Claims
- 1. A method for constructing a solid immersion lens structure, the method comprising:
producing a mold so as to define a pliant lens cavity in which a spherical solid immersion lens portion of the solid immersion lens structure is to be formed, the pliant lens cavity having an orifice having a transverse dimension less than a transverse dimension of the lens cavity for the spherical solid immersion lens portion; casting a moldable material, from which at least the solid immersion lens portion is to be formed, into at least the lens cavity; permitting the moldable material to set thereby to form the solid immersion lens portion of the solid immersion lens structure; and removing the solid immersion lens portion of the solid immersion lens structure from the pliant mold after the moldable material has set.
- 2. A method according to claim 1, wherein producing the mold comprises forming the mold from a thermally-resilient deformable material.
- 3. A method according to claim 2, wherein forming the mold comprises casting a first catalyzed crosslinkable liquid around a mold core in the form of a generally spherical element until it is set as the mold, removing the mold core from the mold after the moldable material has set wherein a region of the mold adjacent the orifice is allowed to temporarily deform thereby to permit the spherical element to be removed with minimal damage to the mold, casting a second catalyzed crosslinkable liquid into the mold until it has set as moldable material, and removing the solid immersion lens portion from the mold after the moldable material has set wherein the region of the mold adjacent the orifice is allowed to temporarily deform thereby to permit the solid immersion lens portion to be removed.
- 4. A method according to claim 3, wherein forming the mold comprises casting moldable material into the container to form a first layer from which the mold is to be formed and permitting the first layer to set, positioning the mold core in the container and casting the moldable material into the container at least partially to encapsulate the mold core, then positioning the mold core on the first layer, and then casting the moldable material into the container to at least partially encapsulate the mold core, thereby to form a second layer immediately adjacent the first layer.
- 5. A method according to claim 4 wherein the generally spherical mold core protrudes from the upper surface of the first layer.
- 6. A method according to claim 5 further comprising casting the moldable material from which at least the solid immersion lens portion of the solid immersion lens structure is to be formed into the container to fill not only the lens cavity, but also to form a layer in the container, the layer defining an upper surface above the lens cavity, such that the layer forms a body portion of the solid immersion lens structure when the moldable material has set, the body portion then being integrally molded together with the solid immersion lens portion.
- 7. A method according to claim 6 further comprising coating the lens cavity and the upper surface of the mold with a release agent to inhibit adhesion of the solid immersion lens portion and the body portion of the solid immersion lens structure to the mold at the upper surface and in the lens cavity.
- 8. A method according to claim 6 further comprising forming a channel adjacent the layer defining the upper surface above the lens cavity before the moldable material has set.
- 9. A method according to claim 1 wherein the moldable material from which at least the first layer is formed comprises a silicone elastomer.
- 10. A method according to claim 9 wherein the moldable material from which at least the lens portion is formed comprises a silicone elastomer.
- 11. A method for imaging an object, the method comprising:
guiding the object along a passage defined by an integrally molded together solid immersion lens structure, the solid immersion lens structure defining a body portion, in which the passage is defined, and a solid immersion lens portion, the solid immersion lens portion being optically aligned with a position in the passage; positioning the object in the passage at the position which is optically aligned with the solid immersion lens portion so that the object is within a field of view extending through the solid immersion lens portion; and viewing the object in the passage through the solid immersion lens portion while the object is at the position in the passage which is optically aligned with the solid immersion lens portion.
- 12. A method as claimed in claim 11, wherein the solid immersion lens structure defines an inlet, leading into the passage, and an outlet, leading from the passage, guiding the object along the passage comprising passing the object through the inlet and along the passage.
- 13. A method as claimed in claim 12, wherein the object is supported in a liquid, passing the object through the inlet and along the passage comprising pumping the liquid along the passage.
- 14. A method as claimed in any one of claims 13, wherein viewing the object through the solid immersion lens while at the position in the passage which is optically aligned with the solid immersion lens portion comprises optically aligning a microscope with the solid immersion lens portion, and viewing the object through the microscope.
- 15. A solid immersion lens structure comprising:
a solid immersion lens portion; and a body portion from which the solid immersion lens portion extends, at least the solid immersion lens portion being of a molded material.
- 16. A solid immersion lens structure as claimed in claim 15, wherein the body portion is also of a molded material.
- 17. A solid immersion lens structure as claimed in claim 16, wherein the solid immersion lens portion and the body portion are of an integrally molded together material.
- 18. A solid immersion lens structure as claimed in claim 17; wherein the solid immersion lens portion and the body portion are of an integrally molded together material comprising a silicone elastomer.
- 19. A solid immersion lens structure as claimed in claim 18, wherein the solid immersion lens portion is frusto-spherical in shape.
- 20. A solid immersion lens structure as claimed in claim 15, wherein the body portion defines a passage extending therethrough, the passage defining a position in optical alignment with the solid immersion lens portion.
- 21. An imaging system comprising:
a microscope arrangement; and a solid immersion lens structure as claimed in any one of claims 15 to 20.
- 22. An imaging system as claimed in claim 21, wherein the microscope arrangement comprises a laser microscope.
- 23. A photon collection system comprising:
a photon counter; optics for directing emitted photons to said photon counter; and a solid immersion lens structure as claimed in any one of claims 15 to 20.
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT
[0001] The US Government has certain rights in this invention pursuant to Grant No. HG 01642 awarded by the National Institute of Health and under Contract No. PHY-9722417 of the National Science Foundation.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09640907 |
Aug 2000 |
US |
Child |
09927290 |
Aug 2001 |
US |