Claims
- 1. An isolated DNA construct comprising a polynucleotide sequence encoding a glycoprotein V polypeptide.
- 2. The DNA construct of claim 1, wherein the polynucleotide sequence encodes a polypeptide having an amino acid sequence as shown in FIG. 5B (SEQ.ID.NO. 2).
- 3. The DNA construct of claim 1, wherein the polynucleotide sequence has a sequence as shown in FIG. 5A (SEQ.ID.NO. 1).
- 4. The DNA construct of claim 1, wherein the polynucleotide sequence lacks introns.
- 5. The DNA construct of claim 1, wherein the polynucleotide sequence encodes a full length glycoprotein V protein.
- 6. The DNA construct of claim 1, further comprising a heterologous promoter operably linked to the polynucleotide sequence.
- 7. The DNA construct of claim 6, wherein the promoter directs expression of the polynucleotide sequence in a eukaryote.
- 8. The DNA construct of claim 6, wherein the promoter directs expression of the polynucleotide sequence in a prokaryote.
- 9. A composition comprising a cell containing a DNA construct of claim 1.
- 10. The composition of claim 9, wherein the cell is a eukaryote.
- 11. The composition of claim 9, wherein the cell is a prokaryote.
- 12. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and the GPV polypeptide.
- 13. The composition of claim 12, wherein the GPV polypeptide has a sequence as shown in FIG. 5B (SEQ.ID.NO. 2).
- 14. An isolated, purified GPV polypeptide having a sequence as shown in FIG. 5B (SEQ.ID.NO. 2).
- 15. An antibody capable of reacting with the polypeptide of claim 14.
- 16. A method for inducing a degree of thrombocytopenia in a patient comprising the administration of the antibody of claim 15.
- 17. The use of an isolated GPV promoter to direct expression of a polypeptide.
- 18. A method for the detection of cells of megakaryoblastic lineage, comprising evaluating a sample for the presence or absence of a polynucleotide sequence as shown in FIG. 5A (SEQ.ID.NO. 1).
- 19. A method for detecting thrombus formation in a subject, comprising the measurement of circulating GPVf1.
- 20. An isolated nucleic acid capable of hybridizing to a nucleic acid having a sequence of FIG. 5A (SEQ.ID.NO. 1).
Parent Case Info
[0001] This application is a Continuation-in-Part of U.S. application Ser. No. 08/089,455 filed Jul. 9, 1993.
Divisions (1)
|
Number |
Date |
Country |
Parent |
08195006 |
Feb 1994 |
US |
Child |
08884571 |
Jun 1997 |
US |
Continuations (2)
|
Number |
Date |
Country |
Parent |
09560814 |
Apr 2000 |
US |
Child |
10212499 |
Aug 2002 |
US |
Parent |
08884571 |
Jun 1997 |
US |
Child |
09560814 |
Apr 2000 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
08089455 |
Jul 1993 |
US |
Child |
08195006 |
Feb 1994 |
US |