Claims
- 1. A method for preparing a polypeptide having a molecular weight of approximately 2,000 and having an affinity for lipopolysaccharides, which comprising hypotonic extraction of horseshoe crab hemocyte to obtain a hypotonic extraction residue; extracting said residue under acidic conditions sufficient to have the hypotonic extraction residue release said polypeptide to obtain a hypotonic extraction residue extract; and purifying said polypeptide in said extract.
- 2. The method for preparing the polypeptide according to claim 1, wherein the horseshoe crab is at least one of Tachypleus tridentatus, Tachypleus gigas and Limulus polyphemus.
Priority Claims (1)
Number |
Date |
Country |
Kind |
62-206258 |
Aug 1987 |
JPX |
|
Parent Case Info
This is a continuation, of Ser. No. 665,819 filed Mar. 7, 1991, now abandoned, which in turn is a division of U.S. patent application Ser. No. 348,487 filed Apr. 19, 1989, now U.S. Pat. No. 5,068,314.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5068314 |
Nakamura et al. |
Nov 1991 |
|
Non-Patent Literature Citations (6)
Entry |
Morita, et al., J. Biochem., 97, 1611-1620 (1985). |
Annex I--J. Biochem., 97, 1611-1620 (1985). |
Annex II--vol. 261, No. 16, pp. 7357-7365, Jun. 5 issue. |
Annex III--Eur. J. Biochem., 154, 511-521 (1986). |
Annex IV--vol. 263, No. 32, Nov. 15 issue, pp. 16709-16713. |
Annex V--vol. 252, No. 1, 2, 121-124, Jul. 1989. |
Divisions (1)
|
Number |
Date |
Country |
Parent |
348487 |
Apr 1989 |
|
Continuations (1)
|
Number |
Date |
Country |
Parent |
665819 |
Mar 1991 |
|