Claims
- 1. A method for identifying a compound that inhibits growth of a mammalian cell, the method comprising the steps of:
(a) culturing a cell in the presence or absence of the compound; (b) assaying the cell for expression or activity of one or a plurality of the genes set forth in Table 3; and (c) identifying the compound when expression or activity of at least one of the genes set forth in Table 3 is lower in the presence of the compound than in the absence of the compound.
- 2. A method according to claim 1, wherein the cell is a tumor cell.
- 3. A method according to claim 2, wherein the cell is a human tumor cell.
- 4. A method according to claim 1, further comprising the step of comparing cell growth in the presence of the compound with cell growth in the absence of the compound.
- 5. The method of claim 1, where expression of the cellular gene of Table 3 is detected by hybridization to a complementary nucleic acid.
- 6. The method of claim 1, wherein expression of the cellular gene of Table 3 is detected using an immunological reagent.
- 7. The method of claim 1, wherein expression of the cellular gene of Table 3 is detected by assaying for an activity of the cellular gene product.
- 8. A method according to claim 1, wherein expression of a cellular gene in Table 3 is assayed using a recombinant mammalian cell comprising a reporter gene operably linked to a promoter from a cellular gene in Table 3 and detecting decreased expression of the reporter gene in the presence of the compound than in the absence of the compound.
- 9. The method of claim 8, further comprising the step of assaying cell growth in the presence and absence of the compound and identifying compounds that inhibit cell growth and a gene identified in Table 3.
- 10. A method according to claim 9, wherein the cell is a tumor cell.
- 11. A method according to claim 10, wherein the cell is a human tumor cell.
- 12. The method of claim 9, where expression of the cellular gene of Table 3 is detected by hybridization to a complementary nucleic acid.
- 13. The method of claim 9, wherein expression of the cellular gene of Table 3 is detected using an immunological reagent.
- 14. The method of claim 9, wherein expression of the cellular gene of Table 3 is detected by assaying for an activity of the cellular gene product.
- 15. A compound that inhibits tumor cell growth identified according to a method of claim 1, 4, 8 or 9 and wherein the compound is not an inhibitor of RNA synthesis or protein synthesis.
- 16. A target gene for identifying compounds that inhibit tumor cell growth, wherein the target gene is a gene identified in Table 3 and inhibition of expression of the gene or activity of the gene product inhibits growth of a tumor cell.
- 17. A method for inhibiting tumor cell growth, the method comprising the steps of contacting a tumor cell with an effective amount of a compound that inhibits expression of a gene in Table 3.
- 18. A method for inhibiting tumor cell growth, the method comprising the steps of contacting a tumor cell with an effective amount of a compound that inhibits expression of a gene in Table 3, wherein the compound is identified according to the method of claims 1, 4, 8 or 9.
- 19. A method for assessing efficacy of a treatment of a disease or condition relating to abnormal cell proliferation or tumor cell growth, the method comprising the steps of:
(a) obtaining a biological sample comprising cells from an animal having a disease or condition relating to abnormal cell proliferation or tumor cell growth before treatment and after treatment with a compound according to claim 15;(b) comparing expression of at least one gene in Table 3 after treatment with a compound according to claim 15 with expression of said genes before treatment with a compound according to claim 15; and (c) determining that said treatment with a compound according to claim 15 has efficacy for treating the disease or condition relating to abnormal cell proliferation or tumor cell growth if expression of at least one gene in Table 3 is lower after treatment than before treatment.
- 20. A method for treating a disease or condition relating to abnormal cell proliferation or tumor cell growth, the method comprising the steps of administering to an animal having said disease or condition a therapeutically effective amount of a compound produced according to the method of claim 1, 4, 8 or 9 that inhibits expression of a gene in Table 3.
- 21. A compound that inhibits in a mammalian cell expression or activity of a gene identified in Table 3 wherein the compound is identified according to a method of claim 1, 4, 8 or 9 and wherein the compound is not an inhibitor of RNA synthesis or protein synthesis.
- 22. A peptide having an amino acid sequence of any one of the sequences identified by SEQ ID NOS. 229-314.
- 23. A peptidomimetic, organomimetic or chemical mimetic of a peptide according to claim 22, wherein said mimetic has substantially the same tumor cell growth inhibiting activity as the peptide for which it is a mimetic.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PCT/US02/06254 |
Feb 2002 |
WO |
|
Parent Case Info
[0001] This application claims priority to U.S. Provisional Application Serial No.: 60/306,730, filed Jul. 20, 2001.
Government Interests
[0002] This application was supported by a grant from the National Institutes of Health, No. R01 CA62099. The government may have certain rights in this invention.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60306730 |
Jul 2001 |
US |