Claims
- 1. A substantially purified Fas.DELTA.TM polypeptide which has an amino acid sequence corresponding to the sequence of a human Fas antigen which is capable of signaling in response to a ligand therefor, wherein the Fas.DELTA.TM polypeptide comprises the intracellular and extracellular domains of said Fas antigen but lacks a portion of the transmembrane domain, and wherein the expression of a nucleic acid encoding said polypeptide in mammalian cell affords a Fas.DELTA.TM polypeptide as a soluble, secreted protein.
- 2. A Fas.DELTA.TM polypeptide according to claim 1, wherein the polypeptide is produced by a process comprising the steps of culturing a mammalian cell containing a nucleic acid encoding the polypeptide under conditions suitable for the expression thereof, whereby the Fas.DELTA.TM polypeptide is secreted from the cell; and purifying said Fas.DELTA.TM polypeptide.
- 3. A Fas.DELTA.TM polypeptide according to claim 2, wherein said nucleic acid is heterologous to said mammalian cell.
- 4. A Fas.DELTA.TM polypeptide according to claim 1, wherein said polypeptide is a native protein.
- 5. A composition comprising a substantially purified Fas.DELTA.TM polypeptide according to claim 1 and a physiologically acceptable buffer.
- 6. A substantially pitied protein comprising the amino acid sequence of SEQ ID NO:19.
Parent Case Info
This application is a division of allowed application Ser. No. 08/152,443, filed Nov. 15, 1993.
Foreign Referenced Citations (1)
Number |
Date |
Country |
0510691 |
Apr 1992 |
EPX |
Divisions (1)
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Number |
Date |
Country |
Parent |
152443 |
Nov 1993 |
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