Claims
- 1. A composition comprising a photoactivatable nucleic acid derivative comprising a nucleic acid having one or more photoreactive groups bound thereto, wherein the photoreactive groups each generate an active species selected from the group consisting of nitrenes, carbenes, and excited states of ketones upon absorption of electromagnetic energy.
- 2. A composition according to claim 1 wherein the photogroup(s) comprise photoactivatable aryl ketones.
- 3. A composition according to claim 2 wherein the aryl ketones are selected from the group consisting of acetophenone, benzophenone, anthraquinone, anthrone, and derivatives thereof.
- 4. A composition according to claim 1 wherein the photoreactive group(s) are directly and covalently bound to the nucleic acid.
- 5. A composition according to claim 4 wherein at least one photoreactive group is bound to either the 3′-or 5′-ends of the nucleic acid.
- 6. A composition according to claim 5 wherein the terminal photoreactive group comprises a photoactivatable aryl ketone.
- 7. A composition according to claim 6 wherein the aryl ketone is selected from the group consisting of acetophenone, benzophenone, anthraquinone, anthrone, and derivatives thereof.
- 8. A composition according to claim 1 wherein the photoreactive group(s) are indirectly and covalently bound to the nucleic acid.
- 9. A composition according to claim 8 wherein the nucleic acid and photoreactive group(s) are bound to a common structure selected from the group consisting of synthetic and natural polymers.
- 10. A composition according to claim 9 wherein at least one photoreactive group is indirectly bound to either the 3′- or 5′- ends of the nucleic acid.
- 11. A composition according to claim 10 wherein the terminal photoreactive group comprises a photoactivatable aryl ketone.
- 12. A composition according to claim 11 wherein the aryl ketone is selected from the group consisting of acetophenone, benzophenone, anthraquinone, anthrone, and derivatives thereof.
- 13. A composition according to claim 1 wherein the nucleic acid is a synthetic oligonucleotide.
- 14. A composition according to claim 13 wherein the oligonucleotide has been synthesized by means of a phosphoramidite synthetic method.
- 15. A method of preparing a composition comprising a photoactivatable nucleic acid derivative, the method comprising the steps of derivatizing a nucleic acid with one or more photoreactive groups, wherein the photoreactive groups each generate an active species selected from the group consisting of nitrenes, carbenes, and excited states of ketones upon absorption of electromagnetic energy.
- 16. A method according to claim 15 wherein the nucleic acid is derivatized by either enzymatic modification or chemical modification.
- 17. A method according to claim 16, wherein the nucleic acid is derivatized by a chemical modification that comprises the steps of incorporating a thermochemically reactive group into a synthetic oligonucleotide, and reacting the resultant oligonucleotide with a photoreactive compound that comprises a corresponding reactive group and a photoreactive group.
- 18. A method according to claim 17 wherein the reaction between the thermochemically reactive group and corresponding reactive group is selected from the group consisting of reactions between an amine group with an N-oxysuccinimide ester, a carboxylic acid chloride with an amine, and a maleimide group with a sulfhydryl group.
- 19. A method according to claim 17 wherein the chemical modification step comprises the incorporation of one or more photoreactive groups into the nucleic acid by means of a heterobifunctional reagent comprising a thermochemically reactive group and a photoreactive group.
- 20. A method of preparing a photoactivatable nucleic acid derivative, the method comprising the steps of covalently attaching one or more photoreactive groups to a synthetic oligonucleotide in the course of oligonucleotide synthesis, wherein the photoreactive groups each generate an active species selected from the group consisting of nitrenes, carbenes, and excited states of ketones upon absorption of electromagnetic energy
- 21. A method according to claim 20 wherein the method comprises the steps of a) providing one or more nucleotide building blocks derivatized with one or more photoreactive groups, and b) using the derivatized nucleotide in the synthesis of an oligonucleotide in a manner that permits the photoreactive group to become attached to the oligonucleotide at one or more designated points.
- 22. A method of treating a surface comprising the step of providing a composition according to claim 1 and activating the photoreactive groups under conditions suitable to attach the nucleic acid to the surface.
- 23. A surface bearing an immobilized nucleic acid, the surface having been prepared by the method of claim 22.
- 24. A probe array comprising a plurality of nucleic acids covalently attached thereto, wherein the nucleic acids are covalently attached via the residues of activated photoreactive groups.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 08/916,913 for “Latent Reactive Polymers with Biologically Active Moieties”, filed Aug. 15, 1997.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
08916913 |
Aug 1997 |
US |
Child |
09028806 |
Feb 1998 |
US |