Claims
- 1. An isolated polynucleotide that encodes a fusion protein comprising a first amino acid sequence and a second amino acid sequence, wherein said first amino acid sequence is the amino acid sequence of an approximately 45 kDa polypeptide and said second amino acid sequence is the amino acid sequence of an approximately 15 kDa polypeptide, wherein said polypeptides are toxic to a rootworm pest that ingests said polypeptides, wherein a nucleotide sequence that codes for said first amino acid sequence hybridizes with the complement of the nucleic acid sequence of SEQ ID NO:10, and wherein a nucleotide sequence that codes for said second amino acid sequence hybridizes with the complement of the nucleic acid sequence of SEQ ID NO:31.
- 2. An isolated polynucleotide that encodes a fusion protein comprising a first amino acid sequence and a second amino acid sequence wherein said fusion protein is toxic to a rootworm pest that ingests said protein; wherein said first amino sequence is the amino sequence of an approximately 45 kDa polypeptide and said second amino acid sequence is the amino acid sequence of an approximately 15 kDa polypeptide; wherein a nucleotide sequence that codes for said first amino acid sequence hybridizes under stringent conditions with the complement of a nucleic acid sequence selected form the group consisting of SEQ ID NO:10, SEQ ID NO:42, and SEQ ID NO:45; and wherein a nucleotide sequences that codes for said second amino acid sequence hybridizes under stringent condition with the complement of nucleic acid sequence selected from the group consisting of SEQ ID NO:31, SEQ ID NO:40, and SEQ ID NO:44.
- 3. The polynucleotide according to claim 1 wherein said polynucleotide encodes a fusion protein comprising the amino acid sequence of SEQ ID NO:159.
- 4. The polynucleotide according to claim 1 wherein said polynucleotide comprises the nucleic acid sequence of SEQ ID NO:160.
- 5. The polynucleotide according to claim 2 wherein a nucleotide sequence that codes for said first amino acid sequence hybridizes under stringent conditions with the complement of the nucleic acid sequence of SEQ ID NO:10.
- 6. The polynucleotide according to claim 2 wherein a nucleotide sequence that codes for said second amino acid sequence hybridizes under stringent conditions with the complement of the nucleic acid sequence of SEQ ID NO:31.
- 7. The polynucleotide according to claim 2 wherein a nucleotide sequence that codes for said first amino acid sequence hybridizes under stringent conditions with the complement of the nucleic acid sequence of SEQ ID NO:42.
- 8. The polynucleotide according to claim 2 wherein a nucleotide sequence that codes for said second amino acid sequence hybridizes under stringent conditions with the complement of the nucleic acid sequence of SEQ ID NO:40.
- 9. The polynucleotide according to claim 2 wherein a nucleotide sequence that codes for said first amino acid sequence hybridizes under stringent conditions with the complement of the nucleic acid sequence SEQ ID NO:45.
- 10. The polynucleotide according to claim 2 wherein a nucleotide sequence that codes for said amino acid sequence hybridizes under stringent conditions with the complement of the nucleic acid sequence of SEQ ID NO:44.
- 11. The polynucleotide according to claim 2 wherein said polynucleotide comprises the nucleic acid sequence of SEQ ID NO:44.
- 12. The polynucleotide according of claim 2 wherein said polynucleotide comprises the nucleic acid sequence of SEQ ID NO:45.
- 13. The polynucleotide according to claim 2 wherein said first amino acid sequence is SEQ ID NO:11.
- 14. The polynucleotide according to claim 2 wherein said second amino acid sequence is SEQ ID NO:32.
- 15. The polynucleotide according to claim 2 wherein said first amino acid sequence is SEQ ID NO:43.
- 16. The polynucleotide according to claim 2 wherein said second amino acid sequence is SEQ ID NO:41.
- 17. The polynucleotide of claim 1 wherein said polynucleotide comprises a first segment and a second segment, wherein said first segment encodes said first amino acid sequence and said second segment encodes said second amino acid sequence, and wherein said second segment is 5′ to said first segment.
- 18. The polynucleotide of claim 1 wherein said second amino acid sequence is at the carboxy terminus of said protein and said first amino acid sequence is at the amino terminus of said protein.
- 19. A transgenic host cell comprising a polynucleotide of claim 1, wherein said cell is selected from the group consisting of a plant cell and a bacterial cell.
- 20. The polynucleotide according to claim 2, wherein said first amino acid sequence is SEQ ID NO:11.
- 21. The polynucleotide according to claim 2 wherein said second amino acid sequence is SEQ ID NO:32.
- 22. The polynucleotide of claim 2 wherein said polynucleotide comprises a first segment and a second segment, wherein said first segment encodes said first amino aid sequence and said second segment encodes said second amino acid sequence, and wherein said second segment is 5′ to said first segment.
- 23. The polynucleotide of claim 2 wherein said second amino acid sequence is at the carboxy terminus of said protein and said first amino acid sequence is at the amino terminus of said protein.
- 24. A transgenic host cell comprising a polynucleotide of claim 2, wherein said cell is selected from the group consisting of a plant cell and a bacterial cell.
CROSS-REFERENCE TO A RELATED APPLICATION
This application is a continuation-in-part of U.S. Ser. No. 09/378,088, filed Aug. 20, 1999 now U.S. Pat. No. 6,372,480, which is a continuation-in-part of Ser. No. 08/844,188, filed Apr. 18, 1997 now U.S. Pat. No. 6,127,180, which is a continuation-in-part of Ser. No. 08/633,993, filed Apr. 19, 1996, which issued as U.S. Pat. No. 6,083,499 on Jul. 4, 2000.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
5723758 |
Payne et al. |
Mar 1998 |
A |
6172281 |
Van Mellaert et al. |
Jan 2001 |
B1 |
Foreign Referenced Citations (1)
Number |
Date |
Country |
WO 0024904 |
May 2000 |
WO |
Non-Patent Literature Citations (1)
Entry |
Hofte et al., “Insecticidal Crystal Proteins of Bacillus thuringiensis,” Microbiological Reviews, Jun. 1989, p. 242-255, vol. 53, No. 2. |
Continuation in Parts (3)
|
Number |
Date |
Country |
Parent |
09/378088 |
Aug 1999 |
US |
Child |
09/643596 |
|
US |
Parent |
08/844188 |
Apr 1997 |
US |
Child |
09/378088 |
|
US |
Parent |
08/633993 |
Apr 1996 |
US |
Child |
08/844188 |
|
US |