Claims
- 1. A hybrid protein comprising a covalent fusion of a recombinant Coleoptera luciferase produced by expressing in a procaryotic or eucaryotic cell, a lysate of said cell, or a cell-free protein translation system a recombinant DNA encoding Coleoptera luciferase and a polypeptide having a second biological function, such that said hybrid protein both catalyzes the oxidation of luciferin to yield light and expresses said second biological function.
- 2. A hybrid protein as in claim 1 wherein said second biological function comprises having a binding site for cytoplasmic or plasma membrane structures.
- 3. A hybrid protein as in claim 1 wherein said second biological function comprises encoding an antibody binding site for Protein A.
- 4. A hybrid protein as in claim 1 wherein said second biological function comprises antigenic activity.
- 5. A hybrid protein as in claim 1 in mutated form comprising a covalent fusion of a mutated recombinant Coleoptera luciferase produced by expressing in a procaryotic or eucaryotic cell, a lysate of said cell, or a cell-free protein translation system a recombinant DNA encoding Coleoptera luciferase and a polypeptide having a second biological function, such that said hybrid protein both catalyzes the oxidation of luciferin to yield light and expresses said second biological function.
- 6. A hybrid protein as in claim 5 comprising recombinant Coleoptera luciferase in mutated form which exhibits altered light emission properties.
- 7. A hybrid protein as in claim 6 wherein said altered light emission property comprises a wavelength different from that of the light yielded by the hybrid protein comprising the unmutated form of recombinant Coleoptera luciferase.
- 8. A hybrid protein as in claim 6 wherein said altered light emission property comprises a duration of light emission different from that of the hybrid protein comprising the unmutated form of recombinant Coleoptera luciferase.
- 9. A recombinant Coleoptera luciferase produced by expressing in a procaryotic or eucaryotic cell, a lysate of said cell, or a cell-free protein translation system a recombinant DNA encoding Coleoptera luciferase, wherein said recombinant Coleoptera luciferase catalyzes the oxidation of luciferin to yield light.
- 10. A recombinant Coleoptera luciferase as in claim 9 wherein said recombinant DNA encodes Coleoptera luciferase selected from the families Lampyridae and Elateridae.
- 11. A recombinant Coleoptera luciferase as in claim 10 wherein said recombinant DNA encodes Coleoptera luciferase from the genus Photinus.
- 12. A recombinant Coleoptera luciferase as in claim 9 in mutated form produced by expressing in a procaryotic or eucaryotic cell, a lysate of said cell, or a cell-free protein translation system a mutated recombinant DNA encoding Coleoptera luciferase, wherein said recombinant Coleoptera luciferase catalyzes the oxidation of luciferin to yield light.
- 13. A recombinant Coleoptera luciferase in mutated form as in claim 12 wherein said mutated recombinant DNA is mutated from a recombinant DNA which encodes Coleoptera luciferase selected from the families Lampyridae and Elateridae.
- 14. A recombinant Coleoptera luciferase in mutated form as in claim 13 wherein said mutated recombinant DNA is mutated from a recombinant DNA which encodes Coleoptera luciferase from the genus Photinus.
- 15. A recombinant Coleoptera luciferase in mutated form as in claim 12 which exhibits altered light emission properties.
- 16. A recombinant Coleoptera luciferase in mutated form as in claim 15 wherein said altered light emission property comprises a wavelength different from that of the light yielded by the unmutated form of recombinant DNA.
- 17. A recombinant Coleoptera luciferase in mutated form as in claim 15 wherein said altered light emission property comprises a duration of light emission different from that of the unmutated form of recombinant DNA.
- 18. A recombinant Coleoptera luciferase produced by expressing in a procaryotic or eucaryotic cell, a lysate of said cell, or a cell-free protein translation system a recombinant RNA encoding Coleoptera luciferase, wherein said recombinant Coleoptera luciferase catalyzes the oxidation of luciferin to yield light.
- 19. A recombinant Coleoptera luciferase as in claim 18 wherein said recombinant RNA encodes Coleoptera luciferase selected from the families Lampyridae and Elateridae.
- 20. A recombinant Coleoptera luciferase as in claim 19 wherein said recombinant RNA encodes Coleoptera luciferase from the genus Photinus.
- 21. A recombinant Coleoptera luciferase as in claim 18 in mutated form produced by expressing in a procaryotic or eucaryotic cell, a lysate of said cell, or a cell-free protein translation system a mutated recombinant RNA encoding Coleoptera luciferase, wherein said recombinant Coleoptera luciferase catalyzes the oxidation of luciferin to yield light.
- 22. A recombinant Coleoptera luciferase in mutated form as in claim 21 wherein said mutated recombinant RNA is mutated from a recombinant RNA which encodes Coleoptera luciferase selected from the families Lampyridae and Elateridae.
- 23. A recombinant Coleoptera luciferase in mutated form as in claim 22 wherein said mutated recombinant RNA is mutated from a recombinant RNA which encodes Coleoptera luciferase from the genus Photinus.
- 24. A recombinant Coleoptera luciferase in mutated form as in claim 18 which exhibits altered light emission properties.
- 25. A recombinant Coleoptera luciferase in mutated form as in claim 24 wherein said altered light emission property comprises a wavelength different from that of the light yielded by the unmutated form of recombinant RNA.
- 26. A recombinant Coleoptera luciferase in mutated form as in claim 24 wherein said altered light emission property comprises a duration of light emission different from that of the unmutated form of recombinant RNA.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a divisional of application Ser. No. 08/060,091, filed May 10, 1993, now U.S. Pat. No. 5,583,024, which is a continuation of application Ser. No. 07/792,644, filed Nov. 15, 1991, now abandoned, which in turn is a continuation of application Ser. No. 07/119,096, filed Nov. 10, 1987, now abandoned, which in turn is a continuation-in-part of application Ser. No. 06/803,820, filed Dec. 2, 1985, now abandoned.
Government Interests
This invention was made with Government support under Grant No. PCM 8305446 with the National Science Foundation and Grant No. AI-07194 with the United States Department of Health and Human Services and the University of California. The Government has certain rights in this invention.
Non-Patent Literature Citations (2)
Entry |
Wood J. Bioluminescence and Chemiluminescence, 5:107-114 (1990). |
Gruber, "Investigations on redevelopment of multiplexed living cell biosensors using beetle luciterase", Masters Thesis, Univ. of Wisconson-Madison, 1993. |
Divisions (1)
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Number |
Date |
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Parent |
60091 |
May 1993 |
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Continuations (2)
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Number |
Date |
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Parent |
792644 |
Nov 1991 |
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Parent |
119096 |
Nov 1987 |
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Continuation in Parts (1)
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Date |
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803820 |
Dec 1985 |
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