Claims
- 1. A process of inhibiting inflammation in an individual in need of such treatment comprising administering to the individual an effective amount of a peptide NADPH oxidase inhibitor, wherein the inhibitor inhibits ADPH oxidase activity in a cell-free assay containing (i) neutrophil cell cytosol, (ii) deoxycholate-treated neutrophil cell membranes and (iii) a Rac protein.
- 2. The method of claim 1, wherein said inhibitor exhibits GAP activity.
- 3. The method of claim 1, wherein said polypeptide or protein is selected from the group consisting of p190 GAP, CDC42Hs GAP, and polypeptide derivatives thereof.
- 4. The method of claim 1, wherein said inhibitor is a derivative of Rac.
- 5. The method of claim 1, wherein said peptide comprises mastoparan or a derivative thereof.
- 6. The method of claim 1, wherein said peptide is selected from the group of amino acid residue sequences represented by the formulae:GNIFANLFKGLFGKKE (SEQ ID NO 7); GLTISSLFSRLFGKKQ (SEQ ID NO 8); TISSLFSRLFGKKQ (SEQ ID NO 9); SSLFSRLFGKKQ (SEQ ID NO 10); CPPPVKKRKRK (SEQ ID NO 11); CPQPTRQQKRA (SEQ ID NO 12); PGKARKKSS (SEQ ID NO 13); EKKKPKKKS (SEQ ID NO 14); and VSALGIDFKVKTIYRN (SEQ ID NO 15).
- 7. The method of claim 1, wherein said peptide is selected from the group of amino acid residue sequences represented by the formulae:INLKALAALAKKIL-NH2 (SEQ ID NO 1); INLKALAALAKALL-NH2 (SEQ ID NO 2); LKALAALAKKIL-NH2 (SEQ ID NO 3); INWKGIAAMAKKLL-NH2 (SEQ ID NO 4); and VDWKKIGQHILSVL-NH2 (SEQ ID NO 5).
- 8. The method of claim 1 wherein the Rac protein is Rac2.
CROSS REFERENCE TO RELATED APPLICATION
This is a divisional of U.S. patent application Ser. No. 08/156,552, filed Nov. 15, 1993, now U.S. Pat. No. 5,726,155, which is a continuation-in-part of U.S. patent application Ser. No. 08/102,944, filed Aug. 2, 1993, now abandoned, the disclosures of which are hereby incorporated by reference.
Government Interests
This invention was supported in part by National Institutes of Health Contract HL 48008. The government has certain rights in the invention.
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Date |
Kind |
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Sep 1993 |
|
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Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
08/102944 |
Aug 1993 |
US |
Child |
08/156552 |
|
US |