Claims
- 1-19. (canceled)
- 20. An uncleared cell culture comprising:
(a) a population of replicable genetic packages, each of which displays on its surface a fusion protein that comprises a surface-displayed replicable genetic package polypeptide and an exogenous polypeptide; (b) a complex that comprises a target molecule and one or more members of the population of replicable genetic packages that specifically bind to said target molecule via said exogenous polypeptide; and (c) cells in which the replicable genetic packages were amplified prior to contact with the target molecule.
- 21. The uncleared cell culture of claim 20, wherein said replicable genetic packages are selected from the group consisting of bacteriophage and eukaryotic viruses.
- 22. The uncleared cell culture of claim 20, wherein said target molecule is immobilized on a solid support.
- 23. The uncleared cell culture of claim 22, wherein said solid support is selected from the group consisting of: a bead, a chip, a microtiter plate, a prokaryotic cell and a eukaryotic cell.
- 24. The uncleared cell culture of claim 20, wherein said target molecule is selected from the group consisting of: a polypeptide, a nucleic acid, an RNA, a DNA, a small organic molecule, and a carbohydrate.
- 25. The uncleared cell culture of claim 20, wherein said exogenous polypeptide is an antibody.
- 26. The uncleared cell culture of claim 25, wherein said antibody is a scFv or a Fab.
- 27. The uncleared cell culture of claim 20, wherein the uncleared cell culture further comprises a detection reagent that specifically binds to the replicable genetic packages.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PCT/US01/18421 |
Jun 2001 |
WO |
|
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Patent application Ser. No. 60/209503, filed on Jun. 5, 2000, the teachings of which are herein incorporated by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60209503 |
Jun 2000 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09874547 |
Jun 2001 |
US |
Child |
10727745 |
Dec 2003 |
US |