Claims
- 1. A method for normalizing the scattered intensities of light from particles suspended in a solvent and illuminated by a collimated light beam comprising the steps of
- A. Measuring the intensity of light transmitted undeviated through the sample and external to the sample containing cell, I.sub.s ;
- B. Measuring a fraction of the incident light source intensity before entering the cell, I.sub.f ;
- C. Dividing all measured scattering intensities at specified scattering angles by the quantity .sqroot.I.sub.f I.sub.s, where I.sub.s is the intensity value measured in step A and I.sub.f is the intensity value measured in step B.
- 2. The method of claim 1 where the sample is confined to a channel and the illuminating light beam has a diameter much smaller than the diameter of the channel.
- 3. The method of claim 1 where the light beam is from a laser.
- 4. The method of claim 3 where the light from the laser is polarized.
- 5. The method of claim 1 where the fraction of the incident light source intensity is obtained from a beam splitter, said beam splitter dividing the incident beam into a source beam which illuminates the sample and a reference beam which illuminates the source beam monitor.
- 6. The method of claim 1 where the sample is contained in a cell between two transport windows and said illuminating collimated light beam passes through first transparent window means, then through said sample, and finally through second transparent window means.
- 7. The method of claim 6 where the medium external to each said transparent window means is air.
- 8. The method of claim 1 where the collimated light beam is plane polarized.
- 9. A sample cell monitor for generating a normalization factor by which means scattering light intensity values measured at a plurality of angles may be normalized by dividing each measured intensity by said normalizing factor, said sample cell containing a suspension of particles in a solvent solution that is illuminated by a collimated light beam that passes undeviated through the sample cell by entrance and exit window means provided therefore, and comprised of
- A. Detector means for measuring a value proportional to the incident light beam intensity before said beam enters said sample cell;
- B. Detector means for measuring a value proportional to the intensity of the light beam passing undeviated through the sample cell;
- C. Multiplication means forming a value proportional to the product of the value from said incident light beam detector means and said undeviated light beam detector means;
- D. Normalization generation means that forms normalization factor proportional to the square root of the product value of step C;
- E. Division means to divide said scattered light intensity values by said normalization factor.
- 10. The monitor of claim 9 where the collimated light beam is a laser.
- 11. The monitor of claim 10 where the light from the laser is polarized.
- 12. The monitor of claim 9 where the incident light beam is split by a beam splitter before the said light beam enters the sample cell and where said split component is measured by said detector means of step A.
Parent Case Info
This is a continuation of application Ser. No. 861,863, filed May 12, 1986, now abandoned, which is a division of application Ser. No. 671,181, filed Nov. 15, 1985, now U.S. Pat. No. 4,616,927.
Government Interests
Some of the developments and studies associated with this invention were performed under Contract #DAMD17-84-C-4155 from the U.S. Army Medical Research and Development Command. The Government has certain non-exclusive rights under the terms of this contract.
US Referenced Citations (5)
Non-Patent Literature Citations (1)
Entry |
Arkin et al., Statistical Methods, Barnes & Noble, Inc., New York, fifth edition, copyright 1970, pp. 29-30. |
Divisions (1)
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Number |
Date |
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671181 |
Nov 1985 |
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Continuations (1)
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Number |
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861863 |
May 1986 |
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