Claims
- 1. A nucleic acid sequence encoding C-proteinase.
- 2. The nucleic acid sequence of claim 1 wherein said sequence is set forth at FIG. 6.
- 3. The nucleic acid sequence of claim 1 wherein said sequence is set forth at FIG. 7.
- 4. A nucleic acid sequence encoding C-proteinase comprising:
(a) the nucleic acid sequence of FIG. 6 or FIG. 8; (b) the nucleic acid sequence which can hybridize to the nucleic acid sequence of FIG. 6 or FIG. 8 under highly stringent hybridization conditions; or (c) the nucleic acid sequence which is a degenerate sequence of the sequences of step (a) or (b).
- 5. A polypeptide encoded by the sequence of claim 1.
- 6. A polypeptide of claim 5 encoded by the amino acid sequence of FIG. 6.
- 7. A polypeptide of claim 5 encoded by the amino acid sequence of FIG. 7.
- 8. An antifibrotic compound having the ability to bind to the protein of claim 5 for the purpose of interrupting said polypeptide's interaction with collagen.
- 9. A method for treating disorders or diseases related to fibrosis comprising administration of a pharmaceutically effective amount of the compound of claim 8 to a patient in need.
- 10. A method for treating disorders or diseases related to fibrosis comprising administration of a pharmaceutically effective amount of C-proteinase.
- 11. A method for using the DNA sequence of claim 1 to identify compounds useful in modulating the activity of C-proteinase.
- 12. A process for producing recombinant C-proteinase comprising:
(a) culturing a cell in a nutrient medium under conditions suitable for the expression of human C-proteinase, wherein said cell is transformed with an expression vector comprised of a promoter sequence operably linked to a nucleotide sequence encoding human C-proteinase; and (b) isolating the human C-proteinase.
- 13. A process for producing recombinant C-proteinase comprising:
(a) culturing a cell in a nutrient medium under conditions suitable for the expression of human C-proteinase, wherein said cell is transformed with an expression vector comprised of a promoter sequence operably linked to the nucleotide sequence of claim 1; and (b) isolating the human C-proteinase.
- 14. A process for producing recombinant C-proteinase comprising:
(a) culturing a cell in a nutrient medium under conditions suitable for the expression of human C-proteinase, wherein said cell is transformed with an expression vector comprised of a promoter sequence operably linked to the nucleotide sequence of claim 4; and (b) isolating the human C-proteinase.
1. STATEMENT OF RELATED CASE
[0001] This application is a continuation-in-part application of provisional application, U.S. Ser. No. 60/002038, filed Aug. 8, 1995.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60002038 |
Aug 1995 |
US |
Divisions (2)
|
Number |
Date |
Country |
Parent |
08872757 |
Jun 1997 |
US |
Child |
09850048 |
May 2001 |
US |
Parent |
08609187 |
Mar 1996 |
US |
Child |
08872757 |
Jun 1997 |
US |