Claims
- 1. A process for increasing the production of .beta.-galactosidase by a culture of E. coli comprising the steps of:
- a) adding a culture of E. coli having a pTREZ1 hybrid plasmid containing a tryptophan promoter and a .beta.-galactosidase gene to a first medium for cultivation, wherein said first medium contains an initial growth supporting level of glucose;
- b) adding oxygen to said first medium to promote the multiplication of the E. coli, wherein said oxygen is added to the first cultivation medium at a constant flow rate;
- c) monitoring the dissolved oxygen concentration of the first cultivation medium; and,
- d) adding simultaneously 3-.beta.-indolylacrylic acid and a second medium containing casamino acid to the first cultivation medium at a time when the dissolved oxygen concentration of said first cultivation medium changes from a decrease to an increase.
- 2. A process for increasing the production of .beta.-galactosidase by a culture of E. coli comprising the steps of:
- a) adding a culture of E. coli having a pTREZ1 hybrid plasmid containing a tryptophan promoter and a .beta.-galactosidase gene to a first medium for cultivation, wherein said first medium contains an initial growth supporting level of glucose;
- b) adding tryptophan to the first cultivation medium, wherein said tryptophan suppresses the production of .beta.-galactosidase by the E. coli;
- c) adding oxygen to said first medium to promote the multiplication of the E. coli, wherein said oxygen is added to the first cultivation medium at a constant flow rate;
- d) monitoring the dissolved oxygen concentration of the first cultivation medium; and,
- e) adding simultaneously 3-.beta.-indolylacrylic acid and a second medium containing casamino acid to the first cultivation medium at a time when the dissolved oxygen concentration of said first cultivation medium changes from a decrease to an increase.
- 3. The process of claim 1, wherein said E. coli is selected from the group consisting of E. coli M182, E. coli HB101, E. coli C600, E. coli X1776, and E. coli DH1.
- 4. The process of claim 2, wherein said E. coli is selected from the group consisting of E. coli M182, E. coli HB101, E. coli C600, E. coli X1776, and E. coli DH1.
- 5. A process for increasing the production of .beta.-galactosidase by a culture of E. coli comprising the steps of:
- a) adding E. coli M182 having a pTREZ1 hybrid plasmid containing a tryptophan promoter and a .beta.-galactosidase gene to a first medium for cultivation, wherein said first medium contains an initial growth supporting level of glucose;
- b) adding oxygen to said first medium to promote the multiplication of the E. coli, wherein said oxygen is added to the first cultivation medium at a constant flow rate;
- c) monitoring the dissolved oxygen concentration of the first cultivation medium; and,
- d) adding simultaneously 3-.beta.-indolylacrylic acid and a second medium containing casamino acid to the first cultivation medium at a time when the dissolved oxygen concentration of said first cultivation medium changes from a decrease to an increase.
- 6. A process for increasing the production of .beta.-galactosidase by a culture of E. coli comprising the steps of:
- a) adding a culture of E. coli M182 having a pTREZ1 hybrid plasmid containing a tryptophan promoter and a .beta.-galactosidase gene to a first medium for cultivation, wherein said first medium contains an initial growth supporting level of glucose;
- b) adding tryptophan to the first cultivation medium, wherein said tryptophan suppresses the production of .beta.-galactosidase by the E. coli;
- c) adding oxygen to said first medium to promote the multiplication of the E. coli, wherein said oxygen is added to the first cultivation medium at a constant flow rate;
- d) monitoring the dissolved oxygen concentration of the first cultivation medium; and,
- e) adding simultaneously 3-.beta.-indolylacrylic acid and a second medium containing casamino acid to the first cultivation medium at a time when the dissolved oxygen concentration of said first cultivation medium changes from a decrease to an increase.
Priority Claims (2)
Number |
Date |
Country |
Kind |
59-127472 |
Jun 1984 |
JPX |
|
59-191603 |
Sep 1984 |
JPX |
|
Parent Case Info
This is a continuation of application Ser. No. 746,795 filed on Jun. 20, 1985, now abandoned.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
3912585 |
Iijima et al. |
Oct 1975 |
|
4551433 |
DeBoer |
Nov 1985 |
|
4891310 |
Shimizu et al. |
Jan 1990 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
0036776 |
Sep 1981 |
EPX |
Continuations (1)
|
Number |
Date |
Country |
Parent |
746795 |
Jun 1985 |
|