Plasmid DNA from Yersinia pestis

Abstract

The complete DNA sequence of three plasmids from the bacterium Yersinia pestis, the causative agent for bubonic plague, have been determined and are set forth. The open reading frames, or protein coding regions, of the plasmids have been determined. The DNA sequence and ORF information is useful for the creation of diagnostic, prophylactic and therapeutic tools for combating the disease caused by this agent.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

Not applicable.

BACKGROUND OF THE INVENTION

Over the centuries, the bubonic plague (also known as the Black Death) has claimed the lives of millions of people. The disease is characterized by chills, fever, vomiting, diarrhea, painful swollen lymph nodes (buboes), blackening of the skin caused by ruptured blood vessels, and a very high mortality rate (up to 75% if left untreated). Treatment with antibiotics in the early stages of the infection is generally effective.

Bubonic plague is caused by the bacterium Yersinia pestis , which is transmitted to humans from rats or other rodents by fleas that feed on infected rodents and then bite humans. Reservoirs of the bacteria persist today, and attempts to eliminate wild rodent plague have proven ineffective. Occasional outbreaks of the deadly disease continue to occur, particularly in small towns, villages, and rural areas in developing countries.

While bacteria carry genetic material in their chromosomes, bacteria also often carry genetic material in loops of DNA called plasmids. Bacterial plasmids are nonessential, extrachromosomal genetic elements capable of autonomous replication. The genetic material in plasmids often encodes functions required for maintenance of the plasmid in its bacterial host and sometimes encodes optional functions that promote survival of the bacterial host under certain environmental conditions. Pathogenicity determinants are commonly plasmid-encoded, and fall within the category of optional plasmid-encoded functions.

Yersinia pestis is a facultative intracellular parasite which harbors at least three different plasmids, designated pCD1, pPCP1, and pMT1, which are necessary for full virulence of the organism. One of the plasmids, designated pCD1, is also found in the enteropathogenic species Yersinia pseudotuberculosis and Yersinia enterocolitica (Ferber, et al. Infect. Immun . 31:839-841, 1981; Portnoy, et al. Curr. Topics Microbiol. Immunol . 118:29-51, 1985), whereas pMT1 and pPCP1 are unique to Y. pestis (Brubaker. Clinical Microbiol Rev . 4:309-324, 1991). Plasmids pMT1 and pPCP1 are thought to promote deep tissue penetration by Y. pestis and to contribute to the acute infection associated with this species. The Y. pestis genome shares much homology with that of Y. pseudotuberculosis , (Bercovier, et al. Curr. Microbiol . 4:225-229, 1980; Moore, et al. Inter. J. Sys. Bacteriol . 25:336-339, 1975), yet the infection caused by the latter organism is usually mild and self limiting (Butler, Plague and other yersinia infections, p. 111-159. In W. B. Greenbugh III and T. C. Merigan (eds.), Current topics in infectious disease, Plenum Press, New York, N.Y., 1983).

An understanding of the differences in the pathogenesis of Y. pestis and Y. pseudotuberculosis may be afforded by comparing polynucleotide sequences or genes found on pMT1 or pPCP1 plasmids, and which are unique to Y. pestis . It has been found that Y. pestis strains lacking the pCD1 plasmid are completely avirulent. Therefore, determination of the complete pCD1 sequence may provide important information about the role of the plasmid in virulence in various pathogenic yersiniae.

The 9.5 kb plasmid pPCP1 encodes a bacteriocin termed pesticin, a pesticin immunity protein and a plasminogen activator activity. Loss of this plasmid increases the LD 50 of the organism by a factor of one hundred thousand, as measured by subcutaneous injection in the mouse model. (Sodeinde, et al. Science 258:1004-1007, 1992).

The second plasmid unique to Yersinia pestis , designated pMT1, is a 100 kb plasmid that encodes the capsular protein Fraction 1 and the murine toxin (Protsenko, et al. Genetika 19:1081-1090, 1983). The genes for the capsular proteins have been cloned and sequenced using Y. pestis strain EV76 (Galyov, et al. FEBS Lett . 277:230-232, 1990; Galyov, et al. 286:79-82, 199.1; Karlyshev et al. FEBS Lett . 305:37-40, 1992). The role of these proteins in plague pathogenesis has not been unequivocally determined, and the effect of mutational loss of these proteins on the LD 50 varies, depending on the animal model and route of infection (Brubaker Curr. Top. Microbiol . 57:111-118, 1972; Brubaker Rev. Infect. Diis. 5: S748-S758, 1983). However, pMT1 does appear to contribute to the acute phase of plague infection, as evidenced by a reduced morbidity associated with infection by strains lacking pMT1 (Drozdov, et al. J. Med. Microbiol . 42:264-268, 1995; Samoilova, et al. J. Med. Microbiol . 45:44.0-444, 1996;Welkos, et al. Contrib. Microbiol. Immunol . 13:229-305, 1995).

Information pertaining to the genetic characterization of the pMT1 molecule is limited. The size of the plasmid has been found to vary, either from variations in the versions of the plasmids or in technique to measure the plasmids, from 90 kb to 288 kb (Filippov, et al. FEMS Microbiol. Lett . 67:45-48, 1990). It is known that pMT1 is an integrative plasmid capable of integrating into Y. pestis chromosome with high frequency and at multiple sites, with integration likely resulting from IS100 homology between the plasmid and chromosome (Protsenko, et al. Microbiol. Pathogen 11:123-128, 1991).

Previous characterization of pMT1 has identified five genes that may be involved in the synthesis of murine toxin (MT) and F1 capsule antigen, both known virulence factors. Expression of both the capsular protein and murine toxin genes has been characterized with respect to environmental cues (e.g., temperature and-calcium) (Du, et al. Contrib. Microbial. Immunol . 13:321-324, 1995). F1 capsule synthesis is maximal at 37° C. in the absence of extracellular calcium, conditions similar to those that induce expression of a major Y. pestis virulence determinant (Straley Rev. Infect. Dis . 10:S323-S326, 1988; Straley Microbial. Pathoaen 10:87-89, 1991; Straley et al. Proc. Natl. Acad. Sci. USA 78:1224-1228, 1981). Murine toxin expression is induced at 26° C., conditions similar to those that would be expected to occur in the flea vector. The occurrence of plasmid genes that are induced under widely different conditions suggests regulation of Y. pestis virulence determinant expression by at least two networks.

The plasmid pCD1 is found in Y. pestis , as well as in certain other pathogenic Yersinia species, including Y. pseudotuberculosis and Y. enterocolitica . The plasmid encodes a complex virulence property called the low-Ca 2+ response (LCR). The LCR was discovered in Y. pestis growing in vitro, where the bacteria respond to the absence of Ca 2+ at 37° C. by the strong expression and secretion of a virulence protein called V antigen, or LcrV. In certain media, expression of LcrV is accompanied by a response termed “restriction,” in which the yersiniae undergo an orderly metabolic shutdown and cease growth. Under LCR-inductive conditions, the transcription, translation, and secretion of a set of virulence proteins called Yops (for Y ersinia o uter p rotein s ) is maximally induced. The operons encoding these and other similarly regulated operons on the LCR plasmid have been referred to as the LCR stimulon (LCRS). Millimolar concentrations of Ca 2+ permit full growth at 37° C., reduced expression of LcrV and Yops, and essentially no secretion of these proteins. Under ambient temperature conditions outside a mammalian host, the Yops and LcrV proteins are produced at a low, basal level and are not secreted, which suggests that the LCR is designed to function within a mammal. Expression of LCR is apparently modulated by other environmental factors, including Mg 2+ , Cl − , Na + , glutamate, nucleotides, and anaerobiosity. The molecular basis for these effects has not been determined, but these elements of environmental modulation could be important in adjusting virulence protein expression and secretion in response to the wide range of niches that yersiniae are expected to encounter during an infection.

The pCD1 plasmid also encodes a type III secretion system called Ysc (for Y op s e c retion) that is involved in the secretion of Yops, LcrV, and some regulatory proteins in the LCR. The Ysc system is locally activated by cell contact at the interface between a bacterium and eukaryotic cell. This cell to cell contact causes the opening of the secretion system's inner and outer gates (LcrG and LcrE (or YopN), respectively), thereby allowing secretion of negative regulatory proteins (e.g., LcrQ also called YscM, a key regulatory protein). Secretion of negative regulatory proteins allows full transcriptional activation of LCRS operons by LcrF, an AraC-like activator protein.

Yops are secreted locally, without processing. The secretion mechanism recognizes two signals: one in the first 45 nucleotides of the yop mRNA and one related to a domain that has been found for some Yops to bind a specific Yop chaperone (Syc), also encoded by the LCR plasmid Certain of the Yops (e.g., YopB, YopD, YopK) are involved in targeting effector Yops (YopE, YopH, YpkA, YopM, and possibly YopJ) into the eukaryotic cell. Once inside the cell, the effector Yops act on intracellular target molecules, thereby interfering with cellular signaling and cytoskeletal functions. LcrV acts functions both as a regulatory protein involved in Yop secretion and targeting and as a potent anti-host protein. LcrV is the only LCRS protein that is secreted in large amounts into the surrounding medium by yersiniae in contact with eukaryotic cells. LcrV adversely affects the host organism when administered alone to mice, whereas all other secreted proteins depend on the Ysc machinery of yersiniae, in intimate contact with mammalian cells, for delivery into the mammalian cells.

Expression of the LCR has a profound immunosuppressive effect that results from the interference with innate defenses at the site of infection and the host organism's inability to mobilize an effective cell-mediated immune response. Y. pestis , and, in immunocompromised individuals, the enteropathogenic yersiniae grow unchecked in the lymphoid system in a fulminant-disease associated with high mortality, absent appropriate antibiotic treatment. In contrast, yersiniae lacking the LCR plasmid pCD1 are completely avirulent.

Several other important pathogens have virulence systems with many striking similarities to the LCR; however, the LCR is the best characterized of these and remains a prototype for investigations at the forefront of molecular pathogenesis.

A more complete understanding of the role of LCR plasmids may be obtained by determining the entire sequence of an LCR plasmid.

The development of additional sequence information from plasmids of Y. pestis is needed for comprehensive efforts in the detection, diagnosis, prophylaxis and treatment of infections caused by the organism.

BRIEF SUMMARY OF THE INVENTION

One aspect of the present invention is an isolated Yersinia pestis plasmid pMT1- or pCD1-specific polynucleotide sequence selected from the group consisting of any portions of the sequences present in SEQ ID NO:1 through SEQ ID NO:6 set forth below.

The present invention is in part summarized by the presentation of the complete nucleotide sequence of two plasmids from Yersinis pestis , which enables diagnostic, prophylactic and therapeutic tools to be developed for use in combating the pathogen.

The DNA sequences of the present invention may include an open reading frame (ORF), an insertion sequence element, or a plasmid maintenance function, for example.

It is an object of the invention to provide essentially the entire sequence of pMT1 and pCD1 from Yersinia pestis KIM5 to allow methods of detecting, diagnosing, preventing, and treating infections with Yersinia pestis.

Other object, advantages and features of the present invention will become apparent from the following specification when taken in conjunction with the following drawings.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS

FIG. 1 is a plasmid map of the plasmid pMT1, showing in schematic fashion the relative positions of notable features of the plasmid.

FIG. 2 is a similar plasmid map of the plasmid pCD1.

DETAILED DESCRIPTION OF THE INVENTION

This specification describes the complete DNA sequencing of the plasmids , pPCP1, pMT1 and pCD1 from Yersinia pestis , all of which are associated with the pathogenicity of the organism. Presented below is both the complete DNA sequence of the plasmids as well as tables listing the open reading frames (ORFs) of the plasmids, indicating which portions of the plasmid DNA encodes the production of proteins. Some other important regions of the plasmid DNA, such-as the integration sequences (IS) are also indicated. With the information provided by this complete DNA sequence information, several things become possible. It now becomes possible to design and implement nucleotide-sequence based diagnostic tools to diagnose and identify virulent strains of Yersinia pestis in a biological sample based on the presence of DNA sequence in such a sample. The identification of the ORFs contained in the plasmids makes possible the comprehensive identification and characterization of the toxins and other proteins encoded by the plasmids thereby enabling the ability to make antibody and other molecular forms of prophylactic and therapeutic treatment for the pathogen. This information also allows identification of new potential virulence factors that may be useful in the development of vaccines, or which may be suitable targets for therapeutic drugs. In addition, the sequencing data provides information about maintenance functions, horizontal gene transfer, conjugation, integration, insertion sequence (IS) elements, and evolution of these plasmids. The sequences from pCD1 and pMT1, and their significance, were first published by the inventors here in Lindler et al. Inf. Immunity 66:5731-5742, 1998 and Perry et al. Inf. Immunity 66:4611-4623, 1998, both of which are incorporated herein by reference in their entirety. Identification of maintenance functions provides information that is useful in designing cloning vectors, which can be used, for example, to study factors associated with pathogenicity.

Briefly, as described below in the examples, we determined the entire nucleotide sequence of the plasmid pMT1 from Y. pestis strain KIM5. We then analyzed the sequence and identified potential open reading frames (ORFs) encoded by the 100,990 bp pMT1 molecule. The complete sequence is set contained in SEQ ID NO 2 below. Based on yersinial codon usage for known yersinial genes, homology with known proteins in the databases and potential ribosome binding sites, it was determined that 115 of the potential ORFs likely encode proteins in Y. pestis . Seven new potential virulence factors that might interact with the mammalian host or flea vector were identified. The deduced amino acid sequences for 43 of the remaining 115 putative ORFs display no significant homology to proteins in the current databases. Furthermore, DNA sequence analysis allowed the determination of the putative replication and partitioning regions of pMT1.

A single 2,450 bp region within pMT1 that may function as the origin of replication (ori) was identified. The identification of this putative ori may allow construction of cloning vectors capable of replicating in Yersinia species. Such vectors will facilitate further research into the pathogenicity of these bacteria. The putative ori includes a RepA-like protein similar to those of the RepFIB, RepHI1B, P1 and P7 replicons. A plasmid partitioning function is located about 36 kilobases from the putative origin of replication and is most similar to the parABS bacteriophage P1 and P7 system. Y. pestis pMT1 encodes potential genes with a high degree of similarity to a wide variety of organisms, plasmids and bacteriophage. Accordingly, our analysis of pMT1 DNA sequence suggests the mosaic nature of this large bacterial virulence plasmid and provides insight into its evolution. The MT- and F1 encoding regions of pMT1 are surrounded by remnants of multiple transposition events and bacteriophage, respectively, suggesting horizontal gene transfer of these virulence factors.

The pCD1 sequence is 70,509 base pairs, and is presented as SEQ ID NO:1 herein. The SEQ ID NO:1 is actually 70,559 base pairs in length since it incorporates a 50 base pair repeat at each end of the linear representation of the circular plasmid. Sequencing of pCD1 has revealed a potential new Yop and Yop chaperone, two new IS, a set of LCRS genes very similar to those sequenced in the enteropathogenic yersinae, the IncFIIA replication region, and SopABC partitioning functions. Remnants of IS elements were found to be scattered throughout the plasmid, which suggests that pCD1 has undergone numerous insertional events as well as genetic recombinations and rearrangements during its history.

Yersinia pestis has an unique 9.5 kb plasmid, designated pPCP1, which contains genes encoding plasminogen activator/coagulase and pesticin. The total length of pPCP1 is 9,610 bp with a GC of 43%. The plasmid pPCP1 contains a copy of IS100. Three known gene functions located on this plasmid are as follows: 1) plasminogen activator and coagulase activity that is encoded on the same gene (pla), 2) pesticin, a toxin that inhibits growth of closely related bacteria, and 3) pesticin immunity gene whose product protects the bacteria from toxic effects of the pesticin. The origin of replication of pPCP1 is encoded on 780 bp region which is very similar to the origin of replication and the immunity region of Escherichia coli ColE1 plasmid. Loss of this plasmid leads to ineffective infection in guinea pigs and mice suggesting that the plasmid plays an important role in the invasion and infection of its mammalian host. The plasmid pPCP1 has also been sequenced and its sequence is presented as SEQ ID NO:3.

The sequences presented here are accurate to the best capabilities of the current state of the art, but may contain some minor errors, deletions, insertions or substitutions. It is also understood and expected that other strains of the host organism will have allelic variations of the genes in the host and therefore may carry different forms of the genes set forth in the sequence listing here. However, those of skill in the art expect such minor variations, and such minor sequence variations in Yersinia pestis -specific nucleotide sequences associated with nucleotide additions, deletions, and mutations, whether naturally occurring or introduced in vitro, would not interfere with the usefulness of these sequences in the detection of Yersinia pestis , in preventing Yersinia infection, and in methods for treating Yersinia pestis infection. Therefore, the scope of the present invention is intended to encompass such variations in the claimed sequences.

A Yersinia pestis -specific nucleotide probe is a sequence that is able to hybridize to Yersinia pestis target DNA present in a sample containing Yersinia pestis under suitable hybridization conditions, and which does not hybridize with DNA from other Yersinia species or from other bacterial species. It is well within the ability of one skilled in the art to determine suitable hybridization conditions based on probe length, G+C content, and the degree of stringency required for a particular application.

The probe may be RNA or DNA. Depending on the detection means employed, the probe may be unlabeled, radiolabeled, or labeled with a dye. The probe may be hybridized with a sample that has been immobilized on a solid support such as nitrocellulose or a nylon membrane, or the probe may be immobilized on a solid support, such as a silicon chip.

The sample to be tested for presence or absence of Yersinia pestis DNA may include blood, urine, feces, or other materials from a human, rodent, or flea susceptible to infection by yersinia pestis . The sample may be tested directly, or may be treated in some manner prior to testing. For example, the sample may be subjected to PCR amplification using appropriate oligonucleotide primers. To have reasonable assurance of success under conditions of variable stringency, it is preferred that such diagnostic probes uses sequences which are at least 15 nucleotides or longer in length. While probes as short as 15 base pairs can be made to work, probes of at least 25 base pairs or longer are preferred. Any means of detecting DNA-RNA or DNA-DNA hybridization known to the art may be used in the present invention. Since the plasmids set forth below are diagnostic of pathogen strains of Yersinia pestis , any set of 25-mers or longer from the sequences set forth below may usefully be employed as diagnostic probes for the presence of this pathogen in a biological sample.

Any and all of the ORFs presented here are of particular utility. Since these ORFs contain the coding regions for the proteins expressed by these plasmids, these ORFs are not just useful for diagnosis of the presence of the pathogenic host, they may be used to express the encoded proteins in other hosts. Placing the coding regions of-the ORFs under the control of non-native promoters permits the expression of the proteins encoded by the ORFs in other hosts. The ORFs can be inserted into any known expression vector adapted for a particular host and then can be transformed into that host for expression to produce proteins. Such proteins can be used for both prophylactic and therapeutic purposes. The proteins can be used to generate antibodies to the proteins natively produced by the Y. pestis , the provide pathogen specific. antibodies for diagnostic or therapeutic purposes. Proteins, or even peptides from the proteins have potential for targets for vaccination studies.

EXAMPLES

Isolation of pMT1 DNA

Y. pestis KIM10+ (Perry, et al. J. Bacteriol . 172:5929-5937, 1990), a strain that contains only pMT1, was grown in Heart Infusion Broth (Difco Laboratories, Detroit, Mich.) at 26-30° C. Plasmid DNA was isolated from the bacteria using alkaline lysis and polyethylene glycol precipitation (Birnboim, et al. Nucleic Acids Res . 7:1513-1523, 1979; Humphreys, et al. Biochim. Biophys. Acta 383:457-463, 1975). DNA libraries were prepared from purified pMT1, as described below.

Isolation of pCD1 DNA

Y. pestis strain KIM5 is conditionally avirulent due to deletion of the 102 kb pgm locus; it possesses all three prototypical Y. pestis plasmids (pPCP1, pCD1, and pMT1). Plasmid DNA was isolated from Y. pestis KIM5 by alkaline lysis followed by precipitation with polyethylene glycol. A mixture of pCD1 and pBR322 was transformed into Escherichia coli HB101. Transformants containing pBR322 were selected on the basis of ampicillin resistance. Ampicillin resistant transformants were transferred to nitrocellulose membranes and hybridized against pCD1 radioactively-labeled by nick translation, which allowed identification of cotransformants containing both pCD1 and pBR322. A selected cotransformant was cured of pBR322 by fusaric acid selection and used for isolation of pCD1. The pCD1 plasmid appears to be stably maintained in E. coli HB101. Plasmid DNA from E. coli HB101 (pCD1) cells grown in Luria broth was isolated by alkaline lysis followed by further purification with polyethylene glycol. Purified pCD1 DNA was used in subsequent sequencing.

pPCP1

DNA of pPCP1 was isolated for sequencing in a similar fashion.

DNA Sequencing.

DNA libraries of pPCP1, pCD1 or MT1 were prepared from nebulized, size fractionated plasmid DNA (Millon, et al. Gene, submitted) in the M13 Janus vector (Burland, et al. Nucleic Acids Res. 21:33.85-3390, 1995). DNA templates were purified from random library clones (Romantschuk, et al. Mol. Microbiol. 5:617-622, 1991), and DNA sequencing was preformed using dye-terminator labeled fluorescent cycle sequencing Prism reagents and ABI377 automated sequencers (Applied Biosystem Division of Perkin-Elmer). Sequences were assembled into segments of DNA sequence, referred to as contigs, by the SeqMan II program (DNASTAR), and clones were selected for sequencing from the opposite end to fill in coverage, resolve ambiguities and close gaps. Final coverage was about eight fold. The complete sequences of ball three plasmids are set forth in SEQ ID NO: 1 through 3 below.

In several instances,. pCD1 sequences differed from previously published sequences from the yersiniae or yielded unexpected results. To ensure that this did not result from mutations to pCD1 during carriage in E. coli , we sequenced these regions using pCD1 isolated from the conditionally virulent Y. pestis strain KIMS or pJIT7, a recombinant plasmid containing the IS1616 region adjacent to sopAB.

Sequence Annotation.

Open reading frames (ORFs) putatively encoding polypeptides at least 50 aa in length were identified using Geneplot or GeneQuest (DNASTAR) programs to display start codons (including GUG), stop codons and codon usage statistics plots for each reading frame. Codon usage analysis, used to predict ORFs, was assessed in the program by second and third order statistical comparisons with a matrix built from all available sequences for Yersinia species (Borodovsky, et al. Computational Chemistry 17:123-133, 1993). Although this matrix was more useful than one derived from E. coli genes, it was necessarily constructed from a relatively small data set. Generally, the start codon (including GTG and TTG) farthest upstream was used to annotate the ORF start. An ORF having fewer than 150 bases was included if it had a high codon usage score. For the first pass, putative amino acid sequences were searched against SWISS-PROT 34 using the BLOSUM26 matrix, by the DeCypher II System (TimeLogic Inc., Incline Village, Nev.).

Subsequent searches of the Swiss Protein, E. coli and non-redundant GenBank databases were obtained over the Internet using BLAST software (Altschul, et al., Nucleic Acids Res . 25:3389-3402, 1997) from the National Center for Biotechnology Information homepage (which can be found on the world wide web under ncbi.nlm.gov/BLAST). Pairwise protein alignments were with the BLAST algorithm. Protein localization was predicted for relevant translated orfs using the PSORT program (Nakai, et al. Proteins: Structure, Function, and Genetics 11:95-110, 1991). The prediction of membrane associated helices was with the TMpred program (Hoffman, et al. Biol. Chem . 347:166-172, 1993). Where appropriate, multiple protein sequences were aligned using the algorithm developed by Lipman et. al.(Proc. Natl. Acad. Sci. USA 86:4412-4415, 1989). These programs can be found as part of Pedros Molecular Biology Tools at Internet site www.iastate.edu.

Bank Accession Number

The annotated sequence for pMT1 and pCD1 were deposited in GenBank under accession numbers AF074611 and AF074612, respectively. These deposited sequences are also hereby incorporated by reference.

Sequence of pMT1

The fully-assembled pMT1 DNA sequence is a circular DNA sequence 100,990 bp in length. A map of the plasmid is set forth in FIG. 1 , which illustrated the general location of sequences of interest. The complete DNA sequence of the plasmid is presented here as SEQ ID NO:2. Screening of the entire plasmid sequence using the DNASTAR program GeneQuest revealed 145 potential open reading frames (ORFs) along the entire length of the plasmid. The putative amino acid sequence of each ORF was used to search the various databases (GenBank, Swiss Protein,GenPept and E. coli ) for proteins with potentially significant homologies. Table 1, set forth below, identified the location and other information of interest about many of the ORFs which were found to have homologies to known sequences.

TABLE 1
ORFs identified in Y. pestis pMT1 DNA sequence by classification. a
		Function or	Organism or Element	Accession
Designation	ORF Class	Comments	(Gene if known)	Number	Location (bp)
DNA	ORF1	IS100	Y. pestis IS100 (orfB)	U59875	73,885-74,661
Metabolism	ORF2	Ligase	Bacteriophage T3	X05031	74,680-75,777
	ORF12	Integrase	Vibrio cholera	U39068	82,931-84,109
	ORF16	DNA Pol III	E. coli	M19334	88,955-92,479
	ORF26	RecA	Bacteroides fragills	M63029	96,910-97,986
			(recA)
	ORF34	RepA	E. coli plasmid ColV	L01250	Complement
					717-1,781
	ORF41	exoA	Bacteriophage T4	X01804	4,968-6,053
			(gene 47)
	ORF43	exoB	Bacteriophage T4	X01804	6,271-8,199
			(gene 46)
	ORF46	IS200	IS200	U22457	9,675-10,184
	ORF60	Rep-like	Coxiella burnetti	L34077	16,197-16,895
			plasmid pQPH1
	ORF61	SpoJ-like	Streptococcus	AF000658	16,862-17,563
			pneumoniae
	ORF69	Gene 17-like	Bacteriophage T4	X52394	20.457-21,713
			(gene 17)
	ORF93	IS100	Y. pestis IS100 (orfB)	U59875	Complement
					46,449-47,231
	ORF94	IS100	Y. pestis IS100 (orfA)	U59875	Complement
					47,228-48,250
	ORF101	IS285	Y. pestis IS285 (orf2)	X78303	51,013-52,221
	ORF102	Transposase	Enterobacter	U60777	52,648-53,712
		TN4321	aerogenases TN4321 (tn
			pA)
	ORF108	Membrane	E. Coli plasmid	U09868	Complement
		Endonuclease	pKM101 (nuc)		57,629-58,117
	ORF111	Resolvase	Pseudomonas syringae	L48985	Complement
			(stbA)		60,161-60,781
	ORF113	ParA	Bacteriophage P1	X02954	61,767-63,041
			(parA)
	ORF114	ParB	Bacteriophage P1	K02380	63,038-64,009
			(parB)
	ORF123	Adenine specific	E. coli pEC156 EcoVIII	U48806	66,648-67,325
		DNA methylase	methylase
	ORF128	Antirestriction	E coli	Z34467	69,208-69,714
	ORF135	DNA Partitioning	Rhizobium meliloti	X69105,	70,730-72,739
			(Orf1, Orf2 of	U82272,
			pRmeGR4a), Shigella	AF000658
			sonnei (psiB),
			Streptoccus
			pneumoniae (spoOJ)
	ORF136	IS100	Y. pestis IS100 (orfA)	U59875	72,863-73,882
Protein	ORF28	HflC-like	Vibrio	U09005	98,281-99,111
Metabiolism			parahaemolyticus
			(hflC)
	ORF63	ABC	Archaeglobus fulgidus	AE001029	17,500-18,198
		transporter/ATP	(AF1064)
		binding
	ORF75	L12 Ribosomal	Haloferax volcanii	X58924	25,927-26,361
		protein L12e
Gene	ORF5	Caf1R	Y. pestis (caf1R)	X61996	Complement
Regulation					77,118-78,041
	ORF22	PprB-like	Pseudomonas putida	X80272	94,557-95,636
			(pprB)
	ORF56	Repressor of	Salmonella abony (fljA)	D26167	Complement
		flagella			13,278-13,841
		synthesis
Known	ORF6	Caf1M	Y. pestis (caf1M)	X61996	78,318-79,127
Virulence					(GTG Start)
	ORF8	Caf1A	Y. pestis (caf1A)	X61996	79,152-81,653
	ORF9	Caf1	Y. pestis (caf1l)	X61996	81,734-82,246
	ORF107	Murine toxin	Y. pestis (ymt)	X92727	Complement
					55,788-57,551
Lambda-like	ORF80a	V major tail fiber	Bacteriophage lambda	P03733	28,560-29,303
		Intimin	E. coli O157:H7 (eae)	P43261
	ORF84	H tail fiber	Bacteriophage lambda	AF007380	30,041-34,618
		protein
	ORF85	M minor tail fiber	Bacteriophage lambda	P03737	34,660-34,995
		protein
	ORF86	L minor tail flber	Bacteriophage lambda	P03738	35,052-35,783
		protein
	ORF87a	K tail assembly	Bacteriophage lambda	P03729	35,815-36,570
		protein
	ORF88	I tail assembly	Bacteriophage lambda	P03730	36,561-37,148
		protein			(GTG Start)
	ORF89	J host specificity	Bacteriophage lambda	P03749	37,164-41,801
		protein
	ORF91	Hypothetical	Bacteriophage lambda	P03745	42,469-45,405
		protein ORF314
	ORF92	Tail fiber	Bacteriophage lambda	225931	45,707-46,315
		assembly	(tfa)
Hypothetical	ORF15	CobT	Pseudomonas	P29934	85,075-87,441
in database b			denitrificans (cobT)
	ORF15a	CobS	Pseudomonas	P29933	87,539-88,771
			denitrificans (cobS)
	ORF29	Hypothetical	Bacteriophage P22	X78401	99,265-99,636
		protein	(ninX)
	ORF33a	Hypothetical	Bacteriophage P1	76816	100,922-147
		regulatory
		protein
	ORF38	Hypothetical	Bacillus subtllis (orfK,	L16808,	Complement
		lipoprotein	yzeA)	Z93102	3,530-4,552
	ORF59	Long	Pyrococcus horikoshii	AB009472	Complement
		hypothetical	(PHBW005)		14,573-16,132
		protein
	ORF73	SRP1	Synechococcus	Q55032	24,271-25,146
		Hypothetical	PCC7942 pANL
		protein
	ORF104	Hypothetical	E. coli	U70214	Complement
		protein			54,405-54,803
	ORF105	Hypothetical	E. coli	U70214	Complement
		protein			54,694-55,002
	ORF116	Hypothetical	Sphingomonas S88	U51197	64,388-65,785
		protein	(spsJ)
	ORF131	Hypothetical	E. coli	AE000133	70,427-70,657
		protein
Fragments c	ORF23	DNA	Lactococcus lactis	U78771	95,646-96,641
		polymerase 1
	ORF33	Type II	Helicobacter pylori	AE000647	100,590-100,925
		restriction
		enzyme
	ORF99	Hypothetical	Methanobacterium	AE000913	Complement
		protein	thermoautotrophicum		49,210-50,004
	ORF103	Hypothetical	Salmonella	Z29513	Complement
		transposase	typhimurium		53,911-54,234
	ORF103a	IS600	Shigella sonnei	X05952	54,281-54,481
	ORF106	Hypothetical	Shigella flexneri	U97489	55,073-55,543
	ORF106a	IS801	Pseudomonas syringae	X57269	55,589-55,729
	ORF110	Hypothetical	Salmonella	Z29513	Complement
			typhimurium		59,154-60,140
	ORF115a	SamB-like	Salmonella	D90202	87,539-88,771
			typhimurium

In the above Table 1, the location of each of the ORFs is given in base pair number corresponding to the entire 100,990 base pairs of the entire plasmid. ORFs listed were assigned a putative function according to our criteria outlined in the general overview section of the results and discussion. Classification then was based on these putative functions.

If there was insufficient homology, by our criteria, with known proteins in the database the ORF has not been assigned a function in the table. In evaluating the significance of potential matches, several factors were considered. In general, if the putative translation product of a pMT1 ORF exhibits significant similarity to known proteins in the database, the putative protein was assigned a similar function. Homologies were considered to be significant if at least 25 percent of amino acids were identical over at least 35 percent of the protein in the database. The 25% identity was chosen to give a reasonable baseline, with adjustments being made for conservative amino acid substitutions to give higher similarity scores between protein molecules.

In specific instances, we have designated a protein function as “similar” based on less than 25 percent identity. The extent of homology with the database protein was set at 35 percent to allow for the possibility that protein domains might have different functions in different molecular contexts. The stringency was lowered when deciding if a putative protein might function in pathogenesis. In these cases, if the region of homology included at least 20 percent identical amino acids with a protein that might interact with or substitute for the action of a host protein, it was considered a potential virulence factor. Greater weight was given to potential alignments if the homology between the Y. pestis ORF and the target protein sequence was in a domain having a known function in host physiology. Finally, if the putative protein does not contain significant similarity to any known proteins, the upstream DNA was analyzed for ribosome binding sites (RBS) and the known codon usage for Yersinia genes was considered. After applying these criteria to the 145 potential ORFs initially identified on pMT1, 30 were eliminated and 115 putative coding regions remained. Of these 115 putative ORFs, 38 percent had no significant regions of homology to any protein in the current databases and seven percent had significant homology with previously described hypothetical proteins.

Newly Identified Virulence Factors of pMT1

Because Y. pestis is a facultative intracellular parasite and pMT1 is thought to enhance deep tissue spread of the organism, several ORFs having limited homology with proteins that may function during various stages of the plague life cycle were carefully examined. The ORFs include ORF 4 (base pairs 76,298 to 76,603), ORF 17 (bases 92,476-92,919), ORF 18 (complement to bases 92,949-93,512), ORF 21 (bases 94,015-94,448), ORF 72 (23,873-24,244), and-ORF 74a (25,221-25,883). Again, all base pairs locations refer to the complete 100,990 sequence. Additional information about these identified virulence factors is presented in Table 2, below. Although many of these homologies are below our criteria for general ORF homologies, a more relaxed standard was indicated to aid in future research relating to plague pathogenesis.

TABLE 2
ORFs that may be potential virulence factors.
ORF		Homologus	Amount of	Accession
Designation	Location	Protein (Target)	Homology a	Number	Reference
ORF4	76,298-76,603	C-type natriuretic	43/30	P41319	83
		peptide from
		Squalus
		acanthias
ORF17	92,476-92,919	Delta insecticidal	40/18	P05628	35
		protein from
		Bacillus
		thuringiensis
ORF18	Complement	RTX Toxin of	21/11	D16582	32, 65
	92,949-93,512	Actinobacillus
		pleuropneumoniae
ORF21	94,015-94,448	Laminin of Homo	23/5	Q16787	79, 95
		sapiens ,
		Paramysin-related	21/18	U20609	25, 99
		protein of
		Onchocerca
		gibsoni
ORF72	23,873-24,244	Major	24/32	P29966	41
		Myristoylated
		Alanine-rich
		Protein Kinase C
		Substrate
		(MARCKS)
ORF74a	25,221-25,883	Bacteriophage	40/41	P03733	81
		lambda V protein,
		Citrobacter	30/10	Q07591	82
		freundii intimin
a Percent identical amino acids over the percent of the total target protein sequence.

In addition, one potential new IS element, designated IS1618, is located from bp 52,465 to 53,758 (or bases 2365-3658). This sequence, the boundaries of which are defined by two directly repeated sequences (GATGATAA), flanks a putative transposase designated ORF102. ORF102 had the greatest identity with a putative transposase previously found in Enterobacter aerogenases (Smith, et al. J. Gen. Microbiol . 139:1761-1766, 1993)(40% over 96 percent of the target protein) and a putative transposase previously described in Yersinia enterocolitica (Rakin et al. FEMS Microbiol. Lett . 129:287-292, 1995) (36% identity over 96% of the target protein).

The nucleotide sequence of Y. pestis pMT1 has provided a wealth of new information. Our analysis has allowed us to identify several genes to target for further study in order to access their possible role in pathogenesis. Deciphering the potential role of these proteins improves our understanding of disease as well as host physiology. As more complete virulence plasmid DNA sequences become available, we will begin to understand the mosaic nature of these molecules and what new combinations we might expect in the future. Detailed molecular analysis of the structure of virulence plasmids will impact our ability to predict the emergence of bacterial pathogens as well as detect their presence.

Sequences of pCD1

A genetic map of the Y. pestis KIM5 pCD1 plasmid, which is 70,509 nucleotides in length, is shown in FIG. 2 . Again the complete DNA sequence of the plasmid is contained in the sequence listing appended hereto, this sequence being SEQ ID NO:1. Again the ORFs of the sequence was determined by computer analysis and searched against existing data bases. Table 3 below lists significant ORFs and their primary characteristics. Most IS element remnants and partial ORFs that appear to be nonfunctional due to IS-related events or other deletions and rearrangements are not included in Table 3.

TABLE 2
ORFs encoded on pCD1 of Y. pestis KIM5 a
			Begin-
geneb			ing	End	Number of	Isoelectric
or ORF	Function	Orientation	of ORF	of ORF	amino acids	point	kDa
repB	Negative regulator of	+	1,171	1,425	85	9.72	9.58
(copB)	repA transcription
tap	Required for translation	+	1,667	1,741	25	9.31	2.82
	of repA
repA	Plasmid replication	+	1,734	2,600	289	10.96	33.55
Orf5	Unknown	−	3,645	3,427	73	9.96	8.22
Orf7	Unknown	+	4,758	5,186	143	4.39	15.78
ypkA	Targeted effector; ser thr	+	5,204	7,402	733	6.53	81.74
(yopO)	kinase
yopJ	Targeted effector; causes	+	7,798	8,664	289	7.07	32.46
(yopP)	apoptosis in macrophages and
	interferes with cell signaling
yopH	Targeted effector; protein	+	10,347	11,753	469	8.68	50.87
	tyrosine kinase; interferes
	with cell signaling at focal
	adhesions
lcrQ	Negative regulator of LCR	−	16,148	15,801	116	6.34	12.41
(yscM)	expression
yscL	Type III secretion component	−	17,038	16,373	222	4.57	24.65
yscK	Type III secretion component	−	17,613	16,984	210	6.75	23.99
yscJ	Type III secretion component	−	18,347	17,613	245	7.43	27.04
yscI	Type III secretion component	−	18,701	18,354	116	4.47	12.67
YscH	Secreted; unknown function	−	19,199	18,702	166	5.14	18.35
(yopR)
yscG	Type III secretion component	−	19,543	19,196	116	6.60	13.07
yscF	Type III secretion component	−	19,808	19,545	88	7.13	9.49
yscE	Type III secretion component	−	20,009	19,809	67	7.31	7.61
yscD	Type III secretion component	−	21,265	20,006	420	5.85	46.93
yscC	Type III secretion component	−	23,085	21,262	608	6.49	67.35
yscB	Unknown	−	23,504	23,091	138	9.27	15.41
yscA	Unkown	−	23,828	23,730	33	9.82	3.86
lcrF	Activator or LCR expression	−	24,722	23,907	272	8.91	30.84
(virF)
yscW	YscC lipoprotein chaperone	−	25,241	24,846	132	10.12	14.71
(virG)
yscU	Type III secretion component	−	26,881	25,817	355	8.81	40.39
yscT	Type III secretion component	−	27,666	26,881	262	5.67	28.45
yscS	Type III secretion component	−	27,929	27,663	89	6.32	9.57
yscR	Type III secretion component	−	28,584	27,931	218	4.68	24.43
yscQ	Type III secretion component	−	29,504	28,581	308	5.08	34.42
yscP	Type III secretion component	−	30,868	29,501	456	5.44	50.42
yscO	Type III secretion component	−	31,332	30,868	155	7.84	19.00
yscN	Type III secretion component	−	32,648	31,329	440	6.48	47.81
lcrE	Secretion control	+	32,846	33,727	294	5.07	32.67
(yopN)
tyeA	Secretion and Yop targeting	+	33,708	33,986	93	4.21	10.75
	control
Orf42	Unknown	+	33,973	34,344	124	5.54	13.61
Orf43	Unknown	+	34,341	34,709	123	6.32	13.76
Orf44	Unknown	+	34,706	35,050	115	6.92	13.12
lcrD	Secretion	+	35,037	37,151	705	5.04	77.81
(yscV)
lcrR	Unknown	+	37,148	37,588	147	10.27	16.46
lcrG	Secretion control; efficient	+	37,630	37,917	96	8.15	11.02
	Yop targeting
lcrV	Diffusible effector; secretion	+	37,919	38,899	327	5.66	37.24
	and targeting control
lcrH	YopB and YopD chaperone	+	38,912	39,418	169	4.61	19.02
(sycD)
yopB	Yop targeting	+	39,396	40,601	402	7.09	41.83
yopD	Yop targeting; negative	+	40,620	41,540	307	6.80	33.39
	regulator
Orf54	Unknown	−	42,709	42,386	108	9.66	12.61
yopM	Targeted effector	+	43,481	44,710	410	4.23	46.21
Orf60	Unknown	−	46,365	45,946	140	7.79	15.81
Orf61	Unknown	+	46,637	47,026	130	7.33	14.80
sycT	YopT chaperone	−	47,468	47,070	133	4.43	15.42
yopT	Targeted effector	−	48,436	47,468	323	9.13	36.31
yopK	Yop targeting	+	48,936	49,484	183	4.37	21.00
(yopQ)
ylpA	pseudogene	+	50,089	50,718	210	5.80	22.40
sopA	Plasmid partitioning; negative	+	52,730	53,896	389	5.82	43.41
	regulator of sopAB transcrip-
	tion
sopB	Plasmid partitioning; binds to	+	53,896	54,858	320	10.19	35.61
	sopC region
Orf73	Unknown	+	56,087	56,362	92	6.16	10.10
Orf74	Unknown	+	56,355	56,654	100	5.51	11.67
Orf75	Unknown	−	56,792	56,496	99	9.88	11.19
yopE	Targeted effector; causes	−	57,453	56,794	220	6.59	22.99
	actin depolymerization
sycE	YopE chaperone	+	57,647	58,039	131	4.49	14.65
(yerA)
sycH	YopH chaperone	+	60,796	61,221	142	4.81	15.76
Orf84	Unknown	−	62,897	62,568	110	8.98	13.00
Orf85	Unknown	−	63,500	63,036	155	4.97	17.71
yadA′	pseudogene	+	67,532	67,783	84	5.21	8.92
′yadA	pseudogene	+	67,900	68,835	312	6.84	32.47
a ORFs within transposable elements as well as disrupted or partial ORFs (except for ylpA, yadA′, and ′yadA) are not included in the table.
b Except for copB, yopN, yscV, and yerA, all alternate gene designations, in parentheses, are Y. enterocolitica terminology; copB - plasmid R100 terminology; yopN - Y. enterocolitica and Y. pseudotuberculosis terminology; yscV - proposed terminology change; yerA - Y. pseudotuberculosis terminology

New Potential Virulence-related ORFs

Fourteen ORFs are not obviously associated with IS elements and either have no significant similarity to proteins in the database with known functions or have features suggesting a virulence-related role. These are ORFs that deserve future study as potentially having virulence or virulence-accessory functions.

ORF75 (Table 3) lies just 1 bp downstream of yopE and lacks an obvious ribosome binding site or upstream promoter. The ORF could encode an 11,192 Da protein with at least one likely transmembrane domain and a noncleavable signal sequence. Its expression conceivably is translationally coupled to that of yopE suggesting that it could be a member of the LCR. yopE has been called monocistronic, based on its estimated transcript size (750 bases in Y. pseudotuberculosis ). The presence of this ORF has not been noted in the literature, even though the beginning of Orf75 is present in the sequences previously submitted for Y. pseudotuberculosis yopE , Y. enterocolitica 0:9 and Y. pestis EV76 . Interestingly, it is intact but separated from yopE by an insertion element in Y. enterocolitica 0:8 strain 8081 . At high doses, a Y. pseudotuberculosis mutant containing an insertion in this ORF did not cause loss of virulence in mice infected orally (Forsberg, et al. J. bacteriol . 172:1547-1555, 1990). Given that YopE's importance in virulence was determined with polar insertion mutants, the significance of this ORF needs to be thoroughly tested.

While assembling this data, we learned that two new ORFs we found in Y. pestis have been designated as YopT and SycT in Y. enterocolitica (Miller, et al. J. bacteriol . 172:1062-1069, 1991). sycT and yopT are arranged in what appears to be a bicistronic operon upstream 500 bp and on the opposite strand from yopK (FIG. 2 ). These genes indeed have properties suggestive of a Yop and associated Syc. sycT is predicted to encode an acidic 15.42 kDa peripheral protein (Table 3). The database search brought up weak homology with SycE (with which there is 22% identity). Alignment of SycT with SycE, LcrH (SycD), and SycH shows the greatest similarity toward the C termini of the proteins, as previously demonstrated in a comparison of SycE and LcrH/SycD . YopT is predicted to be a peripheral 36.31 kDa basic protein (Table 3). It shows 36.7% identity in residues 98-322 with the C-terminus (residues 648-874) of a surface antigen in Haemophilus somnus that is associated with serum-resistance . The regulation, mechanism of action, and role in plague of YopT should be investigated.

ORFs 42, 43 and 44 (Table 3), located immediately downstream of tyeA (FIG. 2 ), have been noted to exist in Y. enterocolitica (Winans et al. J. bacteriol . 154:117-1125, 1083). ORF42 has been sequenced in Y. pseudotuberculosis and a polar insertion near its 3′ end caused a calcium-independent growth phenotype (Forsberg, et al. Mol. Microbiol . 2:121-133, .1988), typical of mutations in genes necessary for the functioning of the type III secretion system. Because this mutation was complemented by DNA lacking a complete lcrD/yscV gene (downstream of ORF44), the phenotype is not likely to be caused by disruption of lcrD/yscV. This, taken together with their location (within the LCR cluster and downstream of tyeA, which is involved in Yop secretion control), suggests that one or more of the ORFs 42 through 44 have a role in secretion or secretion control.

ORF5 (Table 3) is isolated from other virulence-related genes, within a gap between the origin region and an IS1236 remnant. It is presently unknown whether the sequence. encodes a virulence-related factor.

ORFs 59, 60, and 61 (Table 3; FIG. 2 ) lie between yopM and sycT. Orf59 is closest to yopM (242 bp away), on the opposite strand, and is predicted to encode a 4 kDa soluble acidic protein (Table 3), which is significantly smaller than typical Sycs. Orfs 60 and 61 lie 875 bp from Orf59, are separated by 272 bp, and are divergently oriented. Both are predicted to encode membrane-associated proteins with mildly basic pIs that hence do not resemble typical Sycs (acidic, soluble, ca. 16 kDa) or Yops (soluble). Orf 60 has an uncommon translation initiation codon (leucine) (Table 3).

ORFs 73 and 74 (Table 3) lie in the vicinity of yopE. The predicted proteins are 10-11 kDa soluble acidic proteins that show high similarity to unknown proteins of similar lengths in Mycobacterium tuberculosis ; however, neither ORF has a common translation initiation codon (leucine [ORF73] and valine [ORF74]). Both ORFs are predicted to be transcribed in the same direction, with Orf74 overlapping Orf73 by 8 bp (Table 1).

ORFs 84 and 85 (Table 3; FIG. 2 ) occupy the region between IS1617 and Tn1000p. They are separated by 139 bp and would be transcribed in the same direction. The predicted product of Orf84 is a basic soluble protein and the product of Orf85 is predicted to be an acidic soluble protein (Table 3).

We identified a number of intact, defective, and partial IS elements in pCD1. The site of an IS100 insertion, an element with numerous copies in the Y. pestis genome (Fetherston, et al. Mol. Microbiol . 13:697-708, 1994; Portnoy, et al. Infect. Immun . 43:108-114, 1984), was confirmed and refined. Two new IS elements, which we have named IS1616 and IS1617, were discovered ( FIG. 2 ) and were registered through Dr. Esther Lederberg Plasmid Reference Center, Stanford, Calif. In addition, numerous IS element remnants were identified; these partial Iss primarily cluster in four regions of pCD1 (discussed below).

It is curious that IS100 is nearby one end of the yscM to yopD LCR cluster and two partial IS285 elements bound this same region (FIG. 2 ). The type III secretion system and regulatory genes, exemplified by this LCR cluster, is widespread among bacterial pathogens and has been suggested as a possible pathogenicity island (PAI) . PAI hallmarks include carriage of virulence genes, a distinct GC content compared to the host bacterium, a discrete genetic unit often flanked by direct repeats, association with tRNA genes and/or insertion sequences, presence of “mobility” genes (transposases, etc), instability, and absence in less pathogenic strains . An additional requirement of a chromosomal location may be somewhat artificial given the large sizes of many virulence plasmids. Although the LCR cluster does have IS elements associated with it, we failed to detect any tRNA genes anywhere on pCDT. In addition, the LCR cluster does not contain effector Yops (except for lcrV). Finally, the GC content of this region (44.8%) matches that of the entire plasmid and is similar to the 46-47% GC content of the genome of Y. pestis.

Insertion elements. Several mobile genetic elements have been found in the pathogenic yersiniae and most of them are present on LCR plasmids as well as the chromosome . ISs known to be associated with the LCR plasmid of Y. pestis include IS100 and IS285 Additional elements are found on the LCR plasmid of Y. enterocolitica but are not present on the Y. pestis plasmid Sequence analysis of pCD1 from Y. pestis KIM5 revealed the presence of three complete insertion elements and numerous partial IS elements. Complete and partial IS elements with >85% identity at the DNA sequence level were considered to be the same as previously described IS elements. For the remaining elements, the highest database match at the aa sequence level was considered the closest relative. Only complete IS elements were given new IS number designations.

An intact copy of IS100 is located downstream of yopH in pCD1 (FIG. 2 ). There are numerous copies of IS100 throughout the genome of Y. pestis KIM strains; the IS100 element (bp 12,609 to 14,562) in pCD1 (bp 12,609-14,562 of SEQ ID NO:1) is 100% identical in size and nucleotide sequence to a copy of IS100 present on the pesticin plasmid of Y. pestis strain EV76-6. A five base pair direct repeat flanks the IS100 which appears to have inserted within the relic of another insertion element. Five and seven base pair duplications have been found flanking other IS100 elements in Y. pestis.

IS1616 is a new 1,254 bp insertion element located at bp 50,753 to 51,987 of the entire assembled sequence, between ylpA and the sopABC partitioning region. The inverted repeats at the ends of IS1616 are 40 bp long and contain 9 mismatches. No direct repeats were detected flanking this element. While some elements do not generate a direct repeat upon transposition, the absence of direct repeats could be indicative of changes in the flanking DNA as a result of mutations that have occurred over time. There are three open reading frames within IS1616, the first ORF (OrfA, bp 50,825 to 51,142) is predicted to encode a protein of 105 aa with a pI of 12.6. A second ORF of 186 aa (OrfB, bp 51,064 to 51,624) overlaps OrfA in the −1 frame. An additional 101 aa (orfc, bp 51,625 to 51,930), which may have originally been part of the second ORF, are encoded in the same frame just past the stop codon at bp 51,622 for OrfB.

IS1617 is a new 1,214 bp element, with inverted repeats of 39 and 40 bp containing 13 mismatches, located downstream of sycH. The five bases flanking each end of IS1617 are identical in 4 out of 5 positions. Like IS1616, this element belongs to the IS3 family and contains 2 overlapping ORFs with OrfB in the −1 frame relative to OrfA. OrfA could encode an 88 aa protein (bp 62,202 to 62,468, complement) while OrfB is open for 289 aa.(bp 61,369 to 62,238, complement). A potential translational frameshift window of AAAAAAG is present in OrfA. IS1617 is more closely related to IS1222 from Enterobacter agglomerans and to ISD1 found in Desulfovibrio vulgaris than to IS1616. A remnant of IS1617 is present downstream of yopj in pCD1 as well as in Y. pseudotuberculosis pIB1.

We found no evidence for the existence of yopL and, in Y. pestis , ylpA and yadA-are pseudogenes. Although regulatory and secretory components of the LCR constitute a contiguous LCR cluster, elements suggesting this region is a pathogenicity island were not identified. Effector Yops are scattered throughout the plasmid and have widely varying GC contents, indicative of multiple gene acquisition events. This observation coupled with the presence of IS remnants from only distantly related microorganisms suggest a very complex history of DNA acquisition, insertions, deletions, and rearrangements was required for assembly of pCD1.

We failed to find genes with similarities-to putative virulence factors that are not potential members of the LCR. However, we did identify eight ORFs of unknown function (Orfs 5, 59-61, 73, 74, 84, and 85). Orfs 7, 42-44, and 75 as well as YopT and its chaperone SycT are potential new members of the LCR virulence system. Sequence analysis of Orf7 suggests that it could be a chaperone for YopJ. Further investigation of these Orfs will allow assignment of their functions as LCR members or non-LCR virulence determinants.

We corrected the sequence of yopM, showing that it has two additional LRR repeats that are absent in Y. enterocolitica . While most LCR-related Y. pestis gene products showed 98% identity to their analogous Y. enterocolitica gene products, YopJ, YscG, YscE were ˜94% identical to Y. enterocolitica products. It will be necessary to determine whether any of the differences in YopM, YopJ, YscG, YscE and the lack of a functional YlpA gene product are involved in differing levels of virulence among the pathogenic yersiniae.

An analysis was also done of the ORFs present in pPCP1. This analysis is presented in Table 4 below.

TABLE 4
Gene ID	Coords.	Genpept	Gi#match	Description of Match
Y0002	971 > 1165	gi|455143	RNA I	inhibition modulator protein (rom)
Y0003	1532 > 1903	gi|144312	ORF	[Plasmid ColE1]
Y0004	2389 > 2826	gi|1200166|gnl|PID″e223344		pesticin immunity protein [ Yersinia pestis ]
Y0005	2861 < 3934	gi|984824		pesticin [ Yersinia pestis ]
Y0006	4052 > 4468			unknown
Y0007	4711 > 5649	gi|155525		plasminogen activator [ Yersinia pestis ]
Y0008	5836 < 6135	gi|1806206|gnl|PID|e293663		unknown [ Mycobacterium tuberculosis ]
Y0009	6135 < 6482			unknown
Y0010	7312 < 7686			unknown
Y0011	7743 > 8765	gi|1655837	ORFA;	putative transposase [ Yersinia pestis ]
Y0001	8762 > 9544	gi|1655838	ORFB;	putative transposase [ Yersinia pestis ]--

Thus the genes Y004, Y005 and Y007 are of particular interest as targets for use in treatment strategies due to their relationship with pathogenicity.

3 1 70559 DNA Yersinia pestis 1tttactctgc ttcgctgaat tttcgggttg atgtaaaacg tctgcaactg cgtgaattgc 60atcaacagag ccggggagca gccggcagca gaacactgag tctgctgatg cgtcagtcgg 120gttataacgt ggtgcgctgg ctggcccgca ggctgatgcg ggaatgtggt ctggcgagtc 180gccaacccgg aaaacctcgt taccgtggcg aacgggaggt gtcactggca tcgccagact 240tactgaaaag gcagtttaag ccgtcggagc ccaatcgtgt gtggagtgga tatatcagct 300atatcaaagt caatggtggc tggtgctacc tggcactggt gattgacctt tactctttcc 360attggtgagt tatccaggca gttacccctg cggctcatac tctgcatcac tccgttcctc 420cacagtaact gcctgtattt cttactcctg tactgccctc cttgatccga atgaaacagc 480agcctctttt cccttgggcg cgtctccagt gcattacgcc aggctcgaca caccagctca 540gcatccgggg atgacgatat ggcactgccc actatccgac gggagtaaac cgaagcctga 600aaagtgaatg gctgcctgta gggggctata tggatgtcca tcatgcggta cgagatatcg 660gtgaatggat acaaagttat tacaacacac ccccatcggc acaatggtgg attaccgccc 720tgtgaatacg aagagcggtg gaaaaaggct acgaaggtgt cctgattttg tgatccacta 780caggatggat ctgttggtca atgccggcat tcctgtccga actgtcaaca gtttcaaagc 840gcttcacgac aaggtgatta tcgttgatgg caagaatacg cagatggggt cgttcaattt 900tagccaagca gccgtacagt ccaactcgga gaatgtgctg attatatggg gggacttcac 960ggtggtacag gcgtatctgc agtattggca gtcacgctgg aataaaggaa ctgactggcg 1020ttcgtcgtac tgatctcact cttttcgctg agtcattaat gtgatccgtg cgccaacacg 1080ggttatgtct gcaaaagtta agtcgctgat ttcataaatc tgttgtattc tctgcgaaac 1140gatccttgtt tgatctttga gggggctgat atgtcgcaga tcgaaaatgc agtaacttcc 1200tcatcgaaac gtgcctacag aaaggggaac ccgttaactg gagccgagaa acaacgtatg 1260tctgtttccc ggaaaaaaga gacacataaa gctataaatg tgttcattca gaacgatctc 1320aaaaacgaac ttttacaact ctgtgaagat tcaggtttga ctcagacaga aatgattgag 1380cgctggattc agagagaaaa ggccgctaga actaatgcag cttgaatggc aactaagtta 1440ctttcttgat ccttcaggca gtgagtgcta gattaccgat tgtttaaaga atttttggct 1500ggccacgccg taaggtggca gggaactggt tctgctaagg tgtttacttg gaaccagaaa 1560agcaaaaacc ccgataaact tcctcatctt tggcgaggcg agaaggttac cggggcccac 1620ttaaaactgt atagaagctg ttgctctata cagggagtat atgtgcatgt tcagaaaagt 1680tcaatacctt ctgcgcttgt tactccttcc gtgcaacata agtgcgggaa ggtgtgacta 1740accaccaggc cctgttcaca catcattatc gacaggttaa aaacccgaat ccggaattta 1800cgccgcgaga gggtaaaaaa accctgccgt tctgccgtaa gctgatggcg aaagccgaag 1860gcttcacatc tcgttttgat ttctccatgc acgtcgcatt cgcgcgttct ctgagtttgc 1920gtcatcgtat gccgccgtta ctacgtcggc gtgctatcga tgcattactg cagggtatgt 1980gtttccacta cgacccactg gccaaccgta tccagcgctc gattaccaat ctggccattg 2040agtgtggtct ggcgacagag tcaaagagtg gcaacctttc catcacccgc gccacgcgtg 2100cgctgcgctt tttatctgag ctggggctga ttacctacca gaccgaatat gatccacaga 2160ttggctgtaa tattccgact gatatcacgt tcacaccggc gctgttttct gcgttggatg 2220tgtcggacgt tgctgtcgca gcagcccgac gcagccgcgt tgaatgggaa aatcagcaaa 2280gggagaaaca acgtctgcct agattggaga tggatgagct gatagcaaag gcatggcggt 2340tcgtccgcga gcgtttccgt agctatcaaa ctgaacgtaa ggctcatgga ttgaaacgtg 2400cccgtgcgcg ccgtgatgtt gaccgtacgc gccgtgacat tgaagctatc gttaaccggc 2460agctgacgcg tgagatagct gaggggcggt ttgtcggtaa tctggatgcg gtacgcaggg 2520agaaagcccg tcgcgtgaag gaacgcatgc tgatgtccag gaacaataac tacacccggt 2580tggccaccgg cgctacctga cgtcgtattt actgaaaatc cggttagcgc cggaggattt 2640cgctcgtctg cctcctgtca gtgcttgtta tttgagcgat gtgacggccg ctacatgacc 2700tatcttctct ttcccgccct gaaaatgccc tcgaattctc ccaatatcgt cgcttgaatg 2760tactgattta ggttatccac agttaactgc aagggaactt cccataaagt tacaaccgat 2820atgtttttta agcgccagcc gaacttgttt taaagtgcgt ggtttctttt aaaccactga 2880tcagcacttc ttttaaatac cttttctttt attcctataa ataaataacc tattcgtcgt 2940ctgccttttg gacagactat gataatgccc gcccttgcag cgaacttacg ttcgcgccgg 3000ttcggaaaca aagaaatacc tcctatcacg atgctacgag cgcataaacc cttattagtt 3060acaacattca cgattcgacc aaaaaaatac cagaccgcat acatctgaga ccactgcgcg 3120cacccttacc cactaaaaag ccgccccgcc ccgggccaac ggcccggaac agagtggctt 3180tacaatgagt gttgtaacta aaattttcga gtcgctgcaa gtcatgtcgc tggaagtcat 3240tcgaacacgc tcgtaagcgg ccctaatggc ccgctaacgc ggagatacgc cccgcctgcg 3300gcaaagcctt gtcgggacca ctccgaccgc gtaatgaagc acctacgcta cttttagtgg 3360gtttagcttt gcagggagga gattgggatt ggtgaaacct atcaaaccgg aaccggctac 3420gccgggctat cggcgccaat tgtcaacaga gttatagtta atatccgctg gcgccttcat 3480gccgcgatgc ggcattgaaa agaccggctg cgcactgctc gcctgtccgg taaggacatg 3540atataaacgt ttgtgtttat aagcactttt gtggatatcg ttatgcagct gagtgactac 3600attgacagtg tatacggaac agccgtaagc gagcggcgat ctcatattgt taactcactt 3660ccgttatcac actctggtct cagtcaatac aacgaacctg aggtgaaata tgacgacacg 3720gtattctcgt agtgaacggc agcaccatct cgacgcctgg caatagagct gaatgtctaa 3780aaaacactac tgtcggctgc atgacttgaa catcgccacc ttttattact ggctcaaaca 3840tcatcaagat gacaccaccg ttgccattcc ccctgcgttt atccccgctc gccgggtaat 3900accagacaat aacggtaccg aggcagtgac cctcaacctc cccaatggct gttcggtcag 3960ttgtcttcct gctcagttac gcgctgttat gcaggcctta tccctatgtt gacgccacaa 4020cacatctggt tggcacgcga gccggtcgat atgcgtcgcg gtatcgcaat acattaccga 4080ccatctgcat cagccctggc aaggcgaagc cgcctttgtc ttttgcaaca aaggcgcgtt 4140cgcgtatcaa agttctgcgt tggaacaaac acggggtctg gttgtgaaga ggctgctgtt 4200tcattcggat cagggagggc agtacaggag taagaaatcc aggcagttac tgtggaggac 4260cggagtgatg cagagtatga gccgcagggg taactgcctg gataactcac caatggaaag 4320agtgttccga agcctgaaaa gtgaatggct gcctgtaggg ggctatatgg atgtccatca 4380tgcggtacga gatatcggtg aatggataca aagttattac aacacacccc catcggcaca 4440atggtggatt accgccctgt gaatacgaag agcaggggaa aaaggctacg aaggtgtcct 4500gattttgtga tccactacag caacggaata gctccgctca gttatctgac ggacagcttc 4560ttccttaaat tcaggggtaa aacgtggtgt gcccatagac tcctcctatg cttaagatat 4620aggtcagatt tgtctacagg ttcgggggct ccccaaatat ctacaataac taaaaatcag 4680tggctggaag tgatatattc tgggacgggt ttaatcaatg atagatatca ccgtaaatca 4740gattaaagag cttttgtgtg attaacacca cctttactga gttactccca aaaatagcaa 4800gtcactttgg attagataaa ttgagccaag atgaatatgg cttgtgtgag cttatcctca 4860acgaccgagt cgttattatg ctgagggctg atgaaatatt gaatcgattg actctgttgg 4920ggccaatctt aggattttct ggaccagagg cgcgcagcgc cgctagtcag ctttttttct 4980gttatagcat caatgccttg aataaggacg gcccttgttt cgcttggagt gaagaactgg 5040ggctgatcgc attcaagcac ctttctctcg acgagctgaa tgttgagaac gttagcaagg 5100agatagcgaa cttttacgac tggttgagct tggtcagttt accagcagaa actgcccctt 5160catactcaat ctactcaatc ggttaaatgg ggatgagtaa agcatgaaaa gcgtgaaaat 5220catgggaact atgccaccgt cgatctccct cgccaaagct catgagcgca tcagccaaca 5280ttggcaaaat cctgtcggtg agctcaatat cggaggaaaa cggtatagaa ttatcgataa 5340tcaagtgttg cgcttgaacc cccacagtgg tttttctctc tttcgagaag gggttggtaa 5400gatcttttcg gggaagatgt ttaacttttc aattgctcgt aaccttactg acacactcca 5460tgcggcccag aaaacgactt cgcaggagct aaggtctgat atccccaatg ctctcagtaa 5520tctctttgga gccaagccac agaccgaact gccgctgggt tggaaagggg agcccttgtc 5580aggagctccg gatcttgaag ggatgcgagt ggctgaaacc gataagtttg ccgagggcga 5640aagccatatt agtataatag aaactaagga taagcagcgg ttggtagcta agattgaacg 5700ctccattgcc gaggggcatt tgttcgcaga actggaggct tataaacaca tctataaaac 5760cgcgggcaaa catcctaatc ttgccaatgt tcatggcatg gctgtggtgc catacggtaa 5820ccgtaaggag gaagcattgc tgatggatga ggtggatggt tggcgttgtt ctgacacact 5880aagaaccctc gccgatagct ggaagcaagg aaagatcaat agtgaagcct actggggaac 5940gatcaagttt attgcccatc ggctattaga tgtaaccaat caccttgcca aggcaggggt 6000agtacataac gatatcaaac ccggtaatgt ggtatttgac cgcgctagcg gagagcccgt 6060tgttattgat ctaggattac actctcgttc aggggaacaa cctaaggggt ttacagaatc 6120cttcaaagcg ccggagcttg gagtaggaaa cctaggcgca tcagaaaaga gcgatgtttt 6180tctcgtagtg tcaacccttc tacattgtat cgaaggtttt gagaaaaatc cggagataaa 6240gcctaatcaa ggactgagat tcattacctc agaaccagcg cacgtaatgg atgagaatgg 6300ttatccaatc catcgacctg gtatagctgg agtcgagaca gcctatacac gcttcatcac 6360agacatcctt ggcgtttccg ctgactcaag acctgattcc aacgaagcca gactccacga 6420gttcttgagc gacggaacta tcgacgagga gtcggccaag cagatcctaa aagataccct 6480aaccggagaa atgagcccat tatctactga tgtaaggcgg ataacaccca agaagcttcg 6540ggagctatct gatttgctta ggacgcattt gagcagtgca gcaactaagc aattggatat 6600ggggggggtt ttgtcggatc ttgataccat gttggtggca ctcgacaagg ccgaacgcga 6660ggggggagta gacaaggatc agttgaagag ttttaacagt ttgattctga agacttacag 6720agtgattgaa gactatgtca aaggcagaga aggggatacc aagaattcca gtacggaagt 6780atccccctat catcgcagta actttatgct atcgatcgtc gaaccttcac tgcagaggat 6840ccagaagcat ctggaccaga cacactcttt ttctgatatc ggttcactag tgcgcgcaca 6900taagcacctg gaaacgcttt tagaggtctt agtcaccttg tcacagcaag ggcagcccgt 6960gtcctctgaa acctacggct tcctgaatcg attaactgag gctaagatca ccttgtcgca 7020gcaattgaat actctccagc agcagcagga gagtgcgaaa gcgcaattat ctattctgat 7080taatcgttca ggttcttggg ccgatgttgc tcgtcagtcc ctgcagcgtt ttgacagtac 7140ccagcctgta gtgaaattcg gcactgagca gtataccgca attcaccgtc agatgatggc 7200ggcccatgca gctattacgc tacaggaggt atcggagttt actgatgata tgcgaaactt 7260tacagtggac tctattccac tactgattca acttggacga agcagtttaa tggatgagca 7320tttggttgaa cagagagaaa agttgcgaga gctgacgacc atcgccgagc gactgaaccg 7380gttggagcgg gaatggatgt gacaagtgcc ccctaagcct tgagttgata tatccgagaa 7440taggttaaga tttggcaatt gcttaacaat aattattttc ttattaaaaa tacctaacac 7500aaaaaatacg ttatatatac aaatgaaaat ttccagtatt aatctcaaca agtttctcta 7560ccggagaata ttaatctgga atgtgtaata gagaaaattt ttgatgctat caaattttcc 7620ttttttgcag aaatatccaa tgtataggta tgataggagt tattgggaat ttttgttcga 7680gtgctgcccg tctgttccgg gttcacccat caacgattga acgtcttatt gcaatgtacc 7740gtttatctgg aatataaaat tcataccgct gttaattccc tgaataagga taaataaatg 7800atcggaccaa tatcacaaat aaatatctcc ggtggcttat cagaaaaaga gaccagttct 7860ttaatcagta atgaagagct taaaaatatc ataacacagt tggaaactga tatatcggat 7920ggatcctggt tccataaaaa ttattcacgt atggatgtag aagtcatgcc cgcattggta 7980atccaggcga acaataaata tccggaaatg aatcttaatc ttgttacatc tccattggac 8040ctttcaatag aaataaaaaa cgtcatagaa aatggagtta gatcttcccg cttcataatt 8100aacatggggg aaggtggaat acatttcagt gtaattgatt acaaacatat aaatgggaaa 8160acatctctga tattgtttga accagcaaac tttaacagta tggggccagc gatgctggca 8220ataaggacaa aaacggctat tgaacgttat caattacctg attgccattt ctccatggtg 8280gaaatggata ttcagcgaag ctcatctgaa tgtggtattt ttagtttggc actggcaaaa 8340aaactttaca tcgagagaga tagcctgttg aaaatacatg aagataatat aaaaggtata 8400ttaagtgatg gtgaaaatcc tttaccccac gataagttgg acccgtatct cccggtaact 8460ttttacaaac atactcaagg taaaaaacgt cttaatgaat atttaaatac taacccgcag 8520ggagttggta ctgttgttaa caaaaaaaat gaaaccatcg ttaatagatt tgataacaat 8580aaatccattg tagatggaaa ggaattatca gtttcggtac ataaaaagag aatagctgaa 8640tataaaacac ttctcaaagt ataatgtatt ttggaaatct tgctccagta tgggaatacg 8700gttcagttct ttctggctca tggtcaccaa catagacgct tcggattgcc tgcctgtgaa 8760gaaacagatt aactggggtt ctacgccgga atcccagatt tttccgtcac cccagtttca 8820gcgctgctag agtacgggtg gtatgagccg ctagcagaag ctctaaatag taacttcttc 8880caatggccga aaaagaaagc gttaaaaaat cacagtacgg gcatttctcg ggtttacgtt 8940atttgtgcag aacgcacaaa tcaggttatt agatattatt gcttatgaac gggtagtatt 9000cagcgaaata cagctcctaa ataactgcgc caaatagtag atcactgagg gaactcaatc 9060cggtttaagc gatctgatca atcgctgaat atcccaaatc accacaaccg gactgagtta 9120tgccgatcat agcaccgata cccagaaata aacgacatca gatggaaaaa attgtccata 9180aaacagcaga caaaaaccat tccagacatc tcatcgctga tccctcccca atatccgtac 9240caggctaaat cagagatccg gacctttttg atgacttcgg gcaaattctg ccggagtcag 9300gttatttaac gaagaatgcg gacgaaaatg attatattct tgccgccatt gttcaatttt 9360ctcctgagca tcttccagag aaaggaaccc gtgcacgttc agacattcat ccctcagact 9420gccattaaat gactcgataa aggcattatc tgtaggcttt ccggggcgtg aacagtccat 9480cgtgaccctg ttttcatacg cccatcggtc catcgacttc gagatgaatt cgctgccgtt 9540atctgtctgc agcctttgtg gaatacgccc cagcgaatgt tttaatctgt ccatgacagc 9600cacaacatca tctccacgta acccctgacc gacctcgagc gccagacatt cacgactaaa 9660attatccact atagttagcg ccctgacccg atgcccgttg aacagattat cagcaacgaa 9720atccatgctc cagcactgat ctaacgcggt cacttctgga cgtgcgtgcc tgtgcctcgt 9780tgtcacatgc cgccgtggac gtttcgaacg tagattgaga ccctcaagat aatcgcacgg 9840aaaactgcat ccgtccggtg gccgtaggcc gcaagaactg gttgttcgca gggtcattgc 9900gtgccgggca acggatggcg tccatcctga gtctgctgga aaccgccaaa ctcaacggcc 9960acgaccctta tgtctggctg cgcgatgtac tgacccgctt gccgacctgg cccaacagcc 10020agctcaacgc gctgctacct tacgccgaaa accgcttcag ctaattaccc cgccagctta 10080ttgcattatt ttaatcgagc aacgcgagtt caccgttcgg ttacagtatt accatctgtt 10140cccgcttaat tttttaaaaa atttaaggta acaatgagta tatatcttat gggaaaagcc 10200aaaaaactaa cgaacactat aataattcga ttaacattaa tgaaaataca cggctcacct 10260attattaaaa taatacgact agcattataa gaaaaaatat tttttatgtt tatagtatag 10320gcgtgtattt aattaaggag ggaagcatga acttatcatt aagcgatctt catcgtcagg 10380tatctcgatt ggtgcagcaa gagagcggtg attgtaccgg gaaattaaga ggtaacgttg 10440ctgccaataa agaaactacc tttcaaggtt tgaccatagc cagtggagcc agagagtcag 10500aaaaagtatt tgctcaaact gtactaagcc acgtagcaaa tgttgttcta actcaagaag 10560ataccgctaa gctattgcaa agtacggtaa agcataattt gaataattat gacttaagaa 10620gtgtcggcaa tggtaatagt gtacttgtca gtttacgtag tgaccaaatg acactacaag 10680acgccaaagt gctgttggag gccgcattgc gacaagagtc gggagcgagg gggcatgtat 10740catctcattc acattcagcc cttcacgcac cgggaacccc ggtgcgtgaa ggactgcgtt 10800cacatctaga ccccagaact ccaccgttgc caccgcgtga acgaccacac acttctggcc 10860atcacggggc tggcgaagcc agagccaccg caccaagcac tgtttctcct tatggcccag 10920aagcgcgcgc agaactcagc agccgcctca ccacattgcg caatacgctg gcgccagcaa 10980cgaatgatcc gcgttactta caagcctgcg gcggtgaaaa gctaaaccga tttagagata 11040ttcaatgctg tcggcaaacc gcagtacgcg ccgatcttaa tgccaattac atccaggtcg 11100gtaacactcg taccatagcg tgccagtatc cgctacaatc tcaacttgaa agccatttcc 11160gtatgctggc agaaaaccga acgccagtgt tggctgtttt agcgtccagt tctgagatag 11220ccaatcaaag attcggtatg ccagattatt tccgccagag tggtacctat ggcagtatca 11280ctgtagagtc taaaatgact cagcaagttg gtctcggtga cgggattatg gcagatatgt 11340atactttaac gattcgtgaa gcgggtcaaa aaacaatctc tgttcctgtg gttcatgttg 11400gcaattggcc cgatcagacc gcagtcagct ctgaagttac caaggcactc gcttcactgg 11460tagatcaaac agcagaaaca aaacgcaata tgtatgaaag caaaggaagt tcagcggtag 11520gagatgactc caaattacgg ccggtaatac attgccgtgc gggtgttggc cgtactgcgc 11580aactgattgg cgcaatgtgc atgaatgata gtcgtaatag tcagttaagc gtagaagata 11640tggtcagcca aatgcgagta caaagaaatg gtattatggt acaaaaagat gagcaacttg 11700atgttctgat taagttggct gaaggacaag ggcgaccatt attaaatagc taatgtaaat 11760atttattcct atgagtaaat aaaattacta agagatatac accactttgc caatcaaaga 11820aactttaaac ctcaactaaa gtaagcaatt agttgaggtt tatctgctgt agaataattt 11880ttaacaaaaa tataaacaac aaaattaaaa gttatgtgtc tactttatgt aaccaaacga 11940gcctgtccat aattctgtgt aatcgccact gtattaaagg tgatcgttta gacggtcacc 12000gaactcgata ataaaacgac tcattgccaa ccgccagttt tgtattggca tgctccattt 12060tttcgaagca tcccggatag ccagataaat aaccttccgc accgagtcgt ccgtcgggaa 12120tactttgcat ttctttatcg cctgccggat cacactgttc agtgactcaa tggcattcgt 12180ggtgtagatg gccttgcgga tatcgggcgg atagccgaag aaggtattga gattttccca 12240gtgtgtatgt agtccctcct atttttagta ccaccgccag tgaggatctc catccagttt 12300ttgtcttgca tagataacgg gtggcatatt acccagtgag ctatgcgggc gttcttcgtt 12360atattccgtt cgccagtctt ctgtaagcgt acggacttcg gacagtgaac ggaacaaata 12420catatcaagt atcactccgt gatgttctgc ccattcaacc agtgctgcag caataaattc 12480tggaccgtta tcactccgta taaaagcagg atagcctctt tctgtactta atcgctccaa 12540aatacggacc actcggtgta ccggtatatt caagtcaatt tcaattgcca gtgcttcccg 12600gttaaaattg taacgaacgg tgcaatagtg atccacaccc aacgcctgaa atcagatcca 12660gggggtaatc tgctctcctg attcaggaga gtttatggtc acttttgaga cagttatgga 12720aattaaaatc ctgcacaagc agggaatgag tagccgggcg attgccagag aactggggat 12780ctcccgcaat accgttaaac gttatttgca ggcaaaatct gagccgccaa aatatacgcc 12840gcgacctgct gttgcttcac tcctggatga ataccgggat tatattcgtc aacgcatcgc 12900cgatgctcat ccttacaaaa tcccggcaac ggtaatcgct cgcgagatca gagaccaggg 12960atatcgtggc ggaatgacca ttctcagggc attcattcgt tctctctcgg ttcctcagga 13020gcaggagcct gccgttcggt tcgaaactga acccggacga cagatgcagg ttgactgggg 13080cactatgcgt aatggtcgct caccgcttca cgtgttcgtt gctgttctcg gatacagccg 13140aatgctgtac atcgaattca ctgacaatat gcgttatgac acgctggaga cctgccatcg 13200taatgcgttc cgcttctttg gtggtgtgcc gcgcgaagtg ttgtatgaca atatgaaaac 13260tgtggttctg caacgtgacg catatcagac cggtcagcac cggttccatc cttcgctgtg 13320gcagttcggc aaggagatgg gcttctctcc ccgactgtgt cgccccttca gggcacagac 13380taaaggtaag gtggaacgga tggtgcagta cacccgtaac agtttttaca tcccactaat 13440gactcgcctg cgcccgatgg ggatcactgt cgatgttgaa acagccaacc gccacggtct 13500gcgctggctg cacgatgtcg ctaaccaacg aaagcatgaa acaatccagg cccgtccctg 13560cgatcgctgg ctcgaagagc agcagtccat gctggcactg cctccggaga aaaaagagta 13620tgacgtgcat cttgatgaaa atctggtgaa cttcgacaaa caccccctgc atcatccact 13680ctccatctac gactcattct gcagaggagt ggcgtgatga tggaactgca acatcaacga 13740ctgatggcgc tcgccgggca gttgcaactg gaaagcctta taagcgcagc gcctgcgctg 13800tcacaacagg cagtagacca ggaatggagt tatatggact tcctggagca tctgcttcat 13860gaagaaaaac tggcacgtca tcaacgtaaa caggcgatgt atacccgaat ggcagccttc 13920ccggcggtga aaacgttcga agagtatgac ttcacattcg ccaccggagc accgcagaag 13980caactccagt cgttacgctc actcagcttc atagaacgta atgaaaatat cgtattactg 14040gggccatcag gtgtggggaa aacccatctg gcaatagcga tgggctatga agcagtccgt 14100gcaggtatca aagttcgctt cacaacagca gcagatctgt tacttcagtt atctacggca 14160caacgtcagg gccgttataa aacgacgctt cagcgtggag taatggcccc ccgcctgctc 14220atcattgatg aaataggcta tctgccgttc agtcaggaag aagcaaagct gttcttccag 14280gtcatcgcta aacgttacga aaagagcgca atgatcctga catccaatct gccgttcggg 14340cagtgggatc aaacgttcgc cggtgatgca gcactgacct cagcgatgct ggaccgtatc 14400ttacaccact cacatgtcgt tcaaatcaaa ggagaaagct atcgactcag acagaaacga 14460aaggccgggg ttatagcaga agctaatcct gagtaaaacg gtggatcaat attgggccgt 14520tggtggagat ataagtggat cacttttcat ccgtcgttga caaaaatctc cactacattg 14580aataatctga acctccgacc gtctgtcagg gcatcactca taaaatcgac tgaccagcag 14640tggttcattt taagtggaat agccaaaggt tgtggatgcc gattgggcag gcgcttttta 14700ccttttcgcc gaaagttaag cttcagtaag cgataaacgc gatatacccg ttttacattc 14760cacggtaatc cagactgccg taacttattg aacataagac caaagccata tgccggatat 14820tgatgtgcca atttttgtaa tacctcaaca accggtatat ccctcgcggt gttaggacaa 14880taatgcagca aacttcgact gatacccata atccggcacc cgcgccgttc actggcctga 14940tattccgtca tcacgtaacg caccagttcg cgcttttcag gtaccgttaa agtttttttg 15000ccacgacatc cttaagaatt tcatgatcta aactcagaga ggcatacatc tgctttaatc 15060gtcggttttc ctcttcccgc tctttcattc gctttatatc agaggactcc atgccaccat 15120atttggattt ccagttgtag taactggctt cagatccgcc gttctcgcga cagacatcct 15180tcacatgccg gccaccttca acttctttta gaacccgcag gatctgagtt tcagtaaaac 15240gtgctttctt cataatgacc tccgctgatt atatattaac cagagaactc cattaaacga 15300taagactaaa ttcagggggg actacaagat aacggggagt ggacaatacg ttaataatgc 15360gttattcatt tcaatacgca aatgtaattt aaactaatta tcatttagat aaatacgttt 15420ttaattccaa tgccccgccg gcatggcgta aaaatataaa gtatccatcc ccccaaaccc 15480tatttattag caataaggtc agctagccca ctggtaatgc aggaatacca gagatagcaa 15540cgaatattga ctgggatgac atcaaaataa ccataacccg gcagtcgcta caatgtgatc 15600tttcatccta attctctcaa tttatatgag gagcagactc tactgataaa tacccccact 15660tcaggggatc accgaaggag gcgtattatt attctaaatt ggctcagggg aaagaattga 15720tacatgactc cttgaaaaat ggttacgtaa atcaacctgg gggatattat tgcctcaata 15780tacagtagat atatattatc tcagccgtca gccgccgtat cctggcgcat ggcggcttct 15840agcaataatt tagcatcgct aagcgagagt tgttcagtac gcaagccggt cactatcact 15900tcccctgcct tgccctgctt tagttccaaa ttagaaacgc gtggtaataa gcaggcaata 15960tcgtgacgac tgagagccgt atttttcaca tgttccagca cctcattggc aaaggctttt 16020tcggcggtag atatctgatg actattctct gtgagtgggt tagtttcagt gagacgaccg 16080gcctgccccc cgtgtcccac ttgggtaatt tgttgattta ttaacgattg aagagtattg 16140attttcatcg agtctcctgt cgctataatt gtctctacga tattctaagt tatttatttt 16200tgctaaacta ctgtcgtaga cgatttattt ttttaatcgg cttttaaaca gaaaatcaca 16260ataaaaaatt atttttggga atcattctca taaaacgagg atgaaaaatc caatttaggt 16320agataactca atcttataat agataacact aaacatatat cgatagttat tcctattctg 16380taactttcat ttgtcctaaa gtggtagata ttgcccgaga aagtgcttca atttgcccat 16440caatactcgc gtcaataata cctacctcag tctctaatat acagccccct tgatccaaac 16500gcgcatcggc agtcacctct aaatagctta tttccgggaa gtctttatgt actttagcta 16560tttgttcacg aatagctcct gcctgatcag ggttgaccct gaccacgact tgcttctgat 16620tactcaccaa ggctaaagcc tcccgcacaa cttgcagtgt catagccact tgatcatagt 16680cattgaggat tttacgtacc gccaaaagta caacttcact catctgttgt tcgacgtggc 16740gataaaattg ctgacattgt aactgtgttt catgaatcaa agtcgcctgt aaggtacgcg 16800cctcatccat gccagcctgc catcctaact gcttttgttg ctcataaacc tcttgggcgt 16860cagccaggat cttttcagca tcctgttttg cggcactaat caactcttcg gttgttaaac 16920tggattggta atcttcggcg cgcaaaatac gcagaccgca agcgagcgag agattacttg 16980gtattatttg aacaaatggc tgcatgtagg cgtaacctgt ttgacaagtt tgtgacataa 17040agtttgggcc agtgggcgtt gtgactcggc cactaaccaa ggttctgacg gcgtagctaa 17100tgggaggcgt aaactgagcc gtttacacca tgcctgaggt tggggttcca ttgctgctaa 17160ccaaaaagcc agccccgact ggatcatggt acggctctct atttctgtcg gcagcgggcg 17220ctgccagtga gtgggccaag gcccgattag cagctcatgc tgtacaataa tttgccttaa 17280tgtttcttga ttaaccaatg tcaataattg ttgtaatggg gaggccagta cacaacggcg 17340aatcgcctcc ccatgcagaa ctaatccaag gcgacaaagt aacagttcga gctgtgattg 17400aggctgcaaa ggcagcgccc ccagcccatg gggctcttca tagtcggtat caagagaaaa 17460ttcatccaat aaagcagcat tgagatgagc actatcgcgc cactgaggta agtagggcaa 17520tattgaacgc cataatgatg gtaactgttc caagtgcaaa taagccgcgg ggcagaagcg 17580caattgaaaa gaggtaatat aattttccat catcacttct tgcgttgtaa ccaaaaatat 17640tgagcaagat tggtcactgg caaaagcaaa ataagcaacg acaacaagcc aataagatgc 17700ccttttgact cttcactcac ttgaattgac agtatgctcg tgttacgagg taaatgagag 17760ctttgacgaa catctaccga tggcaccaaa atgacactga tgcgatcata ggccagcccc 17820tcaatactat tattcactaa ttgtttaatc tgaggtatgt aggtatcaaa ctgaatatct 17880gctgcatgct tgataaaaac cgaagcggat gctgctacac ccttcttacc tttgttattt 17940tgctcttcag gcaatacgac atgcactcga gccactaata ccccgtcaat ttcagataaa 18000gtgcgggaga tctcttgcgc cttggcataa ttaagcctcg ccaactcttc tatcggtgaa 18060gatatcaacc catctttggg gaacacatcc tgtaacgtgg agaaactctc gtgtggatag 18120cccttccgtt tgagaatatc aatagcctga gcgacatctg actcctcaac caagagctta 18180atcttcccat ctttgtctgg ctctttgtct gcggaaaggc cttcttggcg caacagcgca 18240agcatttcgt tcccttcctt ctgactaatt ccggtataaa gatcaacttt gcaaccagtt 18300aaaaacaaga ttaatatcaa tgttgacagt gaagtcttaa ctttcactag ttttcacccc 18360cccttcgaca aggtttcaac attttggctc attcgcccgg cagtcttggc gataagttct 18420tcttggattg ttatacggat aagtgaccat tgcattagca tcaggtcgtt gggattatca 18480actgaaacag ccagcttagt gtgtaagtca cttttaaccg tcttaaaaga cttctgaata 18540tcactaacct ccttgaggag tgaatggccc agtccctgcg tatcttctga cattgccgca 18600tcaaagcgca ttatttggtc agttgttggc tctgccggcc ctaattcctc cagcgtggtt 18660atgatcacct catcggcctg agctatttct atgttcggca tttatgtatc catatcaatt 18720tgatggctgt tatgaagtag gctatctaca atcgagttag acggtaatag gatcattaat 18780tctttattta ttaactctga taaataaggt aatccaacct ggctctcatt gggttttata 18840ccctctaata cttggagcaa tattgcttcc caccgcttac caaacgagtc aaactgctgc 18900aatacactac gcaattcagg taaatctata gccggtacaa cagacccttg atgttgaccg 18960aaacgcgcca gtaacgcctc acgtactggc gtcgccaaaa cctcaagaac ggcatggtca 19020ggagggttac tggcataata ttgctgccac agaacttccc gtgtcttttc agcggattca 19080ctttttagct tattctccag ttgtgttttc agctcagatg ctaccggttg tagcgtagag 19140actgcctgag acgaagacat caacgatgta atggaacctc tattaagggt aaccgtcatg 19200tttttagttg ctccctcatt ccattcacaa atgtctgtat tctaggatcc tgactccttg 19260cgagacgatt taaacgtgac tctaaggcgc tccccaaccc gaggcgatat tcacataagg 19320ctaaccaagg ttccaaatca ggataagcta atttatttcc ttgttgcaag gcgcttgcgt 19380agtccccacg gttcatcaaa gaggaaaggc gaatcaattg aaccgcctct tcttcacctt 19440tcaaatgtaa ccattcagca atgcaattcg cttcttcgtg gtagtggttg ccggttccaa 19500tcagagcaat ctctgctaac agtacgttga gtttatattt catattatgg gaacttctgt 19560aggatgcctt gcattaagtc tttcatgcta cgaactatgg ttgagtttat attgtaaatt 19620accgaccatt tattaattga atgttgtaag tcagcaagta gcgccgggtt gtcaggctta 19680tctttcaatg ctgctatcga gtcattaacc gctttgtttg catcgtctgc tggcttcttg 19740agcgtttgag ccaccgcatc taagtctgcg atatcggttc ctttcgtaaa tccagagaag 19800ttactcattt attttaggtc tcctgctaca taatgaataa tggctatagc tgactcaaga 19860gctttagatt ctctctgcca aacctgatac tgcttggcat caccaccgcg catcatatct 19920tttttgagct tagttagtgc catttctagt tgcatggtga tagagcgcac tgtctccacg 19980ttatgcagtt gctcctctaa ttgtgtcatc gaggtttacc tccattgagt tggatcacaa 20040attcccgttt tcctttactc acaatcaccg catcgcgtcg aatagccaga atacgaacgc 20100cattgttaag tatggcacct tctggatagc gttgatgatt gtcgagtacc acataaggca 20160ctttccctaa cgagatagct tgcacctcga aattcaattc atcatgctgg ggttgccccc 20220cgacattgac cagttctaat ttaggtcgat tgccaaactc ttggcgaaaa gtttgttgta 20280gttgattaaa tgaattcaat ttttcatcat tgacttgccc gcgtaattca atcagctcac 20340ctttaacatt tacagtgaaa tctgaatcca gaccaaattg ttcaagtaat gcatcaagcc 20400gcttgcgttg attaccggca atccgcactt tactttctac accgagcagc cctggcactt 20460cagcttgcag caggctatca attttttgct tttgtatttc ctctgacact tccccattca 20520attgtagcca tcccgcttgc ggtgctaaag aaacctcaat tccatggtat cccaaccgtt 20580gcaggatgaa ttctgccccc tgacgcagtt cttccatgct gcgcagttca agccggaatg 20640gaatgccatg gctctcaaga aaattttgca gtgacaagcg ggcatgatta tcctggatat 20700aaccagttaa taaccaaggt tcaccctctt ttttgggcga tgttaaaacg acatccttgt 20760aagcagcagt tgccagcaag cgccgtactt cttgctcaac aagttgtcca tcctggttat 20820actcgcgcca caatccgtgc cctagcagcc ccaaaaaagt caaaagcaac aataaagaaa 20880gaactccaag cccaatccca agtcgtgaac gaggtaacct gtcggttggc tcttttctct 20940gcgtgggaac ctgtaacgtc tctggcaaag gttgccctac ggcgacaaat gtccacagta 21000aaaaacctac ttccagacaa gagcccgcgc gaagaagagt ccccaacggc acgggaagcc 21060cttcttgtag tagaggttct gcagaatcag ttaggcgaat accttcttca tcgaccatca 21120gcactaaatg cacgggtgct atttcgctgt cagaaagaac aatatctgac tgcaacgggt 21180ctgaaccaaa aacacagcgc ccatgaggaa gctcaacttc aacaccacgg tgcttccctt 21240gataaaaacg acagacccaa ctcacaatac gccacgctta ggcgctgaaa cacaccatga 21300ttccgcggga gtacaagcac cttcaaccac gcgccatccc aaacttttgt ccatcttgca 21360ctgagtaaga taggatgatt tattgttttg actcagccat ttctgcactt cttgcgcttt 21420gtttaaaggc tgacactgga agccacctaa taacttattc aaggtagtgc tttgattaga 21480tatttcatca acagctaaga taccagtacg tagatcccga ccattaccta acgctaaatg 21540atgcgcaata ccttcgtcaa taatccgtgg ttcgatgata aacagccgta ccgtacggcg 21600agttaactca cttttacggc ggaaaagtgc gccaagataa ggaatatcac caagcaaagg 21660caccttacta agagcaacac tcaattcgtc acgataaata ccaccaataa tcaaactctg 21720gccatgtccc acgcgagcga cagtatcaac gaccgtacga ctgatagtgg ggattccgtc 21780aatccctgaa ctattcggtt tttggttccc atcctcaatg tgtagattga gactgatttc 21840tgacttatct ccttgagtca gcacccttgg tgtcatacgc agcatagtgc cgtaggtgat 21900ccctttcagt tcagccactt ctttacctgt cactttgacg taataggttt catggtgatc 21960aatcaccgct tgggcatttt cttgtgttag cagggtcgga cgtgaaacaa cttgagccga 22020accttcattt tcaagtaaat tgactcttgc taataggtag tcaagcccgc gagcatcaat 22080caaactaccc aatgcaccgt ttgaagcgat gttactttga tccccggttg tttttattac 22140cacctgatga ttgttgccag tacgaatgcc aactcgccag tccacaccta attcagtaag 22200ttggtcggca tttatatcga caatggataa cgccacttca atacgagcgc taggtttatc 22260aagcgcatga attaaccgtt gatacattgg catacgctca ggagaatcgc gcactattat 22320cgcattgagc gatggatccg cttcaacctt ggcttgagct gaagcccggg tagcggcctg 22380cggtattctc tgattatcca ctgtcacttg ttggattgtg gcatcgctta acacgcgttg 22440aagtatcgtt gcaaccccag gagcagccac ttcgtcatca cggtaatgaa tagttcgatc 22500gctcgctgat gcatatttga gagggaaaat ctcaatcgct aatgcccctg ttttttcact 22560gcgaatttgc gtctgttgtt ccaatgcggc tgcggtctgt tcaaccaatt caagataacg 22620aggaggacca gagacgtaaa caaggcggtt gctagcatca gggcgccagc caaaacgagg 22680ctcccatata ccagaacgtt gtaatgccag ctttaactct gcggcctcac tttcctgtaa 22740acgaatgaga cgagacgcta cctcactatt tttaaaaatg tagagcacat tgccatcata 22800gtaccaaacc aaattgtaaa gggaggcaat atgctgtagg aaatcctgag ggttatcatg 22860ctcaaactgg ccggaaactt tgtcattaat cttatcgctt actaccactg tagcatcata 22920attagcgctg aaatcaatta ataaatcgcg taaactttcc cccttcgcca cataaacata 22980aggtataggc aaccaatcaa gttcttgcgc ccagctatag ttagaaagta acagtaacgt 23040cccggtgagt acgcgcttga aaaaagaatg tagcggaaaa gccatattac ttaattccac 23100cccacgcgag acgctacaga aaatggtgtt aactgaggaa ttaaatgttc tagcagagcc 23160agttgtttat ttattacttc ttgcaatccg tgagtatcaa gctcttgtag acgtaaacgc 23220gcctccagta ccaattgacc acaatcatcc aatactaaag tttgagggta acgtttagcc 23280catgccaacg cttgttgcat tagggagcga agcaatgtga cgttaacgtt atttccttca 23340cgtaacattg gtgcgtcaat aggagtgcgt aataccagtt ctgaaccatg cggagccagc 23400atgacaagat gcttatctat agttaaacgg taaacacctt gtttatcggc aacaaacggt 23460tttcttccta aactggctgc caagtttttt agtaaatttt gcattatttc tcggatggtt 23520atatataaac taagtaatcc tgcgccaaat aaataaccta cttcatgtca tcgtgacata 23580aggtttcgcg gttcagctca aacttccaga tattcaaaaa aatgttcatg ataattatca 23640accacctgta tagcaataga tgatattacg ttaatcgaac taatgagact tgctctattt 23700gatggaggtc gtttcttgcg ccaccgcact taataaacca gataagctat attgattatt 23760aaacaacata ttattgccat ccagcggcga aacaatactg ttctatgttt cgttgaaatt 23820tggctcatcc cattgaatct tcacaatcta atcccgtatt ttactttata gtccaaaagt 23880gtcttttaaa aaaaacacag ataattttag cctgtggttg ctattttagt aagacgggct 23940tggcttggag tgcatccgaa gcgacgtcga taactttgag tgaaataaga ctgactcgag 24000aaccccgctt ccatggcaat atcaacaata ctcatcttac cattaagaag taattggtga 24060gcatagagaa tacgtcgctc gcttatccag gcgcgtggtg aaatgccata aactgtacca 24120aacagttctt tgaatgtggt taatcccatg ccgaattctc gcgcaaattt gcttagcttc 24180cacccttgta gataattttc ctccataaat ttttgcaacc gttcttctgg gcggttgcct 24240aaatggcgca gagccgagag aaataaagtc ccttgcgagc taaaggcaag caaaagcagt 24300aattcctcaa tacgcagttg cgttaatact gacggaaaat cactccgttc caatatggca 24360catagatttt gaatggattg tgataatatt ggtgaaatat taaaaattaa caatggtttg 24420ggtgtggagt tgtctcgtcc aatttcacta agcaaagaac caaagcgatg caaaaaagta 24480ctcaaaaaac tgccgggtaa tggaatccaa agtaattggc agggttcttt tgtaccacat 24540cgaacagcat agctgccacg acgcaaaaac agcatattcc cctcatctaa atcatatgtc 24600tgaccgctgc tctgccatga aatctgacct tgcaaaagaa tatataggcc atcttgtgaa 24660tgctcaacaa ccttaaatat aggtgtgacc cattctaatt taataatctc tagtgatgcc 24720ataaatgtta tactgtccta aaaatctaaa acttgtatat atttatctaa tgaggtgtat 24780tgagttatta tgcgtgcgat gtacaaccat cgattaatgc aaccaaaacc aatataataa 24840atcacctatc tggtattagg taactggcaa tttggataac atgttttagg tatcatttgt 24900aaaacgactt tgtttttacc agtaagcttt tgctgagcca ccgcctgaac tcctcgctct 24960ggaaaagaga gggttgaccg taggtaaagt tcaccttccc cgcgttgagc tggattcagt 25020tttatagaaa agaataaagg taggttacct gtttcgaaat gagtgcgctg aacctctcga 25080actttcccct catacaaccc aaacatacta acatcaatgt gtgctatgcg agataatggt 25140cgtgacatac gcacctcccc cacaatacgc tgagctggca ttgggggggt agcgcacccc 25200actaatagaa aagaaatgat gagtgctata atacgactca cgccagtccc tcccttgagt 25260tcacacaaag aagatagcta gatgatagca atccgagttc gcgcaatatg tggtgaaatt 25320tattattgta aaataacaac ccattcccaa ttatctcaac gggtccacca cgaactcatt 25380taatttagct actgcaatat cacaccttgc gcatataaag ccaaacatcc tttctttcat 25440aaaaaaagga tgtttggtta gaatcggaat cagcaaacaa acacatgtaa tagtaatgac 25500aatactgtat tatttgtatt caacaaaaaa aagtcaagag attaatccta attagtgcta 25560gttatgttta attgtaaaat gcacaggaga aatacaatta ccatactgta tatatggttt 25620taaatcgcat catatattcc taatataagt gaacctcttg ttggttaacc atatcgaata 25680aattacatat tcccaatagc cggtgttaat caccccattt ttccgataaa aacatagact 25740agaaagtaga gaaaatagta tagaccaaca aaaatgtcat ctgttttaac catattccta 25800gttacattgc agcctattat aacatttcgg aatgttgttt ctcgatattt tgcctttcta 25860gccatcgtag cacttcagct gtggcctcta tttgctcagc cggaatatag tgatcgacga 25920gcgcatccca ataaagagca cgggctaatg ggatacgttg taaaataggc accccttctt 25980cttctgctat tttgcgcaca gtctgaactt gggcatcggt atatttgaat gttaccaacg 26040gtagtggtgt ttcccctcgc ttgtaaagaa taccaatagc aatatgggtc ggattagcta 26100ccaccactga tgagcgttta acattttccc gcatgttcct cgattggatc tcttgatgaa 26160actgacgacg cttgcttttg atttctgggc taccctccat ttctttgtac tcgcgtttga 26220tctcatcctt gctcatttta agttccttaa tatattgata gtattcaaag gcatagtcgg 26280ctatggagat gaccacaaag ccaacagtac agataaccat caactgccgg agtatttgcc 26340ccaataaagg ggtaatacat tcaattccac aggttggcaa ctgcaagagt gtgactagat 26400ttcccttaat gattatccag atgagtatac tgagcaaaac aaccttgaga atggatttga 26460gaaactccac taaacttttg atggaaaaga tacgcttggc accctctatt ggattgattt 26520ttttaatatc cggtttaatt gcttcaccac ttataagaaa accatactgc acaacatgag 26580atgcgatcgc cattaatgcc gccactgtta acaaaggaaa acagagataa aaaaactcga 26640gcaacacatt gtcaaccaca tagctaagcg cctgcgagaa aggaagatag ctctgctctg 26700cggggattag catcagctta ctaaaatgct cgaaatagta gtcagaaagc cccattaaca 26760tcgcactcag cgcgacgata agcgcagtag agaccacttc cttacttttc gctacctgtc 26820cctttttgcg cgcatcacgg attttcttcg gggtgggttg ctctgtcttt tctccgctca 26880ttacttctcc aaaacaggga tcagtaaact tataggatcc ataaccaaca gcattgcttt 26940actggcatga ctcatcattt gcatacagta gataaccaac aacaggcttg ctatcgcgct 27000ttttatcggc atagccagca caaagacgtt taaagaaggc gcaaaacgac tgatgagtgc 27060aagtccaaat tcagctaaaa acatagcgat gagtaaagga gcagccaata cagcggcgat 27120taatagtatc tggctgaatt ggttataaaa gaaatcaacc cactgttcac taactgcagg 27180gaaaaagctg gccaccggcc aatttacata gctgtgaaag agggctgaaa gcaaagagag 27240gaaagcccct ccgctgaaga aaattgttat taacgtttga gtcaaaagta aaccagtcgg 27300actagtttga ctatcaagtc caggattgag tagagatgcc atcgcggcac ctctttggtt 27360atctacaata aatcctgcgg attctaaggc ccagaaagga atggtggcca caaacccaat 27420caataacccc agtatgatct ctttgccgat aagcagcatc aacgtaaacg catcaacctc 27480aatataaggt tggttagcga cggcaggata gacataaaga gccaatgaac agacaatacc 27540attacgcagc aatactccac cgaggagctg tttacttaac actggcaata taacaaaaca 27600agccataaaa cgaggcaaca gcagggtata agtgagcaat ggtctttgga ttaaatccgc 27660tatcatctta tgccttgtat cttcatcatg gtcatttctg caaaactgtg caattcatta 27720ccaagccacg aggcggtagc aaacagtgtg accaccacag cgatcaattt gataacgaag 27780cccagagttt gctcttggat ttgcgttaaa gcttgtacta aagataccaa agttcctacc 27840accgcagcca ctaacaccgg cggcattgaa aggactagca ccagccataa tgcctgactg 27900gtgaagtgaa ttatgtcacc ttgactcatg tcaccctccg tagctaatca ccagcccatg 27960cgtgagtcgt gtccagccat caagtaaaac aaatagcagc aatttaaatg gcagtgaaat 28020agtcatgggg gaaaccatca tcatccccat tgccaacaag atattggaaa taaccaggtc 28080aatgacgata aatggtaaat agatgagaaa gcctatctca aatgctcgag tcaactcact 28140caccgtaaac gcaggcaata aaatgaacag gctgtctgac tctaaccggt cagcatactg 28200cttgggccat aactgtttag tgctgtcaac aaaaaaagag tattcttgcg cttgaatatg 28260ctgcttaagg aacatgcgat agggggcaag cccttcatca aagaatttct caacagattc 28320tatgttcgtg aggctaacct cattagcttg taaatagtct tgcgtcgcga agccaacggg 28380tgccattaca taaagactaa ggatgattgc taagccatac attgccatgt tgggggggat 28440ttgctgtacc ccaagggcat tgcggagtag tgaaaagacc accgcaaatt tgacaaacga 28500tgtagccatt accgaaatca atggaagcag agtcagcaaa gataaaacga tgatgagatt 28560aatttcatcc ggtaactgga tcatgaaatc gtaacctctg tcaggcgttc aattcgaacc 28620ccgaggcgcc cttggatctc gaccaatcga ccatgtccaa gcaaccggcc gttagccagc 28680aagcgcactt caccatcaac aggtgttgta agatcaataa gagaacccgg ctccaggcta 28740gtgagtgtgt gccaatctaa gatttgccgc cccacttcaa agctaacttg aaccggaagt 28800tggttcaaat cggtcaatgg ttcggggtta agttcgtcag attcatgact cataccgata 28860aactccaatt tatttgattg taattgaaag tagccccaag ggttctcacc aacataggcg 28920agtactggcg aattaggccc actcccctca ggggccaaca acacatcacc taatcgaagg 28980gaatcaacct catctagagt caggtatact ttatgccaac gcaaagaaat aaggataggc 29040aaaggtatgc gctcagaatt gggtcgtgcg ggtaacagag cgaacagagc ctcagctgag 29100gttagccaga aggagatatg cgcattatct cgggacagcc gcaaactcaa cagaggttgc 29160gtgacagaaa gagacgctgt cgctatatca ttacagacca gtttcggcag gaaaactgtc 29220tggcgttcca acagcgcaag ttgcaactct ttcggcagag taaagaatgg agcccctagt 29280aagtcggcgg ttaaccagtt agccagatca ttgccaaaac agtagagcgt gaagtgcgtt 29340cccttccatt gtaactgtaa tatacagttc aaagacgaag gaggctcaga gacggtaagt 29400tcgagttttc cctcttccca caagtagttt tgttgataat ggctgagccg ttgacgtagc 29460gacagttcac ttaatttggc ctgtggcaag gttaacaaac tcattcttca gcctcccact 29520cctcatagac gtggcgtttc tgacgtgact cctgttcact gtcatcgcta gcttgaaaat 29580caagttgtgt tggctcaatg cgttgtaatc gctcaagaag atcataactt ccctgcgcta 29640aaattcttaa agcttcccga ctggcgatca gttctacatg cagttttccc ggaatctcag 29700caatacgaac cataatagcc cccaattcag gtaagttaag gtgtaattgg gttacttggg 29760atgagccgcc gcgcagttct agttctaccg ctagtcgctg tgctaactgt ataagttgct 29820ctgagactga tgccagcctg gtcgcgcgca ttgttgctaa taggtgatca cccggcgtga 29880caaccggtgc tgtaaaagac aaggaataca tctccggcaa ggtttcttcg cgcggaagta 29940acagtttttc tggtcgcggt ggttcgatgg tatctttgct gctatcaacg ccatcagtaa 30000gatgttggga ccgatccgcc agctcggtca tgtccagttg ctctgactgc actgtcgctt 30060tgtacggaaa acgagcagat ttttctgcta cctcagtcac aggagtagca caccatcttg 30120ctaccaaatg atcatccggt gtggcactaa ctgccggggg acatatctct ggcaatgctt 30180tttgatgaag agctaacggt tcttctgcaa ggcgtttttg cctgctatcg cgctggtttt 30240ttgctatact ggccggagtt tccctaccga cagaccatac aataacctct gcgccgtcag 30300cagccggatt caatggctga acagtcggct tgctattcaa ttgaactggt tgagcggaat 30360cgataggcga ttgctgaaca aataactcat catgtccaga cggctcgcag ggggattcac 30420ttgatgtccc cgtttcacaa gtcaccccct ctaaaaaggg cgccaatggc atgagagcat 30480taataatact ctctacgcgg tcatcaaaac ggctatcctg ctggtataag tgcgctctat 30540tggtagcacc ttcggcaagc ggagataagt tgaaatcatg attattctga tggttgtgtt 30600gaggttttaa tcctacctct ttccttccgg gttgagacgt cgccgctaat ggcgcatgcg 30660cacgcaagcc atctcctttc tgaccttctt ttttgccaag gtcatgcgga cgtacaggtt 30720taagcgactc ttctttggga tgacaattcc ccttattatt atgcaacagc gcttgctcaa 30780aatcgacaca tgcttgcaaa gcatgatgcg gcttccccag aggttgatac tcaggttcta 30840atggggaacg agtggtgatt ttattcatta ggcgttcctg tgatgctgta gaaactcttc 30900ttgttcttgt tcttcctgat aatgttgttg atttagctca tcttcatctt ctcgtcgcac 30960tagctcgaga aacttatttt ccttatgacg agcctgttgc agcatctttt ggcataacgt 31020aaggcgctca cgctcattgg ctaaacgttc caataatttg gcgcactcca gttcataatt 31080ggcctccttt tcacgtagcg acgctatttg tcgctgccat ttctccaaat ctttacaatt 31140cagtgtggtg ttttttcgct gatcaaatag acgttgctcc tcatcaatac gccacaaatg 31200gtaatcctga ctggtttgca ccgcctcttg atgacgtcga tgagctgcct gcaagcaagc 31260ttgctgagtc ttgatggctt tctccgcacg ttcaacgcgt aaaactttaa cccggtgcag 31320gcggcgtatc attgggtcag cgtctccaat aagttcagcg tctcattgaa atggcttaac 31380tcgtgcgtcc cctggcagag ccatcctcga atcgccccca tgcgttcaat cgcttgatcg 31440gcctctttgt cttgcccttt ctggtactcc ccgatttgca acagcaattc cacttcttca 31500tatttggcca ataaacggcg taagtccccc gcccaggttt tgtgctcctt gctgacaatt 31560tgattcatca ccctgctcgc tgaacgtaat acgtcaatgg caggataatg attagctgcc 31620gctaatttcc gtgacagaat aatatgacca tcaagtatcg aacgtgtttc gtcggccacg 31680ggttcggtca tatcgtcccc ttcgaccagt acggtataga gagccgtaat tgaccctttg 31740ctggactgac cagccctttc catcaaacgg ggtaaagcgg caaatactga cggaggataa 31800ccgcggcgag tcggtggttc tcccgcagct aagcctattt cacgctgagc acgagcaaac 31860cgtgttacag agtccataag taacaatacg cgtttccctt gatcgcgaaa atattcagca 31920atagatgtcg ccacgaatcc agctttggct ctttccattg agggccgatc cgaggtggcc 31980accacgagaa ccgctttgcg taacccctct tcgcctaaat cagactcaat aaactcacgc 32040acttcgcgtc cacgctcacc aataagcgcc agcacggtta cgtctacttc agcactacga 32100ataagcgaag caagcagtgt acttttcccc cccccggcgg ccgcgaagat gcccattctt 32160tgcccctcgc cacaggtaag caaaccgtca ataacccgga tccccaaaga aagtggtgtg 32220gtaataagtt ttcggctcat cggcgctggg gcatcctgat aaactgggta ccaagccgcc 32280ggttcaggga gatgcccccc atcgaaaggc tgccctaaac catccaacac ctgtcccagc 32340agatgttcac ccaccccaac ctgatgcatt gtccctgtcg ggctaacttc agtattagaa 32400gatatcccgt acatttcacc aagtggaata agtaatgctt gatgttgggc aaaacctatg 32460acttcagcct gtaaagacag gctgttgtca gggttacgta agtaacataa ctcaccgatg 32520cgcacacctg gcactaccgc ttttaataac gttcctgtca cttgagtgac acgtcctcta 32580atttggatta ggcggctacc tacaatgcca tgacgaatat gatgaggtat ctgatctagt 32640gagagcataa atccataatg gttgaaatat taaccactat tttagtgact aaaaacgcta 32700aaaaattgta gcgggagccg cgagttttta gaaaaatagc caagcagcac taaaatttct 32760cggctgattt tggcatcgat aagcaagaac tatttttata atcgcggtaa ttgtaattat 32820aaactgttca tctcagggag tagttatgac gacgcttcat aacctatctt atggcaatac 32880cccgctgcat aatgagcgtc cagagattgc cagtagtcag atcgtaaatc agactctggg 32940tcaatttcgg ggagaatctg tgcagatagt cagcggcact ctgcagtcta tagctgatat 33000ggcagaagag gtaacatttg tcttctccga gcgtaaggag ctctccctcg acaaacgcaa 33060attaagtgac agccaggctc gagttagcga cgttgaggag caggttaatc aataccttag 33120caaagttcca gagttggaac aaaaacagaa tgtgagtgag ctgctcagtc tgttgagtaa 33180cagccccaat ataagcttgt cccagttaaa ggcttatctg gaggggaaat cagaagaacc 33240gagtgagcaa ttcaaaatgc tctgcggctt gcgtgatgcc ctgaaagggc gccctgaatt 33300agcacatctt tcgcatttgg ttgaacaagc tctggtcagc atggctgaag agcaaggaga 33360aaccattgta ttgggtgcca ggataacccc ggaagcgtac agagaatccc agtcgggtgt 33420taatccactg cagccgctcc gtgataccta ccgcgatgca gtgatgggtt atcaaggaat 33480ttatgcgatc tggagtgatt tacaaaaacg ttttcctaat ggggatatag actcggtgat 33540attattcctg caaaaggcgc ttagtgcaga tctacaaagt caacaaagcg ggtctggacg 33600ggaaaaatta ggaatagtta ttagtgactt acagaagcta aaggagtttg gtagcgtgag 33660tgaccaagtt aaaggatttt ggcaattttt ttcagagggt aaaactaatg gcgtacgacc 33720tttctgagtt tatgggagat attgtcgcac tggttgacaa gcgctgggcg gggattcatg 33780acattgaaca tcttgccaac gccttttccc ttcctacgcc tgaaatcaaa gtgcgtttct 33840atcaagattt aaaaagaatg tttcgtcttt tccctctggg ggtatttagc gatgaggagc 33900aacggcaaaa tttattgcaa atgtgtcaaa atgcgatcga tatggctatt gagagtgaag 33960aggaagaatt gagtgagttg gattgaaccc atcatttccc atttctgcca ggatctggga 34020gtgccaacat ctagccccct ttcgcctctt attcaattag agatggctca atctggcacg 34080ctgcaactgg aacaacatgg tgcgacactg acattgtggt tagcgcgttc tcttgcctgg 34140caccggtgcg aagatgctat ggtcaaagcg ctaacgctca cggcggccca aaagagtggc 34200gctttaccgc tgcgagcggg gtggttaggg gaaagtcagc tggtgttatt tgtctcgctt 34260gatgagcgtt ccttaacctt gcccctttta catcaagctt tcgaacagtt actgcgattg 34320cagcaagagg tgcttgcgcc gtgagtcgca taataactgc cccccatatt ggcatcgaaa 34380aactgtcggc gattagcctg gaagagctat cctgtggctt gcctgaacgt tatgccttgc 34440cgcctgatgg gcatccagtc gaaccacatt tagagcgcct ttaccctaca gcacaaagca 34500agcgtagcct atgggacttt gcttctcccg gctatacatt tcatggatta catcgagctc 34560aagattatcg gcgcgaactg gataccttgc agtcactgct aaccaccagt cagtcctcag 34620agctacaagc tgccgcggcg ctgcttaaat gccaacaaga tgatgatcgg ttactgcaaa 34680taatccttaa cctgttgcac aaagtatgaa tattacttta accaaacgac aacaggagtt 34740cttgctgctc aacggttggt tacaactaca atgtggccat gcagagcgcg catgtattct 34800attggacgcc ttgctgacgt taaatcctga gcatttagcc ggtcggcgtt gccgattagt 34860cgcgctactt aataataacc agggagaacg tgccgaaaaa gaagcgcaat ggctaatatc 34920acatgaccct ttacaggctg ggaattggct ctgtttgagc cgcgcccaac aactgaacgg 34980cgatcttgat aaggctcgcc atgcttatca acattatttg gagttgaaag atcataatga 35040atccccatga tcttgagtgg ctaaatcgta ttggcgagcg taaagatatc atgctggcag 35100tgctgctgtt agctgtggta ttcatgatgg tcttaccact cccccccctt gtgttggaca 35160ttctgattgc tgttaacatg accatttcag tggtgttgtt aatgatagcg atctatatca 35220actctccttt acaattttca gctttccctg cggtgctact cgttaccacg ttatttcgtc 35280tcgcactttc agttagcacc acccgcatga tcctgctaca agctgatgcg gggcagattg 35340tttacacctt tggtaatttc gtcgttggcg gtaacctcat cgtcgggatt gtcatcttcc 35400tgatcatcac tattgtgcaa tttttagtga taacgaaagg ctcagaacgt gtagcagaag 35460ttagtgccag attctctctt gatgcgatgc cgggtaaaca gatgagtatc gatggcgata 35520tgcgagccgg ggtgatcgat gtcaatgaag cgcgtgagcg acgcgcgacg atagaaaaag 35580aaagccaaat gttcggttct atggacggcg ccatgaagtt cgtcaaaggg gatgcaatag 35640ccggcctcat tattatcttt gtcaatatat taggcggcgt caccattggt gttacccaaa 35700aaggattagc ggccgctgag gcactgcaac tctattccat cctcactgtc ggggatggga 35760tggtttctca ggtacctgcc ctgctgatag ctattaccgc gggtattatc gtcacccgcg 35820tctcttcaga agattcatca gatctgggta gcgatattgg caaacaggtt gtcgctcagc 35880ctaaagccat gctaattggt ggcgtactgc tgttgctctt tggtcttatc cccggcttcc 35940caacagtcac ctttctgatt ttggcgctat tggtaggctg tggtggttat atgctcagcc 36000gtaagcagag tcgtaatgac gaggctaatc aagacctgca atccatactg acgagcggtt 36060ctggcgcccc ggctgctcga accaaagcca aaacaagtgg ggcaaacaag ggccgactag 36120gggaacaaga agcatttgct atgacggttc ccttgctgat tgatgtagat tcaagccaac 36180aggaagcact ggaagcgata gcactaaatg atgagctggt tcgagtgcgc cgtgctcttt 36240atcttgatct tggcgtacct ttccctggga tccatctgcg ttttaatgag gggatgggtg 36300aaggcgaata tattatttcc ttgcaagaag ttccagtggc gcgaggtgag cttaaggcag 36360gttatttact cgtgcgtgaa tccgtcagcc aactcgaatt actgggtata ccctatgaaa 36420aaggggaaca tttgctaccc gatcaggaag ctttctgggt atcggttgaa tatgaggagc 36480gcctggaaaa gtctcaactg gaatttttct ctcattccca agttctaacc tggcatcttt 36540ctcatgtcct acgtgaatat gccgaagatt tcattggtat ccaagaaacc cgctatctgc 36600tcgaacagat ggaaggaggc tatggcgaat taattaaaga agtacaaaga atcgttccct 36660tacaacgaat gaccgaaata ttacaacgat tagttggaga agatatttct atccgtaata 36720tgcgatctat tcttgaagct atggtggaat ggggacaaaa agagaaagac gtcgttcaac 36780tcacagaata tatccgcagt agtctaaaac gttatatctg ctacaaatac gctaatggca 36840acaatatatt gccggcttat cttttcgatc aagaagtaga agaaaaaatt cgtagcggtg 36900tgcgccaaac cagtgcaggg agttatttgg cattggagcc tgctgttacc gagagtttac 36960ttgaacaagt tcgcaagact attggcgatc tatcgcaaat ccagagtaaa ccggtgctga 37020ttgtttctat ggatattcgt cgctatgtgc gcaaactgat tgagagcgaa tactatggct 37080tgccggtact ttcataccaa gagctgactc agcagattaa tatccaacca cttggacgaa 37140tttgcttatg atggcagatc ctttaattcc gtggcttacc gaacatggct tggtttgcca 37200ccctcatact ttgtctggca cccccatttc tttaggttcg gcctttcaat tagctggcct 37260caagcttgcc tggcgcgtag aaattgaaca aaggcgggtt tggatcgtgc ttatccaacg 37320agtggaacaa cgtcgagggc tgaaaaatcc cttcgcggca ctttatatgt tagctaatgc 37380agcgcgggcc gttcttggcc ctgactatta tctgtatggc aatgtcgatg tactggcggg 37440gagttctctc agtacgcaac ggctcgctca tttttatcgg cgttggaccg gggccaaaga 37500attaagcacc gggtggttct cactaaaagt atcacaagtc atcaccttat ctaatatgaa 37560aaagcgacaa aacaacggct ttgcctgaca agctaaataa aaataacgta atagaatagg 37620aggtagatta tgaagtcttc ccattttgat gaatatgaca aaacgcttaa acaggcagaa 37680ctggcaatag ccgacagcga tcaccgcgca aaattattgc aagaaatgtg tgctgatatc 37740ggcttaacgc ctgaagccgt aatgaagata tttgcgggcc gttccgccga agagataaag 37800ccagcggagc gcgagttgct tgatgaaatt aagcgtcaga gggagaggca gcctcaacat 37860ccctacgatg ggaagagacc aagaaaacca acgatgatgc gagggcaaat tatttaatat 37920gattagagcc tacgaacaaa acccacaaca ttttattgag gatctagaaa aagttagggt 37980ggaacaactt actggtcatg gttcttcagt tttagaagaa ttggttcagt tagtcaaaga 38040taaaaatata gatatttcca ttaaatatga tcccagaaaa gattcggagg tttttgccaa 38100tagagtaatt actgatgata tcgaattgct caagaaaatc ctagcttatt ttctacccga 38160ggatgccatt cttaaaggcg gtcattatga caaccaactg caaaatggca tcaagcgagt 38220aaaagagttc cttgaatcat cgccgaatac acaatgggaa ttgcgggcgt tcatggcagt 38280aatgcatttc tctttaaccg ccgatcgtat cgatgatgat attttgaaag tgattgttga 38340ttcaatgaat catcatggtg atgcccgtag caagttgcgt gaagaattag ctgagcttac 38400cgccgaatta aagatttatt cagttattca agccgaaatt aataagcatc tgtctagtag 38460tggcaccata aatatccatg ataaatccat taatctcatg gataaaaatt tatatggtta 38520tacagatgaa gagattttta aagccagcgc agagtacaaa attctcgaga aaatgcctca 38580aaccaccatt caggtggatg ggagcgagaa aaaaatagtc tcgataaagg actttcttgg 38640aagtgagaat aaaagaaccg gggcgttggg taatctgaaa aactcatact cttataataa 38700agataataat gaattatctc actttgccac cacctgctcg gataagtcca ggccgctcaa 38760cgacttggtt agccaaaaaa caactcagct gtctgatatt acatcacgtt ttaattcagc 38820tattgaagca ctgaaccgtt tcattcagaa atatgattca gtgatgcaac gtctgctaga 38880tgacacgtct ggtaaatgac acgaggtaat tatgcaacaa gagacgacag acactcaaga 38940ataccagctg gcaatggaat ccttcctaaa aggaggggga actatcgcca tgctcaacga 39000aatttcaagt gacactttag agcaactcta ctctcttgca tttaaccaat accagtcagg 39060aaaatacgag gatgctcaca aggtctttca agctctctgt gtgctagacc actatgattc 39120acgtttcttt ttagggctag gcgcttgtcg tcaagccatg gggcaatacg acttagcgat 39180tcatagctac agctatggcg ccataatgga tataaaagaa cctcgttttc cgtttcatgc 39240ggccgaatgt ttactgcaaa agggagagct tgctgaagca gaaagtggct tgttcttggc 39300tcaagagctt atcgcagaca aaactgagtt taaggagctt tccacccgag ttagctcaat 39360gttagaagca attaaattga aaaaggagat ggaacatgag tgcgttgata acccatgacc 39420gctcaacgcc agtaactgga agtctacttc cctacgtcga gacaccagcg cccgcccccc 39480ttcagaccca acaagtcgcg ggagaactga aggataaaaa tggcggggtg agttctcagg 39540gcgtacagct ccctgcacca ctagcagtgg ttgccagcca agttactgaa ggacaacagc 39600aagaagtcac taaattattg gagtcggtca cccgcggcgc ggcaggatct caactgatat 39660caaattatgt ttcagtgcta acgaagttta cgcttgcttc acctgataca tttgagattg 39720agttaggtaa gctagtttct aatttagaag aagtacgcaa agacataaaa atcgctgata 39780ttcagcgtct tcatgaacaa aacatgaaga aaattgaaga gaatcaagag aaaatcaaag 39840aaacagaaga gaatgccaag caagtcaaga aatccggcat cgcatcaaag atttttggct 39900ggctcagcgc catagcctca gtgattgtcg gtgccatcat ggtggcctca ggggtaggag 39960ccgttgccgg tgcaatgatg gttgcctcag gcgtaattgg gatggcgaat atggcagtga 40020aacaagcggc ggaagatggc ctgatatccc aagaggcaat gaaaatatta gggccgatac 40080tcactgcgat tgaagtcgca ttgactgtag tttcaaccgt aatgaccttt ggcggttcgg 40140cactaaaatg cctggctaat attggcgcaa aactcggtgc taacaccgca agtcttgtgg 40200ctaaaggagc cgagttttcg gccaaagttg cccaaatttc gacaggcata tcaaacactg 40260tcgggagtgc agtgactaaa ttagggggca gttttgctgg tttaacaatg agccatgcaa 40320tccgtacagg atcacaggca acacaagtcg ccgttggtgt gggcagcgga ataactcaga 40380ccatcaataa taaaaagcaa gctgatttac aacataataa cgctgatttg gccttgaaca 40440aggcagacat ggcagcgtta caaagtatta ttgaccgact caaagaagag ttatcccatt 40500tgtcagagtc acatcaacaa gtgatggaac tgattttcca gatgattaat gcaaaaggtg 40560acatgctgca taatttggcc ggcagacccc atactgttta agtttaagga ggaataacca 40620tgacaataaa tatcaagaca gacagcccaa ttatcacgac cggttcacag cttgatgcca 40680tcactacaga gacagtcaag caaagcggtg agattaaaaa aacagaagac acccgtcatg 40740aagcacaagc aataaagagt agcgaggcaa gcttatctcg gtcacaggtg ccagaattga 40800tcaaaccgag ccagggaatc aatgttgcat tactgagtaa aagccagggt gatcttaatg 40860gtactttaag tatcttgttg ttgctgttgg aactggcacg taaagcgcga gaaatgggtt 40920tgcaacaaag ggatatagaa aataaagcta ctattactgc ccaaaaggag caggtagcgg 40980agatggtcag cggtgcaaaa ctgatgatcg ccatggcggt ggtgtctggc atcatggctg 41040ctacttctac ggttgctagt gctttttcta tagcgaaaga ggtgaaaata gttaaacagg 41100aacaaattct aaacagtaat attgctggcc gcgaacaact tattgataca aaaatgcagc 41160aaatgagtaa cattggtgat aaagcggtaa gcagagagga tatcgggaga atatggaaac 41220cagagcaggt agcggatcaa aataagctgg cattattgga taaagaattc agaatgaccg 41280actcaaaagc caatgcgttt aatgccgcaa cgcagccgtt aggacaaatg gcaaacagtg 41340cgattcaagt tcatcaaggg tattctcaag ccgaggtcaa agagaaagaa gtcaatgcaa 41400gtattgctgc caacgagaag caaaaagccg aagaggcgat gaactataat gataacttta 41460tgaaagatgt cctgcgcttg attgaacaat atgttagcag tcatactcac gccatgaaag 41520ccgcttttgg tgttgtctga ccattgatga ccttggttag ttaattaacc gaaagtttta 41580ttttacctta ccccttatgg tgatagagct tatctatata aggtataagg tgctgaaaag 41640ccctgtatta acattagtta atccagggtt gtgattatta aattaaaaat aataagttag 41700gatcatatga caattaaaat aaaagattat ttacatgtag tagctcaaga cctgagctga 41760cagttaccgg ttgttgaacg gcaatacgcg gtcattgagc acgtcagcgg ctgtgatcgg 41820cattttgctc gtatacagcg agagtgttag aaatgctgtg tcattccagt aatatgcaat 41880caaaaaagaa tgacacatat cccaataatg agagtcggtg attttactca ttgatggggg 41940ggaataatta ggctaaaaca acctcaatgt taaagagccg aggtgttcgg taagctctgc 42000atttaacgct gtttcgacgg taagctttgt tagcatacga gaaaatgcat taaggtcggc 42060ttcggtttta agacctttag ccagttcagc cgcaagtgct ttaagtttct tttcgtccat 42120aatttgcctg tctccgttgt tggagtgaag atatcaaaaa caggcaatta cacaatattg 42180tttacagtct cgattcataa aggtagatcc ttcccgcact caatattcag gttcgtcacg 42240gcgtaaccaa atatcaaatt gacctttatt cagtcgttgc aatgtttcaa atccctgaag 42300cgttgaccag gcacggtttg gccgtttgaa atccccggcc gcgtttacca attttttgat 42360gggggcatgg tcagactcga tacgattatt caggtatttg acttgccgct gctttgcagc 42420atcccgtatc ttttccttct ttcatcaaac gagtgatagc gtaaccgtat gacgaatgtt 42480tatcggtatt gagtatttta ggctgtcttt caacagaata gggttttaac acccgtttaa 42540tgaatggata ggcggtattt ttatttcgtt taggcgaaaa ataaaaatct aatgtagtgc 42600cgtgcttatt gatggcgcga tagagataaa accattttcc gttgaccctg atatagattt 42660catcgagttg ccatgaggag tcggcatccg taaattgata tcgtttcaat ttctgacgag 42720gtataggtgc atattcaata aactaacggt aaatggtaga gcgataaacg gaaatcccac 42780gctctgacag catatcgctg acattggcat aactcatcgg gggcaaaatg ttcccacttg 42840aaatcacctg gggccatgtg gattcactga agaagggatg aatagcctca gttttcatga 42900caactccatt ttttgcaaca agcccaaata tagtgatgct gaagtggcac aaacgctgga 42960taccgcgcaa aagcacaccc tgacggtttc aaaaggggta ctggatacgg ccaaacagta 43020tactaacacc gtcagcagta atacattgga cagtgccaat acctatacca ataataaagc 43080agactaacat tgaaggatgc taataattat actgttcaga aggtaaacag agatgctata 43140agtggtgtta tagatcagga attatattga gaaaccattg aagaaactaa agagcaaatg 43200gccggtattg aaactactgc cccaaaatac actgatttaa agtttaatga tatttccggt 43260aaggttgact ctgcggccat acaatacttc tgacatattt cttctggtta tttatgcata 43320aaaatggcca aaaactttca atggtagaag agctaaattc ggataaataa cgcataaaaa 43380ttcccggcga aaaactatat acatatataa atttaatatg tatgtttttg tttgcagtga 43440aaaactcgat aataaaaata ttttcagaaa ggcattcaat atgttcataa atccaagaaa 43500tgtatctaat acttttttgc aagaaccatt acgtcattct tctaatttaa ctgagatgcc 43560ggttgaggca gaaaatgtta aatctaagac tgaatattat aatgcatggt cggaatggga 43620acgaaatgcc cctccgggga atggtgaaca gagggaaatg gcggtttcaa ggttacgaga 43680ttgcctggac cgacaagccc atgagctaga actaaataat ctggggctga gttctttgcc 43740ggaattacct ccgcatttag agagtttagt ggcgtcatgt aattctctta cagaattacc 43800ggaattaccg cagagcctga aatcacttct agttgataat aacaatctga aggcattatc 43860cgatttacca cctttactgg aatatttagg tgtctctaat aatcagctgg aaaaattgcc 43920agagttgcaa aactcgtcct tcttgaaaat tattgatgtt gataacaatt cactgaaaaa 43980actacctgat ttacctcctt cactggagtt tattgctgct ggtaataatc agctggaaga 44040attgccagag ttgcaaaact tgcccttctt gactgcgatt tatgctgata acaattcact 44100gaaaaaacta cctgatttac ctctttcact ggaatctatt gttgctggta ataatattct 44160ggaagaattg ccagagttgc aaaacttgcc cttcttgact acgatttatg ctgataacaa 44220tttactgaaa acattacccg atttaccccc ttccctggaa gcacttaatg tcagagataa 44280ttatttaact gatctgccag aattaccgca gagtttaacc ttcttagatg tttctgaaaa 44340tattttttct ggattatcgg aattgccacc aaacttgtat tatctcaatg catccagcaa 44400tgaaataaga tccttatgcg atttaccccc ttcactggaa gaacttaatg tcagtaataa 44460taagttgatc gaactgccag cgttacctcc acgcttagaa cgtttaatcg cttcatttaa 44520tcatcttgct gaagtacctg aattgccgca aaacctgaaa cagctccacg tagagtacaa 44580ccctctgaga gagtttcccg atatacctga gtcagtggaa gatcttcgga tgaactctga 44640acgtgtagtt gatccatatg aatttgctca tgagactaca gacaaacttg aagatgatgt 44700atttgagtag tacgcaagag cgttcataat tctgcgtcac gttaaaatat cattacaacg 44760taatcacttt atcgaggcga ccttcaaaat aaatcgccaa ctgtgacaat gccaaattcc 44820agctctggat tggcattgtc catctttcct gcgcattcat taatcccaga tacagtgatt 44880tcaacagact gttctcatta gggaaaatgc ctttcgtttt tgtcagcttt ctgaacagcc 44940gatgtaccga ttcaatggca tttgtcatgt aaatgacctt gcggatcgtt gtcggatacc 45000ggaagtaaca cgacaaattg gcccattttt tccgccacga ctggagtacc actggatatt 45060gttggcccca ttaccagttg aggctcaaaa gttccattgc gatcacgggg cgtggcgagt 45120tcaaacgtac cagtgggagt tttgaccgtt tttctggacg aaccattttt gcggttggct 45180tcgatgtcca gagccagatg agtctgacca tgcccccatc aaaaaattgg taaacgcggc 45240cggggatttc aaacggccaa accgtgcctg gtcaacgctt cagggatttg aaacattgcg 45300acgactgaat aaaggtcaat ttgatatttg gttacgccgt gacgaacctg aatatcgagt 45360gcgggaagga tctaccttta tgaatcggct ctttaacatt gaggttgttt tagcctaatt 45420attccccccc atcaatgagt aaaataccga ctctcattat ttgcagcaaa cctatatatt 45480gcgctgaatc atagatcacc catcataact ggggataaaa ccacttaata tgttgtaacc 45540ccacggtttc atatgagtat tctccgatta catatatgtt agttgccatg gataatccgt 45600aatgttaaat agagttattt tctgtgtcac aatcataaat aacacaattg ttctttcaga 45660actcaacatt ttaatatact gtagcaatgg tatcaatagt catacttatt aaaatgatat 45720tttcctcaca gttaattaca cagcgtgaag taatgatatg gtgtttattt tgttattaaa 45780tgtcgtatct tgtttaatgg ttttatttat gtgtttaact gatttcaatg taaaaaatat 45840tttttacatt tagtaagtca tgtcaatgat atttgataaa gataattact gcttaattga 45900tggatatttt ttgttttttt aatcaacggt ggcgtcgcta tcgtcttaac gttcacagaa 45960gatatcattc tgcatagctc cgccagcatc caggcaagca tctactgtca gaaagtgctc 46020aactttcata ccagcataaa aagcagcgca caatgcggtg gctatcacta gtcgtttttt 46080cattgcatag tcctcgtagg ttagattatg ccccatgagc tgagaagagt gagctatcgt 46140tgtcttgagc tttctgtccg gggtatcatc gaattttact ttatttacat agaatttata 46200ttctatcata ccataattaa actttacttg aataaaaaca tcggtgtgct gaccatattc 46260ctgatatgaa tataataata tagccttaat gctcatggta ataaagtgcc ctacagcaat 46320ttcggcgggt ttgctgcaaa taatgagagt cgtgatttta ctcattgatg gggggggaat 46380aattaggcta aaacaacctc aatgttaaag agccgattca taaaggtaga tccttcccgc 46440actcgatatt taggttcgtc acggcgtaac caaatatcaa attgaccttt attcagtcgt 46500cgcaatgttt aaaatccctg aagcgttgac caggcacggt ttggccgttt gaaatccccg 46560gccgcgttta ccaatttttt gatgggggca tggtcagact caatacgatt attcaggtat 46620ttgacttgcc gctgctttgc agcatcccgt atcttttcct tctttcagtt ttcatgacaa 46680ctccattttt tgcaacaagc ccatagtggg gacaatgaac attaacgccg atcatgagaa 46740aaacttaaaa gtgagcatta tatataaaat tcaactaatt ggaggaatca ccgaaatact 46800taatggtggg gttattaact gggggatatt taacttggta ggatatttca aatcgtctat 46860atcactaata aaaataataa ttattgataa cactaatttg gtcatgttat atgtaaaaat 46920ttggataaat aatgaaaact tcttaattta tagtgaatta aaaacaaatg agttattata 46980taaaccatat ctattaaatt taatagatat tattgtaact atgtagtgaa ataactttgt 47040atggtaccgc gtatatgatt gtttacattt cagatgaata atatgggtga tgtcgagttg 47100ggctgaaact tagtattttg cggttctttt ctctgctcaa tatcatcaat gaaacgttct 47160aaccaagcct gcatttcatc gatatccaac cctaccagtg attgttgaga ccagagaata 47220ggtttaccgt ccagtcttcc ttgaacatgg gcaggccaat gttcgccaaa caaattatcg 47280ttggttggca aatcagcacc tagctcactg aacaactgta cccattgttg atgataacaa 47340gcaatcagta cttggatgtg cccatccact tcaaatgtaa atgtataaac gtcattttca 47400ttaacttgat ggttcaagcc aagcttcagg aaaagctgtt gcataagttc tgtgaaggtt 47460gtctgcatta aacctccttg gagtcaaatg ttaacactct aaaacgctgc cccaccccca 47520gaggataatg atacatagaa ttaccccaga atgagttagt aaaccatttg cggaactttt 47580ccttatcaga aaagtggaat tcaccgaaat tgggatcgaa gaaagtaaca ccactctttt 47640cgttgacata cgccgctatg gcgtgggctg acatttggcc tgagagatgt atttttttat 47700aaccgtaacc tatcccatga gtatcaagga tagcgtttaa taattgatcc agcccttctg 47760attccgtcgt accagtaaca tcaactggac gcagtaagca atgccgttca atcatacgtt 47820ctgatatgcc atttttcttg aaccaatcta gtgttacctc atcttgatca acgtctgctt 47880tacaaccatc tatttgcaac tgtttaattg agtaaagtgt atcgatctgg aatttcccct 47940tacgcccgcc aacatagagc tggtcaaata agctttggcc ttgtgcatgg ctcctgatcc 48000aatgtgcaca taaagcctca cagacaccgc tagcagtgtc tgaatgtttt attattttat 48060gaagaaaagc ccctttggtc tgagcaaact tgaaattgat gttacctccg taatttgcaa 48120cagactctct caccgcgggc acacgagtgc tgagtacaaa atgtatcatg cgatacataa 48180tcatactgaa ggtaaagctg ctgcgttggt tagctttgcc gtcgctggtc aactttctat 48240ccagcataga acggcctgac tggttatgtt ttatggtggc tgataacttt ttctgcaggt 48300ttgagtgtga cagtgctgtt tccactttca ctcggtgtgc gccaatcacc ccttcggtga 48360gggtagctga ttgaaggttt tcaccggcag aataattcga tagttgaata tggtagtgtc 48420cgtgaatact gttcatctgt ataacctatt tatgttagcc attattttgc tataccgata 48480aattgaatat atctggattt tgacgtctgg caatgaacga tagagcctac aataaattat 48540aaccaatagg tgactcaggg attttctctg aatcagagta catgttgtac attcgattaa 48600atattttttc aatagttaaa agttactttt tattataaaa attcaactta tggggacagt 48660gatgttatgt tgatagcgtc ggggcgtcgc ggggagagtt tataataaac tctaatgtga 48720taaaaatcca ttctaataat gatatattat actatatctg tagctttaaa ataaataatt 48780atagagtgga ggatgcttga aatatttcga tgcatgggaa gctcattgag agtagatatt 48840ctatttttat aaattacgga gtgattttaa ttatacactc gtagtgacgg ttattaaata 48900gtgtagttta taaagtaaat ttgggagtag taactatgtt tattaaagat acttataaca 48960tgcgtgcttt atgtaccgct cttgaacagt cggctcctga tacaataata aatacatcta 49020aagaagaaaa taacagttac tactgcgcta ctgctcattt actgagaacg gatgtttgtt 49080cattggtcaa tagagtaggg attgaaccac ttaaaagtgg atcaatatta tctactttag 49140aagagttatg gcaggctgtt ggtatagtat atcgcttata cgaatggcaa catgtcagcg 49200atattgacac caattttaag aaactaccca ataattctga ttttggtctt gtgttttctg 49260tattagattg tgatatagag tatgtgttca tagggaaaaa agacagtgaa gggaatatag 49320aattttatga tccgaaaaac tctctactta tagagaatga tgacataaaa aaatatttat 49380atgatgaaga ttttcatcgt ttttgtatta tgctgatcat ctctaaatct gagttggagg 49440aattgagtcg cgaatcctgc gatcaagaat gtattatggg atgaagctat attaaagagt 49500ttgggatatg gtagttgatt atgttaaagg ttaattatct gtaacatata aaaaacagtg 49560gtatgtaatc atcctgcata atcgtaccat tcatatttag agatcttccg gcatactgac 49620cttgccaatg aaggagatcg ctaaacgggt acaccgtatc tattgcctcc tgaaactcaa 49680tattcgccgc aaagggaaac aacgcctgcc agcctgtaac ccatcaccgc tggcggtacc 49740ggaacgactt aacctgagcg ggtcggtcga ttttatgcac aatgcacaat gcacaatgca 49800ctgagcggtg ggtgtcattt cagtacgtta taatgtcatg gatgattaca atcgtgaagc 49860actggcgatt gtaatcgatc tgaacctgcc aacacagcgc cgtcatcaga gtactggatc 49920gcattgtggt caaccgtggc tatgggaggt gccatgccct gttttaaatg gaagatgata 49980tgaagaaaaa catgaagtta atagcaatga ctgccgtact gtcctcagta ttagtcctct 50040ccggctgtgg tgcgatgagc acagcaatca aaaaaacgta atctggaagt gaaaacgcag 50100atgagtgaaa cgatctggtt agagccgtct tcacagaaaa ccgtttatct acagataaaa 50160aatatctcag ataaaaatat gcttggctta gccccccaaa tcacaaaagc tgtgcaggat 50220aaggggtata ccgtaacatc ttccccagaa gatgcacatt actggatcca ggctaatgtc 50280ctgaaagccg ataaaatgga tttgcgtgaa gctgaaggat ttctgagtca ggggtatcag 50340ggtgctgcgc tgggggccgc attaggggct ggtattacag gctacaactc taactcagcg 50400ggagccacgt taggaattgg attggcggct ggtcttgttg ggatggccgc gaatgcgatg 50460gtcgaggaca tcaattatac tatggtgacg gatgtccaga tttccgagaa aacggacacc 50520accctacaga ctgacaatgt ggcggcgctg aagcaaggca cctctggcta taaagttcag 50580accagcacac agacgggcaa caaacatcaa taccagactc gcgtggtttc ttcggctaac 50640aaggtcaacc tgaaatttga agaagcccgg ccggttctgg aagaccagct agcgaagtct 50700atcgctaata tcctgtaagc cataagcatc ctggtatgaa gatgtactgg gatgtagtgg 50760atcagtaata ctggacacct caatagcctg ttaatttaat gacagccaat tgaggtaatt 50820gataatgact caacctaaac agaccaaacg ccgtttttct cctgaattca aactggaagc 50880tattgagcag gtcgttaagt atcagcggtc aaccatcgag gttgcacgcg ctctggagct 50940ggatcccagc caattgcgta aatggatacg ccagtacaaa gaagaagtca gcgggatgac 51000gccggacaat cctgcactga caccagagca acgtgaaatc cagtcgctca gggcgcagat 51060taaacggctg gaaatggaaa aagaaatact aaagcaggca gctgtgttga tgagcgagtt 51120ccccatcaaa tctttgcgtt aacacggctg aaaacaaaat ggccagtggt cgaattgtgc 51180cgcctgctca aaataacgcg cagtgtttac tctgcttcgc tgaattttcg ggttgatgta 51240aaacgtctgc aactgcgtga attgcatcaa cagagccggg gagcagccgg cagcagaaca 51300ctgagtctgc tgatgcgtca gtcgggttat aacgtggtgc gctggctggc ccgcaggctg 51360atgcgggaat gtggtctggc gagtcgccaa cccggaaaac ctcgttaccg tggcgaacgg 51420gaggtgtcac tggcatcgcc agatttactg aaaaggcagt ttaagccgtc ggagcccaat 51480cgtgtgtgga gtggatatat cagctatatc aaagtcaatg gtggctggtg ctacctggca 51540ctggtgattg acctttactc ccgtcggata gtgggcagtg ccatatcgtc atccccggat 51600gctgagctgg tgtgtcgagc ctagcgtaat gcactggaga cgcgcccaag ggaaaagagg 51660ctgctgtttc attcggatca gggagggcag tacaggagta agaaatccag gcagttactg 51720tggaggaccg gagtgatgca gagtatgagc cgcaggggta actgcctgga taactcacca 51780atggaaagag tgttccgaag cctgaaaagt gaatggctgc ttgtaggggg ctatatggat 51840gtccatcatg cggtacgaga tatcggtgaa tggatacaaa gttattacaa caccccccca 51900tcggcacaat ggtggattac cgccctgtga atacgaagag cggtggaaaa aggctacgaa 51960ggtgtcctga ttttgtgatc cactacacta cattcaggag agttggacca gaaatcaggt 52020aataaggtcc ggtccactcg ccttcaaatt caacatgtaa ttcattgtca aacagtcccc 52080agagcagtaa aacagagatc cggttcaacc tcatacgccg tgccctcggc catgatgcgg 52140gcaatcgata cccactttgc ggcgttcagg ctcttgggca aagcgacaga actgctccca 52200gttgcacgta tcgcggatcc cctcggggga acatttgtca gccagtcttc ctggctagaa 52260tgtgtttaga ttatccgtgg tgattcagtg gttgcacggt atcaagcaaa aaccaaggac 52320actcttagtt tgaaaaggca ggtaatatac agcttcagtt aacccatttt actgactgat 52380atttatcgtt ttttgtagca agacgagtgt tttcgattat gtacgtatag gataaatttt 52440tagtacctat atattggcat gccctgctgt taccgacgag caaaaagaaa ggtcatctac 52500agcagattag gtaactgtca aacttggggt atttcccata gtcgtcatat agacggaaat 52560gtgaacgtgc ccagttcggc attgaggctc ttacgcttca tcgcgatgct tggtcactgg 52620ctgcggcgaa cgttgatatt ctatttatat atctttgcgt tatttgggtt gttttgcgta 52680ttttttgatg ttttactata aagacgcaaa tttcatagag ataatacata tggacctaaa 52740gtcaactctt gaccgctgta ttgaacgtgg acagttcatg actcaagaaa ttgctaaatc 52800acaattcggt aatgacagtc cggctgctcg aacgattact agacgctggc gtattactga 52860agctgctgaa cttgtcggag taacaccaca aacgatccgt aactatgaag actcaggcaa 52920actgccaccg cctgatacag caatgattgg tcgtgttgag caacgaactg gatattccat 52980ccagcaaatt aatgatatgc gtgatgtgtt taaaacaaga ctatccaaac caaaaggcga 53040aaatcctgtt gttcttgcca ttgcagctca taaaggtggt gcatacaaga catcgacatc 53100tgttcatatt gctcaatgga tggcgttaca agggttacgg gttttgttga ttgatgcgac 53160tgatcctcaa gctacggcct ctttatatca tggctatgtt cccgatctgc atatacatga 53220agaagatact ttgttgcctt attatcttgg tcaacgagat gatgctgctt atgcgataaa 53280accgacttgc tggccaaatc ttgaagtcat tccttcttgt ctggcagtgc atcgtattga 53340gtcggaaatt tatggcttgc atgatcaggg gaaattacct gtagcccctc atcttttatt 53400acgtgctgct attgagtcag tctgggatag ttatgatgtt gtggtgttag atagtgcacc 53460aaacttaggt attgggacta ttaatgtcgt gtgcgctgct gatgtcatcg tagtgcctac 53520tccggcggag ctttatgact atgtttccac gttgcaattt ttcaccatgc ttagggattt 53580gatgtcaaat attgatctca acggttttga acctgatgtg cgcgttttaa ttactaaatt 53640tagtaatgcg atcggtagtc agtctcagtg gatggacgat cagataagga atgcatgggg 53700tggaatggtg ttgaaagaag ttgttcgcgt gactgacgaa gtggggaaag gccaagtacg 53760aatgcgtact gtatttgaac aggcagctaa ccagcgttca acgccagctg cttggcgtaa 53820cgctgtttcg atttgggagc ctgtttgtgc agaaatattc aatcggctgg ttaagcctcg 53880ttgggagaat gcatgatgaa aaggtcacca gtgttacgta atgcgccttc aattaatttt 53940gatgatgcta aacccgcaat cagcaatgca gagccctcgg tttctgctcc ggcggtgagt 54000cagcttgctt ctcgagttag tggcatgaaa ggcaacacaa tcgtattacc tgtttgtgga 54060aggaacgttg cttttacgct taaagtgata gcagcacctg atgttgaatc taaaacaatc 54120gtttttagtg gtaatgagcg aaaccaagca ttattaagtg agacgtcgtt agatgacttg 54180atcccttcat ttttaacgtc agggcagcag atccccgcct ttgcacgtga acataacggg 54240aacatcgagg ttgctgatgg aagtcgccga cgtaaagccg cgatactcac cggaagtgac 54300tataaggttc tggttggtaa cttgaatgat gagcagatgc tatggctgtc ccaaattgct 54360aatgagtatc gtccgacgag tgcttatgaa cgaggcctgc gttacgccca acggctaata 54420tctgaatttg aaggtaatat tagtaaattg gcagaggccg agcatctctc tcgcaaaatt 54480attcagcgtt gtattaaaac ggctgggctg ccccttaaaa ctattcaact gtttgctaac 54540cctaatgaat taagtgctcg tagtggcgaa gcattaagta aagcttatga aaataatgtt 54600gatacgctaa agcgagttac gcataaaatt atgaagcaaa aacaggaagg tcgccagttt 54660actacggaag aattaatcgt attgctgatg cctgagagaa aacagccaga gaacattcat 54720aaaaaaagct ttggcaaaaa tatagaagca aaatattcaa aagacaatgt ttctttctat 54780ctcaaatctg tgccagagtc cttggttaaa caaatagaag aactcttgaa tacctatgca 54840aaggaacatt ctttgtagtg cctgaataag atcagaacaa ggtgggttgt ctgcccgcct 54900ttatttagtg tatatctatt gttttagctc atccttcgta gaagatctcc ttctttacaa 54960ctcatttcct aagctgaact gtggccccaa tcaccaacgt tagcattgga tatccaggtg 55020ggaccgtggt cccaattact aacgttggca ttggatatcc aggtgggacc gtggtcccaa 55080ttaccaacgt tggcattgga tatccaggtg ggaccgtggt cccaattacc aacgttgaca 55140ttggatatcc aggtgggacc gtggtcccaa ttaccaacgt tggcattgga tatccaagtg 55200ggaccgtggt cccaattact aacactggca ttgaacatcc tagtggaaat gtgattcgaa 55260ttgaaagcga ttaagtcgat gagcattttt ttagtgatag ctttagggag attaaactag 55320cggatatttc attatggaga taataatttt cgcttcattt atcacccctt cgcaatactc 55380tgttcaccaa gtcatactat tcttagccca gagctattga tgcatcaaca acttgcatca 55440gatgtttact gttcgcgtgc ggcggtgttt catcaaacat atctatctga cctggtcgac 55500tgccgacgtc attaagcatg atgtcggctt tttgatagcg atatccatcc cgccaggttg 55560gatacggcga tttcatgtta gtatcgatgt aacaatgacg gctctattgc attaaggcac 55620agtctggtaa catgctgttt tttttatgat attgcctgca catatcttta atcaaaaaat 55680ttttttgtcg cctgcattac cccatcgatg ttatagctca gtgtgttcac tggtatcttg 55740gcgatgccct gagttttcta aatctagagg aaatgatgac gaaacacggt atctctgttg 55800agcattacac actccacagt tgggttattc gtgtggtgcc attactgcat aaagcttttt 55860gccgttataa atgtaccgtg ggtcgccggt ggcgaatgga tgaagcctca aaggtcagtg 55920gaaatacctg taccgggcgg ttgatactcg cggttagact atcgattttc tgctgacggt 55980aaagcaggat gcggcggcag cactgtgccg actggtatca ggtctcccgg taaattcata 56040tattaaagta ggcagggctg gctcattaca gattgtttag ctataattgt attactcagt 56100aatacataac ggaggggata tggctcacgt aaccagcgta acacttggag agcatttgac 56160aggttttgtg ggggaaatga ttcagtctgg tcgttatggc aatatatcag aagtgcttcg 56220tgatgcctta aggctaatgg aagcacgtga acagcgtgtt caacatgtac gcgatatggt 56280gcttgcagga acaaatgtac ctgtgagcca tcgtttaatg gatgagattt tttctgctgc 56340ggtgaaagat actagtgtat aaactgtctg aactagctga tgaggatatt tataatattg 56400ccagttatac tatccggcat ttcggtgtga ctcaggctaa gttataccat gagaacttgg 56460caaaggtatt cgagctatta gctaaaaatc cagagttagg ggctgagtgt aactggattt 56520gctctgatat tcgccgtttt cagtataaaa agcacggtat atactatata acgcttagca 56580atgatatttt gatttctcga gtgctgcatc aatccataga tatagatgtt caggattttc 56640cagagcatga gtagtaatac agaagagaga taagtcagaa attctaacaa tgagcatgct 56700aaaaaacgat tcgcccctga aagatcaggg acgaagatat tcgcaatatt gatagaaaaa 56760gggggaaaca ctatcccctt gtttttatcc atatcacatc aatgacagta atttctgcat 56820ctgttgcgcc agccctttga tctcatttgc tgcctgcgtt agatcaacgc cactggcgac 56880gtacgcgctg gccgccccac caatagttcc ccactgcgag aagggaatac cacaaacagg 56940cgtgtttaag atggcactgg cctcagcttg caattcccca ccacaaaact gcatcagccc 57000ttggcattga gtgatactgc cacgaagagg gccgctgccc gtagcgaact gatcatgatt 57060tttctgcagc atctctgcat ccaagctatt caactgctgc atgtattttg gcagcgtctc 57120agcagcaagt tgcttgatac tgtcactgaa agacgtagga cttggcattt gtgcaggtgt 57180gggtgctggt gtcaccaccg gtttatggct cccctccgag aacatgcgtt ggataaaccc 57240aatcacagag tgggccactg atgataacct ctcaatgata cggctggcta agctggaacc 57300ctgagggctt tcagtgcgcc cggccagatt gtttgcatat tgatcacttg tttgctgtga 57360gactgagcgc ccagacattt ctcctacgct gctagatcct gacacagatg tcggcagggg 57420cagtgatgta gaaataaatg atgatatttt catgactatt tattaccttg gctattaaaa 57480caaggttatc ttagtgggaa aatagccgat ggctatataa aaaatcgctg ctgtttttgt 57540tgttatatta gacaaaacaa aaactaaaaa ttataggcta aaattgatgg tctgccgaga 57600gtgctttggt taagttgata ttttatctaa ctattatgag atcataatgt attcatttga 57660acaagctatc actcaattat ttcaacaact ttcgttgtct attccagata ctattgaacc 57720ggttatcggt gtcaaagttg gggaattcgc ctgccatata acagagcatc ctgtcgggca 57780aatattaatg tttaccctac cttctcttga caataatgat gaaaaggaaa ccttacttag 57840ccataatata ttcagtcaag atatattaaa acccatctta tcctgggacg aggttggggg 57900gcacccagtg ttatggaatc gacaaccatt gaacagcctg gataataact cactatatac 57960tcagcttgag atgctggtgc agggggctga acggctacaa acctcatcac taatctcacc 58020accacggtca tttagttgag tagatttttg gttgttgcct tattatacgg aatgacctgc 58080ccccaggatt agatacaacg ctcacttagt aatgtcggat ccttcactat cagaattacc 58140ctttctccag gccgccgcaa attcagacgg cgtctgataa ttcagcgtag agtgcgggcg 58200gcactcatta taatcctgac gccattcact gatggttttc ctggcatgac tgacgtcact 58260gaaccagtgc tcattcaggc attcatcgcg aaagcgtccg ttaaaactct caataaatcc 58320gttctgtgtc ggcttgccgg gctggataag tcgcagttcc acgccatgct caaaggccca 58380ttgatcgagc gcgcggcagg taaattccgg gccctgatca gttcttatcg tcgccggata 58440gccgcgaaac agcgcaatgc tgtccagaat acgcgtgacc tgcacgcctg aaatcccaaa 58500ggcaacagtg accgtcaggc attccttcgt gtagtcgtcc acgcaggtaa ggcactttat 58560cctgcgaccg gtggccaatg cgtccatgac gaaatccatc gaccaggtca gattgggcgc 58620cgccggacgg agcagcggca gacgttctgt tgccagccct ttccgacgcc ttctgcgttt 58680tacgcccagg ccactgaggt gataaagccg gtacacgcgc ttatgattaa catgaagccc 58740ttcacggtgt tagcgccagt gatataagac ggtaattcac cattagtatt gtccgctcca 58800cccaacatgt tgtttccttg aggttctcac accagaaagg acatcaacat gctgagcaga 58860gaggactttt acatgataaa gcaaatgcgc cagcaggggg cgtacattat cgatattgcg 58920actcaggtgg gttgctctga acgaactgtc agacgctacc tcaaataccc tgaaccgcca 58980gccagaaaga ctcgccacaa aatggttaag ctgaaaccgt ttatggacta catcgacatg 59040cgcctggcag agaatgtctg gaatagcgag gtcatcctcg cggaaattaa ggcgatgggc 59100tataccggcg ggcgttccat gctgcgttac tacatccagc ccaaacgtaa aatgcggcca 59160tcaaaaagaa cggttcgcgt cgaaacccag ccgggatatc agctccagca cgactggggc 59220gaagtcgagc tagaggttgc tgggcaacgg tgtaaagtta acttcgcggt taatacgctg 59280gggttctccc gacgcttcca tgtcttcgct gcgccaaagc aggatgctga acacacctat 59340gagtcgctgg tccgtgcctt ccgttacttc ggcggcagtg tgaaaaccgt gctggttgat 59400aaccagaaag ccgcggtgct gaaaaataac aacggaaaag tggtgttcaa ctccgggttc 59460ctcttgttgg ccgaacatta tgacttcctg ccacgggcct gccgcccgcg cagggccaga 59520accaaaggta aggtggagcg gatggtgaaa tatcttaagg ataacgtctt cgtccggtac 59580cgcaggttcg acagcttcac ccatgttaac caacagctgg agcagtggat ggcggatgtt 59640gctgataaac gcgagcttcg ccagttcaga caaacaccgg aacagcgctt cgcgctggaa 59700caggagcatc tgcagccgtt gccggatacg gacttcgata ccagctactt cgacatccgc 59760catgtgtcct gggacagcta tatcgaggtt ggcggcaatc gttacagtgt tcccgaggct 59820ctgtgcgggc aaccagtctc gatacgtata tcgctggatg acgagctgcg gagctacagt 59880aatgagcagc aggtggcctc acatcgactc tgttcagcat cgtctggctg gcagactgtg 59940ccggagcacc acgccccgct ctggcaacag gtcagcatgg tagaacatcg cccactgagt 60000gcttatgagg agttgctgtg atgcatgaac ttgaagcgct gctgagtcgc ctgaaaatgg 60060agcacctgag ctatcacgtt gaaagtctgc tggagcaggc ggctaaaaaa gagctgaact 60120accgggagtt cctgtgcatg gcgctgcaac aggagtggaa cggcagacat cagcgcggca 60180tggagtcccg actgaagcag gcgcgtctgc cgtgggtcaa aacgctggag cagttcgact 60240tcaccttcca gccgggcatc gatcgtaagg ttgtccggga gctggccggt ctggcgtttg 60300tggagcgctg cgagaatgtg atcctgctgg gtcctccagg tgtcgggaaa acccatctgg 60360ccgttgctct cggcgtgaaa gcagcggatg cagggcatcg ggtactgttc atgccacttt 60420atgaagacat tactaacatc ggggtgtact aatcaacgag gagcaggtca ggaaatagcg 60480ataacctata aatttgtggt gccagtaata tcagatttcg ctcagtaaaa gttaacacca 60540agttggagtg ttgactttta tctcctgttt ttttttaatt tttaatgtaa aagacttgta 60600ttaagtccat tcacttggtg gcgtcgccgc ctcctcattg gtaatgagga gtaccggaag 60660agttctcaat gtgaatggtt gagctgtata agtttgttat actttatatc cacggtgata 60720caacgttggg gtagcatctg ggaaggagag aaataaaaaa attctatatt tttctaattt 60780taaagggtca gttatatgcg cacttacagt tcattacttg aagaatttgc tacagagcta 60840ggtcttgaag aaatagaaac aaatgagtta gggcatgggg ctgttaccat tgataaaata 60900tgggtagtac atctggcacc tatcaatgag aaggagttag tggcttttat gcgggcaggg 60960atcttgactg gtcagtctca actttatgat attttgcgga aaaacctctt tagcccacta 61020tctggtgtaa ttcgttgtgc gcttgataaa gatgatcact ggctattgtg gagtcagtta 61080aacattaatg acaccagtgg gacgcaactg gcgagcgtat tgacgtcgct ggttgataaa 61140gctgttactc tttcttgtga acccacaatg aagaaagagg aggatgatca tcgtccttca 61200tcatctcatt tactggttta attctataaa agaaaaacgt acgatatcca ttaatgggtt 61260tggttgagac tgtaaacaag attgtataat tgcctgtttt tgatatcttt actccaacaa 61320cggagacagg caaatttgat ccctccccaa tatccgtacc aggctaaatc agagatccgg 61380acctttttga tgacttcggg caaattctgc cggagtcagg ttatttaacg aagaatgcgg 61440atgaaaatga ttatattctt gccgccattg ttcaattttc tcctgagcat cttccagaga 61500aaggaacccg tgcacgttca gacattcatc cctcagactg ccattaaatg actcgataaa 61560ggcattatct gtaggctttc cggggcgtga acagtccatc gtgaccctgt tttcatacgc 61620ccatcggtcc atcgacttcg agatgaattc gctgccgtta tctgtctgca gcctttgtgg 61680aatacgcccc agcgaatgtt ttaatctgtc catgacagcc acaacatcat ctccacgtaa 61740cccctgaccg acctcgatcg ccagacattc acgactaaaa ttatccacta tagttagcgc 61800cctgacccga cgcccgttga acagattatc agcaacgaaa tccatgctcc agcactgctc 61860taacgcggtc acttctggac gtgcgtgcct gtgcttcgct gtcacatgcc gccgtggacg 61920tttcgaacgt agattgagac cctcaagaca gtaaatacgg tgcgttttct tatggttaac 61980aagccagcct tctctccgca gcaggatatg aatgcgcgga cagccataac ggatgcgcgt 62040ctccgcaatt tcccgtattc gctgagtaat agcccgatcg tcacgatggc tacgatagtt 62100gtatacagtg cggctctgca tcatcagcct gcaccctctg cgtactccga tacggtacgc 62160cgccagcaga tattccacgg catcacgctt ctgcagcggc ctcagaactt ttttcggatg 62220acatcctgca gcatttcctt atccagactc agatcggcca ccagacgttt gaggcgctga 62280ttctcatcct caagctgccg aagacggcgc aattccgtga cacccattcc cccaaacttc 62340ttcttccagt tgtaaaatgt cgcttccgaa atgcccatct tcctacagac ttcttccact 62400cgagtacccg tttcagcctg tttgagtgca aaagcgattt gttcttcggt ataacgcgtc 62460tttttcataa cgaatgaccc ctttttggac ggaaaagaag ggccgaaaac tctactttac 62520agcggtactg aactaagggg gaagatcaag caatccttgt ttgtcgatta ttttaaattt 62580gttttgactc ctccaatctt tgattcaata gacacgcatt caatggtttt tattatatca 62640tcaatgccgg cccagagatt ttcattatta ttgttcttgt aatttgtttt taattgagta 62700ttcaaactcc tctcaattcg catcacttca tcaatatgtt caagcttaca ctttattaag 62760tcatccctaa gagaaatatt ttccttctca agtttagata ttcttttctc tagccgagtt 62820gttatttctt tttcctttct atttttctct ttgttgaaaa ataaatcaaa gtagtatttt 62880gcaaaaaatg ttaacattac actgagtgtg taaaccagaa attgaatatt tgatggtcat 62940gtggatttag gaatataaaa cccaaccgaa gccataaaaa tagcgggaat tgcatatgat 63000aaaaatgtat aaatagtcat ttttatttat ctgctttagt ttacatagtt gtgttctatc 63060cctttgtctt taaagctatt acttagcttt ttaggggcaa ttctaagaag tcccatagat 63120gtttctagtt cattggaaaa atttggaata ccctttgagg catcaaacac catgctatat 63180gtaaactgaa ccgtaagttc ttttctttca acgtctagcg aatttaaact tgccttaatt 63240ccgagacata ctgagttaaa ggaatttgtt gctttatata ctgaaacttc atttggcttt 63300ccattgctga aaaagtcaaa gttctcgaaa gtaaaaagat atttaatttc ataaatgttt 63360aaactgtcag ttgtgaggtc tgaaaaataa taatcacctt caatataggt aaactcacca 63420ttatttttat caagcatttt tatattctta agagattcca ctttttctaa aatagagtat 63480aaattattct gcaaattcat tttacgcccc ttagctgttt attgtgattc attgatgact 63540ttataaatag ttgaacgagc tatattcatt tttttggcta tttctgtcgc gcccatcccc 63600tgttcatgca attcgagtag ttcctttctg ttgattctgc gctttcgtcc gaatttaacc 63660ccctttagct tagcctcttc tctcccttca tttgtacgct ccagtattct ctgtcgttcg 63720gcttgagcca ctgctgatag aatagtgaca accattttac ccatttcccc atcggtactg 63780attccgtcat caataaactg gatggacaca ccctgggcgt caaactcttt tatcaactgg 63840atcatgtcga cggtgtcgcg gccaagacgg tcgagcttct tcactagaat gacgtcacct 63900tcttccacct tcatacgcag cagatccagc ccctccctgt cggcagagct gccggatgcc 63960ttatcagtaa atatacgatt tgctttcacg cccgcgtctt tgagtgtttt gatctgaata 64020tcgagggatt gctgactggt tgatacccgc gcgtaaccaa aaagtcgcat aaaaatgtat 64080cctaaatcaa atatcggaca actggtgtct attataacaa aaaatcgatt aaatagacac 64140acaaaccgca ccatttcagt gtgtccgaca acttataata tttcggacgg ttaaaaagtt 64200gttaacaaat aaccgtcagg cagggaggcc tgtatgccag tcgattttct gaccactgag 64260caagaacaga attacggttg ttacgttgca gaacccaatg acgtgcaact ggtgcgctat 64320tttcatcttg acgagcggga tcttgctttc atttaccagc ggcggggaaa gcataaccgc 64380ctgggaatag cacttcagct cacaacggcc cgttttctgg gaacctttat tacggattta 64440acccaagttc tgccaggtgt tcagcatttt gtcgccgtac agcttaatat acgccgtccg 64500gaagtcctct cccgctatgc cgagcgtgat accactcgca gggaacatac cgcgcagata 64560aaggaatatt acggctatca tgaatttggt gattttccgt ggtctttccg cctgaaacgt 64620ctgctgtaca cccgtgcatg gctcagcaat gaacgccccg gtctgatgtt tgattttgcc 64680actgcgtggc tgcttcagaa taagattctg ttgcccggag caaccacact tgtacgtctc 64740gtcagtgaaa tacgcgaaag ggcaaatcag cggctctgga aaaagctggc cgccattcat 64800tatctgaccg aactaaacgg cacgaaaaaa cgcctcctgg atgatgctcc tgaacatatt 64860attaccggcc cctggaaacg cctggtgtac gatgcggagg gccggataca acgtgccggt 64920tactcgcttt gtctgctgga gcgccttcag gatgcattga gacgccggga catctggctg 64980aaaaatagcg atcgctgggg agatctccgc gagaagttgt tgcaggggga agagtggcag 65040gctcagcggg tcctcgtctg ccgggcgttg ggacatccca ccgatggaca taaaggcgta 65100caacagttgg cggtccaact ggatgaaacc tggagagcag ttgcatcccg ctttgaagga 65160aatacggaag tccatatctg caatgacggt aaatatcctt ccctgactat cagcagtctg 65220gagaaattgg aggagccact gtcgttgctt cgtctaaaca atcgggtcag gcaactgcta 65280ccgccggtag atttgacgga actgttgctt gaaatagatg ccagaacggg atttacacgt 65340gagtttacac atgtcagtga atccggggct cgagcgcaag atctgcacat cagcctgtgc 65400gcggtactga tggctgaagc ctgcaatatc gggctggaac cgctgataaa gcacaatata 65460ccggcactga cgcgccaccg gctcagttgg atgaaacaga attaccttca ggcagaaacg 65520ctggtcagcg ccaatgcccg gttagttgat tttcagtcca cgctggagct tgctcgccgc 65580tggggtggcg gcgaagtggc ttcagttgac ggtatgcgct ttgtcacgcc agtgaaaacg 65640gtcaattccg ggccgaacag aaaatatttt ggctccgggc gtggcatcac ctggtacaac 65700ttcgtctctg atcagtactc tggattccac ggcatcgttg tccccggcac attacgggat 65760tccatttttg tgctggaagg ccttctggaa cagcagacag ggctgaatcc ggttgagatc 65820atgacagaca cagccggtac cagcgacatt atatttggcc tgttctggct acttgggtat 65880cagttttccc cccgtctggc tgatgccggt gaagctgtat tctggcgagt ggataaatcg 65940gcaaattacg gagcacttga tgagcttgct cgtgggtgtg cagatctgtc gaaggcagaa 66000aatcagtggg atgagatgat gcgaactgcg ggttcgctca agctgggcac cattcatgct 66060tcagaactca ttcgctcact actgaaaagc tcacggccgt cagggctggc tcaggccatc 66120atggcggtgg ggcgtgtaaa caagacgctg tatcttctta attatattga tgatgaagat 66180tatcgtcgcc ggatcctgac gcaactcaat cggggagaga gccgccatgc cgtggcacgt 66240gcaatttgtt acgggcagcg cggtgagatc agaaaacgct accgtgaggg gcaggaagat 66300caactgggcg cattggggct ggtcactaac gcagtggtac tgtggaacac gctttatatg 66360caggaagcac tgagctggat gcgcagtaat ggagaagaaa ccagggatga ggatatcgcc 66420cggttatctc cactgatgca cgggcatatc aatatgctgg ggcattatac gttcacgcta 66480tcggatgata ttttaaacgg agaactgaga gcattaaatt tcaatttaaa caatgaatta 66540tctccttaac gtacgttttc gtcccattgg acctcaaaac ccatcaccgg gtagccacgg 66600ttgaccacaa tgcgatccag tactctgatg acgtgctgtg ttggcaggtt cagatcgatt 66660tcaatcgcca gcttttcacg attgtaatca tccacacaga tcagtgcatc gtgcataaaa 66720tcgaccgccc cgctcaggtt aagtcgttcc ggcaccgcca gcggtgatgg ggtacgagct 66780ggcaggcgtt gtttcccttt gcggcgaata ttgagtttca ggagacaata gatacggtgt 66840acccgtttat ggttccagac atatccccat cgcctcagga tttgaaaaac cttaaagaaa 66900ccgtcgcgtg gataacgctc gaccactgat ctggtgttca gtgaatcagt ttttttcgca 66960tggtgaactc ctcaaaatac atattcagta tgtcggaaat tctctaaaag agaatggtta 67020tttttgatgg tcattacagt acagattatt ttatataatt tattacccac tttttttata 67080ttttttgata gagggaggat aataaataaa aaaattataa agtaatggtt tcttgctgtt 67140ttctggtgtt tttctctata tttatgtttt tttggtgaaa ataagtgtgt tgtcaggtaa 67200tttcaaagga ttatacaaaa tatccggttt gaggtgaggg attttttttt atattatctg 67260cataacactt ttcgtgttat ctgaaagtat tttgtagtgg gctgactccg acgattcgat 67320tagagattac aaacgatgca tatattcagt agttaatcga tatcttttta agatcgatta 67380gtgctgtttt ttgcatgatt atcagaaaat aagtcataga taatcctatc cctcttctat 67440gggaggcgtt cgctttaatt aatatatttc tcagatgtta taactgagct tttattcacg 67500ggaaattaaa gaaatataaa aggtgcttac aatgactaaa gattttaaga tcagtgtctc 67560tgcggcatta atatctgcgt tgttctcatc tccatatgca tttgccgagg agcccgagga 67620tggcagcgat ggtattcctc gtttgtcagc agttcaaata agcccaaatg ttgatcctaa 67680attgggtgtg ggattatatc cagcaaaacc aatattacgt caagaaaacc caaaattacc 67740tccacgaggt ccacaaggtc cagaaaaaaa agagctagat tagcagaagc aatacaacca 67800caagtactag gcgcaggcgg gctcaatgct cgcgctaagg atccctatag cattgcgatt 67860ggtgctactg ctgaagcagc aaaaccagca gcaattgctg tgggctctgg ttcaatggca 67920acaggcgttg attctgttgc aattggtcct ttaagtaagg cattgggaga ttcggcagtt 67980acttatgggg taagtagtac cgcccagaaa gatggagtag ctatcggtgc gaaagcatca 68040gcttcggata ctggtgtcgc tgtcggtttt aactcgaaag ttgatgcaca aaactctgtt 68100gccattggac actctagtca cgttgcggca gatcatggtt attcaattgc aattggggat 68160cattctaaaa ctgaccgaga gaatagtgta tccattggtc atgaaagcct taatcgccaa 68220ttaacacatc ttgcggctgg cactgaagac actgatgcag tgaatgtcgc gcaattaaag 68280aaagaaatgg ctgaaacatt ggaaaatgca cgtaaagaga ctttggctca gtctaacgat 68340gttttggatg cggccaaaaa acactcaaat agtgttgcca gaacaacttt agaaactgct 68400gaagaacatg caaataaaaa atcagctgaa acgttagtaa gcgctaaagt gtatgcagac 68460agcaattctt ctcaaacact aaaaactgca aatagctata ccgatgtgac tgtaagtaat 68520tcgactaaga aagcaacccg tgaatctaat caatacacag atcataaatt cagtcaactt 68580gacaaccgtt tagataaact tgacaaacga gttgacaaag gtttagccag ttcagccgct 68640ttaaacagct tgttccagcc atatggtgta gggaaagtaa actttactgc aggtgtcggg 68700ggatatcgtt ctagtcaggc attagcaatt ggttctggct atcgtgtaaa tgagagtgtc 68760gcatttaaag ccggtgtggc ttatgccggt tcctcgaatg tcatgtacaa cgcatcattt 68820aatatcgagt ggtaatatca tttagaaatt aacaagtcta taggaaaaca ccgattacat 68880aatcgtaatt ggtgttttat taatatgcta atgaaaaatt ttttagtaat tctgcttttt 68940atcatggttt cagttacatg gggaactaca tggttagcga tgaaattaac cgtcgaaaca 69000atctctccga tatttgctac gggcatccga tttatgttgg ctgcgcctgt attaatccta 69060attacatcaa cccgcaaatt cagcgaagca gagtaaacac tgcgcgttat tttgagcagg 69120cggcacaatt cgaccactgg ccattttgtt ttcagccgtg ttaacgcaaa gatttgatgg 69180ggaactcgct catcaacacg gctgcctgct ttagtatttc tttttccatt tccagccgtt 69240taatctgcgc cctgagcgac tggatttcac gttgctctgg tgtcagtgca ggattgtccg 69300gcgtcacccc gctgacttct tctttgtact ggcgtatcca tttacgcaat tggctgggat 69360ccagctccag agcgcgtgca acctcgatgg ttgaccgctg atacttaacg acctgctcaa 69420tagcttccag tttgaattca ggagaaaaac ggcgttatcc agaacgtgaa gcgatttacg 69480cggcgctgga gtaaaccaat gggcggcatg gccgcccatt ggtttactta tcagtacgca 69540gtgccatttt gcggccgcgc gaaaaccctt gtcactctaa gtaacgcgga gggtgagtcg 69600gtactgattt ctccgcagca gaataccgcg caggatattt ccctgttcat gccccgggaa 69660ctgacggtca gtcaaggtga tcgggtgcga tttacccgct cagacacaga ccggggttat 69720gtggccaata gtctgtggga agtggcgggt tttactgaag acggtgctgt gcgttttcgt 69780cagggcgacc aggaaaagat tgtcgatcca caaaaggcca ccgaagaccg ccatattgac 69840ctggcctaca cgctgacagc ctatggtgtg caaggggcca gtgagcggtt tgtcatcgcc 69900ctgtttgggg ctgaaggtgg cagaaagagg atggccactc tgcatctctt tacgtgacat 69960tgtcacgcgc caaagagcat gtctaggtct atacggacaa cgttgttaaa tggttgggtc 70020ttgccgggca gtcaaatgcc ggaaaaaccg cgcattgtag tggatcagta ataccggaca 70080ccctcaatag cctgttaatt taatgacagc caattgaggt aattgataat gactcaacct 70140aaacagacca aacgccgttt ttctcctgaa ttcaaactgg aagctattga gcaggtcgtt 70200aagtatcagc ggtcaaccat cgaggttgca cgcgctctgg agctggatcc cagccaattg 70260cgtaaatgga tacgccagta caaagaagaa gtcagcgggg tgacgccgga caatcctgca 70320ctgacaccag agcaacgtga aatccagtcg ctcagggcgc agattaaacg gctggaaatg 70380gaaaaagaaa tactaaagca ggcagccgtg ttgatgagcg agttccccat caaatctttg 70440cgttaacacg gctgaaaaca aaatggccag tggtcgaatt gtgccgcctg ctcaaaataa 70500cgcgcagtgt ttactctgct tcgctgaatt ttcgggttga tgtaaaacgt ctgcaactg 70559 2 100990 DNA Yersinia pestis 2aaacagcccg gcgtgctgga gcgactggaa cgtgaggacg gtgtcattat ccaccagcgt 60cgcgagtggc gcatgtacga tccggaaaca ggtaagctca cgacgaaggc cggaacgctc 120tggggtctgc tgaagaaaat ccactgataa caccaaccac tgcggtgagt agccagctca 180ccgcgcgcgt atctgggtca taaccactgt agtgagtaaa acggctgccg tggcatccgg 240tatccactgt agtgagtaaa gtggtgatta tcgacttcac tatccactgt agagagtaaa 300caggcgttca ttcacagcaa acaaccacta tggagagtga tggaatgcga cctccagcgg 360gtatccacta tggagagtaa accttcactg ttttcagcgg atgtctactc tccacagtgg 420atagtaaatc cagccaaccg attctgctct ccatagtgga tagccaatag cgaagggagc 480aacgataacc actatagaga gtggatttaa caagtcaccc agtgaccact aacctcgcag 540cccttgtttc atctaggttt gtaaccacta acattcattt cgttatttga gcgctactgc 600ctacagtggt tactattcgg ttgttgttac tcactacagt ggatagcgga cttcagataa 660acaaaaggcc cactacagcg gaatagtgag cctttctact ctctacagtg attgggctat 720ttgcgagcct ttgccttgcg cagctcttcg agaatcgcca gttcttcctt gctcaacatg 780accatctcac cgtctttttc ttcaggaaca acatcgatga tgtcctcctg accatcgcca 840ggcaagttat cttcctgctc ttctggttct tccggcgcag ctttagttgg tggcaatgcc 900gggcgtaact tcggccgcct atagtggatg atgaagtaga ccgagctgcc gcgcttcact 960tcggtgtaat cgagatagcc gatctcccgc agctgctcca tcgccttcct gactgtcgcg 1020ttctgggtaa tggtgcggct ggttaagtta agtctggcgc gtaagcgagc caacgagatt 1080ggtgccgggt caggtggcaa actttcgatg aatgtgtaga gtgcctgggc ggattctttt 1140ctggagagtt cgttgattgc ccggagttgc agaagaacct ttttgtcgaa ctggtagagt 1200tcgaaaatct taggatcagc ctgcagcgag accgtgtcgt tcttagtgct gtactttgct 1260gtctgcacaa ggtgagttac gtaatactca tcagagcctt tactgcggaa tgagatagtg 1320tttgtggcga tacgagttag agaactgtcc aggcgcttac gtaacttcgc ggacgatctg 1380gccgttggta tgccacagag cctgacgaac tcgacgaacg gtaacgtgac agtgtcgcca 1440acaaccttgt gcttggcgaa cgcgtggatg atgcctaccc acgttttaaa gtcgttatcc 1500atatccagac gaacgccgga gatccttatg tcctcatacc cttcggcttt ggccagagac 1560agctgtttga gttcagcaga ggcgtccata gagaccattt gccccttcct gcccctggac 1620gtcgatttca gcgtcggaac gaagagacca agacgcatca gagcaacggg ctgaacagtg 1680ttgttggtgt taggaactaa cgtaacaact tcgcctgtct ttttgtctgt ttctgagaat 1740gcttcaacga tcgctatgtt tttattgccg ttttcgctca ttccaagtgt ctctttttat 1800tcgacggctt tggtggcctt tgctgattac agtggatatt agcactcatc acagcggtta 1860tcctaccgcc tatagtggtt tttctactct ctacagtggt tggtttactc ttcatagtgg 1920ctgatcttct ctctatagtg gatatcgatc acctctgagg ccagatggca caacggtttg 1980cgggatgcgg ggatcttttt gggtctttgt gggtctcttt ggttctattt gggatctgaa 2040ttactggatc gggcctgtgt ataaaaatca ggtaattgca aaaccagcca tcactccctt 2100tctggggata atgtgtggtg gaaacaaaca cgcctgaacg caagattaac gatatatcca 2160tgaatatcag ctatctaaca cagaaaaaca ggcaaagagt tatccagtca agacaatgcc 2220tcctactcac tacagtggat accgaccact ctccagagag gttgttttgc tcttcatagc 2280ggttattatg ctctctacag aggttacttt gctccccata gtggatagta accccctctc 2340aggccagtaa ccgcaacggc tggagacgat cggggatctc ttttgatctt ctgtaggatc 2400tctctgggga tctaattatt ggatcggtcc tgtgaataat gggtataagt aaaacaggca 2460tttgcaaaca tcggtgcgcc ttgtcagtta tcgttgcccc ggacaaaact atttgaataa 2520gaattatgga tctcaaacgc acgcgctggg ttcgccgtct tgaagatggc tcctacacca 2580ttgaatcgaa cactatcctg aacaaacaga aattgctctg cgacctatgc ggcatagcgt 2640cgaagtgccc gattaacgaa actcggctca aactctacga cgccggtgcg cacttccact 2700tgaacagctg catacgttac gtgccactgc tggcatttcg taaaccgatc atcggattgg 2760acgcacccta cttcaacacg ctccgctctg gtgtaacatg gcgagaccgg gtcgaacctg 2820gcaagctcgt ttgtctggtt gaggctgata ctggaaatat catccggttc gggagagtcg 2880ataaagtcta ctccggcccc gtggatgaga tgctgcggaa acacagccgg tttaaccatc 2940tctgcatggg cggagagaaa attgataagg ttggcgaagt gatccgcaaa tcctacggac 3000acttcctgaa cgacgacagc cttctcacgg ccatctacat tcgccatgtc gatcgggagt 3060tcgatacgga gtatcacagt gctgaagagt tgaatcttgt cgaccctcgc ccaaaagctg 3120gggtaatcga catcagcgta gcgcgtcaga agccctctga gacgttttaa acgtaaactc 3180atggttttac cggggcataa aatatggtgt cttagaaaga cactgagagc tttagggatt 3240aatacatgag tgaattttat tcaagggcgg cggctgtggc agaccaagct ggcaatgaag 3300acgaacgagc agggccggtt tttaatttgc agcccccgtt aatcatagtg acagtaaaat 3360atctgtcaca aaaacagtac gagagagatt aggatctcag tagtggtcag aagaaaaata 3420tgtactaggt taaaacaggt acatgtttac tggtacgcat cacataaata atagtaatgc 3480actccaggta aataggcgat tgtccagtag acaaccgcct gtaatgagac tatttgcctg 3540tttcttgctt gtttaagccc actgctttct gataatagct ggtatcataa tatttatgcg 3600tatctgtcag atttttctct ggcttagcaa gcctgcttcg caacagtaga acatttttta 3660ccccttctgc attaatagca gcatcccgtt gcagcccatt gttaagtaat tcctgtaatg 3720caggtcctgc cagctccgcc gtcattcctg gtgccttttt gaccagaatt tcttctgctt 3780ctttttggtt acgggggtca taaatccagt tcagtccgtc gatatagcta cggatgtaac 3840ttaccagaat atcaccattc tgagccgccc aggagcgggt tgtaatgcca gttgtgccct 3900gatagtcgcc aagttcactt ccggaggcaa ggatcttaaa cccattttct ttcgcctgca 3960gatttaatgg agtacggaga agtgttgcat ctgtcttacc ggcgagaagt gcattaaagc 4020gatcattcgt actgccaaca cttgtatagt ggacatcatt ttgtgtcagc ccgtttttct 4080caagataatt ccggataaca aaggcatagc cagtggtcaa agcgtcgact gaaacttgtt 4140ttcctttaag atcactgatg ttcttaattt gagggttagc aactaatgaa agaagcccgt 4200tatccactcc ataaaacgcg aacatatccg gattcactac cggctctttc acctggccct 4260cttgatacgc gatgacgtta tcaatacctg ctactgcaat attatacttg ccattcagca 4320ggtttctgac cagttgaccg gaattgggtg tgtaatccat tttcacatta agaccatttt 4380ttctgaaaaa gcctttttcc tgggcaaccc aaacaggcag gttccacccc ccctgaaaag 4440taatgacgtt aatatccctg atagatgagc ttgattcccg ggtatccgcc gcgtgtaggg 4500cgccgggcag gataaatgca agtgaaacgg ttaaagccaa gcatctgaac ataatattta 4560cctcttactg tgaacagttc gtaagagttt aaaaaaatgg gtaatgataa taaagtagaa 4620agcattctgg ctatctggcg aataactggc tcatttttac cagcaaatgg ataagcataa 4680gcgccggtaa ggcagcctcg aatgcgcaga tctcggtcca ttgccagtga cgaaagccca 4740gactgctgag agcggattct agagacatgc ctataaattc tgacgtgacg aagagttgct 4800cgttacattt agaaatcatt accgccatga aaataggtat tcacttactt atgtattttg 4860tcataatcat gtgcctgtag attttcttct gtgccgtgtt gtctgggttg ttcgcctctc 4920aagcacgctt aatatctgta tcaaaataac cacaaaggaa aagacacatg acattgccat 4980acggggtgat atcagatccc cattatcatc gttgggatgc ttttgcgaca acaaacgctg 5040acgggctgaa ctctcgactg gagatccaac tggatgccac gaaagaagct gccaaagcca 5100tgaaagctgc gggctgcaag cacatgctgg tggctggtga tactttccat gttcgtggtg 5160ctatatcgcc ttccgtcctg catttcgtga ccgaaactta cgagtggatc atcaaagagt 5220tgggcctcga agtggttatg ctggccggca accacgacct cgaaaccaac gattccgtat 5280acagcgccaa tgcagcggcc tctctgcgct caatcggtgt ggaaatcgtc tgcggcaaac 5340gtcctcactc catcaaaatt ggcgacgtta ccgtccatct gattagctgg cgcaataacc 5400acgcagagct tatcagcgac ctcaaaacac tgcgttccgg gctggatggc gacaatcacg 5460atgtcgttgt gcatacctcg atcaacaaag cgatccctac catgcctgat gtcggcatcg 5520acgcacagga actgaaagat atcggcttcc gtttgttgtt gtccggacac taccacaacc 5580acaaagaagt gctgcctggg gtggttagca tcggggcgct gacgcaccag aattggggtg 5640atgttggctc gctggctggc ttcatgatcg tcaaccctga cggcacattc acccaccacg 5700aaacctctgc acccaagttc gtgaaccttg aggacgatgt ggaagacgat caaattcgcg 5760gtaactacgt gcgctttcgt gccgttgttg agaacgatga agaaggcatc aaactccaga 5820acgtcctgaa aacaatgggc gcgaagggtg tcgtctgcaa cttcatccgc aaggcatcga 5880tgatggaagg ctctgccagt actgcggaga ccagcaaaat agacagcctg ggcgagtccg 5940tcgcggcgta ctgcaagatc gttcacgaca ctgatggcgg cttcgacctg agcaagctgg 6000acatgctgtg tcaggaaatc ctgaccgaag cggagagtgc ggaggcagtg tgagtacgaa 6060ccattctggg agctttcggg acttcgtctc cacaatgaga agacttgaac gaggccagac 6120ggtgatgttc cacaagccct acccacctaa tggaaaccct gtggcgtttt acctgggaag 6180attgagcaaa aagggcgtac taaaacgcaa atccttcccg gctcacacgg agtttcaact 6240acgaaaaggc cagcatttga atcaaaaagt ttgaggcatt gtatgaaatt tctaaagctc 6300caggttgaga acttcatggc gttagccagc gccgaagttg agttagacca acgcggtctg 6360gtgctcattc agggtgttaa cagtggcgac tcttccgctg ccagcaatgg cgcgggcaaa 6420tcgactttga tgaacagcct gatgtggtgt ctgtatggcg aaactgcgca tggcgtcaaa 6480ggtgacgacg tgctgtctac aggtcacgaa aaaaactgtc gtgtgatggt aactgttgag 6540gatgaaggaa agcgttacgc catcattcgc caccgcaaac acaaagagtt caagaaccgg 6600ctgatcgtcc gtggcgaaga cggtgacatg accaaaggca aagacacact gacgcaggag 6660ttcgttgaac gcctgattgg tgcatcgaaa gaggtgttca tggcgtccat ctacgccagt 6720caggaagcaa tgccagatct gccgggtatg tccgacaaga acctcaaaac catcgttgaa 6780gaagccgctg gcgtcgaccg gttaacgcga gcctatgcca ttgctcgcga gcgtgctaat 6840gcagctgccg cacgcatgga tgttaccaaa tccaaaatgg acgcctgtct cacgcttatc 6900gagaccgcgc agtcagagat tgaggcggcc aaagcgtcct ctgatagttg ggaacgcgat 6960cgcggcgaac gtctggacaa ggcccgcgta gatttggctg gcgcggaggt aacgctgtct 7020gaagtcgtga tggaaattcg ctcgctgccg gaacagatcc gggatacgga aaacgcgatt 7080gctggcgaac gctgcaagct ggcctccaaa gaagagcatg acgccaaact gctgaaggtg 7140cgcggtgcga ttacggagat ccgctcaagc atccgcactt cagaagcggc acagaacgag 7200tcgatgaacc gtgctcgctc gtttaaaacc aaagcagaag aggtcagcac aaaggtcgga 7260gcaccttgtg ttacttgcgg aaagccctac tgcgaagaag atttgtccac cgtgaaggag 7320agtttcattg aacaagcgcg taatgagatc ggccaggcgc aagcatcagc ttcggcagtg 7380gctcaacaca aagctcgtct tgagaaagcg ctcggcatcg aatctgcact ggtcgcagcc 7440acacccgacg tttcagaaat catcgccaaa atcgaacgcc tgaccaatga gctaagtgcg 7500ctgcgtcatc gcgaacgtga agttgtggcc gtcgaagcga tggtggcgcg ggcgcgtacc 7560gatgtgaatc gcattatggc agaggtaaac ccatttctgg ccgttattaa gcgtcatgag 7620gacaacctgg ctgccaataa atctaatcat gcagtactta aaaatgagtt aaagagtatt 7680caagaacagg ctctgttgct ggagaaggct cgccaggttt actcccctgc aggtgttcgt 7740tcacacatcc tgacctccgt tacgcctttc ctgaacatca ggactgcgga gtatctcaac 7800acgctatcgg acggcaatat cgttgccgaa tggtcgacaa tggagacaac gaagaaaggc 7860gagtatcgcg acaaattcaa tataagcgtg accaaaacag gttccagcaa atccttccag 7920acgttgtctg gtggtgagaa gcgtaaggta cgtattgcgt gctctctagc cttgcaggat 7980ctggttgcca gtcgcgccag taagaatatc gagctgttta tcggcgatga aattgacgac 8040gcgctcgaca ctgccggtct ggagcgtctc atggggattc tggaagccaa agcgcgtgaa 8100cgcggcacag tgatgatcat ctcccacaaa gagatgaaat cgtggttccg ggaaaccatc 8160actgtcgaag tcaaagaggg tcgcagctat gtcgtttaac ttgagccgca cgcagttttt 8220gcagatgttt gccgtgatgc aatctataaa gctgataaac caccatacgg caaaagcagc 8280tgcgcctgca cttttgtgga aaaacgaaaa catcaatgac gaccagttct cggtattaac 8340cagtctgttg tcatcgactc cgttgatgcc gagcttggct atgttgccgt caggaagcac 8400tgcgccgatc cttgttaacc catttacgga aggtggatat ctcccacatt ctgggccggg 8460gttcgttgcg atacctgaaa ccggaacgct gaatatccaa gaaaatgcgc tcttcaatgc 8520aatggagacg cacatcagca ccgcattcac caatctgatt cgacacgcta acgcacgcgc 8580tgatcacgtt gcaatgcctg gtgctgcttt cgccagcgtc tctgttgact atgatcggca 8640cgcgccaatc tcaaagcggg cgaaactctg cttttacgag gagggatgtg aagtagcggt 8700tattaaagtt cttctccccc atgtattcag cgcgaatgaa aaggttgcac accatctgat 8760cgacatcatg cgacatttca tcggccagag catgattgat gcagacattg ctgcaggtgt 8820tctaaccaac gatagcattc atgttgttag cgacattccg aagccgccaa ctcgcgagcc 8880ggagaagaca cttgaacaga aactaatgga atgcccaacc tgggctacgt ggtaaggaga 8940ccaaaaaatg agtaaaacca ttcgtgtggt tggcgtcgac ccttcaatga gcaactttgg 9000cctggcgatt ggcacgctgg atctggaaac ggataagctg gacattcatg gcctgacatt 9060ggtggaaacc aaagctggtg gcaacaagaa gacggttcgc gtaaacagcg acgatctacg 9120ccgcgctaac gaaatctggc gcaccgccaa gcccataatc gagcaggctc atatggtgtt 9180ttgcgagttg ccggttggta gccagtccag tcgcgcacaa acctcatacg gcatctgcat 9240tggcgtactg gcgtgcgtgg ataagccact gatacaggtc acgccaaacg agattaagca 9300ctatgtcggg aataagctga ccacgtcgaa ggaagagatc attcagtggg ctacgcagaa 9360gcagccaaac gccccgtggt tgcgccgcaa gcaatctggt aaggaagtgc tggtgaataa 9420aaacgagcac cttgcggacg ctgtcgcgtc gatttacacc ggaatgcaaa ctgatcaatt 9480ccgtcaggtt cgcgatgtgc ttgcagggat tttataagtc gataattgat aggtaggtgc 9540ttatctatta acataaggcc actatattta gtggcctttt ttgtgcccag aaaaccccca 9600gctaggctgg gggttcagta aagctttcag ctttgggtca gttataaaaa ccccttttga 9660tttgttaaaa cagtttgcgg tctggcaact gcaaatgttc aacaagaaat caaaaggggg 9720tcccaatgag ggatgaaaag agcttagcgc acacccgatg gaactgtaaa tatcatatag 9780tttttgcgcc gaagtaccga aggcaggtgt tctacaggga aaaacgcaga gcgattggca 9840gtattttaag aaaactgtgc gaatggaaaa acgtgaatat cctggaagca gaatactgtg 9900tggatcacat ccatatgctt ctggagatcc cgcccaagat gagtgtctcg ggatttatgg 9960ggtacctgaa gggaaagagc agtctgatgc tttatgagca gtttggcgat ttgaagttca 10020aataccgtaa cagggagttt tggtgtcgag ggtattacgt tgatacggta gggaaaaaca 10080cggccaggat acaagaatac ataaagcacc aattggaaga ggataaaatg ggtgagcaac 10140tctcgatccc gtatcccggt agcccgttta cgggccgtaa gtaatccata gatgcaaatg 10200tcagatcgcg atgcgcctgt tagggcgcgg ctggtaacag agccttatag gcgcatatga 10260aaaacctccg gctatgccgg aggatattta ttatacccga taacaaaatg ttttttgcct 10320tatccacatt gcgataatta caccaacaag aaaacaagat gtttacgcat ggaggatatg 10380cacatgaccg atttcactat ctcccctaaa gctgaaaacg tatggctgga atcctggctc 10440gacctgtcat cggaagagaa gcgagaaatg gatcatattg aacaggacga acagtgtgat 10500gcccgcttct tccactttga gggcagcgtt tatgacattg ccgacttcat gcgcgatgac 10560cgattcccgg gctggcacgc aggctaccca ttaaatgcct tcgccatgct gatgatccgc 10620gtggatggct caggcgatac catcgacgtc ggtttgctcc actaagagaa cgaggccacc 10680catgctggtg gccttaaatg gccatcctgt ttcccgcagg ctaaaaacac ccacctctta 10740ccgccaggct accgaacaac cctccgactc cctgcaggcc accatctgcc ggaacagaac 10800gcttggacgc cttatgcgcg tagcgataat taaaccaaca agaaaacaaa ttgtttaaag 10860gattatcacc atgaatttta tcgctactgt taacacccct tcgcatggcc atatttctgt 10920gacgttctct gataacgata aaagcgtgct gggcgcctgg cgtgacaatg taaccatcga 10980gctgtccggt aaagagaaac agcagatcac caatgacatt atctgcaacc gtcgccataa 11040gcgcgtattt gaaaaagcgt atgtctccac ctcgggattt ggtgtattca tcttcccggt 11100acgcagcggt cgcttctgcc ggtcaaaact catcgagttc gccacgcaga tcgcgctatg 11160ggttaaaaca gaatccggat tcgactttac cgaacaggaa gcagtggggg aggggatgcg 11220catcgccaac aacgccatca agtgcaaaaa cgtcatctat gaagcaggaa tcgactcgtg 11280gagtatctcg tgcggggact acgtgaaaga ggtgtacgga aagaaccgca ttcacatcct 11340ggctggcaag taagagggga ggggctggaa acgccccttt cttttcgtcc accagttgcc 11400gcagggaaac ttcagaaacg gccagagagc tgtccgggga accgaaggga aacggccagg 11460gaattttcgg gaaacggcgg ggtttgcctt tatgtagaaa acagagcggg agaagccaaa 11520aatcgctcca gaaattgcgt agcggcgctg gggtagttgc cggtggagtt tcagctcctg 11580agccacccag atagctttcg ccatgtgatt atgtgaatcc gtgggaaagc cactgcaagc 11640gcgtacacgt cgcgtcaacg tgccaatgat acgcgagcgc ccacggatca tgccaatatt 11700gccgacacgt cccgaaggat agcgcggatc acgtcgccag atatcgccag acgatcacgc 11760ccacgacacg acaaaataag ccacgcgcta aaacgcgcta taacgtgttt tttattgtgg 11820gtaatgagta tgtaccacca cacataaaaa cgcgttaaat tggcgcgttt atggcgctta 11880tttttggtct gttttggctg acttcagaca ataaaaaacg cgccaacaat ggcgcgttat 11940ggtgtgcgga tcttgaaacg aaaaaagcgc ccatagtggg cgctattgtt tttatttttc 12000taagtgaatt ttaaagccag cgtttaaaaa ttcttgaatc attaaaagaa catcggattc 12060tttgattcct gcgcgcttcc tatgctccgc gctattcaga tctattttaa acgtggtttc 12120gtcgactact tcagagctga gagcgtatcc agctaaacca gcgatatcat aaacaagagt 12180atgattatgg atgttaacgc cagcgataaa aataaccata aaaacgctcc ttaaaaaata 12240aaattgaata cagacttaag atcttttgaa taagcgccca tagtgggcgc tatattcaat 12300taattacgct ttgaaagcgt cagccagata gttataaaaa tcatttttga taaagcgata 12360ttgctgcgat ccgtttttag cagcgcccat tcctttgatc ttctcgacca gtccgagacg 12420ttcacaaaga ttgattagtt ggttggcttg agtgtagcca gcgtccaatt taatttcgtt 12480ggcttttttc gcttcattca tcaaatcgaa aacagcgcca ttagtgaatg tttccaattc 12540atcattaatc atttcgatta atgcgaatac gcgagatccg gacatatcag cgacggaata 12600aacgcattta ccagacttga tagatttaac cagataaacc agtttttcga gtgaatagct 12660attggtcata gcttcacgga aaaacgcttc aggtgcttgt ttgcttgctt taatcgcgta 12720gtaaaagaca ccagctaatt tctcatcatt aacagcgttt aaaacgttgt tggtaaagta 12780tgcaagtttg gtagtcgctg caagcatgtt agctttatct gctttggtgt gcgtaccatt 12840ctgataatga ttgttataag tctgagtcgc attgttggct gcaacttgca attcattagc 12900gataactaca gcagcgtcga tgatagattt tttagagata gcaacgttag acatgatatt 12960aatccttatg taatattgat aacttaattt gttatttatt tatcgttagc gtgttcgctt 13020tcgatgtgac taattatcga tatacaaaaa ttaaaatcaa gagttttttg cgcgggaatg 13080aaaaaaaatt ttcttcaata aaaatcaaag tcttagaaat aaaacgcgtt ttctcgaagg 13140tgttgcctaa ataaattccc tattcggtca atcaccctta tatatttaaa acggaaccgg 13200gattagggga ggtaaatata acgggaagtg acacataaaa taataaccgg acttagccgg 13260ttattaccct tatagattta aaacggtaaa atccgttcga cccaatcgaa catgacgact 13320gtcttccgac cgtcccccat gttgagcgtg gcctggcaag cgtctacgcc tgaagacccc 13380ccctcaattt cacggccatc cgccatgtag acccttatag acttctgcat ctcatgagcc 13440tggcgacaaa ttttaaagaa atcacggcga gatggccgat tgtccacata gtctgggtgt 13500accgttgtcc gacccgtgaa atcgtgcgca atgccttctg tcacacctga ttcaatcgtg 13560ctgattcgct caagtgggag ccttatacga ttttctttgt cgaacggggc agggcaaagg 13620tcgactttgt tgcgcgacga catgagaccc tgaacgtaca tgcagaacac ctgaccatct 13680tccatcgtga cccttacagg aatgagagac ttacgccaga acatcagcgc tttctccacg 13740ttggagtaat cgcgcggcca gacttctgca ggaatcccgt aggtgatgtc agttttattc 13800gtcatatcgt cacagtgtcg ttggtaggat atcgatgtca cctgcgttgc tggtgaatac 13860ccggaagact ttcgtctcgc cagctttggt gatggtctcg cgttcctgac gagcagggtt 13920cagtgcgcac agcccggcgc cctcaagtga ggcgccaaca atccactgcc cggcatcaag 13980atggaacgtc gctttttcgc cggtctccag tttcgcaact gtctctccat taatgaagat 14040ggatgcgtcg cagcccgcac ctatcatacc tttgtccctc ataaccacca gcgtggttgg 14100ggcagacgtc tggtatttga aaactcttgc ctgcggagcc ggttttgcat tggctacaga 14160cactgggcgg gatgaacatg cactcaggag taaaacgggg atggtaatca gtggaagtag 14220aacgtgtttc atggcttgaa ctaaatcctt atcacttcaa cgaacaaggc gtcttgcgac 14280gcccttaatg cttaatcgag acgtttgaga atatcggcca gatcttcttt ggtcatgcca 14340gaggattcgt aaatcttcat gaccttttca cgagcctttg cagaagcctc taatgacgta 14400gccgccttat caaaatcggc catcgtcatg ttggacagta ccagattgat gacgtctgct 14460tttgacagct ttatgttgcg ctcacgcagt cgattctgaa aggtttccag cttgtcgtta 14520gctttttcgg ttaactgaac ctggcagtgt atagcgcgtt tctcgctcat gcttactctc 14580tattcaaaac agtaaaatcg aatgtgctac ccaccggcaa gactccttcg gcaaaccctg 14640gcgtcgtgtc gataatgtgc ttccgctcat atgaatgcga catgaggtat ttattgctca 14700cgtcgatgaa gtcggtaata aagcacacgt tagcctgatt ctttttggct cgaagaccgc 14760gaccaactcg ttggcgcatc tcaacttcgg ctttgccacc gccacccaga atcaccgcac 14820ctacgcttgg aacgtcgacg ccaacatcca gaatggttga accaatcaaa acatctatcc 14880tgcctgccgc cagactgctg agctttgctt gtcgggtagt ctggtttgat tctccgtaga 14940tgaaatcgac cttcaggccg ctttccttca tcatttccat cagaatctga ccatgacgct 15000tcaaacgaac cagcgtcata cagtttagac cgtgactttt gtacattaac gcttcgcgca 15060caatggcctc gttgcggccc agattgtaaa cgatgcctaa ctgataggct ttctggtaag 15120ccgtactcat cccaacccga aagttaaggt gtttcgaagc aagttcggcc ctgattcgca 15180cctcgtctgg agtgtacgcg attttatgat atagaaagta gggttttgct aaaatacctc 15240ggtcgatcaa atatttttcc gtcaccttta tctcaatgcg acctgcaacg gccatgagac 15300gcatatttgc ttcggttgag tccttcatga acggcgtagc agtcagcgcc agacggtagt 15360cggcattaat gcacaaccgg gcgatatcgt agaagtttga accagatgat tcgtgtgcct 15420cttccagaat cagcagagaa acgctggaca ggaagcgctt aaccagttcc cggcgcttca 15480ggtggtaccg ttttttctct ggtgaggcat cgcgcggcgg ctcttcgaga aaactggcca 15540gggtctgcac cgtggcaacg ttgatatggc gcgagacctg gaactcacca gatccaatca 15600ccccaacttt ctgacctttc agccacggct cgccattctc cgcgcggtag tcgattgatt 15660tctggaagtt ctctgccatc tggaacatca gaaccgagcg cgtggttaaa aacagcgtca 15720tacgaccaat gcgagcagct gccttacacg ctacgttcga tttaccgcca cccgtcgcaa 15780tctgggcaat catcatccct tcgcgcacca gtgtttccac agtctgatcc tgatacgcat 15840agtccgggtt atacgggaat gggttaacta ccgggttcgg cttgcccagc gcgggggctt 15900tttccttgcg cacatgcacg catttgatgc cagctttcag aaggttggcc gctactggct 15960tcgcaaaccc agccgggaac gcgtttttgc tccagttgaa catcgtgctg gtccctttcc 16020agtcaccagc ctccacttca tagctcaaca tctcctgaac gagccgcttc acgttgtcat 16080cagcgccaga aatcagcgca ttgactgcat tcgatacaat ccgaactgtc ataaacctct 16140ttccttcgtg ccttttgtat ggtaattggc tattatagta agtaagtact tatataatgg 16200attgtatcag aattatggat gtgaaaatta cgattctgca ggtggaagtc gcgaacctgc 16260gtccgaatcc ctggaatacc aactccgttg gggcgcaaaa cttcgaaaaa ctgaaaggct 16320ctatcgaaaa attgggcttt tttaagccaa ttctggcgcg ggagctggac gggggcattt 16380ttgagatcct cggtggcgaa caccgctggc gtgccgcgat ggagcagggc atttcaacgg 16440ttcccgtcat ctccgtgggc aaaattaacg acctggtggc caaacagatg tccctcgtcg 16500ataacgagcg ctacggcgaa gacgatcagg ttgctttgca gcgcttaatc gaagaaatcc 16560agtctgaaat cgactaccgg ttgtccgata tcgccccgta tgacgacgaa atggcggcaa 16620cactcgccaa agcgtccgtt atcgatcttg aagcgctgga agcgctctcc cgtggagatg 16680acgagccgat cgaagaggac aaacgcgaga aaaccgagcg agtcggtgct gaacaccaga 16740cgatgcgctt caaggtgaca tttgatgcat cagatcgcgt cgccgacacc atcaaaacca 16800tcatcaaaga gcagggaatc aataccggta acgaaatgga gaacgccggg gaagccctgg 16860tgtggctggt cgactactac aaggagcgta tgtaatgacc aaaaactttg aaatcgtcta 16920tcgaaacccg gcagaactca tcccgtatga gatgaacgcc aaaaaacatg acgaacagca 16980gatccgcgac ctggctgccg ccatcaaaaa gcgcggtttt gaccagccga tcacggtcga 17040caagcacgac gtcatcatta ctggccacgg tcgtcgcgag gcggcacttc tggctggtct 17100ggagcgtgtg ccggtcatcg ttcgcgacga cctgagcgaa gaagaagtga aggcaaaacg 17160cctggaagac aaccgcctgg ccagtattga ctacgacgcc atcaaattgc agcaggaact 17220tgaatccctg gtgctgggcg acgttgaggt cttcggtttt gaagagcgcg agctgaacgt 17280gcttgtcggc agcatgaccg aagagatgga aaccggcgct ctggtgctcg atctgggcga 17340agagacggaa cgccagaaag aagagcacac cgagatcagt cgcgaagtgg ccgctgaaga 17400agtccgcgtc atcgacgtat tgggctttaa aacgctccct gctggctctg ccattgtggt 17460tggggatttg cttgcccaca tggaggaaat cacgggagag tgcggggtag acgctttcgt 17520ggcgtatgcg gagaaagttt cttctgggga gctggctgca tgagcaaata caccatcaac 17580gtatcgtttc agacccgcgt gaataaaacc atgcgcacgc tggagattgc cgaatcgttc 17640ggtcttggcc tggacgaaaa agagtggacg ctttacgaca atctggagct ggaagtgaag 17700cagggcgatg tggtgtacat caccggtcag tccggttccg gcaaatccgt tgtgctgcgc 17760gagctgcaac gccagatgaa ggatgaaggg ctttctgtag cctccatcga tgactttacc 17820ttcaacaatg aggttaacgt catcgaccag ttgggcaaaa ccaccagcga tgcgctgggg 17880ctgctgtcta tggccggatt gaatgacgct tatctctttg tgcgcaaacc atccgaaatg 17940tccgacggcc agaaataccg cctcaagatc gccaagctga ttgagtccgg cgccaaagtc 18000tgggccgccg acgaatttgg tgctgttctc gaccgtgtaa cagctcaggt tgtggcgtcg 18060aacctccagc gtgccgcccg caaggttggt gcgacggtaa tggtggcgac gacccacgaa 18120gacctgaaga acgcgctgcg cccggatatg cagatcacca agcactacaa agaacgcgtg 18180aaggtggaat atgcctgatt tgaagatcgt tgagctgaag ccatcgaaag aggctgacaa 18240caacaacgtt gaagtcatcc gcctgctgga agaagcactc cagcacgcca gagaaggtaa 18300aagccagagt ctggcgttgc tgatgatcaa caacgacggc agtgttctgg attgctggca 18360taacggtggg cgtccatacg tcatggttgg ggcgatggaa tcgcttcgcc tggacttcat 18420caatgccaat atcgagcgca ggtgatcgac atgacagaca tcatcatcaa acgctaccgc 18480ccggaagagt tcccgcgtca tctggacttt ctggagcgta tgaccgttac caaaggcacg 18540gttgaagact ggcacgcgct gaagtcgctg cactataaga cggatggtaa gccgttcgcg 18600ccaacgtatt atcgctgcga actggacgac cggctggtgg gcgtcgtggt tatggcttac 18660ccgaaactgc tgctggcgcc tcgccatcgc atgtttccta agctgaagcc aaccaccaat 18720accaccgtgg ccaaccagta ttgggggcgg tacgtgaaca acaactttgc ggtgattagc 18780cgttccgttg tggacactca gtaccgcggc gtcggcgtct cctatcgaat gattaacctg 18840gttagcagga tgcacgaccg gccaatcatc gagatccagt cgtcgatgag caaatacaac 18900ccgtttgcca tgaaagcagg gttccagttc atccgtccgg agcgtccgaa gagctatgag 18960agtgcgttgc gcgtcttcca gcgtcatttc cgttccgacc ctggcgacaa cgaagcgatc 19020gtcaaagagc tgttcgccat gagtgagtct cgccgtcgtc gtgcgctgcg tgatctggtc 19080gctgactacc acaagaacag ttccctggcc aaagccgggc gtaatcgtgg cacgacgatt 19140caggacattg ccgacagcct ggtggacgag gccagcattg tgaagctgct caaggatatt 19200cacaacctga gcttcacgtc tccgctgtat ggcgtgtacc gaaacccgga ctttggccgt 19260cagttgcctg acacgctgcc actgctggca ttcgacaaac agcctttgaa caaacctctt 19320gaaattgcat taccggcata aggatttgcc atgacgttga ccgacaaaca aaaagacatc 19380atcaaaacca tcaatttagg ccatgagcgt gggcatctgc tcgatctgga cgagctgctt 19440gaagtgctgc cgtacaaaac gaccaaacag agtatgcagt tctctatccg cgcactggtg 19500aaaaaggggc tggtggagaa aggaatgtgc cgccagcgcg gtgattctgg ctaccaccgt 19560cgcacgctgg ggctgaccac gttaggtcgt gccagagcca aattactggt gatgtaagtc 19620ggtctgggag ccagtttgag agcctgcttc cgtatatata aatactaagt gacttattaa 19680atatatacgg aagcaggttc tgaatactcc ccagcccggt tttaaacacc cagaaaacaa 19740attggttagg catagaatta aacaagttgt ttaggagcgc aaggatgcgc tctgagtgtt 19800ttagagggat ctatgactgt agaaaaagac gagagcaaaa ctcgcctgac gccagctgag 19860tgggcagaag ccgaagcgaa gtggacttcc ggtgaataca cactctccaa gctggaggaa 19920gagtacggca ttcgtcgtga aacgctctcc agacatttca aaaagcgtgg attagagaaa 19980ggcgcggact ccgtggggaa gatggtgcgc gagtcgctca aatccgacgc agagctgcgt 20040gcgaaggcgc gtgcagagaa aatcgaagag cgccggactc gttacgacga ttgggcgttc 20100gcactcggtc ggatggtgat gcatgaggtg gccacagcca agaaggacgg caagccactc 20160gcaaccatcg aagatgacct gaagagtctc cagcgtgcca gcggcacact cgctaagtgc 20220ttcgaagtat cgtcgaaagc gctcggtatg gatcgcgccg aaaacgagga cgacgaaatc 20280ccgaatctgg tatttggcga acttacgcct tcccaggtgg cgcagctgcg taaggaagat 20340gatgagccgg atctgattga tgacgatctg cttgagtcac tcgaagagga agcactgagc 20400gaagctgagg gcgattctga cgcgtctggt gatgaaagtg atgggagcgt ctaactatgg 20460ccatcccgtc gtctctgagt ctcgtacagc tgcattctgg gcagatgcaa gtcttccagt 20520cgccacatcg tttcaaagta gtgtgtgcgg gtcgacgctg gggtaaatcc cggttgtcga 20580tttccaccat cattcgcgcg gcagccaaag agaagaagca acgtgtctgg tacgtcgcac 20640cgacgtacca gatggctcgc cagatcttgt gggatgacct gcaggaagtt ctgccgcgta 20700aatgggttcg taagaaaaac gacaccacga tgaccatcgt gctgaagaac ggctctgaaa 20760tcgcgctgaa aggtgcggat aagcccgata cgcttcgtgg tgtggcactg cactttgtgg 20820tgctcgatga atttcaggat atgaagccgg atacctggta caaggtactt cgtccgacac 20880tgtcctcaac ccgtggcggt gcgctgatca tcggtacgcc aaaaggcttc tccgagttcc 20940acaagctgtg gactatcggt cagaacaaag atttgcaacg caaagggcag tggaagagct 21000ggcagttcgt tacggccgat tctccgttcg taccgagcgc ggaaatcgaa gcggcgaaga 21060acgatatgga ccctaaatcg ttcgcacagg aatacctggc cagcttcgaa aacatgtccg 21120gacgcgttta ctacccgttc gaccgcaatg tgcatgtgaa gccactccag ttcaatccga 21180aactgccgat ctgggttggt caggacttca acatcgaccc tatgtcatcg gtcatcctgc 21240agccgcagcc aaatggtgag ttgtgggccg tggacgaggt tgtgctgttc tcttccaaca 21300cggctgaagt gtgtgatgag ctggagcgcc gtttctggcg ctggaagtct caggtcacta 21360tcttccctga cccggctggt gcgtatcgcc agcacgcacg cggcgaatct gacgtcgata 21420tattcaagga aaaaggtttc ctccgagtcg attatccgaa gaagcacccg cctatcgcag 21480accgtgtgaa cgccgtgaac cggatgttga tgagtgcctc gggcgaaacc cggttgtaca 21540tcgatccgaa gtgcaaacat ctcatcgact cgctggagaa ggtgatctac aagccaggct 21600cacgcgatat ggataagact ggcggcatcg aacacagtgc ggatgcgttg ggttatccgg 21660ttcatcgtag gtatccggtg aaaaatcgtg ttattcttgg tggatctaga taagtaagtg 21720cctacctaaa tatggaaaag aaacaaatgg aattgactga taagcaaatc aaggaccttg 21780tggcacgacg ccacccggaa tatgagaaga aaaaagaaca ttgggatttc ctcgccagca 21840cctacgctgg cgggcgtgcc tggttcaacg acaatatctt tcgttacttc aaagagggcg 21900atcaggagtt caaagagcgt ctggaacgtg cctatcgctt caaccacact cgtgaagtgg 21960taaacctcat caacaaatac ctcttcaaag aggtcattca tcgcaacacg gatgaagcac 22020cggagcagat ccgcaatttc tggaagcgag ccacgcgcca gaacgcctcc atcgatgcgt 22080ttatggcggc tatcgatctg caatcatcca tctatggtcg catctgggtt gttgtggaca 22140gcaccatgaa cgtcgatgtt gagtctgttg cagacgagaa gaaaaatgat gcgcgcgcct 22200acgcttactg gatttcgccg cagcagctgc ttgatgttgc ctgggacgaa gacggcaata 22260tgttgtgggc gctgattgtt gaaatcgcgc gcgacgacga agatccgttc acgtcaaccg 22320ggcaggaata ccagcgttac cgtctgtgga cgcaaaacga gtggtatctg ttccgtgaag 22380aagtgaagaa aggttccgga aatagcggtc gtcgtcaggc caaagtcgtt ctggaggata 22440gcggcgagca taatctgggc gtggtgccgg tgttcccggt ggattgcatt ggtgaaagcg 22500agtctccgta tttcagcccg tcgttgattg atgatatcgc ctatcttgac cgcgccgtgg 22560ccaactacct gtcgaacctt gatgcgatta ttcaggatca gacattcagc cagttggcga 22620tcccggttca gtcattgctg ccgggcgatg aaaaccacac caaagtgctc gaaatgggga 22680caaaacgcgt cttcaccttc gactctgaga gcggtaatca gccattctat ctgtctccag 22740acccgaaaca ggcccagatg atcatcacca cgattaagac ggtgattaac gagatctacc 22800actccgttgg tgtggcaggt gagcgaacca agcaggataa cgcacagggc atcgataact 22860cttcgggcgc agcgaagatg tacgacttcc agcgcgttaa cagtctgctg gtgacaaaag 22920cagaacgcct cgaaagggca gagcgccaga tgatgcaact ggcagcgaaa tggatgggtg 22980tcgaactgga tgaagaccac tctctgatcg cgtacccgga aagttttgac attcgcggtc 23040tgactgacga gtttgccgtt gctgagaaac tgtcgctgct ccaggcgcct gattctgttc 23100gtcgtcatca gatggaaatg ctcatcgaga aggtcttccc gaacatttct gaggcgatgc 23160aaaaggaatt tcaaaaagat ctcttgaaat ttcctccaaa aaatgatctt aacacccttg 23220aaaataagtc agtacttact tatgatcgag atatatccca agaaagcggg caagatcaac 23280cccgagggaa tggggactca tctactcaag agaccgagtg ataagtaacg aaaaggaatt 23340tctatgaatc tgtggcaaat gcttatggcc cgtcgtggcc tgatggatgc agctgaagcg 23400catgagcgcg gaggcgctgg tggcggtgct cctgctggag acaacgagca gggcaatcaa 23460gacccaggta aacagggcga gcaaaaagag caaccgaagg gtgacgacga cgagtatgcc 23520ggtatgactc aggaagagtt gctggcagaa ctgcgtaagt ccaagaaagc tggtgctgac 23580ctgctgaaag agaacatgaa gcgcaaggag aaagagcgca cattggccga tcagctggct 23640cagtacggtg atattgaccc ggcgcgtgct cgccagcttc tcgaagctga acaagccgca 23700gaaaccgcac gccgggaggc ggagcaggct gaactggaac gccgtggtga gttcgatgct 23760gtgaaaaagc agatgatcga agcgcaccca ccaggctgaa ctggcacagc gcgacgaacg 23820ctactccgct ctggagagcg agaacgccga actgaaggct caactggtcg aaatgactgt 23880tggcgcttcc ttcagcggct ctgccttcct gcgtgacaaa gttctgatga ctccggctaa 23940ggctcgcgtt atctacggct ctcatttcga agtgggtgaa gacggtagtg ttgtgggctt 24000tgataagcca gccggtcaga aagagcgtgc agttctggtt gacggtgaag gcaaaccgtt 24060accgttcgaa tccgcgattg aacgcattct gcgtgcagat ccggaagctg acgcactgtt 24120gcgcagcgaa gccaagcagg gtgctggttc caatagcaaa ccgacccaca aagtaaacca 24180gccgaagagc aagtcgacta tggataagtt gacctccggt ctggggaaaa tcggactcaa 24240gtaacatctt aaatcatagg gaaatgaaag atgccattac tgcgtgatga agctgaaaag 24300ctatctaaca acgaacttga gcagggtgtg atcgagacca tcatcgatcg cgatgacctg 24360ttcgctgttc tgcctttcat gaagattaac tctaaggcat acctctacaa ccgcgaagct 24420accctgagcg aagcaacctt cattgatgtg aacgacacca tcaccgaagg cgctgcaacc 24480ttcaccgaac acgttgcgaa gctgcgtatc ctggcaggcg acgtagacgt cgacaaattc 24540ctggcgacca ctatgtccga caccaacaac cagctggcaa tccaggttcg tcagaaggtg 24600aaaggtctgg cccgcgcatt ccgccgcaac ctgattctgg gcgactccac caccaacacc 24660aaagctttcg acggtattcc gaagctgatg cacgacgatc agaagatcga catcgaaggc 24720gcttccatga ccttctccat gttcgacgag ctggtcgacg cggtgaaaga tctgggcgca 24780gactgcatca tgatgcgttc cgagcacctg cgtgcttatc gcgctctgct gcgtaccgtt 24840agcctcggcc cgtcagaaat catgatggaa aacttcggcc gcccgatgct gtgccacaac 24900ggtgtaccgt tcatcgtgaa cgacttcatc ccgactgatg ctggcaaagc aagcatctac 24960tgcctgcacc tgtccgaaga gaacggtgtg accggtctgt atggcggcga aaacgccggt 25020atcgttgttg agaacatcgg tactgttcag aacaaagacg caacccgtac ccgcgttaag 25080tggtactgct ctctggcgaa caagcacgat aaggctatcg ccgcgctgac caacgtaaaa 25140atttgatcag tatcgtaggt aagtaattat ctaccattta agggtgggct atacgcccac 25200cctttttgta ggagcgagaa atgccagaac aaaagatgaa gatcacggaa gaggcgtttt 25260cggatttcac ggggcatatg tgccgtgccg gattcaccaa ttccatctcc aacgagcctc 25320tgagcgagcg ccagcagaat catcttgcgg cttgcttccg ggcaattccg ttcactcagt 25380ctgtcaccat tacaccgact gcgccgtccg tattggtggg caaaaccgtt caacttagtg 25440caggtatcac catgagcaaa agtgcagatt cattcacctg gacgtcagac aatgaccggg 25500tcgccacagt cagcggtacg ggtctggtta ctggcgtgac tccgggcaaa gtgaagatca 25560ccgccaccga taagcagact cagctttctg cctcagtcga agtgatcgtt aagcctgtca 25620gcgtggagtc cgtaaccgta acgcctgact ctacctccgt tgagaagggg aaatcggtca 25680agttgcgtgt tgatgtacaa ccgtcaaatg caaccaataa aaaagtcacc tggacttcca 25740aaaatagcga caaagcgact gttgaccaga acggtaacgt agctggcgta gccgttggta 25800cggcaactat tgaagtggtt tcgcaggatg gcagccacaa agctactgcg actgtggaag 25860tgactgcagc accggctgcg taaccaatac atgggcggca tagccgccca ttaagtgaga 25920agaagaatga aaccagcaaa aattcgttta ttggagcctc aatttttggg gtacacgggc 25980attctctgcg gtatccagtt tgtcgacggc atctcggttg ccgaactgcg attcatcgat 26040cagcagcgga tttgtgcctc catgcgtgcc actaccgttg aaggcaaaaa tgtatctcct 26100tctgccgcgt acagcagccg caatgatttg actgcggacg acattgtcga gacggcggcc 26160ccggatattg tgccaatgaa acgtggtaca gctgaagtgg aagccaaacc ggtacagcgc 26220tttactcgtg aagaactgga gtcgattgcg gactgtgaag gtattgcggg tctgcgtcag 26280atcggcaacc agattggcgt gaaagccaaa ggcatcgttg aaatgatcga aggcatcctg 26340aaagcacagg gcggtgagta atggcgcaga tcgacacgta ccgtagcggg gaagctgttt 26400ccctgtcgtt tgcatttaac gttctggata ttgagtcggc cacgtatacc gtcagagatg 26460gcgctggcgc gatcatcgtc gataacgaac ctctcgatat tactgagggg cagatgtcca 26520ttccggttgt cgtgtcggcc gaacacaacc tgctttcaga taaagagcgc gatctgcgac 26580acgtcattgt caaagcggtg gcatccgggc tgacgcatga agagcgcaag atgtacgttt 26640tgctgaatag cttcgagctg tcaattccag gccagtcatt cgcaacggtc gcagacgccc 26700agatgcaagc tatcgatatg ctgaacggcg acaccctgtt agctgatggc gaagggctga 26760tgcgcaaacg tctcattgag gccaccagac gcgtcaaaac gctgccgttc tcaatccgca 26820aagtcctgcg tatcgacttc gaccggtacg atcgcccgca aaacatgctg aacgtctatg 26880acattccgtg gggcgctgac ggggcatatc gtcacgatct gatcgattgg gagcagatga 26940cgccggagaa attcgacgag ttcccggact acttcaaaca ggcattaatg ctggccgtgg 27000ttaatgaagc ctgcgaaatc gctaacggta atgacgtagc ggcagcccgc gaggacggca 27060ttctgtctga gtccattggc gaaacgacca atatgtaccg taccggcaaa gcggcaaacg 27120tgcaggtggc tcgcagtacc tggcgactgc tggtcagtta catcaataac cgcatgattg 27180ttcgccgtgc gtaacgccag tcgcattatt tacttctggt cgaaaggctc aagacgagca 27240atcgcgcctt cgcctggtaa tgagtgcggc tgccaaccac agggagagtg catgaacatt 27300tcatggcaag cagagatagc gatttaccgt ctgggttcga agaacgtcta cggtgaagcg 27360caattgcagt tcgtcagaaa gacgaacgtc ggcgtcgtta agtttgagca aagtaacgag 27420aagtcatcgg tacgtgctga cagctccggc agtcgcggta aagcgaatct ggaattgttc 27480gatgctgttc tggtgatccc acttgaggcc gcagtacagc ttgatgacgt tctcattctg 27540gaggggcaaa agctgaaggt atccagcgtg catcgtcgct ggggactgcg tgggcgccct 27600gggcatctgg aagtaggggc aaacatatgg gtctgaagta cgacgcgcat cattttaagc 27660gtgctggcga caggctcaat aacagccaga aagcctttaa gcgttatctc atccgtgaca 27720tggagaagct ggcgcgtctg gttgagcgtc tggcgcgggc aatggccccg ctggagactg 27780gctcactcga aagcgcgatc ttcgcgagag ttgtcaaaga aggctatacc gggctgcgta 27840ttgagttatc ggtatctgga gccaaaccac gcgaaggtca tccgggcgtt gaggttggcg 27900attatgcgga gtacatggag ttgggcaagt atcgtctcgg ctatctatcc cgcatgaaga 27960gcgtcaccaa cccgccagtt gctggagtga agccacgagt tgggcctttg ttccttgaga 28020gagccgtgca gatcagtgag aaacagttca ctcagacgat agcagaagcg gcaaggaaag 28080caggttttac gagaggttga tgtgtttatt gaagcatttg cgagcctgat gcagaaggcg 28140aagatcggta cagtcggcac tgacattttc tgtcactaca tgccagccaa tgtgaagtcc 28200ggtgttctgt tggttactcc caatacgggg atcaccattg accatgagtt aaaaggcttc 28260tatcacgact ctttcaccgt catcgtgcgt aatgcgacga ttacaaagac ggtggcgaaa 28320gccaataaga tcatggacat gttcccggtc gaagaaaccg tgtcagatgg cgtttacttc 28380cggttggttc gaccaatgtc gatgccgatt acttatccca aaaatgaagg ttcgttgatt 28440gaagcgggta tcccgattga atttgcgggc tatttgttga attaataaaa taggtaagta 28500tatacttatc attaacacca tgaaggtgct gatttaacgg aaaaaggagt tttccaaaaa 28560tgtccaatac ccatgtaaaa aacatcaaac ttggcgcctg caaagtgtcg tttggtggcg 28620ttgatctggg ttacaccaaa ggcggtgttc aggttgaagt tgcgaccgaa actctgaaag 28680tcaccgtaga ccagctgggg cagaccacca tctccgagct ggtacagggt cgtaacatca 28740ctatcactgc gccgctggcc gagtctgtgt tgcagaatat ggtcgatctg atgccgggtt 28800ctaccctgag cgaagaagag aactctgtga ccatcacttc cgcacagggc gtcaacctga 28860tcgacgtagc caaagagctg gttctgaccc cgcaagatac caccgactat gtcctgacca 28920tcccgaaagc tgcaaccgca ggtaacttca ccatgaccta ccagtctgat gatgttcgcg 28980tgttctccgt tcagttcacc gcttacccgg atgacgacgg cgtgctgggg aaaatgagcg 29040gcccaaagcc ggttaaaacc gtctctatct ctccggaatc tccggaagtt aaagccggtg 29100agaccgtgca actgactgcc cagatcaccc ctgcagatgc cggcgacaaa accggtgtgt 29160gggaatccga caatcaggag aaagcaaccg ttgaccagac tggtctggtt cgcggagtag 29220ctgaaggttc ggcaaatatc tcctttacca gcaatagcgg cggcaagaaa gcgaccaaag 29280cagtaacggt taattctgcc caataatcgc gacgtaatta agcagaggct caggaagagc 29340ctctctttta aaaggacttt aaccaatgac caaattactc gatctcgact ccattctgcc 29400tccgaagaaa agcatcaagt tcggcggtca ggaatatccc atcgttgaaa tgactgttgg 29460cctgttcgtc tctatcaagc agatggaagg caaagacctc cagaatatgt cgcctgttga 29520gcaggttact gcttacgccg acctggttcg caaggtcatc ccatccgtgc cggacgctgt 29580actggaaaaa ctgactgttc cgcagctcca gcagatcttc accttcgcta tggaagtgat 29640tgatgaagag aacgaaaaag cggctggtga aggggcaaag taatttcccg cgatgaatcc 29700ggggtaaaga ccgtatcgat agatctcgga ttctatttca gtcgtgtagt tgctcactac 29760gccgtgtcgc cattagagct gctgggcgtc cctctaacga tgttctggat gctcagtcgc 29820aacatcgacc gtctgcgtgc ggaagaggat gtccgcaacc tgcaagtcgc tcgcgctgcc 29880caggcagatg gcgagggcgt aaaggcgttc atggagggtt tgcaactcag gattggaaga 29940ccagtcgtaa ccgataaagt ctacgatcca cgcaaggata aggcagaccc tgacgccaaa 30000gagcaactga tgcaaatttt tggcagagga tgacaaggga atgtcacaaa acgtagagtt 30060tatcctgtcg ctggaagaca agcagtttac agcgtcaatc gaccgggcgg gtaagctact 30120taccagattc ggggagcagg ccacaaagcc agctcagaaa attaacaatc tggaacgctc 30180gttgggttcg gtctcccgca tcatcggcgt tctggagtcc aagctcgatg tcacggcaga 30240aaaactacag gatgtagctg ccggcttcga gcttgtgtct gatgtttcgc gcaagacgcg 30300aggcagcatt accagcctca actcaggtct caaaaccctg attgagcgcg tcgacacaac 30360cacctcatcc gttaataagc tcaccacatc gctgcgcaag gttcagtctg aactcaatga 30420gttttccgat tgggcaacgt tcgctggcaa gagcgccagc cgcttcggta cggaggtaaa 30480agaagcctct gcatccgtga gcggcatgaa tacgcgtctt aacaccacga caaagcgtct 30540cagtaattgg ggtgtcacaa cgagccaggc tgctgagggg ctgaagaaag tccgcgatca 30600gatggatgcg gtgattggcc gccagcaact gatcagcaag ccggtacgtg tacgcaccag 30660tagctacggt gatggcggtg gtaatggcgg tggaggtcga cacagcggtt cctatggcca 30720cggtgggcgt agtgctgaaa gtggtgtgtt ctctggtctg cgcggcaaca ttttcttgct 30780tggcgagatt ggtgatgcgg caagaacggt aactgacatc ctgttcggct ggcagaagcc 30840aatcatcgaa gccgcctccg aaatggagcg tatgcgtgtc atgttgcgcg ggctgaacaa 30900ggataaggcc aaccctggaa aagccgccgc agaggatatg cagtacatcg tggatatggc 30960gcaaaacgcc ccgtttgcga tgcaggcgct gaccgattcc ttcgttaaat ttcgctcggc 31020aggtctcgat cctactgacg gatcgctgaa agcactggtg gactccgttg cacgcttcgg 31080cggcgatagc gaactgctta aacgtgctgc cgtggctgtc cagcagatgt ccggtaaggg 31140cgtcgtgtcg atggaagaac tgcgtcagca attaggtgaa gccgttccta acgcgatgaa 31200ggcgatggca gacgctgccg gtatcactat gggggaactg accaaagcag tctccaccgg 31260tactgtggaa gcgaaacagg cgctatcgct gatgttcgtt ggtctgcgtg cggagaacga 31320aaacgccgcc aaagacatga tgcaaaccta caccggtgcg ctggcgcaac tgcaaacctc 31380ctttacgctg tttgccgatc gtgtcggtca ggcgggctat ctggattctc tcacaaaggg 31440gatgaaagag cttgcttcca tcatgaacag cgctgaaggc atttcgttcg ctaactcgct 31500gggttccgga ctctccacgg cgatcgacgg cttacgcgag ctggcgcagt ggctggctaa 31560aaaccaggag ctggttatca gcctgggcaa agtcgttgcc gcaatggtgg cgttcaagct 31620gatgcgtgcc gggattgcag gcgtgattgg cactgccggg caaatggtta acacatttac 31680caagatgtcg accgttctcc aggctccgtt caatctgggc gcgacagctg tcacccgctt 31740taatcgtgcg gctcgtatgg ggctggcccc aatcccatcg ctgatcttcg ctatccgtgg 31800cgcgattacg gggcttcagg gcgcgtttgc tgggctaact gcgttcattg cagcaaaccc 31860aattggtgca gcgttcaccg tagctaccgt ggctgtggcc ggtcttatca cgtatatgac 31920catgctccgc agcgaaacgt ccaaagtcgt ggacgagatc cgcaaaatcc cggaggcgat 31980gacggcggcc aagcgtgcgc agatggcagc gcgtgcagcc gagcttgaaa agcagatcca 32040gcgagaccag caggcgctta aaactggcga aagcgtgaac tactactcca cagcggctgg 32100ccctgtcgcc gtgaaggagt ccaaagaggt tgttgaggct cgcctgaaga aaaatcagga 32160agagtacgaa agaacaaccg gcacgatggc gctgggtgat ggcgcagtgg ccaaacgcct 32220ggcaaaagaa gctgccgaat cccagattga gaaaatccgg gcagataacc aggtcttctc 32280tgcgacgttt gtgaaagccc gtcaggaggc tctggataag atccagaaaa tcaacgacga 32340caaatcgctt tcggacgacg agaagaacaa gctgcttgca ccgcttcgcg agaaggtgaa 32400caagtcctat ctggaaccgg cacagaagct ggtctacgac ctgtcttctc gtaaaaacgc 32460caccgagaag caaattgccc acttcagcga catgctggag aaagcgaaga aagaggggaa 32520caccgagcag gttcagaagc tgcagggcag catccgcggg tatcaggagc atcttgaagc 32580tgtagctcag gagctgactc aggcggagtt cgagcgtgat agcgcggcga aaaccggtaa 32640gggcgtaatg tccaaccagg gcaccgttct ggggttgggg acgaccgata aggcggctca 32700gaaagcactg gcgcagtata tgcggaacca gatggactcc gcgacctacc agcgcacact 32760gccagacggt acggcgatgc tggacttcga aggcaagccg atcatcgggc cgaaacagct 32820caaaacccag ctgaacctgc agaaagcctc cagcgccagc tctctggaga aaatgagcga 32880cgaggaacgt gctgccgcga tcgccgcact gaccaaagcg cgtgagcagg acgcagcagc 32940agcggaaaaa gccgggcagc gtactgccaa tgcttctcag cgtgccgcga ggaaggaaga 33000aaatgcgcag cgtaagctgg cggccggcta ccagaaagcc ctggataaag ccgatcagct 33060tatggggcag atgggggaaa gctcaaaagc gaccgtgtcg tttgaccagt ctctccgcga 33120taccaccaaa tcgctgaccg aactggccaa cgccgtaccg aatgagttca tcactcagga 33180gatgatcgac aaagccaagt cacgcctcgc tgacctggcg aacgcgagcg acgactatcg 33240cgagatgttc aaccgccgca acgtcgagca gatgatctcc acctgggcgc cggaatccga 33300ttccatcatc agcgcgggtt acaagccgtc tcgtgaagag aaggtggccg atttcaacga 33360cacctacaac cgtaatctga aagcgttgat ggatttgcgt gaccaggctt ctgatccgaa 33420aatcgtggcg ctctacacca agcagattaa ccagctggtg gcggcaggca acaccgcgct 33480catcaaagag acgggtacgg cgacacagaa gctggcgctt gagtacgaga atctggccga 33540acagctggaa aacagctgga gcaacctgtt cagcaacatg acggatacgc tgaccgactt 33600cgtcatgaag gggaaactgg acttctccag cctggcagag tccattctcc gcgacatcac 33660caacatggtt gtgaagacgc agatcactct acctctcatg aacatgctgg ggatggggac 33720gacggcagcg ggcagctctc agagtggcaa tctgctttct ggtgtcgcgt ctgcggttgc 33780caaccagggc gtccggatga atgcggtcaa cggcgataag agcgtgggtg aggcgacgaa 33840agagacctcc agctcggtct ccggtctggg gcagaccact cagcagacga ccagcgcgat 33900tggttctgca accaacgcga tcggcaactg ggtaaatgga ctgttcacca gtactgaagc 33960caaagacgcg gaaaccaaag cggtgaagac gtccatcttc tccatgcaga accttagctc 34020tgtcacgggg gcgctttctg ccgcgtttgc tatgctgggc gcaaacatgt ccggctctgg 34080caataagtgg ttgagtttcg gcgctaccat tgcctccggg ctggtgtctg cctgggctgg 34140tggtggcttc gataacatcg gatctggttc ctccggctct aactccggat tcaacaatct 34200caccggatcg gcatctgatg gtactggcgg cattccggca atcccgaagt tcgccaaagg 34260cggcattttc gggaaagacg gcgtggttcc gctgcgtgct taccagaaag gtggcatcgc 34320tgactctcca cagctggcgt tatttggcga aggggatatg aacgaagcct acgttcctct 34380tccggatggg cgttccatcc cggtcacgct caacgcagag ggtgttaaag gcggcggcgt 34440tttctcacct gtcagcattg aaatcaacgt caacagcgac ggcagtgtct cggagaacag 34500caattccgaa ggcgcatgga gtcaggctgc gcagcgcatg aaggcgatcg cgcttgaaac 34560catcgctcag gagaagcggc caggcggttc gctcaaccct aactctcaac gtaactaacc 34620acggctgccc cggaaggggc ggtctcacaa ggatgtgaga tggaaagact gacttttaac 34680tggtatcccg actacgagtc ggaaaagacc gtgaagccta acgtgacggt gctgaatttc 34740ggtgacgatt acgaacagcg tcaggcaaaa gggcttaatc gcattaaaga agagtggagc 34800cttactttta cgcgttctta tgacgtcatc aatgccgtcg atgactttct gacggcacgc 34860gcggccgttg agtcgttcta ctggacgaac cctcgaggca aaaagatggt tgtggtgtgc 34920gacagccata ctgtgaagcg ttatcagggc tatctcgttc tcactgcgac cttccgacaa 34980atttatgaag gataatttaa cccactagat aagtaggtgc ttatttacta ttatctatag 35040gcgctgacag gatgttggcg cctctttatt tcaaggaaga aacgatgggt attaaagctg 35100atattcagag cttgtcgccc tccgcgctca ttgagctgtt cgaacttgat atgtcgaaca 35160ccacctctgg gggcaagctg tttttccacg ccggtacaaa cgaactgatg gagccagtcg 35220tttggcaagg tgtgtcctac gaaccgtggc caatcaaggc gtcaggcttt gataagactg 35280gtcagggtac tttgccgcgt ccaaaaatcc aggtctccaa ctttgccgga accgtctccg 35340ctgaagtcca ggcaaatgac tatctggtgg gttgtcgcat catccgcaag atgacgctgg 35400cgcgttttct cgacgcggcc aacttcaaag acggaaatcc gaccgcagat ccgaatcagc 35460attttcccga tgagatgtgg ttcgtcgagc agaagactct tgaaacccat gaggttgtcg 35520agtttgagct gtcgagcgtg ttcgatctga tgggcgtgca actgccgtac cgccagatca 35580tcaaaaacac ctgcccgtgg aaataccgcg gcccagagtg cggctatacc ggcccctatt 35640tcgacaaaaa caaccagcaa accaccatgt caggcgcgga ttactgcacg aaacgctacg 35700actcatgcaa cgcacgccgt aactactttg ccaatggcgt aatccacttt ggcggattca 35760ttggagcaac acgttatggg taatagagct ttccctgagc ttgggtcgga cattatgcag 35820gaaatctatc tgacagccat caaacgctac ccgaacgaag cgtgtggctt tctggtgcgt 35880actactggcg agaaatatcg cttcatggaa gcccggaacg tgtcggagaa cccggaaaac 35940acgtttgtga tgcacgctga cgacattatc gcagcggaag atgcgggaga cgtggttgcc 36000atctggcact cccacactga cgaatcagct gatgcgtcag atgccgaccg cgccggatgc 36060gaggcaacgg aagttccgtg gctgattctg gctgttcgga agaacgtcga gggcgatgcg 36120ccatttcact tcagtgagat gaatgtgatc accccagacg gctttgagat gccttatctg 36180ggtcgaccct atgtgttcgg tgtcttcgac tgctggatgc tgtgccgcga ctacctgaag 36240cgtgagttca acgtcgagct gaatccgaac ccgcacctgc atattccatc gtggtacacg 36300ggcgataccg acattctcga tcagaactac cgcaatgaag ggcttgttcg tctggcgccg 36360gggacggaac cccagcgtgg tgacgtcttc ttcattcagt acggaaagat gcctgaccac 36420tgcgcggtgt acataggaga cggaatgatc ctgcaccacc agatcgaccg tctgagctgt 36480cgcgcttatt acggtggcat gtaccagaaa cacacgacgc accacctgcg tcacagagac 36540ttactcaagg gagatgagac gtgtctgagt tagttcatgt gcaacttggt ggcccgatgg 36600ccagacattt cggccgccac tggcatttaa aagtgcgcaa caccaaacag gcgttggatc 36660tggtcgaagc caatcgtccg ggcttaaaag cctggatgaa gcgcaacatg aagacctacg 36720acaagtatca catccagatc accaataagc aggggcataa gtggtcggtt gatgagagcg 36780agtttcaaat gatggggcag tccgacaaca tcgcgaagat ccgcatcacg ccggttcctc 36840gcggtagcgg cggtaaggct tttggttggt ttcagacagt cgtaggggca ctcgtcatgg 36900ttgcctcatt ctggtttccc gcgctggccc cgctcggctt gtcgctaatg atggggggta 36960tttcgcagtt aatttcgccc caggcgacca atgacagtgt aaggcaggca gataactcga 37020actcgtttta tttcgacgga ccacaaaaca ccactaacca gggcaacccg gttcagctca 37080tttatggtga ggaaattctg gtcggctcac aggtagtaag ttcttcgatc accatcgacc 37140agctttagta agaagggaaa tttttgaaca tggaacagtt caagaagaag agactgcctc 37200tcctgattgc aggtgctggc ggtaagaaaa gcagtggctc cagccgcaca ccggttgaag 37260ccgacgatac cgtaaactcg cgtgctatgg cgtccatcct cgacctgctc ggggaaggtg 37320tcattggcgg gctggtggat ggtgcaaagt cgatcttcgt tgatgatctg ccaatcctta 37380acgaagacgg gtcttcaaac tttagcggta tcacctggga cttccgcgat ggttcacaag 37440accagacgcc gatggctggg ttcgatttcg ttgaaacgcc gaagtcagtc aacatccagt 37500tgaaaagaat gcacgacgtt acgattgcca tcgataacga tgaggcagac cgtgtccgcg 37560tcattctgaa gttcccgtct ctgcgtagca tcgacaaaaa gaccggtgat accaacggta 37620cgaccgtgaa gtacaaattc cagattgcca atggcgataa tgccttcaag gacgccatcg 37680cagaagggga gagtgcttcc gaaattgcgc tgacggcaaa aaagacaggc gtctactacc 37740gcagctatga gctaaaactg cccaagccag gtcgtgccta caaggttcgc gtgctgcgtc 37800tgaccgatga cagcaatact cagtacatct ttaacgatac gtgggtggac tctatcggtg 37860agatcgtcga tacgccgatg aactatccga actccgcgct ggttggcctc aaggtcaact 37920cagagcagtt cggcagctcg atgccgtctc gttcgtatct ggtgcgtggc ctgaagatcc 37980gcgtaccgtc caactacgat gaacacacaa acacctatat cggcgtatgg gatggcacat 38040tcaagctgtt gtcatcttcc aaccctgcct ggattctctt cgacctgctt accaacgctc 38100gttatggcct ggggcagtac gtttctgagt ccatgattga cctcgggcag atctaccaga 38160ttggtcgcta ctgtgacgaa gaaattgaca atggattcgg gggcaaagag aagcgcttcg 38220ctatcaacac ccagatcact agccgtcagg acgcgtaccg actgattcag gatatcgctg 38280gcgccttccg cggtatggtc ttctgggctg gtggcatggt taacgtcatg caggatagtc 38340cgtcagatcc ggtcatgatg ttcaccaacg cgaacgtcaa agacggcatg ttcagctaca 38400agggatctgc gcgtaaagac cgtccgtcag tagctcttgt gacctacaac aacaaggaag 38460acggctacaa gcagaacatc gagtacgtcg aagaccagga ggcgatgcgt cgttatggcg 38520agcgtaaaac cgaagtggtt gcgttcggct gtacaagccg tggccaggcg catcgtgtcg 38580gtctgtggct gctgtatacc gcacgcatgg agtcggacgt tatcagcttt acggcagggc 38640ttgatgcttc cttccttatg ccgggtgaaa cggtgctgat tcagaacaaa taccgtgctg 38700gtaaacgcaa ctctggccgc attgtggcgt tcacaaagaa cagcatcact ctcgacgcac 38760cggttacgct gaataaagcc ggtagctaca tccggatctt gaatcaggaa ggcgaaatcg 38820ttgagcgcga tattcttgag accggggaag acattaccaa agtgaccttc tccaaagcgc 38880tcaattccgg tgatatgccg gtgatgaatg gcgtctggac gattaccgag ccagatctgg 38940agccaatgcg cgtgcgtgtt atcaacgttg cccagggcga ggctcagggg acgtttaacg 39000ttacggttgt ccagaataat gcatcgaagt acgaagccat cgacaacggt gcgacgctga 39060tccccgagaa caacacagtt ctcgacccga cttattcgaa gccgactaac ctgcaggtga 39120cggaagggac gtatatctcc agtccgggta acctctcaat caagctcgta gccacctggg 39180agggtaagtc tgcggaatat tggatcagct ggcgtcgttc cgatgaaaac aacgtttcta 39240actggcagtc cgcacgcgtt accgaagagc agttcgagat cctcaatatt gctgagaatg 39300gtcaatacga cattcagctc tatgcggttt cgttcagcgg caagaaaacg gacatcatca 39360gcaccgttta tcaggtgaaa ggtacgatga cgccgccagg ctctcctacc tctctgacgg 39420ccgttggtga ctaccgcaac gtgattctga attgggtcaa cccggactca atcgaccttg 39480atcacatcaa cgtgtatgcc tcccagacca acgatctgga aacggcgaag ttggttgcag 39540aggccgccag caccacgttc actcatgccg gtctgggaga tagtgagacc tggtactatt 39600gggttcgcgc ggtgaacaag cgtggcatgt taagtccgcc gaactccaat ctgggtacgg 39660aagcgatgac gcgagacgtc ctctcgttcc ttaccgggaa gatcacctct tccgagctgg 39720ggcaggagct gctggaggaa atcgacgcta aagcctctca ggatgcggtc gacgccatca 39780acaaacagat ggaagagagt ctgaaagagc ttgatcagtc cgttgccgat ctggacagca 39840aactggaaga caccagcggt cggcttgagc aggtgcagaa cgacctcaaa aatgaagtct 39900ctggcacgct ggacaaggtc aacgacgcgc tgcaacaagt tgaggactct aatgcggctc 39960tggtcgagtt gcaggaaacc gtttccgagc agggcaaagc catagctggc gctgtggaag 40020cggcgcacgc tgcgctcgac aacgcctccg cgctgattgc tgaagagcgt gaagcccgtg 40080tcgaaggtga taaggcaaat gccaaacaga ttgaggcaat gaaatcctcc gtcgatgaca 40140gtgttgccgc cgtcgaagag atgaaaaaga ccgttgccga agtcgaacgc gccagcgcgg 40200aagcgtcgac caatatcgag gctctggcca aaaccaatat tgacctcgct ctgcgtcagg 40260atgaagacca gcacaagcag atggtcaata atgcgaagat cgcaaccact cagaagacgt 40320ttgccgacga tatgtctgca atggcctcaa aagtggaaga aatccgcgca gaaattggtg 40380aggacatccg ggcgtcgatt ctggaagaga caacggctcg cgtagaggct gacaagacaa 40440ttgcgacgca tatctccaag ctggaagccc agctcaacga cgatatttca gcggcaatcg 40500tttccgagca agaggcgcgt gcgactgcgg atgaaacgct ttctcgtcag atcaccacgt 40560tgcaggcgaa agttgaaggt gatatcagcg ctgcacttac tgaagagcag attgcccgag 40620ccacagcgga tgaggcgcta tcgaagcaaa ttacccaact gaaggcacag aatggtgagg 40680atatcaaagc cgccgttgca gaagagaccc aggctcgaac cgatgcagat ggtgctctgg 40740cttcgcagat cagctcgctg aaggctcaga cggcagagga catcaaggcc gctgtcgaca 40800cagagacgaa agcgcgtacc gatgccgact ctgctctggc cgggcagatc accaatctcc 40860aggctcagac cggcaaagat atcaacgctg ctatcacatc cgaagccacc gcgcgtgcaa 40920acgctgacgg tgctctcggt aagagaattg atacggttaa ggctgaagtt gatggcaact 40980cggctctcat tcagcagcaa gcgaaggcga ttgccgatac cgataagaag gtttctgctg 41040cctggacgct gaagatggaa acatctacca gcggcgggca gaagtacgtt gcaggtatcg 41100cgctgggtat cgacagtacc ggtttatcac aatttttggt gcaggcagac cgttttggcc 41160tggtcaactc cgtaaacggg aagatcacta cgccatttgt catcgaaaac agcgtggcgt 41220atatgaacgg cgcttatatc aaagacggca caattacgaa cgccaaaatt ggtaatgtca 41280ttcagtcgaa cgattacgcc gcaggcagta gaggctggat tatccccaaa gatggtagcc 41340ctgagttcaa caacggtacg ttcaggggaa atattgctgc aaactccggc acgctgaata 41400acgtcaccat cgcgcagaac tgccagattc tggggaaact gcacgcgaac cagattgatg 41460gcgatattgt taaagcctac atggttaatg gcagcagtat ttatattgca cctcaaacat 41520tcgccagaat tatctatgtg gtaaatggtt actactataa caagccatcg gaggatatta 41580acacctactc atggtcaaga attaccgagt atacggtaaa tggagtgaag cagcagatat 41640atggaatgag agaaggctct aaaaatcaat ctggcttgtt tggttattac aatttgccgg 41700caggtcagtc tgcgactgtt gatgtttata cctggcatag acagcgtaaa tacgatcacc 41760gcgtgaatga accttatctc attctggtgt ttaaggctta aaaatggaga aggatattat 41820ggacgaaacc cctgtcatct ctgctgtaag gaacgcagtg cgcagtctta gtggcggtat 41880ctcttgtgag attcaatttg atggccttgt tatggacgat ggtgtaaccc ctttgttttt 41940gccatacacg ttgtcggaaa gtgacacatc acctttggcc aagaaaatac tggaggctct 42000ggagtcggaa tccagtggtg gtatcgctcc gtaccccgaa caaggcgagt acatcgaaag 42060attgaaagtt gagaaactgg cagtaattaa tgactggcgt cttcgtcagg agtgtcgcac 42120cgtttatttc gaatggaacg gccaccgctg ggacgcgaat gacatttcca aagaacgttt 42180ggatatctcg cttaaagcag cggaaagcgg tctccctgac aatttcttct ggaccgatgc 42240ggataataac gatgttccgg taacgcagga gcaactgaag gaattaggga tgagaatgac 42300ccagacgttg ttcgaccgca agttcggcgc ccacgaacga cagagggtga tgaaaaaaga 42360cattctggag atgtgtgatc ctgagctaat caaaaattat caggtcgggt ggggggatgg 42420ctctgctcgg cggtagacat ctttctttat atcggtaaaa ataagtaagt gattacctat 42480ggcgagcagg gatgcccgcc tttacaagga gcgaatatgt ggtacaggga aggtactatc 42540acatttacac agggaagtga cgcactttct ggcactggca cgtactggaa tgtgaccgcc 42600aacggcgttc tgccgggcat gatcgtcatc ggccctgaca acaagttgta cgaaattaag 42660cgcgtaatta gcgacacaag tctgattctc gcagagccgt acacagggga gacccagaag 42720gaagttccgt gccgcatcat cacaacctat gagggcgact taacgcagtt cagcgcacgt 42780tttaccgcgc ttatgacccg tatgtcagcc gactcgaaga cgatgcgcag ctggttgacg 42840gcagttgatg aggtaacgct tgagcgtgaa gacggtacgg aagtgaccgt gaagtcgctg 42900acgcagatcg tcgatgagca caacgcaaac cagaaatggt atacggataa tgcagacgct 42960attaatgcgg caggcgagaa ggctcgtgag gccgctgagc gcgcattagc tgcggcgcaa 43020agctcttcag aagcaagagc aaaagcagat gaagccgctc aaagttcagc ttcagcatct 43080gagtataaaa ctgcggcaga gctaagtgcc gctgcatcaa aagcatcgga gcacggcgcg 43140gcagaaagcg cagcttcatc gaaggcaagt gcctctgcgg ctaaaacatc tgaagataat 43200tctgccgcgt cagagaccaa tgcggctgaa agcaaggctg ctgcggcatt aagtgcgtct 43260tcttcggcaa atagcgcctc agaagcattg caatacgcgg agtcagccaa gacctctaaa 43320gaggctgctg ctgcttcaga agcagcggca gccaatagtg aaaatgaagc cagaacctca 43380aaagataccg ctgtagcggc tgcggcagag gcatcagcta atgccacatc agctgatgcc 43440tccagacatg atgtcgatac caataaagcc gaagtatcga gaatgaaaga tgaagttttc 43500gctgctaggg actcaacgat tcagtatagc gaagaggcta aaacagcggc tgatacagcg 43560gcaagagaag cagccacgaa aacatctgat cagctcctgt cggcggttaa atcagaggcg 43620gaaaaggcaa acagtgctag cgcaagtgct caaggttttg ccgatgacgc caagcgattt 43680agagacgaag ctcaggaaat agctgaaggc agcaaggtaa acgatgcaac aacctcacag 43740cagggggttg ttcagttgag tagtgcaact gatagcgaga gtgaaactct tgcctctaca 43800ccaaaagctg tgaaaacagt catggatgcc gttgctctaa aggctcctat agatagcccc 43860gcgctatctg gcgcaccaac agctccaact ccggcaatta ctgctgctgg acgtgagatc 43920gcgacagccg cgtttgtggc ttcaaaagta gcacaacttg ttggctcagc gcctgaagca 43980ctggatacgt taaatgagct ggctgctgcg ttaggcaatg atccaaactt tgctacgact 44040attacgaaca tgttggctag aaagcagcct ttggatggaa cactaactgc gctttctggt 44100cgttcacctc aaggggtaat tgattatctt ggcttgttga atacggttaa cctggcggct 44160ggctcaattc agaaatccca gaatggggca gatattcctg acaaaagatt atttgtgaaa 44220aatataggtg cagttagctc cgccagaatt tcgtttgtta aggaatccgg gtggtataag 44280ttagcgacag taacaatgcc tcaaggagct tcaaccgctt taattactct tattggaggt 44340gctggataca acgcggggct ttatgaccag gcagcgataa gcgaaatagt gttgcgatca 44400gggaactgga atcctgttgg catcacagca acattatggc aacgctcacc agcaggtgct 44460caaggggtgg cgtggataaa tacatcagga gatgtttacg atatttatgt aaacgttgga 44520cagtactcta ttgatgttat tgctctgagc gattgtacaa ataatgcaag catagtgttg 44580ttcggcacac cagagtatgt ggcgaccaaa cctgcaagtt ccacgaacgg cgcaaattat 44640atattgtaca gtagtgttct accaccgcct gagtcatatc cagtaggtgc ccctattccg 44700tggccgaacg atgtggcccc gtctggtttc gccatcatgc aagggcagac gtttgacaag 44760agtgtgtatc cgaagctggc ggccgcatac ccatcaggtg tgttacctga catgcgtgga 44820tggatgatta agggtaaacc aacttctcgt gcagtgttgt cactggagca agatggaatt 44880aagtcacatg cgcacaatgc agccgcttcc agtacagatc ttggtacaaa accaaccaca 44940acatttgatt acgggacaaa aacgtccagt ggcttcgatt atggaacgaa atcgtctaac 45000agcactggtg ctcatgcaca ctcgctgtct ggctctacat cgagttcagg tgcccatgcg 45060catacggtaa ctgctcatac tcagtatcca agatctacag attcgaggaa ccagaatgct 45120gtcggtaagc aatacaacac acagcagact acagccaatg ctttcaatgt ctggacaagt 45180agtgcaggtg atcatgccca ctcaatctcc ggtactgctg tcagtgccgg tgctcatgcc 45240cataccgttg gtattggcgc acatgctcac tcattgagta ttggatcaca ctcgcattca 45300gtggcaattg gggcgcactc acacactatc actattgccg cttgtggtaa tgcggagaac 45360accgtgaaaa acattgcata taactacata gtgagactcg catgattata ttagtttttt 45420cggcacctgt tgcagaaatg gctgatgctt gtacatgtga atttggatat agcgaaaatg 45480tcgagataat ttacaggtcg ctcgaagcga gcgctgagtt tgactgcatt gtcagtgcta 45540cgaacagttt ggtcaggtgg atgggagcgc gaatgctgaa tatatcgggc aaggttttta 45600ccaatagttt gtgaggcttt tggttggcgt gcatcacgtc cgagacatca ggggacaatt 45660ctaatgcgca ttcttttaaa tctgtatcgt taaaggagtt gacgaaatga ctttcaaaat 45720gactgataaa gcgagaactc tcaaagtgta caatctgctg gaggggacaa atgaatatat 45780cggagttgga gatgcctata tcccaccgtt tactgggtta ccggccaact gcactgagat 45840tgaaccaccg acgactacgg aagggtttgc cgcagttttt gacttcacaa agcaagagtg 45900gagccttgaa gaagatcatc gtggcaaaac gctttacagc acagaaaccg gtgaaccggt 45960gttcatcgct gaacttggcc cgttgcccga aaatgtaacc tacatctctc caaacgggga 46020ataccagaaa tgggatggtt ctgcttgggt taaagacgaa gaagcagaga agactgccct 46080tgtcggtgaa gccgagcaga ataaatcggt gctgatgaag aacgtcagcc aacaaatttc 46140cttgcttcaa gatgcgattg atctggatat ggcaactgat gaagaaaagg aaacactggt 46200tgcgttgaaa aagtaccgtg tcttgcttaa ccgagttgat acttcgttgg ctccagatat 46260tgactggccg atattgggaa acgaggaaga agattcggca aatttgatta aataaagtag 46320gtaggtagtt atttatataa tgtgatataa atatgccatc ccgatttgac tattcatcag 46380ggtgtcaacg acggatgaaa agtgatccac ttatatctcc accaacggcc caatattgat 46440ccaccgtttt actcaggatt agcttctgct ataaccccgg cctttcgttt ctgtctgagt 46500cgatagcttt ctcctttgat ttgaacgaca tgtgagtggt gtaagatacg gtccagcatc 46560gctgaggtca gtgctgcatc accggcgaac gtttgatccc actgcccgaa cggcagattg 46620gatgtcagga tcattgcgct cttttcgtaa cgtttagcga tgacctggaa gaacagcttt 46680gcttcttcct gactgaacgg cagatagcct atttcatcaa tgatgagcag gcggggggcc 46740attactccac gctgaagcgt cgttttataa cggccctgac gttgtgccgt agataactga 46800agtaacagat ctgctgctgt tgtgaagcga actttgatac ctgcacggac tgcttcatag 46860cccatcgcta ttgccagatg ggttttcccc acacctgatg gccccagtaa tacgatattt 46920tcattacgtt ctatgaagct gagtgagcgt aacgactgga gttgcttctg cggtgctccg 46980gtggcgaatg tgaagtcata ctcttcgaac gttttcaccg ccgggaaggc tgccattcgg 47040gtatacatcg cctgtttacg ttgatgacgt gccagttttt cttcatgaag cagatgctcc 47100aggaagtcca tataactcca ttcctggtct actgcctgtt gtgacagcgc aggcgctgcg 47160cttataaggc tttccagttg caactgcccg gcgagcgcca tcagtcgttg atgttgcagt 47220tccatcatca cgccactcct ctgcagaatg agtcgtagat ggagagtgga tgatgcaggg 47280ggtgtttgtc gaagttcacc agattttcat caagatgcac gtcatactct tttttctccg 47340gaggcagtgc cagcatggac tgctgctctt cgagccagcg atcgcaggga cgggcctgga 47400ttgtttcatg ctttcgttgg ttagcgacat cgtgcagcca gcgcagaccg tggcggttgg 47460ctgtttcaac atcgacagtg atccccatcg ggcgcaggcg agtcattagt gggatgtaaa 47520aactgttacg ggtgtactgc accatccgtt ccaccttacc tttagtctgt gccctgaagg 47580ggcgacacag tcggggagag aagcccatct ccttgccgaa ctgccacagc gaaggatgga 47640accggtgctg accggtctga tatgcgtcac gttgcagaac cacagttttc atattgtcat 47700acaacacttc gcgcggcaca ccaccaaaga agcggaacgc attacgatgg caggtctcca 47760gcgtgtcata acgcatattg tcagtgaatt cgatgtacag cattcggctg tatccgagaa 47820cagcaacgaa cacgtgaagc ggtgagcgac cattacgcat agtgccccag tcaacctgca 47880tctgtcgtcc gggttcagtt tcgaaccgaa cggcaggctc ctgctcctga ggaaccgaga 47940gagaacgaat gaatgccctg agaatggtca ttccgccacg atatccctgg tctctgatct 48000cgcgagcgat taccgttgcc gggattttgt aaggatgagc atcggcgatg cgttgacgaa 48060tataatcccg gtattcatcc aggagtgaag caacagcagg tcgcggcgta tattttggcg 48120gctcagattt tgcctgcaaa taacgtttaa cggtattgcg ggagatcccc agttctctgg 48180caatcgcccg gctactcatt ccctgcttgt gcaggatttt aatttccata actgtctcaa 48240aagtgaccat aaactctcct gaatcaggag agcagactac cccctggatc tgatttcagg 48300cgttgggtgt ggatcactat tgcaccgttc gttacagcaa cgagtgattc gacagcagta 48360acggccgcgt tcccggtcag ttcgcgcgcc gccagcttct ccagcagctc tactaccttc 48420tggtcgctgg gtacggacgt atcgagtggc tcggccactt tgtacttctt cacaccgaat 48480cgaatgaatg ggttgagcat tagcgagacc atgctctgct caaattcatc aaggttggcc 48540agcgcctctt tcttggcgtt ggttcccatc gtttttatgg catccagctt gtgctttagg 48600gcgatcagtt tttccattag tgtttaacct ccatcggtcg ctcgggagtt ttcatgtgtt 48660ttctttggtt gcttcttcaa tgagtgccgc gtacacgtca gtgacgggcg ccagtgaatc 48720ggtggacgtg gttttgggtt tggctggttc tgttttcttc gtgcgtttaa ccagactgtt 48780aatcgtcatg gtgttgcgct tccgggtgag cattctggca tggtcgtttt gctcttccac 48840ttctttgata agcgcagtca tatcgatgaa gtagagctgt tcgcctttgc ggatctcttc 48900gaccatcatc ttcagcgcct ggcatttgcc agcagcaatg gccgcagcgc aggacaggaa 48960cgatgtagct gggagacgct tctctttgta ggcgaggatg gtgtgctggc agactgtata 49020gctgcaatgg gcctcatggc cgttgatctt cacttccgga cagcacagcg aataaccgtt 49080gtttccggag atagacggga ttttcgacaa atctgttctt gtggacatgc ttctaaccgt 49140agtcgtgtac ttacttattg agcgcagttt aaaaaagccc caccaggggg ctaaatggtt 49200tatcgaggct taccaggtcg cccagccagt cattttgtcc tgggcggctt cgaaccggta 49260tggctccagt aaatcgttgg catggtggac ggcgtaggat tttgcctcct gtttaatcat 49320cggcagctcg ttggccaggc gtgccacttg ccctgcaaaa ccggctagca caccgtcaca 49380tgcctgaccc gcatcgacaa tgatacgcac caggtctaag tcgctgcggc acatatcgca 49440gatgatgccg tattccacct cacgaatgcg ctcaacggct tttttggtgt cgccactgac 49500caccaattcc agcaaaccag gtggggttgt cagatctgta acgcgctcgg taacttcagg 49560cagttcgaca atgctcagga acgccgcgat tgacgggtca tcctctacac cagccctgcc 49620tttgatcgcg cgaagagtcg cgtcgacaat atcctcaaac cgttcacctt catcgcacac 49680cgcctgattg gtgtagacaa cgcggccgtc gtaccatgct ccagcacgta cttctaccgt 49740tgcatccttc atctcgcgag tgaacgtcac aagtgccgcg cgtttctgtt taacaccagg 49800cagctccgga gactctccaa aacgaaccca gacccgcatg tatttcgacc cttccgaaag 49860aggtgcggtg ctcacagacg tggcgatgtg tttcagcgca gtctggatcg cctcaccgat 49920aatcttctgg cgctcttctg tatcaatttc tacgcctgat ttgtcgatga tttcggtaac 49980ggacttctga atatctgctt tcataaaggt tcctcaattc cttcgtcgcg catattctta 50040cagaaaatta agtaagtatc tacttatcga tttaggttca ggaaaatcca tagtccattg 50100cattacaagt tcaaatattg accaaccctg attcgacaag tgctacctta tcggtttata 50160tgctatgggg cattgttacg cgtaaatgag tagcctgagc catgtctttt tctataaact 50220ccatgataga aaccatctta ccttgctgga ttatgtatgc ctgagaaaat ctttaaagcg 50280ccttgcccta catgtagagg gacatgcaat acgttagttc atggtgaaat acaaaaagag 50340tggagtaatg cggtagacag ttttaattta tcatatgggc aagatagtca taagcttctg 50400gaatgttgcg ggtgtggcac tgttttttac tataaggact catggggtag tgagcatggt 50460gataatgata tttacggaaa attcacccct acacatttta ttgaaacagt ccctgcgcca 50520gagcaaccaa aactaaagcc agactggttg tttgaaattt acaaaaaaga tcaaatccta 50580ttttttattc ttgatgaagt atatacagca tatgaacacg gatcgttcat actcgcctct 50640acagggctac gcacggcatt tgatcactca tgcgctcata ttggcatacc taatgcctac 50700acaatggaac aaaaagtcaa agatgttttt gtgaaaggtt atgtgagcga aaccgaacga 50760gatcaactca gaattgttat agaagctggt aacgctgccg cacatagggg atggagacct 50820gacaaatctg cttttgagtc actattacat gttgctgaaa aattcattca gcaagttata 50880ctaagagacc ttgagataga aaaaatcggc gaaaagatac caaagaagca aaagaaaaaa 50940ggagactgta aacaatattg tgtaattgcc tgtttttgat atcttcactc caacaacgga 51000gacaggcaaa ttatggacga aaagaaactt aaagcacttg cggctgaact ggctaaaggt 51060cttaaaaccg aagccgacct taatgcattt tctcgtatgc taacaaagct taccgtcgaa 51120acagcgttaa atgcagagct taccgaacac ctcgggcacg agaaaaatac ccctaaatca 51180ggctcgaata cccgcaacgg ctattcgtcc aaaacactgc tatgcgacga cggcgaaatc 51240gagctgaata cgccacgcga ccgcgaaaac acctttgaac cgcagctgat aaagaaaaat 51300cagacgcgta tcacacagat ggacagccag attttgtccc tgtacgccaa aggcatgacc 51360acccgcgaaa tcgtcgccac cttcaaagag atgtatgacg ccgatgtgtc tcccacgctg 51420atatctaaag tcaccgatgc cgtaaaagag caggttgctg aatggcaaaa ccgccaactg 51480gatgctctgt atcccattgt ttatatggac tgcattgtcg taaaagtccg ccagaacggt 51540agcgtgataa acaaagcagt gttcctagcg ctgggcatca acactgaagg tcagaaagag 51600ctgctgggca tgtggctggc agaaaatgaa ggtgcgaagt tctggctaag tgtgctgaca 51660gagctgaaaa atcgcggtct tcaggacatt ctgattgcct gcgtggatgg cctgaagggg 51720ttcccggatg cgataaacag tgtttatccg cagactcaca tccagctgtg catcatccat 51780atggtacgca acagcctgaa atatgtgtca tggaaggact ataaagccgt caccagcggt 51840ttgaaaatgg tgtatcaggc tccgacagaa gaggcggcgc tgatggcgct ggataagttt 51900gcggaggcct gggacgacaa atacccgcaa attagcaaaa gttggcgtac gcactgggaa 51960aatctcaata cattcttcgg ctatccgccc gatatccgca aggctatcta caccacgaat 52020gccatcgaat cggtgaacag cgtgatccgt gcagccatta aaaagcgcaa agtgttcccg 52080acagacgact cagtgcggaa ggttgtttat ttggcgatca aggatgcatc aaaaaaatgg 52140agtatgccga tccagaactg gcggttagcg atgagccgtt ttattatcga gttcggtgac 52200cgcctgagcg atcaccttta atacggtggc agttacacag aattatggac aggctctgtt 52260ttttgccaca cgtaaacgtg aaattgaaaa ctatctttgc ccagatctaa taagagatga 52320gacaggggtt caggtttttt tcacagacac atgcgacgca aaaaaaccat aggaagggca 52380accagcacga aacctaacga cgtactggac aggttctggc ccttaatgac tgccgaaaaa 52440atcataaaat gctctactta tagagatgat aataatgaca aaattgagct tatagacatt 52500cttgaaggca ttgtttcatt agtcgattga tcgtgaccta cgttgcatag cggtgtgtca 52560gttaagcctc tgtgtaacgt tattgtgctc aaaaaatgag cttaacgaat ggaagggact 52620tcccctgatt atgaacctga ttaactcttg tgcaatcatg ggataaatat cactccggag 52680ggattcgtta tgaccatcac tactgtcggt atcgatcttg ctaaaaacgt gttcgctgtt 52740cactgcgttg atcagaatgg taaaacggtt ctggttaagc ccaaagtatc gcgtgctgca 52800cttcctgagc tgattgcagg tttacctccc tgtgttatcg ggatggaggc atgctccggg 52860gcgcactact gggcgaggct gtttcgagag tatggtcatg aaccgcgcct gatggctgca 52920aagtttgtat cgccttacca catggccggt aaatcaggaa agaatgatgc tgccgatgct 52980caggctatct gtgaggctgt ccgtcgtccg catatgcggt ttgtgccagt gaaggacgaa 53040agccagcagg ctatgcagtg tttacatcgt acccgacagg gttttatcga agagaaaaca 53100gcaacgtata atcgcctgag aggattgatc tctgaatttg gcgtcatcgc cccgcagagt 53160actgatgcct tacgccgcat ggtttctgag cagaagaatt ctttaccgtt ccaggttcag 53220caatgtattg atgatttgct ggagcacgtt gatcgcattg aagccaacat tgctgactat 53280gaccgaattt tgtcccgcat ggccaaaaca gatcaccgca gtcagcgact gatggagctg 53340aagggagttg gccccacaac ggcctgtgcg ctggtcgcca gtatcggtaa tgcacatgat 53400tttaagaatg ggcgtcaact ggccgcctgg ctggggctca cgccttcaca gtacagcagc 53460ggcggaaaat caaagcttgg caggataacg aaagctggcg attcgtatct gcgaacactg 53520ctggttcagg gggcccgttc agttctgatt ggcgctgata aaaggactga ttctttcagt 53580cgttgggttt gtacgctggt tgaacgcaga ggatactggc gtgctgttgt tgccatcgcc 53640gccaaaaacg caaggctgtg ctgggcatca ttgcattacg gtgatgattt ccggctgtac 53700tcagccagct aaagcactaa gtagtataac catctgacct gcaactcgtt gatgataaag 53760ggttagaccc cgtgaggcct atctggctat tgtacaggat atacgtatcc gcttaacgaa 53820caagaacctc acgcgcgtct ttcatcaggg cccgaatcga tgacgattca tcatggccgt 53880ttatagtacc gcagtctctc ccctttattt ttactgaaga gcagacagaa accttgaata 53940ccgacgttga catgccgggg aagcccttat agtacaataa ttttcgatat ccaaactgac 54000cccaacttca agtttgcctt tctgttcacc gagttgaatt cctttctcga tgcccttctc 54060gatacctttc tgttcaagct gttgtgcgat ggtcatgagt gcgtctccat gctgcggcac 54120acgctgtgcc agttcgcgaa caaaggcttc ggagtcagca gactcgccgg cctgtaataa 54180atagtgtatc agcgccatta cctgcggtga agacagataa tctgccatca gcaacgtagc 54240cagtctgtcc gtcagggtgg ctatatcacg ttaagaaggt ttgctctgcc gccagcggca 54300ggcctatagg gtcaccacca aagcacgcca gagtgctgaa gtatcgccta acctattgaa 54360tcagaatttt aatccactgg gaattaacca ggtgtgggtg agcgatatca cctatcgtgt 54420gcccggagtt cagggcgagc atggacgctc aaatgaacca cgagtctgtc tggaatattg 54480aaccggtaac tcacgatgag aaacccaaca atcctaccgg gtgtgacggt ggagaacctg 54540agcggcagtg acctgcggca tgcccgcagg gtgatgtaac ccgctgacaa cggggattga 54600ggcgagatca ctaagccgag atgatcctca aggttaagtg ctgaaaggct gaagaacatg 54660aacccgttaa tccgcctctg tgggttgaaa atgtcaccac ggcctatgtg atctgacagg 54720ccgtgcagaa ggcaccgaca ttgatagata tgcagtgttg gtcgaaagtg ttttgacatg 54780taagcagaac accgggacag caacgtctat cacgctcgcg ttgctgactt ctgccaactt 54840gcggcaagca aggacaaaga gtgcgacggg cagcctcctc agtatgtctg agtccaggca 54900ggtgaaccgg ggaaggtcag cgacggatgt taagggggca tggctccgat gacgcgctgg 54960ctggcggagc ttccatagta gtccgcgatg gggaaagccc attacatggc gaagggaagc 55020agtttaaatg tgtttgcgac gtgaattaac tgacctaatg aggtgaagac ctttgataat 55080cagcgaaatg caacgcaagc ttgccacatg ggcagccacc gacccgcccc gacgggttga 55140acggctgctg cgtctgataa cacaaccaga atggctggct gaagcggcgc ggatcacact 55200tgcatcaaag ggggcgcata cccccggcgt tgatggtgtg aacaaaacaa cgctacaggc 55260cagactggct gctgagctac aaatactcag ggatgaactt ctctctggtt gttaccaacc 55320tctgccagcc agacgggttt atatccccaa aagcaacggc aaacagcgac cgctgggtat 55380ccccacgctg cgggatcgta ttgttcagcg ggccatgctg atggcgatgg agcctatatg 55440ggagagtgat tttcatactc tgtcatatgg cttccggcct gaacgcagcg tccatcatgc 55500gatcagctca ccgattgtgg ggaaacacga gggcgctggg taattgaagg cgacctgtcg 55560tttaacttga ccagaacagt ccagtatggt cgttattaga aagtccactt cgctaagaag 55620acttggggcg catggcacat gcgtaaaata tctcggacgc tacctaaaac gaccaccgat 55680gtcggcgtca aaactgcgac gaaacagaga tgataaccat catgattatt agcatgatgg 55740tttatgtgat ttatgactga ttattactta ctagtgtatt ctagtcatca atttgggctt 55800aattttggaa atgcatgtgg gctagaatat ttccacaatg gttcccagta agatttcatt 55860aattcattaa ctgcatcttt tccttcgact aaatagtcaa actctgacag ataaccggga 55920taaagattat ctgatccaac aacgtacaac tcatcgtcaa taatcattaa tttggcgtga 55980ttaccaggtg caacagggac tttaggatag aaaagtccac tacctttaat tgctgacatt 56040agtgcactac caataatacc ttgatgcggg ggtttttccg aaagtggttt ttgcttaagt 56100gttgcagtat aagcgctttg ttctaaatca ggccacttgt aggtttcgcc ttcaattgta 56160ttttcatcag gtactttatc tgtaaagaag aatggtgcaa tcaatattct ttttaaggca 56220tcagcacggc taccatcagg atcgtctaat acttcatcgg tatcaatatc atgggttagg 56280taatacttaa ataattcata ggtccgttct gctccagaac caaatgagta ctgatcacca 56340gcagctccag ctgctgcatc tagagcagag actacaacat gaatatgaag atctttattt 56400tctaacaaag cctcaataat ccaattacac gtaaagtggt ctttccattt ttttttccaa 56460gcactcacga gatcttgctg tgaaattcta attatgcgct tagcattttt tatcagttgc 56520tctttcatta tttcagaccc tctttggtag tcatgctcca tattaggtcc tgtccaatat 56580ttacctactg ataaaactct gtctgctttt ttatactctt ccatgttttc gtattcacta 56640atacgagtcg ctaccttctg attaaagttt tcatgcaagt tgagtaggtc ctcttgccgt 56700tgcttcatat aattcatagc aactgagctt ttaagcggat cttcaggctt atcatagaac 56760tttgttccga ccgcccacat catgctttca taatcaaaat attctttttt tagtaaatct 56820gaattacatg accatagttc gttaagatat agctgggagc cataagcaga agaaccatga 56880gtgataattg atacatcatg aacaggtgga taatttctaa atagatccat gttcatgtta 56940tgtccaccaa caagagcttc agtaccatct gaggccatta tttttgtgtg attccatgtc 57000attcttgtat cgttgattgg tgggaaatca ggatacacgt ttctcataaa tgatgtagcc 57060aacccttctt ctattctaaa gaaacggcct agccaaattt caggcatttt ttcccaatat 57120tgtcctcgct ccttaattag ctgaattaac tctgatttaa aagcaacaaa gtcaggggag 57180ccatttgttg cagcagataa cccattcata aatactgttg gagattggcc gaaaagaaac 57240ctatactgag ttggctgagt tcgccccatt tttttcgata aggattcatc aatggcctta 57300aaaatcactt ttcgccactc tgcatcgggg ctattaagtg atgatatatc acagcgataa 57360cgcgaattcc gcagtacttc agtcattgcc cctgcaaact cctcttgccg taagtaagat 57420tgctgcatga tttctttacc aaatggggct ccccaagcat ggggggtatc cagaagtcgt 57480atataattaa tgttacttag atgatgaaag tagtttccaa tattattaat gacattatct 57540atttgaagca tattttatcc tttgtttatc gatgtgttaa taaatatcgc cgttgcagca 57600attaatagcg gggagatagc ggggtacttt acgcccatag tcggttaaac tcaacctgat 57660attttcccgc tatttcaggc gcaccacgta tgacgagaac attttctgca ttgtgagtat 57720cgccattggt ggtgtaattc attgaccctg tttgtatcgt gtcaccatca gctatcatca 57780ccttgttatg atgaattgaa tagttgttat ttaaccttac cggaacatgt tgttgtgcca 57840gatagtgaat ggctgaatag ttcagacggt tagctttggc atcagcaaca actcggacgt 57900taacccctcg cttttgagcc gagacaatgg ctgtcgagat ctgcttactt gtaaaagtat 57960aggcttcaac atcgagcgag gattgagcat tattgaccac gcttaataca ttctctaatg 58020cagtgtgaga aggagagaat ccaacattaa aggacggggt tgcgagcgcc gaagcgctga 58080ccagtacaaa aatgatgact gagagtttaa atctcatgag ttatgtccaa agtttgtagt 58140tcatccatta tgtaaaaccg aaaaaaaacg cctatgtttg gcttgctgta tcgagctttg 58200gcttttatgc ttcgctcggt ttccattgtg gcgatccgtc ggccgttcgg taaggtaatc 58260agcccatcag gacggtgctt gaccttaaac tgatttaaaa agtattacgg tcacttttaa 58320tctaattttt agccccttct tgggcgaata tcaagtgaaa acgctgttgc gataaggtct 58380gctaaaaaac aggcttaccg tacatttttt gaatattaaa cataatgtag gctgcatcac 58440catataagtc cacatacata gtaatggtat gcgatgccta cattttgtga ctaactttgc 58500ataatcatat aagttttata taaaagggtt tgaggttcag tagaacggtt ttacatctta 58560atattttgta tgataaatca tagtgttata gataacaaaa aacaggtgca taggggcgtt 58620tttttagggg tctgaagcta agtcaaacga aaactcacgt taggcgagaa aggtcgtctg 58680aaaaatcgat taatggacag cgatgtccgt taaatgctat ttaccgatta aaaagacacc 58740gttttaggcg catttttcac tgtctataat gttttgattt atcggacaaa aagcccttaa 58800cgtgtgaaac cgttccgttg agcgtcaaac ccctattaac gtggaggacg tatcaatgtc 58860gcgtcgtggt ttacgtttat cacagctgcc accggtacgt tcggaaaagc caaaaccggc 58920caaaacgtcc gcgcagctca tggcggaaga tacgctgcgt gagttaattg ccgcccgctt 58980tgccgtggga aaaccggtta tccacattca tgcaaactgg aatgatgcgc tactactgcg 59040ggtattgaaa gaagcgatac accaagctaa aggcccgccg tttgtggtgg taccaccgca 59100tctaaatgaa catgagaaag agaacgacta gcgagcgcta gtcgtttaaa tgattagtgg 59160cggatttgcg ccaaatcgtc ttcagaaagg ccggttgctt cttggaccga ctcaatcggc 59220atgcccattt ttaacaaact gcgtgccact tcgagtttgc ctttctctat gccacgttgt 59280tcaccgagtt gaattccttc agtacggcct tccatacgac ctttttctat gcccttctgt 59340tcaagctgtt gtgcgatggt cataagtgcg tctccgtgtt gcggcacacg ctgtgccagt 59400tcgcgaacaa aggcttccga gtcggctgac tcgcctgcct gtaataaata gtgtatcagc 59460gccatcacct gcggtgaaga gagataatct gccatcagca acgtcgccag tctgtccgtc 59520agggtagcaa tatcacgttg atgaatatgc ttttgcagca gcgtcagtgc cgccatgctg 59580cggtgctcca taatatcatc gtctggaata accgtcacat caaccagtgg aaatgcaccg 59640ctatagagtt tatgtgccaa ctcagggtcg tcaaactcat ctaaccaccg cgtggagtac 59700ggataaggac tgcgtttacc cacgtaaaag agcaccggta tcaccagcgg cagcttagca 59760tggcccgctt caaggtggcg ttgcatggca gcgatcgcgt aacgaattaa gcgaaaagcc 59820atatgcttat cgggtgagct ttgatgctcg atcaacacat ggacgtagcc ctcgccttcc 59880acggtatcga ggctgtaaag cacatcgctg aagtactgac gcaaatcatc ttcaacaaag 59940gagcctgact ccagctttag cgtgctgagg tcgcagatag cgcgtagctc agcgggtaga 60000tgcaactcca taaagtcacg tgcaatctcg ggctgggtga gaaactgcct aaatgtggca 60060tcatggggtg tgggggtact gtttttcttc ttcatcagct cagactctga aaaatgatga 60120gtgaatgcta tcacaatcaa agcaatacaa gatgttaagt ttacccctca gcttgctggc 60180agacgcggat caccgtcatt ttggataccc ctgcgagacg ggctgtttca cgcaatgact 60240taccatgcaa taggcgtagc gttcggatga gctcgtgttt ttgtgcatca gccaccctgc 60300cccgatattt accttccgct ttcgctttgc tgatcccttc cgcctgacgg cgacgtcgat 60360cctcataatc ttttctggat atagccgcga gcatatccag catcatgcca ttaacagctt 60420tgagcatgct gcgagtgaat tcatcgctaa cggcgttatt gagcgccaaa tgactggttg 60480gtaaatcaag actcacgata gacagctgct tatccgttat ctgctttctt agcgcttccc 60540atcctacgtc gtccaatcga gaaagcctat ccacttgctc aatgagaata atatccccag 60600gctcagcctc ttccaaaagt tgcacgagtt ttggacgtgt catcgttgcg ccagagatat 60660tgtcgacata ccagcctgca attcggtggc catgtaggcg agcaaaattc ttcagtgcat 60720tttttgcgcg agtggcatct tgttcagagg ttgatgctcg cagataacca aaaatgagca 60780ttttttagcc ttgaggtaac gtttaagtag tcattttgtt aatggtatca cttatgtggt 60840cacattgata tggaatacca tgttttaatg cggtatctca gaagtatacc cgaatgtgac 60900aggctgacgc gcccgtaggg cttatccaac ccttcgcctc agatgcaagc tctgaacaga 60960ctcacggttt tcgtttctgg tcaaattcta attaggcgtt taaagagaag gttttgtata 61020tcctgcctgt gggatagtat tgcgtgtgct gttattacat tatcctccac tgagtacaat 61080actctgtagc cgtcaggggt gttgcactca cgatatttag cacaacctat cttgagtaac 61140tccgggcaga cctgacaccc aagtggaaaa tcactgactc gtttttcgaa gtgctcaatg 61200atgccgctga tcacttcctt tggttctgac tcgatatgac gtaagtggct cgcaatatca 61260tcaatgcagg ttttgacagt tctggtgtac tgaataacaa tcgccattac agacccgcca 61320gtagttgttc tttactgaaa atattgccgt ctgccttatc ttgctctgaa agcgtcagca 61380acttcagtag tgcaatcgcg ttctgtcgct cctgttgcgc gtcgtatgac tcaataacat 61440atgccggaac cccattttga gtaacaagaa ttggttcttc cagatcgagt gacgctgcgt 61500tttttttcac atagctaatg gtttcaattt tcataacaac ctcacataac aaagaggttt 61560aaatatagtc tatatttggt cttgttgcaa caatttctgg acagaatgac ctgaatgtcc 61620tagctgtgta gtgattactt gttaccacca taccctccac cccctcgcct gctcagaaac 61680gatgtctaac tgcgcggttt gatgtgaatg atggttggat acgtacaata atttaatgac 61740aatacaaaat atgttgtaca ttttttgtgt gtatgtacaa tgtgattgaa ctttataggc 61800aatatatggg cagggaatct atctcagggg aaactatgaa acgagattac ggtagtgtcg 61860gtaccatagc gctcagagca agtgctctac ttcaggcaat gagtcgggat attgaggaac 61920aaagaaaaga attcaatctg acagaatatc atcaaacata tactcgtaat gcggtcgcaa 61980aattgcctaa gctgagccga cgcatcgtag agctggccgt taaagaaatg gaagaaagcg 62040gctatgaatt taataaaaag caggttggca acgtcgagca atatgcacta acaattcaga 62100acgttattga tatatatgct caccgacaga tacctaagta ccgtgatatt cataaagcac 62160cttacgtaat ttttgtcgtc aacctaaaag gtggggtatc aaaaacagtc agtacagtaa 62220cgctggcaca tgcattacgt gtacatcagg atttgcttcg ccatgatctt cgtattttgg 62280ttatagacct cgacccgcag gcatccagca ccatgtttct ggatcacacc cacagcatcg 62340gcacagtcct tgaaaccgcc gctcaggcaa tgctgaatga tctggatgct gaaacgttgc 62400gagaagcggt tatacggccg actattattc caggtgttga tgttatacct gcttctattg 62460atgatggttt cgtggcgagc cagtgggagt ctctggtagc agaacattta cctggtctta 62520aaccttctga agttctcaga aaaacaatca ttgaccgtat cgctggtgat tatgattttg 62580tctttattga tactggccct catcttgatc cattccttct gaatggctta gctgcgagtg 62640atttgctttt gacccctacc cctccggcac aggttgattt ccattcaacc cttaagtatc 62700tgactcgttt gccagaaatg cttgagcgtc ttgaagaaga aggtgtagag ccacgcctga 62760gtgcaagtat tggatttatg tcaaaaatga ccagtaagcg cgatcacgaa acgtcccata 62820gtctggctcg tgaggtgtat gcgagtaaca tcctagactc atcgctcccg aggttggacg 62880gattcgaacg atgtggtgag tcttttgaca ccatcattag tgctaaccct gtttcgtacc 62940cgggaagtgc agaggcattg aaaaaggcac gtactgaagc tgaacggttt actaaggccg 63000tatttgaccg aattgaatat atcaggggag cgtcaaaatg aaacaggtta tagctcgtgg 63060tcgggttctg ggaaatagta attcagagtt cgctagaatg ctcgaaggcg acggtgatgt 63120taaaacattt acccttaaat caggcgtgca ggctaggttt gtcaaaacag ttgtgttaag 63180cggagaagtt gaatcaaaaa cgttcgttga tgcttcggtt aatggacgtg atcagacaat 63240gcttacgcgc gagtccgtca gtgatatttc ccggacgata aagctgcagc agttctttcc 63300ggctattggt cgggaggtta atggactaat tgagatatta gatggaaccc gacgtcgtgc 63360tgcctgcatc tttaataacg ttaaattcga aattctggta acaaaagatg atatctcact 63420cgccgatgca cggcagttgg cgaaagatat ccagactgcc agagaacata gtcttcgcga 63480gctggggaag cgactcgaag ttacctacgg aaccagcatg acgaaagaag atattgcgtt 63540gaaagaaaat ctctctccgg cgaaagtgac acgtgcgttt caggccgcag cagtgccaga 63600cgaaatggtt gcagtgttcc cggtgataaa tgatatttcg ttgtcagatt atcagttttt 63660actgaaactg gccgaagaag caaacaacaa gcaaacatcg gtaacagagc tgatggaaaa 63720agttcagcat cggttgaaga ccatgccaga ttatccggca attgataaaa gcaaaattct 63780tgcggttatc cggtcggaga gcaaattgct gacagccctc ccaactagaa cggttcaaac 63840agagaagctg agagaatttt cagatcgtaa tcagtttgcc agaaagaaaa ctgatccaaa 63900gaagcgactt gttgtttatg agttttcccg tatttccgct gaggcacagt cggagattga 63960taaggcaata aaacgtattc tggaaagact tccagaatca ggtgagtaag ggataaggat 64020gccgaagagc atcctttttt gtatgttttt caccacgcca atttcatggt tatttgtttg 64080attataaagg aaaattgaaa attctttcac actgaaatca ccacgctttt caacctcttc 64140gtgactcata atttcgctat tggcagttca gaccagcgag ttgtataggc aggcgaaagc 64200atctcccgtt ttatatgcca ttcagacgct accccttcca gcaaaccaca cttttcccag 64260acatgaatat ccatgtatct catctgttcc aacttatgta atttactttc tccatttaca 64320ctgcgttgcc atgcactcgc tttcaggaaa tcagtataat agcccctaaa tcatcttctc 64380ggagtgaatg gacatggcta ttgcaggttg cgatttacca acttttgcca cccggctcaa 64440tgaagtgctt actctcagtg tgccagtgcg tacacctgaa aagctttttg ggcgggataa 64500gcagttggag acgatacaac tggcacttca ttcaccgggc cgacatgtgt tcatttatgg 64560cgatcgcgga gtaggtaaaa cctccctcgc ccacacagcc gcttcgctta tccagtcttc 64620ggataaccgt cctattaccg tcagttgtga ccatgactcg accctggaaa cagtcatcga 64680atcggttatt agccaaggaa tgatgcgcat gccggtagac cggtacaaaa cgtctgcaac 64740ttttggtctc aatattcctg ttttaaaagc cgaagcccgt gttgaagagc gtgaaacttc 64800tcgcgttcgt tcagtcgtta atatggccag tgctgttgaa gccctgaact atctgacgga 64860acgctattcg gataacacgg ttattgttat tgatgaattt gacctgatcc gtagcgagga 64920gcagcgtgcc cgctttggtg ttttgctcaa gcagttaagc gatggggatg tacccgttcg 64980tatcatcttc accgggattg ggcagtcggt atctgatttg attggcggac atctgtccag 65040tcagagacag attgagcagg ttgatcttga acgtctgcac tggacaggtc gtcagcgtat 65100cgttgaaagt gcatttagat attttgatat taatatccct gatgatatcg cagaccgtat 65160atgcgcgttg agtgatggat ttccctacta tgtccatctc atgtgcagca agctccttca 65220tgagtgttac atggcagatg aagtcgttag cacagttaca cgtgatctat ttctagcttc 65280gctcgatgca gctgtattgt ctgctgagga aacgctcaga tcatgttatg aggcagctac 65340ctgccgagat gagcatatgc atcacattct ttgggcgatg gctgaaggtg cagacctcaa 65400cagaatgaaa gaccacatca ttacctccta tatccaggtg atgaagtacc tcgacattga 65460acctctgacg cagaaaaact tcgacagtcg ttttgctcgg cttcgtaaag agaatcacgg 65520atctattctg tgccatgcgc ttgtcgggaa ggatggtgtc cggccaggat ggtttcggtt 65580cagggaaaac atgatcagag gctttgtcag aatgcaggca gagaagtgcg gaatagttct 65640ggattttgat cggcagtata gcgcccatac agcaagtacc agaacagctg cagttagagg 65700ggtatacaat cccctcagca cggtggaacg cagcgtagct cgcctcagac gtgatgatga 65760aaaggaagct gaagaaaacg aataacgtat ttgtaaatga atggaatacc cgcgaattat 65820atatgcaata ctcgggtatt cagcgtttgc atcatgtcga gttacccgta cagcggagtc 65880ttgctcttct cctgtctctt gatcaggcaa ttactcaatt ttcgaggcta acatcttaat 65940gctgttttag tgtcggggga tgctgctaac atcttcttca ggctgtgggg cacgaccagg 66000cgcattgaac gtgatagtga tgatctttct ggccagcccc tcctctgaaa gggcatcatc 66060cacactgaca tcacgaatca gtggactttc ctcttctata tgtcgggcaa tatcccgcag 66120ccactctgcc agcatttctc tgtccatctt tctccgctcc ggttgtgtac attcagcatc 66180aacataccac accagaaaaa gtgaagtctg cgtagtgtgg ttaaaagatg accgttttgt 66240caggcgcgct gtcatccgtg ctggtagcga cctgcgcgga ataccctcat tttatacaca 66300ggattgccca ccgaaattgt ggacagcaac gatgaaaata cgatgtcgct gcgctcctca 66360ttcatatcgc tctgctgtga acgccgtgcc gctactgtag ccgggccgct gcgaatgcaa 66420gggttcgctc tgccggtgtc ctcacccggt cgcagtttat ttcaaaggct ttacccgctg 66480tcttcccgtc gttttccgtc tcaattttcg ccgtcaaacc gtccagccag cgtgggcttt 66540ttcactgcgc tgcggcttgc ggcaccgccc ctgcattctc cgcttttcgc ccggctgtcg 66600tatcggcacg gcgtaactgg cgccgcattt gagaaaggag aactaccatg tcccgattta 66660tccagggtaa ttgcgtccat atcatgtccg gctttccgga taatgctgtc gatttcattc 66720tcaccgaccc gccatatctc gtcggttttc gtgaccgtca ggggcgtacc atcgccggcg 66780ataaaaccga tgaatggctg caacccgcct gccatgagat gtatcgcgtg ctgaaaaaag 66840acgcgctgat ggtgagcttc tacggctgga accgcgtcga tcgctttatg gccgcctgga 66900aaaatgcggg attcagcgtt gtcggccacc tggtgtttac caaaacctac acatcgaagg 66960ccgcatatgt gggctatcgc cacgaatgcg cctacatcct tgcaaaaggc cgtccgcccc 67020tgccgcaaaa cccgttgaat gacgtaattg cgtggaaata ttcaggcaac cggcaccacc 67080cgaccgaaaa acccgtaacc agcctgcaac cgctgattga gtccttcaca catcccggcg 67140cgattgtgct cgaccccttt gccggcagtg gctccacctg cgtggccgcc ctccaggctg 67200gccgtcgtta catcggtatt gaattgcttg agcagtatca ccgggccgga cagcagcgtc 67260tggccgccgt ccggcgcgcc atgcagtacc cggccgctaa cgacgagttc ccggaggccg 67320cgtaatgaac tatgcaggac acgaaaaact ccgcgcagaa gtggcggaag tagccaacag 67380catgtgtgac ctgcgggcga cgctgaacgg gatggagcac cgctatcgct ttgactctga 67440tgtgctggtc gaacgcctga cccgtcagac cctttttcgc atcaatgccc tgtttatggc 67500ggcatacaac gaaatgcttg agctggatgc ctgctttaag gactaaggag aaaaacatgt 67560acggaacctg tgaaaccctc tgccgcttgt tgcgtgagca gtatcccgca gaaaccccgc 67620tgaacctcat tgtctggtcc ccggcggata tcgaagcact ggccgacgga atggaatatg 67680ccgtttcgga acaggacaca agggcggtac tggcgcgtat ggacgccata ccggaagaac 67740aacagcttga gtcgggcgtg tctgccggtg cagtgatgga tctgattgaa caggtgaaag 67800aggcagttcc ggcggtgatg gtgccggcgg atctgcttga aaccctgcta accactgccg 67860aacaggcgtt atggcacagg gaatggaccg cccgtgacag caatcatccg gtcccggaaa 67920gcgttacccg ccgcctggct gatgcggcga aagttcgcgc attactgaaa aaatgaaatc 67980aacacgccgc ccgggcggcg tgtactgacc ccgttcagag gaacccatta tgcaggaaac 68040caccacgtta aacgcccttg tgatgcgccg tgcgcgcgat ttgattgctg attatggatg 68100gcctgaccat accgatgttg atcagcgtga tccggtcaat aaaccgggat ggataagcat 68160ttatgtccgt ctggatgcgg caaatattgt tcatctgctt cctttacttt gtagcggtga 68220catacccgca gagctgcaaa atgccatgac aaaaatagcc gggacgtcag cgcagattat 68280tttatccggc agccgctatg ccgacgcgcc gcagcttccg gaggacggaa cacagatagc 68340gttcccctgg gccggggaat ggctgacgga gccggagatt caggctgtaa cggattgcct 68400gtcccgcgcc gtgcgggatg tatcccggca ggtatgggag gatgcgcgcc ggataaaggc 68460ggcgctgacc acgcgcgggg aaacgctgtt ttatcgtcag acccgaaatt tccgtctggt 68520tgtgaaggaa aatgatatgc cctgctggct ggacgatgat gacaatttgc cggtggtgct 68580cgatgccatc ctgaacaaag gggcacgcta cagcagcgtc gagttctttg ttatcagcga 68640caaagttgat caaatcctgg catgtggcca gatgtgcgat gtgctgcgta ttccgggcga 68700gcctccgcgc cggtggatgg atttaaccct gttacatgag gtcatggcag aagcgcgtgc 68760agaaatcagt cttgtccgca atgccctgtc agcaatccgg ccagtgtagc gaacagcagg 68820gggcggcggc cccctgaatc taccacgaag gatgtcgccg gctccgcttt tacggctctt 68880cctccacgtt gttccggctt cgcggtgagt aacgtcccga gcggtctgcg gcttacgccg 68940tgcctctact ctagcccgaa cgctgcaaat gcaagggttc actgacgtcg atgctttcac 69000ccggtcgccg tatgtttaaa tgcctgtcgc ggttcttccc gtcgttatct cgctcaatat 69060tcgctcgcta accgtccaga ccctctccgc tatttaccgg cgctgcggct tcatgcatcg 69120cccctgcatt tttcgctccc tgtcgggctg tcgtgtcggc acggcgtaag ccgtaactca 69180gacaacatcg actatggagg attttttatg cgcactacga ccaccacacc ggctgtttat 69240gtgggtacgt atcacaaata caactgcggc agcatttttg gcaaatggtt tgaactgacg 69300gagtttgacg gcagggagga tttttacgag gcctgccagg cgctgcacgc cgatgaatgg 69360gacgcagaat ttatgttcca ggaccaggaa ggtatcccgt cgcagttcgt ctctgaaagc 69420gccattgact gggattttat cgccgcttac aaacgcgccg aagaagaggg cagggaagcc 69480gcttttatcg cctgggcgga gtataccggc gagtgtgact atgacgcgtt tgatgatgca 69540taccgcggcg aggcggaaag tgaggaagac tatgcgcagg agatggttga cgataacggc 69600ctgttaaatg aggtgccgga gccgctgcgc agctacttcg attttgaggc gtatgcccgg 69660gatttgttca gcagcggcta tgtgttccat gacggttatg tcttcggtaa ctgattttcc 69720ccggcaggcg gtttagccgc ctgtcagccc gcgcgcaaaa ccctggcagg ctctgcctgc 69780cggcgaagta cgccgcgcct gcggcgccgc gtatctccgc cagtgcctga tgtcccggtg 69840gtgtcagggt atgaataccg gtttgttgca gcgggtcggt gttatttctg ttccggtgtt 69900aatacctgcg ctgccgtagg caatagtggc cataccggtg gcgtactggc tgatatcgct 69960gtcctgtcgg cttttcttcc cgggcgatat gactgtgggc gacttcccta gcgggaaccg 70020gcgctgtcgt aaacggagcc ggtaaaccgt ctccgccctg tcgggcttcc atcgcactgt 70080cgcaggttca acatcacgct tgcggcatcc gtaaacggat gcgccgctct caccagcccg 70140gcggtaatgc tgccgggctt cttcggggca acgccgtttc gcgcccgcgc gttccgtcgt 70200tgcagcgggt tcctgctcat tttctcctgg tcatttcacc cttttgagtc ctttcgccgg 70260ggctgagaca agcacagccg ctgtcagctg tccagggtaa atggcacgcg gtaaaccgcg 70320ccctggacat ccgccatcgc ccgcgctggt ttacgcccct gcggcgaacg gctctcaaac 70380gggtgatggt ttttaaaagc aaaacaggtt cagaaggagg agcaacatga gcgaggcgct 70440ggcggtttta cccgacgaca cctttacccg cgaacaggct gaggtcgttg cggcgcagta 70500caccaacgtg gcaattgagg acgatcaggg ggcgcatttt cgcctggttg tccgtcagaa 70560cggtgaaatg gtctggcgca cgtggaactt tgagccgggc ggcacgtact ggctcaaccg 70620ttatatcgcg gactacggta tccgcaagcc gcagtaagaa agaggtgccc tgccggagcg 70680cgaactccgc agggccagac aatcatcaat atccagtgag gtatcaacta tgtcagtaac 70740cgatgttaaa gcaaaagccc ccaaaaaagc gagcagcaaa aaaatcacga aggcgcagga 70800agaagctctg aaagccgccc ttgaggccgc tgtcatcgag tatgttccgc tgtccagtct 70860cgctaaatcc ccgctgaacg tgcgcactat cccgtattcc gtggacagcg ttcgcggtct 70920ggctgactcc atcgaggcgc tcggactgct gcagaacctg attgttcaca ccctcgcgga 70980cggacaatcc ggcgtggccg caggtggtcg ccgactgact gccctgaatc tgctggcgca 71040ggaagatcgt cttgcggctg atcataccgt catggtgaag cgagtctcag acgacattgc 71100ggccctcgcc tcggttgccg agaacgagca gcgcgccgcc atgcatcccg ccgagcaaat 71160cgcaggtttc cgtacgctgg cagagcaggg caaaactccc gcacagattg gcgacgccct 71220cggcttcggc tcccgccacg tgcagcgcat gctgaagctg gcaaacctcg ccccgtccct 71280gatggagaag ctcgcgcagg acgaactgac cgttgagcag tgtcaggcgc tctgtctcga 71340ggacgatcct gcccgtcagg tcgaggtatt cgaaaacgtg aaggccagct ggtcgaacgc 71400gcccgcacac ctgattaaac gcgctattac cgaaaccgag atgcgcaccg acaacgccaa 71460atttcgtttt atcgggcgcg atgcctacga ggcagcaggg ggttacgtcc gtgaagatct 71520gttcagccag gacgagggcg acggcacggc agacagcgtg ctggttgagc gtctggtgca 71580ggagaagctg gagcgtatcg cgcaggacat tcagcagcgg gagggctggg catggagtcg 71640cggacgcgca gcccgcatct ggtaccacgg cgaagacggt aaggagttcg tgcagcctgt 71700tgaacccgat ccggtgtaca cccctgagca gcagcagcgt cttgatgcgc tgcgggagca 71760gtacgataca tatgacagcg tttgcgacga gtcagatgcg atcgaagcgg acatcctcgc 71820cattcaggag gcggcagaag ccagcgcgtg gactgacgac atgaagtcag gcgcgggagt 71880gatggtcagc ctgtacgaag ggcaggtgta cgtgcagcgc ggtgtgcgcc tgaaagcgga 71940tatgccggaa gaaaccgtaa ccagcagcgt aacggtgcca ttcacctcac gccagcccga 72000cgccgcagag gggatcagcg ttccgctgct cactaaaatg acctccgagc gtacgctggc 72060agtacaggcg gcgctgatgc agcagcccga aaaagcggtg gcgctgatgg tctggcgcat 72120gtgtacctgc gtcttctcgg gctgtctgac cacgacgcac ccgttccgta tcagtctgac 72180cgtgtcccac ggcagcctga cggagaacgc cccgtccggt aaggatggcg cagcgtttga 72240gatgctcatg accgaacggg caaggctgaa agccctgctg ccggaagggt gggagaagga 72300cttcaccacc ttctttgccc ttgacggcgg ggtgttgatg tcgctgatgg ccttctgcac 72360ggcctgttcc gtggacgggg tacagacccg cgatatgggg cacacctccc gaagtccact 72420cgatacggtc gaggcggcaa tcggattcca cctgcgcgac tggtggcagc cgacgaagga 72480caactatttc ggtagcctga aacatccgca gattgtggcc tccctgaaag aggcggggct 72540gacgggcgcg gcgggtgacg cggagaagat gaagaagggc gatgcagcag cgcatgcgga 72600gcactttatg cagcacaccc gctgggttcc ggcatggctg aaaggaccag agccagcggc 72660tgaatccggt gctgacgacg cggtttccga taccgacagc actgacaacg acaccaccaa 72720cacggcacac gccgcctgat aacggagagc cgcccttgtg tgaacagggc ggcaattgta 72780acgaacggtg caatagtgat ccacacccaa cgcctgaaat cagatccagg gggtaatctg 72840ctctcctgat tcaggagagt ttatggtcac ttttgagaca gttatggaaa ttaaaatcct 72900gcacaagcag ggaatgagta gccgggcgat tgccagagaa ctggggatct cccgcaatac 72960cgttaaacgt tatttgcagg caaaatctga gccgccaaaa tatacgccgc gacctgctgt 73020tgcttcactc ctggatgaat accgggatta tattcgtcaa cgcatcgccg atgctcatcc 73080ttacaaaatc ccggcaacgg taatcgctcg cgagatcaga gaccagggat atcgtggcgg 73140aatgaccatt ctcagggcat tcattcgttc tctctcggtt cctcaggagc aggagcctgc 73200cgttcggttc gaaactgaac ccggacgaca gatgcaggtt gactggggca ctatgcgtaa 73260tggtcgctca ccgcttcacg tgttcgttgc tgttctcgga tacagccgaa tgctgtacat 73320cgaattcact gacaatatgc gttatgacac gctggagacc tgccatcgta atgcgttccg 73380cttctttggt ggtgtgccgc gcgaagtgtt gtatgacaat atgaaaactg tggttctgca 73440acgtgacgca tatcagaccg gtcagcaccg gttccatcct tcgctgtggc agttcggcaa 73500ggagatgggc ttctctcccc gactgtgtcg ccccttcagg gcacagacta aaggtaaggt 73560ggaacggatg gtgcagtaca cccgtaacag tttttacatc ccactaatga ctcgcctgcg 73620cccgatgggg atcactgtcg atgttgaaac agccaaccgc cacggtctgc gctggctgca 73680cgatgtcgct aaccaacgaa agcatgaaac aatccaggcc cgtccctgcg atcgctggct 73740cgaagagcag cagtccatgc tggcactgcc tccggagaaa aaagagtatg acgtgcatct 73800tgatgaaaat ctggtgaact tcgacaaaca ccccctgcat catccactct ccatctacga 73860ctcattctgc agaggagtgg cgtgatgatg gaactgcaac atcaacgact gatggcgctc 73920gccgggcagt tgcaactgga aagccttata agcgcagcgc ctgcgctgtc acaacaggca 73980gtagaccagg aatggagtta tatggacttc ctggagcatc tgcttcatga agaaaaactg 74040gcacgtcatc aacgtaaaca ggcgatgtat acccgaatgg cagccttccc ggcggtgaaa 74100acgttcgaag agtatgactt cacattcgcc accggagcac cgcagaagca actccagtcg 74160ttacgctcac tcagcttcat agaacgtaat gaaaatatcg tattactggg gccatcaggt 74220gtggggaaaa cccatctggc aatagcgatg ggctatgaag cagtccgtgc aggtatcaaa 74280gttcgcttca caacagcagc agatctgtta cttcagttat ctacggcaca acgtcagggc 74340cgttataaaa cgacgcttca gcgtggagta atggcccccc gcctgctcat cattgatgaa 74400ataggctatc tgccgttcag tcaggaagaa gcaaagctgt tcttccaggt catcgctaaa 74460cgttacgaaa agagcgcaat gatcctgaca tccaatctgc cgttcgggca gtgggatcaa 74520acgttcgccg gtgatgcagc actgacctca gcgatgctgg accgtatctt acaccactca 74580catgtcgttc aaatcaaagg agaaagctat cgactcagac agaaacgaaa ggccggggtt 74640atagcagaag ctaatcctga gtaaaacggt ggatcaatat tgggccgttg gtggagatat 74700aagtggatca cttttcatcc gtcgttgaca gttgcctcaa tgtgcgctga cgggcaggac 74760gtgtttcgtc gcttcctact gaaagatccg aaagccggtg tcggtatcag tctatgcaac 74820aaggtgttcg aaaacccaat tccgaagttt gaggtacagc tggcgtctcc gtacaaggag 74880aaaggcgaca aatacccatt taaaccaaac ccaaaggcca agtggccaat gatcggcagc 74940ctcaaactcg atggtctccg ggttatctgc gaagtcatcg tggatgagga agaggtgaac 75000ttcctgacgc gcaccggcaa tccgattacg tcactcgatc accttaaacc ggccatgctg 75060gagcgtggcc ggctctccgg tttcaagcac atcttcttcg atggtgaggg tactgcaggt 75120acgttcaacc agtccgtgtc ggcgcttcgc aagaagaacg tgaaagccat tggtgccgtt 75180taccacatct tcgatttctt cttaccagag tggcgtgctc aggcaaaaag caaagagtac 75240ctgaagaccg gtatgaagct gaaagagcgc ctggctatgc tggtgtcatt gttccgcaac 75300acttgcgggg aagattacgc gcaagatatc cacctgcatc cgttctacat catccatagc 75360catgaagact ttatcgaacg cttcatgaag cgcctggatg agaatgaaga gggggagatg 75420ggcaaagatc cggattctgt ttacgagttc aagcgtaccc gcagctggtg gaagctgaaa 75480gacgaggatt ccgaagacgg tgaaatcatc gacttcgagc caggcgaccc ggactctggc 75540tttgcgcata cgctaggcaa gatagtgatt cgtctggaga acggcgtcat cgttcgtgcc 75600agcggtatca aacataagta cctggatgag atctggaaca atcaggagaa atatcgtgga 75660cgcatcgtcg aggttcactg ccatgagaaa acgccagacg gtagcttacg tcaccctcgt 75720ctgaagtggc cgaaatgtct gcgtgatacc gaagatcgta ttggagataa agactgatgt 75780tcggctggat gattgtattt ttggttgtcg gcattgttat cggcagtctg gttatgtcca 75840gctgcatcaa cgattacgta aaagccggtg tcatgcagag gcgcggccgc atttaccgca 75900ttgtagatat cacgtacaca ctgaaggaga ttaaggatga tcatgttaag taaacgggag 75960aaggaaaccc tgcgtgaaat cagccagtgg aaggagttct acgctaactg gaagccaaag 76020acccgcgcca aactggaacg catgaatctt gtcgctaacg tttcgccaaa gggatgcttg 76080gagaactatc aactcactga aaaaggacac tcactgttgc agcaattgac tgaggctggt 76140gcgttccaat gattccatac atcacattat cttttgctgg gggcgtggcc ctcggcttca 76200gtatctgtcg cgatctggtc aggcaggaac tgaaaaccaa aacgcttcgc atcggtaagc 76260gtctgtatcg ggttgttcac gagacaggag tgcagaaatg agcaatttga cctctttcga 76320ctggtggctg gcaacctacc ttgtggcggc cggcttcgga tatgcctttt acgttggtca 76380gttaatcgtg aagctattgc tgatcagatt tgccagccat aaacgcatag atgacggtct 76440gtggcgcctg ggttctctgc tggagactca ctacggcgaa ctcaaggaga acgaaactat 76500cactattcaa gcgaagcgtt ttaccgccat catcacgaga acgccggaac agaaggtgag 76560tttgatcaaa aagatagcaa ctgaacgagt cacagacaaa taagtattta cttacttatc 76620tattatgtat aagattcatt tgttttcgtt gggacgcgac tgtttgaacg ttaaatataa 76680ctgcaaacga agatacgtac ctggcagtag cctaagaagc caaacaccag cgaggtcagt 76740ttccagcctc gtcaccgaaa tgggacacac tgagcgagtg tgattgcaga acgcaggata 76800gggcatgttg caccacccat gccctattcg atgaagtaac aggatgggcg gttggtttcc 76860tcattccatt ccatcatccc ggtttcagcc agctgaccgt ccatcctgtt acgtcatttc 76920cattacttat gtcgtttaat cttgggttaa aagcggcgac gtaacccggc tggctcggtt 76980agccagcgca caacgttgag gtcactgttt tttcttttaa tcatacaggt gattccacag 77040aactgtagtg actgatcaat atgttgggtc gaacataaat cggttcagtg gcctcaacgc 77100tgtgaaatcc aaaataatta actctttggt aatcctaatg ttactgacat ttccggcttc 77160acaatattat tgtgatatac ctgcatttca ttgtaaaact ccatgtcatc acaataaatc 77220gggagaaaat aatgacaatc aatagtattg tcatttcgtc tttttatgac ctccacatcg 77280taaccatccc gcttatttaa attataaata ggcaaagagt tataatatat gttgtacatg 77340tgatgaacat actgatcata tgtatcattg aagaaaaaat aagcatacag gcctttatct 77400acctttattt cactatcgct gaaatgctta ttcctatccc aaacaaccgt tcttgcaata 77460atgtcattcg ttttatcatg aaaggggatt ctgtttgata ctgttatagc tgtatttttc 77520ttcaacgaat catataactt acccaatcta cattttgaat ctatcccaga gtagaacact 77580aaatccctaa aattaaagca ttgaccaatt atatttctct ctttaaggta acagattcgt 77640ggttgaaggt attcacagtt aatttccctt ctacccaaca aacctttaaa ggaccaaaaa 77700gggatcatcc tatactgccg tggggtataa ccaaatattt tcttaaattc tctggtgaat 77760gtctgttgcg aatcataaaa aagctttgct gatatctcta ttattgtcag cctggtaagc 77820cgtaatagtg cagcagctct actagccctt ctaactctaa tatatgttcc aataggcatt 77880ccgacatatt ccttaaagga aatttgcaaa taccttctgc tgaatcctga atacaaaacc 77940aaacagtcaa tgttaatgaa tttcgactcg agattctctt ctatatattg aataattgaa 78000tttacagtca tctgttttag catgaatata acccaaatca aaataatagc attctagata 78060gtgggccggc gccgggactt agctatttgc gcatacccag caacaccaat cttagctatt 78120tgtgcacgcg catcaatatc aaaattagct atttgcgcaa caagcaagtg gagtgcgcga 78180aaagctaaac tttgtgtgca tttttaaata aaattgttct cagtgaggct gtgctacgga 78240tataaaaatc cccttcattt gttacccacc tttttacgca tatcgtcgat atgaaatgat 78300ggggaggggg tgggaaggtg ttgtcaccat tccgtaagga ggttaagctc atgattttaa 78360atagattaag tacgttagga attattactt tcggcatgct tagttttgct gcgaactctg 78420ctcaaccaga tatcaaattc gcaagcaaag agtatggcgt gactataggt gagagtagga 78480tcatataccc gttagatgct gctggcgtta tggtctcggt gaaaaacacc caagattatc 78540cggttctcat tcagtctagg atctacgacg agaataaaga aaaagaatca gaggatcctt 78600tcgtggtcac tccgccattg tttcgattgg atgctaagca acaaaattct ttgcgtatag 78660ctcaggctgg aggtgttttc ccgcgagata aagagagcct aaagtggtta tgcgtaaaag 78720ggattccacc aaaggatgaa gatatatggg ttgatgatgc gacaaataag caaaaattca 78780atccagacaa agatgtggga gtgttcgtgc aattcgcaat taataattgc attaagcttt 78840tggttcgacc gaatgaatta aaaggaaccc ctatacagtt tgctgaaaac ttaagctgga 78900aagttgatgg ggggaagcta attgctgaaa acccctcacc tttctacatg aacataggtg 78960aattaacatt tggagggaaa agtattcctt ctcactatat tccacctaaa tcgacgtggg 79020cttttgattt gccaaaagga ctagcgggag cacgtaatgt ttcgtggaga ataattaatg 79080atcagggagg gttggatcgt ttgtattcca aaaatgtgac tttatgatga tgtttaaagg 79140ggacgggaat aatgaggtat tcaaagctgt tcctgtgtgc agggttaact ttggcaacat 79200tgccttgttg gggacgcgca tatacttttg actctactat gcttgatacg aatagtggag 79260agagtataga tgtatctctt tttaatcaag gacttcaact tccaggtaat tattttgtta 79320atgtttttgt aaatggtcga aaggtagact ctggaaatat cgacttccgt ctagaaaaac 79380ataatggaaa agaacttctt tggccatgcc tatcatcctt acaattgaca aagtatggca 79440ttgatataga taaatatcct gatttaataa aatctggtac agagcaatgt gttgatttat 79500tagcaatacc acattcagat gtgcagtttt attttaatca gcagaaatta tcgttaattg 79560tgccaccaca ggcactttta cctagatttg atggcattat gccaatgcaa ttgtgggatg 79620acggcattcc tgctctgttc atgaattata atacgaacat gcagacaaga aaattcagag 79680aaggaggcaa gtctctggac tcttattatg ctcagttgca accgggatta aacatagggg 79740cttggcgctt tcgtagttca acctcatggt ggaaacaaca aggatggcag cgttcgtata 79800tttatgccga gcgaggattg aatacaatta agagccgttt gacattgggg gaaacctatt 79860ctgatagcag tatctttgac agtatcccga ttaaggggat aaaaattgct tcagatgaat 79920cgatggttcc ttattaccaa tggaattttg ctccagttgt tcgcggtatc gcacgtacac 79980aagccagggt agaggtttta agagatggct acactgtaag taatgagttg gtgccctcgg 80040gaccatttga gttagcaaat cttcctctgg gtggggggag tggtgagctg aaagtcatca 80100ttcatgaaag tgatggaaca aagcaagttt ttacagttcc atatgacaca ccagcagtgg 80160cattacggaa gggctatttc gaatattcaa tgatgggggg agaatatcgt ccagctaatg 80220atcttacaca aacatcgtat gttggcgctc ttgggatgaa atatggtttg ccaaggaatc 80280ttacgttata tggtggacta caagggtccc aaaattatca tgccgcagct ctgggtatcg 80340gtgctatgtt gggtgatttt ggtgccatat ctacagatgt tactcaagca gacagccaga 80400aaaataaaca aaaaaaagaa agcggccaac gttggcgcgt tcgatataat aagtacttgc 80460agagtggaac atcgttaaac attgctagcg aggaatacgc cacagaagga tttaacaaac 80520tcgctgacac gttaaatact tattgtaaac ctaatactag aaacgattgc cgttttgatt 80580atgctaaacc caaaaacaaa gtgcaattca atttaagtca aagcatacct ggttcgggga 80640cgcttaattt cagtggctac agaaaaaact attggcgtga cagtaggagc acaacttctt 80700tttctgtagg ctataaccat ttttttagga atggtatgtc attgacttta aatttatcga 80760agacacagaa tatcaataag tatggagaaa aaactagtga gctattatct aatatctggt 80820tgagttttcc tctcagtcgc tggctaggta ataactcaat aaattcaaat taccaaatga 80880catcagattc tcatggtaac actacccatg aggtaggtgt gtacggtgaa gcctttgatc 80940gccaattata ctgggacgtt cgcgaacgtt ttaatgaaaa gggcagaaaa tatacctcca 81000atgcactgaa tttgaattat cgaggaactt atggggagat cagtggtaac tacagctacg 81060atcaaaccca aagccaactt ggtataggtg taaatggcaa tatggtaata actcagtacg 81120gtataacggc tggccaaaaa actggagata ctattgcatt agtacaagcc cctgatataa 81180gcggtgcttc agtgggatac tggccaggca tgaaaacaga ctttaggggg tacaccaatt 81240atggttactt aaccccttac agagagaata aggtagaaat taacccagtt actttaccca 81300atgatgcaga gataacaaat aatattgtta gcgtgatccc gacaaaggga gctgtagtat 81360tagcaaaatt taacgcaagg attggtggac gattgttttt acatttaaaa cgctctgaca 81420ataaacctgt tccatttggt tctatagtta ccattgaagg gcaatcatcc agctctggca 81480ttgtcggaga taatagcggt gtctatttga ctggactacc taaaaaatca aaaatacttg 81540ttaagtgggg gagagataaa aatcaatcat gttcatctaa tgtagttcta ccagaaaaaa 81600cggatatttc tggtgcttat aggttatcca caacctgcat cttaaataac tgaaacggat 81660gtttatttca aacaggacac aagccctctc tacgaatttg ttcgtggatt ggattattcg 81720atagaggtaa tatatgaaaa aaatcagttc cgttatcgcc attgcattat ttggaactat 81780tgcaactgct aatgcggcag atttaactgc aagcaccact gcaacggcaa ctcttgttga 81840accagcccgc atcactctta catataagga aggcgctcca attacaatta tggacaatgg 81900aaacatcgat acagaattac ttgttggtac gcttactctt ggcggctata aaacaggaac 81960cactagcaca tctgttaact ttacagatgc cgcgggtgat cccatgtact taacatttac 82020ttctcaggat ggaaataacc accaattcac tacaaaagtg attggcaagg attctagaga 82080ttttgatatc tctcctaagg taaacggtga gaaccttgtg ggggatgacg tcgtcttggc 82140tacgggcagc caggatttct ttgttcgctc aattggttcc aaaggcggta aacttgcagc 82200aggtaaatac actgatgctg taaccgtaac cgtatctaac caataatcca tatagataat 82260agataaagga gggctattat gccctccttt aatatttatg aattatccta ctttgagcct 82320aaccctcgct tttcttaatc acggcattga tagcaagact gacaaattta tgtgaagatc 82380aatgttagga actaatgcag aaagccacgc cctcaataga tttcacataa tacactatta 82440gctaagaata gagagcgcga agcaatataa taggctcata tttataactc tcaccttaat 82500atgcacttgc aataccattg agtatattat ctggtgggaa tttttttctc ctaagcgagg 82560atatggtgtt ccaaaagcga tttcaacata tccgcctaac tgcgggaaga attattgttt 82620taggtgtggc aattatttta atggaaatct tgttgttagg gattttgttt tttattagcg 82680aaataatccg aaatttataa tcaccctccc ttttgcgtca tttctttcag gcgccctcac 82740cctcttgagg agagcaagag gggtatccat catccatgca acttcaacaa agcagccttt 82800gccctcaccc cggggttttt ttatttggta gcaacccacg tcctgcagcc ctcaaaacct 82860tcatagccgg taaaaatgat tccataccta ttaagtatgg aatcattgta caaaacaggc 82920tattttatgg ttgatcatac aaaaacttat gcctaatata ctgttcatgc atacagttaa 82980aagggcttca ctcagtgagt ggacgatgaa aaacaccttt gataaagcac gcgcagcaga 83040aaatacctct cgggaagcca tcgagtacct cgaacgcgct tccggtttgt cggcagtgtc 83100gaccgccaat ttcgacggag acatgtcgtt ttcttccgcc ttcatgttgt tcacccgctt 83160atccttactg ataacgagac gtcgacctga aattgccgtt cattgtgttt tgatacatgt 83220gatgccgcat atctctgaag taaaggtaag tgatataagt agggtcttgg tcaaccagct 83280ggtgaaccca ctgatactgg aaggcaagat cgtccagggc agacgcgtgt tctccctgat 83340gaaacagttc ctgagctggt gtgcctttca gggattgatt gatacttcac ctttgaatga 83400tatgtcgctc aacaaagttg ccggtggcgc aaagccagta ccgcgcgaac gtaagctgac 83460cgacgctgaa gtctgggtat tctggaatat ctgggattat ttcaacgtat gtgaaggtac 83520aaagtgggcg gcaaggctat gtcttgttgc tgccaggcgt ccagatgaag tgctccgggc 83580cagaaaggat gagttcaacc ttcaacgtga tgtatggaat caaggtacgc gaaacaaatc 83640ggccagacag cacgcgctgc cgctaagtcc attaatgcgc aaatgcgtgg aagagttgtt 83700cgaatacgga aagggcagcc agtggcttgt tccgtcaaat aagaagaagg gcgttgatac 83760accaatgtcg aaggtggcca ttgcacaagc gttgagaaga attctggagc gcccagagct 83820gatggatttg gaacctttca caccacgaga cctgagacgt accgctcgta gttattttcc 83880agccctgggg atcaatcaag aagtggctcg taaaataatg aatcacagtt tggaaggcat 83940agatcgcgtg tacgaccggc acgactatat ggatgagatg cgagacgcct tggatagatt 84000ctcagcgtac atcgcatcca tcgtagagca acaagattta gacgaaattg accacaaatt 84060taagggagat cgtctagcaa ctgagcttat tcgtgtaaat ttctcatagt ttcttgattg 84120cttcaacaac ctgatctgat gcgccgcgtt gatcaccgaa acggttgcga aacgcttcta 84180atactctttt ttcgtctgcg gaaaggggag ccgttccttc ttcgcggaag aactcaagca 84240actccgggtg acgctcttcc aggaccatca gcattagccg aacagggtca gcgtccagag 84300ctacggccag cgctcttacc ttatcaacgg ggagagggat ctttccgctt ttgatgagag 84360acaggttgtt ggcgttttta taccctactt ccttggcaat agtagcctga cttttcggtg 84420agatagtgat cagggaatca atataagccg cgtagcgacc ttgtttgcta tctgtttcgt 84480tggtagccat gtaataacct tgcgtgttta atttgtctct ggtaagtgct tactgatatt 84540acattaaagg tgagggttgt aaagacttat ctatttcttt cgataggcat caaaaccctt 84600tacctatgcg cattatacat ctattaagcg gcaaattgtc attatcagct tgcatggttt 84660gtcagacaaa tctgatatag atgtattccg tattgataca ataggtagta gtatttccga 84720actttcgaac gtgttgagtt ggatgatatg agatgaaaaa tataacttct aatttgaacg 84780cccttgaggt tggacatgca tatgcgctcg ggatggatgg cgtggcaacg atacttacag 84840aacttaatac tgaagaactt ccgatagaca tgagcgacac gactgtcttc actttcgaac 84900tcagcaacaa acatttcact ctcatcaata ctggctgcgg ctcacttgcc gtaagaaccc 84960attaactgca aatcctacct attgaaacct gtacgaacag cttggccacc tgttcgtaca 85020gcataaaatt acacacatta gaaaacaaat tgttttaata acaaggaaat tctcatgtcc 85080aaagccatga ccagagctgt gctgaaagag gtacaggact tccgcgactg cgtaaaacga 85140gttgtagcga tgctttcagg caaacagata cctgttgcag aacgaggcaa tgaagcgtat 85200gtccgctaca accggcgcgg cgaaccagtg ctggtaaaca tcccatctat accggacgac 85260gcatcaccaa ccctcatgaa cgcagtacgt ggttttctcg accatgaagt agcccatatc 85320ctctttactg atccgaaagt cgcgatgaag atgcgcgaga gaggaaaggc tccgtctacc 85380gggctttgga acgcgctgga agacgttttt attgaacgca gaatgggaca ggtattcaac 85440ggaacccgtc gtaatttgct agccacacag aacctggtga tcgacaaata cttcaagggc 85500aaagtgtcag aggcggtttc aatctgccac ggcaaccagc gtgaattgtt cctgaaattc 85560ttcctttgcc cggtcgtccg cgcctgggat ggccaaagcc ctttcatcga ctttatggaa 85620gaacactggc acctcatcga taagcctgta gccttgctca aagagcatgg tatcgacgtg 85680gccgttcgca atatgtcgaa cacagaggac tgcgtaaagg tcgcagctgc tatcgcccaa 85740atcatgcagg atatgaaaga caagccagag ggcaaattac ctgagcttaa atcatctgcc 85800aaaaagccgt ctaagagcaa agacgagtcc gaggaaacac cagagtcagg cgacgatccg 85860tctcatagtg aatctgcacc aaagcgaacc aaaggcgaag acgacgacaa ggaagagcaa 85920gaagatgatg cctcagaaga ggaagattct ggggattctg atttgcctga ttcattagat 85980aaggacttac ctatacatga taaagaaatt agtgatacag aaagtaaata tacagaagca 86040ggcgaagatg agtcaggaga caccccagaa tccgatgatg ccggcatgga atcaggtgat 86100tctgacgacg aaggtggtag tgatggcact ggcgctccaa cgcctggtga tggcattcgc 86160gaagacgccg atgactccga tggttatggc tctggcgccg ctggcgatgg ggatagtgat 86220aacggcgaag actccgatgc cggtcgtgag gagtccgaag gagaagacga aggcgaagag 86280gacgtcgccg atcacactga cggcgaaggc aaggaaaatg aggatgccgc ggaagcgcct 86340gaagacagtg agtcaggctt tgttcctgcg ccggatgaaa tgactctgga ggacgcactc 86400aaggcgctcg acgagatgga agaaggaaca gacgaaatga ccgaagacgc actgtcggcc 86460accatcagca aagagcttat gagcgcctca ctttctgagt atcgcccata cgatcgttca 86520tacgacttta tcgggttgat tgatgaggct gaagagcatg taaaacgcac cagaaagaca 86580ttcggcgcaa tcccaatgca ctcacctgtc gatcgctacc gcatggttcc ggaaggcaga 86640aaactctttg aactgaaaat cgaaaaacat ctgtctgccg gcgtttcttc gactctggcc 86700aaagacctgg agcgagctat cgccagccgc aaccgagttc agttcatacc gggtcagaga 86760cgtgggcgga tacatggcgc gaacctgtat cgtctggcaa tgaacgacga ccgcgtgttc 86820cgcaagaaag aagaccacag agcggtgaac gcgtgcgtcc agcaggtgat cgacttgtcc 86880ggctcaatgg gcggcaggaa gattcaactg gcactcgcca gcgcctacac cattgcggat 86940gctctggatc gtatcaatgt gcctaacatc atcaccggct ttacaacgtt tggtagccca 87000gattatgaaa ccatgtcgaa gcgcgggttt acacgtttcg aggcgctcat gctgcccatt 87060atcaaaaact ggaatgagaa agctaactca ccagagatcc gcgcccgcat gggctgcgta 87120tgtgagacgt tccccctgct caataacgtc gatggtgaga gcgtcgcgca gctggccact 87180ctgtttgcag ggcgaatgga ggacaaaaaa atcatgctgg ttatgagcga cggggcacca 87240tgtgctgcag gcgatgggtt ccatgagcat ttgcgaaccg tcaccaaaga aattgagacg 87300ttaagtgaca tcgatttgat ggctatcggt gttctgactg acgcaccacg acgctactac 87360aaaaattacg cactggttaa cagcgtagaa gagttagggc cgtcagtcgt cactgagcta 87420tctcgtatca ttcttgggta agaacttcac ccttaaaaaa taagtaatta cttactatac 87480agtctaatat atttatataa gatatacccc acgaacgaca aacagtaagg aaaaacacat 87540gaccgctact gcactacagc aagaagaaca tttgccggaa gccatcgtct gcaagtggtg 87600tggcaaatct tttcattacc tgaaatccca tatctctatg ggccgttgcg agaacattcc 87660tgagtccgcg aagggtctgg acgtggacga agtggtgaaa atgtacacct ctgcgtttcc 87720agatgaacca acgatctctc gcacggcact ggtcaaactc aatgagaagc gtgccgaaag 87780acattcagga gaaggaaaag tagcggagat tagcgcacat ccgggctacg caggaacggt 87840cgaatacaag accgaactgg tggccgcgca cgagctgctt ggcgtaacga tcaaagagct 87900gggaacgcca cgcggaaaac cactacaggt gacggttaac gtcaacacac cctatccgga 87960gttcgtgcct gaagcgaaga agaactatgt gtatggcgac tttgacctga ttaaagacat 88020cttcatgatg ctggaaatcg gaatcccagg ctatctctgg ggtcatgcag gaaccggtaa 88080atcttctctt cctacgcagc tatgcgccct gctgaatcga ccactgatcc gcgcccagca 88140tacggcgtct atggaagaag cacacgttac aggccagatc ctcgctcgcg atggctccac 88200ctacttcgag ccgggtttgc tggcgctggc gatgaaaaat ggctgggtgt acctagctga 88260tgaatatgac ttcgcgttcc cgcagattct gggtgtgtac cagccagttc ttgaaggaga 88320accgctgatc atcaaagagg caactccgga ctggcgccgt atcactccgc ataaacgctt 88380tgccttcatt ggcactggca acactaacgg ctctggcgac gaaacgggtc tctatcaagg 88440tacgaacatc cagaacgcgg cgaacttctc gcgcttcggc attgtttcga acgtgaagta 88500catgagcact aaggctgaag tcaacatgct ggctgaggct ggcgtcatcc gcgaatacgc 88560cgagaagatg gtgaaattcg cgaaccttgt ccgtgaaggt tatgagcagc atctgatcag 88620ccagccaatc ggtcctcgcg agctgctgct atccgcaaaa atcggaatga tgcgcggtga 88680tttctctgcc ggcatcgaaa agtcatttat caataaactt ccatccacgt ctgcacaggc 88740agcgcgtgaa gtggttcaga agatcttcgg ttaatcgtgc gtaaaggatg tttcggctct 88800cttatcgcag cgtctgaaac tggccgggct tgtctggcgt gtccggatag gtccgagtgc 88860caccagtcag ccaaagaggt tgcgatttcg atgtatggga agttcgttgg cttccccaat 88920gacaaaatca agaaaaccag aaaggtaaaa acacatgaag gctctgatgg tcagaactga 88980cttctccctg ggagagtcag ctctaaaagc agaaaacgcg gtgaaaatcg cgagagacgc 89040tggctacact gctgtcattt ccgctgacag catgaacatt gccagtgtga ttcccctgca 89100gcgtgccgct ggcgacgata tggcggttat ttgtggtgtt aagctgaatg tggtcgacga 89160tccgacatac gagcaccgcg cccgccttgc gaaagaatca gagagatgta tggaatcatt 89220ggtgcgtgat cgcagctact gcttcacggc actgataaag aatgagcaag gttatcgcga 89280cgtgtgcgaa ctgatgacct tagcgaacaa gcgcgagcaa ttctactttg tcccgcgtct 89340ggcgctcgac caactggcgg ccgcgtatgc caaaggcaac atcatcctgc tgacgtccga 89400cattggcagt gtattccagc gccgggactt cgcaaagatt atcgggacgc tggtcacagc 89460tggaggacgc gataacttct acagcgtggt ttatccgcac cctaccccat tctacgacca 89520gattaacgtc cgggcgatga aagtagcgag cgcactgaaa atagagccag tggcgttcta 89580tcccgcttat tacgaagcgg tcgacgacgc tgacattaaa gacattgcgc acatggttac 89640gaacaacatc aaaatcgacc agccgcatcg tctgcgtatt ccccaccagc gagataacgc 89700cgttaatggt cgccgccatc tccttgaagc gctgaaagcc ttctccgttc gcatggatgt 89760accggtaaca gctgcaatgg cctcaacaac gcaggacacc attatcgaag cctgcacatg 89820gcgctggcat gaattgccac cagcactgcc caagatggca gacgacgagc ctgcaacgct 89880gatgaagctg gctgtcgcgg ggctgcgcaa gcgtcttact accaaagagt ttggctacac 89940accaccggct tctgagcacc gcgtgtatgt tgatcgtctg aagtacgaaa tggacacgct 90000gacccgtctg ggcttctgtg gctacttcct gatggtgcgc gacctgatga atcacagccg 90060tgaaactggc attcctgtcg ggccaggtcg tggttcctct gccggttctc tggtggcgtg 90120gtgcatcggc ataaccaacg tcgatcctat ccgtcacggt cttctgtttg agcgtttcat 90180caaccctgaa cgtctcgact taccggatgc ggatctggac ttcagccagg cacgtcgcca 90240tgaggtgatc gagtatctga atgaacgcta tggcgaagat tacgttgccg gtattccgaa 90300cttcacctat ctgggcgcgg cttctgcgct gcgtgacacc gcacgtattt acggtgtcga 90360tgctgcggat atggcggtat ccaaagagtt caagaatctg gaggacgata gcctgtcgct 90420ggaagagctg cgcgagcaac tggccagcct ggacaaatac gccacgaaat acccggaagc 90480gttcaaagcg gcgtgtaagc tgcaaagcct gatgcgtggt tttggtcgcc acgctgcggg 90540gatgatcgtc gctggcgttc cactggtaga gcgcacgccc gtcgagctgc gtggcaatgc 90600tcgctgtatt gcattcgata aacgttactg cgaggcgatg gggctgatta agctggacgt 90660tctcggtctg gcaacgctcg atctgttgga tagcgcgaaa cgctacatca aagagagtac 90720cggggaagac atcaatctcg atgctatccc actggacgat cgtaaggttc tggatgggtt 90780cgctgcaggg tacacgcagg gcgtattcca gctggagtcc ggtcccatgc gcaagctgct 90840taaagatctg ggcggtggca tcgagccaat gagcttcaaa accgttgtcg ccacgaccgc 90900actcttccga cctggcccga ttcaatccgg catgttggac gactatgtct ccgtggccaa 90960aggcttcatg gctccacagt cgctgcaccc ggtactggac gagcttaccg cggaaaccaa 91020cggcgtgatt ctgtatcagg aacagacgat gaacgcgaca cgattgctgg ccggtttcac 91080gatggccgaa gcagatggtg tacgtaaagc gatcggtaaa aaggatatgg aaaagatgaa 91140gagcatgggc gagaagttcg tcgttcaggc tcaagctggt tggatcgacg ttgagatgga 91200agacggcacc acgcagcgta ttcaccgcgc ggaacacttc aaatgtgagg acggcgcact 91260gcggacggtc gaagaagcgc tggaggcagg tgtgaaattg ccgatggctg ctgtacgcgt 91320tacagggtca caaccgggct tgtctgagac gaaagctaag gagatctggg atgccttcga 91380gaagaacggt gcgtatcagt tcaacaaatc acatcccgtt gcctactcgc tgatcagcta 91440tcagtctatg tggttaaaga cacattaccc tgctgagttt ttcgctgctg cgctcaccat 91500tctgggcgag gataagcacc aggggctggt taaggatgcg ctgacctatg gcattcacgt 91560attgccacca gacgttaacg tgtcatctaa ccgaattgag atccgcacgc tggaagacgg 91620cagccaggtt ctgtatgcgc cattctctgc tgtaaaaggc tgctctgaga atggttgcca 91680ggccatcatg agagcgcgtg agaaagttgg cggcaaattc gagtcacttg agcaatttga 91740ggaagcggtc gagaagcgtg cgtgtgcgtg taacagccgg gtacgcgagt cactgcaaaa 91800agtaggtgcg ttcgcatcga ttgagcctgg cagtctgcca gcgacagatc cggaacggct 91860gcgcgaccag gctgaattga tgggcaatct ggtgatcgac gctgtaaaag cctctcgacc 91920gttcgagatg aaccctaaac gctctgccga agtgaatgta ctgatgacac gcatggcggc 91980tgaaatgggt ctgggagacg acctgatacg cccaagcatt ggcattaagc cgaaaatcat 92040ggtcattctg gacaacgcga acggcaatga tgggcgtact ggctacttca tggagaacgg 92100ctacgacgac tttaaggcga agttgcttac tgcaggcgat ttgcgcatgg gagatctcta 92160cgtcaccggc gtgtgcaaaa aggtgaagga caaagagaag gactacacca aagacgagat 92220aggccagttc accgacttta tgcgtgaaga gatcaatctg gtgcgtccga cctatgtgct 92280gacgtgtggc agccgggcga cgtcactctt caacaacaag agcaaaccat ccgacctggt 92340tggacgcaaa gagtatctgc cagagctgga tgtgaccgtt ttctacggat ttaacccgaa 92400cattttgtac tttcgcccag aggaaggcga aaagctggaa gcaattctgg cagaggtagc 92460ggagacaatt agcaaatgaa taaagagaac accatgaacg aagcacagaa gattgcacaa 92520gcgctggcgg ctatcccagc ggattttcag gataaagcag ttgcagccac catgcggtcg 92580cagttctggg aaatcatcga ctgcccggtc acgttagatc tggcgctggc gttcgccggg 92640ctggatggtg ccgataaagt cagtcgtctg cgtaaatgtg ccagagcgct ggcgcttaaa 92700acgcaagatc cgaaggcgtg ccagtatctg ctggagattt acgaatcgga taacccagag 92760gaacagctgg aggcgttcaa agtgttccgc aatcggctgg tgctgaaggt ggccaaagag 92820tttatggaag tgaacaagat tggcgatgtg agacagtaca ggctgaaacg ccagaccaga 92880gtcacgctat ccaacatttt tggtaagaaa gtcgcataaa gcaaaaaccc gccaatcggc 92940gggttttttc atgcgcattc ggcgtgatga cgtcgacgtg cgataagtga agaagcgata 93000tgttcaatct caacaaagtc ttttgagacg ctattgcgaa gggccaaatt ccacttactt 93060aatacgcgag cattgtgtgc cagctctgcg ttttctttaa ggcgtccatt ggcttcaagc 93120cagttcgcca catcagccca atcccatagc ggggactgtc cctggattct ctggataggg 93180caagggaagt cgccaccacc acgaagccca tctttaagca tggtaattgc ttgtcgggac 93240atgcccgtca tttcagctac atcgctcagg ccaactaagg ccgagtcgac tgattctaca 93300atcgcgccga taccggcaga ttcgatattg tcgaccgctg atgcaatggc tgcatccagc 93360gatttggctt cgcggtcgaa ctcaacatag acggagtttc catatgcgca aacaatcgca 93420tcgccacagc cgctttcgta cagcgcatct tccaatcctt cggtctcata ggttacgcct 93480gagagagtca gagtgaagtt ataaagcgcc ataaaacctc tttatctaaa gtgaagtatt 93540aatgtacctt tggaacaaat ggcggccaaa accgccacta actactattt tttgctcatc 93600tggtttttga tgcgaccaca ctggtcgacc gcttgcctga tttgcgtggc atggtgctcc 93660ggtacatctg gagtcgacca tacacttctg tggtgacttg tatgttcacc tgatttatcg 93720ccgcagcgca gcttgcagaa gcaatgtgct gacttacctg ctggaaccca aacccagcct 93780ttactcaacg cgtattcaat ggcctcttga atatgcttat tcggatgtga tttcattttc 93840ctccgatgtc attatgatag gtatagtgtg agcgcgtgtc aacactgtct attttctgac 93900gtctacggcc gcagaaaacg gctatagatt atcagaacac tccctgtttt catcaactta 93960ccgaccccac cccctaactt cttccttcta taagctccat ccttcatttc tttcatggta 94020aaattgatat atagaaataa gctggaacat atcaaaatga gtgcagatat ctacgaaaaa 94080atcatgtccg atctggagtt cgaccgcgac aatcttgagg aagtctggcg tcagcaaccg 94140cgcctgttga tggagtacgg ctctaagctg gcgcgggcag aacgcgaggt cgcagatgca 94200aaactctccc tcgatgcgat tgaggcaaaa atctactaca atgagcgtaa gaacctgagt 94260atgaacggca ttaagttcaa tgaatccgta ctggaggcga aggttagaac caacccgcaa 94320tacctcgcaa agcgccagaa actcgatgat gcccggcaca ttgcagatct atacaagcac 94380gctgtaaccg ccttctctca ccgccgtgac atgattgtcc aggcgtccaa aatggctatc 94440gtggagattg aacgcttggg cgccgaacgt ttccactcgc cccgttaatt tatcctagat 94500gataagtaag tactgatcta ttatccttct cgctcgaaag agccacgaat aaacgaatgc 94560ccaacgcgca tagcgccaat ggccacaatc acaaaaagga gaaatacatg tctaagtcat 94620tacttgatct gcttaacaag acccgtggcg atattgcttc taaacgtggc aataacgttg 94680atttgacccg tctgaaagac ggcaataact atctgcgcat ttttccgaac aaggacgacc 94740cgaatggtgt gttcttccag actttcggta tgcactacgt taagcatcag aatgaggaag 94800gcaaagatgt aaccaccgcc tacatctgcg aacagcacac ccacggccac gcttgccagc 94860tgtgtgagat ggttatggaa ggtcgtgctc gctttaaggg caacaaagcg atggaagagc 94920gcattaacag tatgcgtgct acaccgcgtt atctggtcaa tggtgttctg tctgcgcgag 94980aagactttgc agacgcagag aaatgccagt tgattgagct gccgtctacg gtcttcgacg 95040atatctgcaa agtgatgtcc gaagatattg ctgatgatat cggcaaccca ctgagcaaag 95100aagaaggcta tgcgttcctg attaagcgta ccggttccgg tcgtgacacc aagtatgacg 95160tatccccgaa acgtaaagtc tacaaaggcg acattcctga gaagctctgg actacccaac 95220acgatctgat cgcatacgcg aaccaggctg acgaaacccg tctgctgtct acggctcgca 95280ctatgggtcg tctgattggt attgcggctc cggcagcaac aatgtcctct ccggccattt 95340cttccgctgc aaaatcagct gctgctgaac tgccaggctt tggctctatc actggtcata 95400cggaaggcgc agctgctgtc gctacagcac acacaccggc tccagagtcc accagcctgg 95460ttgatgaaga gatcctgcgt gccgctgaag ctgagttcaa accggaaact aaaccggaaa 95520aggttaaagc tccggaagcc gccgcagctg caagtgcttc cgcatctgct gccgctgcat 95580ctgtaccagc tgacgaaggt ctcgatgacc tgctggctga actggacgct ctgtaatccc 95640ataacgtgac cagtaaggcg tctacggacg ccttactttt tggaaggagt gtaccggtga 95700attatctctt tgtggacggt aacagcctgg gctattacca ccagcaatcc gacaaattac 95760acaacggcga gatggaagtt caggcggctt ttggcttcgt gaagaacgtt cgtcgttacg 95820cctcaattct ccatgcccgc ccaatgatct tgtgggatgg attcagcgac aaacgtcgcg 95880acttctaccc ggagtacaaa gcgaatcgcg atgacgaccc ggatatgaag aagatgaaag 95940aaggttttgc catccagaag ccgtacatct tgaaaatgat gaccgcgctg ggcgttaacc 96000aactcattgc aaaggatgca gaagcggacg acctggcagg aatgctggtc tctcgcctgg 96060ctccgcagcc gaccgttgat catatctacc tgctgactgg cgatggcgac tggcttcagc 96120tggttcgcga gaatgtgagc tgggtaagcc tgcgtgaaga tgccaagcac aagcaggtga 96180acttcgaaca gttcgcagag ctgaccggtc tgccgacgcc acgcgcgttt ctggaagcga 96240aagcgttgca gggcgatacc tcggacaaca tcaaaggcgt aggtggcatt ggtgatggtg 96300gcgcgaaaga gctgcttcat gaatggggaa gtgtggccgc aatggtacgc ggcattaacg 96360acggctccat tgtcatcaac aaaggtcgct ataagacggc attcaacaag ctggcaaaga 96420acgccttcaa cgagaagaca ggctgccgga tgctcgaagc ctttaagcgc aacatgatgc 96480tgatgaacct tatcgacaca aaattcccac ccagcgaaat cgagtcgatt aaaggcgcac 96540gcgacatgaa cgccttcgaa cagatgtgtt gcgagctgaa tttccggtcg tttctggaag 96600atctggaagt gtttgttctg ccatttgaga ggtactgctg atgctgaaat ccatcattaa 96660tggcggggca actacgccaa ccatgctggc taaagagatt gtcttctgcc acggcgaaca 96720cgctgtggtg gcgctgccga acattctggg cgctgctggc atttctgcta ctgagcgtga 96780gttcgcgctg gtcagcgagc aggtcgtgaa gatcatcgct cgcgtcgcca aacacctgaa 96840ccacgacgca atcaagtttg acgaagccgc agcttcgaag cgaatcaacg aatcaaaagg 96900agcctaatca tggcaaaagg caaatccgca ctggcactgg cgctgaaaaa gaaaatcggc 96960agcaatgacg agattcagaa ggtctcccac tggattgact ccggtttccc tccactgaac 97020aaagccattt ccggacgtta cgacggtggt tttccgtgtg ggcgtatcgt tgaagtcttc 97080gggccaccaa gcgccggtaa aacctttttg gcgacggctg cgatggtatc agcacagaaa 97140caggatggtc tggccgtatt ccttgaccac gaaaacagct tcgacgttgg tcttgcggtg 97200gcgaatggct tgaacgccga cgaagacgac ggtcagtggg tctacaaaca gccggatacc 97260ttcgaagact ccgttgagct gatcggcaca atcctcaagc tggtgcgcga cgaagagctt 97320atcccggaaa cagcccctat ctgcatcgtt gccgactctc tggcgtcgat ggtaccgaac 97380tcgaaagctg agaagttcga caagatggca gaaggcactg cgaaggacaa agatcagctg 97440aacatgaacg acaacacggc gctggcgcgc gcgacgagtg cgaacttccc tactctggcg 97500ctttgggcgc gtaagtacaa cgcgtgcatt atcttcttaa accaggtgcg taccaaaatt 97560ggcgtgatgt ttggcgatcc gactacgtct ccgggcggcg actctccgaa gttctacgcg 97620tcggtgcgca tccgtctggg agcatccgtc atgaaggatg gcaaagagaa gatcggacag 97680gacgttggcg ccgagtgcat taaaaacaaa gtcgcgcctc cgtttggtaa atgctcatgg 97740aaattctact tcgacccgac tcgcgggctg gacgtcatcg aatctctggt tgagtacatg 97800ctggaagaag gatacctgcc aaagaacgcc agcgggcgtg tggaaattag cgataagaga 97860tataccaaat cgcagatcgt cgagatgtac cgcgagaagc cactcccgga aatcatcgca 97920gcactccagg cgatagacga acggcgagcg aaagagtcgt ccccagcaga gacagaagaa 97980gcgtaatcac aaggcgccca ttgggcgcct ttttatactt gaaaatatat aagtacttac 98040ttattatttc tgcaccaaaa cgacaaaagg aaacacatga ttaagggtta tctcatggct 98100gtttcagcgg tggtgtcagt ctgctttatc tacggtttac tggttccatc gcttatctca 98160gctaaaagcg atctggcctt ctttatcgga cttgccatcg ctgtagtctt cccggttgcc 98220ttgttaaaag ctggccgcag gtatatcaac tcactcaata aaactaagga gaagtaagta 98280atgaagaaag gtttactggc ggtgactttg gctgctattt gcacaatggg tctgaccggc 98340tgcgatcgcg tggaaccggg atacgttggc attaaggtaa acaaattggg tgaagacaaa 98400ggtatcggtg aagttgtcgg cgttggccgt cagtggactg gtctgaatac cgagctgtac 98460accttcccca ctttcaaaca gatgaaaacc tacgatgagc cgttcacatt ccagatgagc 98520gacggtacag ccatcggcca caaaattggt gtggcatatc tggttaatcg caacaaagta 98580actaccgtat tccagaccta tcgcaaaggt gttgacgaca ttaccgacac tgatctgcgc 98640cagaagattg cggattcact gaaccgtctg gccagccgca tgactaccga cacgttcatc 98700gacggtggca aggcgtctct gctcgacaat gcgttgaaag acattcaggc agaaatgtca 98760ccggtaggta ttgaggttat tagcctgtca tgggtgggca aaccagacta cccggacacg 98820gtcatcgaat ctatcaatgc caaagtgacc gcgaaccaga aaacgctcca gcgccagcag 98880gaagttgagc agcgcaaggc agaagcgaac atgctgcgtg aacaagccga aggtgaagcc 98940gacgctatcc gcaaacgtgc tcaagcagaa gctgacgcca tcaagttgcg cggtgaagca 99000ttacgtcaga acccgaacgt catggagctg gaagccatca acaaatggaa tggccagttg 99060cctcagtaca tgactgaagg ggctaatact ccgtttatcg cgttgaagta acaccctttt 99120caaagatacg gcgtccactt ggacgccttt tttatttccg tattatcacc aacaagaaaa 99180caaattggtt actaatacgg aattaacttc cgttgaagta aaaagtgaca acgccacgca 99240atttctgaaa gaaggagatg atgaatgaaa aactacgctg aaatgacgga ctttgagatt 99300aactgcctgg tcgcggaagc aaccggccat cgccccctca tctcacaata tggctggaaa 99360ggctcacaag ttggagatta cacaaaagtg attgcgattg ggccaaacgg agcgggttct 99420ttcgactggt gcaacaatcc ggtagatgcc tgggacatca tttccagaaa cagaatcggc 99480atcattccag ccagacaggc tggcgagtgg agagcggccc acaggctggt ggatagctca 99540acaccacaac atctgatcca gaaccctaac cctttcagag cggcaatgat cgtgtttctt 99600ttgatgcagg agaaaaaacg tgaaaaaact gtatgacgcg gccaacgctg cgctggatgt 99660agtggatacc gaaattgccc agggcttccc ggagccagaa tgggcgacgc agctgcgtga 99720ggcgattgca gagatgaacg caccggaacc ttcagaagat gaagccgact ggcagcgttt 99780catcagaatg tacgcggaag agattggccc gacgccaacc gctgaacagg ccatgctgct 99840caagtacttc aaggaggctg gggagaatct gccggttgat gacacaccgc actggtttca 99900cgccgcctgg cgtaagttcg acgtgatcta cacccgcgat ctgggaagta aagatatggt 99960cgtctggcat ctgatgcaca ttgataaggc tgtcgaccgc acgctggaga agttctttcc 100020accagcctga acacaatgat tgtgtaccgc atggcgcaca ttagtataaa taagtactta 100080ccaacaagga gaagcacatg aagattttgg ttcgcatttc atcaagcacc gactatgacg 100140tttatccgtt gttcatggtc aagtgcgacg gtctgaacga tgaagaaatc caagcggcaa 100200ttgagcgcaa tctcgttgag tatactggta tggatgcgga ttctgtgcat gtcgatgatg 100260acggtgtttg ttggagcaat ggtagttgtt ggtatgtaga cgacacgacg ccggtaagcg 100320atgaagacgc tgctcacctt gagcgtattt taggcatcag cacttttgag tgatatttac 100380agcaaataat atataagtta gtatttacct atcatgaaaa ccgtattaga caccttatta 100440cttattatct cgatagcttt tgtgctcgat tgcatcttca ccggagtaat ccataaagcg 100500ctggcgcctg ttaatagcgc gatgattaat gcgctggccg tagtgctgtt attcgactca 100560gcattcggcg ttatccaagg agtcgtggca tgaagaaaac agccctggct ctggcactgt 100620tcactctccc tgtctacgcg aacacacatg tctatgagtg tgaaatgtct gtggccgaag 100680tgaaaaacga cgagatctgc aacgtcgtca aagctaacta cggcgcgatg attgtggaca 100740gcggcgaaca gttttatgtc gtgcgcgatg atcgcgtcct gtcttcaccc tatctcacca 100800gacgtaacgg caaactgtct ggcgtgggtg aagataagtt cgtttacgac aaatcaggtg 100860atgtttacgg cgttcacgcg aagaacgcca gctacctttt cgatgactgc aaggaggttg 100920gttgatggcg gttacaatgg caggtcttga aatcgaaaaa acaagcggct actggcgtgc 100980caaagggttt 100990 3 9960 DNA Yersinia pestis 3tggcgtgatg atggaactgc aacatcaacg actgatggcg ctcgccgggc agttgcaact 60ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag 120ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa 180acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga 240cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt 300catagaacgt aatgaaaata tcgtattact ggggccatca ggtgtgggga aaacccatct 360ggcaatagcg atgggctatg aagcagtccg tgcaggtatc aaagttcgct tcacaacagc 420agcagatctg ttacttcagt tatctacggc acaacgtcag ggccgttata aaacgacgct 480tcagcgtgga gtaatggccc cccgcctgct catcattgat gaaataggct atctgccgtt 540cagtcaggaa gaagcaaagc tgttcttcca ggtcatcgct aaacgttacg aaaagagcgc 600aatgatcctg acatccaatc tgccgttcgg gcagtgggat caaacgttcg ccggtgatgc 660agcactgacc tcagcgatgc tggaccgtat cttacaccac tcacatgtcg ttcaaatcaa 720aggagaaagc tatcgactca gacagaaacg aaaggccggg gttatagcag aagctaatcc 780tgagtaaaac ggtggatcaa tattgggccg ttggtggaga tataagtgga tcacttttca 840tccgtcgttg acaccctgat gaattcacgt gttcacgcct gaataacaag aatgccggag 900atacgcagtc atatttttta cacaattctc taatcccgac aaggtcgtag gtcgttatag 960gaaaattctt agcaccattc cggaacaatc agaacagcag gccatgaacg actgacaaca 1020ttacgaatat aaaaaacgca cccgggccag acattccccc tactgattaa accagccgga 1080cttgtccacg gaacggtctt tttaaaccga cacacagtct gagtacagat acatgtcacg 1140atgatgcagg attagcggaa gagtgtgagc acgtttccgg gaactgtggt gaaccatagc 1200tcaatattcg agtgagggca taccggaaac gcgctcagat tcgttgtaac gcgattttcc 1260gtaccgggca attttttcag ttgttttttc gtttcatgtc gtcagaaacg ttctgagcgc 1320gtttccggca tctgatgcta cgcaaaccat ccccatggtc agttgacagc cggaaacacg 1380cgggtgtcgt tttagcgtat cgacgggacg gcgtcgagaa gcacaaaaaa cagatgttgt 1440actcagtcag ttgttttaca gacagcactg cggcagattg aaaaagtacc gtactttcag 1500gaatgtccag aaaccatgtg tcagacttcg ttctccccct tccgggtgaa tttttttgtc 1560atccgttcag gaatctcttt ataacgatta ctccatttca ggatttttta tgtggcgttt 1620actacaggca ggatattcaa aggcaaaaaa atcccccgga acaggcggaa cccggacagg 1680gggagaacga atcgctaaat aattttcgta gttgtatttc ccatcgttgc tactgcaacg 1740ggatgaattt gccgcagttt atcctgtaaa acaatcctga tttactcaca ctccacatat 1800cactgacgga gcacaacgga atagtgaaca aacaacaaca aactgcgctg aatatggcgc 1860gatttatcag aagccagagc ctgatactgc ttgaaaaact ggatgctctg gatgccgacg 1920agcaggcggc catgtgtgaa cgactgcacg aactcgcgga agaactccag aacagcatcc 1980aggctcgctt tgaagccgaa agtgaaacag gaacataacg aagctcccgg agacggtcac 2040agcttgtctg tgaacggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggttt 2100tagcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 2160cttagtcatg cggcatcagt gcggattgta tgaaaagtgc accatgtacg gtgtgaaatg 2220ccgcacagat gcgtaaggag aacatgcaga tgccgatgct cttccgcttc ctcgctcact 2280gactcgctgc gctcggtcgt tcggctgcgg cgagcggtgt ctgctcactc aaaagcggtg 2340atactgttat ccacacaatc aggggataac gccggaaaga acatgtgagc aaaaaacgaa 2400gaccccagaa aaggccgcgc cggaggcgct ttttccatag gctccgcccc cctgacgagc 2460atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggactt aaagatacca 2520ggcgtttccc cccggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 2580atacctctcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgttg 2640gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 2700tcagcccgac cactgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 2760cgactttacg ccactggcag cagccattgg taactgaaaa gtggatttag atacgcagaa 2820ctcttgaagt tgaagcctta tcgcggctac actgaaagga cagcatttgg tatctgtgct 2880ccacttaagc cagctaccac aggttagaaa gcctgagaaa cttctaacct tcgaaagaac 2940ccacgcctga gaacgtgggt tttttcgttt acaggcagca gattacgcgc agaaaaaaag 3000gatctcaaga agatcctttg atcttttcta ctgaattgcg ctcccgatca gttcagcaga 3060agattatgat ggggttctat gggtattgct gcggtaacac ccatgttact tgaggttgta 3120tgtagtctgt gtagaattat acacataagg cttaaactgc tctttttttt caatatgcaa 3180ttggaagttc attgactaca taaatagatt attccaaata atttatttat gtaagaacag 3240gatgggaggg ggaatgatct caaagttatt ttgcttggct ctcatatttt tatcatcaag 3300tggccttgca gaaaaaaaca catatacagc aaaagacatc ttgcaaaacc tagaattaaa 3360tacctttggc aattcattgt ctcatggcat ctatgggaaa cagacaacct tcaagcaaac 3420cgagtttaca aatattaaaa gcaacaccaa aaaacacatt gcacttatca ataaagacaa 3480ctcatggatg atatcattaa aaatactagg aattaagaga gatgagtata ctgtctgttt 3540tgaagatttc tctctaataa gaccgccaac atatgtagcc atacatcctc tacttataaa 3600aaaagtaaaa tctggaaact ttatagtagt gaaagaaata aagaaatcta tccctggttg 3660cactgtatat tatcattaat agcaagcccc tcattattat gaggggctca tggttatttt 3720aacaatccac tatcgatatc tttttgcacc agagcgccct ctcgtttacg tctgtcagac 3780attccatcaa caatattatt aaaagcattt acaaggccat tccagtcttt tgcgataact 3840ttattccata ctgtgggagc agttctggat aacttaaacc ctttttgata tccaatagac 3900accagtgctg tacgggttct caacggtaaa tcgctgaacc gaagaccgat attagcgtca 3960ttgaaaagac cttcaatctt atgtgagaat ttatcaatat aaatattaga taagagatga 4020gcttcattat cagaaagcgt cagaggtgct gttctcactt tatcataagc ctccttccct 4080cgaagcatat aatacccatc aagtctatct gcaatatact gagggacacc gtcattcaat 4140aaatcctgtt tgcttcgctg accaaggtca accccggaac cgaatgtaac accggtactg 4200ttaaaataat cgctactagg attagacgga aaatgacttg tcggattaaa cccttcaaaa 4260ccattactgg agaaaatatc gtggtcaaca atatttaccg aacgacgtaa aaattccttc 4320agttgactaa tattgtcaaa gttaatgaca gtgttgtccg ctaggacgat gcgatttcgg 4380ttattattca gaatgtcttc gttctctttc twatcgagat gttcaataga ttcggcaatc 4440gttccctcaa gaaccatgac acggtagact ttcacaccgt ctttttcctg acctgtttca 4500acagttattt tctgttcgta agacacggtc ccttcagttt ttgaaatttt actttcctgg 4560cggatcttat ttgaatattc actgtctttc tccatctccg tatcaatcgg aaaccccata 4620atgtacatca gtttaaaatt actccggcca ggcagatcca cataatgtgg taatgcaatt 4680gtaatcgaat tagcttcaaa atttggtctg taactgctta atgtacttcc ggaaaagaga 4740aaagccggaa caccacctga accattcact accattgtat ctgacataaa aattcctctt 4800taacacataa aaaaacaata agttaaaaaa aaatactgta cataaaacca ctgtttttat 4860gtacagtaat aaaattacgc cgctttattt tctctgtcaa taatatgaaa tttcattttt 4920gtgatctgaa tcactcttat aaaaatcagg aagggaagat tcgcagcaga aaaacagcac 4980cgggtaacat cagaaaaaaa cagaaaggag ataacgtgag caaaacaaaa tctggtcgcc 5040accgactgag caaaacagac aaacgcctgc tggctgcact tgtcgttgcc ggatacgaag 5100aacggacagc ccgtgacctc atccagaaac acgtttacac actgacacag gccgacctgc 5160gccatctggt cagtgaaatc agtaacggtg tgggacagtc acaggcctac gatgcgattt 5220accaggcgag acgcattcgt ctcgcccgta aatacctgag cggaaaaaaa ccggaagggg 5280tggaaccccg ggaagggcag gaacgggaag atttaccata actcccgtta tcagtaccat 5340cggctcaacg ctcgttgtcg gatctgaaaa attcgctcaa aagatcatat ttccctggat 5400attttccacc gtttcttatg tgagcaaagt cacataattc tgtcagacga cgagaaaacg 5460gatatcgatt attgtttaat atttttacat tattaaaaat gaaattagat aatcagatac 5520aaataatatg ttttcgttca tgcagagaga ttaagggtgt ctaatgaaga aaagttctat 5580tgtggcaacc attataacta ttctgtccgg gagtgctaat gcagcatcat ctcagttaat 5640accaaatata tcccctgaca gctttacagt tgcagcctcc accgggatgc tgagtggaaa 5700gtctcatgaa atgctttatg acgcagaaac aggaagaaag atcagccagt tagactggaa 5760gatcaaaaat gtcgctatcc tgaaaggtga tatatcctgg gatccatact catttctgac 5820cctgaatgcc agggggtgga cgtctctggc ttccgggtca ggtaatatgg atgactacga 5880ctggatgaat gaaaatcaat ctgagtggac agatcactca tctcatcctg ctacaaatgt 5940taatcatgcc aatgaatatg acctcaatgt gaaaggctgg ttactccagg atgagaatta 6000taaagcaggt ataacagcag gatatcagga aacacgtttc agttggacag ctacaggtgg 6060ttcatatagt tataataatg gagcttatac cggaaacttc ccgaaaggag tgcgggtaat 6120aggttataac cagcgctttt ctatgccata tattggactt gcaggccagt atcgcattaa 6180tgattttgag ttaaatgcat tatttaaatt cagcgactgg gttcgggcac atgataatga 6240tgagcactat atgagagatc ttactttccg tgagaagaca tccggctcac gttattatgg 6300taccgtaatt aacgctggat attatgtcac acctaatgcc aaagtctttg cggaatttac 6360atacagtaaa tatgatgagg gcaaaggagg tactcagacc attgataaga atagtggaga 6420ttctgtctct attggcggag atgctgccgg tatttccaat aaaaattata ctgtgacggc 6480gggtctgcaa tatcgcttct gaaaaataca gatcatatct ctcttttcat cctcccctag 6540cggggaggat gtctgtggaa aggaggttgg tgtttgacca accttcagat gtgtgaaaaa 6600tcaccttttt caccataatg acggggcgct cattctgttg ttttgccttg acattctcca 6660cgtctttcag ggcatggaga aggtcaaatt agacatggaa cgctactctc cttcctgtag 6720gaagctcaac atccaagctt aatttgcctc ccattgcttc aacgtaacgc tttaacgtcg 6780ccagctttaa atcatttccg cgctgctcca gctttgttac tgctggctgg cttataccca 6840tcgcctcagc aacttgtttt tgtgataact ggagttcttc acgcatcatc tgcaagccga 6900cctcaagaat catctcatct gccatttctt taattcgtgt ctggctttca ggtgaacgac 6960tggcaatcac ctcatctaat gttctcatta cttgctctcc agtgtgttca gatgtgctgt 7020aaattcatcc tcagctatac gcaccagttt ttcataaaac cgcttatcat tacttttatc 7080tcctgcacaa agaacgatag cccgacgaat cggatcgaac gcataaaagg ctcttatcgg 7140acggccagaa aactgaacgc gaagctcttt catatttttg taccgagaac ctttcacggt 7200atcggcatat ggcctgggta actcaggtcc gtaaacctgt agctttttca aatcagccaa 7260aaccttttcc tgaagagcgt cttcttgctc atttagccag tcgtcaaatc gctggctaaa 7320aagtaccatc cacatgctca accctataac ctgtagctta ccccactaac aatataacct 7380acgagttata ttttcaagaa aagctggcta tttaacataa cggcaatttg tacgcaccac 7440tgaaatgcgt tcagcgcgat cacggcaaca gacaggcaaa aatagcaaca aacctcccga 7500aaaaccgccg cgatcgcgcc tgataaattt taaccttatg catatctatg cagccaggcg 7560aatcacgaac gaattgcctg cctgatgtaa ctgaaacggg tgttttttcc tgatttggtg 7620ggcgtggaag acggaacatg aacgggaaaa cagaattcat gccagatgag cgcgatctgg 7680caattaaggc aaaacacagc aacaaagaca cgccagaatc gcgcccggat atgttttaac 7740gcgattttca gactcagaca aattcagcag aatgctactc cattcaccgg gctgatggtg 7800aatacatgcg tatccaggat gagtacattt ctggctctgc cacagctctg tctgttggca 7860gctttcgcct gtccggaaac ctgcttaaaa cgctcccgaa aggcctctga accagaaagc 7920aacaaaacac aggccattaa gtaaatcgcg ttaaaacacg tctgatggat tgctgcaaaa 7980aaaagtccct aatggagcag ggactgttaa acccagtgaa tagcgtctaa attaaagtaa 8040gaatacgacc aggtactctt cagaaaagag attaatccac cgcacagaat aatcaacagt 8100aaaaacaaac aaccctgatt ttttattttt ctttttttcg ataaaaacaa aattaaagaa 8160ataattaatc agaacattcc ttaacttcag ggcattgcct gtgttccatt ttgtgattag 8220tctgaaactt ccgaaggtgg ataacacccg gtattttttt gctcacataa agcccctcct 8280tcaggcagag gggctttttc tttgccacca cataaaaaag gccctcacag gaggtgttct 8340gtgagggcgt atgataagga ctgaatcgat ggttaatatg tctagtcctg acttttgcat 8400ctccgaatat aaaaccctgt ttaacggcat gcaaaaccaa aaaataaaaa tgtgacatcg 8460caatgccaga taatattgac gcatgaggga atgcgtaccc cgacccctgt gtaacgaacg 8520gtgcaatagt gatccacacc caacgcctga aatcagatcc agggggtaat ctgctctcct 8580gattcaggag agtttatggt cacttttgag acagttatgg aaattaaaat cctgcacaag 8640cagggaatga gtagccgggc gattgccaga gaactgggga tctcccgcaa taccgttaaa 8700cgttatttgc aggcaaaatc tgagccgcca aaatatacgc cgcgacctgc tgttgcttca 8760ctcctggatg aataccggga ttatattcgt caacgcatcg ccgatgctca tccttacaaa 8820atcccggcaa cggtaatcgc tcgcgagatc agagaccagg gatatcgtgg cggaatgacc 8880attctcaggg cattcattcg ttctctctcg gttcctcagg agcaggagcc tgccgttcgg 8940ttcgaaactg aacccggacg acagatgcag gttgactggg gcactatgcg taatggtcgc 9000tcaccgcttc acgtgttcgt tgctgttctc ggatacagcc gaatgctgta catcgaattc 9060actgacaata tgcgttatga cacgctggag acctgccatc gtaatgcgtt ccgcttcttt 9120ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac 9180gcatatcaga ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg 9240ggcttctctc cccgactgtg tcgccccttc agggcacaga ctaaaggtaa ggtggaacgg 9300atggtgcagt acacccgtaa cagtttttac atcccactaa tgactcgcct gcgcccgatg 9360gggatcactg tcgatgttga aacagccaac cgccacggtc tgcgctggct gcacgatgtc 9420gctaaccaac gaaagcatga aacaatccag gcccgtccct gcgatcgctg gctcgaagag 9480cagcagtcca tgctggcact gcctccggag aaaaaagagt atgacgtgca tcttgatgaa 9540aatctggtga acttcgacaa acaccccctg catcatccac tctccatcta cgactcattc 9600tgcagaggag tggcgtgatg atggaactgc aacatcaacg actgatggcg ctcgccgggc 9660agttgcaact ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc 9720aggaatggag ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc 9780atcaacgtaa acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg 9840aagagtatga cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct 9900cactcagctt catagaacgt aatgaaaata tcgtattcac tggggccatt caggtgtggg 9960

Claims

1. A recombinant DNA construction comprising an open reading frame placed under the control of a non-native promoter, the open reading frame being SEQ ID NO:3, base pairs 2389 to 2826, as found in Yersinia pestis plasmid pPCP 1.

2. A host transformed with the DNA construction of claim 1.