Claims
- 1. A process for integrating nucleic acid into the genome of mammalian cells comprising, forming an integrator complex between the nucleic acid containing a transposon and a transposase specific for the transposon; and, delivering the integrator complex to a mammalian cell.
- 2. The process of claim 1 wherein the nucleic acid encodes an expressible gene.
- 3. The process of claim 2 wherein the expressible gene encodes a protein.
- 4. The process of claim 2 wherein the expressible gene encodes a functional RNA.
- 5. The process of claim 4 wherein the functional RNA is a siRNA.
- 6. A composition for integrating nucleic acids into a mammalian genome, comprising, a Tn5 integrator complex consisting of Tn5 transposase and a mammalian nucleic acid sequence.
- 7. The process of claim 1 wherein the integrator complex is formed prior to delivery.
- 8. The process of claim 1 wherein the transposase consists of a hyperactive transposase.
- 9. The process of claim 8 where in the hyperactive transposase is the EK54, MA56, LP372 transposase.
- 10. The process of claim 1 wherein the transposase contains a nuclear localization signal.
- 11. A process for integrating a nucleic acid into amammalianchromosome, comprising: forming an integrator complex in solution; associating the complex with a transfection reagent; delivering the complex to a mammalian cell wherein the nucleic acid is integrated into the mammalian chromosome.
- 12. The process of claim 11 wherein the transposon contains elements selected from the group consisting of: outer elements, inner elements, and mosaic elements.
Parent Case Info
[0001] This application claims priority benefit of U.S. Provisional Applications Serial No. 60/329474 filed Oct. 15, 2001 and Serial No. 60/344,865 filed Nov. 8, 2001.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60329474 |
Oct 2001 |
US |
|
60344865 |
Nov 2001 |
US |