Claims
- 1. A DNA construct comprising: a first expression cassette, a second expression cassette, and a third expression cassette, wherein said first expression cassette in operable linkage comprises (i) a Figwort mosaic virus 35S promoter; (ii) a heterologous 5′ untranslated leader; (iii) a chloroplast transit peptide DNA sequence; (iv) a DNA sequence encoding a glyphosate tolerant EPSPS; and (v) a transcriptional terminator; and said second expression cassette comprising in operable linkage (a) an Arabidopsis Elongation factor 1α promoter with homologous EF1α intron; (b) a chloroplast transit peptide DNA sequence; (d) a DNA sequence encoding a glyphosate tolerant EPSPS; and (e) a transcriptional terminator; and said third expression cassette comprising in operable linkage (I) an Arabidopsis Act2 promoter with homologous Act2 intron; (II) a chloroplast transit peptide DNA sequence; (III) a DNA sequence encoding a glyphosate tolerant EPSPS; and (IV) a transcriptional terminator.
- 2. A plant comprising the DNA construct of claim 1.
- 3. A plant of claim 2, wherein said plant is tolerant to glyphosate.
- 4. A plant of claim 3, wherein said plant is a dicot plant.
- 5. A method of controlling weeds, the method comprising: (1) providing a transformed crop plant with a DNA construct of claim 1; (2) selecting said transformed crop plant for tolerance to glyphosate herbicide; and (3) applying to the field in which the transformed crop plant is being grown a sufficient amount of glyphosate to inhibit weed growth without affecting the growth of said transformed crop plant.
CONTINUING DATA
This application claims priority to provisional application 60/171,173, filed Dec. 16, 1999.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5633435 |
Barry et al. |
May 1997 |
A |
Provisional Applications (1)
|
Number |
Date |
Country |
|
60/171173 |
Dec 1999 |
US |