CD40L-mediated event is binding of CD40L to Mac-1. In another example, the CD40L-mediated event is a cellular process mediated by CD40L binding to CD40. In another example, the CD40L-mediated event is a cellular process mediated by CD40L binding to Mac-1. In another example, the CD40L-mediated event is an inflammatory response e.g., in the vascular system, gastrointestinal system, respiratory system, nervous system, or other organ system of an animal subject e.g., as determined by a cellular response ex vivo in a cell derived from an animal subject. In accordance with this example, the method of the invention may be employed to treat or prevent an inflammatory response characterized by interaction of CD40L with CD40 and/or Mac-1 in one or more of said organs or a cell or tissue thereof, wherein an effective amount of a CD40L peptide inhibitor of the invention or an analog or derivative thereof according to any example hereof is administered to a subject in need thereof. In another example, the CD40L-mediated event is a development or complication of atherosclerosis, or formation of an atherosclerotic lesion, or aggravation or complication of an atherosclerotic lesion. In accordance with this example, the method of the invention may be employed to treat or prevent atherosclerosis or an atherosclerotic lesion or to promote a vascular repair process e.g., by inhibiting angiogenesis and/or neovascularization process(es) characterized by interaction of CD40L with CD40 and/or Mac-1, wherein an effective amount of a CD40L peptide inhibitor of the invention or an analog or derivative thereof according to any example hereof is administered to a subject in need thereof. In another example, the CD40L-mediated event is carcinogenesis or metastasis associated therewith. For example, the method of the invention may be employed to treat or prevent one or more cancers, characterized by interaction of CD40L with CD40, wherein an effective amount of a CD40L peptide inhibitor of the invention or an analog or derivative thereof according to any example hereof is administered to a subject in need thereof. Exemplary cancers the treatment of which the invention may be useful are selected from the group consisting of a non-Hodgkins lymphoma, chronic lymphocytic leukemia, multiple myeloma, B cell lymphoma, high-grade B cell lymphoma, intermediate-grade B cell lymphoma, low-grade B cell lymphoma, B cell acute lympohoblastic leukemia, myeloblastic leukemia, and Hodgkin's disease:


In a related example, the present invention provides a method for inhibiting, reducing or delaying growth or differentiation of a B-cell e.g., a normal human B-cell, said method comprising contacting said B-cell with an effective amount of one or more CD40L peptide inhibitors of the present invention according to any embodiment hereof. In one example, the present invention provides a method for inhibiting, reducing or delaying proliferation of a B-cell e.g., a normal human B cell wherein said proliferation is augmented by the interaction of a CD40 ligand with CD40 expressed on the surface of said B-cell, said method comprising contacting said B-cell with an effective amount of one or more CD40L peptide inhibitors of the present invention according to any embodiment hereof. In a further example, the present invention provides a method for inhibiting, reducing or delaying antibody production by B cells in a human patient, said method comprising administering to the subject an effective amount of one or more CD40L peptide inhibitors of the present invention according to any embodiment hereof. In a further example, the present invention provides a method for inhibiting, reducing or delaying growth of a cancer cell of B cell lineage, said method comprising contacting said B-cell with an effective amount of one or more CD40L peptide inhibitors of the present invention according to any embodiment hereof. Exemplary cancers the treatment of which the invention may be useful are selected from the group consisting of a non-Hodgkins lymphoma, chronic lymphocytic leukemia, multiple myeloma, B cell lymphoma, high-grade B cell lymphoma, intermediate-grade B cell lymphoma, low-grade B cell lymphoma, B cell acute lympohoblastic leukemia, myeloblastic leukemia, and Hodgkin's disease.


In a further related example, the present invention provides a method for inhibiting or preventing an autoimmune disease in a subject or reducing the severity of an autoimmune disease in a subject, said method comprising administering to a subject in need thereof an effective amount of one or more CD40L peptide inhibitors of the present invention according to any embodiment hereof. The autoimmune disease may be selected e.g., from the group consisting of systemic lupus erythematosus, autoimmune thrombocytopenic purpura, rheumatoid arthritis, multiple sclerosis, ankylosing spondylitis, myasthenia gravis, and pemphigus vulgaris.


In another example, the present invention provides a method of identifying or determining or predicting a secondary structure of a peptidyl inhibitor of the invention wherein said method comprises aligning primary sequence(s) of one or more peptidyl inhibitors having a predetermined activity to the primary sequence(s) of one or more known proteins or fragment(s) thereof, determining a secondary structure for the known protein(s) or fragment(s), and assigning the secondary structure for the known protein(s) or fragment(s) to the one or more peptidyl inhibitors. The process may be performed in silico. The process may comprise interrogating a secondary structure database e.g., the PDB structural database, with primary sequence data to thereby identify or resolve secondary structures and assemblies of secondary structures in silico. Alternatively, orin addition, the process may comprise interrogating a protein crystal structure database with primary sequence data to therebyidentify or resolve secondary structures and assemblies of secondary structures in silico. In another example, the process further comprises performing homology modelling and/or modelling of peptide docking on or binding to a target protein, e.g., based on the secondary structure predictions. In another example, the process further comprises performing rational drug design e.g., of small molecules based on the secondary structure predictions.





BRIEF DESCRIPTION OF THE DRAWINGS


FIG. 1 a is a schematic representation showing a CD40L peptide inhibitor derivative comprising a CD40L peptide inhibitor of the invention bound to 8-amino-3,6-dioxaoctanyl linker or spacer molecule.



FIG. 1
b is a schematic representation showing a CD40L peptide inhibitor derivative comprising the derivative of FIG. 5a wherein the linker or spacer moiety additionally comprises N-terminal cysteine.



FIG. 1
c is a schematic representation showing a CD40L peptide inhibitor derivative comprising the derivative of FIG. 5a wherein the linker or spacer moiety additionally comprises the N-terminal peptide sequence Cys-Lys-Lys (i.e., CKK).



FIG. 1
d is a schematic representation showing a CD40L peptide inhibitor derivative comprising a dimer produced between two CD40L peptide inhibitors as shown in FIG. 1b wherein the monomers are linked chemically by disulfide bridge formation between N-terminal cysteine residues.



FIG. 1
e is a schematic representation showing a CD40L peptide inhibitor derivative comprising a dimer produced between two CD40L peptide inhibitors as shown in FIG. 1c wherein the monomers are linked chemically by disulfide bridge formation between N-terminal cysteine residues.



FIG. 2 is a graphical representation showing the effect of a specific competitor comprising a soluble CD40L protein, or a non-specific competitor, on binding of the phage expressing Phylomer peptides shown on the x-axis to immobilized CD40L in a mutiwell assay format. Data indicate specific binding of all phage indicated other than the CD28 control phage, to CD40L.



FIG. 3
a is a graphical representation showing inhibition of the interaction between CD40L and CD40 by the Phylomer peptide M16 and a derivative thereof comprising an N-terminal cysteine residue (Cys-M16S) by Alphascreen proximity assay (Perkin Elmer, USA).



FIG. 3
b is a graphical representation showing inhibition of the interaction between CD40L and CD40 by the Phylomer peptide M14S by Alphascreen proximity assay (Perkin Elmer, USA).



FIG. 3
c is a graphical representation showing inhibition of the interaction between CD40L and CD40 by the Phylomer peptide M19Sa by Alphascreen proximity assay (Perkin Elmer, USA).



FIG. 3
d is a graphical representation showing inhibition of the interaction between CD40L and CD40 by the Phylomer peptide M1189Sa by Alphascreen proximity assay (Perkin Elmer, USA).



FIG. 4 is a graphical representation showing concentration-dependent inhibition of CD40L-induced CD86 expression on primary B-cells using Phylomer peptide M118 and enhanced inhibition thereof with a retroinverted form of Phylomer peptide M118 (M1-18rd) or a PEGylated form of the retroinverted peptide (mono-PEG-M118rd).



FIG. 5
a is a graphical representation showing inhibition of the interaction between CD40L and CD40 by a peptide consisting of a homodimer of the Phylomer peptide M16 as determined by Alphascreen proximity assay (Perkin Elmer, USA).



FIG. 5
b is a graphical representation showing inhibition of the interaction between CD40L and CD40 by a peptide consisting of a heterodimer of the Phylomer peptide M16 and the Phylomer peptide M14, as determined by Alphascreen proximity assay (Perkin Elmer, USA).



FIG. 5
c is a graphical representation showing inhibition of the interaction between CD40L and CD40 by several dimeric peptides relative to a control monomer peptide consisting of the M16S monomeric peptide unit or the cysteine-containing derivative thereof. Cys-M16S. Dimeric peptides included a homodimer of the Phylomer peptide M16 (6-6 dimer), a heterodimer of the Phylomer peptide M16 and the Phylomer peptide M14 (6-4 dimer), a heterodimer of the Phylomer peptide M16 and the Phylomer peptide M19 (6-9 dimer), and a heterodimer of the Phylomer peptide M16 and the Phylomer peptide M1189 (6-189 dimer). Data indicate enhanced inhibition of CD40L-CD40 interaction by binding of dimeric peptides relative to the monomeric peptide controls, as determined by Alphascreen proximity assay (Perkin Elmer, USA).



FIG. 5
d is a graphical representation showing inhibition of the interaction between CD40L and CD40 by a high purity heterodimer of the Phylomer peptide M16 and the Phylomer peptide M19 (6-9 high purity dimer), as determined by Alphascreen proximity assay (Perkin Elmer, USA).



FIG. 5
e is a graphical representation showing inhibition of the interaction between CD40L and CD40 by a high purity heterodimer of the Phylomer peptide M16 and the Phylomer peptide M1189 (6-189 high purity dimer), as determined by Alphascreen proximity assay (Perkin Elmer, USA).



FIG. 6
a is a graphical representation showing percentage inhibition of CD40L-induced CD86 expression on primary B-cells by the Phylomer peptide M118.



FIG. 6
b is a graphical representation showing lack of significant inhibition of CD40L-induced CD86 expression on primary B-cells by a control Phylomer peptide that does not bind CD40L.



FIG. 6
c is a graphical representation showing concentration-dependent inhibition of CD40L-induced CD86 expression on primary B-cells by the Phylomer peptide M118.



FIG. 6
d is a graphical representation showing concentration-dependent induction of CD40L-induced CD86 expression on primary B-cells by CD40L in a control experiment for data provided in FIGS. 6a and 6c and 6e.



FIG. 6
e is a graphical representation showing concentration-dependent inhibition of CD40L-induced CD86 expression on primary B-cells by CD40L by the Phylomer peptide M118S compared to the effect of negative controls consisting of LPS or media.



FIG. 6
f is a graphical representation showing concentration-dependent induction of CD40L-induced CD86 expression on primary B-cells by LPS in a control experiment for data provided in FIGS. 6a and 6c and 6e.



FIG. 6
g is a graphical representation showing lack of concentration-dependent inhibition of LPS-induced CD86 expression on primary B-cells by Phylomer peptide M118S, indicating that the effect of the peptides is not mediated by LPS binding to TLR 2/4.



FIG. 6
h is a graphical representation showing concentration-dependent induction of cell death by TNFα in a control experiment for data provided in FIG. 6j.



FIG. 6
i is a graphical representation showing inhibition of concentration-dependent induction of cell death by TNFα using a recombinant human TNF receptor (TNFRII) in a control experiment for data provided in FIG. 6j.



FIG. 6
j is a graphical representation showing lack of inhibition of concentration-dependent induction of cell death by TNFα using Phylomer peptides M12S, M14S, M16S, M17S, M12S, M19S and M12S, M118*S, indicating that the effect of the peptides is not mediated by TNFα.



FIG. 7
a is a graphical representation showing percentage inhibition of CD40L-induced CD86 expression on primary B-cells by a homodimer of the Phylomer peptide M16 (6-6 dimer) relative to a control monomer peptide consisting of the M1-6S monomeric peptide unit or the cysteine-containing derivative thereof. Cys-M16S. EC50 values are also indicated for each peptide. Data indicate enhanced inhibition of CD40L-induced CD86 expression by dimeric peptide relative to the monomeric peptide controls.



FIG. 7
b is a graphical representation showing percentage inhibition of CD40L-induced CD86 expression on primary B-cells by a heterodimer of the Phylomer peptide M16 and the Phylomer peptide M14 (6-4 dimer) relative to a control monomer peptide consisting of the M1-6S monomeric peptide unit or the cysteine-containing derivative thereof. Cys-M16S. EC50 values are also indicated for each peptide. Data indicate enhanced inhibition of CD40L-induced CD86 expression by dimeric peptide relative to the monomeric peptide controls.



FIG. 7
c is a graphical representation showing percentage inhibition of CD40L-induced CD86 expression on primary B-cells by several dimeric peptides relative to a control monomer peptide consisting of the M1-6S monomeric peptide unit or the cysteine-containing derivative thereof. Cys-M16S. Dimeric peptides included a homodimer of the Phylomer peptide M16 (6-6 dimer), a heterodimer of the Phylomer peptide M16 and the Phylomer peptide M14 (6-4 dimer), a heterodimer of the Phylomer peptide M16 and the Phylomer peptide M19 (6-9 dimer), and a heterodimer of the Phylomer peptide M16 and the Phylomer peptide M1189 (6-189 dimer). Data indicate enhanced inhibition of CD40L-induced CD86 expression by dimeric peptides relative to the monomeric peptide controls.



FIG. 7
d is a graphical representation showing percentage inhibition of CD40L-induced CD86 expression on primary B-cells by a heterodimer of the Phylomer peptide M16 and the Phylomer peptide M19 (6-9 dimer) relative to a control monomer peptide consisting of the M1-6S monomeric peptide unit or the cysteine-containing derivative thereof. Cys-M16S. EC50 values are also indicated for each peptide. Data indicate enhanced inhibition of CD40L-induced CD86 expression by dimeric peptide relative to the monomeric peptide controls.



FIG. 7
e is a graphical representation showing percentage inhibition of CD40L-induced CD86 expression on primary B-cells by a heterodimer of the Phylomer peptide M16 and the Phylomer peptide M1189 (6-189 dimer) relative to a control monomer peptide consisting of the M1-6S monomeric peptide unit or the cysteine-containing derivative thereof. Cys-M16S. EC50 values are also indicated for each peptide. Data indicate enhanced inhibition of CD40L-induced CD86 expression by dimeric peptide relative to the monomeric peptide controls.



FIG. 8
a is a schematic representation showing that the region of overlap between the amino acid sequences of different clones that align to the glycyl tRNA synthetases of Thermotoga maritime, Desulfovibrio vulgaris, Rhodobacter sphaeroides and Bordetella pertussis are predicted to form an anti-parallel B sheet structure. The predicted anti-parallel B sheet structure of clone CO2 40L 0504 1064 is shown.



FIG. 8
b is a schematic representation showing that the region of overlap between the amino acid sequences of different clones that align to the glycogen debranching enzyme G1gX of Rhodobacter sphaeroides are predicted to form an anti-parallel B sheet structure. The predicted anti-parallel B sheet structure of clone CO2 40L 0804 1170 is shown.



FIG. 9 is a schematic representation showing the predicted secondary structure of the peptidyl inhibitor M07 40L 0103 0859 aligned to a region of a Salmonella enterica protein.



FIG. 10 is a schematic representation showing the alignment of the predicted secondary structure for the peptidyl inhibitor M08 40L 0103 0716 with the crystal structure of a ferric alcaligin siderophore receptor. The region of the primary sequence of the ferric alcaligin siderophore receptor overlapping M08 40L 0103 0716 is as follows:









RTKQTGAYLVGRFALAEPLHLIVGDRWSDWKTKQMYFGSRREYRIKNQF





TPYAGLTYDINDTYTAYASYTEIFQPQNARDTSGGILPPIKSKSY.






Peptide M08 40L 0103 0716 comprises the following amino acid sequence:









ELRTKQTGAYLVGRFALAEPLHLMVGDRWSDWKTKQMYFGSRREYRIKN






QFTPYAGLTYDINDTYTAYASYTEIFQPQNARDTSGGILPPIKSNSc








wherein the underlined region indicates the primary sequence overlap with the ferric alcaligin siderophore receptor



FIG. 11 is a schematic representation showing the alignment of the predicted secondary structure for the peptidyl inhibitor M09 40L 0103 0755 with the crystal structure of a benzoate 1,2-dioxygenase beta subunit sequence. The region of the primary sequence of the benzoate 1,2-dioxygenase beta subunit sequence overlapping M09 40L 0103 0755 is as follows:









LYRESRLLDDKAWDAWLDCYRADAVFWMPSWDDADALVTDPQREISLI





YYPN






Peptide M09 40L 0103 0755 comprises the following amino acid sequence:









ELLcREARYLDDKDWDAWLALYAADASFWMPSWDDRDQLTEDPQREISL






IWYGN








wherein the underlined region indicates the primary sequence overlap with the benzoate


1,2-dioxygenase beta subunit sequence.





DETAILED DESCRIPTION OF THE INVENTION

CD40 and/or CD40L Signaling Inhibitors


The compositions as described herein according to any embodiment may comprise any one or more peptidyl or non-peptidyl CD40 signaling inhibitors and/or CD40L signaling inhibitors.


By “peptidyl inhibitor” is meant a composition that reduced or antagonizes the activity or effect of a stated integer e.g., CD40L or CD40 or downstream signaling from CD40L or CD40, wherein the active agent having such an inhibitory activity is a peptide.


By “non-peptidyl inhibitor” is meant a composition that reduced or antagonizes the activity or effect of a stated integer e.g., CD40L or CD40 or downstream signaling from CD40L or CD40, wherein the active agent having such an inhibitory activity is not a peptide.


For example, a peptidyl or non-peptidyl inhibitor of the present invention binds to or interacts with CD40L and inhibits CD40L-mediated activity. The peptidyl or non-peptidyl inhibitor may prevent or reduce the ability of CD40L to bind to CD40 and mediate one or more CD40-dependent signaling events. For example, a peptidyl inhibitor capable of binding to CD40L and reducing or preventing CD40-CD40L interaction will inhibit CD40-mediated Cd86 expression and/or one or more downstream CD86-mediated events. In another example, a peptidyl inhibitor capable of binding to CD40L and reducing or preventing CD40-CD40L interaction will inhibit or reduce CD40L-mediated T-cell proliferation.


As used herein, the term “CD40 signaling” shall be taken to mean one or more downstream pathway steps or effects mediated by CD40 and dependent on CD40L binding to CD40. The term “CD40L-dependent CD40-mediated” also refers to CD40 signaling as defined herein. For example, a peptidyl inhibitor of the present invention may modulate e.g., antagonize CD40 signaling by virtue of preventing CD40 from binding to CD40L.


The term “CD40L signaling” shall be taken to mean one or more downstream pathway steps or effects mediated by CD40L whether or not binding to the cognate CD40 receptor is involved. For example, a peptidyl inhibitor of the present invention may modulate e.g., antagonize CD40L signaling that does not involve CD40, by virtue of binding directly to CD40L, or alternatively, a peptidyl inhibitor of the present invention may modulate e.g., antagonize CD40L signaling that does involve CD40, by virtue of preventing CD40 from binding to CD40L.


1. Peptidyl Inhibitors of CD40 and/or CD40L Signaling


A peptidyl inhibitor described herein may be a base peptide or derivative or analog according to any example hereof, that functions as a CD40 signaling inhibitor and/or a CD40L signaling inhibitor.


The term “base, peptide” refers to a peptide in an unmodified form that possesses a stated inhibitory activity or binding activity, especially CD40L-binding activity and/or CD40L-signaling inhibitory activity and/or CD40-signaling inhibitory activity e.g., by virtue or preventing an interaction between CD40L and CD40 that activates the CD40:CD40L costimulatory pathway.


The term “derivative” or “analog” in the context of a peptidyl inhibitor refers broadly to a peptide in a modified form that possesses a stated inhibitory activity or binding activity, especially CD40L-binding activity and/or CD40L-signaling inhibitory activity and/or CD40-signaling inhibitory activity e.g., by virtue or preventing an interaction between CD40L and CD40 that activates the CD40:CD40L costimulatory pathway.


Peptide Synthesis

A peptide or an analog or derivative thereof is preferably synthesized using a chemical method known to the skilled artisan. For example, synthetic peptides are prepared using known techniques of solid phase, liquid phase, or peptide condensation, or any combination thereof, and can include natural and/or unnatural amino acids. Amino acids used for peptide synthesis may be standard Boc (Nα-amino protected Nα-t-butyloxycarbonyl) amino acid resin with the deprotecting, neutralization, coupling and wash protocols of the original solid phase procedure of Merrifield, J. Am. Chem. Soc., 85:2149-2154, 1963, or the base-labile Nα-amino protected 9-fluorenylmethoxycarbonyl (Fmoc) amino acids described by Carpino and Han, J. Org. Chem., 37:3403-3409, 1972. Both Fmoc and Boc Nα-amino protected amino acids can be obtained from various commercial sources, such as, for example, Fluka, Bachem, Advanced Chemtech, Sigma, Cambridge Research Biochemical, Bachem, or Peninsula Labs.


Generally, chemical synthesis methods comprise the sequential addition of one or more amino acids to a growing peptide chain. Normally, either the amino or carboxyl group of the first amino acid is protected by a suitable protecting group. The protected or derivatized amino acid can then be either attached to an inert solid support or utilized in solution by adding the next amino acid in the sequence having the complementary (amino or carboxyl) group suitably protected, under conditions that allow for the formation of an amide linkage. The protecting group is then removed from the newly added amino acid residue and the next amino acid (suitably protected) is then added, and so forth. After the desired amino acids have been linked in the proper sequence, any remaining, protecting groups (and any solid support, if solid phase synthesis techniques are used) are removed sequentially or concurrently; to render the final polypeptide. By simple modification of this general procedure, it is possible to add more than one amino acid at a time to a growing chain, for example, by coupling (under conditions which do not racemize chiral centers) a protected tripeptide with a properly protected dipeptide to form, after deprotection, a pentapeptide. See, e.g., J. M. Stewart and J. D. Young, Solid Phase Peptide Synthesis (Pierce Chemical Co., Rockford, Ill. 1984) and G. Barany and R. B. Merrifield, The Peptides: Analysis, Synthesis, Biology, editors E. Gross and J. Meienhofer, Vol. 2, (Academic Press, New York, 1980), pp. 3-254, for solid phase peptide synthesis techniques; and M. Bodansky, Principles of Peptide Synthesis, (Springer-Verlag, Berlin 1984) and E. Gross and J. Meienhofer, Eds., The Peptides: Analysis. Synthesis. Biology, Vol. 1, for classical solution synthesis. These methods are suitable for synthesis of a peptide of the present invention or an analog or derivative thereof.


Typical protecting groups include t-butyloxycarbonyl (Boc), 9-fluorenylmethoxycarbonyl (Fmoc) benzyloxycarbonyl (Cbz); p-toluenesulfonyl (Tx); 2,4-dinitrophenyl; benzyl (Bzl); biphenylisopropyloxycarboxy-carbonyl, t-amyloxycarbonyl, isobornyloxycarbonyl, o-bromobenzyloxycarbonyl, cyclohexyl, isopropyl, acetyl, o-nitrophenylsulfonyl and the like.


Typical solid supports are cross-linked polymeric supports. These can include divinylbenzene cross-linked-styrene-based polymers, for example, divinylbenzene-hydroxymethylstyrene copolymers, divinylbenzene-chloromethylstyrene copolymers and divinylbenzene-benzhydrylaminopolystyrene copolymers.


A peptide, analog or derivative as described herein can also be chemically prepared by other methods such as by the method of simultaneous multiple peptide synthesis. See, e.g., Houghten Proc. Natl. Acad. Sci. USA 82: 5131-5135, 1985 or U.S. Pat. No. 4,631,211.


As will be apparent to the skilled artisan based on the description herein, an analog or derivative of a peptide of the invention may comprise D-amino acids, a combination of D- and L-amino acids, and various unnatural amino acids (e.g., α-methyl amino acids, Cα-methyl amino acids, and Nα-methyl amino acids, etc) to convey special properties. Synthetic amino acids include ornithine for lysine, fluorophenylalanine for phenylalanine, and norleucine for leucine or isoleucine. Methods for the synthesis of such peptides will be apparent to the skilled artisan based on the foregoing description.


Recombinant Peptide Production

A peptide or analog or derivative thereof or fusion protein may be produced as a recombinant protein. To facilitate the production of a recombinant peptide or fusion protein nucleic acid encoding same is preferably isolated or synthesized. Typically the nucleic acid encoding the recombinant protein is/are isolated using a known method, such as, for example, amplification (e.g., using PCR or splice overlap extension) or isolated from nucleic acid from an organism using one or more restriction enzymes or isolated from a library of nucleic acids. Methods for such isolation will be apparent to the ordinary skilled artisan and/or described in Ausubel et al (In: Current Protocols in Molecular Biology. Wiley Interscience, ISBN 047 150338, 1987), Sambrook et al (In: Molecular Cloning: Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratories, New York, Third Edition 2001).


For expressing protein by recombinant means, a protein-encoding nucleic acid is placed in operable connection with a promoter or other regulatory sequence capable of regulating expression in a cell-free system or cellular system. For example, nucleic acid comprising a sequence that encodes a peptide is placed in operable connection with a suitable promoter and maintained in a suitable cell for a time and under conditions sufficient for expression to occur. Nucleic acid encoding a peptide inhibitor of CD40L-dependent signaling, including CD40L-dependent CD40-mediated signaling event(s), is described herein or is derived from the publicly available amino acid sequence.


As used herein, the term “promoter” is to be taken in its broadest context and includes the transcriptional regulatory sequences of a genomic gene, including the TATA box or initiator element, which is required for accurate transcription initiation, with or without additional regulatory elements (e.g., upstream activating sequences, transcription factor binding sites, enhancers and silencers) that alter expression of a nucleic acid (e.g., a transgene), e.g., in response to a developmental and/or external stimulus, or in a tissue specific manner. In the present context, the term “promoter” is also used to describe a recombinant, synthetic or fusion nucleic acid, or derivative which confers, activates or enhances the expression of a nucleic acid (e.g., a transgene and/or a selectable marker gene and/or a detectable marker gene) to which it is operably linked. Preferred promoters can contain additional copies of one or more specific regulatory elements to further enhance expression and/or alter the spatial expression and/or temporal expression of said nucleic acid.


As used herein, the term “in operable connection with”, “in connection with” or “operably linked to” means positioning a promoter relative to a nucleic acid (e.g., a transgene) such that expression of the nucleic acid is controlled by the promoter. For example, a promoter is generally positioned 5′ (upstream) to the nucleic acid, the expression of which it controls. To construct heterologous promoter/nucleic acid combinations (e.g., promoter/nucleic acid encoding a peptide), it is generally preferred to position the promoter at a distance from the gene transcription start site that is approximately the same as the distance between that promoter and the nucleic acid it controls in its natural setting, i.e., the gene from which the promoter is derived. As is known in the art, some variation in this distance can be accommodated without loss of promoter function.


Should it be preferred that a peptide or fusion protein of the invention is expressed in vitro a suitable promoter includes, but is not limited to a T3 or a T7 bacteriophage promoter (Hanes and Plückthun Proc. Natl. Acad. Sci. USA, 94 4937-4942 1997).


Typical expression vectors for in vitro expression or cell-free expression have been described and include, but are not limited to the TNT T7 and TNT T3 systems (Promega), the pEXP1-DEST and pEXP2-DEST vectors (Invitrogen).


Typical promoters suitable for expression in bacterial cells include, but are not limited to, the lacz promoter, the Ipp promoter, temperature-sensitive AL or AR promoters, T7 promoter, T3 promoter, SP6 promoter or semi-artificial promoters such as the IPTG-inducible tac promoter or lacUV5 promoter. A number of other gene construct systems for expressing the nucleic acid fragment of the invention in bacterial cells are well-known in the art and are described for example, in Ausubel et al (In: Current Protocols in Molecular Biology. Wiley Interscience, ISBN 047 150338, 1987), U.S. Pat. No. 5,763,239 (Diversa Corporation) and Sambrook et al (In: Molecular Cloning: Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratories, New York, Third Edition 2001).


Numerous expression vectors for expression of recombinant polypeptides in bacterial cells and efficient ribosome binding sites have been described, and include, for example, PKC30 (Shimatake and Rosenberg, Nature 292, 128, 1981); pKK173-3 (Amann and Brosius, Gene 40, 183, 1985), pET-3 (Studier and Moffat, J. Mol. Biol. 189, 113, 1986); the pCR vector suite (Invitrogen), pGEM-T Easy vectors (Promega), the pL expression vector suite (Invitrogen) the pBAD/TOPO or pBAD/thio—TOPO series of vectors containing an arabinose-inducible promoter (Invitrogen, Carlsbad, Calif.), the latter of which is designed to also produce fusion proteins with a Trx loop for conformational constraint of the expressed protein; the pFLEX series of expression vectors (Pfizer Inc., CT, USA); the pQE series of expression vectors (QIAGEN, CA, USA), or the pL series of expression vectors (Invitrogen), amongst others.


Typical promoters suitable for expression in viruses of eukaryotic cells and eukaryotic cells include the SV40 late promoter, SV40 early promoter and cytomegalovirus (CMV) promoter, CMV IE (cytomegalovirus immediate, early) promoter amongst others. Preferred vectors for expression in mammalian cells (e.g., 293, COS, CHO, 10T cells, 293T cells) include, but are not limited to, the pcDNA vector suite supplied by Invitrogen, in particular pcDNA 3.1 myc-His-tag comprising the CMV promoter and encoding a C-terminal 6×His and MYC tag; and the retrovirus vector pSRatkneo (Muller et al., Mol. Cell. Biol., 11, 1785, 1991).


A wide range of additional host/vector systems suitable for expressing a peptide or fusion protein of the present invention are available publicly, and described, for example, in Sambrook et al (In: Molecular cloning, A laboratory manual, second edition, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989).


In other examples, the peptidyl inhibitor, especially any base peptide, is expressed on by phage display, cell display, or in vitro display.


For in vitro display, the expressed peptide is linked to the nucleic acid from which it was expressed such that said peptide is presented in the absence of a host cell. For example, the peptide is displayed by ribosome display, which directly links mRNA encoded by an expression construct to the peptide that it encodes. To display a nascent polypeptide in vitro, nucleic acid encoding it is cloned downstream of an appropriate promoter (e.g., bacteriophage T3 or T7 promoter) and a ribosome binding sequence, optionally including a translatable spacer nucleic acid (e.g., encoding amino acids 211-299 of gene III of filamentous phage M13 mp 19) that stabilizes the expressed fusion protein within the ribosomal tunnel. Ribosome complexes are stabilized against dissociation from the peptide and/or its encoding mRNA by the addition of reagents such as, for example, magnesium acetate or chloroamphenicol.


For phage display, the expressed peptide is displayed on the surface of a bacteriophage, as described e.g., in U.S. Pat. No. 5,821,047 and U.S. Pat. No. 6,190,908. In general, nucleic acid comprising a sequence encoding the peptide is fused N-terminally or C-terminally to nucleic acid comprising a sequence encoding a phage coat protein e.g., M13 protein-3 (p3), M13 protein-7 (p7), or M13, protein-8 (p8).


In one example, a peptidyl inhibitor of the present invention is expressed C-terminally in a Fos fusion peptide i.e., as Fos-peptidyl inhibitor fusion in the phagemid vector pJuFo. The vector pJuFo also expresses p3 C-terminally in a c-Jun fusion peptide i.e., as c-Jun-p3. By virtue of the interaction between c-Jun and Fos, the inhibitory peptide of the present invention is displayed from pJuFo in trans as a dimer between the Fos-peptidyl inhibitor and c-Jun-p3 fusion peptides.


Alternatively, a peptidyl inhibitor of the present invention is expressed N-terminally as a p3 or p7 or p8 fusion peptide wherein the C-terminus of the peptide is fused to the N-terminus of p3 or p7 or p8. Nucleic acid encoding the peptidyl inhibitor is cloned into an insertion site in a suitable vector e.g., an EcoRI site or other restriction site, positioned such that the encoded peptidyl inhibitor is expressed as an in-frame fusion with the p3 or p7 or p8 protein.


A leader sequence e.g., PelB, comprising a translation start codon is generally positioned upstream of the insertion site. Preferably, the vector is configured so as to provide for expression of natural open reading frames in the introduced nucleic acid encoding the peptidyl inhibitor e.g., by ensuring the absence of intervening stop codons between the leader sequence and the p3 or p7 or p8 protein. The introduced nucleic acid may also be cloned in different reading frames to achieve this read-through.


Optionally, the peptidyl inhibitor-p3 or peptidyl inhibitor-p7 or peptidyl inhibitor-p8 fusion peptide is also a fusion with an intervening haemagglutinin (HA) tag moiety e.g., upstream of the p3/p7/p8 sequence and downstream of the peptidyl inhibitor in the fusion peptide. The nucleic acid encoding the HA tag moiety is generally modified to remove the amber stop codon to thereby permit translational read-through from the 5′-end of sequence encoding the peptidyl inhibitor to the p3 or p7 or 8 moiety.


Optionally, the fusion peptide comprises a cysteine residue positioned e.g., at the N-terminus of the peptidyl inhibitor moiety or at the C-terminus of the peptidyl inhibitor moiety or at the N-terminus of the p3 or p7 or p8 moiety or at the N-terminus of a HA-p3 or HA-p7 or HA-p8 moiety. In one example, the phagemid vector is engineered to provide a terminal cysteine residue or internal cysteine residue for the fusion peptide, preferably a single terminal cysteine residue or single internal cysteine residue e.g., introduced by mutation at the 5′-end of the coding sequence of p3 or p7 or p8. The presence of a single cysteine permits the expressed inhibitory peptide to form an intramolecular disulfide bridge between a sulfhydryl residue in the expressed inhibitory peptide, if present, and the N-terminal sulfhydryl residue of the p3 moiety or the p7 moiety or the p8 moiety or the HA-p3 moiety or the HA-p7 moiety or the HA-p8 moiety. Alternatively, the inclusion of a cysteine at the N-terminus or C-terminus of the peptidyl inhibitor permits the expressed inhibitory peptide to form an intramolecular disulfide bridge between the sulfhydryl residue of that cysteine and a sulfhydryl residue in the expressed inhibitory peptide, if present. It is not preferred for the phagemid vector to encode multiple cysteines in that portion of the p3 or p7 or p8 fusion peptide lacking the peptidyl inhibitor, and/or for a peptidyl inhibitor per se to comprise multiple cysteines when the phagemid vector encodes a cysteine residue in that portion of the p3 or p7 or p8 fusion peptide lacking the peptidyl inhibitor. Accordingly, in another example, the phagemid vector encodes a single cysteine in that portion of the p3 or p7 or p8 fusion peptide lacking the peptidyl inhibitor, and/or a peptidyl inhibitor comprises a single cysteine e.g., when the phagemid vector encodes a cysteine residue in that portion of the p3 or p7 or p8 fusion peptide lacking the peptidyl inhibitor.


The sequence encoding a fusion peptide according to any example hereof is displayed from an appropriate vector, e.g., a vector capable of replicating in bacterial cells. Suitable host cells e.g., E. coli, are then transformed with the recombinant vector. Said host cells are also infected with a helper phage particle encoding an unmodified form of the coat protein to which a nucleic acid fragment is operably linked. Transformed, infected host cells are cultured under conditions suitable for forming recombinant phagemid particles comprising more than one copy of the fusion protein on the surface of the particle. This system has been shown to be effective in the generation of virus particles such as, for example, a virus particle selected from the group comprising λ phage, T4 phage, M13 phage, T7 phage and baculovirus. Such phage display particles are then screened to identify a displayed protein having a conformation sufficient for binding to a target protein or nucleic acid.


Means for introducing the isolated nucleic acid molecule or a gene construct comprising same into a cell for expression are well-known to those skilled in the art. The technique used for a given organism depends on the known successful techniques. Means for introducing recombinant DNA into cells include microinjection, transfection mediated by DEAE-dextran, transfection mediated by liposomes such as by using lipofectamine (Gibco, Md., USA) and/or cellfectin (Gibco, Md., USA), PEG-mediated DNA uptake, electroporation and microparticle bombardment such as by using DNA-coated tungsten or gold particles (Agracetus Inc., WI, USA) amongst others.


In another example, the peptide derivative comprises an N-terminal and/or C-terminal cysteine residue e.g., to facilitate intramolecular cross-linking or intermolecular cross-linking with another peptide such as the same or a different peptidyl inhibitor e.g., to form a multimeric peptide, or to facilitate intermolecular cross-linking with a different moiety e.g., phage p3 protein, phage p8 protein, PEG, serum protein-binding peptide. In another example, the peptide derivative comprises an N-terminal cysteine residue. In another example, the peptide derivative comprises a C-terminal cysteine residue. In another example, the peptide derivative does not comprise both an N-terminal and a C-terminal cysteine residue e.g., because the peptide autonomously forms a stable conformation thereby avoiding the need for cyclization mediated via disulfide bond/bridge formation between N-terminal and C-terminal cysteine residues. In another example, the peptide derivative is not capable of forming or does not form intramolecular disulfide bonds/bridges or cross-links e.g., because the peptide autonomously forms a stable conformation not requiring intramolecular disulfide constraint.


Preferred CD40- and/or CD40L-signaling inhibitory peptides for use in the treatment or prophylaxis of one or more CD40L-mediated effects, especially one or more adverse consequences of aberrant CD40L-mediated signaling or CD40-mediated signaling are mimetic peptides that do not merely comprise a sequence corresponding to a fragment of a native protein that they inhibit to prevent it binding to its cognate partner or substrate e.g., they are not dominant negative mutants such as fragments of the native CD40-CD40L interface.


Protein Transduction Domains

To facilitate entry into a cell, a peptidyl inhibitor described herein, including a base peptide or derivative or analog according to any example hereof, may be conjugated to a protein transduction domain, synthesized to include a protein transduction domain, or expressed recombinantly as a fusion protein comprising a protein transduction domain. As used herein, the term “protein transduction domain” shall be taken to mean a peptide or protein that is capable of enhancing, increasing or assisting penetration or uptake of a compound conjugated to the protein transduction domain into a cell either in vitro or in vivo. Those skilled in the art will be aware that synthetic or recombinant peptides can be delivered into cells through association with a protein transduction domain such as the TAT sequence from HIV or the Penetratin sequence derived from the Antennapaedia homeodomain protein (see, for example, Temsamani and Vidal, Drug Discovery Today 9: 1012-1019, 2004, for review).


A suitable protein transduction domain will be known to the skilled artisan and includes, for example, HIV-1 TAT fragment, signal sequence based peptide 1, signal sequence based peptide 2, transportan, amphiphilic model peptide, polyarginine, or a Kaposi fibroblast growth factor (FGF) hydrophobic peptide protein transduction domain. Additional suitable protein transduction domains are described, for example, in Zhao and Weisledder Medicinal Research Reviews, 24: 1-12, 2004 and Wagstaff and Jans, Current Medicinal Chemistry, 13: 1371-1387, 2006.


A protein transduction domain is covalently attached to the N-terminus or C-terminus of a peptidyl inhibitor of the present invention, and may be a chiral analog e.g., a retroinverso peptidyl moiety or PEGylated moiety. For example, a peptidyl fusion comprising a protein transduction domain positioned N-terminal to a peptidyl inhibitor of the present invention may be produced by standard peptide synthesis means or recombinant means without the exercise of undue experimentation based on the disclosure herein. Retroinverted peptide analogs comprising a protein transduction domain positioned N-terminal to a peptidyl inhibitor of the present invention, wherein the complete sequence is retroinverted are particularly preferred and produced without inventive effort based on the disclosure herein. Peptidyl fusions comprising a protein transduction domain and a peptidyl inhibitor of the present invention may comprise a spacer or linker moiety separating protein transduction domain and peptidyl inhibitor. Alternatively, the protein transduction domain and peptidyl inhibitor may be adjacent or juxtaposed in the peptidyl fusion.


Serum Protein Moieties

As used herein, the term “serum protein moiety” shall be taken to refer to any serum protein, protein fragment or peptide having a long half life e.g., serum albumin, immunoglobulin, antibody fragment, transferrin, ferritin or other serum protein, having a long half life. By “long half life” is meant a half life in serum approximately the same as an albumin protein e.g., human serum albumin. In this respect, it is preferred for a serum protein moiety to confer on a peptidyl inhibitor of the present invention administered to a subject, including any base peptide or derivative or analog thereof, a half-life that is at least about 25% or 50% or 75% or 90% or 95% or 99% the half-life of an endogenous serum albumin protein e.g., a murine animal or primate such as a human. For example, human serum albumin has a half life in humans of 19 days e.g., Peters et al., Adv. Protein Chem. 37, 161-245 (1985), and a half-life in mice of about hours e.g., Chaudhury et al., J. Exp. Med. 197, 315-322 (2003).


A preferred serum protein moiety is an immunoglobulin fragment. By “immunoglobulin fragment” is meant any derivative of an immunoglobulin wherein the undesired effector function of Fc has been disabled or deleted, and wherein the fragment has a long half life. For example, an immunoglobulin fragment may be an Fc-disabled antibody, immunoglobulin isotype not producing undesirable side-effects, or a modified Fc not producing undesirable Fc effector function. One preferred example of an Fc-disabled antibody is a CovXBody comprising a hapten linker and Fc-disabled antibody (CovX Research LLC, San Diego Calif. 92121, USA). The peptidyl inhibitor of the present invention may be linked to a CovXBody via the hapten linker moiety of the CovXBody according to the manufacturer's instructions.


A serum protein moiety is generally covalently attached to the N-terminus or C-terminus of a peptidyl inhibitor of the present invention. For example, a peptidyl fusion comprising a serum protein moiety positioned N-terminal or C-terminal to a peptidyl inhibitor of the present invention may be produced by standard peptide synthesis means or recombinant means without the exercise of undue experimentation based on the disclosure herein. Peptidyl fusions comprising a serum protein moiety and a peptidyl inhibitor of the present invention may comprise a spacer or linker moiety separating serum protein moiety and peptidyl inhibitor. Alternatively, the serum protein moiety and peptidyl inhibitor may be adjacent or juxtaposed in the peptidyl fusion.


Particularly preferred serum protein moieties for use in the present invention are retro-inverted peptides e.g., comprising a retroinverted analog of one or more serum protein moieties.


Serum Protein-Binding Moieties

As used herein, the term “serum protein-binding moiety” shall be taken to refer to any peptide or protein having the ability to bind to a serum protein e.g., serum albumin or Fc region of an antibody or transferrin or ferritin or other serum protein having a long half life, to thereby enhance the half-life of a protein, especially a peptidyl inhibitor of the present invention. By “long half life” is meant a half life in serum approximately the same as an albumin protein e.g., human serum albumin. In this respect, it is preferred for a serum protein-binding moiety to confer on a peptidyl inhibitor of the present invention administered to a subject, including any base peptide or derivative or analog thereof, a half-life that is at least about 25% or 50% or 75% or 90% or 95% or 99% the half-life of an endogenous serum albumin protein e.g., a murine animal or primate such as a human. For example, human serum albumin has a half life in humans of 19 days e.g., Peters et al., Adv. Protein Chem. 37, 161-245 (1985), and a half-life in mice of about 35 hours e.g., Chaudhury et al., J. Exp. Med. 197, 315-322 (2003).


Peptides and proteins that comprise an amino acid sequence capable of binding to serum albumin and increase the half-life of therapeutically relevant proteins and polypeptides are known in the art. Bacterial and synthetic serum protein-binding peptides are described e.g., in International Patent Publication Nos. WO1991/0.01743, WO2001/45746 and WO2002/076489. International Patent Publication No. WO2004/041865 describes “nanobodies” directed against serum albumin that can be linked to a protein to increase its half-life. Chaudhury et al., The J. Exp. Med. 3, 315-322 (2003) describe the neonatal Fc receptor (FcRn) or “Brambell receptor” as an pH-dependent serum protein-binding moiety. US Pat. Publication 20070269422 (Ablynx N.V.) discloses nanobodies or domain antibodies (dAbs) of about 115 amino acids in length and comprising framework regions i.e., FR1 to FR4 and complementarity-determining regions i.e., CDR1 to CDR3, and which have serum half-life of at least about 50% the natural half-life of serum albumin in a primate.


Preferred serum protein-binding moieties comprise peptides that consist of or comprise an albumin-binding domain (ABD) or albumin-binding domain antibody (dAb) e.g., as described by Nguyen et al., Protein Eng, Design Sel. 19, 291-297 (2006); Holt et al., Protein Eng, Design Sel. 21, 283-288 (2008); Johnsson et al., Protein Eng, Design Sel. 21, 515-527 (2008), and US Pat. Publication No. 20070202045 (Genentech, Inc.), each of which is incorporated herein by reference.


Particularly preferred peptidyl serum protein-binding moieties for use in the present invention are retro-inverted peptides e.g., comprising a retroinverted analog of one or more serum protein-binding peptidyl moieties described in US Pat. Publication No. 20070202045 or US Pat. Publication 20070269422.


Non-peptidyl serum protein-binding moieties include e.g., clofibrate, clofibric acid, Tolmetin, Fenoprofen, Diflunisal, Etodolac, Naproxen, Nambutone, Ibuprofen, Chlorothiazide, Gemfibrozil, Nalidixic Acid, Methyldopate, Ampicillin, Cefamandole Nafate, N-(2-Nitrophenyl)-anthranilic Acid, N-Phenylanthranilic Acid and Quinidine Gluconate. The peptidyl inhibitors of the present invention may also be myristoylated, and/or modified by addition of a 4,4-diphenylcyclohexyl moiety e.g., Kurtzhals et al., Biochem. J. 312 (1995); Zobel et al., Bioorg. Med. Chem. Lett. 13, 1513 (2003).


Particularly preferred non-peptidyl serum protein-binding moieties for use in the present invention include 4-phenylbutanoic acid moieties having hydrophobic substituents on the phenyl ring and conjugated to an amino acid such as a D-amino acid e.g., 4-(p-iodophenyl)butyric acid conjugated to D-lysine through the ε-amino group e.g., Dumelin et al., Agnew. Chem. Int. Ed. 47, 3196-3201 (2008) incorporated herein by reference, and any one of a series of similar conjugates comprising 4-phenylbutanoic acid moieties. Free 4-(p-iodophenyl)butyric acid, or 4-(p-iodophenyl)butyric acid conjugated to D-lysine, is readily conjugated to a peptidyl inhibitor of the invention by condensation between hydrogen of an α-amino or ε-amino group on the peptidyl inhibitor and the hydroxyl group of the 4-(p-iodophenyl)butyric acid moiety.


A serum protein-binding moiety is generally covalently attached to the N-terminus or C-terminus of a peptidyl inhibitor of the present invention, and may be a chiral analog e.g., a retroinverso peptidyl moiety or PEGylated moiety. For example, a peptidyl fusion comprising a serum protein-binding moiety positioned N-terminal or C-terminal to a peptidyl inhibitor of the present invention may be produced by standard peptide synthesis means or recombinant means without the exercise of undue experimentation based on the disclosure herein. Retroinverted peptide analogs comprising a serum protein-binding moiety positioned N-terminal or C-terminal to a peptidyl inhibitor of the present invention, wherein the complete sequence is retroinverted are particularly preferred and produced without inventive effort based on the disclosure herein. Other peptidomimetic strategies include e.g., peptoids, N-methylated peptides etc., which are also encompassed by the present invention. Peptidyl fusions comprising a serum protein-binding moiety and a peptidyl inhibitor of the present invention may comprise a spacer or linker moiety separating serum protein-binding moiety and peptidyl inhibitor. Alternatively, the serum protein-binding moiety and peptidyl inhibitor may be adjacent or juxtaposed in the peptidyl fusion. Such configurations are readily modified by the inclusion of a protein transduction domain as described herein.


Spacers and Linkers

Each of the components of a peptidyl inhibitor described herein, including a base peptide or derivative or analog and any protein transduction domain, PEG moiety, serum protein-binding moiety according to any example hereof, may optionally be separated by a spacer or linker moiety. The spacer or linker moiety facilitates the independent folding of each of peptidyl inhibitor component, and/or provides for an appropriate steric spacing between plural peptide components and between peptidyl and non-peptidyl components. A suitable linker will be apparent to the skilled artisan. For example, it is often unfavorable to have a linker sequence with high propensity to adopt α-helix or β-strand structures, which could limit the flexibility of the protein and consequently its functional activity. Rather, a more desirable linker is a sequence with a preference to adopt extended conformation. In practice, most currently designed linker sequences have a high content of glycine residues that force the linker to adopt loop conformation. Glycine is generally used in designed linkers because the absence of a β-carbon permits the polypeptide backbone to access dihedral angles that are energetically forbidden for other amino acids.


Preferably, the linker is hydrophilic, i.e. the residues in the linker are hydrophilic.


In another example, a linker is a glycine residue or polyglycine moiety or polyserin moiety. Linkers comprising glycine and/or serine have a high freedom degree for linking of two proteins, i.e., they enable the fused proteins to fold and produce functional proteins. Robinson and Sauer Proc. Natl. Acad. Sci. 95: 5929-5934, 1998 found that it is the composition of a linker peptide that is important for stability and folding of a fusion protein rather than a specific sequence.


In one example, linkers join, identical peptide target binding moieties to form homodimers. In another example, linkers join different peptide target binding moieties to form heterodimers. In another example, the linker separates a peptidyl inhibitor of the invention from a protein transduction domain. In another example, the linker separates, a peptidyl inhibitor of the invention from a PEG moiety. In another example, the linker separates a peptidyl inhibitor of the invention from a HES moiety. In another example, the linker separates a peptidyl inhibitor of the invention from a polyglycine moiety. In another example, the linker separates a peptidyl inhibitor of the invention from a serum protein moiety. In another example, the linker separates a peptidyl inhibitor of the invention from a serum protein-binding moiety. In another example, the linker separates a protein transduction domain from a PEG moiety, HED moiety, polyglycine moiety, serum protein moiety or serum protein-binding moiety.


Peptidyl linkers may also be derivatized or analogs prepared there from according to standard procedures described herein.


Base Peptides

In one example, a base peptide comprises an amino acid sequence set forth in any one of SEQ ID NOs: 1 to 18 or 44, or SEQ ID Nos: 45 to 330, or SEQ ID Nos: 331 to 393.


Peptide Derivatives

The present invention also encompasses a derivative of a peptide inhibitor of CD40 and/or CD40L signaling.


As used herein the term “derivative” shall be taken to mean a peptide that is derived from an inhibitory peptide of the invention as described herein e.g., a fragment or processed form of the peptide, wherein the active portion of the base peptide is not modified e.g., a functional fragment.


As used herein the term “functional fragment” shall be taken to mean a fragment of a peptide or analog thereof that is capable of binding to CD40L and/or reducing or preventing CD40L binding to CD40 and/or reducing or preventing CD40-mediated signaling and/or CD40L-mediated signaling. In this respect, the activity of a functional fragment need not equivalent to the based peptide (or an analog) from which it is derived. For example, the fragment may have slightly enhanced or reduced activity compared to the peptide or analog from which it is derived by virtue of the removal of flanking sequence.


The term “derivative” also encompasses fusion proteins comprising a peptide of the invention. For example, the fusion protein comprises a label, such as, for example, an epitope, e.g., a FLAG epitope or a V5 epitope or an HA epitope. For example, the epitope is a FLAG epitope. Such a tag is useful for, for example, purifying the fusion protein. Alternatively, or in addition, a derivative in this context may comprise a peptidyl protein transduction domain and/or serum protein-binding peptide or domain. The term “derivative” also encompasses a derivatized peptide, such as, for example, a peptide modified to contain one or more-chemical moieties other than an amino acid. The chemical moiety may be linked covalently to the peptide e.g., via an amino terminal amino acid residue, a carboxyl terminal amino acid residue, or at an internal amino acid residue. Such modifications include the addition of a protective or capping group on a reactive moiety in the peptide, addition of a detectable label, and other changes that do not adversely destroy the activity of the peptide compound. For example, a derivative may comprise a PEG moiety, radionuclide, colored latex, etc.


A derivative generally possesses or exhibits an improved characteristic relative to a e.g., enhanced protease resistance and/or longer half-life and/or enhanced transportability between cells or tissues of the human or animal body and/or reduced adverse effect(s) and/or enhanced affinity for CD40L and/or enhanced CD40L-signaling inhibitory activity and/or enhanced CD40-signaling inhibitory activity.


The following examples of peptide derivatives may be employed separately or in combination using standard procedures known to the skilled artisan.


In one example, a peptide derivative comprises a polyethylene glycol (PEG) moiety e.g., having a molecular mass of about 5 kDa or about 12 kDa or about 20 kDa or about kDa or about 40 kDa. The PEG moiety may comprise a branched or unbranched molecule. A PEG moiety may be added to the N-terminus and/or to the C-terminus of a peptidyl inhibitor of the invention, including a peptide, derivative or analog thereof as described according to any example herein. A PEG moiety may enhance serum half-life of the peptidyl inhibitor e.g., by protecting the peptide from degradation. A PEG moiety may be separated from the N-terminus and/or C-terminus of the peptidyl inhibitor by a spacer e.g., comprising up to 6 or 7 or 8 or 9 or 10 carbon atoms such as an 8-amino-3,6-dioxaoctanoyl spacer. For example, a spacer may reduce steric hindrance of the inhibition of CD40 or CD40L signaling or reduce steric hindrance of inhibition of the CD40-CD40L interaction. Maleimide chemistry may be employed to conjugate a PEG moiety to the peptide e.g., via cysteine residues located either within or at the N-terminal end of the peptide. For peptides that are refractory to conjugation in this manner e.g., by virtue of intramolecular disulfide bridge formation, a variety of other chemistries known to the skilled artisan may be employed to ligate PEG moieties onto the N-terminal and/or C-terminal ends of the peptides.


In another example, a peptide derivative comprises a hydroxyethyl starch (HES) moiety i.e., the peptidyl inhibitor is “HESylated”. The HES moiety may comprise a branched or unbranched molecule. A HES moiety may be added to the N-terminus and/or to the C-terminus of a peptidyl inhibitor of the invention, including a peptide, derivative or analog thereof as described according to any example herein. A HES moiety may enhance serum half-life of the peptidyl inhibitor e.g., by protecting the peptide from degradation. A HES moiety may be separated from the N-terminus and/or C-terminus of the peptidyl inhibitor by a spacer e.g., comprising up to 6 or 7 or 8 or 9 or 10 carbon atoms such as an 8-amino-3,6-dioxaoctanoyl spacer. For example, a spacer may reduce steric hindrance of the inhibition of CD40 or CD40L signaling or reduce steric hindrance of inhibition of the CD40-CD40L interaction. Maleimide chemistry may be employed to conjugate a HES moiety to the peptide e.g., via cysteine residues located either within or at the N-terminal end of the peptide. For peptides that are refractory to conjugation in this manner e.g., by virtue of intramolecular disulfide bridge formation, a variety of other chemistries known to the skilled artisan may be employed to ligate HES moieties onto the N-terminal and/or C-terminal ends of the peptides.


In another example, a peptide derivative comprises a polyglycine moiety e.g., comprising two or three or four or five or six or seven or eight or nine or ten glycine residues covalently linked. A polyglycine moiety may be added to the N-terminus and/or to the C-terminus of a peptidyl inhibitor of the invention, including a peptide, derivative or analog thereof as described according to any example herein, to produce a “polyglycinated” peptide. A polyglycine moiety may enhance serum half-life of the peptidyl inhibitor e.g., by protecting the peptide from degradation. A polyglycine moiety may be further separated from the N-terminus and/or C-terminus of the peptidyl inhibitor by a spacer e.g., comprising up to 6 or 7 or 8 or 9 or 10 carbon atoms such as an 8-amino-3,6-dioxaoctanoyl spacer. Standard recombinant means, oxime chemistry or peptide synthetic means are employed to add a polyglycine moiety to a peptidyl inhibitor of the present invention. A polyglycine moiety may also be used in conjunction with another moiety to extend the half-life of a peptidyl inhibitor of the present invention as described according to any example hereof, wherein the polyglycine moiety itself may serve further as a spacer between the peptidyl inhibitor and the other moiety.


In another example, a peptide derivative comprises a serum protein moiety or serum protein-binding moiety as described according to any example hereof, which may be added to the N-terminus and/or to the C-terminus of a peptidyl inhibitor of the invention, including a peptide, derivative or analog thereof as described according to any example herein. A serum protein moiety or serum protein-binding moiety may enhance serum half-life of the peptidyl inhibitor or translocation of the peptide in serum. A serum protein moiety or serum protein-binding moiety may be separated from the N-terminus and/or C-terminus of the peptidyl inhibitor by a spacer e.g., comprising up to 6 or 7 or 8 or 9 or 10 carbon atoms such as an 8-amino-3,6-dioxaoctanoyl spacer. For example, a spacer may reduce steric hindrance of the inhibition of CD40 or CD40L signaling or reduce steric hindrance of inhibition of the CD40-CD40L interaction.


In another example, a peptide derivative comprises a moiety e.g., a peptide, capable of inhibiting binding to Fc and/or otherwise reducing adverse consequences of activating CD40. Such a moiety may be added to the N-terminus and/or to the C-terminus of a peptidyl inhibitor of the invention, including a peptide, derivative or analog thereof as described according to any example herein. Such a moiety may be separated from the N-terminus and/or C-terminus of the peptidyl inhibitor by a spacer e.g., comprising up to 6 or 7 or 8 or 9 or 10 carbon atoms such as an 8-amino-3,6-dioxaoctanoyl spacer. For example, a spacer may reduce steric hindrance of the inhibition of CD40 or CD40L signaling or reduce steric hindrance of inhibition of the CD40-CD40L interaction.


In another example, the peptide derivative comprises an N-terminal and/or C-terminal cysteine residue e.g., to facilitate intramolecular cross-linking or intermolecular cross-linking with another peptide such as the same or a different peptidyl inhibitor e.g., to form a multimeric peptide, or to facilitate intermolecular cross-linking with a different moiety e.g., phage p3 protein, phage p8 protein, serum protein moiety or serum protein-binding moiety. In another example, the peptide derivative comprises an N-terminal cysteine residue. In another example, the peptide derivative comprises a C-terminal cysteine residue. In another example, the peptide derivative does not comprise both an N-terminal and a C-terminal cysteine residue e.g., because the peptide autonomously forms a stable conformation thereby avoiding the need for cyclization mediated via disulfide bond/bridge formation between N-terminal and C-terminal cysteine residues.


In another example, the peptide derivative is not capable of forming or does not form intramolecular disulfide bonds/bridges or cross-links e.g., because the peptide autonomously forms a stable conformation not requiring intramolecular disulfide constraint.


In another example, the peptide derivative comprises a plurality of peptides of the present invention. Such “chain-extended” variants may bind to CD40L with higher affinity than the monomeric base peptide e.g., by virtue of the larger ligand binding to CD40L over a larger surface area but within the same proximity as the monomeric base peptide. Such chain extended variants may also exhibit increased affinity for CD40L over their monomeric constituent sequences by virtue of increasing local inhibitor concentration and/or by concurrent binding to adjacent CD40L subunits or adjacent sites on the same subunit, thereby benefiting from an avidity effect.


Methods for producing multimeric proteins include conventional peptide synthesis and recombinant expression means.


In one preferred example, the ligand-binding interface with CD40L is extended by directed evolution to achieve multimerization. In this example, a second peptidyl inhibitor is positioned downstream of a first peptidyl inhibitor, wherein said positioning is adjacent or spaced apart e.g., by one or more amino acids or other linker/spacer molecule as described herein.


Alternatively, multimeric proteins are produced by expression in a single phagemid vector having one or more recombination sites for a site-specific recombinase e.g., one or more attachment sites selected from the group consisting of lox, RS, gix, frt, attB, attP, attL and attR. The phagemid vector may be a modified form of any phagemid vector suitable for phage display methodology, the only requirement being the addition of one or more attachment sites for a site-specific recombinase. For example, the filamentous phage may be a single stranded DNA bacteriophage vector, a modified phagemid pIII (syn. P3) or PVIII (syn. P8) display vector comprising one or more attachment sites for a site-specific recombinase, a modified phagemid gill display vector comprising one or more attachment sites for a site-specific recombinase, a modified pJUFO vector comprising one or more attachment sites for a site-specific recombinase, or a modified pLUCK vector comprising one or more attachment sites for a site-specific recombinase. A preferred form of a suitable phagemid vector for expressing multimeric peptide comprises:


(i) one or more recombination sites for a site-specific recombinase positioned so as to provide for recombination between said vector and a nucleic acid molecule encoding one or more amino acid sequences to be expressed from said vector wherein said recombination provides for expression of a fusion protein between said one or more amino acid sequences and a filamentous phage protein e.g., p3 or p8;


(ii) a promoter e.g., a lambda PL promoter, positioned so as to be capable of regulating transcription of nucleic acid encoding the fusion protein at (i);


(iii) at least one sequence capable of terminating transcription of nucleic acid encoding the fusion protein at (i);


(iv) a replication origin derived from a filamentous phage;


(v) a plasmid replication origin; and


(vi) at least one selection marker.


In one example, the phagemid vector comprises two recombination sites for a site-specific recombinase wherein each of said recombination sites is positioned so as to provide for recombination between said vector and a nucleic acid molecule encoding one or more amino acid sequences to be expressed from said vector wherein said recombination provides for expression of a fusion protein between said one or more amino acid sequences and a filamentous phage protein e.g., p3 or p8.


Isolated nucleic acid encoding one or more amino acid sequences for multimerization are sub-clones into the phagemid vector, wherein said nucleic acid comprises one or more recombination sites for a site-specific recombinase compatible with one or more recombination sites for the same site-specific recombinase on the phagemid vector. For example, the isolated nucleic acid may encode one or more amino acid sequences wherein each of said amino acid sequences is capable of forming secondary structures and/or super-secondary structures, e.g., a peptidyl inhibitor of the invention, or secondary structure as shown in Table 1 hereof, a fold, or an assembly of secondary structures. Exemplary nucleic acids encode peptide inhibitors of one or more protein-protein interaction(s) and/or one or more biological phenotypes attributable to said protein-protein interaction(s) e.g., nucleic acid encoding one or more CD40L peptide inhibitors of the present invention as described according to any example hereof. Wherein the isolated nucleic acid encodes a single amino acid sequence, the recombination site(s) will generally be positioned at one end- or so as to flank the sequence encoding the amino acid sequence being introduced to the vector. Wherein the isolated nucleic acid encodes a plurality of amino acid sequences to be expressed by phage display, the recombination sites will generally be positioned at one end or so as to flank each nucleotide sequence encoding an amino acid sequence being introduced to the vector, or alternatively, flanking the nucleic acid encoding all of the amino acid sequences being introduced to the vector.


The isolated nucleic acid introduced to the vector may comprise a spacer nucleotide sequence, e.g., encoding an amino acid spacer such as a GS linker, to provide for spatial separation between amino acid sequences of interest in the expressed multimeric protein and/or to provide for spatial separation between the nucleotide sequence encoding amino acid sequences of interest and recombination sites e.g., to prevent spurious recombination of functionally-important coding sequence.


In a further example, a plurality of peptides is expressed in a single phagemid vector, by a method comprising:


(i) providing a phagemid vector having one or more recombination sites for a site-specific recombinase said sites being positioned so as to provide for recombination between said vector and a nucleic acid molecule encoding one or more amino acid sequences to be expressed from said vector;


(ii) providing isolated nucleic acid encoding one or more amino acid sequences and wherein said nucleic acid comprises one or more recombination sites for a site-specific recombinase compatible with one or more recombination sites for the same site-specific recombinase on the phagemid vector to which said nucleic acid is to be introduced;


(iii) causing recombination to occur between said one or more recombination sites for a site-specific recombinase at (i) and said one or more recombination sites for a site-specific recombinase at (ii) to thereby produce a phagemid vector capable of expressing of a fusion protein between said one or more amino acid sequences at (ii) and a filamentous phage protein e.g., p3 or p8;


(iv) providing the phagemid vector produced at (iii) and isolated nucleic acid encoding one or more amino acid sequences, wherein said isolated nucleic acid comprises one or more recombination sites for a site-specific recombinase compatible with one or more recombination sites for the same site-specific recombinase on the phagemid vector produced at (iii); and


(v) causing recombination to occur between recombination sites for a site-specific recombinase at (iv) to thereby produce a phagemid vector capable of expressing of a fusion protein between a plurality of amino acid sequences and a filamentous phage protein e.g., p3 or p8.


An integrase e.g., a tyrosine integrase, or serine recombinase may be employed to facilitate recombination events, the only requirements being compatibility of the site-specific recombination sites between the phagemid vector and nucleic acid to be introduced, and the enzyme(s) or enzyme complex(es) used to promote excision and ligation events. Such compatible recombinase systems are known to those skilled in the art when provided with suitable site-specific recombination systems, or described herein, including Sin resolvase, ΦRv1 integrase, lambda integrase, Cre recombinase, R recombinase, Gin recombinase and FLP recombinase, amongst others.


Two or three or four or five or six or more peptides may be recombined into the same fusion protein for expression with a filamentous phage protein. A limitation to the number of monomers that are introduced into the phage vector may be provided by stability considerations from highly repetitive sequences in the case of homomeric proteins, and the number of units provided in the isolated nucleic acid being introduced.


Another example of the present invention provides an isolated phagemid vector having one or more recombination sites for a site-specific recombinase said sites being positioned so as to provide for recombination between said vector and a nucleic acid molecule encoding one or more amino acid sequences to be expressed from said vector. In one example, the isolated phagemid vector comprises one or more recombination sites for a site-specific recombinase positioned so as to provide for recombination between said vector and a nucleic acid molecule encoding one or more amino acid sequences to be expressed from said vector wherein said recombination provides for expression of a fusion protein between said one or more amino acid sequences and a filamentous phage protein e.g., p3 or p8. In another example, the isolated phagemid vector comprises:


(i) one or more recombination sites for a site-specific recombinase positioned so as to provide for recombination between said vector and a nucleic acid molecule encoding one or more amino acid sequences to be expressed from said vector wherein said recombination provides for expression of a fusion protein between said one or more amino acid sequences and a filamentous phage protein e.g., p3 or p8;


(ii) a promoter e.g., a lambda PL promoter, positioned so as to be capable of regulating transcription of nucleic acid encoding the fusion protein at (i);


(iii) at least one sequence capable of terminating transcription of nucleic acid encoding the fusion protein at (i);


(iv) a replication origin derived from a filamentous phage;


(v) a plasmid replication origin; and


(vi) at least one selection marker.


The phagemid vector may comprise one or more recombination sites for a site-specific recombinase wherein a recombination site is selected from the group consisting of lox, RS, gix, frt, attB, atiP, attL and attR.


In one example, the phagemid vector is a single stranded DNA bacteriophage vector. In another example, the phagemid vector is a modified phagemid p3 or p8 display vector comprising one or more attachment sites for a site-specific recombinase. In a further example, the phagemid vector is a modified phagemid gIII display vector comprising one or more attachment sites for a site-specific recombinase. In a further example, the phagemid vector is a modified pJUFO vector comprising one or more attachment sites for a site-specific recombinase. In another example, the phagemid vector is a modified pLUCK vector comprising one or more attachment sites for a site-specific recombinase.


In a further example, the present invention provides for the use of a phagemid vector of the present invention in the expression of a synthetic multimeric protein by phage display. In one example, a combinatorial protein will comprise a plurality of peptide monomers each monomer having the same binding affinity/and/or substrate specificity and/or or functionality. The peptide monomers may be closely-related by sequence or divergent e.g., variants of the same base peptide sequence or derived from different peptides. In another example, a combinatorial protein will comprise a plurality of peptide monomers each monomer having a different binding affinity and/or substrate specificity and/or or functionality e.g., wherein the peptide monomers are divergent at the sequence level and/or derived from different peptides.


It will be apparent to the skilled artisan that means for derivation of a peptide apply equally to any peptidyl inhibitor of the invention, an analog thereof, and any additional peptidyl components of a fusion peptide e.g., a protein transduction domain and/or peptidyl linker or spacer and/or serum protein moiety and/or serum protein-binding moiety to which the peptidyl inhibitor(s) and/or analog(s) is/are attached.


Peptide Analogs

In another example of the invention, a CD40 and/or CD40L signaling inhibitor is a peptide analog.


As used herein, the term “analog” shall be taken to mean a peptide wherein the active portion is modified e.g., to comprise one or more naturally-occurring and/or non-naturally-occurring amino acids, provided that the peptide analog is capable of inhibiting or reducing CD40 and/or CD40L signaling. For example, the term “analog” encompasses an inhibitory peptide comprising one or more conservative amino acid changes. In another example, an “analog” comprises one or more D-amino acids.


An analog generally possesses or exhibits an improved characteristic relative to a base peptide from which it is derived e.g., enhanced protease resistance and/or longer half-life and/or enhanced transportability between cells or tissues of the human or animal body and/or reduced adverse effect(s) and/or enhanced affinity for CD40L and/or enhanced CD40L-signaling inhibitory activity and/or enhanced CD40-signaling inhibitory activity.


Suitable peptide analogs include, for example, a peptide comprising one or more conservative amino acid substitutions. A “conservative amino acid substitution” is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art, including basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), β-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine).


It also is contemplated that other sterically similar compounds may be formulated to mimic the key portions of the peptide structure. Such compounds, which may be termed peptidomimetics, may be used in the same manner as a CD40 and/or CD40L signaling peptide inhibitor or derivative thereof. The generation of such an analog may be achieved by the techniques of modeling and chemical design known to those of skill in the art. It will be understood that all such sterically similar peptide analogs fall within the scope of the present invention.


An example of an analog of a peptide of the invention comprises one or more non-naturally occurring amino acids or amino acid analogs. For example, a peptide inhibitor as described herein comprises one or more naturally occurring non-genetically encoded L-amino acids, synthetic L-amino acids or D-enantiomers of an amino acid. For example, the peptide comprises only D-amino acids. For example, the analog comprises one or more residues selected from the group consisting of: hydroxyproline, β-alanine, 2,3-diaminopropionic acid, α-aminoisobutyric acid, N-methylglycine (sarcosine), ornithine, citrulline, t-butylalanine, t-butylglycine, N-methylisoleucine, phenylglycine, cyclohexylalanine, norleucine, naphthylalanine, pyridylananine 3-benzothienyl alanine 4-chlorophenylalanine, 2-fluorophenyl alanine, 3-fluorophenylalanine, 4-fluorophenylalanine, penicillamine, 1,2,3,4-tetrahydro-tic isoquinoline-3-carboxylic acid β-2-thienylalanine, methionine sulfoxide, homoarginine, N-acetyl lysine, 2,4-diamino butyric acid, p-aminophenylalanine, N-methylvaline, homocysteine, homoserine, ε-amino hexanoic acid, δ-amino valeric acid, 2,3-diaminobutyric acid and mixtures thereof.


Other amino acid residues that are useful for making the peptides and peptide analogs described herein can be found, e.g., in Fasman, 1989, CRC Practical Handbook of Biochemistry and Molecular Biology, CRC Press, Inc., and the references cited therein.


The present invention additionally encompasses an isostere of a peptide described herein. The term “isostere” as used herein is intended to include a chemical structure that can be substituted for a second chemical structure because the steric conformation of the first structure fits a binding site specific for the second structure. The term specifically includes peptide back-bone modifications (i.e., amide bond mimetics) known to those skilled in the art. Such modifications include modifications of the amide nitrogen, the α-carbon, amide carbonyl, complete replacement of the amide bond, extensions, deletions or backbone crosslinks. Several peptide backbone modifications are known, including ψ[CH2S], ψ[CH2NH], ψ[CSNH2], ψ[NHCO], ψ[COCH2], and ψ[(E) or (Z) CH═CH]. In the nomenclature used above, ψ indicates the absence of an amide bond. The structure that replaces the amide group is specified within the brackets.


Other modifications include, for example, an N-alkyl (or aryl) substitution (ψ [CONR]), or backbone crosslinking to construct lactams and other cyclic structures. Other derivatives of the modulator compounds of the invention include C-terminal hydroxymethyl derivatives, O-modified derivatives (e.g., C-terminal hydroxymethyl benzyl ether), N-terminally modified derivatives including substituted amides such as alkylamides and hydrazides.


In another example, a peptide analog is a retro-peptide analog (see, for example, Goodman et al., Accounts of Chemical Research, 12:1-7, 1979). A retro-peptide analog comprises a reversed amino acid sequence of a peptide inhibitor described herein. For example, a retro-peptide analog of a peptide inhibitor comprises a reversed amino acid sequence of a sequence set forth in any one of SEQ ID NOs: 35 to 43 or a reversed sequence of any one of SEQ ID NOs: 1 to 34 or 44. Optionally, the peptide analog comprises an additional feature, such as, for example, a protein transduction domain and/or serum protein moiety and/or serum protein-binding moiety, each of which may also be a retro-peptide analog. The retro-peptide analog according to any example hereof may be PEGylated.


In a further example, an analog of a peptide described herein is a retro-inverso peptide (as described, for example, in Sela and Zisman, FASEB J. 11:449, 1997). Evolution has ensured the almost exclusive occurrence of L-amino acids in naturally occurring proteins. As a consequence, virtually all proteases cleave peptide bonds between adjacent L-amino acids. Accordingly, artificial proteins or peptides composed of D-amino acids are preferably resistant to proteolytic breakdown. Retro-inverso peptide analogs are isomers of linear peptides in which the direction of the amino acid sequence is reversed (retro) and the chirality, D- or L-, of one or more amino acids therein is inverted (inverso) e.g., using D-amino acids rather than L-amino acids, e.g., Jameson et al., Nature, 368, 744-746 (1994); Brady et al., Nature, 368, 692-693 (1994). The net result of combining D-enantiomers and reverse synthesis is that the positions of carbonyl and amino groups in each amide bond are exchanged, while the position of the side-chain groups at each alpha carbon is preserved. An advantage of retro-inverso peptides is their enhanced activity in vivo due to improved resistance to proteolytic degradation, i.e., the peptide has enhanced stability. (e.g., Chorev et al., Trends Biotech. 13, 438-445, 1995).


Retro-inverso peptide analogs may be complete or partial. Complete retro-inverso peptides are those in which a complete sequence of a peptide descried herein is reversed and the chirality of each amino acid in a sequence is inverted, other than glycine, because glycine does not have a chiral analog. Partial retro-inverso peptide analogs are those in which only some of the peptide bonds are reversed and the chirality of only those amino acid residues in the reversed portion is inverted. For example, one or two or three or four or five or six or seven or eight or nine or ten or eleven or twelve or thirteen or fourteen or fifteen or sixteen or seventeen or eighteen or nineteen or twenty or twenty one or twenty two or twenty three or twenty four or twenty five or twenty six or twenty seven or twenty eight or twenty nine or thirty or thirty one or thirty two or thirty three or thirty four or thirty five or thirty six or thirty seven or thirty eight amino acid residues are D-amino acids. The present invention clearly encompasses both partial and complete retro-inverso peptide analogs.


In this respect, such a retroinverso peptide analog may optionally include an additional component, such as, for example, a protein transduction domain, which may also be retroinverted.


For example, a retro-inverso peptide analog comprises an amino acid sequence set forth in any one of SEQ ID NOs: 35 to 43, or a retro-inverso peptide analog of any one of SEQ ID NOs: 1 to 34 or 44, or SEQ ID Nos: 45 to 330, or SEQ ID Nos: 331 to 393. Optionally, a retro-inverso peptide analog comprises an additional feature, such as, for example, a protein transduction domain and/or serum protein moiety and/or serum protein-binding moiety, each of which may also be a retro-peptide analog. The retro-inverso peptide analog according to any example hereof may also be PEGylated, HESylated or polyglycinated.


In yet another example, a base peptide is mutated to thereby improve the bioactivity of the peptide, e.g., the affinity with which the peptide binds to a target molecule and/or the specificity with which a peptide binds to a target molecule. Methods for mutating a peptide will be apparent to the skilled artisan and/or are described herein an include e.g., affinity maturation. For example, diverse amino acid sequences may be derived from a base peptide and peptides produced, by synthetic or recombinant means.


For affinity maturation employing synthetic means, the amino acid sequences of a peptide inhibitor is modified in silico e.g., so as to retain secondary structure characteristics of the base peptide, a data set of related sequences is produced, and the peptides are synthesized and screened for activity.


For affinity maturation employing recombinant means, it is necessary to mutate nucleic acids encoding a diverse set of amino acid sequences by site-directed or random mutagenesis approaches. For example, nucleic acid may be amplified using mutagenic PCR such as by (i) performing the PCR reaction in the presence of manganese; and/or (ii) performing the PCR in the presence of a concentration of dNTPs sufficient to result in misincorporation of nucleotides. Methods of inducing random mutations using PCR are known in the art and are described, for example, in Dieffenbach (ed) and Dveksler (ed) (In: PCR Primer: A Laboratory Manual, Cold Spring Harbour Laboratories, NY, 1995). Furthermore, commercially available kits for use in mutagenic PCR are obtainable, such as, for example, the Diversify PCR Random Mutagenesis Kit (Clontech) or the GeneMorph Random Mutagenesis Kit (Stratagene). For example, a PCR reaction is performed in the presence of at least about 200 μM manganese or a salt thereof, more preferably at least about 300 μM manganese or a salt thereof, or even more preferably at least about 500 μM or at least about 600 μM manganese or a salt thereof. Such concentrations manganese ion or a manganese salt induce from about 2 mutations per 1000 base pairs (bp) to about 10 mutations every 1000 bp of amplified nucleic acid (Leung et al Technique 1, 11-15, 1989).


Alternatively, nucleic acid is mutated by inserting said nucleic acid into a host cell that is capable of mutating nucleic acid. Such host cells are deficient in one or more enzymes, such as, for example, one or more recombination or DNA repair enzymes, thereby enhancing the rate of mutation to a rate that is rate approximately 5,000 to 10,000 times higher than for non-mutant cells. Strains particularly useful for the mutation of nucleic acids carry alleles that modify or inactivate components of the mismatch repair pathway. Examples of such alleles include alleles selected from the group consisting of mutY, mutM, mutD, mutT, mutA, mutC and mutS. Bacterial cells that carry alleles that modify or inactivate components of the mismatch repair pathway are known in the art, such as, for example the XL-1Red, XL-mutS and XL-mutS-Kanr bacterial cells (Stratagene).


It will also be apparent to the skilled artisan that unitary analogs may be produced from any peptidyl inhibitor of the invention, with or without any other peptidyl moieties covalently attached to the inhibitor e.g., as an analog of a fusion peptide comprising e.g., one or more peptidyl inhibitors and an element selected from a protein transduction domain and/or peptidyl linker or spacer and/or serum protein moiety and/or serum protein-binding peptide moiety to which the peptidyl inhibitor(s) is/are attached. Such unitary analogs may be derivatized as described herein.


2. Non-peptidyl inhibitors of CD40 and/or CD40L signaling


A non-peptidyl inhibitor described herein may be a nucleic acid or small molecule or a derivative or analog thereof according to any example hereof, that functions as a CD40 signaling inhibitor and/or a CD40L signaling inhibitor. Preferred non-peptidyl inhibitors of the present invention are functional equivalents of a peptidyl inhibitor of the present invention, however they preferably possess enhanced inhibitory activity or affinity for CD40L, or enhanced pharmaceutical properties e.g., longer half-life, enhanced uptake and/or transportability between cells or tissues of the animal body and/or suitability for a particular mode of administration e.g., injectability, inhalability or modified solubility characteristic. Antibody inhibitors are less preferred.


As with peptidyl inhibitors, a non-peptidyl inhibitor of the present invention will reduce or prevent CD40L-binding activity and/or CD40L-signaling inhibitory activity and/or CD40-signaling inhibitory activity e.g., by virtue or preventing an interaction between CD40L and CD40 that activates the CD40:CD40L costimulatory pathway.


The term “derivative” or “analog” in the context of a non-peptidyl inhibitor refers broadly to a non-peptidyl composition in a modified form compared to the inhibitory molecule from which it is derived and retains inhibitory activity or possesses enhanced inhibitory activity with respect to CD40L-binding and/or CD40L-signaling and/or CD40-signaling e.g., by virtue or preventing an interaction between CD40L and CD40 that activates the CD40:CD40L costimulatory pathway. A derivative or analog need not possess equivalent inhibitory activity compared to the molecule (or peptide) from which it is derived.


In one example of the invention, a non-peptidyl inhibitor of CD40 and/or CD40L signaling comprises nucleic acid that reduces or prevents the interaction between CD40 and CD40L e.g., by binding to CD40L at or near the interaction interface or other site required for CD40 and/or CD40L signaling.


In one example, a CD40L-signaling inhibitor or CD40-signaling inhibitor is a small molecule. The present invention thus includes a small molecule inhibitor and/or uses thereof for the treatment and/or prophylaxis of one or more conditions associated with aberrant CD40L-signaling or aberrant CD40-signaling and complications thereof. For example, a small molecule inhibitor is used in the preparation of a medicament for the treatment or prophylaxis of one or more conditions associated with aberrant CD40L-signaling or aberrant CD40-signaling and/or complications thereof.


A suitable small molecule inhibitor is identified from a library of small molecules. Techniques for synthesizing small organic compounds will vary considerably depending upon the compound, however such methods will be well known to those skilled in the art. In one embodiment, informatics is used to select suitable chemical building blocks from known compounds, for producing a combinatorial library. For example, QSAR (Quantitative Structure Activity Relationship) modeling approach uses linear regressions or regression trees of compound structures to determine suitability. The software of the Chemical Computing Group, Inc. (Montreal, Canada) uses high-throughput screening experimental data on active as well as inactive compounds, to create a probabilistic QSAR model, which is subsequently used to select lead compounds. The Binary QSAR method is based upon three characteristic properties of compounds that form a “descriptor” of the likelihood that a particular compound will or will not perform a required function: partial charge, molar refractivity (bonding interactions), and logP (lipophilicity of molecule). Each atom has a surface area, in the molecule and it has these three properties associated with it. All atoms of a compound having a partial charge in a certain range are determined and the surface areas (Van der Walls Surface Area descriptor) are summed. The binary QSAR models are then used to make activity models or ADMET models, which are used to build a combinatorial library. Accordingly, lead compounds identified in initial screens, can be used to expand the list of compounds being screened to thereby identify highly active compounds.


Assays to Identify and Isolate Therapeutic and Prophylactic Compounds

Any assay described herein for identifying binding activity to CD40L and/or an interaction between CD40L and CD40 and/or a functionality of CD40L signaling such as CD40L-induced expression of CD86 and/or CD40L-mediated T cell proliferation and/or CD40L-dependent and CD40-mediated signaling, may be employed to identify a peptidyl or non-peptidyl inhibitor of the present invention. Alternatively, or in addition, one or more accepted animal models of CD40L-dependent signaling (i.e., CD40-mediated event and/or CD40L-dependent CD40-mediated event) may be employed e.g., a murine model of acute airways inflammation, a primate model of allograft rejection, a murine model of diabetes, a murine model of atherosclerosis, a murine model of angiogenesis, a murine EAE model of multiple sclerosis, a rodent model of graft-versus-host-disease, a rodent model of mercuric chloride-induced glomerulonephritis, and a rodent model of inflammatory bowel disease, each of which is known to the skilled artisan. For example, screens for inhibition of CD40L are described herein which can distinguish between peptide inhibitors with distinct modes of action. In one example, competitive inhibitors of the interaction between CD40L and its cognate receptor CD40 are identified. In another example, allosteric inhibitors which alter the conformation of CD40L upon binding, thereby blocking its activity, are identified.


For example, a compound library or mixture may be screened e.g., to isolate a compound that antagonizes the interaction between CD40L and CD40 and/or antagonizes CD40L signaling or CD40 signaling or CD40L-induced expression of CD86 or inhibits CD40L-mediated T cell proliferation. This may require repeated screening of pools of compounds in vitro or in vivo to eventually purify the compound free or substantially free of contaminants.


Alternatively, a previously-isolated compound not known to have the ability to antagonize the interaction between CD40L and CD40 and/or antagonize CD40L signaling or CD40 signaling or CD40L-induced expression of CD86 or to inhibit CD40L-mediated T cell proliferation, is screened by one or more of the foregoing assays to determine whether or not it has the required property.


It is to be understood that the foregoing assays can be utilized in separately or collectively and in any order determined empirically to identify or isolate the desired product at a level of purity and having a suitable activity ascribed to it e.g., for therapy.


The activity and purity of the compounds determined by these assays make the compound suitable of formulations e.g., injectable and/or inhalable medicaments and/or oral formulations for treatment and/or prophylaxis.


The present invention encompasses the use of any in silico or in vitro analytical method and/or industrial process for carrying the screening methods described herein into a pilot scale production or industrial scale production of a compound identified in such screens. This invention also provides information for such production method(s). Accordingly, the present invention also provides a process for identifying or determining a compound supra, said method comprising:

  • (i) performing a method as described herein according to any embodiment to thereby identify a compound;
  • (ii) optionally, determining the amount of the compound;
  • (iii) optionally, determining the structure of the compound; and
  • (iv) providing the compound or the name or structure of the compound such as, for example, in a paper form, machine-readable form, or computer-readable form.


As used herein, the term “providing the compound” shall be taken to include any chemical or recombinant synthetic means for producing said compound (with or without derivitization) or alternatively, the provision of a compound that has been previously synthesized by any person or means.


In one example, the compound or the name or structure of the compound is provided with an indication as to its use e.g., as determined by a screen described herein.


The present invention additionally provides a process for identifying or determining a compound or modulator supra, said method comprising:

  • (i) performing a method as described herein according to any embodiment to thereby identify or determine a compound;
  • (ii) optionally, determining the amount of the compound;
  • (iii) optionally, determining the structure of the compound;
  • (iv) optionally, providing the name or structure of the compound such as, for example, in a paper form, machine-readable form, or computer-readable form; and
  • (v) providing the compound.


In the case of a peptide, the method optionally further comprises providing a chemical derivative of the peptide by protection of the amino-or carboxyl-terminus, cyclization of the peptide or construction of the peptide as a retroinverso peptide. The method also optionally involves identifying and/or validating one or more peptidyl compounds such as by displaying a peptide in vitro or on a bacteriophage particle, e.g., using lytic T7-based or non-lytic M13-based phage display, identifying the sequence of the peptide, making the compound by recombinant means or peptide chemistry, and testing the ability of the peptide to produce a desired effect such as reduced or prevented neutrophilic inflammation or inhibition of a specific protein interaction involved in a neutrophilic inflammatory response. Preferably, the peptide is displayed within a protein-based scaffold e.g., a scaffold structure derived from lipocalin, ankyrin repeats, fibronectin, kunitz domains, A-domains, affibodies etc. Alternatively the inhibitory peptide can be grafted into such a protein based scaffold in order to enhance stability or improve stability.


In one example, the compound or the name or structure of the compound is provided with an indication as to its use e.g., as determined by a screen described herein.


The present invention also provides a method of manufacturing a compound identified by a screening method described herein according to any embodiment for use in medicine comprising:

    • (i) performing a method as described herein according to any embodiment to thereby identify or determine a compound; and
    • (ii) using the compound in the manufacture of a therapeutic for use in medicine.


In one example, the method comprises the additional step of isolating the compound. Alternatively, a compound is identified and is produced for use in the manufacture of a compound for use in medicine.


Formulations

The present invention provides for the use of an inhibitor of the present invention as described according to any example hereof in the preparation of a medicament for treatment of a subject in need thereof e.g., for attenuation or alleviation or amelioration of an inappropriate or adverse humoral immune response, such as an immune response associated with or causative of an autoimmune disease. Alternatively, or in addition, the invention provides for use of an inhibitor of the present invention as described according to any example hereof in the preparation of a medicament for treatment for preventing or reducing an immune response against an antigen having a therapeutic or adaptive benefit to a subject. Alternatively, or in addition, the invention provides for use of an inhibitor of the present invention as described according to any example hereof in the preparation of a medicament for preventing or reducing a counter-adaptive immune response in a subject.


A compound of the invention as described herein according to any embodiment is formulated for therapy or prophylaxis with a carrier or excipient e.g., suitable for inhalation or injection.


The term “carrier or excipient” as used herein, refers to a carrier or excipient that is conventionally used in the art to facilitate the storage, administration, and/or the biological activity of an active compound. A carrier may also reduce any undesirable side effects of the active compound. A suitable carrier is, for example, stable, e.g., incapable of reacting with other ingredients in the formulation. In one example, the carrier does not produce significant local or systemic adverse effect in recipients at the dosages and concentrations employed for treatment. Such carriers and excipients are generally known in the art. Suitable carriers for this invention include those conventionally used, e.g., water, saline, aqueous dextrose, dimethyl sulfoxide (DMSO), and glycols are preferred liquid carriers, particularly (when isotonic) for solutions. Suitable pharmaceutical carriers and excipients include starch, cellulose, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, magnesium stearate, sodium stearate, glycerol monostearate, sodium chloride, glycerol, propylene glycol, water, ethanol, one or more alkylsaccharides, and the like.


The skilled artisan will be aware of a suitable carrier or excipient. For example, a, carrier or excipient does not inhibit the anti-inflammatory activity of a CD40L-dependent signaling inhibitor. In one example, the carrier or excipient permits the inhibitor to inhibit or reduce inflammation.


The formulations can be subjected to conventional pharmaceutical expedients, such as sterilization, and can contain a conventional pharmaceutical additive, such as a preservative and/or a stabilizing agent and/or a wetting agent and/or an emulsifying agent and/or a salt for adjusting osmotic pressure and/or a buffer and/or other additives known in the art. Other acceptable components in the composition of the invention include, but are not limited to, isotonicity-modifying agents such as water and/or saline and/or a buffer including phosphate, citrate, succinate, acetic acid, or other organic acids or their salts.


In an example, a formulation includes one or more stabilizers, reducing agents, anti-oxidants and/or anti-oxidant chelating agents. The use of buffers, stabilizers, reducing agents, anti-oxidants and chelating agents in the preparation of compositions, is known in the art and described, for example, in Wang et al. J. Parent. Drug Assn. 34:452-462, 1980; Wang et al. J. Parent. Sci. Tech. 42:S4-S26 (Supplement), 1988. Suitable buffers include acetate, adipate, benzoate, citrate, lactate, maleate, phosphate, tartarate, borate, tri(hydroxymethyl aminomethane), succinate, glycine, histidine, the salts of various amino acids, or the like, or combinations thereof. Suitable salts and isotonicifiers include sodium chloride, dextrose, mannitol, sucrose, trehalose, or the like. Where the carrier is a liquid, it is preferred that the carrier is hypotonic or isotonic with oral, conjunctival, or dermal fluids and has a pH within the range of 4.5-8.5.


Where the carrier is in powdered form, it is preferred that the carrier is also within an acceptable non-toxic pH range.


In another example, a formulation as described herein according to any embodiment additionally comprises a compound that enhances or facilitates uptake of a compound. Suitable dermal permeation enhancers are, for example, a lipid disrupting agent (LDA), a solubility enhancer, or a surfactant. LDAs are typically fatty acid-like molecules proposed to fluidize lipids in the human skin membrane. Suitable LDAs are described, for example, in Francoeur et al., Pharm. Res., 7: 621-627, 1990 and U.S. Pat. No. 5,503,843. For example, a suitable LDA is a long hydrocarbon chain with a cis-unsaturated carbon-carbon double bond. These molecules have been shown to increase the fluidity of the lipids, thereby increasing drug transport. For example, oleic acid, oleyl alcohol, decanoic acid, and butene diol are useful LDAs.


Solubility enhancers act by increasing the maximum concentration of drug in a composition, thus creating a larger concentration gradient for diffusion. For example, a lipophilic vehicle isopropyl myristate (IPM) or an organic solvent ethanol or N-methyl pyrrolidone (NMP) or dimethyl sulfoxide (DMSO) are suitable solubility enhancers (Liu et al., Pharm. Res. 8: 938-944, 1991; and Yoneto et al., J. Pharm. Sci. 84: 853-860, 1995).


Surfactants are amphiphilic molecules capable of interacting with the polar and lipid groups in the skin. These molecules have affinity to both hydrophilic and hydrophobic groups, which facilitate in traversing complex regions of the dermis. Suitable surfactants include, for example, an anionic surfactant lauryl sulfate (SDS) or a nonionic surfactant polysorbate 80 (Tween 80). Suitable surfactants are described, for example, in Sarpotdar et al., J. Pharm. Sci. 75: 176-181, 1986)


In another example, the formulation is a microemulsion. Microemulsion systems are useful for enhancing transdermal delivery of a compound. Characteristics of such microemulsion systems are sub-micron droplet size, thermodynamic stability, optical transparency, and solubility of both hydrophilic and hydrophobic components. Microemulsion systems have been shown to be useful for transdermal delivery of compounds and to exhibit improved solubility of hydrophobic drugs as well as sustained release profiles (Lawrence, et. al. Int. Journal of Pharmaceutics 111: 63-72, 1998).


In another example, a formulation comprises a peptidyl moiety conjugated to a hydrolysable polyethylene glycol (PEG) essentially as described by Tsubery et al., J. Biol. Chem. 279 (37) pp. 38118-38124. Alternatively, the formulation comprises a peptidyl moiety conjugated to hydroxyethyl starch (HES) or polyglycine or serum protein moiety or serum protein-binding moiety. Without being bound by any theory or mode of action, such formulations provide for extended or longer half-life of the peptide moiety in circulation.


In another example, a formulation comprises a nanoparticle comprising the peptide moiety or other active ingredient bound to it or encapsulated within it. Without being bound by any theory or mode of action, delivery of a peptidyl composition from a nanoparticle may reduce renal clearance of the peptide(s).


In another example, a formulation comprises a liposome carrier or excipient to facilitate uptake of an inhibitor into a cell. Liposomes are considered to interact with a cell by stable absorption, endocytosis, lipid transfer, and/or fusion (Egerdie et al., J. Urol. 142:390, 1989). For example, liposomes comprise molecular films, which fuse with cells and provide optimal conditions for wound healing (K. Reimer et al., Dermatology 195(suppl. 2):93, 1999). Generally, liposomes have low antigenicity and can be used to encapsulate and deliver components that cause undesirable immune responses in patients (Natsume et al., Jpn. J. Cancer Res. 91:363-367, 2000)


For example, anionic or neutral liposomes often possess excellent colloidal stability, since substantially no aggregation occurs between the carrier and the environment. Consequently their biodistribution is excellent, and their potential for irritation and cytotoxicity is low.


Alternatively, cationic liposomal systems, e.g. as described in Mauer et al., Molecular Membrane Biology, 16: 129-140, 1999 or Maeidan et al., BBA 1464: 251-261, 2000 are useful for delivering compounds into a cell. Such cationic systems provide high loading efficiencies. Moreover, PEGylated cationic liposomes show enhanced circulation times in vivo (Semple BBA 1510, 152-166, 2001).


Amphoteric liposomes are a recently described class of liposomes having an anionic or neutral charge at pH 7.4 and a cationic charge at pH 4. Examples of these liposomes are described, for example, in WO 02/066490, WO 02/066012 and WO 03/070735. Amphoteric liposomes have been found to have a good biodistribution and to be well tolerated in animals and they can encapsulate nucleic acid molecules with high efficiency.


USSN09/738,046 and U.S. Ser. No. 10/218,797 describe liposomes suitable for the delivery of peptides or proteins into a cell.


Injectable Formulations

Injectable formulations comprising peptide(s) of the invention or other active ingredient and a suitable carrier or excipient preferably have improved stability and/or rapid onset of action, and are for intravenous, subcutaneous, intradermal or intramuscular injection.


For parenteral administration, the peptidyl component or other active ingredient, may be administered as injectable doses of a solution or suspension in a physiologically acceptable diluent with a pharmaceutical carrier which can be a sterile liquid such as water or oil e.g., petroleum, animal, vegetable or synthetic oil including any one or more of peanut oil, soybean oil, mineral oil, etc. Surfactant and other pharmaceutically acceptable adjuvants or excipients may be included. In general, water, saline, aqueous dextrose or other related sugar solution, ethanol or glycol e.g., polyethylene glycol or propylene glycol, is a preferred carrier.


The injectable formulations may also contain a chelator e.g., EDTA, and/or a dissolution agent e.g., citric acid. Such components may assist rapid absorption of the active ingredient into the blood stream when administered by injection.


One or more solubilizing agents may be included in the formulation to promote dissolution in aqueous media. Suitable solubilizing agents include e.g., wetting agents such as polysorbates, glycerin, a poloxamer, non-ionic surfactant, ionic surfactant, food acid, food base e.g., sodium bicarbonate, or an alcohol. Buffer salts may also be included for pH control.


Stabilizers are used to inhibit or retard drug decomposition reactions in storage or in vivo which include, by way of example, oxidative reactions, hydrolysis and proteolysis. A number of stabilizers may be used e.g., protease inhibitors, polysaccharides such as cellulose and cellulose derivatives, and simple alcohols, such as glycerol; bacteriostatic agents such as phenol, m-cresol and methylparaben; isotonic agents, such as sodium chloride, glycerol, and glucose; lecithins, such as example natural lecithins (e.g. egg yolk lecithin or soya bean lecithin) and synthetic or semisynthetic lecithins (e.g. dimyristoylphosphatidylcholine, dipalmitoylphosphatidylcholine or distearoyl-phosphatidylcholine; phosphatidic acids; phosphatidylethanolamines; phosphatidylserines such as distearoyl-phosphatidylserine, dipalmitoylphosphatidylserine and diarachidoylphospahtidylserine; phosphatidylglycerols; phosphatidylinositols; cardiolipins; sphingomyelins. In one example, the stabilizer may be a combination of glycerol, bacteriostatic agents and isotonic agents.


In one example, the peptidyl component or other active ingredient of an injectable formulation is provided as a dry powder in a sterile vial or ampoule. This is mixed with a pharmaceutically acceptable carrier, excipient, and other components of the formulation shortly before or at the time of administration. Such an injectable formulation is produced by mixing components such as a carrier and/or excipient e.g., saline and/or glycerol and/or dissolution agent and/or chelator etc to form a solution to produce a “diluent”, and then and sterilizing the diluent e.g., by heat or filtration. The peptidyl component or other active agent is added separately to sterile water to form a solution, sterile-filtered, and a designated amount is placed into each of a number of separate sterile injection bottles. The peptide or other active agent solution is then lyophilized to form a powder and stored e.g., separately from the diluent to retain its stability. Prior to administration, the diluent is added to the injection bottle containing the dried peptidyl component or other active agent. After the predetermined amount of formulation is injected into the patient, the remaining solution may be stored, e.g., frozen or refrigerated.


In another example, the formulation is prepared as a frozen mixture ready for use upon thawing. For example, the peptidyl component or other active agent is combined with the diluent, sterile filtered into multi-use injection bottles or ampoules and frozen prior to use.


Intranasal Formulations

For intranasal administration, powdery preparations having improved absorbability have been proposed. They are prepared e.g., by adsorbing physiologically active linear peptides onto a polyvalent metal compound such as hydroxyapatite or calcium carbonate (e.g., EP 0 681 833 A2). Peptides can be cyclized to improve their stability and resistance to peptidases in the nasal mucosa e.g., by synthesis as a continuous cyclotide or by oxidation of flanking cysteine residues. Alternatively, peptides may be stabilized in a particular conformation by means of artificially ‘stapling’ using chemical linkers e.g., Walensky et al., Science 305, 1466-1470 (2004).


Preferably, the peptide is dispersed homogeneously in and adsorbed homogeneously onto a physiologically acceptable particulate carrier, which can be a physiologically acceptable powdery or crystalline polyvalent metal carrier and/or organic carrier, whose mean particle size is in the range of 20 to 500 microns. In a preferred form, the peptidyl inhibitor according to any example hereof is formulated for intranasal delivery an alkyl-saccharide transmucosal delivery-enhancing excipient such as Intraveil (Aegis Therapeutics).


Suitable polyvalent metal component of the carrier include physiologically acceptable metal compounds having more than 2 valency, and may include, for example, aluminum compounds, calcium compounds, magnesium compounds, silicon compounds, iron compounds and zinc compounds. Such metal compounds are commonly used as excipients, stabilizers, filing agents, disintegrants, lubricants, adsorbents and coating agents for medical preparations.


Preferred aluminum compounds include, for example, dry aluminum hydroxy gel, aluminum hydroxychloride, synthetic aluminum silicate, light aluminum oxide, colloidal aluminum silicate hydrate, aluminum magnesium hydroxide, aluminum hydroxide, aluminum hydroxide gel, aluminum sulfate, dihydroxyaluminum aminoacetate, aluminum stearate, natural aluminum silicate, aluminum monostearate and potassium aluminum sulfate. Among them, the preferable aluminum compound is aluminum hydroxide.


Preferred calcium compounds include, for example, apatite, hydroxyapatite, calcium carbonate, calcium disodium EDTA, calcium chloride, calcium citrate, calcium glycerophosphate, calcium gluconate, calcium silicate, calcium oxide, calcium hydroxide, calcium stearate, calcium phosphate tribasic, calcium lactate, calcium pantothenate, calcium oleate, calcium palmitate, calcium D-pantothenate, calcium alginate, calcium phosphate anhydride, calcium hydrogenphosphate, calcium primary phosphate, calcium acetate, calcium saccharate, calcium sulfate, calcium secondary phosphate, calcium para-aminosalicylate and bio-calcilutite compounds. Bio-calcilutite compounds such as crystalline calcium pyrophosphate, calcium secondary phosphate, octacalcium phosphate, tricalcium phosphate and crystalline calcium oxalate are analogous to hydroxyapatite and may also be used as a physiologically acceptable powdery or crystalline carrier. Preferable calcium compounds are hydroxyapatite, calcium carbonate or calcium lactate.


Preferred magnesium compound components of the physiologically acceptable powdery or crystalline carrier include, for example, magnesium L-aspartate, magnesium chloride, magnesium gluconate, magnesium aluminate silicate, magnesium silicate, magnesium oxide, magnesium hydroxide, magnesium stearate, magnesium carbonate, magnesium aluminate metasilicate, magnesium sulfate, sodium magnesium silicate and synthetic sodium magnesium silicate. Among them, preferable magnesium compound is magnesium stearate.


Other metal compounds with more than 2 valency may be silicon compounds such as silicon oxide hydrate, light silicic anhydride, synthetic hydrotalcite, diatomaceous earth and silicon dioxide; iron compounds such as ferrous sulfate; and zinc compounds such as zinc chloride, zinc stearate and zinc sulfate.


Particulate organic carriers may be a fine powder from grain, preferably of rice, wheat, buckwheat, barley, soybean, corn, millet, foxtail millet and the like.


Such formulations may optionally comprise an absorption enhancer. Preferred absorption enhancers which may be one of the components of the nasally administrable composition is a pharmaceutically acceptable natural (e.g. cellulose, starch and their derivatives) or unnatural polymer material. A preferred embodiment of the cellulose and its derivatives is microcrystalline cellulose, methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, hydroxypropylmethyl cellulose phthalate, cellulose acetate, cellulose acetate phthalate, carboxymethyl cellulose, low carboxymethyl cellulose sodium, carboxymethylethyl cellulose and the like. A preferable embodiment of the starch and its derivatives is corn starch, potato starch, rice starch, glutinous rice starch, wheat starch, pregelatinized starch, dextrin, sodium carboxymethyl starch, hydroxypropyl starch, pullulan and the like. Other natural polymers such as agar, sodium alginate, chitin, chitosan, egg yolk lecithin, gum arabic, tragacanth, gelatine, collagen, casein, albumin, fibrinogen, and fibrin may also be used as absorption enhancer. A preferable embodiment of the unnatural polymer is sodium polyacrylate, polyvinyl pyrrolidone, and the like. Preferred absorption enhancers are fine powder of rice, glutinous rice, starch, gelatine, dextrin, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, polyvinyl pyrrolidone, egg yolk lecithin, gum arabic, tragacanth or a mixture thereof. More preferable absorption enhancers are fine powder of glutinous rice, starch, gelatine, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, polyvinyl pyrrolidone, tragacanth or a mixture thereof.


Even more preferable absorption enhancers are fine powder of glutinous rice or hydroxypropyl cellulose. Most preferable absorption enhancer is fine powder of glutinous rice. The mean particle size of the absorption enhancer is preferably not more than 250 microns, more preferably from 20 to 180 microns.


The above absorption enhancers may be used alone or in combination of two or more absorption enhancers in the physiologically acceptable powdery or crystalline carrier.


Water-soluble carriers are preferred to increase adsorption of the active substance in the nasal mucosa. Alternatively, this is achieved by homogeneous dispersion of the active substance in a water-insoluble carrier e.g., hydroxyapatite, calcium carbonate, calcium lactate, aluminum hydroxide or magnesium stearate, preferably in the presence of an absorption enhancer, and homogeneously adsorbing the active substance there onto.


Calcium carbonate, calcium lactate, aluminum hydroxide or magnesium stearate is usually used as a stabilizer, lubricant, agent to add lustre, excipient, dispersing agent or coating agent for a pharmaceutical preparation; however, it has been found that these compounds having a mean particle size of not more than 500 microns can be used as a carrier for the intranasal formulations, and promote absorption of a physiologically active substances into the body by nasal administration.


Additional Components

In another example of the invention, a formulation comprises an additional component or compound e.g., a cytokine or growth factor, such as, for example, an interleukin e.g., IL-2 or IL-8, IL-10, IL-13 or IL-17 and/or an interferon molecule e.g., IFN-α or IFN-β or IFN-γ, or IFN-λ and/or transforming growth factor β and/or platelet derived growth factor and/or nerve growth factor and/or heparin binding epidermal growth factor and/or epidermal growth factor and/or keratinocyte growth factor and/or platelet derived activating factor and/or platelet derived epithelial growth factor and/or a fibroblast growth factor an/or a keratinocyte growth factor. For example, Puolalckainen et al., J. Surg. Res., 58: 321-329, 1995 describe formulations comprising transforming growth factor β; compositions comprising platelet derived growth factor have been described by Lepisto et al., J. Surg. Res., 53: 596-601, 1992; formulations comprising fibroblast growth factor are described, for example, in Brown et al., Surg., 121: 372-380, 1997; formulations comprising nerve growth factor are described in, for example, Matsuda et al., J. Exp. Med., 187: 297-306, 1998.


Modes of Administration

The present invention contemplates any mode of administration of a medicament or formulation as described herein, however one or a plurality of intranasal and/or injected and/or oral doses is preferred. Combinations of different administration routes are also encompassed e.g., intranasal and/or intravenous and/or oral.


Compositions according to the present invention are administered in an aqueous solution as a nasal or pulmonary spray and may be dispensed in spray form by a variety of methods known to those skilled in the art. Preferred systems for dispensing liquids as a nasal spray are disclosed in U.S. Pat. No. 4,511,069. Such formulations may be conveniently prepared by dissolving compositions according to the present invention in water to produce an aqueous solution, and rendering the solution sterile. The formulations may be presented in multi-dose containers, for example in the sealed dispensing system disclosed in U.S. Pat. No. 4,511,069. Other suitable nasal spray delivery systems have been described in Transdermal Systemic Medication, Y. W. Chien Ed., Elsevier Publishers, New York, 1985; and in U.S. Pat. No. 4,778,810 (each incorporated herein by reference). Additional aerosol delivery forms may include, e.g., compressed air-, jet-, ultrasonic-, and piezoelectric nebulizers, which deliver the biologically active agent dissolved or suspended in a pharmaceutical solvent, e.g., water, ethanol, or a mixture thereof.


Nasal and pulmonary spray solutions of the present invention typically comprise the drug or drug to be delivered, optionally formulated with a surface active agent, such as a nonionic surfactant (e.g., polysorbate-80), and one or more buffers. In some embodiments of the present invention, the nasal spray solution further comprises a propellant. The pH of the nasal spray solution is optionally between about pH 6.8 and 7.2, but when desired the pH is adjusted to optimize delivery of a charged macromolecular species (e.g., a therapeutic protein or peptide) in a substantially non-ionized state. The pharmaceutical solvents employed can also be a slightly acidic aqueous buffer (pH 4-6). Suitable buffers for use within these compositions are as described above or as otherwise known in the art. Other components may be added to enhance or maintain chemical stability, including preservatives, surfactants, dispersants, or gases. Suitable preservatives include, but are not limited to, phenol, methyl paraben, paraben, m-cresol, thiomersal, benzylalkonimum chloride, and the like. Suitable surfactants include, but are not limited to, oleic acid, sorbitan trioleate, polysorbates, lecithin, phosphotidyl cholines, and various long chain diglycerides and phospholipids. Suitable dispersants include, but are not limited to, ethylenediaminetetraacetic acid, and the like. Suitable gases include, but are not limited to, nitrogen, helium, chlorofluorocarbons (CFCs), hydrofluorocarbons (HFCs), carbon dioxide, air, and the like.


Within alternate embodiments, mucosal formulations are administered as dry powder formulations comprising the biologically active agent in a dry, usually lyophilized, form of an appropriate particle size, or within an appropriate particle size range, for intranasal delivery. Minimum particle size appropriate for deposition within the nasal or pulmonary passages is often about 0.5 micron mass median equivalent aerodynamic diameter (MMEAD), commonly about 1 micron MMEAD, and more typically about 2 micron MMEAD. Maximum particle size appropriate for deposition within the nasal passages is often about 10 micron MMEAD, commonly about 8 micron MMEAD, and more typically about 4 micron MMEAD. Intranasally respirable powders within these size ranges can be produced by a variety of conventional techniques, such as jet milling, spray drying, solvent precipitation, supercritical fluid condensation, and the like. These dry powders of appropriate MMEAD can be administered to a patient via a conventional dry powder inhaler (DPI) which rely on the patient's breath, upon pulmonary or nasal inhalation, to disperse the power into an aerosolized amount. Alternatively, the dry powder may be administered via air assisted devices that use an external power source to disperse the powder into an aerosolized amount, e.g., a piston pump.


Dry powder devices typically require a powder mass in the range from about 1 mg to 20 mg to produce a single aerosolized dose (“puff”). If the required or desired dose of the biologically active agent is lower than this amount, the powdered active agent will typically be combined with a pharmaceutical dry bulking powder to provide the required total powder mass. Preferred dry bulking powders include sucrose, lactose, dextrose, mannitol, glycine, trehalose, human serum albumin (HSA), starch e.g., hydroxyethyl starch (HES). Other suitable dry bulking powders include cellobiose, dextrans, maltotriose, pectin, sodium citrate, sodium ascorbate, and the like.


Standard methods are used to administer injectable formulations of the present invention.


Medical Indications

The invention can be used for treatment or prophylaxis of any mammalian subject in need of, or already receiving, therapy for one or more consequences of aberrant or inappropriate CD40L-depdenent signaling, including CD40L-dependent CD40-mediated signaling or CD40L-mediated signaling.


In one example, an inhibitor of the present invention as described according to any example hereof is for treatment or therapy of a subject in need thereof e.g., for attenuation or alleviation or amelioration of an inappropriate or adverse humoral immune response, such as an immune response associated with or causative of an autoimmune disease. Alternatively, or in addition, an inhibitor of the present invention as described according to any example hereof is for preventing or reducing an immune response against an antigen having a therapeutic or adaptive benefit to a subject. Alternatively, or in addition, an inhibitor of the present invention as described according to any example hereof is for use in a method to prevent or reduce a counter-adaptive immune response in a subject.


As used herein, the term “treatment” or “therapy” means to improve a subject's clinical state e.g., by reducing, alleviating, ameliorating or preventing one or more adverse indications of a disease, condition or syndrome. The treatment or therapy may involve complete abrogation of adverse indication(s) or comprise a partial improvement therein.


In another example, an inhibitor of the present invention as described according to any example hereof is for therapy of an autoimmune disease.


As used herein, “autoimmune disease” describes a disease state or syndrome whereby a subject's body produces a dysfunctional immune response against the subject's own body components, with adverse effects. This may include production of B cells producing antibodies with specificity for all antigens, allergens or major histocompatibility (MHC) antigens, or it may include production of T cells bearing receptors that recognize self-components and produce cytokines that cause inflammation. Examples of autoimmune diseases include, but are not limited to, ulcerative colitis, Crohn's disease, multiple sclerosis, rheumatoid arthritis, diabetes mellitus, pernicious anemia, autoimmune gastritis, psoriasis, Bechet's disease, Wegener's granulomatosis, Sarcoidois, autoimmune thyroiditis, autoimmune oophoritis, bullous pemphigoid, phemphigus, polyendocrinopathies, Still's disease, Lambert-Eaton myasthenia syndrome, myasthenia gravis, Goodpasture's syndrome, autoimmune orchitis, autoimmune uveitis, systemic lupus erythematosus, multiple sclerosis, Sjogren's Syndrome and ankylosing spondylitis.


In one example, the autoimmune disease is rheumatoid arthritis e.g., wherein the inhibitor reduces or prevents CD40L-dependent induction of anti-collagen antibodies. In another example, the autoimmune disease is type I diabetes. In another example, the autoimmune disease is systemic lupus erythematosus (SLE) e.g., wherein the inhibitor reduces or prevents CD40L-dependent anti-dsDNA and anti-nucleosomal autoantibody production. In another example, the autoimmune disease is multiple sclerosis e.g., wherein the inhibitor reduces or prevents CD40L-dependent anti-myelin autoantibody production.


In another example, an inhibitor of the present invention as described according to any example hereof is for therapy of inflammation e.g., inflammation associated with an autoimmune disease, periodic fever syndrome, sepsis or acute respiratory distress syndrome.


In another example, an inhibitor of the present invention as described according to any example hereof is for therapy of idiopathic thrombocytopenic purpura (ITP).


In another example, an inhibitor of the present invention as described according to any example hereof is for therapy of irritable bowel syndrome/disease (IBD).


In another example, an inhibitor of the present invention as described according to any example hereof is for therapy of one or more CD40L-dependent vascular complication of diabetes e.g., cardiac ischemia, atherosclerosis.


In another example, a peptidyl inhibitor of the present invention as described according to any example hereof is for reducing the risk of complication of transplantation by blocking a CD40L regulated process involved in graft rejection.


In another example, an inhibitor of the present invention as described according to any example hereof attenuates or ameliorates bio-inhibitory humoral immunity against one or more therapeutic proteins e.g., thrombin, clotting factor such as factor VIII, one or more cytokines e.g., an interleukin (IL) such as IL-2 or an interferon (IFN) such as IFN-α, IFN-β, IFN-γ or IFN-λ. For example, in the therapy of hemophilia, subjects at risk of developing humoral immunity to factor VIII may be treated with an inhibitor of the present invention. Similarly, subjects suffering from an autoimmune disease e.g., multiple sclerosis, or a viral infection e.g., HCV infection and receiving interferon therapy may be treated with an inhibitor of the present invention. Procedures for determining whether a subject has developed an inhibitory response against a therapeutic peptide are well known e.g., Hematology: Clinical and Laboratory Practice, vol. 2, Bick, ed., Mosby-Year Book, Inc., publ. (1993), pp. 1544-1548.


The subject will be a mammalian animal, e.g., a human or non-human animal, such as a domesticated non-human mammal, including a companion or laboratory mammal, e.g., selected from chimpanzees, monkeys, sheep, horses, cattle, goats, pigs, dogs, cats, rabbits, guinea pigs, hamsters, gerbils, rats and mice. For example, the subject may be a subject afflicted with, or at risk of, developing an autoimmune disease or other CD40L-dependent condition such as an exogenous protein inhibitor syndrome.


The present invention is described further in the following non-limiting examples:


Example 1
Isolation and Characterization of Representative Peptidyl Inhibitors

This example provides representative peptidyl inhibitors of the present invention and describes methods for their isolation and validation. Additional peptidyl inhibitors of the invention are provided in the basic application, incorporated herein by reference.


Peptide Display

Using phage display, expression libraries produced from fragments of prokaryote and eukaryote compact genomes, including Salmonella enterica, Bacillus subtilis, Listeria innocua, Neisseria meningitidis, Escherichia coli, Thermotoga maritima, Sulfolobus solfataricus, Borrelia burgdorferi, Deinococcus radiodurans, Campylobacter jejuni, Geobacter sulfurreducens, Pseudomonas aeruginosa, Bordetella pertussis, Haloarcula marismortui and Chlorobium tepidum. The genome fragments inserted into the phage display libraries encode peptides, including natural open reading frames, capable of forming secondary structures or super-secondary structures (including folds, domains and sub-domains of proteins). Because the fragments are derived from prokaryotes or non-mammalian eukaryotes having compact genomes, the encoded peptides do not bind CD40L in their native context.


Peptides were displayed in trans with the p3 protein from the vector pJuFo. Alternatively, the peptides were displayed as fusion proteins with the p3 protein, wherein nucleic acid encoding the peptidyl inhibitor was positioned downstream of a PelB leader sequence and upstream of a sequence encoding an HA-p3 fusion moiety, and wherein the vector was configured such that the proportion of natural open reading frames displayed in the PelB-peptidyl inhibitor-HA-p3 fusion peptide is enhanced.


Base Peptides

In primary biopanning with CD40L, the inventors identified peptides that bind to CD40L. Table 2 provides the sequences of representative base peptides i.e., SEQ ID Nos: 1-18.


Modification of Base Peptides by Removal of Internal Cysteine Residues

The sequences of the base peptides having internal or C-terminal cysteine residues were modified by synthesizing the peptides with a serine residues in place of the cysteine. The sequences of the derivative peptides are provided in Table 3 i.e., SEQ ID Nos: 19-34.


Chiral Analogs of Base Peptides and Serine Derivatives

The inventors have also produced retro-inverted analogs of the base peptides comprising e.g., two or more retro-inverted amino acids and preferably, comprising a reversed amino acid sequence wherein all amino other than glycine (which is not chiral) are D-amino acids. The amino acid sequences of representative chiral analogs are set forth in Table 4 i.e., SEQ ID Nos: 35-43.


Modification of Base Peptides by Directed Evolution

The inventors have also produced multimeric forms of the peptides comprising one or two or three CD40L peptide ligands of the invention. In one example, a recombinase-based system of the present invention is used to produce the multimeric peptides. In another example, cysteine-containing linkers have been added to the peptides as described in the preceding paragraph and the peptides have been dimerized by chemical oxidation of the sulfhydryl group on the terminal cysteine residue e.g., using aldrithiol-2. Homodimers and heterodimers have been produced and assayed by chemiluminescent proximity assay for their ability to inhibit CD40L-CD40 interaction.


In one example, homodimers and heterodimers comprising the peptide CD40LM16 were produced, including heterodimers comprising the peptide CD40LM16 and a peptide selected from CD40LM14, CD40LM19, and CD40LM7189.


In another example, peptide monomers are separated by tetra-glycine spacer or linker. A representative sequence of a heterodimeric peptide is presented in Table 2 (SEQ ID No: 44), which comprises the CD40L M1206 monomer fused to a second peptidyl inhibitory monomer via a tetra-glycine spacer.


Modification of Base Peptides by Addition of PEG Moiety

The inventors have also produced, or will produce, derivatives of the peptides and analogs set forth in Tables 2 to 4 (SEQ ID Nos: 1-44) comprising polyethylene glycol or “PEG” (i.e., PEGylated peptides) added to their N-termini.


The N-terminal PEG residues may be separated from the peptidyl inhibitor by a linker or spacer. The linker or spacer may be e.g., an 8-amino-3,6-dioxaoctanoyl linker, an 8-amino-3,6-dioxaoctanoyl cysteine spacer, an 8-amino-3,6-dioxaoctanoyl lysine spacer, an 8-amino-3,6-dioxaoctanoyl lysyl cysteine spacer, or 8-amino-3,6-dioxaoctanoyl lysyl-lysyl-cysteine spacer. Exemplary spacers lined to the N-terminus of a peptide are represented schematically in FIGS. 1a to 1c. Exemplary peptide dimers separated by spacers are represented schematically in FIGS. 1d and 1e.


Exemplary PEGylated peptides are set forth in Tables 2 to 4 hereof i.e., the peptides for which kinetic data are provided.


Competitive Inhibition of Binding to CD40L

In a primary validation assay, the ability of base peptides to bind specifically to CD40L was confirmed by demonstrating that soluble CD40L was able to compete with the peptide displayed by the phage for binding to bound CD40L. In particular, CD40L-binding activity of phage displaying the peptides was inhibited by soluble CD40L with IC50 values of less than 500 nM. Representative data obtained in such assays are presented in FIG. 2 for peptides CD40L M274, CD40L M282, CD40L M283 and CD40L M285. The IC50 values of a sub-set of exemplary peptidyl inhibitors of the invention i.e., peptides CD40L M16, CD40L M19, CD40L M118, and CD40L M150, are also presented in Table 2 hereof.


Determination of Association and Dissociation Constants

Label-free analyses of the associations and dissociations of base peptides and serine derivatives of base peptides with the CD40L target protein were performed using the Octet Red system (ForteBio, Inc., USA) according to the manufacturer's instructions. These analyses provide kinetic constants of CD40L binding activity, especially the association rate constant (Ka) and dissociation rate constant (IQ) of binding, i.e., measures of the “on” and “off” rates of the peptides relative to soluble CD40L.


The equilibrium dissociation constant values (KD) are presented in Tables 2 and 3. Data show that the higher affinity CD40L-binding peptides are generally encoded by natural open reading frames of bacteria or eukaryotes having compact genomes e.g., peptides CD40L M8720, CD40L M8721, CD40L M8747, CD40L M8748, CD40L M9763, CD40L M9780, CD40L M9789 (SEQ ID Nos: 12-18), supporting the hypothesis that protein domains have evolved in these organisms to form stable structures. However, high affinity CD40L-binding peptides were also derived from display in pJuFo e.g., peptides CD40L M16, CD40L M19, CD40L M118 and CD40L M142 (SEQ ID Nos: 1-3 and 5). For those peptides that were modified by substitution of cysteine residues for serine residues, the binding affinity as determined by dissociation constant in the Octet Red assay was generally reduced e.g., compare KD values for peptides in Table 3 to data in Table 2, again supporting the hypothesis that natural open readings represent structures optimized in nature.


Chemiluminescent Proximity Assay

The specificities of the interactions between CD40L and of base peptides or serine derivatives of base peptides or chiral analogs of base peptides, were also demonstrated by chemiluminescent proximity assay employing (i) streptavidin-coated beads; (ii) a biotinylated phospho-CD40L; and (iii) protein A-conjugated beads having bound thereto CD40 receptor (CD40), wherein the biotinylated phospho-CD40L is captured by the streptavidin-coated donor beads via biotin-streptavidin interaction, and then specific peptides and protein A-conjugated CD40 acceptor beads are added. When the CD40L-CD40 interaction occurs, excitation at 680 nm produces an emission at wavelengths in the range of about 520 nm to about 620 nm. If the interaction is inhibited by the peptides, then emission in the 520-620 nm range is reduced or inhibited.


In one example, the Alphascreen assay (PerkinElmer) was employed according to the manufacturer's instructions. Alphascreen data presented herein demonstrate that peptides of the invention have high affinities for CD40L and are able to inhibit CD40-CD40L interactions in the nanomolar concentration range. Representative data obtained in Alphascreen assays are presented in FIGS. 3a-3d hereof for peptides CD40LM16; CD40LM14; CD40LM1_CD40LM19; and CD40LM7189.


Data presented in Tables 2 and 3 and 4 hereof show the EC50 values (i.e., the concentration producing a 50% reduction in control emission at 520-620 nm) for monomeric non-PEGylated (unmodified) and monomeric PEGylated base peptides, serine derivatives and chiral analogs. These data shown that the affinity of binding to CD40L to inhibit the CD40-CD40L interaction is generally enhanced for serine derivative and chiral analogs compared to the base peptides, and that PEGylated chiral analogs may exhibit even higher affinity for CD40L in the inhibition of the CD40-CD40L interaction. Representative data provided in FIG. 4 for peptide CD40L M118 support this conclusion, because the PEGylated chiral analog has a significantly higher affinity of binding than the core peptide or the retroinverted form alone, as evidenced by a reduced EC50 value for the PEGylated chiral analog.


Homodimers and heterodimers were also assayed by chemiluminescent proximity assay for their ability to inhibit CD40L-CD40 interaction. Data shown in Table 5 and FIGS. 5a-5e hereof for exemplary dimeric forms of the CD40L peptide ligands of the invention demonstrate a significantly higher affinity of binding than a corresponding monomeric peptide, in the chemiluminescent proximity assay. Data presented in Table 2 for SEQ ID NO: 44 also demonstrate a 6-fold higher affinity for CD40L than the base monomeric peptide CD40L M1206 which forms the N-terminal moiety of the heterodimer.









TABLE 2 







Base peptidyl inhibitors and kinetic characteristics














IC50

EC50nmodified)
EC50(PEGylated)




nM
KD
CD40L/CD40-Fc/
CD40L/CD40-Fc/


Peptide
Amino acid sequence (SEQ ID NO.)
(nM)
(nM)
BAF617 (nM)
BAF617 (nM)















CD40L M1_6
HPFSIKNVFCIWNFFSVY (SEQ ID NO: 1)
16.3
24
440






CD40L M1_9
PPRYNLFFLFRFYCSFRRDYLYF (SEQ ID NO: 2)
81.1
8
370






CD40L M1_18
LPFVPYRSHVLKYGWFFPVQWSIFAVLPFQYLHRCR
25.0
2.3
350




(SEQ ID NO: 3)









CD40L M1_30
DAAGREFFQIAGLFSFRHHWWQA (SEQ ID NO: 4)









CD40L M1_42
HSFVLFGVNVPFNIIDFQMRVKC (SEQ ID NO: 5)

30
200






CD40L M1_50
PRWVRNRFYCLFVPSGVQRGGIHLWFSNWVR
45.8






(SEQ ID NO: 6)









CD40L M1_82
SIQYHWRYSRFKYYFQLIWVYYCHV (SEQ ID NO: 7)



120





CD40L M2_159
LLYVKVICFFCMLVQYNNFQTYK (SEQ ID NO: 8)


120






CD40L M7_189
LLLFFFSPPFSIFCFSLTTLS (SEQ ID NO: 9)


120






CD40L M7_206
PFTWRPTIFWIIQLIVYMRHF (SEQ ID NO: 10)

280
190
88





CD40L M7_217
LCEMIAIYVFLWKKVFL (SEQ ID NO: 11)









CD40L M8_720
RLPETRKAQAALATKYGIYKFcYYHYWFNGRRILESPVDA

4
238




MLESGEPDFPFMLcWANENWT (SEQ ID NO: 12)









CD40L M8_721
LWRLNEWNYSDAELLSLIEWcIDH (SEQ ID NO: 13)

7
524






CD40L M8_747
LAEHAVWSLKcFPDWEWYNINIFGTDDPNHFWVEcDGHGK

8
197




ILFPGYPEGYYENHFLHSFELED (SEQ ID NO: 13)









CD40L M8_748
RIESLEGEMWLINPFNGETLDEHTLEVWLK

5
184




(SEQ ID NO: 15)









CD40L M9_763
LDLFGDFNGLPEGADRTEFYQHEGHWQNRMILGDSLQVMAS

27





LAEREGLRGKVQcIYFDPPYGIKFN (SEQ ID NO: 16)









CD40L M9_780
LWPESWGGLPPASFFDELDPcINRHLRYPLFSETFTADLPV

12
>1000




GTL (SEQ ID NO: 17)









CD40L M9_789
LLAEQAGTLKSELEAMPLGEYEHAARYVSEVEcNWKTFAGN

4
331




YSEcDHcHANHQDWITDIELEESELEVNDYHWILHYTHDED







VEDEMRIHDEHEAKFYYFWPNFT (SEQ ID NO: 18)









CD40L D1_0014
PFTWRPTIFWIIQLIVYMRHFGGGGSRSELLRENICRYVSL

48





FDHPLQRNTPLDELRFVIFDTETSGFDLVKDRILSIR







(SEQ ID NO: 44)
















TABLE 3 







Derivative peptidyl inhibitors comprising Cys-Ser modification and kinetic characteristic












KD
EC50(PEGylated)




value
CD40L/CD40-Fc/


Peptide
Amino acid sequence (SEQ ID NO.)
(nM)
BAF617 (nM)













CD40L M1_6s
HPFSIKNVFSIWNFFSVY (SEQ ID NO: 19)

48





CD40L M1_9s
PPRYNLFFLFRFYSSFRRDYLYF (SEQ ID NO: 20)
300
48





CD40L M1_18s
LPFVPYRSHVLKYGWFFPVQWSIFAVLPFQYLHRSR (SEQ ID NO: 21)

3,300





CD40L M1_30s
DAAGREFFQIAGLFSFRHHWWQA (SEQ ID NO: 22)
400
32





CD40L M1_42s
HSFVLFGVNVPFNIIDFQMRVKS (SEQ ID NO: 23)
100
112





CD40L M1_50s
PRWVRNRFYSLFVPSGVQRGGIHLWFSNWVR (SEQ ID NO: 24)
600
1500





CD40L M1_82s
SIQYHWRYSRFKYYFQLIWVYYSHV (SEQ ID NO: 25)

120





CD40L M2_159s
LLYVKVISFFSMLVQYNNFQTYK (SEQ ID NO: 26)

120





CD40L M7_189
LLLFFFSPPFSIFSFSLTTLS (SEQ ID NO: 27)

120





CD40L M7_217s
LSEMIAIYVFLWKKVFL (SEQ ID NO: 28)
100






CD40L M8_720s
RLPETRKAQAALATKYGIYGESYYHYWFNGRRILESPVDAMLES





GEPDFPFMLSWANENWT (SEQ ID NO: 29)







CD40L M8_721s
LWRLNEWNYSDAELLSLIEWSIDH (SEQ ID NO: 30)







CD40L M8_747s
LAEHAVWSLKSFPDWEWYNINIFGTDDPNHFWVESDGHGKILFP





GYPEGYYENHFLHSFELED (SEQ ID NO: 31)







CD40L M9_763s
LDLFGDFNGLPEGADRTEFYQHEGHWQNRMILGDSLQVMASLAE





REGLRGKVQSIYFDPPYGIKFN (SEQ ID NO: 32)







CD40L M9_780s
LWPESWGGLPPASFFDELDPSINRHLRYPLFSETFTADLPVGTL





(SEQ ID NO: 33)







CD40L M9_789s
LLAEQAGTLKSELEAMPLGEYEHAARYVSEVESNWKTFAGNYSE





SDHSHANHQDWITDIELEESELEVNDYHWILHYTHDEDVEDEMRI





HDEHEAKFYYFWPNFT (SEQ ID NO: 34)
















TABLE 4 







Representative chiral analogs of base peptidyl inhibitors and kinetic characteristics











EC50(PEGylated)




CD40L/CD40-Fc/


Peptide
Amino acid sequence (SEQ ID NO.)
BAF617 (nM)












CD40L M1_6rd
YVSFFNWISFVNKISFPH (SEQ ID NO: 35)
20





CD40L M1_9rd
FYLYDRRFSSYFRFLFFLNYRPP (SEQ ID NO: 36)
14





CD40L M1_18rd
RCRHLYQFPLVAFISWQVPFFWGYKLVHSRYPVFPL (SEQ ID NO: 37)
688





CD40L M1_30rd
AQWWHHRFSFLGAIQFFERGAAD (SEQ ID NO: 38)
24





CD40L M1_42rd
SKVRMQFDIINFPVNVGFLVFSH (SEQ ID NO: 39)
16





CD40L M1_50rd
RVWNSFWLHIGGRQVGSPVFLSYFRNRVWRP (SEQ ID NO: 40)
568





CD40L M1_82rd
VHSYYVWILQFYYKFRSYRWHYQIS (SEQ ID NO: 41)
10





CD40L M7_206rd
FHRMYVILQIIWFITPRWTFP (SEQ ID NO: 42)
12





CD40L M7_217rd
LFVKKWLFVYIAIMESL (SEQ ID NO: 43)
















TABLE 5







Enhanced inhibition of CD40L-CD40 interaction by dimeric peptides


as determined by Alphascreen proximity assay










Peptide
EC50 (nM)







M1_6S
0.1225 to 0.323



Cys_M1_6S
0.2646



M1_4S
1.616



M1_9S
0.5643



M7_189S
0.1765



Cys_M1_6S homodimer
0.04891



Cys_M1_6S-Cys_M1_4S heterodimer
0.06121



Cys_M1_6S-Cys_M1_9S heterodimer
0.03



Cys_M1_6S-Cys_M1_189S heterodimer
0.0001739







S denotes C-terminal serine;



Cys denotes N-terminal cysteine






Bioassays

To further validate the CD40L peptide antagonists identified by the inventors, bioassays are performed which determine the ability of a peptide of the invention or an analog or derivative thereof to inhibit or antagonize one or more CD40L-dependent biological activities known in the art.


1. Inhibition of Cd86 expression on primary B cells


In one example, the ability of a peptide, analog or derivative of the invention to inhibit or reduce or delay expression of CD86 on primary B-cells was determined in the presence and absence of the peptides, analogs and derivatives of the invention. In particular, CD86 expression was determined by FACS.


Representative data presented in Table 6, and in FIGS. 6a and 6b demonstrate the ability of the monomeric peptide designated M118 to inhibit CD40L-induced expression of CD86 on primary B-cells in a concentration-dependent manner, with an IC50 of less than 0.5 μM. Similar results were obtained for a variant of the peptide having a C-terminal serine residue i.e., peptide M118S, for which the EC50 value was about 7.426×10-7 M (FIG. 5e). In contrast, a control peptide that does not function as a peptidyl inhibitor of the invention failed to provide significant concentration-dependent inhibition at micromolar concentrations (FIG. 6c). In these assays, CD40L induced CD86 expression in a concentration-dependent manner in the absence of a peptidyl inhibitor of the present invention with an EC50 value of about 9 nM (FIG. 6d, 6e). As a control in these assays, LPS-induced expression of CD86 mediated by the Toll-like receptors TLR 2/4, and TNFα-induced cytoxicity, were also assayed to exclude effects mediated by ligands other than CD40L. As shown in FIGS. 6f and 6g, no significant inhibition of LPS-induced CD86 expression was detected for peptide M118S and, as shown in FIGS. 6h-6j, none of the peptides designated M125, M14S, M16S, M17S, M19S or M118S provided significant inhibition of rTNFα-induced cytoxicity.









TABLE 6







Inhibition of CD40L-CD40 interaction by monomeric peptides


as determined by inhibition of CD40L-induced


CD86 expression on primary B-cells












EC50 unmodified
EC50 PEGylated



Peptide
peptide (nM)
peptide (nM)















M1_6S

1.5



M1_9S

180



M1-18S

90



M1_30S

>2000



M1_42S

5000



M1_50S

30



M1_82rd

470



M7_206rd

2,000



M7_217
2,500







S denotes C-terminal serine;



Cys denotes N-terminal cysteine






In a similar series of bioassays, dimeric forms of the CD40L peptide inhibitors were also tested for their ability to inhibit or reduce CD40L-induced expression of CD86 on primary B-cells. Representative data are provided in FIGS. 7a-7e and Table 7.









TABLE 7







Enhanced inhibition of CD40L-CD40 interaction


by dimeric peptides as determined by inhibition of


CD40L-induced CD86 expression on primary B-cells










Peptide
EC50 (M)







M1_6S
 3.23 × 10−7



Cys_M1_6S
3.065 × 10−7



M1_4S
1.616 × 10−6



M1_9S
5.643 × 10−7



M1-18S
7.426 × 10−7



M7_189S
1.765 × 10−7



Cys_M1_6S homodimer
4.891 × 10−8



Cys_M1_6S-Cys_M1_4S heterodimer
6.121 × 10−8



Cys_M1_6S-Cys_M1_9S heterodimer
3.505 × 10−8



Cys_M1_6S-Cys_M1_189S heterodimer
1.396 × 10−7







S denotes C-terminal serine;



Cys denotes N-terminal cysteine






2. T-Cell Proliferation Assays

An antigen-specific T-cell proliferation assay was used to measure the ability of anti-CD40L-peptides to inhibit CD40L receptor activity displayed natively on the surface of T cells.


Briefly, pooled DLN (consisting parathymic and posterior mediastinal nodes) were collected from transgenic D011.10 mice engineered to express ovalbumin-specific T cell receptors (TCRs). Enriched T-cell suspensions were prepared and stimulated with 10 μg/ml ovalbumin in the presence or absence of 10 μM or 20 μM concentration of anti-CD40L peptidyl inhibitors. The peptides tested were PEGylated serine derivatives and/or chiral analogs of peptides CD40L M16, CD40L M19, CD40L M118, CD40L M130, CD40L M142, CD40L M150, CD40L M782 and CD40L M7206. Cells were cultured for 24-72 hrs and then pulsed with [3H]thymidine for another 24 hr to measure T-cell proliferation.


Representative data indicate that, compared to positive control assays i.e., lacking inhibitory peptide, the peptides tested provided about 30% to about 75% inhibition of T-cell proliferation. Te best-performing peptidyl inhibitors in this assay were PEGylated chiral analogs of CD40L M130s and CD40L M142s. In general, PEGylated chiral analogs of serine derivatives performed better than PEGylated serine derivatives.


Example 2
Structural Features of Representative Peptidyl Inhibitors

This example provides additional representative peptidyl inhibitors of the present invention selected on the basis of whether or not they correspond to natural open reading frames, and used to interrogate the PDB structural database to identify or resolve secondary structures and assemblies of secondary structures in silico. The resolved secondary structures form a basis for homology modelling, peptide docking and rational drug design e.g., of small molecules.


Table 8 provides the amino acid sequences of 286 peptidyl inhibitors of the present invention identified using methods such as described in Example 1 hereof. Table 8 also indicates the bacterial origins of all sequences which are encoded by natural open reading frames present in the libraries from which they were derived. Table 9 indicates whether or not certain the amino acid sequences of Table 8 are encoded by bacterial genomes from which they are derived, and provides the Uniref Accession Nos. of the proteins encoded by those open reading frames.


The sequences presented in Tables 8 and 9, including those that were determined to be encoded by natural open reading frames, were aligned to identify regions of overlap and ascertain minimum sequences e.g., consensus domains, required for interaction between the peptides and CD40L. Data showing regions of overlap are presented in Table 10.


In particular, the data presented in Table 10 indicate two type of overlapping sequence, as follows:


In one example, Table 10 demonstrates overlapping clones identified independently to bind CD40L are encoded by different fragments of the same bacterial open reading frame i.e., from the same organism source, thereby providing an internal validation to the screening procedure. Exemplary clones in this category are inter alia clones encoding different fragments of glycyl-tRNA synthetase from B. pertussis; clones encoding different fragments of glycogen debranching enzyme from R. sphaeroides; clones encoding different fragments of ABC peptide transporter from R. sphaeroides; clones encoding different fragments of iron-sulfur protein from B. pertussis; and clones encoding different fragments of aliphatic amidase from Rhodopseudomonas palustris. Other such homologies are apparent from the data presented in Table 10.


In another example, Table 10 demonstrates overlapping clones identified independently to bind CD40L are encoded by different fragments of open reading frames from different bacteria that are predicted to encode the same functional protein e.g., glycyl-tRNA synthetase from B. pertussis, R. sphaeroides and D. vulgaris; ABC transporter from B. pertussis and P. auruginosa; and 3-hydroxydecanoyl-(acyl carrier protein) dehydratase from R. sphaeroides and C. crescentus. Other such homologies are apparent from the data presented in Table 10.


Secondary structures are derived from regions of overlapping primary sequence. By way of non-limiting example, data shown in FIG. 8a show that the region of overlap between the amino acid sequences of different clones that align to the glycyl tRNA synthetases of Thermotoga maritime, Desulfovibrio vulgaris, Rhodobacter sphaeroides and Bordetella pertussis are predicted to form an anti-parallel B sheet structure. In another example, data presented in FIG. 8b show that the region of overlap between the amino acid sequences of different clones that align to the glycogen debranching enzyme GlgX of Rhodobacter sphaeroides are predicted to form an anti-parallel B sheet structure. In yet another example, data presented in FIG. 9 show that the predicted secondary structure of the peptidyl inhibitor M07 40L 0103 0859 aligns to a secondary structural region of a Salmonella enterica protein. In yet another example, data presented in FIG. 10 and FIG. 11 indicate that regions of overlapping primary structures between peptidyl inhibitors of the invention and known proteins for which secondary structures have been resolved by crystal structure determination may be mapped accurately to the resolved secondary structural regions in the known protein, thereby permitting resolution of the predicted secondary structure for the peptidyl inhibitor. In FIG. 10, the secondary structure of the peptidyl inhibitor M08 40L 0103 0716 is derived by alignment to the crystal structure of a ferric alcaligin siderophore receptor. In FIG. 11, the secondary structure for the peptidyl inhibitor M09 40L 0103 0755 is derived by alignment with the crystal structure of a benzoate 1,2-dioxygenase beta subunit polypeptide. Accordingly, the available data indicate that particular peptidyl inhibitors of the present invention form conserved secondary structures or assemblies of secondary structures present in correctly-folded proteins.











TABLE 8






Bacterial



Clone ID
Origin
Peptide Sequence (SEQ ID NO:)







M08_40L_0103_0716

Bordetella

ELRTKQTGAYLVGRFALAEPLHLMVGDRWSDWKTKQMYFGSRREYRIKNQFTPYAGLTYDI




pertussis

NDTYTAYASYTEIFQPQNARDTSGGILPPIKSNSc (SEQ ID NO: 45)





M08_40L_0103_0717

Thermotoga

ELLHLPRPGFcDARSHGDSNFEDLFYFILcGTHFNSc (SEQ ID NO: 46)




maritima







M08_40L_0103_0718

Pseudomonas

ELLLNGANQYSPDAQPWAGVPLAIADSDGEFSEEVEDYLWEELELLDDLHELWRVFLTLST




aeruginosa

SSDHPLNAEYLDEVLKDKEVFRRDFNSc (SEQ ID NO: 47)





M08_40L_0103_0719

Deinococcus

ELRDGNFDDTDRVGTVHDMRFVFLDNDTKLLFcTAYDDEWDPYIDDFATKIPDELDLFKcN




radiodurans

Sc (SEQ ID NO: 48)





M08_40L_0103_0720

Geobacter

ELRLPETRKAQAALATKYGIYGFcYYHYWFNGRRILESPVDAMLESGEPDFPFMLcWANEN




sulfurreducens

WTSNSc (SEQ ID NO: 49)





M08_40L_0103_0721

Bacillus

ELLWRLNEWNYSDAELLSLIEWcIDHGNSc (SEQ ID NO: 50)




subtilis







M08_40L_0103_0722
Unidentified
ELHPEQKRLSTLSFYKEAQDEVTFYRDWDADSDSPDGELFGGSLANFEPTLETYDPDDAAP




WAWNLPSDLFQEQFENWSEFHKILQNSNSc (SEQ ID NO: 51)





M08_40L_0103_0723

Thermotoga

ELPHLPRPGFcDARSHGDSNFEDLFYFILcGTHFNSc (SEQ ID NO: 52)




maritima







M08_40L_0103_0724

Caulobacter

ELLMRDWNYPGWVMSDWAcENDFLLNKVLKRDWNYPGWVMSDcGNSc 




vibrioides

(SEQ ID NO: 53)



(crescentus)






M08_40L_0103_0725

Haloarcula

ELLWNDGVSKEPFYEMDADLHLIDPNALIDWLGAWDQSDLTEIRENVAPFLGNLIFRQTDD




marismortui

WHDYRYYSNSc (SEQ ID NO: 54)





M08_40L_0104_0726

Streptomyces

ELQAYGFTGGVcSPEELWELVASGGDAIGEFPAGRGWDLEGLFDSDPDRSGTSYARYGGFL




avermitilis

YEAGEFDADFFGISPREALAMDPQSNSc (SEQ ID NO: 55)





M08_40L_0104_0727
Unidentified
ELLSWVTDNcDFTGSITERPYNGPWMQFDRDIDPSVLDPQADSDVSLDEVVEYDcRIDETD




LEEWISDHEEFPDLVSLLDISVDGERWIPLHGIYKWRADEGNSc 




(SEQ ID NO: 56)





M08_40L_0104_0729
Unidentified
ELGVWMDGWVcGWLGLDEWIGTRVEGGQRDVDGcNSc (SEQ ID NO: 57)





M08_40L_0104_0730

Rhodobacter

ELLRPHDPEKAKALLAEAGVSDVSLDYVVNAGNEVDEQIAVLLQQQLGQAGITVNLQKMDP




sphaeroides

SMTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNMNYRRNSc 




(SEQ ID NO: 58)





M08_40L_0104_0732

Pseudomonas

ELLSSGSTVAKPPRMMKSPAIAGRcRDVHDDQERQIPGGFcHHLGLYcRWYGNcSWSIDDA




aeruginosa

LNLDPSGLDNDSWRSPYAFAGQYRFNRDWRINSDFNSc (SEQ ID NO: 59)





M08_40L_0104_0733

Geobacter

ELPLDEVNYSYPVVAIKEIHVWKSQDYDSGYPYPTPAPYYYYDPYWYGVWPGPYWHRPLGP




sulfurreducens

VRRNSc (SEQ ID NO: 60)





M08_40L_0104_0734

Pseudomonas

ELLSAPDMLLLDEPTNHLDADSVAWLEHFLHDFPGTVVAITHDRYFLDNVAGWILELDRGH




aeruginosa

GIPFEGNSc (SEQ ID NO: 61)





M08_40L_0104_0735
Unidentified
ELLMNEESTEFIARGHLTDDWDKVFFDSLNGGMEYGPGSFLGMGSPFNLMDHFSVHRYYQA




GGDTDFTDEQYYKIFARNSc (SEQ ID NO: 62)





M08_40L_0104_0736

Bacillus

ELRHDGYTFSPHQAMPKDEFGEWPVPVFPNGDcYFFFHQDFSWGLLGDPWKcAITVFGESN




subtilis

Sc (SEQ ID NO: 63)





M08_40L_0104_0737

Haloarcula

ELLPYEELDGVSIDLDGLTQLWNDGVSKEPFYEMDADLHLIDPNALIDWLGAWDQSDLTGN




marismortui

Sc (SEQ ID NO: 64)





M08_40L_0104_0738

Streptomyces

ELHFKPKQLLGLTATPEWMDGLNVQDKFFEGRIAAELRLWEALENDLLcPFHYFGIPDGTD




avermitilis

LTSNSc (SEQ ID NO: 65)





M08_40L_0104_0739

Streptomyces

ELLLQGEDMIEQLGRAHMSWGLGSADRWDLDQTTGIITWTFPDKTAAAPAQILGSFSPGSG




avermitilis

SWLWAWANKSNSc (SEQ ID NO: 66)





M08_40L_0104_0740
Unidentified
ELRVEWDYWYPVDYRFSGNDLITNHLTFYQFHHGELFDEPQWPRGIVIMGNSc 




(SEQ ID NO: 67)





M08_40L_0104_0741
Unidentified
LELLFGGSLVNLEPTLETYDPDDAAPWAWNLPSDLFQEQFENWSEFHKILQNSNSc 




(SEQ ID NO: 68)





M08_40L_0104_0742

Halobacterium

ELPcYDMHGLPIETKVEEQLGFESKKDIQEFGEEAFIEEcKRFADDNLDGLQSDFQSFGVW




salinarum

MDWDNPYKTVDPSYMEAAWWAFSEVQSATALAGDGSSNSc (SEQ ID NO: 69)





M08_40L_0104_0743

Caulobacter

ELLARSGNWKNLWDDAIGcVRPRYPNGEWVENYScTYDYPDRSGPWWDAVFYEGNSLQYSS




vibrioides

FVPQDVAGLMANTGGPDGFVKWLDHLFDGHYSQSNSc (SEQ ID NO: 70)



(crescentus)






M08_40L_0104_0744

Bordetella

ELLLDEPTNHLDAESVEWLEQFLHKFPGTVVAVTHDRYFLDNAAEWILELDRGYGIPWKGN




pertussis

Sc (SEQ ID NO: 71)





M08_40L_0104_0745

Streptomyces

ELPTYAFERERFWLDVEEGSAGGSGVSGMWGGPLWEAVEcGDAGAcELWNWSLDVLLSNSc 




avermitilis

(SEQ ID NO: 72)





M08_40L_0104_0746
Unidentified
ELLcQPLALSGIDNPFEWIDIAKGAPHVSISLGEGVDIYSPDDEPASNGPLPDDVLDLFWc




FGNSc (SEQ ID NO: 73)





M08_40L_0104_0747

Pseudomonas

ELLAEHAVWSLKcFPDWEWYNINIFGTDDPNHFWVEcDGHGKILFPGYPEGYYENHFLHSF




aeruginosa

ELEDGNSc (SEQ ID NO: 74)





M08_40L_0104_0748

Salmonella

ELRIESLEGEMWLINPFNGETLDEHTLEVWLKGNSc (SEQ ID NO: 75)




enterica





(typhinurium)






M08_40L_0104_0749

Desulfovibrio

ELLIDRWYWPNDVPSSLVPGYNATPNPDVGGPLFGDNEDLNQIVDEGWWIFYEESRNSC 




vulgaris

(SEQ ID NO: 76)





M08_40L_0104_0750

Pseudomonas

ELLWEIGVDQEPDLGYSFPKPTVARLHNGKWAVVTGNGYSSLNDKAALLIIDLETGAITRN




aeruginosa

Sc (SEQ ID NO: 77)





M09_40L_0103_0752

Deinococcus

ELLcRVSAAEPPAGGRAAVRLLOGYLWYPEGADVOLESFLPRELDLSQAPSLSEEDAHVLW




radiodurans

DQVQPPFAFFENGN (SEQ ID NO: 78)





M09_40L_0103_0753

Deinococcus

ELLRDGNFDDTDRVGTVHDMRFVFLDNDTKLLFcTAYDDEWDPYIDDFATKIPDELDLFIS




radiodurans

N (SEQ ID NO: 79)





M09_40L-0103_0754
Unidentified
ELRMISYcMASDPFGHAVRDYYLGELEEPLIDRDGDETREHSIEEWYFGEYQRDEWFESWL




EGPLLDMGN (SEQ ID NO: 80)





M09_40L_0103_0755

Pseudomonas

ELLcREARYLDDKDWDAWLALYAADASFWMPSWDDRDQLTEDPQREISLIWYGN 




aeruginosa

(SEQ ID NO: 81)





M09_40L_0103_0756

Pseudomonas

ELPWVIEHLEAEGVLLPDLEQAEEDVAVQLVFGAEVVVQVGARQFGFEGDVAHGGAGVAFF




aeruginosa

GEDFFGGQENLLDVAAADLDLVcAHVRSITANSTKATSN (SEQ ID NO: 82)





M09_40L_0103_0757

Bordetella

ELLWWVEDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMD




pertussis

EAWKWIDDPAFAEFAGDQQLTIRN (SEQ ID NO: 83)





M09_40L_0103_0758

Bordetella

ELLFRAPEFDFSDYVLDHVEVHRcNYNWKTFIEVYLEDYHVGN (SEQ ID NO: 84)




pertussis







M09_40L_0103_0759

Haloarcula

ELLFDHFRFcLTEFDRFDFSDHHGYLERNDWTIHDFAGNGATGQFAVELTPDIIEETYRKA




marismortui

QDSANAVGDTPASREFEFKRYYYSRN (SEQ ID NO: 85)





M09_40L_0103_0760
Unidentified
ELRWLVGTYSYQNDAYRQLFEPDDESALLQELSEYLDDHGSEPIIYYGGNYFDEQcLSRRF




DEHGN (SEQ ID NO: 86)





M09_40L_0103_0761
Unidentified
ELLFGGSLVNLEPTLETYDPDDAAPWAWNLPSDLFQEQFENWSEFHKILQNSN 




(SEQ ID NO: 87)





M09_40L_0103_0762
Unidentified
ELQRLAERYLSESYWGDVIEASDDVWELVAcPVDGALDAALWDAWLESLEEGRYSN 




(SEQ ID NO: 88)





M09_40L_0103_0763

Bordetella

ELLDLFGDFNGLPEGADRTEFYQHEGHWQNRMILGDSLQVMASLAEREGLRGKVQcIYFDP




pertussis

PYGIKFNSN (SEQ ID NO: 89)





M09_40L_0103_0764

Caulobacter

ELLWDDAIGcVRPRYPNGEWVENYScTYDYPDRSGPWWDAVFYEGDSLQYSSFVPQDVAGL




vibrioides

MANTGGPDGFVKWLDHLFDGHYSQSN (SEQ ID NO: 90)



(crescentus)






M09_40L_0103_0765

Salmonella

ELLGVWMGEPATLcTMQSTcGQSLLVEQNGDVFScDHFVFPAYKLGNLQQLHVDLAGSDLG




enterica

WRWRHYEPILLLRGWPRSSHSRKWDSTDSN (SEQ ID NO: 91)



(typhinurium)






M09_40L_0103_0766

Bordetella

ELPGFEHAIEDQLLDTLGHHFGDLFARPVDAWFHDLPHWYDHDIRSN 




pertussis

(SEQ ID NO: 92)





M09_40L_0104_0767

Haloarcula

ELLGEYEHAARYVSEVEcNWKTFAGNYSEcDHcHANHQDWITDIELAEPELEVNDYHWILH




marismortui

YTHDEDVEDEMRIHDEHEAKFYYFWPNFTGN (SEQ ID NO: 93)





M09_40L_0104_0768

Bordetella

ELLFQESLAGHGLSVPRFFLRPPVHAVcPLHRWTYDGQGRILGAPHFPSTPcLNLSRFPLH




pertussis

NcHGLLFEGPRDPLKDLOVLFRRPEFDFSTLQNPFLADLVSLADPRSEYSYLNYcN 




(SEQ ID NO: 94)





M09_40L_0104_0769

Desulfovibrio

ELLFHGHDQFSQVEGVcAEVFNERGFGLDFLGGHAELVHDDLLDFFFNGHGSLQcN 




vulgaris

(SEQ ID NO: 95)





M09_40L_0104_0770

Streptomyces

ELHPVGETGPQLNATDHFHSTGHPVIRSFEPGEGWFWDYTTSELYESGPALAPPPNATDHF




avermitilis

HSTGHPVIRSFEPGEGWFWDYTSN (SEQ ID NO: 96)





M09_40L_0104_0771

Rhodopseudomonas

ELLQNYALIHDQDFGGWQQWWDLHNVEGEPSTGLLVEDGNLALQAALDGLGIALLRPSLVD




palustris

VFVDEGGLSRLFDHQLEDGRDYYLcHLVEQPLSEAEERN (SEQ ID NO: 97)





M09_40L_0104_0772
Invalid genome
ELPLAAGTSWWEKELFMGAPDWSQFEKYPYPSLSPEEQSFIDNEVEVLcSN 




(SEQ ID NO: 98)





M09_40L_0104_0773
Unidentified
ELPKFDEYLSDDNNPDEPGWFMQWLQQTcDLFAYTFDEAWFDIGTPQSN 




(SEQ ID NO: 99)





M09_40L_0104_0774

Haloarcula

ELPLGEYEHAARYVSEVEcNWKTFAGNYSEcDHcHANHQDWITDIELAESELEVNDYHWIL




marismortui

HYTHDEDVEDEMRIHDEHEAKFYYFWPNFTSN (SEQ ID NO: 100)





M09_40L_0104_0775

Bordetella

ELLDLFGDFNGLPEGADRTEFYQHEGHWQNRMILGDSLQVMASLAEREGLRGKVQcIYFDP




pertussis

PYGIKFNSN (SEQ ID NO: 101)





M09_40L_0104_0776

Halobacterium

ELLQTSAFEPDYVGERLDEWAWIVYPWNFIEDLEELLEGEDDPEHRQKEYIAWTKSSDWKc




salinarum

N (SEQ ID NO: 102)





M09_40L_0104_0777
Invalid genome
ELLWcQHGGFKYGTSLTDMFDQFKSEYcDGcN (SEQ ID NO: 103)





M09_40L_0104_0778

Rhodopseudomonas

ELRGcHSNTHQYAFSPGSPFFPFAISLPWRHDSDVEAAPcN (SEQ ID NO: 104)




palustris







M09_40L_0104_0780

Salmonella

ELLWPESWGGLPPASFFDELDPcINRHLRYPLFSETFTADLPVGTLcN 




enterica

(SEQ ID NO: 105)



(typhinurium)






M09_40L_0104_0781
Unidentified
ELLLERLNGVSVDWSNLYNSWNPDKETLYELDSDLHLADPAYVSTMDAWDTADVEEVQTEI




APWFGNSLSRNHSEPPADWADQYQYYSLWDIYGN (SEQ ID NO: 106)





M09_40L_0104_0782

Bacteroides

LELLAPELVELcDEMGFMMMIEPFDEWDIAKcENGYHRYFNEWAERDMVNMLHNYRNNPcV




thetaiotaomicron

VMWSIGNEVPTQGN (SEQ ID NO: 107)





M09_40L_0104_0783

Bordetella

ELLLLDEPTNHLDAESVEWLEQFLHKFPGTVVAVTHDRYFLDNAAEWILELDRGYGIPcN 




pertussis

(SEQ ID NO: 108)





M09_40L_0104_0784
Unidentified
ELLWIGINTNGMQWNGMQWQRMEWNGMEWHKPEWYGMEWNGMEWNGMEWKGIEcN 




(SEQ ID NO: 109)





M09_40L_0104_0785

Streptomyces

ELLWDVKTYVSDQDGTGWDLVEQYQNKYGMPNPDGTIGKTLWLDYIQcN 




avermitilis

(SEQ ID NO: 110)





M09_40L_0104_0786

Clostridium

ELLPEcAYTYGIDNILSEFGIKYFISEGKAIDYASPKSMYGTNTPIAAPSGVcAFGRDMDS




acetobutylicum

SYQVWSDFMGYPGN (SEQ ID NO: 111)





M09_40L_0104_0787

Porphyromonas

ELPAVGLGYEFFQGDFYScYAQGGVGYGMEYNSVSYPQEYDVSVKRFGWLAELGGDYFRRN 




gingivalis

(SEQ ID NO: 112)





M09_40L_0104_0788

Streptomyces

ELHcScYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDcYYVVEKHSN 




avermitilis

(SEQ ID NO: 113)





M09_40L_0104_0789

Haloarcula

ELLLAEQAGTLKSELEAMPLGEYEHAARYVSEVEcNWKTFAGNYSEcDHcHANHQDWITDI




marismortui

ELEESELEVNDYHWILHYTHDEDVEDEMRIHDEHEAKFYYFWPNFTGN 




(SEQ ID NO: 114)





M09_40L_0104_0790

Streptomyces

ELLcSSNNRTYYFEEHcScYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDcYYVVEK




avermitilis

QcN (SEQ ID NO: 115)





M09_40L_104_0791

Rhodobacter

ELLAYGKSTEDKQDFLLFHVNLDPHAAQTFEFEVPLWEFGLPDDASVEVEDLLNGNRFTWH




sphaeroides

GKWQWLELDPQTRPYAVWRLYAPGMPRCN (SEQ ID NO: 116)





M06_40L_0103_0801
Unidentified
LWTSSSDLDDAAPWVWHLPNDLSQDPFEDWAELHRIPQKQSVR 




(SEQ ID NO: 117)





M06_40L_0103_0807

Rhodobacter

LMMDRITDISADGGLHGKGHVVAEFDIHPDLWFFEcHFP (SEQ ID NO: 118)




sphaeroides







M06_40L_0104_0818

Halobacterium

LTRTDRADWESVNEAccWWcEREYFWGARNSc (SEQ ID NO: 119)




salinarum







M06_40L_0104_0820

Salmonella

QLETLTEWMDWSLADRDVDLDGIYYcPHHPQSNSc (SEQ ID NO: 120)




enterica





(typhinurium)






M06_40L_0104_0825

Streptomyces

HKEYNYWEKHKDDKYYYWNTYKEYNYWEKHKDDKcYWNEKDTKSNSc 




avermitilis

(SEQ ID NO: 121)





M06_40L_0104_0829
Unidentified
LSDYEcPPVFLSGGDVPRcWVAVRGFEFFGRDFRcGEV (SEQ ID NO: 122)





M06_40L_0104_0840
Unidentified
PGQFQLTRVFSDYEcPPVFLSGGDVPRcWVAVRGFEFFGRDFRcGEV 




(SEQ ID NO: 123)





M06_40L_0104_0842

Rhodopseudomonas

RYENKEWVWGYRESEPMGGYDPYSSSKGcAELVTTAYSNSc 




palustris

(SEQ ID NO: 124)





M07_40L_0104_0853

Caulobacter

RFFKRDLNNFcYQADTFNAYcN (SEQ ID NO: 125)




vibrioides





(crescentus)






M07_40L_0104_0859

Bacteroides

QFEEGLERTVRWYLDNEVWMDNVTSGDYQEYYDSIY (SEQ ID NO: 126)




thetaiotaomicron







M07_40L_0104_0880

Rhodobacter

LNDLDNVGYTARHHTFFEMLGNFSF (SEQ ID NO: 127)




sphaeroides







M07_40L_0104_0895

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHIPK (SEQ ID NO: 128)




sphaeroides







M08_40L_0105_0897
Unidentified
ELLLQNGTSSMVIFDPLAPGMLEDPYSTYAILRSGDPVHWHDGLKAWVLTGHRDcLYVLQN




PDSFScNcWAGEPSSEAHTDTYFETNENWIMVNSFNTGNYGGcPMNQMAAIDDFTALYNDH




PSNSc (SEQ ID NO: 129)





M08_40L_0105_0898

Streptomyces

ELHcScYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDcYYVVEKHSNSc 




avermitilis

(SEQ ID NO: 130)





M08_40L_0105_0899

Aeropyrum

ELLIPLKWSIRYYIcYRGLAAYSGcFGGEALSEEALPFEERYYPDAERYLGYYSNSc 




pernix

(SEQ ID NO: 131)





M08_40L_0105_0900

Streptomyces

ELRTPGSSHNYcWDDHYNSYYVVQNHKYYWDYHYDcYYVVEKHcNSc 




avermitilis

(SEQ ID NO: 132)





M08_40L_0105_0901

Geobacter

ELPKRSMIVAMSTVITLDFILFHTTSSLLGSFDSNSc (SEQ ID NO: 133)




sulfurreducens







M08_40L_0105_0907

Bacillus

ELFAFFSSRFFSVDDccSHFLSSYDcSISDLMLERTPFTNFVALSSPNRFASNSc 




subtilis

(SEQ ID NO: 134)





M08_40L_0105_0913

Streptomyces

ELRYYFEEHcScYYYTENDHNcYWDDHYNSYYVVQYNHKYYWDYHYDcYYVVEKHSNSc 




avermitilis

(SEQ ID NO: 135)





M08_40L_0105_0914

Pseudomonas

ELPNPKEWDELPGLAVFHGLDNSFDNEFQcSIRVMSFMSGFLVcNSc 




aeruginosa

(SEQ ID NO: 136)





M08_40L_0105_0915

Chlorobium

ELLLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIRPGNSc 




tepidum

(SEQ ID NO: 137)





M09_40L_0105_0918

Streptomyces

ELLFNHKYYWDYHYDcYYVVEKHcKYYYWNTYKEYNYWEKHKDDKcYWNYHYDcYYVVEKH




avermitilis

cKYYYWNTYKEYNYWEKHKDSN (SEQ ID NO: 138)





M09_40L_0105_0919

Rhodobacter

ELRWEVWcDGMEVSQFTYFQQVGGHDcRPVSGELTYGLERLAMYVLGIDHVMDMPFNDPcG




sphaeroides

PTPLTYGN (SEQ ID NO: 139)





M09_40L_0105_0920

Desulfovibrio

ELLKHSDLFcELPDKFYDSAFLDRIHFYIPGWEVDIIRGEMFSNSN 




vulgaris

(SEQ ID NO: 140)





M09_40L_0105_0925

Neisseria

ELLYADVAVSGFAFDMVEAGALFAQDFYGLVHFGITDGSGYFFNFLcRQIADNDFGEHFKN




menigitidis

GGN (SEQ ID NO: 141)





M09_40L_0105_0926

Bordetella

LVSKPDMLLLDEPTNHLDAESVEWLEQFLHKFPGTVVAVTHDRYFLDNAAEWILELDRGYG




pertussis

IPWK (SEQ ID NO: 142)





M09_40L_0105_0927

Streptomyces

ELRcYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDcYYVVEKHcN 




avermitilis

(SEQ ID NO: 143)





M09_40L_0105_0932

Pseudomonas

ELRcFFEFLWRDLPGPGSSAESSPQPGN (SEQ ID NO: 144)




aeruginosa







M09_40L_0105_0933
Unidentified
ELLSLYcRNHRVEcFccHTGGDRSELPSTHYSTSSGFMQVYDFFGVPFVLEYSN 




(SEQ ID NO: 145)





C03_40L_0105_0953

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 146)




sphaeroides







C03_40L_0105_0955

Rhodobacter

PLFSENATRVELcLFDETGQTQTHcLDLPSYEGGIWYGYLPGIREGQTYGYRVHGPHAPEE


(M09)

sphaeroides

GHRFNPN (SEQ ID NO: 147)





C03_40L_0205_0991

Rhodobacter

LGADFDGEGTNFPLFSENATRVELcLFDETGQTQTHcLDLPSYEGGIWYGYLLGADFDGEG




sphaeroides

SNSc (SEQ ID NO: 148)





C02_40L_0204_0993

Rhodopseu-

LGYPDLDLIVFPEYSTQGLNTAIWTYDEMLLTVDSPEIGVFYYFGEGTV 




domonas

(SEQ ID NO: 149)




palustris







M09_40L_0204_1008

Chlorobium

LFIGAPNLHWPDTINSWLEEDRVLFTcDSFGcHYcNEAMYDDLc 




tepidum

(SEQ ID NO: 150)





M09_40L_0205_1012

Caulobacter

LMFDRIVRIEAEGGKYGKGYVEAEFDIRPDLWFFDcHFIGDPVMPGcLGLDAMWQLVGFFQ 




crescentus

(SEQ ID NO: 151)





M09_40L_0205_1019

Caulobacter

LIHEAIGDQLTcVFVDTGLLRKNEADQVVTLFRDHYNIPLVHVDAGDLFLGELAGVSDPET




crescentus

K (SEQ ID NO: 152)





C02_40L_0304_1021

Haloarcula

PEDRFFWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH 




marismortui

(SEQ ID NO: 153)





C02_40L_0304_1023

Rhodopseu-

PELAREAAYKGANVYIRISGYSTOVNDQWIWTNRTNAWQNLMYTMSVNLAGYDGVFYYFGE




domonas

GTVcNYDGNVIQQ (SEQ ID NO: 154)




palustris







C02_40L_0403_1038

Salmonella

LWHESWGGLPPASFFDELDPcINRHLRYPLFSETFTADLRGEAcSNSc 




enterica

(SEQ ID NO: 155)





C02_40L_0405_1049

Bordetella

LLEDDWENPTLGAWGLGWEVWLNGMEVTKFTYFQQVGGLDcTPTTGEITYGLERLAMYLQD




pertussis

VESVYDLVWTEGAN (SEQ ID NO: 156)





C02_40L_0504_1064

Bordetella

LVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDcTPTTGEITYGLERLAMYLQD




pertussis

VESVYDLVWTEGAN (SEQ ID NO: 157)





C02_40L_0504_1066

Bordetella

LFRRPEFDFSDYVLDHVEVHRcNYNWKTFIEVYLEDYHVGPFHPGLGRFVTc 




pertussis

(SEQ ID NO: 158)





C02_40L_0504_1067

Bordetella

LKALGIDPTQHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCTPTTGE




pertussis

ITY (SEQ ID NO: 159)





C02_40L_0505_1070

Bordetella

RFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLD (SEQ ID NO: 160)




pertussis







C02_40L_0505_1073

Desulfovibrio

LYLGSLRALGIDPAAHDIRFVEDDWESPTLGAWGPGWEVWLN (SEQ ID NO: 161)




vulgaris







C02_40L_0604_1085

Rhodobacter

LVNGEYDLSVMYWTNDILDPDQKTIFVLGHDVNMTWDMLVNGEYDLSVMYWTNDILDPDQK




sphaeroides

TTFVLGHDVNMSNSc (SEQ ID NO: 162)





C02_40L_0604_1089

Haloarcula

QTAKEIHFGFDQKPEDRFFWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEM




marismortui

IERH (SEQ ID NO: 163)





C02_40L_0605_1092
Unidentified
PGVDLGVQLLFQTVEcGDGVSGVSWFDELSYVDFAIDAEKFQGPGQFQLTRVFGDYEcPPV




FLSGGDVPRcWVAVRGFEFFS (SEQ ID NO: 164)





C02_40L_0705_1112
Unidentified
LWDWIPFPGTEGIYFYRDWDADSDSPDGELFGGSLVNLELTLETYDPDDAAPWAWNLPSDL




FQEQYENWSEFHKILQN (SEQ ID NO: 165)





C02_40L_0804_1146

Streptomyces

RLMHcLWEIIDNSVDEALGGYcDHIDVILHDDGSVEVRDNGR (SEQ ID NO: 166)




avermitilis







C02_40L_0804_1170

Rhodobacter

PLFSENATRVELcLFDETGQTQTHcLDLPSYEGGIWYGYLPGIREGQTYGYRAHGPHAPEE


(M08)

sphaeroides

GHRFNPN (SEQ ID NO: 167)





M09_40L_0203_1004

Haloarcula

LcEYEHAARYVSEVEcNWKTFAGNYSEcDHcHANHQDWITDIELAESELEVNDYHWILHcT




marismortui

HDEDVEDEMRIHDEHEAKFYYFWPNFN (SEQ ID NO: 168)





C02_40L_0803_1145
Unidentifed
RGIEVGDPSSGNETGPTGKPFTTTIPSEVGATEISGSGKEIQPAQLMNDLPNSESAEQVRE




RTRDLVQWFNYALPDFVFVEEDSGYWGDTQDFSMPTGDDYELNTcIFSWYWcGLWScDNRG




GRIVcLRENRYTSEYTGWcN (SEQ ID NO: 169)





C02_40L_0803_0889
Unidentifed
LPKYGTDEKQDALRRYYAAYFNVEGGDSGTFTDYKWDcLWScDNRGGRIVcLRENRYTSEY




TGWcN (SEQ ID NO: 170)





M09_40L_0203_1000

Pseudomonas

LLDHFRFCLTEFDRFDFSDHHGYLERNDWTIHDFVGNGATGQFAVELTPDIIEETY 




aeruginosa

(SEQ ID NO: 171)





C02_40L_0803_1116

Caulobacter

LIHEAIGDQLTcVFVDTGLLRKNEADQVVTLFRDHYNIPLVHVDAGDLFLGELAGVSDPET




crescentus

N (SEQ ID NO: 172)





C02_40L_0504_1056

Caulobacter

LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK 




vibrioides

(SEQ ID NO: 173)



(crescentus)






C02_40L_0605_1091

Caulobacter

LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK 




vibrioides

(SEQ ID NO: 174)



(crescentus)






C02_40L_0704_1098

Caulobacter

LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK 




vibrioides

(SEQ ID NO: 175)



(crescentus)






C02_40L_0803_1124

Caulobacter

LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK 




vibrioides

(SEQ ID NO: 176)



(crescentus)






C02_40L_0803_1136

Caulobacter

LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK 




vibrioides

(SEQ ID NO: 177)



(crescentus)






C02_40L_0803_1138

Caulobacter

LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDRK 




vibrioides

(SEQ ID NO: 178)



(crescentus)






C02_40L_0803_1144

Caulobacter

LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK 




vibrioides

(SEQ ID NO: 179)



(crescentus)






C02_40L_0804_1160

Caulobacter

LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK 




vibrioides

(SEQ ID NO: 180)



(crescentus)






C02_40L_0804_1174

Caulobacter

LWTLQVTGPDGVETYTTNFLWTcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK 




vibrioides

(SEQ ID NO: 181)



(crescentus)






C02_40L_0804_1161

Streptomyces

RLMHcLWEIIDNSVDEALGGYcDHIDVILHDDGSVEVRDK (SEQ ID NO: 182)




avermitilis







C02_40L_0205_0996

Chlorobium

LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIRLGNFYFIYR




tepidum

DGYWFcN (SEQ ID NO: 183)





C02_40L_0304_1027

Chlorobium

LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIRL 




tepidum

(SEQ ID NO: 184)





C02_40L_0803_1137

Haloarcula

FWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH 




marismortui

(SEQ ID NO: 185)





C02_40L_0505_1075

Haloarcula

FWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH 




marismortui

(SEQ ID NO: 186)





M07_40L_0104_0882

Rhodobacter

LLGVIVDGKEQTIIDDGNNEFGRKVSGDLDGTARFRWYLGNQTAADDYLLESYGEHPQFPW




sphaeroides

TTQHILK (SEQ ID NO: 187)





C03_40L_0104_0943

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 188)




sphaeroides







C03_40L_0104_0944

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 189)




sphaeroides







C03_40L_0104_0945

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 190)




sphaeroides







C03_40L_0104_0948

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHIRK (SEQ ID NO: 191)




sphaeroides







C03_40L_0104_0951

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 192)




sphaeroides







C03_40L_0104_0953

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 193)




sphaeroides







C03_40L_0105_0957

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHIHK (SEQ ID NO: 194)




sphaeroides







C03_40L_0105_0958

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 195)




sphaeroides







C03_40L_0105_0959

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 196)




sphaeroides







C03_40L_0105_0960

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 197)




sphaeroides







C03_40L_0105_0962

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILKG (SEQ ID NO: 198)




sphaeroides







C03_40L_0105_0964

Rhodobacter

RWYLGNQTAADDYLLESYDEHPQFPWTTQHILK (SEQ ID NO: 199)




sphaeroides







C03_40L_0105_0965

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 200)




sphaeroides







C03_40L_0105_0966

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 201)




sphaeroides







C03_40L_0105_0967

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHIHK (SEQ ID NO: 202)




sphaeroides







C03_40L_0105_0969

Rhodobacter

ELLLGVIVDGKEQTIIDDGNNEFGRKVSGDLDGTARFRWYLGNQTAADDYLLESYGEHPQF




sphaeroides

PWTTQHILKGN (SEQ ID NO: 203)





C03_40L_0105_0970

Rhodobacter

RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK (SEQ ID NO: 204)




sphaeroides







C02_40L_0404_1047

Bacteroides

PHTEQDLFRFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTYcN 




thetaiotaomicron

(SEQ ID NO: 205)





M09_40L_0205_1014

Bacteroides

LIQSDIGNIcFTPHTEQDLFcFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTF 




thetaiotaomicron

(SEQ ID NO: 206)





C02_40L_0104_0977

Bacteroides

PHTEQDLFRFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTY 




thetaiotaomicron

(SEQ ID NO: 207)





C02_40L_0804_1154

Bacteroides

LIQSDIGNICFTPHTEQDLFRFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTF 




thetaiotaomicron

(SEQ ID NO: 208)





C02_40L_0804_1147

Pseudomonas

LIRWDRcVVGEGcDHLScSGLINNAHTNSITNLISNPFSGITLAcINPTSScFGIPFGARG




aeruginosa

RRN (SEQ ID NO: 209)





C02_40L_0804_1156

Pseudomonas

LIRWDRcVVGEGcDHLScSGLINNAHTNSITNLISNPFSGITLAcINPTSScLGN 




aeruginosa

(SEQ ID NO: 210)





C02_40L_0705_1111

Pseudomonas

LIRWDRcVVGEGcDHLScSGLINNAHTNSITNLISNPFSGITLAcINPTSScL 




aeruginosa

(SEQ ID NO: 211)





C02_40L_0804_1163

Pseudomonas

LIRWDRcVVGEGcDHLScSGLINNAHTDSITNLISNPF 




aeruginosa

(SEQ ID NO: 212)





C02_40L_0304_1030

Bordetella

LWWVFDNPNDcLDFSRPGKYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTIRKKNGLGVFSTQMPSSLL (SEQ ID NO: 213)





C02_40L_0604_1084

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTIRKKNGLGVFSTQMP (SEQ ID NO: 214)





C02_40L_0704_1103

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTI (SEQ ID NO: 215)





C02_40L_0704_1106

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTT (SEQ ID NO: 216)





C02_40L_0704_1104

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISTYLLHRMSEAMDGRAFVYLMDEA




pertussis

WKWIDDPAFAEFA (SEQ ID NO: 217)





M09_40L_0103_0757

Bordetella

WWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAW




pertussis

KWIDDPAFAEFAGDQQLTI (SEQ ID NO: 218)





C02_40L_0604_1086

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTI (SEQ ID NO: 219)





C02_40L_0604_1081

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTI (SEQ ID NO: 220)





C02_40L_0504_1061

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTI (SEQ ID NO: 221)





C02_40L_0404_1043

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTI (SEQ ID NO: 222)





C02_40L_0304_1022

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTI (SEQ ID NO: 223)





M09_40L_0105_0928

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTI (SEQ ID NO: 224)





M09_40L_0105_0923

Bordetella

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEA




pertussis

WKWIDDPAFAEFAGDQQLTI (SEQ ID NO: 225)





C02_40L_0103_0972

Caulobacter

LYDYPDRSGPWWDAVFYEGNSLQYPSFVPQDVAGLMANTGGPDGFVKWLDHLFDGHYSQSN




crescentus

EPDLLAPYLYIQRNSc (SEQ ID NO: 226)





C02_40L_0204_0990

Streptomyces

FKPKQLLGLTATPERMDGLNVQDEFFEGRIAAELRLWEALENDLLCPFHYFGIPDGTDLT 




avermitilis

(SEQ ID NO: 227)





M06_40L_0104_0819

Streptomyces

FKPKQLLGLTATPERMDGLNVQDEFFEGRIAAELRLWEALENDLLCPFHYFGIPDGTDLT 




avermitilis

(SEQ ID NO: 228)





M09_40L_0203_1004

Haloarcula

LcEYEHAARYVSEVEcNWKTFAGNYSEcDHcHANHQDWITDIELAESELEVNDYHWILHcT




marismortui

HDEDVEDEMRIHDEHEAKFYYFWPNFN (SEQ ID NO: 229)





C02_40L_0803_1126

Rhodobacter

LAYGKSTEDKQDFLLFHVNLDPHAAQTFEFEVPLWEFGLPDDASVEVEDLLNGNRFTWHGK




sphaeroides

WQWLELDPQT (SEQ ID NO: 230)





C02_40L_0204_0994

Rhodobacter

LAYGKSTEDKQDFLLFHVNLDPHAAQTLEFEVPLWGFGLPDDASVEVEDLLNGDRFTWHGK




sphaeroides

WQWLELDPQT (SEQ ID NO: 231)





C02_40L_0104_0974

Pseudomonas

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ 




aeruginosa

(SEQ ID NO: 232)





C02_40L_0105_0982

Pseudomonas

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ 




aeruginosa

(SEQ ID NO: 233)





C02_40L_0105_0984

Pseudomonas

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ 




aeruginosa

(SEQ ID NO: 234)





C02_40L_0305_1033

Pseudomonas

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ 




aeruginosa

(SEQ ID NO: 235)





C02_40L_0305_1034

Pseudomonas

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ 




aeruginosa

(SEQ ID NO: 236)





C02_40L_0305_1035

Pseudomonas

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ 




aeruginosa

(SEQ ID NO: 237)





C02_40L_0404_1041

Pseudomonas

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ 




aeruginosa

(SEQ ID NO: 238)





C02_40L_0705_1113

Pseudomonas

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ 




aeruginosa

(SEQ ID NO: 239)





C02_40L_0305_1032

Porphyromonas

RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSW




gingivalis

GHYLNAYDAFPGHNR (SEQ ID NO: 240)





C02_40L_0504_1057

Porphyromonas

QVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSWGHYLN




gingivalis

AYDAFPDYN (SEQ ID NO: 241)





C02_40L_0505_1074

Porphyromonas

RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSW




gingivalis

GHYLNAYDAFPGHNR (SEQ ID NO: 242)





C02_40L_0505_1076

Porphyromonas

RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSW




gingivalis

GHYLNAYDAFPGHNR (SEQ ID NO: 243)





C02_40L_0605_1094

Porphyromonas

RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSW




gingivalis

GHYLNAYDAFPGHNR (SEQ ID NO: 244)





C02_40L_0705_1110

Porphyromonas

RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSW




gingivalis

GHYLNAYDAFPGHNR (SEQ ID NO: 245)





C02_40L_0804_1171

Porphyromonas

RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSW




gingivalis

GHYLNAYDAFPGHNR (SEQ ID NO: 246)





M06_40L_0103_0810

Rhodobacter

LKEIADNANVQKVAFDRYKIKYFKRDMIDcGFDERWIDEHMVSYGQGFEKV 




sphaeroides

(SEQ ID NO: 247)





M06_40L_0104_0837

Rhodobacter

LKEIADNANVQKVAFDRYKIKYFKRDMIDcGFDERWIDEHMVSYGQGFVSMG 




sphaeroides

(SEQ ID NO: 248)





M06_40L_0104_0822

Streptomyces

PFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT 




avermitilis

(SEQ ID NO: 249)





M06_40L_0104_0830

Streptomyces

PFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT 




avermitilis

(SEQ ID NO: 250)





M07_40L_0103_0860

Streptomyces

PFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT 




avermitilis

(SEQ ID NO: 251)





M07_40L_0104_0864

Streptomyces

LSGGPDHVPHPEHSTYDFPFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT 




avermitilis

(SEQ ID NO: 252)





M07_40L_0104_0866

Streptomyces

PQPEHSTYDFPFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT 




avermitilis

(SEQ ID NO: 253)





M07_40L_0104_0879

Streptomyces

LGGGPDHVPHPEHSTYDFPFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT 




avermitilis

(SEQ ID NO: 254)





M07_40L_0104_0884

Streptomyces

PFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT 




avermitilis

(SEQ ID NO: 255)





M07_40L_0104_0896

Streptomyces

LGGGPDHVPHPEHSTYDFPFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRM 




avermitilis

(SEQ ID NO: 256)





M08_40L_0105_0903

Streptomyces

YKEYNYWE--------------------KHKDDKCYW 




avermitilis

(SEQ ID NO: 257)





M08_40L_0105_0904

Streptomyces

YKEYNYWEKHKDD--------------------KCYWNEKDTKN 




avermitilis

(SEQ ID NO: 258)





M09_40L_0105_0922

Streptomyces

YKEYNYWE--------------------KHKDDKCYWNDGSTR 




avermitilis

(SEQ ID NO: 259)





M09_40L_0105_0924

Streptomyces

YKEYNYWEKHKDD--------------------KCYWNEKDTKN 




avermitilis

(SEQ ID NO: 260)





M09_40L_0105_0930

Streptomyces

RCYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKHCKYYYWNTYKEYNYWE




avermitilis

KHKDDKYIEGTFKVVTNAAI (SEQ ID NO: 261)





M09_40L_0105_0934

Streptomyces

CYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKHCKYYYWNTYKEYNYWEK




avermitilis

HKDDK (SEQ ID NO: 262)





M07_40L_0103_0847

Streptomyces

HYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKHC 




avermitilis

(SEQ ID NO: 263)





M06_40L_0103_0805
Unidentified
RWDRPWYSPVTNWSPHCRGLD (SEQ ID NO: 264)





M06_40L_0104_0835
Unidentified
WDRPWYSPVTNWSPHCRGLD (SEQ ID NO: 265)





M06_40L_0103_0811

Pseudomonas

RGTSWGTACPWWFPTTTALTR (SEQ ID NO: 266)




aeruginosa







M06_40L_0104_0826

Pseudomonas

RGTSWGTACPWWFPTTTALTR (SEQ ID NO: 267)




aeruginosa







M06_40L_0103_0812

Geobacter

LSCSWCSFFPDTKSVSCQD (SEQ ID NO: 268)




sulfurreducens







M06_40L_0104_0838

Geobacter

LSCSWCSFFPDTKSVSCQD (SEQ ID NO: 269)




sulfurreducens







M07_40L_0104_0874

Streptomyces

LWDVKTYVSDQDGTGWDLVEQYQNKYGMPNPDGTIGKTLWLDYIQ 




avermitilis

(SEQ ID NO: 270)





M07_40L_0104_0883

Rhodopseudomonas

RCEDQVDVIAGHRPGDFLLRATLAMDPRVKPADDGGGWSWWFL 




palustris

(SEQ ID NO: 271)





M07_40L_0104_0885

Rhodopseudomonas

REVLQLDDHVHVSPALAVQELLAKPHVGRRAVSVDVIAGHRPGDFLLRATLAMDPRVKPAD




palustris

DGGGWSWWFI (SEQ ID NO: 272)





M07_40L_0104_0889
Unidentified
LPKYGTDEKQDALRRYYAAYENVEGGDSGTFTDYKWDCLWSCDNRGGRIVCLRENRYTSE




(SEQ ID NO: 273)





C02_40L_0803_1123
Unidentified
LWWLcYQDDEISTEATNEIGLPKYGTDEKQDALRKYYAAYFNVEGGDSGTFTDYKWDcFWA




HRHAIMH (SEQ ID NO: 274)





C02_40L_0803_1120
Unidentified
LWWLcYQDDEISTEATNEIGLPKYGTDEKQDALRKYYAAYFNVEGGDSGTFTDYKWDcFWA




HRHAIMH (SEQ ID NO: 275)





C02_40L_0803_1145
Unidentified
RGIEVGDPSSGNETGPTGKPFTTTIPSEVGATEISGSGKEIQPAQLMNDLPNSESAEQVRE




RTRDLVQWFNYALPDFVFVEEDSGYWGDTQDFSMPTGDDYELNTcIFSWYWcGLWScDNRG




GRIVcLRENRYTSEYTGW (SEQ ID NO: 276)





C03_40L_0103_0942

Thermotoga

HLPRPGFCDARSHGDSNFEDLFYFILCGTH (SEQ ID NO: 277)




maritima







C02_40L_0504_1062

Geobacter

RIPETRKAQAALATKYGIYGFCYYHYWFNGRRILESPVDAMLESGEPDFPFMLCWANENWT




sulfurreducens

(SEQ ID NO: 278)





M08_40L_0105_0902

Streptomyces

LLGEDMIEQLGRAHMSWGLGSADRWDLDQTTGIITWTFPDETAAAPAQILGSFSPGSGSWL




avermitilis

WAWANK (SEQ ID NO: 279)





M08_40L_0105_0909

Pseudomonas

LAEHAVWSLKCFPDWEWYNINIFGTDDPNHFWVECDGHGKILFPGYPEGYYENHFLHSFEL




aeruginosa

ED (SEQ ID NO: 280)





C02_40L_0205_0998

Streptomyces

LGHIWASDVENAASFEPVDVGDEEAYKAGLLWLERLTMSDNGLRQLALFEWDEDGNLTKEW




avermitilis

HAE (SEQ ID NO: 281)





M08_40L_0105_0910

Streptomyces

LGHIWASDVENAASFEPVDVGDEEAYKAGLLWLERLTMSDNGLRQLALFEWDEDGNLTKEW




avermitilis

HAE (SEQ ID NO: 282)





M08_40L_0105_0912
Unidentified
LVCTYSYQNDAYRQFFEPDDESALLQELSEYLDDHGSEPIIHYGGNYFDEQCLSRRFDE




(SEQ ID NO: 283)





C02_40L_0304_1027

Chlorobium

LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIR




tepidum

(SEQ ID NO: 284)





C02_40L_0205_0996

Chlorobium

LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIRLGNFYFIYR




tepidum

DGYWF (SEQ ID NO: 285)





M09_40L_0204_1006
Probable
LGDINPLKRIVDSRQSKRLAERYLSESYWGDVIEASDDVWELVAcPVDGALDAALWDAWLE



Halo. Sp.
SLEEG (SEQ ID NO: 286)





C02_40L_0603_1077
Unidentified
LWIGINTNGMQWNGMQWQRMEWNGMEWHKPEWYGMEWNGMEWNGMEWKGIE




(SEQ ID NO: 287)





M09_40L_0105_0935
Unidentified
LWIGINTNGMQWNGMQWQRMEWNGMEWHKPEWYGMEWNGMEWNGMEWKGIE




(SEQ ID NO: 288)





M09_40L_0105_0931
Unidentified
LWIGINTNGMQWNGMQWQRMEWNGMEWHKPEWYGMEWNGMEWNGMEWKGIE




(SEQ ID NO: 289)





C03_40L_0203_1175
Unidentified
PRMEWNVLQWNGMESNELVSNGTEWNGMDWNAMEWNRMEWNGMEWNQSEWNGRELNGMEWK




GMEWNGMEWNGTNPSGME (SEQ ID NO: 290)





M09_40L_0204_1010
Invalid
RNEVEWNGMERNGMEWSGMELNGTQWNEVEWSRMEWNGWEWNGMEWNGMEWNGEEWSGVE



genome.
(SEQ ID NO: 291)





M07_40L_0104_0893
Unidentified
LWNGIIRNGMERNGMEWNGMEWNGMEWNGMEWVRIEWNGMDSNGIAWNGMDSNAMERNALE




WNGMDSKAMEWNGIDWNGMEWNGLEWNHHRMESNGIIEWNRMESSNRLERNRQ 




(SEQ ID NO: 292)





M09_40L_0204_1005
Unidentified
PWNEMEWKGIEWNQPEWNGMERNGMEWNGMEWNGMEWNQLDWNGMEWNGLE




(SEQ ID NO: 293)





C02_40L_0104_0979
Unidentified
PWNEMEWKGIEWNQPEWNGMERNGMEWNGMEWNGMEWNQLDWNGMEWNGLE




(SEQ ID NO: 294)





C02_40L_0804_1169
Unidentified
RLEWNGMELNGITPSEMAWKGTEYNLMEWNGINPSGMEWIGMEWNGMEWKGMEWNGMEWFQ




LE (SEQ ID NO: 295)





M06_40L_0104_0839
Unidentified
PGVVRScVEWSGIDWScEELcGVEWNGVEWKGVEWNGMEWNGMELNGREWSGTEENGVEWS




GVERSGSW (SEQ ID NO: 296)





C02_40L_0705_1114
Unidentified
LWcEWELEWNGMEWNGMEWNGMEWNAMEcNGFNSIAMEWNAMEWNQPE




(SEQ ID NO: 297)





M09_40L_0205_1016
Unidentified
LSLYCRNHRVECFCCHTGGDRSELPSTHYSTSSGFMQVYDFFGVPFVLEY




(SEQ ID NO: 298)





C02_40L_0104_0977

Bacteroides

PHTEQDLFRFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTY




thetaiotaomicron

(SEQ ID NO: 299)





M09_40L_0205_1014

Bacteroides

LIQSDIGNIcFTPHTEQDLFcFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTF




thetaiotaomicron

(SEQ ID NO: 300)





C02_40L_0404_1047

Bacteroides

PHTEQDLFRFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTY 




thetaiotaomicron

(SEQ ID NO: 301)





C02_40L_0804_1154

Bacteroides

LIQSDIGNIcFTPHTEQDLERFDSRSLPIFLYDDSVREHFYYFcIQVESDSTF




thetaiotaomicron

(SEQ ID NO: 302)





C02_40L_0105_0983
Probable
QVLRTLQVVTcRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN



Halo. Sp.
(SEQ ID NO: 303)





C02_40L_0105_0985
Probable
QVLRTLQVVTcRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN



Halo. Sp.
(SEQ ID NO: 304)





C02_40L_0105_0986
Probable
QVLRTLQVVTcRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN



Halo. Sp.
(SEQ ID NO: 305)





C02_40L_0105_0987
Probable
QVLRTLQVVTcRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN



Halo. Sp.
(SEQ ID NO: 306)





C02_40L_0203_0988

Halorubrum

LWWDGTVAGLEYFTAGFDGFEIEWADAAGEYGFTKERLYELDSDLHLVDPVWVTMQDNWNR




lacusprofundi

SDTDEVADNIGPWFGNYY (SEQ ID NO: 307)





C02_40L_0204_0992

Haloarcula

LLGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWcPVcGREVFSHIPFEGVFcK 




marismortui

(SEQ ID NO: 308)





C02_40L_0505_1072

Haloarcula

LGRDGWPVSIGPDSQMSLEVIDRESEALFEFLWcPVcGHEVFSHIPFEGVFc 




marismortui

(SEQ ID NO: 309)





C02_40L_0303_1020
Unidentified
LFGSRPLSRScLELLHEESEISVEAVVTHPEGHDGWWDGALRSKAEEYGYPVIEENEVFEc




ELDYIISVLYYEILDAELLEHPKQGGLNLHQAELPRYRG (SEQ ID NO: 310)





C02_40L_0503_1053
Unidentified
LFGSRPLSRScLELLHEESEISVEAVVTHPEGHDGWWDGALRPKAEEYGYPVIEENEVFEc




ELDYIISVLYYEILDAELLEHPKQGGLNLHQAELPRYRG (SEQ ID NO: 311)





C02_40L_0704_1100
Unidentified
LFGSRPLSRScLELLHEESEISVEAVVTHPEGHDGWWDGALRSKAEEYGYPVIEENEVFEc




ELDYIISVLYYEILDAELLEHPKQGGLNLHQAELPRYRG (SEQ ID NO: 312)





C02_40L_0304_1028
Unidentified
LcDPNGRAEAIPEAKSDVTVTHQFITIWSEWIRMDVLMTGVYGRcGTAVIDHLHDDDAYDF




TYLN (SEQ ID NO: 313)





C02_40L_0404_1045
Unidentified
LGDPNGRAEAIPEAKSDVTVTHQFITIWSEWIRMDVLMTGVYGRcGTAVIDHLHDDDAYDF




TYFN (SEQ ID NO: 314)





C02_40L_0405_1048

Caulobacter

LYEYNADTPTSIYEAGVFQWLWLEDMIQQGALPEATDQFNSLHDQLAERFKAIFPNGGFVH




crescentus

FA (SEQ ID NO: 315)





C02_40L_0604_1080

Caulobacter

LYEYNADTPTSIYEAGVFQWLWLEDMIQQGALPEATDQFNSLHDQLAERFKAIFPN




crescentus

(SEQ ID NO: 316)





C02_40L_0803_1122

Haloarcula

PVEKLNNLTGTPLVASFTLFVcQSGQNTLLEcTLIV (SEQ ID NO: 317)




marismortui







C02_40L_0804_1172

Haloarcula

PVEKLNNLTGTPLVASFTLFVcQSGQNTLLEcTLIV (SEQ ID NO: 318)




marismortui







C02_40L_0803_1125

Streptomyces

LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGA




avermitilis

LWVIL (SEQ ID NO: 319)





C02_40L_0803_1127

Streptomyces

LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGA




avermitilis

LWVIL (SEQ ID NO: 320)





C02_40L_0804_1148

Streptomyces

LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGA




avermitilis

LWVIL (SEQ ID NO: 321)





C02_40L_0804_1149

Streptomyces

LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGA




avermitilis

LWVIL (SEQ ID NO: 322)





C02_40L_0804_1152

Streptomyces

LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGA




avermitilis

LWVIL (SEQ ID NO: 323)





C02_40L_0804_1155

Streptomyces

LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGA




avermitilis

LWVIL (SEQ ID NO: 324)





C02_40L_0804_1162

Streptomyces

LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGA




avermitilis

LWVIL (SEQ ID NO: 325)





C02_40L_0804_1164

Streptomyces

LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGA




avermitilis

LWVIL (SEQ ID NO: 326)





C02_40L_0804_1151

Pyrococcus

LVQGSKEATTIDESAELEALADAVAEEILSSDGIYFLGAGSTIKRIKDKIGIEGTLLGVDV




horikoshii

VRVENGKAKLLVKDA (SEQ ID NO: 327)





C02_40L_0804_1173

Pyrococcus

LVQGSKEATTIDESAELEALADAVAEEILSSDGIYFLGAGSTIKRIKDKIGIEGTLLGVDV




horikoshii

VRVENGKAKLLVKDA (SEQ ID NO: 328)





C02_40L_0804_1157

Salmonella

LVcGWTDEDEIGLFVQVGAILRGESEITWGEPLYLSGVVT (SEQ ID NO: 329)




enterica







C02_40L_0804_1165

Salmonella

LVcGWTDEDEIGLFVQVGAILRGESEITWGEPLYLSGVVT (SEQ ID NO: 330)




enterica

















TABLE 9







Uniref Accession Nos. for clones shown in Table 8









Clone ID (SEQ ID NO:)
Natural ORF
Uniref





M08_40L_0103_0716 (SEQ ID NO: 45)
Yes
Q9X6A5


M08_40L_0103_0717 (SEQ ID NO: 46)
No


M08_40L_0103_0718 (SEQ ID NO: 47)
Yes
C1VB01


M08_40L_0103_0719 (SEQ ID NO: 48)
Yes
A5WES7


M08_40L_0103_0720 (SEQ ID NO: 49)
Yes
Q74D11


M08_40L_0103_0721 (SEQ ID NO: 50)
Yes
P42972


M08_40L_0103_0722 (SEQ ID NO: 51)
No
C1VIM5


M08_40L_0103_0723 (SEQ ID NO: 52)
No


M08_40L_0103_0724 (SEQ ID NO: 53)
Yes
B8GWA5


M08_40L_0103_0725 (SEQ ID NO: 54)
Yes
Q5V6U1


M08_40L_0104_0726 (SEQ ID NO: 55)
Yes
Q9S0R4


M08_40L_0104_0727 (SEQ ID NO: 56)
No
C1VB01


M08_40L_0104_0729 (SEQ ID NO: 57)
No


M08_40L_0104_0730 (SEQ ID NO: 58)
Yes
B9KTA7


M08_40L_0104_0732 (SEQ ID NO: 59)
Yes
Q02TG8


M08_40L_0104_0733 (SEQ ID NO: 60)
Yes
Q74C58


M08_40L_0104_0734 (SEQ ID NO: 61)
Yes
B7V0D9


M08_40L_0104_0735 (SEQ ID NO: 62)
No
C1VSP2


M08_40L_0104_0736 (SEQ ID NO: 63)
Yes
P42301


M08_40L_0104_0737 (SEQ ID NO: 64)
Yes
Q5V6U1


M08_40L_0104_0738 (SEQ ID NO: 65)
Yes
Q82CY6


M08_40L_0104_0739 (SEQ ID NO: 66)
Yes
Q82J42


M08_40L_0104_0740 (SEQ ID NO: 67)
Yes
C1V6Z2


M08_40L_0104_0741 (SEQ ID NO: 68)
No
C1VIM5


M08_40L_0104_0742 (SEQ ID NO: 69)
Yes
Q9HN97


M08_40L_0104_0743 (SEQ ID NO: 70)
Yes
B8GZZ8


M08_40L_0104_0744 (SEQ ID NO: 71)
Yes
Q2KVV9


M08_40L_0104_0745 (SEQ ID NO: 72)
Yes
Q9S0R3


M08_40L_0104_0746 (SEQ ID NO: 73)
No


M08_40L_0104_0747 (SEQ ID NO: 74)
Yes
A3L7V2


M08_40L_0104_0748 (SEQ ID NO: 75)
Yes
A8AG05


M08_40L_0104_0749 (SEQ ID NO: 76)
Yes
Q72CL7


M08_40L_0104_0750 (SEQ ID NO: 77)
Yes
A8WE64


M09_40L_0103_0752 (SEQ ID NO: 78)
Yes
Q9RSG8


M09_40L_0103_0753 (SEQ ID NO: 79)
Yes
B5HSV1


M09_40L_0103_0754 (SEQ ID NO: 80)
No
C1VM70


M09_40L_0103_0755 (SEQ ID NO: 81)
Yes
A3KUS5


M09_40L_0103_0756 (SEQ ID NO: 82)
No


M09_40L_0103_0757 (SEQ ID NO: 83)
Yes
Q7VSX9


M09_40L_0103_0758 (SEQ ID NO: 84)
Yes
Q7WCN7


M09_40L_0103_0759 (SEQ ID NO: 85)
Yes
Q5UWS9


M09_40L_0103_0760 (SEQ ID NO: 86)
No


M09_40L_0103_0761 (SEQ ID NO: 87)
No
C1VIM5


M09_40L_0103_0762 (SEQ ID NO: 88)
No
C1VWC8


M09_40L_0103_0763 (SEQ ID NO: 89)
Yes
Q7VUH9


M09_40L_0103_0764 (SEQ ID NO: 90)
Yes
B8GZZ8


M09_40L_0103_0765 (SEQ ID NO: 91)
Yes
B4T3X7


M09_40L_0103_0766 (SEQ ID NO: 92)
No


M09_40L_0104_0767 (SEQ ID NO: 93)
Yes
Q5V5Z3


M09_40L_0104_0768 (SEQ ID NO: 94)
Yes
Q7WCN7


M09_40L_0104_0769 (SEQ ID NO: 95)
No


M09_40L_0104_0770 (SEQ ID NO: 96)
No
Q9K4L8


M09_40L_0104_0771 (SEQ ID NO: 97)
Yes
B3QIR9


M09_40L_0104_0772 (SEQ ID NO: 98)
Yes
B0VUU9


M09_40L_0104_0773 (SEQ ID NO: 99)
No
C1VBM9


M09_40L_0104_0774 (SEQ ID NO: 100)
Yes
Q5V5Z3


M09_40L_0104_0775 (SEQ ID NO: 101)
Yes
Q7VUH9


M09_40L_0104_0776 (SEQ ID NO: 102)
Yes
B0R354


M09_40L_0104_0777 (SEQ ID NO: 103)
No


M09_40L_0104_0778 (SEQ ID NO: 104)
No


M09_40L_0104_0780 (SEQ ID NO: 105)
Yes
A9MYN9


M09_40L_0104_0781 (SEQ ID NO: 106)
No
Q5V6U3


M09_40L_0104_0782 (SEQ ID NO: 107)
Yes
A7V0W9


M09_40L_0104_0783 (SEQ ID NO: 108)
Yes
Q7W4S4


M09_40L_0104_0784 (SEQ ID NO: 109)
No
B6KWB1


M09_40L_0104_0785 (SEQ ID NO: 110)
Yes
Q82RE3


M09_40L_0104_0786 (SEQ ID NO: 111)
Yes
Q97GF3


M09_40L_0104_0787 (SEQ ID NO: 112)
Yes
Q7MXP8


M09_40L_0104_0788 (SEQ ID NO: 113)
Yes
Q82EY2


M09_40L_0104_0789 (SEQ ID NO: 114)
Yes
Q5V5Z3


M09_40L_0104_0790 (SEQ ID NO: 115)
Yes
Q82EY2


M09_40L_0104_0791 (SEQ ID NO: 116)
Yes
A3PIQ3


M06_40L_0103_0801 (SEQ ID NO: 117)
Yes
C1VIM5


M06_40L_0103_0807 (SEQ ID NO: 118)
Yes
Q3IXE1


M06_40L_0104_0818 (SEQ ID NO: 119)
No


M06_40L_0104_0820 (SEQ ID NO: 120)
Yes
Q8ZRM8


M06_40L_0104_0825 (SEQ ID NO: 121)
Yes
Q82EY2


M06_40L_0104_0829 (SEQ ID NO: 122)
No


M06_40L_0104_0840 (SEQ ID NO: 123)
No


M06_40L_0104_0842 (SEQ ID NO: 124)
Yes
Q6N2K1


M07_40L_0104_0853 (SEQ ID NO: 125)
Yes
Q9ABL7


M07_40L_0104_0859 (SEQ ID NO: 126)
Yes
Q8AAJ8


M07_40L_0104_0880 (SEQ ID NO: 127)
Yes
Q3J0R1


M07_40L_0104_0895 (SEQ ID NO: 128)
Yes
Q3J233


M08_40L_0105_0897 (SEQ ID NO: 129)
Yes
B4T0P0


M08_40L_0105_0898 (SEQ ID NO: 130)
Yes
Q82EY2


M08_40L_0105_0899 (SEQ ID NO: 131)
Yes
Q9Y8M8


M08_40L_0105_0900 (SEQ ID NO: 132)
No
Q82EY2


M08_40L_0105_0901 (SEQ ID NO: 133)
Yes
Q74C20


M08_40L_0105_0907 (SEQ ID NO: 134)
No


M08_40L_0105_0913 (SEQ ID NO: 135)
Yes
Q82EY2


M08_40L_0105_0914 (SEQ ID NO: 136)
No


M08_40L_0105_0915 (SEQ ID NO: 137)
Yes
Q8KCV5


M09_40L_0105_0918 (SEQ ID NO: 138)
Yes
Q82EY2


M09_40L_0105_0919 (SEQ ID NO: 139)
Yes
Q3J5C9


M09_40L_0105_0920 (SEQ ID NO: 140)
Yes
Q72AH6


M09_40L_0105_0925 (SEQ ID NO: 141)
No


M09_40L_0105_0926 (SEQ ID NO: 142)
Yes
Q7W4S4


M09_40L_0105_0927 (SEQ ID NO: 143)
Yes
Q82EY2


M09_40L_0105_0932 (SEQ ID NO: 144)
No


M09_40L_0105_0933 (SEQ ID NO: 145)
No


C03_40L_0105_0953 (SEQ ID NO: 146)
Yes
Q3J233


C03_40L_0105_0955 (M09) (SEQ ID NO:
Yes
Q3J4A2


147)


C03_40L_0205_0991 (SEQ ID NO: 148)
Yes
Q3J4A2


C02_40L_0204_0993 (SEQ ID NO: 149)
Yes
Q6N8I0


M09_40L_0204_1008 (SEQ ID NO: 150)
Yes
Q8KA83


M09_40L_0205_1012 (SEQ ID NO: 151)
Yes
Q9A246


M09_40L_0205_1019 (SEQ ID NO: 152)
Yes
Q9A7U9


C02_40L_0304_1021 (SEQ ID NO: 153)
Yes
Q5V498


C02_40L_0304_1023 (SEQ ID NO: 154)
Yes
Q6N8I0


C02_40L_0403_1038 (SEQ ID NO: 155)
Yes
P06188


C02_40L_0405_1049 (SEQ ID NO: 156)
Yes
Q7W0Q6


C02_40L_0504_1064 (SEQ ID NO: 157)
Yes
Q7W0Q7


C02_40L_0504_1066 (SEQ ID NO: 158)
Yes
Q7VW15


C02_40L_0504_1067 (SEQ ID NO: 159)
Yes
Q7W0Q8


C02_40L_0505_1070 (SEQ ID NO: 160)
Yes
Q7W0Q9


C02_40L_0505_1073 (SEQ ID NO: 161)
Yes
A1VCW8




(Q72AU2)


C02_40L_0604_1085 (SEQ ID NO: 162)
Yes
Q3IX77


C02_40L_0604_1089 (SEQ ID NO: 163)
Yes
Q5V498


C02_40L_0605_1092 (SEQ ID NO: 164)
Yes


C02_40L_0705_1112 (SEQ ID NO: 165)
Yes
C1VIM5


C02_40L_0804_1146 (SEQ ID NO: 166)

Q82KG1


C02_40L_0804_1170 (M08) (SEQ ID NO:
Yes
Q3J4A2


167)
















TABLE 10





Alignments of peptides shown in Table 8 and 9







Glycyl-tRNA synthetase alignments








C02_40L_0405_1049
                   LLEDDWENPTLGAWGLGWEVWLNGMEVTKFTYFQQVGGLDcTPTTGEITYGLERLAMYLQDVESVYDLVWTEGAN


C02_40L_0504_1067
                   LVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDcTPTTGEITYGLERLAMYLQDVESVYDLVWTEGAN


C02_40L_0504_1064
     LKALGIDPTQHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCTPTTGEITY


C02_40L_0505_1070
                  RFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLD


M09_40L_0105_0919
                                   RWEVWcDGMEVSQFTYFQQVGGHDcRPVSGELTYGLERLAMYVLGIDHVMDMPFNDPcGPTPLTY


C02_40L_0505_1073
LYLGSLRALGIDPAAHDIRFVEDDWESPTLGAWGPGWEVWLN


Found Structure
LYLESLEYLGINLKEHDIRFVEDNWEEPTLGAWGVGWEVWLDGMEITQFTYFQQIGGISLKDIPLEITYGLERIAMYLQGVDNVYEVQWNENVKYGDVFLENEREF


Consensus sequence
                    VEDNWESPTLGAWGVGWEVWL(D/N)GME(I/V)(T/S)QFTYFQQ(I/V)GGX(D/S) (SEQ ID NO: 331)










Glycogen debranching enzyme alignments








C02_40L_0804_1170 (M08)
            PLFSENATRVELcLFDETGQTQTHcLDLPSYEGGIWYGYLPGIREGQTYGYRAHGPHAPEEGHRFNPN


C03_40L_0105_0955 (M09)
            PLFSENATRVELcLFDETGQTQTHcLDLPSYEGGIWYGYLPGIREGQTYGYRVHGPHAPEEGHRFNPN


C03_40L_0205_0991
LGADFDGEGTNFPLFSENATRVELcLFDETGQTQTHcLDLPSYEGGIWYGYLLGADFDGEGSNSc


Consensus sequence
            PLFSENATRVELcLFDETGQTQTHcLDLPSYEGGIWYGYL (SEQ ID NO: 332)










ABC peptide transporter alignments








C02_40L_0604_1085
LVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNMTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNMSNSc


C02_40L_0604_1085

LVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNMTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNMSNSc



M08_40L_0104_0730
LRPHDPEKAKALLAEAGVSDVSLDYVVNAGNEVDEQIAVLLQQQLGQAGITVNLQKMDPSMTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNMNYRRNSc


Consensus sequence
(SEQ ID NO: 333) MTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNM










Putative iron-sulfur protein and putative dioxygenase alpha subunit YeaW alignments








C02_40L_0504_1066
                                                                   LFRRPEFDFSDYVLDHVEVHRcNYNWKTFIEVYLEDYHVGPFHPGLGRFVTc


M09_40L_0103_0758
                                                                   LFRRPEFDFSDYVLDHVEVHRcNYNWKTFIEVYLEDYHV


M09_40L_0104_0768
SVPRFFLRPPVHAVcPLHRWTYDGQGRILGAPHFPSTPcLNLSRFPLHNcHGLLFEGPRDPLKDLDVLFRRPEFDFSTLQNPFLADLVSLADPRSEYSYLNY


M09_40L_0203_1004 
                                                                           LcEYEHAARYVSEVEcNWKTFAGNYSEcDHcHANHQDWITDI


Consensus sequence
                                                                   LFRRPEFDFS (SEQ ID NO: 334)


Consensus sequence
                                                                                           NWKTF (SEQ ID NO: 335)










Aliphatic amidase alignments








C02_40L_0204_0993
               LGYPDLDLIVFPEYSTQGLNTAIWTYDEMLLTVDSPEIGVFYYFGEGTV


C02_40L_0304_1023
PELAREAAYKGANVYIRISGYSTQVNDQWIWTNRTNAWQNLMYTMSVNLAGYDGVFYYFGEGTVcNYDGNVIQQ


Consensus sequence
                                                     GVFYYFGEGTV (SEQ ID NO: 336)










Unidentified alignment








C02_40L_0605_1092
PGVDLGVQLLFQTVEcGDGVSGVSWFDELSYVDFAIDAEKFQGPGQFQLTRVFGDYEcPPVFLSGGDVPRcWVAVRGFEFFS


M06_40L_0104_0840
                                           PGQFQLTRVFSDYEcPPVFLSGGDVPRcWVAVRGFEFFGRDFRcGEV


N06_40L_0104_0829
                                                    LSDYEcPPVFLSGGDVPRcWVAVRGFEFFGRDFRcGEV


Consensus sequence
                                                      DYEcPPVFLSGGDVPRcWVAVRGFEFF (SEQ ID NO: 371)










Putative uncharacterised protein alignment








C02_40L_0705_1112
       LWDWIPFPGTEGIYFYRDWDADSDSPDGELFGGSLVNLELTLETYDPDDAAPPAWNLPSDLFQEQYENWSEFHKILQN


M08_40L_0103_0722
HPEQKRLSTLSFYKEAQDEVTFYRDWDADSDSPDGELFGGSLANFEPTLETYDPDDAAPWAWNLPSDLFQEQFENWSEFHKILQN


M09_40L_0103_0761
                                    LFGGSLVNLEPTLETYDPDDAAPWAWNLPSDLFQEQFENWSEFHKILQN


M08_40L_0104_0741
                                    LFGGSLVNLEPTLETYDPDDAAPWAWNLPSDLFQEQFENWSEFHKILQN


M06_40L_0103_0801
                                              LWTSSSDLDDAAPWVWHLPNDLSQDPFEDWAELHRIPQKQSVR


Consensus sequence











Ribulokinase alignments








C02_40L_0403_1038

LWHESWGGLPPASFFDELDPcINEHLRYPLFSETFTADLRGEAcSNSc



M09_40L_0104_0780

LWPESWGGLPPASFFDELDPcINEHLRYPLFSETFTADLPVGTLcN



Consensus sequence

LWHESWGGLPPASFFDELDPCINRHLRYPLFSETFTADL (SEQ ID NO: 337)











Unidentified alignment








C02_40L_0803_1145
. . . RERTRDLVQWFNYALPDFVFVEEDSGYWGDTQDFSMPTGDDYELNTcIFSWYWcGLWScDNRGGRIVcLRENRYTSEYTGWcN


C02_40L_0803_0889
                                    LPKYGTDEKQDALRRYYAAYFNVEGGDSGTFTDYKWDcLWScDNRGGRIVcLRENRYTSEYTGWcN


Consensus sequence
                                                                          LWScDNRGGRIVcLRENRYTSEYTGWcN (SEQ ID NO: 372)










Extracellular solute binding protein and outer membrane protein alignments








M08_40L_0104_0730
LRPHDPEKAKALLAEAGVSDVSLDYVVNAGNEVDEQIAVLLQQQLGQAGITVNLQKMDPSMTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNMNYR


C02_40L_0604_1085
                           LVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNMTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNM


Consensus sequence
                                           (SEQ ID NO: 338) MTWDMLVNGEYDLSVMYWTNDILDPDQKTTFVLGHDVNM










Putative ABC transporter alignments








M09_40L_0105_0926
LVSKPDMLLLDEPTNHLDAESVEWLEQFLHKFPGTVVAVTHDRYFLDNAAEWILELDRGYGIPWK


M08_40L_0104_0744
        LLDEPTNHLDAESVEWLEQFLHKFPGTVVAVTHDRYFLDNAAEWILELDRGYGIPWK


M09_40L_0104_0783
       LLLDEPTNHLDAESVEWLEQFLHKFPGTVVAVTHDRYFLDNAAEWILELDRGYGIP


M08_40L_0104_0734
 LSAPDMLLLDEPTNHLDADSVAWLEHFLHDFPGTVVAITHDRYFLDNVAGWILELDRGHGIPFE


Consensus sequence
    PDMLLLDEPTNHLDA(E/D)SV(E/A)WLE(Q/H)FLH(K/D)FPGTVVA(V/I)THDRYFLDN(A/V)A(E/G)WILELDRG(Y/H)GIP (SEQ ID NO: 339)










Hypothetical protein rrnB0067 alignments








M09_40L_0103_0759
LFDHFRFCLTEFDRFDFSDHHGYLERNDWTIHDFAGNGATGQFAVELTPDIIEETYRKAQDSANAVGDTPASREFEFKRYYYS


M09_40L_0203_1000
LLDHFRFCLTEFDRFDFSDHHGYLERNDWTIHDFVGNGATGQFAVELTPDIIEETY


Consensus sequence
L(F/L)DHFRFCLTEFDRFDFSDHHGYLERNDWTIHDF(A/V)GNGATGQFAVELTPDIIEETY (SEQ ID NO: 340)










3-Hydroxydecanoly1-(acyl carrier protein) dehydratase alignments








M06_40L_0103_0807
LMMDRITDISADGGLHGKGHVVAEFDIHPDLWFFEcHFP


M09_40L_0205_1012
LMFDRIVRIEAEGGKYGKGYVEAEFDIRPDLWFFDcHFIGDPVMPGcLGLDAMWQLVGFFQ


Consensus sequence
LM(M/F)DRI(T/V)(D/R)ISA(D)GG(L/K)(H/Y)GKG(H/Y)V(V/E)AEFDIHPDLWFF(E/D)CHF (SEQ ID NO: 341)










Bifunctional GMP synthase/glutamine amidotransferase protein alignments








M09_40L_0205_1019
LIHEAIGDQLTcVFVDTGLLRKNEADQVVTLFRDHYNIPLVHVDAGDLFLGELAGVSDPETK


C02_40L_0803_1116
LIHEAIGDQLTcVFVDTGLLRKNEADQVVTLFRDHYNIPLVHVDAGDLFLGELAGVSDPETN


Consensus sequence
LIHEAIGDQLTcVFVDTGLLRKNEADQVVTLFRDHYNIPLVHVDAGDLFLGELAGVSDPET (SEQ ID NO: 342)










acyl-coenzyme A synthetase alignments








C02_40L_0304_1021
             PEDRFFWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH


C02_40L_0604_1089
QTAKEIHFGFDQKPEDRFFWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH


Consensus sequence
             PEDRFFWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH (SEQ ID NO: 343)










monooxygenase flavin-binding family protein alignments








C02_40L_0504_1056
LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK


C02_40L_0605_1091
LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK


C02_40L_0704_1098
LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK


C02_40L_0803_1124
LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK


C02_40L_0803_1136
LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK


C02_40L_0803_1138
LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDRK


C02_40L_0803_1144
LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK


C02_40L_0804_1160
LWTLQVTGPDGVETYTTNFLWMcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK


C02_40L_0804_1174
LWTLQVTGPDGVETYTTNFLWTcQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLDLK


Consensus sequence
LWTLQVTGPDGVETYTINFLW(M/T)CQGYYRHSVGYTPEWPGMADFGGSIVHPQTWPADLD(L/R)K (SEQ ID NO: 344)










DNA topoisomerase IV subunit B alignments








C02_40L_0804_1146
RLMHcLWEIIDNSVDEALGGYcDHIDVILHDDGSVEVRDNGR


C02_40L_0804_1161
RLMHcLWEIIDNSVDEALGGYcDHIDVILHDDGSVEVRDK


Consensus sequence
RLMHcLWEIIDNSVDEALGGYcDHIDVILHDDGSVEVRD (SEQ ID NO: 345)










Putative uncharacterized protein alignment








C02_40L_0205_0996

LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIRLGNFYFIYRDGYWFcN



C02_40L_0304_1027

LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIRL



M08_40L_0105_0915

LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIRP



Consensus sequence

LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIR (SEQ ID NO: 373)











Acyl-coenzyme A synthetase alignments








C02_40L_0304_1021
             PEDEFFWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH


C02_40L_0604_1089
QTAKEIHFGEDQKPEDRFFWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH


C02_40L_0803_1137
                  FWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH


C02_40L_0505_1075
                  FWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH


Consensus sequence
                  FWVSDIGWMMGPWTLIGNHTFAGTIFMYEGAPDYPNPDRFWEMIERH (SEQ ID NO: 346)










hypothetical protein RSP_2990 alignments








M07_40L_0104_0882
  LLGVIVDGKEQTIIDDGNNEFGRKVSGDLDGTARFRWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


M07_40L_0104_0895
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHIPK


C03_40L_0104_0943
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


C03 40L_0104_0944
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


C03_40L_0104_0945
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


C03_40L_0104_0948
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHIRK


C03_40L_0104_0951
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


C03_40L_0104_0953
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


C03_40L_0105_0957
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHIHK


C03_40L_0105_0958
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


C03_40L_0105_0959
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


C03_40L_0105_0960
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


C03_40L_0105_0962
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILKG


C03_40L_0105_0964
                                     RWYLGNQTAADDYLLESYDEHPQFPWTTQHILK


C03_40L_0105_0965
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


C03_40L_0105_0966
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


C03_40L_0105_0967
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHIHK


C03_40L_0105_0969
ELLLGVIVDGKEQTIIDDGNNEFGRKVSGDLDGTARFRWYLGNQTAADDYLLESYGEHPQFPWTTQHILKGN


C03_40L_0105_0970
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHILK


Consensus sequence
                                     RWYLGNQTAADDYLLESYGEHPQFPWTTQHIXK (SEQ ID NO: 356)










unidentified protein alignment








C02_40L_0404_1047
            PHTEQDLFRFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTYcN


M09_40L_0205_1014
LIQSDIGNIcFTPHTEQDLFcFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTF


C02_40L_0104_0977
            PHTEQDLFRFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTY


C02_40L_0804_1154
LIQSDIGNIcFTPHTEQDLFRFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTF


Consensus sequence
            PHTEQDLF(R/C)FDSRSLPIFLYDDSVRFHFYYFcIQVESDSTY (SEQ ID NO: 374)










unidentified protein alignment








C02_40L_0804_1147

LIRWDRcVVGEGcDHLScSGLINNAHTNSITNLISNPFSGITLAcINPTSScFGIPFGARGRRN



C02_40L_0804_1156

LIRWDRcVVGEGcDHLScSGLINNAHTNSITNLISNPFSGITLAcINPTSScLGN



C02_40L_0705_1111

LIRWDRcVVGEGcDHLScSGLINNAHTNSITNLISNPFSGITLAcINPTSScL



C02_40L_0804_1163

LIRWDRcVVGEGcDHLScSGLINNAHTDSITNLISNPF



Consensus sequence

LIRWDRcVVGEGcDHLScSGLINNAHT(D/N)SITNLISNPF (SEQ ID NO: 375)











Type IV secretion system protein pt1C (pertussis toxin liberation protein C) alignments








C02_40L_0304_1030

LWWVFDNPNDcLDFSRPGKYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTIRKKNGLGVFSTQMPSSLL



C02_40L_0604_1084

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTIRKKNGLGVFSTQMP



C02_40L_0704_1103

LWWVFDNPNDcLDFSRPGNYGIDGTAELDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTI



C02_40L_0704_1106

LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTT



C02_40L_0704_1104

LWWVFDNPNDcLDFSRPGNYGIDGTAELDNAETRTPISTYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFA



M09_40L_0103_0757
WWVFDNPNDCLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTI


C02_40L_0604_1086
LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTI


C02_40L_0604_1081
LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHAMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTI


C02_40L_0504_1061
LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTI


C02_40L_0404_1043
LWWVEDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRREVYLMDEAWKWIDDPAFAEFAGDQQLTI


C02_40L_0304_1022
LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTI


M09_40L_0105_0928
LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTI


M09_40L_0105_0923
LWWVFDNPNDcLDFSRPGNYGIDGTAFLDNAETRTPISMYLLHRMNEAMDGRRFVYLMDEAWKWIDDPAFAEFAGDQQLTI


Consensus sequence
 WWVFDNPNDcLDFSRPG(K/N)YGIDGTAFLDNAETRTPISMYLLHRM(N/S)EAMDGRRFVYLMDEAWKWIDDPAFAEFA (SEQ ID NO: 347)










putative uncharacterized protein alignment








C02_40L_0103_0972
                                 LYDYPDRSGPWWDAVFYEGNSLQYPSFVPQDVAGLMANTGGPDGFVKWLDHLFDGHYSQSNEPDLLAPYLYIQRNSc


M08_40L_0104_0743
LARSGNWKNLWDDAIGcVRPRYPNGEWVENYScTYDYPDRSGPWWDAVFYEGNSLQYSSFVPQDVAGLMANTGGPDGFVKWLDHLFDGHYSQ


M09_40L_0103_0764
         LWDDAIGcVRPRYPNGEWVENYScTYDYPDRSGPWWDAVFYEGDSLQYSSFVPQDVAGLMANTGGPDGFVKWLDHLFDGHYSQ


Consensus sequence
                                  YDYPDRSGPWWDAVFYEG(N/D)SLQY(P/S)SFVPQDVAGLMANTGGPDGFVKWLDHLFDGHYSQ (SEQ ID NO: 376)










ATP-dependent helicase alignment








M08_40L_0104_0738
HFKPKOLLGLTATPEWMDGLNVQDKFFEGRIAAELRLWEALENDLLCPFHYFGIPDGTDL


C02_40L 0204_0990
 FKPKQLLGLTATPERMDGLNVQDEFFEGRIAAELRLWEALENDLLCPFHYFGIPDGTDLT


M06_40L_0104_0819
 FKPKQLLGLTATPERMDGLNVQDEFFEGRIAAELRLWEALENDLLCPFHYFGIPDGTDLT


Consensus sequence
 FKPKQLLGLTATPE(W/R)MDGLNVQD(K/E)FFEGRIAAELRLWEALENDLLCPFHYFGIPDGTDL (SEQ ID NO: 348)










putative dioxygenase alpha subunit YeaW alignments








M09_40L_0203_1004
                 LcEYEHAARYVSEVEcNWKTFAGNYSEcDHcHANHQDWITDIELAESELEVNDYHWILHcTHDEDVEDEMRIHDEHEAKFYYFWPNFN


M09_40L_0104_0774
                PLGEYEHAARYVSEVEcNWKTFAGNYSEcDHcHANHQDWITDIELAESELEVNDYHWILHYTHDEDVEDEMRIHDEHEAKFYYFWPNFT


M09_40L_0104_0789
LLAEQAGTLKSELEAMPLGEYEHAARYVSEVEcNWKTFAGNYSEcDHcHANHQDWITDIELEESELEVNDYHWILHYTHDEDVEDEMRIHDEHEAKFYYFWPNFT


M09_40L_0104_0767
                 LGEYEHAARYVSEVEcNWKTFAGNYSEcDHcHANHQDWITDIELAEPELEVNDYHWILHYTHDEDVEDEMRIHDEHEAKFYYFWPNFT


Consensus sequence
             L(C/G)EYEHAARYVSEVECNWKTFAGNYSECDHCHANHQDWITDIEL(A/E)E(S/P)ELEVNDYHWILH(C/Y)THDEDVEDEMRIHDEHEAKFYYFWPNF



(SEQ ID NO: 349)










putative Alpha amylase alignments








M09_40L_0104_0791
LAYGKSTEDKQDFLLFHVNLDPHAAQTFEFEVPLWEFGLPDDASVEVEDLLNGNRFTWHGKWQWLELDPQTRPYAVWRLYAPGMPR


C02_40L_0803_1126
LAYGKSTEDKQDFLLFHVNLDPHAAQTFEFEVPLWEFGLPDDASVEVEDLLNGNRFTWHGKWQWLELDPQT


C02_40L_0204_0994
LAYGKSTEDKQDFLLFHVNLDPHAAQTLEFEVPLWGFGLPDDASVEVEDLLNGDRFTWHGKWQWLELDPQT


Consensus sequence
LAYGKSTEDKQDFLLFHVNLDPHAAQT(F/L)EFEVPLW(E/G)FGLPDDASVEVEDLLNG(N/D)RFTWHGKWQWLELDPQT (SEQ ID NO: 350)










putative outer membrane protein alignment








C02_40L_0104_0974

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ



C02_40L_0105_0982

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ



C02_40L_0105_0984

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ



C02_40L_0305_1033

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ



C02_40L_0305_1034

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ



C02_40L_0305_1035

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGNTWGDLFIFFDQ



C02_40L_0404_1041

QPVPERRLLLGDHPQODRHSDQQTFTLEHASGWTWGDLFIFFDQ



C02_40L_0705_1113

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ



Consensus sequence

QPVPERRLLLGDHPQGDRHSDQQTFTLEHASGWTWGDLFIFFDQ (SEQ ID NO: 351)











putative haemagglutinin alignments








C02_40L_0305_1032
RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSWGHYLNAYDAFPGHNR


C02_40L_0504_1057
     QVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSWGHYLNAYDAFPDYN


C02_40L_0505_1074
RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSWGHYLNAYDAFPGHNR


C02_40L_0505_1076
RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSWGHYLNAYDAFPGHNR


C02_40L_0605_1094
RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSWGHYLNAYDAFPGHNR


C02_40L_0705_1110
RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSWGHYLNAYDAFPGHNR


C02_40L_0804_1171
RYYPLQVEYcVTAVYDESIESSTVcGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSWGHYLNAYDAFPGHNR


Consensus sequence
RYYPLQVEYcVTAVYDESIESSTVCGTLHYATDAILYENFENGPVPNGWLVIDADGDGFSWGHYLNAYDAFP(G/D)(H/Y)NR (SEQ ID NO: 352).










putative terminase large subunit alignments








M06_40L_0103_0810
LKEIADNANVQKVAFDRYKIKYFKRDMIDcGFDERWIDEHMVSYGQGFEKV


M06_40L-0104-0837
LKEIADNANVQKVAFDRYKIKYFKRDMIDCGFDERWIDEHMVSYGQGFVSMG


Consensus sequence
LKEIADNANVQKVAFDRYKIKYFKRDMIDCGFDERWIDEHMVSYGQGF (SEQ ID NO: 353)










unidentified protein alignment








M06_40L_0104_0822
                  PFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT


M06_40L_0104_0830
                  PFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT


M07_40L_0103_0860
                  PFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT


M07_40L_0104_0864
LSGGPDHVPHPEHSTYDFPFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT


M07_40L_0104_0866
        PQPEHSTYDFPFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT


M07_40L_0104_0879
LGGGPDHVPHPEHSTYDFPFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT


M07_40L_0104_0884
                  PFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRT


M07_40L_0104_0896
LGGGPDHVPHPEHSTYDFPFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTRM


Consensus sequence
                  PFPDGSScAPPGWLGGVPcFLQRYMLHQPDARGTLEPTR(M/T) (SEQ ID NO: 377)










hypothetical protein SAV_ 4481 alignment








M06_40L_0104_0825
HKEYNYWEKHKDDKYYYWNTYKEYNYWEKHKDDKcYWNEKDTK


M08_40L_0105_0903
YKEYNYWE--------------------KHKDDKCYW


MO8_40L_0105_0904
YKEYNYWEKHKDD--------------------KCYWNEKDTKN


M08_40L_0105_0913
RYYFEEHcScYYYTENDHNcYWDDHYNSYYVVQYNHKYYWDYHYDcYYVVEKH


M09_40L_0105_0918
YKEYNYWE--------------------KHKDDKCYWN


M09_40L_0105_0922
YKEYNYWE--------------------KHKDDKCYWNDGSTR


M09_40L_0105_0924
YKEYNYWEKHKDD--------------------KCYWNEKDTKN


M09_40L_0105_0930
RCYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKHCKYYYWNTYKEYNYWEKHKDDKYIEGTEKVVTNAAI


M09_40L_0105_0934
 CYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKHCKYYYWNTYKEYNYWEKHKDDK


M09_40L_0104_0788
 CYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKH


M07_40L_0103_0847
   HYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKHC


M08_40L_0105_0898
 CYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKH


M09_40L_0104_0790
 CYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKQC


M08_40L_0105_0900
       SSHNYCWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKH


M09_40L_0105_0927
RCYYYTENDHNYYWDDHYNSYYVVQYNHKYYWDYHYDCYYVVEKHC


Consensus sequence
         HNY(Y/C)WDDHYNSYYVVQYNHKYYWDYHYDCYYVVEK (SEQ ID NO: 357)










unidentified protein alignment








M06_40L_0104_0829
                                                    LSDYEcPPVFLSGGDVPRcWVAVRGFEFFGRDFRcGEV


M06_40L_0104_0840
                                           PGQFQLTRVFSDYEcPPVFLSGGDVPRcWVAVRGFEFFGRDFRcGEV


C02_40L_0605_1092
PGVDLGVQLLFQTVEcGDGVSGVSWFDELSYVDFAIDAEKFQGPGQFQLTRVFGDYEcPPVFLSGGDVPRcWVAVRGFEFFS


Consensus sequence
                                                      DYEcPPVELSGGDVPRcWVAVRGFEFF (SEQ ID NO: 378)










unidentified protein alignnient








M06_40L_0103_0805
RWDRPWYSPVTNWSPHCRGLD


M06_40L_0104-0835
 WDRPWYSPVTNWSPHCRGLD


Consensus sequence
 WDRPWYSPVTNWSPHCRGLD (SEQ ID NO: 379)










unidentified protein alignment








M06_40L_0103_0811
RGTSWGTACPWWFPTTTALTR


M06_40L_0104_0826
RGTSWGTACPWWFPTTTALTR


Consensus sequence
RGTSWGTACPWWFPTTTALTR (SEQ ID NO: 380)










unidentified protein alignment








M06_40L_0103_0812
LSCSWCSFFPDTKSVSCQD


M06_40L-0104_0838
LSCSWCSFFPDTKSVSCQD


Consensus sequence
LSCSWCSFFPDTKSVSCQD (SEQ ID NO: 381)










unidentified protein alignment








M09_40L_0104_0785
LWDVKTYVSDQDGTGWDLVEQYQNKYGMPNPDGTIGKTLWLDYIQ


M07_40L_0104_0874
LWDVKTYVSDQDGTGWDLVEQYQNKYGMPNPDGTIGKTLWLDYIQ


Consensus sequence
LWDVKTYVSDQDGTGWDLVEQYQNKYGMPNPDGTIGKTLWLDYIQ (SEQ ID NO: 382)










unidentified protein alignment








M07_40L_0104_0883
                            RCEDQVDVIAGHRPGDFLLRATLAMDPRVKPADDGGGWSWWFL


M07_40L_0104_0885
REVLQLDDHVHVSPALAVQELLAKPHVGRRAVSVDVIAGHRPGDFLLRATLAMDPRVKPADDGGGWSWWFI


Consensus sequence
                                 VDVIAGHRPGDFLLRATLAMDPRVKPADDGGGWSWWF (SEQ ID NO: 383)










unidentified protein alignment








M07_40L_0104_0889



C02_40L 0803_1123
LWWLcYQDDEISTEATNEIGLPKYGTDEKQDALRKYYAAYFNVEGGDSGTFTDYKWDcFWAHRHAIMH


C02_40L_0803_1120
LWWLcYQDDEISTEATNEIGLPKYGTDEKQDALRKYYAAYFNVEGGDSGTFTDYKWDcFWAHRHAIMH


C02_40L_0803_1145



Consensus sequence
LWWLcYQDDEISTEATNEIGLPKYGTDEKQDALRKYYAAYFNVEGGDSGTFTDYKWDcFWAHRHAIMH (SEQ ID NO: 384)










unidentified protein alignment








M08_40L_0103_0717
LHLPRPGFCDARSHGDSNFEDLFYFILCGTH


C03_40L_0103_0942
 HLPRPGFCDARSHGDSNFEDLFYFILCGTH


M08_40L_0103_0723
 HLPRPGFCDARSHGDSNFEDLFYFILCGTH


Consensus sequence
 HLPRPGFCDARSHGDSNFEDLFYFILCGTH (SEQ ID NO: 385)










hypothetical protein DR_A0144 alignment








M08_40L_0103_0719
RDGNFDDTDRVGTVHDMRFVFLDNDTKLLFCTAYDDEWDPYIDDFATKIPDELDLFK


M09_40L_0103_0753
LRDGNFDDTDRVGTVHDMRFVFLDNDTKLLFcTAYDDEWDPYIDDFATKIPDELDLFI


Consensus sequence
 RDGNFDDTDRVGTVHDMRFVFLDNDTKLLFCTAYDDEWDPYIDDFATKIPDELDLF (SEQ ID NO: 358)










hypothetical protein GSU1508 alignment








M08_40L_0103_0720
RLPETRKAQAALATKYGIYGFCYYHYWFNGRRILESPVDAMLESGEPDFPFMLCWANENWT


C02_40L_0504_1062
RIPETRKAQAALATKYGIYGFCYYHYWFNGRRILESPVDAMLESGEPDFPFMLCWANENWT


Consensus sequence
R(L/I)PETRKAQAALATKYGIYGFCYYHYWFNGRRILESPVDAMLESGEPDFPFMLCWANENWT (SEQ ID NO: 359)










hypothetical protein pNG7041 alignment








M08_40L_0103_0725
                  LWNDGVSKEPFYEMDADLHLIDPNALIDWLGAWDQSDLTEIRENVAPFLGNLIFRQTDDWHDYRYY


M08_40L_0104_0737
LPYEELDGVSIDLOGLTQLWNDGVSKEPFYEMDADLHLIDPNALIDWLGAWDQSDLT


Consensus sequence
                  LWNDGVSKEPFYEMDADLHLIDPNALIDWLGAWDQSDLT (SEQ ID NO: 360)










hypothetical protein SAV_2940 alignment








M08_40L_0104_0739
LLQGEDMIEQLGRAHMSWGLGSADRWDLDQTTGIITWTFPDKTAAAPAQILGSFSPGSGSWLWAWANK


M08_40L_0105_0902
 LLGEDMIEQLGRAHMSWGLGSADRWDLDQTTGIITWTFPDKTAAAPAQILGSFSPGSGSWLWAWANK


Consensus sequence
L(L/Q)GEDMIEQLGRAHMSWGLGSADRWDLDQTTGIITWTFPDKTAAAPAQILGSFSPGSGSWLWAWANK (SEQ ID NO: 361)










hypothetical protein PA2G_00938 alignment








M08_40L_0105_0909
LAEHAVWSLKCFPDWEWYNINIFGTDDPNHFWVECDGHGKILFPGYPEGYYENHFLHSFELED


M08_40L_0104_0747
LAEHAVWSLKCFPDWEWYNINIFGTDDPNHFWVECDGHGKILFPGYPEGYYENHFLHSFELED


Consensus sequence
LAEHAVWSLKCFPDWEWYNINIFGTDDPNHFWVECDGHGKILFPGYPEGYYENHFLHSFELED (SEQ ID NO: 362)










hypothetical protein SAV_5325 alignment








C02_40L_0205_0998
LGHIWASDVENAASFEPVDVGDEEAYKAGLLWLERLTMSDNGLRQLALFEWDEDGNLTKEWHAE


M08_40L_0105_0910
LGHIWASDVENAASFEPVDVGDEEAYKAGLLWLERLTMSDNGLRQLALFEWDEDGNLTKEWHAE


Consensus sequence
LGHIWASDVENAASFEPVDVGDEEAYKAGLLWLERLTMSDNGLRQLALFEWDEDGNLTKEWHAE (SEQ ID NO: 363)










unidentified protein alignment








M09_40L_0103_0760
RWLVGTYSYQNDAYRQLFEPDDESALLQELSEYLDDHGSEPIIYYGGNYFDEQCLSRRFDEH


M08_40L_0105_0912
  LVCTYSYQNDAYRQFFEPDDESALLQELSEYLDDHGSEPIIHYGGNYFDEQCLSRRFDE


Consensus sequence
  LV(G/C)TYSYQNDAYRQ(L/F)FEPDDESALLQELSEYLDDHGSEPII(H/Y)YGGNYFDEQCLSRRFDE (SEQ ID NO: 386)










hypothetical protein CT1305 alignment








M08_40L_0105_0915
LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIRP


C02_40L_0304_1027
LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIR


C02_40L_0205_0996
LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIRLGNFYFIYRDGYWF


Consensus sequence
LLGTPQNNAQAEVNLNINIGGPRYVGNYGPDFIYLDDYGFAVSWGWDYDVIR (SEQ ID NO: 364)










unidentified protein alignment








M09_401_0103_0762
                QRLAERYLSESYWGDVIEASDDVWELVACPVDG


M09_40L_0204_1006
LGDINPLKRIVDSRQSKRLAERYLSESYWGDVIEASDDVWELVACPVDGALDAALWDAWLESLEEG


Consensus sequence
                 RLAERYLSESYWGDVIEASDDVWELVACPVDG (SEQ ID NO: 387)










putative modification methylase alignment








M09_40L_0104_0775
LDLFGDFNGLPEGADRTEFYQHEGHWQNRMILGDSLQVMASLAEREGLRGKVQCIYFDPPYGIKFN


M09_40L_0103_0763
LDLFGDFNGLPEGADRTEFYQHEGHWQNRMILGDSLQVMASLAEREGLRGKVQCIYFDPPYGIKFN


Consensus sequence
LDLFGDFNGLPEGADRTEFYQHEGHWQNRMILGDSLQVMASLAEREGLRGKVQCIYFDPPYGIKFN (SEQ ID NO: 354)










hypothetical protein TGME49_103250 alignments








C02_40L_0603_1077
                   LWIGINTNGMQWNGMQWQRMEWNGMEWHKPEWYGMEWNGMEWNGMEWKGIE


M09_40L_0105_0935
                   LWIGINTNGMQWNGMQWQRMEWNGMEWHKPEWYGMEWNGMEWNGMEWKGIE


M09_40L_0105_0931
                   LWIGINTNGMQWNGMQWQRMEWNGMEWHKPEWYGMEWNGMEWNGMEWKGIE


M09_40L_0104_0784
                   LWIGINTNGMQWNGMQWQRMEWNGMEWHKPEWYGMEWNGMEWNGMEWKGIE


C03_40L_0203_1175
 PRMEWNVLQWNGMESNELVSNGTEWNGMDWNAMEWNRMEWNGMEWNQSEWNGRELNGMEWKGMEWNGMEWNGTNPSGME


M09_40L_0204_1010
RNEVEWNGMERNGMEWSGMELNGTQWNEVEWSRMEWNGWMWNGMEWNGMEWNGEEWSGVE


M07_40L_0104_0893
                   LWNGIIRNGMERNGMEWNGMEWNGMEWNGMEWVRIEWNGMDSNGIAWNGMDSNAMERNALEWNGMDSKAME . . .


M09_40L_0204_1005
                             PWNEMEWKGIEWNQPEWNGMERNGMEWNGMEWNGMEWNQLDWNGMEWNGLE


C02_40L_0104_0979
               PWNEMEWKGIEWNQPEWNGMERNGMEWNGMEWNGMEWNQLDWNGMEWNGLE


C02_40L_0804_1169
RLEWNGMELNGTTPSEMAWKGTEYNLMEWNGINPSGMEWIGMEWNGMEWKGMEWNGMEWFQLE


M06_40L_0104_0839
        PGVVRScVEWSGIDWScEELcGVEWNGVEWKGVEWNGMEWNGMELNGREWSGTEENGVEWSGVERSGSW


C02_40L_0705_1114
                        LWcEWELEWNGMEWNGMEWNGMEWNAMEcNGFNSIAMEWNAMEWNQPE


Consensus sequence
                                  (G/R)(M/W)EWNGME(W/L)(N/K)(G/Q)XEW (SEQ ID NO: 365)










unidentified protein alignment








M09_40L_0205_1016
LSLYCRNHRVECFCCHTGGDRSELPSTHYSTSSGFMQVYDFFGVPFVLEY


M09_40L_0105_0933
LSLYCRNHRVECFCCHTGGDRSELPSTHYSTSSGFMQVYDFFGVPFVLEY


Consensus sequence
LSLYCRNHRVECFCCHTGGDRSELPSTHYSTSSGFMQVYDFFGVPFVLEY (SEQ ID NO: 388)










unidentified protein alignment








C02_40L_0104_0977
            PHTEQDLFREDSRSLPIFLYDDSVRFHFYYFcIQVESDSTY


M09_40L_0205_1014
LIQSDIGNIcFTPHTEQDLFcFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTF


C02_40L_0404_1047
            PHTEQDLFRFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTY


C02_40L_0804_1154
LIQSDIGNIcFTPHTEQDLFRFDSRSLPIFLYDDSVRFHFYYFcIQVESDSTF


Consensus sequence
            PHTEQDLF(R/C)FDSRSLPIFLYDDSVRFHFYYFcIQVESDST (SEQ ID NO: 389)










transposase family protein [Halogeometricum borinquense DSM 11551] alignment








C02_40L_0105_0983
QVLRTLQVVTcRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN


C02_40L_0105_0985
QVLRTLQVVTcRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN


C02_40L_0105_0986
QVLRTLQVVTcRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN


C02_40L_0105_0987
QVLRTLQVVTcRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN


Consensus sequence
QVLRTLQVVTCRAGELEIRLETLEDGYPEWHPASYPFQGILKLFFYREITGN (SEQ ID NO: 355)










unidentified protein alignment








M09_40L_0104_0781
            LLERLNGVSVDWSNLYNSWNPDKETLYELDSDLHLADPAYVSTMDAWDTADVEEVQTEIAPWFGNSLSRNHSEPPADWADQYQYYSLWDIY


C02_40L_0203_0988
LWWDGTVAGLEYFTAGFDGFEIEWADAAGEYGFTKERLYELDSDLHLVDPVWVTMQDNWNRSDTDEVADNIGPWFGNYY


Consensus sequence
                              KE(T/R)LYELDSDLHL(A/V)DP (SEQ ID NO: 390)










hypothetical protein Hlac_3130 alignment








C02_40L_0204_0992
LLGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWcPVcGREVESHIPFEGVFcK


C02_40L_0505_1072
 LGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWcPVcGHEVESHIPFEGVFc


Consensus sequence 
 LGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWCPVCGHEVFSHIPFEGVFC (SEQ ID NO: 366)










hypothetical protein pNG6140 alignment








C02_40L_0204_0992
LLGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWcPVcGHEVFSHIPFEGVFcK


C02_40L_0505_1072
 LGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWcPVcGHEVFSHIPFEGVFc


Consensus sequence
 LGRDGWPVSIGPDSQMSLEVIDRHSEALFEFLWCPVCGHEVFSHIPFEGVFC (SEQ ID NO: 367)










unidentified protein alignment








C02_40L_0303_1020
LFGSRPLSRScLELLHEESEISVEAVVTHPEGHDGWWDGALRSKAEEYGYPVIEENEVFEcELDYIISVLYYEILDAELLEHPKQGGLNLHQAELPRYRG


C02_40L_0503_1053
LFGSRPLSRScLELLHEESEISVEAVVTHPEGHDGWWDGALRPKAEEYGYPVIEENEVFEcELDYIISVLYYEILDAELLEHPKQGGLNLHQAELPRYRG


C02_40L_0704_1100
LFGSRPLSRScLELLHEESEISVEAVVTHPEGHDGWWDGALRSKAEEYGYPVIEENEVFEcELDYIISVLYYEILDAELLEHPKQGGLNLHQAELPRYRG


Coneensus sequence
LFGSRPLSRScLELLHEESEISVEAVVTHPEGHDGWWDGALR(S/P)KAEEYGYPVIEENEVFEcELDYIISVLYYEILDAELLEHPKQGGLNLHQAELPHYRG (SEQ ID NO: 391)










unidentified protein alignment








C02_40L_0304_1028
LcDPNGRAEAIPEAKSDVTVTHQFITIWSEWIRMDVLMTGVYGRcGTAVIDHLHDDDAYDFTYLN


C02_40L_0404_1045
LGDPNGRAEAIPEAKSDVTVTHQFITIWSEWIRMDVLMTGVYGRcGTAVIDHLHDDDAYDFTYFN


Consensus sequence
  DPNGRAEAIPEAKSDVTVTHQFITIWSEWIRMDVLMTGVYGRcGTAVIDHLHDDDAYDFTY (SEQ ID NO: 392)










hypothetical protein CC_2361








C02_40L_0405_1048
LYEYNADTPTSIYEAGVFQWLWLEDMIQQGALPEATDQFNSLHDQLAERFKAIFPNGGFVHFA


C02_40L_0604_1080
LYEYNADTPTSIYEAGVFQWLWLEDMIQQGALPEATDQFNSLHDQLAERFKAIFPN


Consensus sequence
LYEYNADTPTSIYEAGVFQWLWLEDMIQQGALPEATDQFNSLHDQLAERFKAIFPN (SEQ ID NO: 368)










unidentified protein alignment








C02_40L_0803_1122
PVEKLNNLTGTPLVASFTLFVcQSGQNTLLEcTLIV


C02_40L_0804_1172
PVEKLNNLTGTPLVASFTLFVcQSGQNTLLEcTLIV


Consensus sequence
PVEKLNNLTGTPLVASFTLFVcQSGQNTLLEcTLIV (SEQ ID NO: 393)










unidentified protein alignment








C02_40L_0803_1125
LSAcTPSGcTHPGSRGDDLQMATOWIFTLRcRTVGNSTRDVRISTOGSPHIELIDPFGNGALWVIL


C02_40L_0803_1127
LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGALWVIL


C02_40L_0804_1148
LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGALWVIL


C02_40L_0804_1149
LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGALWVIL


C02_40L_0804_1152
LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGALWVIL


C02_40L_0804_1155
LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGALWVIL


C02_40L_0804_1162
LSAcTPSGeTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGALWVIL


C02_40L_0804_1164
LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGALWVIL


Consensus sequence
LSAcTPSGcTHPGSRGDDLQMATQWIFTLRcRTVGNSTRDVRISTQGSPHIELIDPFGNGALWVIL (SEQ ID NO: 394)










hypothetical protein PH1675 alignment








C02_40L_0804_1151
LVQGSKEATTIDESAELEALADAVAEEILSSDGIYFLGAGSTIKRIKDKIGIEGTLLGVDVVRVENGKAKLLVKDA


C02_40L_0804_1173
LVQGSKEATTIDESAELEALADAVAEEILSSDGIYFLGAGSTIKRIKDKIGIEGTLLGVDVVRVENGKAKLLVKDA


Consensus sequence
LVQGSKEATTIDESAELEALADAVAEEILSSDGIYFLGAGSTIKRIKDKIGIEGTLLGVDVVRVENGKAKLLVKDA (SEQ ID NO: 369)










Gifsy-1 prophage protein alignment








C02_40L_0804_1157
LVcGWTDEDEIGLFVQVGAILRGESEITWGEPLYLSGVVT


C02_40L_0804_1165
LVcGWTDEDEIGLFVQVGAILRGESEITWGEPLYLSGVVT


Consensus sequence
LVCGWTDEDEIGLFVQVGAILRGESEITWGEPLYLSGVVT (SEQ ID NO: 370)








Claims
  • 1. A composition comprising one or more peptides, analogs or derivatives, wherein a peptide, analog or derivative of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, and wherein said peptide, analog or derivative comprises a secondary structure or assembly of secondary structures of a protein, or portion thereof, comprising an amino acid sequence that is substantially homologous and/or aligns to a consensus domain comprised in two or more amino acid sequences set forth in Table 10.
  • 2-163. (canceled)
  • 164. A composition comprising one or more peptides, analogs or derivatives, wherein a peptide, analog or derivative of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, and wherein said peptide, analog or derivative forms a secondary structure or assembly of secondary structures comprising an anti-parallel beta sheet.
  • 165-171. (canceled)
  • 172. A composition comprising one or more peptides, analogs or derivatives, wherein a peptide, analog or derivative of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, and wherein said peptide, analog or derivative comprises a secondary structure or assembly of secondary structures comprises an alpha helix.
  • 173-175. (canceled)
  • 176. A composition comprising one or more peptides, analogs or derivatives, wherein a peptide, analog or derivative of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, and wherein said peptide, analog or derivative comprises a secondary structure or assembly of secondary structures identifiable, determinable or predictable from an amino acid sequence selected from those set forth in Table 8, or a consensus domain amino acid sequence selected from those set forth in Table 10.
  • 177. (canceled)
  • 178. A composition comprising one or more peptides, analogs or derivatives, wherein a peptide, analog or derivative of the composition comprises a sequence of amino acids other than a sequence of CD40, wherein the peptide, analog or derivative binds to CD40 ligand (CD40L) and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects, and wherein said peptide, analog or derivative comprises a primary amino acid sequence selected from those amino acid sequences set forth in Table 8, or a consensus domain amino acid sequence selected from those set forth in Table 10.
  • 179. The composition according to claim 1, wherein the peptide, analog or derivative that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects comprises a sequence encoded by a nucleic acid fragment of a prokaryote genome or a compact eukaryote genome.
  • 180. The composition according to claim 1, wherein the peptide, analog or derivative comprises a sequence of a natural open reading frame of a prokaryote genome or a compact eukaryote genome.
  • 181. The composition according to claim 1, wherein the peptide, analog or derivative that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects does not comprise N-terminal and C-terminal cysteine residues for achieving conformational stability.
  • 182. The composition according to claim 1, wherein the peptide, analog or derivative that binds CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects is cysteine-free.
  • 183. The composition according to claim 1, wherein the peptide, analog or derivative that binds CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects comprises one or more D amino acids.
  • 184. The composition according to claim 1, wherein the peptide, analog or derivative that binds CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects is a retroinverso peptide analog.
  • 185. The composition according to claim 1, wherein the peptide, analog or derivative that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects is a peptidyl-fusion between a plurality of smaller peptides that each bind CD40L, wherein the peptidyl-fusion has a higher affinity for CD40L and/or enhanced inhibitory activity than a single peptide of the peptidyl-fusion.
  • 186. The composition according to claim 185, wherein the peptidyl-fusion is a dimer comprising two peptides that each bind CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects.
  • 187. The composition according to claim 1, wherein the peptide, analog or derivative that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects is a peptidyl-fusion between the peptide that binds CD40L and a serum protein-binding moiety or serum protein moiety.
  • 188. The composition according to claim 1, wherein the peptide, analog or derivative that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects is a peptidyl-fusion between the peptide that binds CD40L and a protein transduction domain.
  • 189. The composition according to claim 1, wherein the peptide, analog or derivative that binds to CD40L and partially or completely inhibits interaction of CD40 with CD40L and/or one or more CD40-CD40L costimulatory effects comprises a polyethylene glycol (PEG) moiety, a hydroxyetheyl starch (HES) moiety, or a polyglycine moiety.
  • 190. The composition according to claim 1 comprising a pharmaceutically acceptable carrier and/or excipient.
  • 191-208. (canceled)
  • 209. A method of preventing or treating one or more adverse consequences of CD40L-dependent signaling in a subject, said method comprising administering an amount of the composition according to claim 1 for a time and under conditions sufficient to inhibit inappropriate CD40L-dependent signaling.
  • 210. A method of preventing or treating inflammation in a subject, said method comprising administering an amount of the composition according to claim 1 for a time and under conditions sufficient to ameliorate one or more adverse effects of CD40L-dependent signaling that contribute to an inflammatory response in a subject.
  • 211. A method of preventing or treating autoimmunity in a subject, said method comprising administering an amount of the composition according to claim 1 for a time and under conditions sufficient to ameliorate one or more adverse effects of CD40L-dependent signaling that contribute to autoimmunity in a subject.
  • 212. A method of preventing or treating cancer or metastatic disease in a subject, said method comprising administering an amount of the composition according to claim 1 for a time and under conditions sufficient to ameliorate one or more adverse effects of CD40L-dependent signaling that contribute to cancer in a subject.
  • 213. A method of treatment of a disease or condition, said method comprising administering an amount of the composition according to claim 1 for a time and under conditions sufficient to attenuate or reduce humoral immunity against a therapeutic protein administered to the subject for treatment or prevention of the disease or condition.
  • 214. (canceled)
  • 215. A method of treating a viral infection in a subject, said method comprising administering an amount of the composition according to claim 1 for a time and under conditions sufficient to attenuate or reduce humoral immunity against a cytokine administered to the subject.
  • 216. A method of treating hemophilia, said method comprising administering an amount of the composition according to claim 1 for a time and under conditions sufficient to attenuate or reduce humoral immunity against a clotting factor administered to the subject.
  • 217. A method of identifying or determining or predicting a secondary structure of a peptidyl inhibitor of an interaction of CD40 with CD40L, wherein said method comprises aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit said interaction to the primary sequence(s) of one or more known proteins or fragment(s) thereof, determining a secondary structure for the known protein(s) or fragment(s), and assigning the secondary structure for the one or more known protein(s) or fragment(s) to the one or more peptides, analogs or derivatives.
  • 218-383. (canceled)
  • 384. A method of identifying or determining or predicting a secondary structure of a peptidyl inhibitor of an interaction of CD40 with CD40L, wherein said method comprises aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit said interaction to the primary sequence(s) of one or more known proteins or fragment(s) thereof, determining a secondary structure for the known protein(s) or fragment(s), and assigning the secondary structure for the known protein(s) or fragment(s) to the one or more peptides, analogs or derivatives, wherein said peptide, analog or derivative forms a secondary structure or assembly of secondary structures comprising an anti-parallel beta sheet.
  • 385-391. (canceled)
  • 392. A method of identifying or determining or predicting a secondary structure of a peptidyl inhibitor of an interaction of CD40 with CD40L, wherein said method comprises aligning primary sequence(s) of one or more peptides, analogs or derivatives that inhibit said interaction to the primary sequence(s) of one or more known proteins or fragment(s) thereof, determining a secondary structure for the known protein(s) or fragment(s), and assigning the secondary structure for the known protein(s) or fragment(s) to the one or more peptides, analogs or derivatives, wherein said peptide, analog or derivative comprises a secondary structure or assembly of secondary structures comprising an alpha helix.
  • 393-403. (canceled)
CROSS REFERENCE TO RELATED APPLICATION

This application claims priority to U.S. application No. 61/383,447 filed Sep. 16, 2010 the full contents of which is incorporated by reference herein.

PCT Information
Filing Document Filing Date Country Kind 371c Date
PCT/AU2011/001198 9/16/2011 WO 00 6/17/2013
Provisional Applications (1)
Number Date Country
61383447 Sep 2010 US