Prostaglandin transporter inhibitors

BACKGROUND OF THE INVENTION

(1) Field of the Invention

The present invention generally relates to prostaglandin transport. More specifically, the invention is directed to compounds that inhibit prostaglandin transport and subsequent COX-2 induction, and methods relating thereto.

(2) Description of the Related Art

Prostaglandins (PGs) are synthesized from arachidonic acid by cyclooxygenases (COX1 and COX2) and corresponding synthases (Helliwell et al., 2004). PGs play an important role in physiology and clinical settings. Their biological effects include triggering inflammation, fever and pain (Blatteis and Sehic, 1997; Bley et al., 1998; Vanegas and Schaible, 2001; Samad et al., 2002); induction of labor (Ulmann et al., 1992); modulation of renal hemodynamics and of water and solute reabsorption (Epstein, 1986; Wang et al., 1998; Yokoyama et al., 2002); and arterial vasodilalation (Clyman et al., 1978; Coceani and Olley, 1988; Smith et al., 1994). PG analogues, such as latanoprost and unoprostone, have been used to treat glaucoma (Stjernschantz, 1995; Alm, 1998; Stisanna et al., 2002; Stjernschantz, 2004). At the cellular level, PGs are involved in several major signaling pathways, including the MAP kinase and protein kinase A pathways by upregulation of cAMP (Naruminya et al., 1999; Bos et al., 2004).

The magnitude of PG effects depends not only on their production but also their metabolism. The prostaglandin transporter (PGT) (Kanai et al., 1995) removes PGs from the extracellular compartment and thereby terminates their interactions with receptors on cell membranes. PGT delivers PGs to cytoplasmic 15-OH PG dehydrogenase (Schuster, 2002; Nomura et al., 2004), resulting in oxidation and inactivation.

Because PGT is highly expressed in the tissues and organs where PGs are synthesized (Bao et al., 2002), and because PGT regulates a broad and complex PG signaling system, an inhibitor of PGT would be important for manipulating signaling. Known PGT blockers include inhibitors of the organic anion transporters (OATs), such as bromcresol green and bromosulfophthalein, and some COX2 inhibitors, such as indomethacin and ibuprofen (Bito and Salvador, 1976; Kanai et al., 1995). One of the main problems with these inhibitors is that they are not specific for PGT (Jacquemin et al., 1994; Sweet et al., 1997).

It would thus be desirable to identify specific PGT inhibitors. The present invention addresses that need.

SUMMARY OF THE INVENTION

Accordingly, the inventors have identified compounds that inhibit mammalian prostaglandin transporter (PGT) activity. These compounds are useful, e.g., in the treatment of disorders that are at least partially mediated by excessive PGT activity.

Thus, the present invention is directed to methods of inhibiting prostaglandin transporter (PGT) activity in mammals. The methods comprise administering a compound to the mammal effective to inhibit PGT activity. In these methods, the compound has Formula I:

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or a pharmaceutically acceptable salt, ester, or tautomer thereof, wherein R is a C₁-C₁₅straight or branched alkyl, a substituted alkyl, a cycloalkyl, a carboxyalkyl, a substituted cycloalkyl, a C₁-C₁₅straight or branched alkenyl, a substituted alkenyl, a cycloalkenyl, a substituted cycloalkenyl, a C₁-C₁₅straight or branched alkinyl, a substituted alkinyl, a cycloalkinyl, a substituted cycloalkinyl, a C₁-C₁₀straight or branched ether, a substituted ether, a cycloether, an ester, an amide, an acetyl, an aminal, an anhydride, an aryl, a substituted aryl, a heteroaryl, a substituted heteroaryl, a carboxyaryl, a heterocyclic group, a substituted heterocyclic group, a fused cycloalkyl, a substituted fused cycloalkyl, a fused heterocyclic group, a substituted fused heterocyclic group, a fused aryl, a substituted fused aryl, a fused heteroaryl, a substituted fused heteroaryl ring, or any combination thereof, optionally further comprising a hydroxy, an alkoxy, an aryloxy, an oxo, an ester, an ether, an amine, an azo, an azido, a nitro, an imine, an isothionate, a carbonyl, a peroxide, a halogen, a formyl, an acyl, a carboxy, an amido, a carbamoyl, a guanidino, a ureido, a amidino, a thiol, a mercapto, a sulfinyl, a sulfonyl and/or a sulfonamide.

The invention is also directed to methods of determining whether a test compound is an inhibitor of a prostaglandin transporter. These methods comprise contacting the test compound with the prostaglandin transporter, then determining whether the prostaglandin transporter has less transporter activity when contacted with the test compound than when no so contacted. The test compound in these methods is

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or a pharmaceutically acceptable salt, ester, or tautomer thereof, wherein R1 and R2 are independently a C₁-C₁₅straight or branched alkyl, a substituted alkyl, a cycloalkyl, a carboxyalkyl, a substituted cycloalkyl, a C₁-C₁₅straight or branched alkenyl, a substituted alkenyl, a cycloalkenyl, a substituted cycloalkenyl, a C₁-C₁₅straight or branched alkinyl, a substituted alkinyl, a cycloalkinyl, a substituted cycloalkinyl, a C₁-C₁₀straight or branched ether, a substituted ether, a cycloether, an ester, an amide, an acetyl, an aminal, an anhydride, an aryl, a substituted aryl, a heteroaryl, a substituted heteroaryl, a carboxyaryl, a heterocyclic group, a substituted heterocyclic group, a fused cycloalkyl, a substituted fused cycloalkyl, a fused heterocyclic group, a substituted fused heterocyclic group, a fused aryl, a substituted fused aryl, a fused heteroaryl, a substituted fused heteroaryl ring, or any combination thereof, optionally further comprising a hydroxy, an alkoxy, an aryloxy, an oxo, an ester, an ether, an amine, an azo, an azido, a nitro, an imine, an isothionate, a carbonyl, a peroxide, a halogen, a formyl, an acyl, a carboxy, an amido, a carbamoyl, a guanidino, a ureido, a amidino, a thiol, a mercapto, a sulfinyl, a sulfonyl and/or a sulfonamide.

The invention is further directed to compounds that inhibit prostaglandin transporter activity. These compounds are

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The invention is additionally directed to pharmaceutical compositions comprising any of the above compounds in a pharmaceutically acceptable excipient.

The invention is further directed to methods of inhibiting prostaglandin transporter (PGT) activity in a mammal. The methods comprise administering a compound to the mammal effective to inhibit PGT activity. In these methods, the compound is

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Additionally, the invention is directed to methods of determining whether a test compound is an inhibitor of a prostaglandin transporter. The methods comprise contacting the test compound with the prostaglandin transporter, then determining whether the prostaglandin transporter has less transporter activity when contacted with the test compound than when not so contacted. In these methods, the test compound is

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or a pharmaceutically acceptable salt, ester, or tautomer thereof, wherein R1, R2 and R3 are independently a C₁-C₁₅is straight or branched alkyl, a substituted alkyl, a cycloalkyl, a carboxyalkyl, a substituted cycloalkyl, a C₁-C₁₅straight or branched alkenyl, a substituted alkenyl, a cycloalkenyl, a substituted cycloalkenyl, a C₁-C₁₅straight or branched alkinyl, a substituted alkinyl, a cycloalkinyl, a substituted cycloalkinyl, a C₁-C₁₀straight or branched ether, a substituted ether, a cycloether, an ester, an amide, an acetyl, an aminal, an anhydride, an aryl, a substituted aryl, a heteroaryl, a substituted heteroaryl, a carboxyaryl, a heterocyclic group, a substituted heterocyclic group, a fused cycloalkyl, a substituted fused cycloalkyl, a fused heterocyclic group, a substituted fused heterocyclic group, a fused aryl, a substituted fused aryl, a fused heteroaryl, a substituted fused heteroaryl ring, or any combination thereof, optionally further comprising a hydroxy, an alkoxy, an aryloxy, an oxo, an ester, an ether, an amine, an azo, an azido, a nitro, an imine, an isothionate, a carbonyl, a peroxide, a halogen, a formyl, an acyl, a carboxy, an amido, a carbamoyl, a guanidino, a ureido, a amidino, a thiol, a mercapto, a sulfinyl, a sulfonyl and/or a sulfonamide, and wherein R3 is not

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The invention is also directed to other methods of determining whether a test compound is an inhibitor of a prostaglandin transporter. These methods comprise contacting the test compound with the prostaglandin transporter, then determining whether the prostaglandin transporter has less transporter activity when contacted with the test compound than when no so contacted. Here, the test compound is

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or a pharmaceutically acceptable salt, ester, or tautomer thereof, wherein R1 and R3 are independently a C₁-C₁₅straight or branched alkyl, a substituted alkyl, a cycloalkyl, a carboxyalkyl, a substituted cycloalkyl, a C₁-C₁₅straight or branched alkenyl, a substituted alkenyl, a cycloalkenyl, a substituted cycloalkenyl, a C₁-C₁₅straight or branched alkinyl, a substituted alkinyl, a cycloalkinyl, a substituted cycloalkinyl, a C₁-C₁₀straight or branched ether, a substituted ether, a cycloether, an ester, an amide, an acetyl, an aminal, an anhydride, an aryl, a substituted aryl, a heteroaryl, a substituted heteroaryl, a carboxyaryl, a heterocyclic group, a substituted heterocyclic group, a fused cycloalkyl, a substituted fused cycloalkyl, a fused heterocyclic group, a substituted fused heterocyclic group, a fused aryl, a substituted fused aryl, a fused heteroaryl, a substituted fused heteroaryl ring, or any combination thereof, optionally further comprising a hydroxy, an alkoxy, an aryloxy, an oxo, an ester, an ether, an amine, an azo, an azido, a nitro, an imine, an isothionate, a carbonyl, a peroxide, a halogen, a formyl, an acyl, a carboxy, an amido, a carbamoyl, a guanidino, a ureido, a amidino, a thiol, a mercapto, a sulfinyl, a sulfonyl and/or a sulfonamide, and wherein R2 is

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The invention is additionally directed to methods of inhibiting COX-2 in a mammal. The methods comprise administering an inhibitor of prostaglandin transporter activity to the mammal.

The present invention is further directed to methods of treating pain or inflammation in a mammal. The methods comprise administering an inhibitor of prostaglandin transporter activity to the mammal.

The invention is also directed to compounds that inhibit prostaglandin transporter activity, where the compound is

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The invention is further directed to pharmaceutical compositions comprising any of the above-described compounds in a pharmaceutically acceptable excipient.

Additionally, the invention is directed to the use of a COX-2-inhibiting effective amount of Compound 1, or a pharmaceutically acceptable sail, ester, or tautomer thereof, for the manufacture of a medicament for the treatment of a disorder or condition at least partially mediated by COX-2 in a mammal. Here, Compound 1 is

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BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the chemical structures of the main scaffolds used to identify prostaglandin transporter inhibitors.

FIG. 2 shows the structures of the three most potent TGBz compounds and their degree of PGT inhibition at 25 μM.

FIG. 3 is a graph of results of a kinetic study to determine the inhibitory effect of TGBz T34 on PGT-mediated PGE₂uptake. The TGBz T34 inhibition constant and its mode of inhibition were determined by varying PGE₂concentration at fixed levels of TGBz T34 equal to 0 μM (circle), 3 μM (square), and 6 μM (diamond). Double-reciprocal plots for TGBz T34 inhibition demonstrate that TGBz T34 is a competitive inhibitor of PGT with a K_ivalue of 3.7±0.2 μM.

FIG. 4 is graphs and a chart of experimental results showing the time course of PGE₂uptake by of MDCK cells stably expressing PGT. In the experiment of panel A, TGBz T34 was added at various concentrations. TGBz T34 was added at the beginning of each time course at 0 μM (square), 1 μM (circle), 4 μM (diamond), 10 μM (up triangle), 25 μM (down triangle). In the experiment of panel B, the cells were without TGBz T34 (open circle) and with 25 μM TGBz T34 added at the time point at which intracellular PGE₂reached its peak level (solid circle). In the experiment of panel C, 25 μM TGBz T34 was added at different time points on the uptake time course: 3 minutes (up triangle), 6 minutes (down triangle), 9 minutes (diamond), 20 minutes (circle), and 35 minutes (square). Panel D shows initial PGE₂efflux velocities (V_i) and their corresponding intracellular PGE₂concentrations as obtained from the data of panel C.

FIG. 5 shows the full set of R1 structures utilized in the library.

FIG. 6 shows the full set of R2 structures utilized in the library.

FIG. 7 shows the three of the six initial compounds that were not initially chosen for further study.

FIG. 8 is a diagram showing a model for PGT regulation of PGE₂receptor (EP) expression.

FIG. 9 is a graph of experimental results showing the modulation of high-affinity EP₄binding sites by PGT in MDCK cells. In each analysis, non-specific PGE₂binding has been subtracted out.

FIG. 10 is a graph of experimental results also showing the modulation of high-affinity EP₄binding sites by PGT in MDCK cells. This is a similar study to that of FIG. 9, except that in this case PGT expression is either permitted, or is suppressed with doxycycline (Dox). Data points on the x-axis at [PGE₂]=55 nM indicate absence of PGE₂binding without EP₄transfection.

FIG. 11 shows the structure of TGBZ T26, the most potent PGT inhibitor identified in this study.

FIG. 12 is a graph of experimental results showing a dose response of T26 IV injections on systemic BP in anesthetized rats of the control strain (WKY) and the Spontaneously Hypertensive Rat (SHR) strain.

FIG. 13 shows the knock-down of PGT mRNA expression in Swiss 3T3 fibroblasts. At each of the two time points, the left curve depicts amplification of β-actin in both control and in PGT siRNA transfected cells; the middle curves represent amplification of PGT mPGT in RNA in control cells; and the right curves represent amplification of PGT mPGT mRNA derived from siRNA-transfected cells. A shift to the right in a curve means that a higher number of PCR cycles is required for amplification, indicating a lower starting concentration of template (i.e. PGT mRNA).

FIG. 14 is a graph of experimental results showing the inhibition of Cox-2 in Swiss 3T3 cells by suppression of PGT.

FIG. 15 is a graph showing that T26A increases endogenous PGE₂in circulating blood of rats.

FIG. 16 is a graph showing that T26A reduces the degradation of exogenously-administered PGE₂.

FIG. 17 is a graph showing that chronic oral administration of T26A increases endogenous PGD₂in whole blood.

FIG. 18 is a graph showing that chronic oral administration of T26A increases PGE₂excretion in urine.

FIG. 19 is a graph showing that chronic oral administration of T26A increases bleeding time.

FIG. 20 is four graphs showing that chronic oral administration of T26A does not have any apparent effects on water and food intake, body weight or daily urine volume.

FIG. 21 is micrographs of stained tissue sections showing that chronic oral administration of T26 apparently does not cause any pathological damage to the gastrointestinal tract.

DETAILED DESCRIPTION OF THE INVENTION
Abbreviations

GFP, green fluorescence protein; MRP4, Multi Drug Resistance Protein 4; OAT, Organic Anion Transporter

Accordingly, the inventors have identified compounds that inhibit mammalian prostaglandin transporter (PGT) activity. See Examples. These compounds are useful, e.g., in the treatment of disorders that are at least partially mediated by excessive PGT activity. Without being bound by any particular mechanism, it is believed that prostaglandin transport prevents prostaglandins from binding to EP₄receptors on the cells surface (FIG. 8). That binding does take place if PGT is inhibited, causing the EP₄receptors to become internalized and desensitizing the cell to later prostaglandin binding. Thus, inhibiting prostaglandin transport is believed to inhibit the effect of a subsequent exposure of the cell to induced prostaglandins by desensitizing cells to the prostaglandin effects.

Thus, the present invention is directed to methods of inhibiting prostaglandin transporter (PGT) activity in a mammal. The methods comprise administering a compound to the mammal effective to inhibit PGT activity. In these methods, the compound has Formula I:

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As used herein, unless otherwise specifically designated, a “substituted” moiety is a moiety that further comprises a halogen (F, Cl, Br, or I), a hydroxy, a C₁-C₃alkyl, a C₁-C₃alkoxy, an azido, an amino, a nitro, a keto, a formyl, an azo, a carbonyl, a carboxy, an amido, a carbamoyl, a thiol, a mercapto, a sulfinyl, or a sulfonyl.

Preferably, R comprises a carboxyl or phenol group. More preferably, R is a C₁-C₁₀straight or branched alkyl, a phenyl, a fused aryl, a fused heteroaryl, or any combination thereof, optionally substituted with a halogen, a carboxyl, an amino, a nitro, a SCH₃, a hydroxyl, a C₁-C₃alkoxy, C₁-C₃straight or branched alkyl, an azido, or a combination thereof. Even more preferably, R is a C₁-C₅straight or branched alkyl, a phenyl, a fused aryl, or a combination of a C₁-C₅straight or branched alkyl and a phenyl, optionally substituted with a halogen, one or more hydroxyls, a methoxy, a nitro, a carboxy, or a combination thereof. Still more preferably, R is

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Most preferably, the compound is

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The inventors have also discovered that inhibiting prostaglandin transport inhibits COX-2 expression. See Example 2. Thus, the mammal in these methods is preferably a human suffering from a disease or disorder at least partially mediated by a cyclooxygenase-2. More preferably, the disease or disorder involves pain and/or inflammation. Non-limiting examples of diseases or disorders at least partially mediated by a cyclooxygenase-2 are arthritis, fever, common cold, hypertension, glaucoma, slow wound healing, slow initiation of labor, dysmenorrhea, menstrual cramps, inflammatory bowel disease, Crohn's disease, emphysema, acute respiratory distress syndrome, asthma, bronchitis, chronic obstructive pulmonary disease, Alzheimer's disease, organ transplant toxicity, cachexia, allergic reactions, allergic contact hypersensitivity, cancer, tissue ulceration, peptic ulcers, gastritis, regional enteritis, ulcerative colitis, diverticulitis, recurrent gastrointestinal lesion, gastrointestinal bleeding, coagulation, anemia, synovitis, gout, ankylosing spondylitis, restenosis, periodontal disease, epidermolysis bullosa, osteoporosis, loosening of artificial joint implants, atherosclerosis, aortic aneurysm, periarteritis nodosa, congestive heart failure, myocardial infarction, stroke, cerebral ischemia, head trauma, spinal cord injury, neuralgia, neuro-degenerative disorders, autoimmune disorders, Huntington's disease, Parkinson's disease, migraine, depression, peripheral neuropathy, pain, gingivitis, cerebral amyloid angiopathy, nootropic or cognition enhancement, amyotrophic lateral sclerosis, multiple sclerosis, ocular angiogenesis, corneal injury, macular degeneration, conjunctivitis, abnormal wound healing, muscle or joint sprains or strains, tendonitis, skin disorders, myasthenia gravis, polymyositis, myositis, bursitis, burns, diabetes, tumor invasion, tumor growth, tumor metastasis, corneal scarring, scleritis, immunodeficiency diseases, sepsis, premature labor, hyporothrombinemia, hemophilia, thyroiditis, sarcoidosis, Behcet's syndrome, hypersensitivity, kidney disease, rickettsial infections, protozoan diseases, reproductive disorders or septic shock.

The invention is also directed to methods of determining whether a test compound is an inhibitor of a prostaglandin transporter. These methods comprise contacting the test compound with the prostaglandin transporter, then determining whether the prostaglandin transporter has less transporter activity when contacted with the test compound than when not so contacted. The test compound in these methods is

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These methods could employ any known procedures for determining prostaglandin transporter activity, e.g., those used in the Examples.

In these methods, R1 is preferably

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The invention is further directed to compounds that inhibit prostaglandin transporter activity. These compounds are

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With these, or any other set of PGT inhibitors identified herein (unless specifically excluded), it is understood that each of the above compounds individually could have different effects on PGT and prostaglandin metabolism or signaling, or side effects in particular species or individuals within any species. The skilled artisan could select the most preferred of any of the inhibitors for any particular application without undue experimentation.

The invention is additionally directed to pharmaceutical compositions comprising any of the above compounds in a pharmaceutically acceptable excipient.

By “pharmaceutically acceptable” it is meant a material that (i) is compatible with the other ingredients of the composition without rendering the composition unsuitable for its intended purpose, and (ii) is suitable for use with subjects as provided herein without undue adverse side effects (such as toxicity, irritation, and allergic response). Side effects are “undue” when their risk outweighs the benefit provided by the composition. Non-limiting examples of pharmaceutically acceptable carriers include, without limitation, any of the standard pharmaceutical carriers such as phosphate buffered saline solutions, water, emulsions such as oil/water emulsions, microemulsions, and the like.

The above-described compounds can be formulated without undue experimentation for administration to a mammal, including humans, as appropriate for the particular application. Additionally, proper dosages of the compositions can be determined without undue experimentation using standard dose-response protocols.

Accordingly, the compositions designed for oral, lingual, sublingual, buccal and intrabuccal administration can be made without undue experimentation by means well known in the art, for example with an inert diluent or with an edible carrier. The compositions may be enclosed in gelatin capsules or compressed into tablets. For the purpose of oral therapeutic administration, the pharmaceutical compositions of the present invention may be incorporated with excipients and used in the form of tablets, troches, capsules, elixirs, suspensions, syrups, wafers, chewing gums and the like.

Tablets, pills, capsules, troches and the like may also contain binders, recipients, disintegrating agent, lubricants, sweetening agents, and flavoring agents. Some examples of binders include microcrystalline cellulose, gum tragacanth or gelatin. Examples of excipients include starch or lactose. Some examples of disintegrating agents include alginic acid, cornstarch and the like. Examples of lubricants include magnesium stearate or potassium stearate. An example of a glidant is colloidal silicon dioxide. Some examples of sweetening agents include sucrose, saccharin and the like. Examples of flavoring agents include peppermint, methyl salicylate, orange flavoring and the like. Materials used in preparing these various compositions should be pharmaceutically pure and nontoxic in the amounts used.

The compounds can easily be administered parenterally such as for example, by intravenous, intramuscular, initrathecal or subcutaneous injection. Parenteral administration can be accomplished by incorporating the compounds into a solution or suspension. Such solutions or suspensions may also include sterile diluents such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents. Parenteral formulations may also include antibacterial agents such as for example, benzyl alcohol or methyl parabens, antioxidants such as for example, ascorbic acid or sodium bisulfite and chelating agents such as EDTA. Buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose may also be added. The parenteral preparation can be enclosed in ampules, disposable syringes or multiple dose vials made of glass or plastic.

Rectal administration includes administering the compound, in a pharmaceutical composition, into the rectum or large intestine. This can be accomplished using suppositories or enemas. Suppository formulations can easily be made by methods known in the art. For example, suppository formulations can be prepared by heating glycerin to about 120° C., dissolving the composition in the glycerin, mixing the heated glycerin after which purified water may be added, and pouring the hot mixture into a suppository mold.

Transdermal administration includes percutaneous absorption of the composition through the skin. Transdermal formulations include patches (such as the well-known nicotine patch), ointments, creams, gels, salves and the like.

The present invention includes nasally administering to the mammal a therapeutically effective amount of the compound. As used herein, nasally administering or nasal administration includes administering the compound to the mucous membranes of the nasal passage or nasal cavity of the patient. As used herein, pharmaceutical compositions for nasal administration of the compound include therapeutically effective amounts of the compound prepared by well-known methods to be administered, for example, as a nasal spray, nasal drop, suspension, gel, ointment, cream or powder. Administration of the compound may also take place using a nasal tampon or nasal sponge.

Where the compound is administered peripherally such that it must cross the blood-brain barrier, the compound is preferably formulated in a pharmaceutical composition that enhances the ability of the compound to cross the blood-brain barrier of the mammal. Such formulations are known in the art and include lipophilic compounds to promote absorption. Uptake of non-lipophilic compounds can be enhanced by combination with a lipophilic substance. Lipophilic substances that can enhance delivery of the compound across the nasal mucus include but are not limited to fatty acids (e.g., palmitic acid), gangliosides (e.g., GM-1), phospholipids (e.g., phosphatidylserine), and emulsifiers (e.g., polysorbate 80), bile salts such as sodium deoxycholate, and detergent-like substances including, for example, polysorbate 80 such as Tween™, octoxynol such as Triton™ X-100, and sodium tauro-24,25-dihydrofusidate (STDHF). See Lee et al., Biopharm., April 1988 issue: 3037.

In particular embodiments of the invention, the compound is combined with micelles comprised of lipophilic substances. Such micelles can modify the permeability of the nasal membrane to enhance absorption of the compound. Suitable lipophilic micelles include without limitation gangliosides (e.g., GM-1 ganglioside), and phospholipids (e.g., phosphatidylserine). Bile salts and their derivatives and detergent-like substances can also be included in the micelle formulation. The compound can be combined with one or several types of micelles, and can further be contained within the micelles or associated with their surface.

Alternatively, the compound can be combined with liposomes (lipid vesicles) to enhance absorption. The compound can be contained or dissolved within the liposome and/or associated with its surface. Suitable liposomes include phospholipids (e.g., phosphatidylserine) and/or gangliosides (e.g., GM-1). For methods to make phospholipid vesicles, see for example, U.S. Pat. No. 4,921,706 to Roberts et al., and U.S. Pat. No. 4,895,452 to Yiournas et al. Bile salts and their derivatives and detergent-like substances can also be included in the liposome formulation.

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Preferably, the mammal is a human suffering from a disease or disorder at least partially mediated by a cyclooxygenase-2. More preferably, the disease or disorder involves pain and/or inflammation. Non-limiting examples of diseases or disorders at least partially mediated by a cyclooxygenase-2 are arthritis, fever, common cold, hypertension, glaucoma, slow wound healing, slow initiation of labor, dysmenorrhea, menstrual cramps, inflammatory bowel disease, Crohn's disease, emphysema, acute respiratory distress syndrome, asthma, bronchitis, chronic obstructive pulmonary disease, Alzheimer's disease, organ transplant toxicity, cachexia, allergic reactions, allergic contact hypersensitivity, cancer, tissue ulceration, peptic ulcers, gastritis, regional enteritis, ulcerative colitis, diverticulitis, recurrent gastrointestinal lesion, gastrointestinal bleeding, coagulation, anemia, synovitis, gout, ankylosing spondylitis, restenosis, periodontal disease, epidermolysis bullosa, osteoporosis, loosening of artificial joint implants, atherosclerosis, aortic aneurysm, periarteritis nodosa, congestive heart failure, myocardial infarction, stroke, cerebral ischemia, head trauma, spinal cord injury, neuralgia, neuro-degenerative disorders, autoimmune disorders, Huntington's disease, Parkinson's disease, migraine, depression, peripheral neuropathy, pain, gingivitis, cerebral amyloid angiopathy, nootropic or cognition enhancement, amyotrophic lateral sclerosis, multiple sclerosis, ocular angiogenesis, corneal injury, macular degeneration, conjunctivitis, abnormal wound healing, muscle or joint sprains or strains, tendonitis, skin disorders, myasthenia gravis, polymyositis, myositis, bursitis, burns, diabetes, tumor invasion, tumor growth, tumor metastasis, corneal scarring, scleritis, immunodeficiency diseases, sepsis, premature labor, hyporothrombinemia, hemophilia, thyroiditis, sarcoidosis, Behcet's syndrome, hypersensitivity, kidney disease, rickettsial infections, protozoan diseases, reproductive disorders or septic shock.

Additionally, the invention is directed to methods of determining whether a test compound is an inhibitor of a prostaglandin transporter. The methods comprise contacting the test compound with the prostaglandin transporter, then determining whether the prostaglandin transporter has less transporter activity when contacted with the test compound than when no so contacted. In these methods, the test compound is

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Preferably in these methods, R3 is

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Preferred R1 moieties are

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Additionally, preferred R2 moieties are

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Examples of preferred R3 moieties are

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Examples of preferred R1 moieties are

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The inventors have also discovered that inhibition of PGT causes a reduction in cyclooxygenase-2 (COX-2) mRNA production, function, and protein (Example 2). Thus, the invention is additionally directed to methods of inhibiting COX-2 in a mammal. The methods comprise administering an inhibitor of prostaglandin transporter activity to the mammal.

The inhibitor of prostaglandin transporter activity in these methods can be any compound that inhibits expression or transporter activity of the PGT. Thus, for these methods, the inhibitor can prevent translation of the protein. Examples of such inhibitors are antisense nucleic acids, ribozymes or siRNA, where the antisense nucleic acid, the ribozyme or the siRNA is specific for the mRNA of the prostaglandin transporter. The skilled artisan could design and produce such inhibitors without undue experimentation.

The inhibitor in these methods can also be an antibody or aptamer that specifically inhibits the prostaglandin transporter. Production of these inhibitors is also within the skill of the art.

Preferably in these methods, the inhibitor is a compound having Formula I:

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More preferably, R1 is

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Preferably, R2 comprises a carboxyl or phenol group. More preferably, R2 is a C₁-C₁₀straight or branched alkyl, a phenyl, a fused aryl, a fused heteroaryl, or any combination thereof, optionally substituted with a halogen, a carboxyl, an amino, a nitro, a SCH₃, a hydroxyl, a C₁-C₃alkoxy, C₁-C₃straight or branched alkyl, an azido, or a combination thereof. Even more preferably, R2 is a C₁-C₅straight or branched alkyl, a phenyl, a fused aryl, or a combination of a C₁-C₈straight or branched alkyl and a phenyl, optionally substituted with a halogen, one or more hydroxyls, a methoxy, a nitro, a carboxy, or a combination thereof. Still more preferably, R2 is

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Most preferably, the compound in these methods is

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In preferred aspects of these methods, the mammal is a human suffering from a disease or disorder at least partially mediated by a cyclooxygenase-2. More preferably, the disease or disorder involves pain and/or inflammation. Non-limiting examples of diseases or disorders at least partially mediated by a cyclooxygenase-2 are arthritis, fever, common cold, hypertension, glaucoma, slow wound healing, slow initiation of labor, dysmenorrhea, menstrual cramps, inflammatory bowel disease, Crohn's disease, emphysema, acute respiratory distress syndrome, asthma, bronchitis, chronic obstructive pulmonary disease, Alzheimer's disease, organ transplant toxicity, cachexia, allergic reactions, allergic contact hypersensitivity, cancer, tissue ulceration, peptic ulcers, gastritis, regional enteritis, ulcerative colitis, diverticulitis, recurrent gastrointestinal lesion, gastrointestinal bleeding, coagulation, anemia, synovitis, gout, ankylosing spondylitis, restenosis, periodontal disease, epidermolysis bullosa, osteoporosis, loosening of artificial joint implants, atherosclerosis, aortic aneurysm, periarteritis nodosa, congestive heart failure, myocardial infarction, stroke, cerebral ischemia, head trauma, spinal cord injury, neuralgia, neuro-degenerative disorders, autoimmune disorders, Huntington's disease, Parkinson's disease, migraine, depression, peripheral neuropathy, pain, gingivitis, cerebral amyloid angiopathy, nootropic or cognition enhancement, amyotrophic lateral sclerosis, multiple sclerosis, ocular angiogenesis, corneal injury, macular degeneration, conjunctivitis, abnormal wound healing, muscle or joint sprains or strains, tendonitis, skin disorders, myasthenia gravis, polymyositis, myositis, bursitis, burns, diabetes, tumor invasion, tumor growth, tumor metastasis, corneal scarring, scleritis, immunodeficiency diseases, sepsis, premature labor, hyporothrombinemia, hemophilia, thyroiditis, sarcoidosis, Behcet's syndrome, hypersensitivity, kidney disease, rickettsial infections, protozoan diseases, reproductive disorders or septic shock.

The present invention is further directed to methods of treating pain or inflammation in a mammal. The methods comprise administering an inhibitor of prostaglandin transporter activity to the mammal. Since PGT inhibition also inhibits COX-2, and inhibition of COX-2 is a recognized treatment of pain or inflammation, PGT inhibitors are useful for treating pain or inflammation. As used herein, unless otherwise specified, “pain or inflammation” is equivalent to “pain or inflammation or both” and “pain and/or inflammation”.

In these methods, the inhibitor of PGT activity can function by inhibiting production of PGT, e.g., through the use of antisense nucleic acids, ribozymes, or siRNA (Example 2) that are specific for the mRNA of the prostaglandin transporter. Methods of making and testing such compounds are within the skill of the art.

The inhibitor of PGT activity can also function by inhibiting the function of the PGT, preferably by binding to the PGT, making the substrate-interacting site inaccessible to the substrate. Examples of such inhibitors are antibodies or aptamers that specifically inhibit the prostaglandin transporter. Preferably, the inhibitor is a compound having Formula I:

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Preferably in these methods, the R1 of Formula I is

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Most preferably, the compound is

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The invention is also directed to compounds that inhibit prostaglandin transporter activity, where the compound is

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The invention is further directed to pharmaceutical compositions comprising any of the above-described compounds in a pharmaceutically acceptable excipient.

Additionally, the invention is directed to the use of a COX-2-inhibiting effective amount of Compound 1, or a pharmaceutically acceptable salt, ester, or tautomer thereof, for the manufacture of a medicament for the treatment of a disorder or condition at least partially mediated by COX-2 in a mammal. Here, Compound 1 is

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or a pharmaceutically acceptable salt, ester, or tautomer thereof, wherein R1 and R2 are independently a C₁-C₁₅is straight or branched alkyl, a substituted alkyl, a cycloalkyl, a carboxyalkyl, a substituted cycloalkyl, a C₁-C₁₅straight or branched alkenyl, a substituted alkenyl, a cycloalkenyl, a substituted cycloalkenyl, a C₁-C₁₅straight or branched alkinyl, a substituted alkinyl, a cycloalkinyl, a substituted cycloalkinyl, a C₁-C₁₀straight or branched ether, a substituted ether, a cycloether, an ester, an amide, an acetyl, an aminal, an anhydride, an aryl, a substituted aryl, a heteroaryl, a substituted heteroaryl, a carboxyaryl, a heterocyclic group, a substituted heterocyclic group, a fused cycloalkyl, a substituted fused cycloalkyl, a fused heterocyclic group, a substituted fused heterocyclic group, a fused aryl, a substituted fused aryl, a fused heteroaryl, a substituted fused heteroaryl ring, or any combination thereof, optionally further comprising a hydroxy, an alkoxy, an aryloxy, an oxo, an ester, an ether, an amine, an azo, an azido, a nitro, an imine, an isothionate, a carbonyl, a peroxide, a halogen, a formyl, an acyl, a carboxy, an amido, a carbamoyl, a guanidino, a ureido, a amidino, a thiol, a mercapto, a sulfinyl, a sulfonyl and/or a sulfonamide.

Preferably, R1 is

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Most preferably, the compound of Formula I is

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Preferred embodiments of the invention are described in the following examples. Other embodiments within the scope of the claims herein will be apparent to one skilled in the art from consideration of the specification or practice of the invention as disclosed herein. It is intended that the specification, together with the examples, be considered exemplary only, with the scope and spirit of the invention being indicated by the claims which follow the examples.

Example 1
Identification of a New Class of PGT Inhibitors and Characterization or Their Biological Effects on PGE₂Transport

Example Summary

Prostaglandins (PGs) are involved in several major signaling pathways. Their effects are terminated when they are transported across cell membranes and oxidized intracellularly. The transport step of PG metabolism is carried out by the prostaglandin transporter (PGT). Inhibition of PGT would therefore be expected to change local or circulating concentrations of prostaglandins, and thus their biological effects. To develop PGT-specific inhibitors with high-affinity, a library of triazine compounds was designed, and 1,842 small molecules were screened by using MDCK cells stably expressing rat PGT. Several effective PGT inhibitors were found. Among them, the most potent inhibitor was TGBz T34, with a K_iof 3.7±0.2 μM. These inhibitors allowed the isolation of the efflux process of PGE₂and the demonstration that PGT does not transport PGE₂outwardly under physiological conditions.

Introduction

Prostaglandins (PGs) are local signaling molecules that trigger pain, fever, and inflammation, among other physiological effects. PG signaling is mediated by cell-surface PG receptors. The PG uptake carrier PGT, which is expressed on the surface of cells that synthesize PGs, removes PGs from the pericellular fluid and thereby determines the set-point of PG receptors at the cell surface.

To develop high-affinity, PGT-specific inhibitors, compounds from a triazine library were screened. Using Madin Darby Canine Kidney (MDCK) cells stably expressing PGT (Endo et al., 2002), screening of 1,842 small molecules yielded several effective inhibitors. The most potent inhibitor in this group of compounds, TGBz T34, has a K_iof 3.7±0.2 μM. This compound also permitted isolation of the efflux process of PGE₂transport and the demonstration that PGE₂influx and efflux are mediated by separate processes.

Methods

Materials. The cell lines used in this study were 3T3 cells that express endogenous PGT, and MDCK cells stably transfected with the GFP-tagged PGT (Endo et al., 2002). Tritium labeled PGE₂([³H]PGE₂) was purchased from Perkin Elmer. Unlabeled PGE₂was obtained from Cayman.

Synthesis of 1842 small molecule compounds. The methods and procedures for synthesis of 1842 compounds were reported elsewhere (Moon et al., 2002; Bork et al., 2003a; Bork et al., 2003b; Khersonsky et al., 2003; Uttamchandani et al., 2004).

PGE₂Transport Measurement. MDCK or 3T3 cells were seeded at 15-20% confluence on 24-well plates. The day on which the cells were seeded was considered day 1. PGE₂uptake experiments were conducted on day 4. All of the PGE₂uptake experiments were conducted at room temperature. On day 4, cells were washed twice with Waymouth buffer (135 mM NaCl, 13 mM H-Hepes, 13 mM Na-Hepes, 2.5 mM CaCl₂, 1.2 mM MgCl₂, 0.8 mM MgSO₄, 5 mM KCl, and 28 mM D-glucose). Then 200 μL of Waymouth buffer containing [³H]PGE₂was added to each well. At the designated time, the uptake of [³H]PGE₂was stopped by aspiration of uptake buffer; this was followed by immediate washing twice with 500 μL of chilled Waymouth buffer. Cells were then lysed with 100 μL lysis buffer containing 0.25% SDS and 0.05 N NaOH. 1.5 mL of scintillation solution was added to each well, and intracellular [³H]PGE₂was counted by MicroBeta Counter.

For preliminary screening of the compounds, 25 μL of Waymouth buffer containing small organic compounds were added to each well; this was immediately followed by the addition of 175 μL of Waymouth buffer containing [³H]PGE₂. In each well, the total volume of uptake medium was 200 μL. Organic compounds were first dissolved in DMSO and then diluted in Waymouth buffer. The percent inhibition of [³H]PGE₂uptake by compounds was calculated as [(uptake_vehicle−uptake_inhibitor)÷(uptake_vehicle)]×100.

Measurements of K_ivalues. The initial velocities at various initial extracellular concentrations of PGE₂were determined from the PGE₂uptake in the first 2 minutes; these were linear over the time course of PGE₂uptake. K_ivalues were obtained by curve fitting the reciprocal of initial velocities of PGE₂uptake versus the reciprocal of extracellular PGE₂concentrations at various concentrations of the inhibitors. At low PGE₂concentrations, the extracellular concentrations were taken as ³H labeled PGE₂, which has a specific activity of 500 μCi/mol. At high concentrations of PGE₂, a mixture of ³H labeled and unlabeled PGE₂was made to a final specific activity of 25 μCi/mol.

Results.

Screening of small molecules for inhibition of PGE₂uptake. The small molecule triazine library compounds (1,842 members) were synthesized following reported procedures (Moon et al., 2002; Bork et al., 2003a; Bork et al., 2003b; Khersonsky et al., 2003; Uttamchandani et al., 2004). The main scaffolds of the compounds are depicted in FIG. 1 with codes of AA, BN, EA, RT, TF, and TGBz. The full structural information on R1 and R2 groups is provided in FIGS. 5 and 6. Among the 1,842 compounds tested, the six compounds with the highest inhibitory activities were all from the TGBz scaffold. The T substituent at the R1 position and an acidic group (COOH or phenol) at the R2 position constitute important motifs for activity.

Determination of K_ivalues of inhibitors and their modes of inhibition. Of the six initial compounds, TGBz T34, T07, and T41 (FIG. 2) were chosen to further determine their inhibition kinetic parameters. Structures of the other three compounds are given in FIG. 7. The inhibition constant of TGBz T34 and its mode of inhibition, as determined by varying the PGE₂concentrations at fixed levels of TGBz T34, are shown in FIG. 3. The pattern was characteristic for competitive inhibition. The same experiments were conducted for TGBz T41 and T07; the K_ivalues are listed in Table 1. All of these compounds are competitive inhibitors of PGT. TGBz T34 is the most potent inhibitor with a K_iof 3.7±0.2 μM. In separate experiments, when cells were pre-incubated in TGBz T34 for 10 or 20 minutes, the K_iwas not significantly different from that obtained by adding TGBz T34 simultaneously with PGE₂(0 min pre-incubation K_i=1.22 μM; 10 min pre-incubation K_i=1.63 μM; 20 min pre-incubation K_i=1.41 μM, NS to each other). These data suggest that there is no significant time dependency of binding of the inhibitor to PGT.

TABLE I

K_iValues of TGBz Inhibitors of PGT

Compounds
K_i(μM)

T34
3.7 ± 0.2

T41
6.2 ± 0.7

T07
12.5 ± 1.5

TGBz T34 specifically inhibits PGE₂uptake by PGT. A typical time course of PGE₂uptake in the absence of inhibitor is shown in FIG. 4A (squares). In the absence of TGBz T34, intracellular PGE₂rapidly accumulated, reaching a peak within 9 or 10 minutes. After this overshoot, a plateau was obtained, indicating that the rate of uptake equaled the rate of efflux. These data are similar to those previously published (Chan et al., 1998; Chan et al., 2002).

To further test the inhibition effect of TGBz T34, the time course of PGE₂uptake was measured in the presence of various concentrations of TGBz T34 added at the beginning of uptake. As shown in FIG. 4A, as the concentration of TGBz T34 increased, the peak level of intracellular PGE₂accumulation decreased and the time point for reaching the peak PGE₂level shifted, such that it took a shorter time for intracellular PGE₂to reach its peak level at higher concentrations of TGBz T34. Also, as the concentration of TGBz T34 increased, the overshoot phenomenon diminished. When the concentration of TGBz T34 was 25 μM, i.e. 8 fold higher than its K_i, the overshoot phenomenon completely disappeared. The residual uptake reflects PGE₂entry by diffusion; it is similar to the curve of PGE₂uptake by wild-type MDCK cells before they were transfected with PGT (Endo et al., 2002). Similar overshoot data and inhibition by TGBz T34 were obtained in Swiss 3T3 cells expressing endogenous PGT (data not shown).

PGT does not export PGE₂. Since the discovery of PGT, the issue of whether it transports PGs in both directions has been unsettled (Chan et al., 1998; Schuster, 2002; Banu et al., 2003). To resolve this issue, TGBz T34 was applied after loading intracellular PGE₂to a peak level (9 minutes) so as to block all PGE₂transport by PGT, and then the efflux of PGE₂was monitored. As shown in FIG. 4B, addition of TGBz T34 at 25 μM induced a rapid depletion of intracellular PGE₂. Intracellular PGE₂fell to baseline within 5 minutes and remained at that level for the rest of the time course. When there was no addition of T34, intracellular PGE₂stayed at a much higher level. This result strongly suggests that PGT does not participate in PGE₂efflux. Instead, efflux occurs by either simple diffusion or by a combination of diffusion and another very low-affinity carrier.

PGE₂efflux occurs by simple diffusion. We used TGBz T34 to further isolate the efflux process of PGE₂. As shown in FIG. 4C, we allowed PGE₂uptake to proceed to different time points (3, 6, 9, 20, and 35 minutes) and then added 25 μM T34 to stop the accumulation. The intracellular concentrations of PGE₂at different time points were calculated by dividing the total amount of intracellular PGE₂on the dish by the total volume of cells, based on cell number counts and published individual cell volume (Schneider et al., 2000; Hill et al., 2004). Since the addition of inhibitor involved removing extracellular tracer PGE₂, the intracellular PGE₂concentration at the point of inhibitor addition approximates the outwardly-directed PGE₂gradient. Initial PGE₂efflux rates at various PGE₂outward gradients are shown in FIG. 4D.

Initial PGE₂efflux rates from FIGS. 4C and 4D were linear as a function of the outwardly-directed PGE₂gradients over the range of 0 to 30 nM, with a “y” intercept not significantly different from zero (efflux rate=[(0.0106)(gradient)]+0.048, r²=0.98, p<0.05). This linearity held true even when the intracellular PGE₂concentration was extended to almost 800 nM, i.e. 10 fold the K_mof PGT for PGE₂(data not shown). From the slope of the relationship, we generated a range of permeability coefficients for PGE₂efflux of 1.2 to 5.3×10⁻⁶cm/sec, based on the range of MDCK cell volumes reported in the literature (Schneider et al., 2000; Hill et al., 2004). Using wild-type MDCK cells that do not express PGT, a permeability coefficient for PGE₂influx (by simple diffusion) of 0.45×10⁻⁶cm/sec was obtained (data not shown). The ratio of the influx-to-efflux permeability coefficients was thus in the range 2.7 to 11.7.

Discussion

Organic dyes and nonsteroidal anti-inflammatory drugs have been known to inhibit PGT for some time (Kanai et al., 1995). This is the first report of a new class of PGT inhibitors developed by screening small molecules. The compound library was built by solid-phase combinatorial chemistry and screened by MicroBeta scintillation counting on multi-well plates. This strategy led to the discovery of a PGT inhibitor, TGBz T34, with a K_iof 3.7±0.2 μM, after screening fewer than 2000 compounds. At 25 μM, TGBz T34 exerted full inhibition of PGE₂transport by PGT. Double reciprocal analysis revealed that T34 is a competitive inhibitor of PGT. Because T34 eliminated PGT transport activity rapidly, it probably inhibits PGT directly rather than indirectly via metabolic effects.

The K_iof TGBz T34 is similar to that of bromcresol green (Kanai et al., 1995). TGBz T34 has the potential to be improved because there are three moieties around the scaffold that can be modified. Native substrates of PGT all possess a COOH group and are negatively charged at physiological pH (Schuster, 1998). The carboxylic group at carbon 1 is critical for PG binding to PGT (Eling et al., 1977; Schuster et al., 2000), which is probably why group B (FIG. 7) was associated with inhibition.

Some investigators have hypothesized that PGT represents the mechanism by which PGs efflux from cells (Funk, 2001; Banu et al., 2003), whereas others have invoked active pumps such as MRP4 (Reid et al., 2003). Identification of these inhibitors enabled an investigation into the mechanism of PGE₂transport in a more refined way. As reported previously (Chan et al., 1998; Schuster, 2002) and in the present study, a normal time course of PGE₂transport is divided into three phases (FIG. 4). Phase 1 is rapid uptake, phase 2 is overshoot, and phase 3 is an equilibrium phase. Addition of T34 at the point of peak intracellular PGE₂accumulation demonstrated that PGE₂efflux is ongoing during PGT-mediated uptake (FIG. 4) i.e. the accumulation of intracellular PGE₂in phase 1, and the maintenance of the equilibrium in phase 3, are due to the active pumping of PGE₂into the cells by PGT against a background efflux.

Using TGBz T34 the isolation of the components of PGE₂efflux was possible. After loading cells with PGE₂and blocking PGT-mediated uptake with T34, the PGE₂efflux rate was linear as a function of the estimated outwardly-directed PG gradient, even at high concentrations. These data indicate that PGE₂efflux, at least from the compartment loaded by PGT, most likely occurred by simple diffusion.

The hypothesis that PGE₂efflux occurs by simple diffusion is further supported by our calculated permeability coefficients. At physiological pH, PGs are negatively charged. Because the cell interior is electrically negative, the electrical driving force for simple diffusion is in favor of PGE₂efflux. The theoretical ratio of the permeability coefficients for diffusional efflux compared to diffusional influx, based on the membrane potential, is in the range of 2-11 (Schuster, 2002). The ratios we generated agree with this range. Taken together, our data support a model of PGE₂transport as a pump (PGT-mediated influx)−leak (diffusional efflux) system.

In summary, reported here is the development of a new class of PGT inhibitors by screening a library of small molecules. The most potent of these allowed the clarification of the mechanisms for influx and efflux of PGE₂. This compound and others should form the basis for further pharmacological investigation of PG transport and should serve as lead compounds in developing therapeutic agents.

Example 2
Additional PGT Inhibitors and Effect of PGT Inhibition on COX-2 Activity

Structurally agnostic screening was carried out with ˜2,000 organic small molecules that were modified from lead compounds T34 and T41, described in Example 1. The most effective, compound TGBZ T26A, competitively inhibits PGT at a K_i=300 nM.

Table 2 provides the PGT inhibitors from this screening that showed greater than 50% inhibition at 5 μl.

TABLE 2

% Inhib.
K_i

Code
Structure
at 5 μM
(μM)

T34

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84
3.7

T41

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65
6.2

T26A

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92
0.38

T28A

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84
3.6

T25A

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82
3.9

T18A

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76

T07A

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74

T22A

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72

T21A

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72

T08A

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64

T14A

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56

T03A

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Data is provided below that shows that a PGT inhibitor down-regulates PG receptor cell surface expression which, in turn, down-regulates Cox-2, the enzyme synthesizing those PGs that mediate fever, pain, and inflammation.

The inventors have formulated a model for PGT regulation of PGE₂receptor (EP) expression. A diagram of the model is provided as FIG. 8. In that model, PGT regulates peri-cellular prostaglandin E₂[PGE₂] by internalizing the PGE₂, making it unavailable for binding to cell-surface EP receptors. The binding of PGE₂to the EP receptors causes internalization of the receptors, and PGT prevents this binding by internalizing PGE₂. This sensitizes the cell to subsequent PGE₂exposure, causing more extensive prostaglandin signaling. Thus, the cell with PGT (FIG. 8 right) is sensitized to PGE₂whereas the cell without PGT or with PGT inhibited (FIG. 8 left) is desensitized to PGE₂. The experiments shown in FIGS. 9 and 10 support this model.

EP₄expression in MDCK cells that constitutively express PGT was tested by determining PGE₂binding to the cells. Wild type MDCK cells and MDCK cells stably expressing PGT were transiently transfected with human cDNA of EP₄(UMR cNDA Resource Center, www.cdna.org). About 30 hours after transfection, the cells were incubated in Waymouth buffer containing 0 and 20 nM of unlabeled PGE₂, and either 4% vehicle (DMSO) or 5 μM T26A, for 10 minutes at 37° C. The cells were then washed with PBS buffer and lysed the cells with lysing buffer containing 10 mM TrisHCl, pH. 7.5, 5 mM EDTA, 100 nM okadaic acid, and 20 μM indomethacin. The cells were then harvested and homogenized using a Potter-Elvehiem homogenizer in an ice cold solution containing 10 mM Tris-HCl, pH. 7.4, 1 mM EDTA, 0.1 mM phenylmethylsulfonyl fluoride, 20 μM indomethacin, then centrifuged the homogenate at 800 g for 5 minutes. The supernatant was transferred to another tube and centrifuged at 19000 rpm for 45 minutes. The supernatant was then discarded and the pellets washed with buffer A containing 20 mM HEPES-NaOH, pH. 7.4, 1 mM EDTA, 10 mM MgCl₂. These are extracted cell membranes for the following binding assay. The membranes were incubated in Buffer A containing 5 nM of ³H PGE₂at 30° C. for 1 hour. EP4 bound to ³H-PGE₂were quantified as described by Negishi et al., 1987. Nonspecific binding was determined by using 1000-fold excess of unlabeled PGE₂in the incubation mixture. The specific binding was calculated by subtracting the nonspecific bind value from the total binding value (Nishigaki et al., 1996).

The results are provided in FIG. 9. The cluster of overlapping data points at lower right (at [PGE₂]=55 nM) indicates lack of significant PGE₂binding in the absence of EP₄transfection. The upper curves show that inducing PGT expression increases PGE₂binding to EP₄compared to WT without PGT, and that inhibition of PGT transport function with T26 abrogates this effect.

In the similar studies shown in FIG. 10, expression of PGT expression was either permitted, or is suppressed with doxycycline (Dox). The upper curves show that expressing PGT increases PGE₂binding to EP₄, whereas suppressing PGT expression (+Dox) reduces PGE₂binding down to the level of WT cells.

Of the PGT inhibitors described above, the most potent PGT inhibitor is TGBZ T26 (FIG. 11—called T26A in Table 2). The effect of T26 IV injections on systemic BP in anesthetized rats of a control strain (WKY) and the Spontaneously Hypertensive Rat (SHR) strain was evaluated. As shown in FIG. 12, T26 reduced the blood pressure of both rats.

The utility of siRNA for reducing PGT mRNA expression was also evaluated. Cells were transfected with Qiagen siRNA oligonucleotides directed against PGT or against no known gene sequence (manufacturer's control). Total RNA was extracted 24 or 48 hrs later and subjected to Quantitative (Real Time) PCR. As shown in FIG. 13, the siRNA was effective in reducing PCT mRNA expression.

Further studies evaluated the effect of PGT inhibition on Cox-2 expression. Cells were treated with siRNA to PGT as in FIG. 13 and were harvested at 48 hrs. Cox-2 in RNA was quantified by Quantitative (Real Time) PCR (left). Cox-2 function was determined by stimulating PGE₂release with the calcium ionophore A23187±the nonselective inhibitor indomethacin or the Cox-2 selective inhibitor NS 398. Cox-2 protein was determined by immunoblotting using a polyclonal antibody (Cayman Chemicak, Inc.). As shown in FIG. 14, inhibition of PGT led to suppression of Cox-2 expression in these cells.

Example 3
Physiological Studies with PGT Inhibitor T26A

The effects of T26A (see Example 2) on rat physiology was evaluated.

T26A increases endogenous PGE₂in circulating blood of rats. Either 300 μL vehicle (4% DMSO and 4% cremophor in water) or 300 μL of 1 mM T26A was injected into the jugular vein of anesthetized rats (300-325 g weight). One mL of blood was obtained from the carotid artery 10 minutes later. PGE₂in blood samples was measured using PGE₂EIA kit from Caymanchem. FIG. 15 shows the results graphically. The acute administration of T26A (i.e., by injection) caused a greater than two-fold increase in plasma PGE₂concentrations. This finding is consistent with the model advanced in Example 2 (FIG. 8). Since PGT transports PGE₂into the cell, inhibiting PGT would be expected to inhibit removal of PGE₂from blood to be internalized and degraded.

T26A reduces the degradation of exogenously-administered PGE₂. Either 300 μL vehicle (4% DMSO and 4% cremophor in water) or 300 μL of 1 mM T26A was injected into the jugular vein of anesthetized rats (300-325 g weight). Ten minutes later 100 μL of 1 ng/μL PGE₂was injected. One mL blood was withdrawn from the carotid artery at 1, 3, 5, and 10 min after PGE₂injection. PGE₂in blood samples was measured using PGE₂EIA kit from Caymanchem. FIG. 16 shows the results graphically. The acute administration of T26A inhibited PGE₂degradation. This is also consistent with the FIG. 8 model. Inhibiting PGE₂transport into the cell reduces its intracellular degradation.

Chronic oral administration of T26A increases endogenous PGE₂in whole blood. Regular water, vehicle (2% DMSO and 2% cremophor) in drinking water, or 2 mM T26A in drinking water was administered to 17 week old mice for 16 days. At the end of the experiment, we withdrew blood by cardiac puncture. PGE₂in blood samples was measured by using PGE₂EIA kit from Caymanchem. FIG. 17 shows the results graphically. Chronic oral administration increased plasma PGE₂levels four-fold. This indicates that T26A is effective when administered orally.

Chronic oral administration of T26A increases PGE₂excretion in urine. The mice described above pertaining to FIG. 17 were placed in metabolic cages. Six days after they adjusted to the caged condition (on regular diet and water) regular drinking water, vehicle (2% DMSO and 2% cremophor) in drinking water, or 2 mM T26A in drinking water was administered for 16 days. Urine was collected daily. PGE₂in urine samples was measured using PGE₂EIA kit from Caymanchem. FIG. 18 shows the results graphically. Chronic oral administration of T26A increased urinary excretion of endogenous PGE₂. This is also consistent with the FIG. 8 model. Inhibiting PGE₂transport into the cell reduces its intracellular degradation such that more is secreted without intracellular degradation.

Chronic oral administration of T26A increases bleeding time. At the end of the experiment shown in FIGS. 17-18, bleeding time was measured using the tail cut method. About 1 cm tails of restrained mice were cut off; the diameter of the cut was kept the same for all mice. The emerging blood was blotted every 15 seconds without touching the wound. Bleeding time was measured as the point at which bleeding had stopped for 1 minute. Results are shown graphically in FIG. 19. Orally administered T26A increased bleeding time. It is believed that the blockade of endothelial PCT allows intravascular accumulation of PGE2 or prostacyclin, either of which can inhibit platelet aggregation.

T26A appears to be well-tolerated in mice. Table 3 shows treatments and data collected for toxicity studies.

TABLE 3

In vivo test of acute T26A toxicity.

Body

Mouse
Weight (g)
Injection
Condition

1
23.3
100% DMSO
Rarely moved for the first three

hours.

2
25.6
25 mM T26A, 5% DMSO, 5%
More active than mice #1, #4

Cremophor in water
and #5, but moved slowly for

the first one hour. Appeared

normal after 1 hour.

3
24.7
25 mM T26A, 5% DMSO, 5%
More actively than mice #1, #4

Cremophor in water
and #5, but moved slowly for

the first one hour. Appeared

normal after 1 hour.

4
24.8
250 mM T26A in 100% DMSO
Rarely moved for the first three

hours. Appeared normal

afterwards.

5
24.0
250 mM T26A in 100% DMSO
Rarely moved for the first three

hours. Appeared normal

afterwards.

6
21.9
6 mM T26A, 3% DMSO, 3%
Appeared normal.

Cremophor in water

7
22.1
6 mM T26A, 3% DMSO, 3%
Appeared normal.

Cremophor in water

8
20.6
6 mM T26A, 3% DMSO, 3%
Appeared normal.

Cremophor in water

9
21.0
10% DMSO, 10% Cremophor in
Appeared normal.

water

10
22.2
No injection
Normal.

All mice were male. They received one intraperitoneal injection. The injected volume was 100 uL to all of them. 14 hours after injection, they were all alive, and moved and acted normally.

The nice described in FIGS. 17-19 were studied in metabolic cages. Six days after they adjusted to the caged condition on regular diet and water, regular drinking water, or vehicle (2% DMSO and 2% cremophor) in drinking water, or 2 mM T26A in drinking water was administered for 16 days. Their water and food intake, weight body and urine volume were recorded daily. Results are shown graphically in FIG. 20. Chronic oral administration of T26A did not have any observable effects on water and food intake, body weight, or daily urine volume.

FIG. 21 provides results of post-mortem studies on the mice of FIGS. 17-19 administered either regular drinking water, or vehicle (2% DMSO and 2% cremophor) in drinking water, or 2 mM T26A in drinking water for 16 days. At the end of experiments, we isolated tissues from the mice and made H&E stained slides of tissues and examined the tissues microscopically. Chronic oral administration of T26A did not cause any pathological damage to the gastrointestinal tract.

Conclusion: T26A appears to be non-toxic when administered on a chronic oral basis in doses sufficient to raise plasma and urinary PGE₂concentrations.

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In view of the above, it will be seen that the several advantages of the invention are achieved and other advantages attained.

As various changes could be made in the above methods and compositions without departing from the scope of the invention, it is intended that all matter contained in the above description and shown in the accompanying drawings shall be interpreted as illustrative and not in a limiting sense.

All references cited in this specification are hereby incorporated by reference. The discussion of the references herein is intended merely to summarize the assertions made by the authors and no admission is made that any reference constitutes prior art. Applicants reserve the right to challenge the accuracy and pertinence of the cited references.

Number	Name	Date	Kind
5792851	Schuster et al.	Aug 1998	A
20040209881	Timmer et al.	Oct 2004	A1
20040225125	Chang	Nov 2004	A1

Prostaglandin transporter inhibitors

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