Claims
- 1. A method to remove ras-mediated neoplastic cells from a cellular composition suspected of containing such neoplastic cells, which method comprises contacting the cellular composition with reovirus under conditions which results in oncolysis of the ras-mediated neoplastic cells.
- 2. The method of claim 1 wherein the cellular composition comprises hematopoietic stem cells.
- 3. The method of claim 2 wherein the hematopoietic stem cells have been harvested from bone marrow.
- 4. The method of claim 2 wherein the hematopoietic stem cells have been harvested from blood.
- 5. The method of claim 1 wherein the cellular composition comprises a tissue, an organ or any portion of a tissue or an organ.
- 6. The method of claim 5 wherein the tissue or organ is selected from the group consisting of liver, kidney, heart, cornea, skin, lung, pancreatic islet cells, and whole blood.
- 7. The method of claim 5 wherein the tissue, organ or portion of the tissue or organ is useful for transplantation.
- 8. The method of claim 1 wherein the cellular composition comprises cultured cells, semen or eggs.
- 9. The method of claim 1 wherein the reovirus is a mammalian reovirus.
- 10. The method of claim 1 wherein the reovirus is an avian reovirus.
- 11. The method of claim 9 wherein the mammalian reovirus is a human reovirus.
- 12. The method of claim 9 wherein the mammalian reovirus is serotype 3 virus.
- 13. The method of claim 12 wherein the serotype 3 virus is the Dearing strain.
- 14. The method of claim 1 further comprising the step of freezing and storing the reovirus-treated composition in a solution containing DMSO.
- 15. The method of claim 1 further comprising the step of treating the reovirus-treated composition with anti-reovirus antibodies and complements.
- 16. The method of claim 1 further comprising the steps of subjecting the treated cells to a gradient which separates the cells from the reovirus and collecting the layer which contains cells.
- 17. A method of preparing a cellular composition for transplantation into a recipient, comprising the steps of:
(a) selecting a cellular composition for transplantation; and (b) contacting the composition with a reovirus under conditions which result in oncolysis of ras-mediated neoplastic cells.
- 18. The method of claim 17 wherein said transplantation is autologous.
- 19. The method of claim 17, further comprising the step of administering to the transplant recipient at least one substance selected from the group consisting of an anti-reovirus antibody and/or an immune system stimulating agent.
- 20. A method of reducing a risk of recurrence of tumor due to transplantation of autologous hematopoietic stem cell suspected of containing ras-mediated neoplastic cells, comprising harvesting from a subject to receive the transplant a cellular composition which comprises hematopoietic stem cells, contacting the cellular composition with a reovirus under conditions which result in oncolysis of ras-mediated neoplastic cells, and introducing the reovirus-treated composition back into the subject.
- 21. A cellular composition prepared according to the method of claim 1.
- 22. The composition of claim 21 which is used in transplantation.
- 23. The composition of claim 22 wherein said transplantation is autologous.
- 24. The composition of claim 23 wherein said composition comprises hematopoietic stem cells.
RELATED INVENTIONS
[0001] This application claims the benefit of U.S. Provisional Application Ser. No. 60/201,990, filed May 3, 2000, Ser. No. 60/205,389, filed May 19, 2000 and Ser. No. 60/268,054, filed Feb. 13, 2001, under 35 U.S.C. §119(e). The entire disclosure of each of the above provisional applications is hereby incorporated by reference.
Provisional Applications (3)
|
Number |
Date |
Country |
|
60201990 |
May 2000 |
US |
|
60205389 |
May 2000 |
US |
|
60268054 |
Feb 2001 |
US |