Claims
- 1. An isolated polynucleotide comprising a member selected from the group consisting of:(a) a polynucleotide which encodes a polypeptide comprising amino acids 1 to 174 of SEQ ID NO:2; and (b) a polynucleotide which is fully complementary to the polynucleotide of (a).
- 2. An isolated polynucleotide comprising the entire Staphylococcus aureus spsA coding sequence that is SEQ ID NO:1 wherein the insolated polynucleotide is not genomic DNA.
- 3. An isolated polynucleotide comprising a polynucleotide sequence which encodes a polypeptide comprising amino acids 1 to 174 of SEQ ID NO:2 wherein the insolated polynucleotide is not genomic DNA.
- 4. An isolated polynucleotide comprising a member selected from the group consisting of:(a) a polynucleotide which encodes the same mature polypeptide expressed by the DNA contained in NCIMB Deposit No. 40771 and having the polynucleotide sequence of SEQ ID NO:1; and (b) a polynucleotide that is fully complementary to the polynucleotide of (a).
- 5. An immunological composition comprising an isolated DNA which encodes the Stapyhlococcus aureus spsA polypeptide having the amino acid sequence of SEQ ID NO:2, which, when introduced into a mammal, induces an immunological response in the mammal to said polypeptide.
- 6. An expression system comprising the polynucleotide of claim 3 that produces a polypeptide comprising the amino acid sequence of SEQ ID NO:2 when the expression system is present in a compatible host cell.
- 7. A process for producing a recombinant host cell comprising transforming or transfecting into the cell the expression system of claim 6 such that the host cell, under appropriate culture conditions, produces a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
- 8. A recombinant host cell produced by the process of claim 7.
- 9. A process for producing a polypeptide comprising culturing the recombinant host cell of claim 8 under conditions sufficient for the production of the polypeptide and recovering the polypeptide from the culture.
- 10. An isolated polynucleotide consisting of a polynucleotide encoding the polypeptide sequence set forth in SEQ ID NO:2.
RELATED APPLICATIONS
This application claims benefit of U.S. patent application Ser. No. 60/027,218, filed Sep. 30, 1996, U.S. patent application Ser. No. 60/027,220, filed Oct. 1, 1996, and U.S. patent application Ser. No. 60/027, 075, filed Sep. 30, 1996.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5798211 |
Ohno et al. |
Aug 1998 |
|
Foreign Referenced Citations (3)
Number |
Date |
Country |
0 841 394 A2 |
May 1998 |
EP |
WO 9401583A |
Jan 1994 |
WO |
WO 9814461 A1 |
Apr 1998 |
WO |
Non-Patent Literature Citations (2)
Entry |
Cregg, et al., “Molecular Cloning and Expressionof the spsB Gene Encoding an Essential Type I Signal Peptidase from Staphylococcus aureus”, Journal of Bacteriology, 178(19), pp. 5712-5718, (1996). |
Georgiou, “Optimizing the Production of Recombinant Proteins in Microorganisms”, AIChE Journal, 34(8), pp. 1233-1248, (1988). |
Provisional Applications (3)
|
Number |
Date |
Country |
|
60/027218 |
Sep 1996 |
US |
|
60/027220 |
Oct 1996 |
US |
|
60/027075 |
Sep 1996 |
US |