Claims
- 1. A recombinant mammalian cell having an adenoviral E1 protein, said recombinant mammalian cell comprising:
a recombinant nucleic acid in a functional format for expressing at least one variable domain of an immunoglobulin or a functional derivative, homologue and/or fragment of said variable domain.
- 2. The recombinant mammalian cell of claim 1 further comprising a nucleic acid encoding an E1 protein.
- 3. The recombinant mammalian cell of claim 2 wherein the E1 protein is selected from the group consisting of adenoviral E1A protein, adenoviral E1B protein, or both adenoviral E1A and E1B protein.
- 4. The recombinant mammalian cell of claim 3, wherein said adenoviral E1 protein comprises an E1A protein.
- 5. The recombinant mammalian cell of claim 3, wherein said adenoviral E1 protein is an E1B protein.
- 6. The recombinant mammalian cell of claim 3, wherein said adenoviral E1 protein further comprises an E1B protein.
- 7. A recombinant mammalian cell immortalized by the presence of an adenoviral protein, said recombinant mammalian cell comprising:
a first nucleic acid in a functional format for expressing at least one variable domain of an immunoglobulin, and a nucleic acid derived from an adenovirus encoding an E1A protein.
- 8. A recombinant mammalian cell immortalized by the presence of an adenoviral protein, said recombinant mammalian cell comprising:
a nucleic acid molecule or nucleic acid molecules in a functional format for expressing heavy and light chains of an immunoglobulin, and a nucleic acid derived from an adenovirus encoding an E1 protein.
- 9. A process of producing an immunoglobulin comprising:
culturing the recombinant mammalian cell of claim 8, and expressing said immunoglobulin.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional of application Ser. No. 09/549,463, filed Apr. 14, 2002, pending, the contents of the entirety of which, including its sequence listing, is incorporated by this reference, which application claims priority under 35 U.S.C. § 119(e) to Provisional Patent Application Serial No. 60/129,452 filed Apr. 15, 1999.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60129452 |
Apr 1999 |
US |
Divisions (1)
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Number |
Date |
Country |
Parent |
09549463 |
Apr 2000 |
US |
Child |
10234007 |
Sep 2002 |
US |