Claims
- 1. An isolated polynucleotide comprising a first nucleic acid at least 95% identical to a reference nucleic acid selected from the group consisting of:
(a) a nucleic acid consisting of nucleotides 839 to 1048 of SEQ ID NO:1; (b) a nucleic acid consisting of nucleotides 419 to 1420 of SEQ ID NO:1; (c) a nucleic acid consisting of nucleotides 416 to 1420 of SEQ ID NO:1; and (d) a nucleic acid consisting of the nucleotides encoding the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 203072; wherein said first nucleic acid encodes a polypeptide that regulates Prostate-Specific Antigen (PSA) gene expression.
- 2. The isolated polynucleotide of claim 1, wherein said first nucleic acid is at least 95% identical to a reference nucleic acid consisting of nucleotides 839 to 1048 of SEQ ID NO:1.
- 3. The isolated polynucleotide of claim 2, comprising nucleotides 839 to 1048 of SEQ ID NO:1.
- 4. The isolated polynucleotide of claim 1, wherein said first nucleic acid is at least 95% identical to a reference nucleic acid consisting of nucleotides 419 to 1420 of SEQ ID NO:1.
- 5. The isolated polynucleotide of claim 4, comprising nucleotides 419 to 1420 of SEQ ID NO:1.
- 6. The isolated polynucleotide of claim 1, wherein said first nucleic acid is at least 95% identical to a reference nucleic acid consisting of nucleotides 416 to 1420 of SEQ ID NO:1.
- 7. The isolated polynucleotide of claim 1, comprising nucleotides 416 to 1420 of SEQ ID NO:1.
- 8. The isolated polynucleotide of claim 1, wherein said first nucleic acid is at least 95% identical to a reference nucleic acid consisting of the nucleotides encoding the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 203072.
- 9. The isolated polynucleotide of claim 8, comprising the nucleotides encoding the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 203072.
- 10. The isolated polynucleotide of claim 1, further comprising a nucleotide sequence heterologous to said first nucleic acid.
- 11. A method of producing a vector comprising inserting the isolated polynucleotide of claim 1 into a vector.
- 12. A vector comprising the isolated polynucleotide of claim 1.
- 13. The vector of claim 12, wherein said polynucleotide comprises a nucleotide sequence heterologous to said first nucleic acid.
- 14. A host cell comprising the isolated polynucleotide of claim 1.
- 15. The host cell of claim 14, wherein said polynucleotide comprises a nucleotide sequence heterologous to said first nucleic acid.
- 16. An isolated polynucleotide comprising a nucleic acid encoding a first amino acid sequence at least 99% identical to a reference amino acid sequence selected from the group consisting of:
(a) amino acids 142 to 211 of SEQ ID NO:2; (b) amino acids 2 to 335 of SEQ ID NO:2; (c) amino acids 1 to 335 of SEQ ID NO:2; and (d) the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 203072; wherein said first amino acid sequence regulates Prostate-Specific Antigen (PSA) gene expression.
- 17. The isolated polynucleotide of claim 16, wherein said first amino acid sequence is at least 99% identical to amino acids 142 to 211 of SEQ ID NO:2.
- 18. The isolated polynucleotide of claim 17, comprising a nucleic acid encoding amino acids 142 to 211 of SEQ ID NO:2.
- 19. The isolated polynucleotide of claim 16, wherein said first amino acid sequence is at least 99% identical to amino acids 2 to 335 of SEQ ID NO:2.
- 20. The isolated polynucleotide of claim 19, comprising a nucleic acid encoding amino acids 2 to 335 of SEQ ID NO:2.
- 21. The isolated polynucleotide of claim 16, wherein said first amino acid sequence is at least 99% identical to amino acids 1 to 335 of SEQ ID NO:2.
- 22. The isolated polynucleotide of claim 21, comprising a nucleic acid encoding amino acids 1 to 335 of SEQ ID NO:2.
- 23. The isolated polynucleotide of claim 16, wherein said first amino acid sequence is at least 99% identical to the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 203072.
- 24. The isolated polynucleotide of claim 23, comprising a nucleic acid encoding the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 203072.
- 25. The isolated polynucleotide of claim 16, further comprising a nucleotide sequence heterologous to said nucleic acid.
- 26. A method of producing a vector comprising inserting the isolated polynucleotide of claim 16 into a vector.
- 27. A vector comprising the isolated polynucleotide of claim 16.
- 28. The vector of claim 27, wherein said polynucleotide comprises a nucleotide sequence heterologous to said nucleic acid.
- 29. A host cell comprising the isolated polynucleotide of claim 16.
- 30. The host cell of claim 29, wherein said polynucleotide comprises a nucleotide sequence heterologous to said nucleic acid.
- 31. A method of producing a polypeptide comprising culturing the host cell of claim 29 under conditions such that a polypeptide is expressed, and recovering said polypeptide.
- 32. An isolated polynucleotide comprising a nucleic acid encoding a fragment of SEQ ID NO:2 or a fragment of the amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 203072; wherein said fragment regulates Prostate-Specific Antigen (PSA) gene expression; or the complement of said nucleic acid.
- 33. The isolated polynucleotide of claim 32, further comprising a nucleotide sequence heterologous to said nucleic acid.
- 34. A method of producing a vector comprising inserting the isolated polynucleotide of claim 32 into a vector.
- 35. A vector comprising the isolated polynucleotide of claim 32.
- 36. The vector of claim 35, wherein said polynucleotide comprises a nucleotide sequence heterologous to said nucleic acid.
- 37. A host cell comprising the isolated polynucleotide of claim 32.
- 38. The host cell of claim 37, wherein said polynucleotide comprises a nucleotide sequence heterologous to said nucleic acid.
- 39. A method of producing a polypeptide comprising culturing the host cell of claim 37 under conditions such that a polypeptide is expressed, and recovering said polypeptide.
- 40. An isolated polynucleotide comprising a nucleic acid selected from the group consisting of:
(a) a nucleic acid encoding amino acids 21 to 29 of SEQ ID NO:2; (b) a nucleic acid encoding amino acids 38 to 46 of SEQ ID NO:2; (c) a nucleic acid encoding amino acids 46 to 54 of SEQ ID NO:2; (d) a nucleic acid encoding amino acids 66 to 74 of SEQ ID NO:2; (e) a nucleic acid encoding amino acids 75 to 83 of SEQ ID NO:2; (f) a nucleic acid encoding amino acids 84 to 92 of SEQ ID NO:2; (g) a nucleic acid encoding amino acids 130 to 138 of SEQ ID NO:2; (h) a nucleic acid encoding amino acids 146 to 154 of SEQ ID NO:2; (i) a nucleic acid encoding amino acids 165 to 173 of SEQ ID NO:2; (j) a nucleic acid encoding amino acids 178 to 186 of SEQ ID NO:2; (k) a nucleic acid encoding amino acids 192 to 200 of SEQ ID NO:2; (l) a nucleic acid encoding amino acids 215 to 222 of SEQ ID NO:2; (m) a nucleic acid encoding amino acids 229 to 237 of SEQ ID NO:2; and (n) a nucleic acid encoding amino acids 234 to 242 of SEQ ID NO:2.
- 41. The isolated polynucleotide of claim 40, wherein said nucleic acid is (a).
- 42. The isolated polynucleotide of claim 40, wherein said nucleic acid is (b).
- 43. The isolated polynucleotide of claim 40, wherein said nucleic acid is (c).
- 44. The isolated polynucleotide of claim 40, wherein said nucleic acid is (d).
- 45. The isolated polynucleotide of claim 40, wherein said nucleic acid is (e).
- 46. The isolated polynucleotide of claim 40, wherein said nucleic acid is (f).
- 47. The isolated polynucleotide of claim 40, wherein said nucleic acid is (g).
- 48. The isolated polynucleotide of claim 40, wherein said nucleic acid is (h).
- 49. The isolated polynucleotide of claim 40, wherein said nucleic acid is (i).
- 50. The isolated polynucleotide of claim 40, wherein said nucleic acid is (j).
- 51. The isolated polynucleotide of claim 40, wherein said nucleic acid is (k).
- 52. The isolated polynucleotide of claim 40, wherein said nucleic acid is (l).
- 53. The isolated polynucleotide of claim 40, wherein said nucleic acid is (m).
- 54. The isolated polynucleotide of claim 40, wherein said nucleic acid is (n).
- 55. An isolated polynucleotide comprising a nucleic acid encoding at least 100 contiguous amino acids of SEQ ID NO:2.
- 56. The isolated polynucleotide of claim 55, comprising a nucleic acid encoding at least 150 contiguous amino acids of SEQ ID NO:2.
- 57. The isolated polynucleotide of claim 55, further comprising a nucleotide sequence heterologous to said nucleic acid.
- 58. A method of producing a vector comprising inserting the isolated polynucleotide of claim 55 into a vector.
- 59. A vector comprising the isolated polynucleotide of claim 55.
- 60. The vector of claim 59, wherein said polynucleotide comprises a nucleotide sequence heterologous to said nucleic acid.
- 61. A host cell comprising the isolated polynucleotide of claim 55.
- 62. The host cell of claim 61, wherein said polynucleotide comprises a nucleotide sequence heterologous to said nucleic acid.
- 63. A method of producing a polypeptide comprising culturing the host cell of claim 61 under conditions such that a polypeptide is expressed, and recovering said polypeptide.
- 64. A composition comprising the isolated polynucleotide of claim 55.
- 65. An isolated polynucleotide comprising a first nucleic acid which hybridizes in a wash solution consisting of 0.1×SSC at 65° C. over the entire length of the first nucleic acid to a second nucleic acid selected from the group consisting of:
(a) a nucleic acid consisting of the coding region of the cDNA clone contained in ATCC Deposit No. 203072 or the complement thereof; and (b) a nucleic acid consisting of the coding region of SEQ ID NO:1 or the complement thereof; wherein said first nucleic acid is 600 or more nucleotides long.
- 66. The isolated polynucleotide of claim 65, further comprising a nucleotide sequence heterologous to said first nucleic acid.
- 67. A method of producing a vector comprising inserting the isolated polynucleotide of claim 65 into a vector.
- 68. A vector comprising the isolated polynucleotide of claim 65.
- 69. The vector of claim 68, wherein said polynucleotide comprises a nucleotide sequence heterologous to said first nucleic acid.
- 70. A host cell comprising the isolated polynucleotide of claim 65.
- 71. The host cell of claim 70, wherein said polynucleotide comprises a nucleotide sequence heterologous to said first nucleic acid.
- 72. An isolated polynucleotide comprising a nucleic acid encoding an amino acid sequence from position m to position n of SEQ ID NO:2, wherein m is an integer from 2 to 236, n is an integer from 243 to 335; and m is less than n.
- 73. The isolated polynucleotide of claim 72, wherein said nucleic acid encodes an amino acid sequence at least 20 amino acids in length.
- 74. The isolated polynucleotide of claim 72, further comprising a nucleotide sequence heterologous to said nucleic acid.
- 75. A method of producing a vector comprising inserting the isolated polynucleotide of claim 72 into a vector.
- 76. A vector comprising the isolated polynucleotide of claim 72.
- 77. The vector of claim 76, wherein said polynucleotide comprises a nucleotide sequence heterologous to said first nucleic acid.
- 78. A host cell comprising the isolated polynucleotide of claim 72.
- 79. The host cell of claim 78, wherein said polynucleotide comprises a nucleotide sequence heterologous to said first nucleic acid.
- 80. A method of producing a polypeptide comprising culturing the host cell of claim 78 under conditions such that a polypeptide is expressed, and recovering said polypeptide.
- 81. A polynucleotide comprising a nucleic acid fused in frame to a nucleotide sequence heterologous to SEQ ID NO:1, wherein said heterologous nucleotide sequence encodes a heterologous polypeptide, and wherein said nucleic acid is selected from the group consisting of:
(a) a nucleic acid encoding amino acids 279 to 287 of SEQ ID NO:2; (b) a nucleic acid encoding amino acids 292 to 300 of SEQ ID NO:2; (c) a nucleic acid encoding amino acids 317 to 325 of SEQ ID NO:2; (d) a nucleic acid encoding amino acids 239 to 247 of SEQ ID NO:2; (e) a nucleic acid encoding amino acids 272 to 280 of SEQ ID NO:2; and (f) a nucleic acid encoding amino acids 248 to 331 of SEQ ID NO:2.
- 82. The isolated polynucleotide of claim 81, wherein said nucleic acid encodes amino acids 292 to 300 of SEQ ID NO:2.
- 83. The isolated polynucleotide of claim 81, wherein said nucleic acid encodes amino acids 317 to 325 of SEQ ID NO:2.
- 84. The isolated polynucleotide of claim 81, wherein said nucleic acid encodes amino acids 239 to 247 of SEQ ID NO:2
- 85. The isolated polynucleotide of claim 81, wherein said nucleic acid encodes amino acids 272 to 280 of SEQ ID NO:2.
- 86. The isolated polynucleotide of claim 81, wherein said nucleic acid encodes amino acids 248 to 331 of SEQ ID NO:2.
- 87. A method of producing a vector comprising inserting the isolated polynucleotide of claim 81 into a vector.
- 88. A vector comprising the isolated polynucleotide of claim 81.
- 89. The vector of claim 88, wherein said polynucleotide comprises a nucleotide sequence heterologous to said nucleic acid.
- 90. A host cell comprising the isolated polynucleotide of claim 81.
- 91. The host cell of claim 81, wherein said polynucleotide comprises a nucleotide sequence heterologous to said nucleic acid.
- 92. A method of producing a polypeptide comprising culturing the host cell of claim 81 under conditions such that a polypeptide is expressed, and recovering said polypeptide.
- 93. An isolated polynucleotide comprising a nucleic acid encoding at least 60 contiguous amino acids of SEQ ID NO:2;
- 94. The isolated polynucleotide of claim 93, wherein said nucleic acid encodes at least 70 contiguous amino acids of SEQ ID NO:2.
- 95. The isolated polynucleotide of claim 94, wherein said nucleic acid encodes at least 80 contiguous amino acids of SEQ ID NO:2.
- 96. A method of producing a vector comprising inserting the isolated polynucleotide of claim 93 into a vector.
- 97. A vector comprising the isolated polynucleotide of claim 93.
- 98. A host cell comprising the isolated polynucleotide of claim 93.
- 99. A method of producing the polypeptide encoded by the polynucleotide of claim 93, comprising culturing a host cell comprising said polynucleotide under conditions such that said polypeptide is expressed, and recovering said polypeptide.
- 100. A polynucleotide comprising a nucleic acid fused to a nucleotide sequence heterologous to SEQ ID NO:1, wherein said heterologous nucleotide sequence encodes a heterologous polypeptide, and wherein said nucleic acid is selected from the group consisting of:
(a) a nucleic acid encoding amino acids 279 to 287 of SEQ ID NO:2; (b) a nucleic acid encoding amino acids 292 to 300 of SEQ ID NO:2; (c) a nucleic acid encoding amino acids 317 to 325 of SEQ ID NO:2; (d) a nucleic acid encoding amino acids 239 to 247 of SEQ ID NO:2; (e) a nucleic acid encoding amino acids 272 to 280 of SEQ ID NO:2; and (f) a nucleic acid encoding amino acids 248 to 331 of SEQ ID NO:2; wherein said nucleic acid is operatively associated with a promoter to express said amino acids.
- 101. The polynucleotide of claim 100, wherein said nucleic acid encodes amino acids 279 to 287 of SEQ ID NO:2.
- 102. The polynucleotide of claim 100, wherein said nucleic acid encodes amino acids 292 to 300 of SEQ ID NO:2.
- 103. The polynucleotide of claim 100, wherein said nucleic acid encodes amino acids 317 to 325 of SEQ ID NO:2.
- 104. The polynucleotide of claim 100, wherein said nucleic acid encodes amino acids 239 to 247 of SEQ ID NO:2.
- 105. The polynucleotide of claim 100, wherein said nucleic acid encodes amino acids 272 to 280 of SEQ ID NO:2.
- 106. The polynucleotide of claim 100, wherein said nucleic acid encodes amino acids 248 to 331 of SEQ ID NO:2.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of U.S. application Ser. No. 09/126,945, filed Jul. 31, 1998, which is hereby incorporated by reference.
Continuations (1)
|
Number |
Date |
Country |
Parent |
09126945 |
Jul 1998 |
US |
Child |
10838371 |
May 2004 |
US |