Claims
- 1. A method for identifying a perturbagen that inhibits viral growth, comprising the steps of:
(a) introducing a perturbagen encoding nucleic acid into a cell; (b) exposing said perturbagen-bearing cell to a virus; and (c) selecting for growth-proficient cells.
- 2. The method of claim 1, wherein said step of selecting for growth-proficient cells comprises detecting cells that are not productively infected with said virus.
- 3. The method of claim 2, wherein said step of detection comprises detection of non-fluorescent cells.
- 4. The method of claim 1, wherein said step of selecting for growth-proficient cells comprises a stringent selection for growth.
- 5. The method of claim 1, wherein said perturbagen is in a scaffold.
- 6. The method of claim 5, wherein said scaffold is non-fluorescing GFP.
- 7. The method of claim 1 wherein said virus is selected from the group consisting of rhinovirus, reovirus, influenza virus, adenovirus, human immunodeficiency virus, human papilloma virus, hepatitis virus and herpes virus.
- 8. The method of claim 7 wherein said virus is human immunodeficiency virus.
- 9. A method for identifying a cell proliferation gene involved in viral growth, comprising the steps of:
(a) identifying within a plurality of virally infected cells a cell that continues to proliferate; and (b) identifying within said cell that continues to proliferate a corresponding cell proliferation gene.
- 10. The method of claim 9, wherein said cell that continues to proliferate was transduced with a perturbagen prior to viral infection.
- 11. A method for identifying a cellular target involved in viral growth within a cell, comprising the steps of:
(l) identifying within a plurality of virally infected cells a cell that continues to proliferate; and (m) identifying within said cell that continues to proliferate a corresponding cellular target.
- 12. The method of claim 11, wherein said cell that continues to proliferate was transduced with a perturbagen prior to being virally infected.
- 13. The method of claim 12, wherein said step of identifying comprises a protein/protein interaction assay.
RELATED APPLICATIONS
[0001] This application is a continuation-in-part of U.S. application Ser. No. 08/699,266, and is a continuation-in-part of [attorney docket no. VEN-2 CIP, US continuation of PCT/US97/14514, filed Feb. 18, 1999], the entire disclosures of which are incorporated by reference herein in their entirety. This application also is a continuation-in-part of U.S. application Ser. Nos. 08/812,994 and 08/965,477, the entire disclosures of which are incorporated by reference herein in their entirety.
Continuations (1)
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Date |
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Parent |
09259155 |
Feb 1999 |
US |
Child |
09929663 |
Aug 2001 |
US |
Continuation in Parts (4)
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Number |
Date |
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Parent |
08699266 |
Aug 1996 |
US |
Child |
09259155 |
Feb 1999 |
US |
Parent |
PCT/US97/14514 |
Feb 1999 |
US |
Child |
09259155 |
Feb 1999 |
US |
Parent |
08812994 |
Mar 1997 |
US |
Child |
09929663 |
Aug 2001 |
US |
Parent |
08965477 |
Nov 1997 |
US |
Child |
09929663 |
Aug 2001 |
US |