Claims
- 1. A method for screening a mixture of compounds for activity comprising steps of:
a. contacting the mixture with a system which mimics an organ capable of metabolizing the mixture in an appropriate time to generate metabolites; and b. determining the activity of the generated metabolites.
- 2. A method for quantitating a mixture of compounds comprising steps of:
a. contacting the mixture with a system which mimics an organ capable of metabolizing the mixture in an appropriate time to generate metabolites; and b. determining the activity of the generated metabolites.
- 3. A method for identification of an active metabolite of a mixture of compounds comprising steps of:
a. contacting the mixture with a system which mimics an organ capable of metabolizing the mixture in an appropriate time to generate metabolites; and b. determining the activity of the generated metabolites.
- 4. The method of claim 1, 2 or 3, wherein the organ is a liver.
- 5. The method of claim 4, wherein the system is human liver microsomes.
- 6. A method for screening a mixture of compounds for activity comprising steps of:
a. administering the mixture to a subject capable of metabolizing the mixture; and b. taking bodily fluid which contains metabolites of the mixture from the subject, to determine the activity of the metabolite.
- 7. A method for quantitating an herbal extract comprising steps of:
a. administering the mixture to a subject capable of metabolizing the mixture; and b. taking bodily fluid which contains metabolites of the mixture from the subject, to determine the activity of the metabolite.
- 8. A method for identification of the active metabolite of a mixture of compounds comprising steps of:
a. administering the mixture to a subject capable of metabolizing the mixture; and b. taking bodily fluid which contains metabolites of the mixture from the subject, to determine the activity of the metabolite.
- 9. The method of claim 1, 2, 3, 6, 7, or 8, wherein the activity of the metabolites is determined by in vitro assay.
- 10. The method of claim 9, wherein the assay is determined by the inhibition of cell growth.
- 11. The method of claim 10, wherein the cells are cancerous cells.
- 12. The method of claim 1, 2, 3, 6, 7, or 8, wherein the activity of the metabolites is determined by inhibition of cyclin B1 activity.
- 13. The method of claim 12, wherein the inhibition is at least 50%.
- 14. The method of claim 1, 2, 3, 6, 7, or 8, wherein the mixture is from a natural product.
- 15. The method of claim 14, wherein the natural product is an herb.
- 16. The active metabolite identified by the method of claim 3 or 8.
- 17. A pharmaceutical composition comprising an effective amount of the metabolite of claim 16 and a pharmaceutically acceptable carrier.
- 18. A method of producing a fingerprint of an extract of a natural product comprising steps of:
a. contacting the extract with a system which mimics an organ capable of metabolizing the extract in an appropriate time to generate metabolites; and b. determining the identity and amount of the metabolites generated, thereby generating a fingerprint of the extract.
- 19. The method of claim 18, wherein the organ is a liver.
- 20. The method of claim 19, wherein the system is human liver microsomes.
- 21. A method of producing a fingerprint of an extract of a natural product comprising steps of:
a. administering the extract to a subject capable of metabolizing the extract; and b. determining the metabolites generated, thereby generalizing a fingerprint of the extract.
- 22. The method of claim 13 or 16, wherein the natural product is an herb.
- 23. The fingerprint produced by claim 18 or 21.
- 24. A method to determine the batch-to-batch variation of an extract from a natural product comprising comparison of the fingerprint of claim 23 of different batches.
- 25. A method to assay for formulation variation of an extract from a natural product comprising comparison of the fingerprint of claim 23 of different batches.
- 26. A method to assay for dose variation of an extract from a natural product comprising comparison of the fingerprint of claim 23 of different batches.
- 27. A method for identification of induced compounds in a subject comprising steps of:
a. administering a mixture of compounds to the subject; and b. extracting bodily fluid from the subject to determine the generation of induced compound; and c. identifying said induced compound.
- 28. The method of claim 27, wherein the mixture is an extract from a natural product.
- 29. The induced compounds identified by claim 27.
- 30. The method of claim 6, 7, 8, 21, or 27, wherein the subject is a human.
- 31. A method for treating cancer in a subject comprising administering to the subject an effective amount of coptis chinesis extract.
- 32. The method of claim 31, wherein the cancer is a solid tumor.
- 33. A method for treating cancer in a subject comprising administering to the subject an effective amount of coptis chinesis extract and a therapeutic agent.
- 34. The method of claim 33, further comprising a protein kinase C inhibitor.
- 35. The method of claim 33, wherein the therapeutic agent is a microtubule-destabilizing agent.
- 36. The method of claim 33, wherein the treating of the coptis chinesis extract and the therapeutic agent is performed in a sequential manner.
- 37. The method of claim 36, wherein the subject is treated with coptis chinesis extract first, then a therapeutic agent.
- 38. The method of claim 37, wherein the therapeutic agent is a microtubule-destabilizing agent.
- 39. The method of claim 35 or 38, wherein the microtubule-destabilizing agent is a taxol or taxol-like compound.
- 40. An anti-tumor composition comprising an effective amount of coptis chinesis extract.
Parent Case Info
[0001] This application claims priority of U.S. Ser. No. 60/421,411, filed 25 Oct. 2002, the content of which is incorporated by reference here into this application.
Government Interests
[0002] The invention disclosed herein was made with United States Government support under U.S. Army DOD Breast Cancer Grant No. DAMD17-02-0486 from the United States Department of Department of Defense. Accordingly, the United States Government has certain rights in this invention.
Provisional Applications (1)
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Number |
Date |
Country |
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60421411 |
Oct 2002 |
US |