Claims
- 1. Apparatus for transferring a liquid reagent from a reagent container to a reaction vessel comprising:
- (a) a pipette having a storage volume at least equal the volume to be taken from said reagent container;
- (b) pump means including a piston and a cylinder;
- (c) a flexible conduit filled with air coupling said pipette to said pump means;
- (d) means for positioning said pipette vertically and rotationally in a horizontal plane to locate said pipette above said reagent container or in either said reagent container or said reaction vessel;
- (e) means for absorbing liquid disposed over said reagent container in the path of travel of the pipette to and from said reagent container said aborbing means being an irrigated sponge which is penetrable by said pipette in both directions travelled thereby to wipe the outer portions thereof; and
- (f) means for controlling said pump means to cause said pipette to draw in reagent in excess of the required reagent volume to be deposited in the reaction vessel, to cause said pipette to discharge only the required amount of reagent into said reaction vessel, and then to cause said pipette to expel the excess volume onto said absorbing means when located above said reagent container.
- 2. Apparatus as in claim 1 wherein said pipette positioning means comprise:
- (a) a central shaft mounted for vertical axial translation and rotational movement;
- (b) a cantilever arm attached to one end of said shaft, the pipette being attached to the free end of the arm; and
- (c) means to rotate and axially displace said shaft.
- 3. Apparatus as in claim 2 wherein said means to rotate and axially displace said central shaft comprise:
- (a) a butterfly cam member having channels on its underside coupled to said central shaft;
- (b) a motor drive means having a drive shaft; and
- (c) an arm having one end coupled to said drive shaft and terminating at its other end in a ball resting in said channels, rotation of said arm causing said butterfly cam and the central shaft therewith to first be pushed upward and then rotated and then lowered in response to rotation of said drive shaft.
- 4. Apparatus as in claim 3 wherein said butterfly cam contains a further cam follower surface and said apparatus further includes a cam on said drive shaft arranged to contact the follower surface on said butterfly cam at a point of rotation where the ball on said arm looses contact with said butterfly cam, further rotation of said drive shaft causing a further lowering of said butterfly cam and said central shaft whereby the final lowering of said pipette is accurately controlled.
BACKGROUND OF THE INVENTION
This application is a continuation of Ser. No. 499,792 filed on Aug. 22, 1974, and now abandoned.
US Referenced Citations (12)
Continuations (1)
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Number |
Date |
Country |
Parent |
499792 |
Aug 1974 |
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