Claims
- 1. A polynucleotide optimized for expression in a plant, wherein said polynucleotide comprises a sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:22, and SEQ ID NO:24.
- 2. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:7.
- 3. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:8.
- 4. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:9.
- 5. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:10.
- 6. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:12.
- 7. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:13.
- 8. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:16.
- 9. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:17.
- 10. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:18.
- 11. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:19.
- 12. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:20.
- 13. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:22.
- 14. The polynucleotide sequence according to claim 1 wherein said sequence is SEQ ID NO:24.
- 15. A cell comprising a plant-optimized polynucleotide selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:22, and SEQ ID NO:24; wherein said cell is selected from the group consisting of a plant cell and a microbial cell.
- 16. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:7.
- 17. The cell according to claim 16 wherein said plant cell is a cotton plant cell.
- 18. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:8.
- 19. The cell according to claim 18 wherein said plant cell is a maize plant cell.
- 20. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:9.
- 21. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:10.
- 22. The cell according to claim 21 wherein said plant cell is a cotton plant cell.
- 23. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:12.
- 24. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:13.
- 25. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:16.
- 26. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:17.
- 27. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:18.
- 28. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:19.
- 29. The cell according to claim 28 wherein said plant cell is a dicot plant cell.
- 30. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:20.
- 31. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:22.
- 32. The cell according to claim 15 wherein said cell is a plant cell and said polynucleotide is SEQ ID NO:24.
- 33. A DNA construct that comprises a polynucleotide and a promoter region active in a plant cell, wherein said promoter region is operatively linked to said polynucleotide and said polynucleotide is under the control of said promoter region, and wherein said polynucleotide comprises a sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:22, and SEQ ID NO:24.
- 34. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:7.
- 35. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:8.
- 36. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:9.
- 37. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:10.
- 38. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:12.
- 39. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:13.
- 40. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:16.
- 41. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:17.
- 42. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:18.
- 43. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:19.
- 44. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:20.
- 45. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:22.
- 46. The DNA construct according to claim 33 wherein said sequence is SEQ ID NO:24.
- 47. A method of producing transgenic plant cells wherein said method comprises transforming a plant cell with a polynucleotide comprising a sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:22, and SEQ ID NO:24; and culturing said plant cell under conditions whereby descendent generations of transgenic plant cells are produced.
- 48. The method according to claim 47 wherein said sequence is SEQ ID NO:7.
- 49. The method according to claim 47 wherein said sequence is SEQ ID NO:8.
- 50. The method according to claim 47 wherein said sequence is SEQ ID NO:9.
- 51. The method according to claim 47 wherein said sequence is SEQ ID NO:10.
- 52. The method according to claim 47 wherein said sequence is SEQ ID NO:12.
- 53. The method according to claim 47 wherein said sequence is SEQ ID NO:13.
- 54. The method according to claim 47 wherein said sequence is SEQ ID NO:16.
- 55. The method according to claim 47 wherein said sequence is SEQ ID NO:17.
- 56. The method according to claim 47 wherein said sequence is SEQ ID NO:18.
- 57. The method according to claim 47 wherein said sequence is SEQ ID NO:19.
- 58. The method according to claim 47 wherein said sequence is SEQ ID NO:20.
- 59. The method according to claim 47 wherein said sequence is SEQ ID NO:22.
- 60. The method according to claim 47 wherein said sequence is SEQ ID NO:24.
- 61. A pesticidal toxin comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:11, SEQ ID NO:15, SEQ ID NO:21, SEQ ID NO:25, and SEQ ID NO:27.
- 62. A method for controlling a plant pest, wherein said method comprises contacting said pest with a toxin produced by a transgenic plant, wherein said toxin is present in said plant, wherein said plant comprises a polynucleotide sequence optimized for expression in said plant, and wherein said polynucleotide comprises a sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:22, and SEQ ID NO:24.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The subject application is a continuation of U.S. Ser. No. 09/178,252 (filed Oct. 23, 1998), which claims priority to U.S. Provisional Patent Application Ser. No. 60/065,215 (filed Nov. 12, 1997) and to U.S. Provisional Patent Application Ser. No. 60/076,445 (filed Mar. 2, 1998).
Provisional Applications (2)
|
Number |
Date |
Country |
|
60065215 |
Nov 1997 |
US |
|
60076445 |
Mar 1998 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09178252 |
Oct 1998 |
US |
Child |
09826660 |
Apr 2001 |
US |