Claims
- 1. An Escherichia coli or Erwinia chrysanthemi strain having a chromosome comprising multiple stably integrated copies of a recombinant transposon, wherein said transposon is incapable of expressing a functional transposase and comprises, inserted therein, a chosen DNA molecule.
- 2. The bacterial strain of claim 1 wherein said recombinant transposon is from a Mu phage.
- 3. The bacterial strain of claim 2, wherein said Mu phage is MudI or MudII.
- 4. The bacterial strain of claim 1 wherein said recombinant transposon is Tn3, Tn5, Tn7, Tn10, Tn903, TnHoHo, IS 1, IS 10, IS 50 or a phage having the capacity to transpose.
- 5. The bacterial strain of claim 1 wherein said chosen DNA molecule comprises a chosen gene.
- 6. The bacterial strain of claim 5, wherein said chosen gene is a gene encoding a protein of the biosynthetic pathway of an amino acid.
- 7. The bacterial strain of claim 6, wherein said amino acid is L-threonine.
- 8. The bacterial strain of claim 6, wherein, relative to its parental strain, said strain is an over-producer of said amino acid.
- 9. The bacterial strain of claim 8, wherein said amino acid is L-threonine.
- 10. The bacterial strain of claim 1, wherein said chosen DNA molecule comprises an operon encoding a protein of the biosynthetic pathway of an amino acid.
- 11. The bacterial strain of claim 10, wherein said operon encodes a protein of the biosynthetic pathway of L-threonine.
- 12. An Escherichia coli or Erwinia chrysanthemi strain having an episome comprising multiple stably integrated copies of a recombinant transposon, wherein said transposon is incapable of expressing a functional transposase and comprises, inserted therein, a chosen DNA molecule.
- 13. The bacterial strain of claim 12, wherein said recombinant transposon is from a Mu phage.
- 14. The bacterial strain of claim 13 wherein said Mu phage is MudI or MudII.
- 15. The bacterial strain of claim 12, wherein said recombinant transposon is Tn3, Tn5, Tn7, TnlO, Tn903, TnHoHo, IS 1, IS 10, IS 50 or a phage having the capacity to transpose.
- 16. The bacterial strain of claim 12, wherein said chosen DNA molecule comprises a chosen gene.
- 17. The bacterial strain of claim 12, wherein said chosen gene is a gene encoding a protein of the biosynthetic pathway of an amino acid.
- 18. The bacterial strain of claim 17, wherein said amino acid is L-threonine.
- 19. The bacterial strain of claim 17, wherein relative to its parental strain, said strain is an over-producer of said amino acid.
- 20. The bacterial strain of claim 19, wherein said amino acid is L-threonine.
- 21. The bacterial strain of claim 12, wherein said chosen DNA molecule comprises an operon encoding a protein of the biosynthetic pathway of an amino acid.
- 22. The bacterial strain of claim 21, wherein said operon encodes a protein of the biosynthetic pathway of L-threonine.
- 23. A method for producing an amino acid by fermentation comprising culturing a Escherichia coli or Erwinia chrysanthemi strain having a chromosome comprising multiple stably integrated copies of a recombinant transposon, wherein said transposon is incapable of expressing a functional transposase and comprises, inserted therein, a chosen DNA molecule comprising a gene encoding a protein of the biosynthetic pathway of said amino acid, under conditions in which said gene is expressed, whereby said amino acid is produced.
- 24. The method of claim 23, wherein said recombinant transposon is from a Mu phage.
- 25. The method of claim 24, wherein said Mu phage is MudI or MudII.
- 26. The method of claim 23, wherein said recombinant transposon is Tn3, Tn5, Tn7, Tn10, Tn903, TnHoHo, IS 1, IS 10, IS 50 or a phage having the capacity to transpose.
- 27. The method of claim 23 wherein said chosen DNA molecule comprises a chosen gene.
- 28. The method of claim 27, wherein said chosen gene is a gene encoding a protein of the biosynthetic pathway of an amino acid.
- 29. The method of claim 28, wherein said amino acid is L-threonine.
- 30. The method of claim 28, wherein, relative to its parental strain, said strain is an over-producer of said amino acid.
- 31. The method of claim 30, wherein said amino acid is L-threonine.
- 32. The method of claim 23, wherein said chosen DNA molecule comprises an operon encoding a protein of the biosynthetic pathway of an amino acid.
- 33. The method of claim 32, wherein said operon encodes a protein of the biosynthetic pathway of L-threonine.
- 34. A method for producing an amino acid by fermentation comprising culturing a Escherichia coli or Erwinia chrysanthemi strain having an episome comprising multiple stably integrated copies of a recombinant transposon, wherein said transposon is incapable of expressing a functional transposase and comprises, inserted therein, a chosen DNA molecule comprising a gene encoding a protein of the biosynthetic pathway of said amino acid, under conditions in which said gene is expressed whereby said amino acid is produced.
- 35. The method of claim 34, wherein said recombinant transposon is from a Mu phage.
- 36. The method of claim 35, wherein said Mu phage is MudI or MudII.
- 37. The method of claim 34, wherein said recombinant transposon is Tn3, Tn5, Tn7, Tn10, Tn903, TnHoHo, IS 1, IS 10, IS 50 or a phage having the capacity to transpose.
- 38. The method of claim 34, wherein said chosen DNA molecule comprises a chosen gene.
- 39. The method of claim 38, wherein said chosen gene is a gene encoding a protein of the biosynthetic pathway of an amino acid.
- 40. The method of claim 39, wherein said amino acid is L-threonine.
- 41. The method of claim 39, wherein relative to its parental strain, said strain is an over-producer of said amino acid.
- 42. The method of claim 41, wherein said amino acid is L-threonine.
- 43. The method of claim 34, wherein said chosen DNA molecule comprises an operon encoding a protein of the biosynthetic pathway of an amino acid.
- 44. The method of claim 43, wherein said operon encodes a protein of the biosynthetic pathway of L-threonine.
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a Continuation of U.S. application Ser. No. 08/436,113, filed May 8, 1995 now abandoned, which is a continuation of U.S. application Ser. No. 08/341,460, filed Nov. 17, 1994 now U.S. Pat. No. 5,595,889, which is a continuation of U.S. application Ser. No. 07/859,610, filed Mar. 23, 1992 now abandoned, which is a continuation of U.S. application Ser. No. 07/313,625, filed Feb. 21, 1989 now abandoned.
Non-Patent Literature Citations (4)
Entry |
Neidhardt, F (1987), E. coli and S. typhimurium, vol. 2-pp. 1071-1109. |
Gramajo, H. et al (1988). Gene 65, 305-314. |
Parsot, C. (1986). The Embo Journal 5, 3013-19. |
Maniatis, T. et al (1982) In Molecular Cloning A Laboratory Manual; Cold Spring Harbor Press, NJ. pp. 403-434. |
Continuations (4)
|
Number |
Date |
Country |
Parent |
08/436113 |
May 1995 |
US |
Child |
08/941122 |
|
US |
Parent |
08/341460 |
Nov 1994 |
US |
Child |
08/436113 |
|
US |
Parent |
07/859610 |
Mar 1992 |
US |
Child |
08/341460 |
|
US |
Parent |
07/313625 |
Feb 1989 |
US |
Child |
07/859610 |
|
US |