Claims
- 1. A probe for analyzing protein-protein interaction between two proteins, wherein protein splicing is induced by protein-protein interaction, thereby regenerating a physiochemically dr biochemically detectable protein.
- 2. The probe for protein-protein interaction analysis of claim 1, consisting of two probes that are: probe a which comprises the N-terminal polypeptide of an intein and the N-terminal polypeptide of a labeled protein; and probe b which comprise the C-terminal polypeptide of the intein and the C-terminal polypeptide of the labeled protein.
- 3. The probe for protein-protein interaction analysis of claim 2, wherein the C-terminal of probe a and the N-terminal of probe b each contain a linker sequence.
- 4. The probe for protein-protein interaction analysis of claim 2 or 3, wherein the intein is an endonuclease derived from yeast VMA.
- 5. The probe for protein-protein interaction analysis of claim 2 or 3, wherein the intein is DnaE derived from cyanobacterium.
- 6. The probe for protein-protein interaction analysis of claims 2 to 5, wherein the labeled protein is a fluorescent protein.
- 7. The probe for protein-protein interaction analysis of claim 6, wherein the fluorescent protein is a green fluorescent protein.
- 8. The probe for protein-protein interaction analysis of claims 2 to 5, wherein the labeled protein is a luminescent enzyme.
- 9. The probe for protein-protein interaction analysis of claim 8, wherein the luminescent enzyme is a luciferase.
- 10. A method for analyzing protein-protein interaction, comprising:
making a protein linked with probe a as described in claims 2 to 9 and a protein linked with probe b as described in claims 2 to 9 coexist in a system; and detecting the signal emitted by the labeled protein.
- 11. The method of protein-protein interaction analysis of claim 10, wherein a polynucleotide that expresses the probe of any one of claims 1 to 9 is introduced into a eucaryotic cell, thereby making the probe a-linked protein and the probe b-linked protein coexist in the cell.
Priority Claims (1)
Number |
Date |
Country |
Kind |
2000-224939 |
Jul 2000 |
JP |
|
Parent Case Info
[0001] This application is a 371 of PCT/JP00/09348 filed Dec. 27, 2000.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/JP00/09348 |
12/27/2000 |
WO |
|