Claims
- 1. A crosslinked protein composition, said protein prior to crosslinking comprising at least 70 weight % of repetitive units of GAGAGS (SEQ ID NO:01) and GVGVP (SEQ ID NO:02), where in at least two units an amino acid is substituted with at least one of lysine or arginine, said protein having a lysine and arginine equivalent weight in the range of 1 to 20 kD, and said protein comprising at least two amino acids which comprise a functional group capable of reacting with a crosslinking agent.
- 2. A method of sealing a defect in viable tissue, said method comprising:injecting into said defect a precursor composition comprising a polyfunctional crosslinking agent and a protein polymer comprising at least 40 weight % of repetitive units of from 3 to 15 amino acids of at least one naturally occurring structural protein, where said protein polymer comprises at least two amino acids which comprise a functional group capable of reacting with said crosslinking agent, whereby said precursor composition sets up to a strongly adherent adhesive composition; whereby said defect is sealed.
- 3. A method according to claim 2, wherein said units are selected from the group consisting of GAGAGS (SEQ ID NO:01), GVGVP (SEQ ID NO:02) and GXX, wherein the X's are the same or different and X is any amino acid, at least 10 number % and not more than 60 number % of X's being proline.
- 4. A method according to claim 2, wherein said units are GAGAGS (SEQ ID NO:01) and GVGVP (SEQ ID NO:02) and are present to form a block copolymer protein.
- 5. A method according to claim 2, wherein said tissue is the wall of a vessel selected from an artery, vein, capillary, lung, dura or colon.
- 6. A method according to claim 2 wherein said injection is done to augment tissue mass.
- 7. A method of filling a defect in viable tissue, said method comprising:injecting into said defect a precursor composition comprising a polyfunctional crosslinking agent and a protein polymer comprising at least 40 weight % of repetitive units of from 3 to 15 amino acids of at least one naturally occurring structural protein, where said protein polymer comprises at least two amino acids which comprise a functional group capable of reacting with said crosslinking agent, whereby said precursor composition sets up to a strongly adherent adhesive composition; and whereby said defect is filled.
- 8. A method according to claim 7 wherein said injection is done to augment tissue mass.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of application Ser. No. 08/642,246, filed May 2, 1996, now U.S. Pat. No. 6,033,654, which is a continuation-in-part of application Ser. No. 08/435,641, filed May 5, 1995, now U.S. Pat. No. 5,817,303, whose disclosure is incorporated herein by reference.
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Foreign Referenced Citations (4)
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Country |
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Nov 1992 |
JP |
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WO |
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Continuations (1)
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Number |
Date |
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Parent |
08/642246 |
May 1996 |
US |
Child |
09/451206 |
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US |
Continuation in Parts (1)
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Number |
Date |
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Parent |
08/435641 |
May 1995 |
US |
Child |
08/642246 |
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US |