Claims
- 1. A method for stabilizing a composition comprising an airborne virus comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I
- 2. The method of claim 1 wherein said airborne virus is an Adenovirus.
- 3. The method of claim 2 wherein the Adenovirus is a recombinant Adenovirus expressing a transgene.
- 4. The method of claim 1, wherein the non-ionic detergent is a Brij detergent, a polysorbate (Tween) detergent, a pluronic molecule, or a Triton-like molecule.
- 5. The method of claim 1, wherein the concentration ranges from about 0.005% to about 0.1% (vol/vol).
- 6. The method of claim 5, wherein the concentration ranges from about 0.05% to about 0.08% (vol/vol).
- 7. A method for stabilizing a composition comprising an airborne virus comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent which comprises an alkyl moiety and a polyethylene glycol moiety.
- 8. The method of claim 7 wherein said airborne virus is an Adenovirus.
- 9. The method of claim 7, wherein said non-ionic detergent has the structure shown in Formula I
- 10. The method of claim 9, wherein R is substituted by one or more of carboxy, carbamide, halogen, hydroxy, amino, or 1-3 rings, which can be aromatic or cycloalkyl or can also be heterocyclic.
- 11. The method of claim 10, wherein said 1-3 rings are substituted by one or more of alkyl, hydroxy, amino, halogen, nitro, sulfoxy, carboxy or carbamide.
- 12. The method of claim 9 wherein,
in Formula I, R is (CH3)(CH2)Y—, wherein Y is 10-15; in Formula II, R is CaH(2a+1)CO2—, wherein a is 10 to 70; in Formula III, R is CH3, X is 55,Y is 29 and Z=55,or R is CH3,X is 98,Y is 67 and Z is 98; or in Formula IV, R′ is (CH3)3C.CH2C(CH3)2—, A is phenylene, and X is 9-10, or R′ is (CH3)3C.CH2C(CH3)2—, A is cyclohexylene, and X is 9-10.
- 13. The method of claim 12 wherein in Formula I R is (CH3)(CH2)Y—, X is 23 and Y is 12 or R is (CH3)(CH2)Y—, X is 20 and Y is 12.
- 14. The method of claim 12 wherein in Formula II R is C11H23CO2—.
- 15. A method for stabilizing a composition comprising an Adenovirus comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent which comprises an alkyl moiety and a polyethylene glycol (PEG) moiety.
- 16. A method for stabilizing a composition comprising an Adenovirus, comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I
- 17. The method of claim 16, wherein said alkyl is substituted by one or more of carboxy, carbamide, halogen, hydroxy or amine, or by 1-3 rings, which can be aromatic or cycloalkyl or can also be heterocyclic.
- 18. The method of claim 17, wherein said 1-3 rings can be substituted by one or more of alkyl, hydroxy, amine, halogen, nitro, sulfoxy, carboxy or carbamide.
- 19. A method for stabilizing a composition comprising Adenovirus, comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I
- 20. A method to stabilize a composition comprising Adenovirus, comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I
- 21. A method for stabilizing a composition comprising an airborne virus, comprising adding to the composition a stabilizing-effective amount of a non-Ionic detergent of Formula I
- 22. The method of claim 21, wherein said alkyl is substituted by one or more of carboxy, carbamide, halogen, hydroxy or amine, or by 1-3 rings, which can be aromatic or cycloalkyl or can also be heterocyclic.
- 23. The method of claim 22, wherein said 1-3 rings can be substituted by one or more of alkyl, hydroxy, amine, halogen, nitro, sulfoxy, carboxy or carbamide.
- 24. A method for stabilizing a composition comprising an airborne virus, comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I.
- 25. A method to stabilize a composition comprising airborne virus, comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I
- 26. A method for stabilizing a composition comprising an airborne virus to reduce loss of virus amount or activity comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I
- 27. The method of claim 26 wherein said loss is less than about 10%
- 28. The method of claim 27 wherein said loss is less than about 5%.
- 29. The method of claim 28 wherein said loss is less than about 2%.
- 30. A pharmaceutical composition comprising an airborne virus a stabilizing-effective amount of a non-ionic detergent of Formula I,
- 31. The composition of claim 30, wherein said airborne virus is an Adenovirus.
- 32. The composition of claim 30, wherein the non-ionic detergent is a Brij detergent, a polysorbate (Tween) detergent, a pluronic molecule, or a Triton-like molecule.
- 33. The composition of claim 30, wherein, the non-ionic detergent is present at a concentration ranging from about 0.005% to about 0.1% (vol/vol).
- 34. The composition of claim 33, wherein the concentration ranges from about 0.05% to about 0.08% (vol/vol).
- 35. The composition of claim 34, wherein the concentration is about 0.05% (vol/vol).
- 36. A pharmaceutical composition comprising an Adenovirus, Tween 20 at a concentration of about 0.05% (vol/vol), and a pharmaceutically acceptable carrier comprising 2 mM MgCl2 , 2% sucrose (wt/vol) and water.
- 37. A pharmaceutical composition, comprising
a) an Adenovirus, b) a stabilizing-effective amount of a non-ionic detergent of Formula I R—O(CH2CH2O)X—H I wherein
X is 4-30, and R is a linear or branched alkyl of 10-70 carbon atoms, or Formula II 44 wherein R is —CO2R′ having 10 to 70 carbons, and W+X+Y+Z=20, wherein R′ is as above for R, or Formula III 45 wherein R is as above, and X is 5-100, Y is 25-75 and Z is 50-100, or Formula IV 46 wherein X is 5-15 ring A is phenylene or cyclohexylene, and R′ is R as above, or a combination thereof, and c) at least one pharmaceutically acceptable carrier.
- 38. The composition of claim 37, wherein the Adenovirus is a recombinant Adenovirus.
- 39. The composition of claim 37, wherein the Adenovirus is a recombinant Adenovirus suitable for gene therapy.
- 40. The composition of claim 37, wherein the detergent is present at a concentration ranging from 0.005% to 0.1% (vol/vol).
- 41. The method of claim 15, wherein said detergent is present at a concentration ranging from 0.005% to 0.1% (vol/vol).
- 42. The method of claim 15, wherein the Adenovirus is a recombinant Adenovirus.
- 43. The method of claim 42, wherein the recombinant Adenovirus is suitable for gene therapy.
- 44. A pharmaceutical composition, comprising a stabilized Adenovirus composition made by the method of claim 1 and at least one pharmaceutically acceptable carrier.
- 45. A pharmaceutical composition, comprising a stabilized airborne virus composition made by the method of claim 1 and at least one pharmaceutically acceptable carrier.
- 46. The method of claim 25, wherein the detergent is polysorbate 20 (Tween 20).
- 47. The method of claim 7, wherein the detergent is in a concentration of 0.005% to 0.1% (vol/vol).
- 48. A pharmaceutical composition, comprising
a) an airborne virus, b) a stabilizing-effective amount of a non-ionic detergent of according to Formula I R—O(CH2CH2O)X—H I wherein
X is 4-30, and R is a linear or branched alkyl of 10-70 carbon atoms, or Formula II 47 wherein R is —CO2R′ having 10 to 70 carbons, and W+X+Y+Z=20, wherein R′ is as above for R, or Formula III 48 wherein R is as above, and X is 5-100, Y is 25-75 and Z is 50-100, or Formula IV 49 wherein X is 5-15 ring A is phenylene or cyclohexylene, and R′ is R as above, or a combination thereof; and c) at least one pharmaceutically acceptable carrier.
- 49. The composition of claim 48, wherein the detergent is in a concentration of 0.005% to 0.1% (vol/vol).
- 50. The method of claim 1, wherein said alkyl is substituted by one or more of carboxy, carbamide, halogen, hydroxy or amine, or by 1-3 rings, which can be aromatic or cycloalkyl or can also be heterocyclic.
- 51. The method of claim 49, wherein said 1-3 rings can be substituted by one or more of alkyl, hydroxy, amine, halogen, nitro, sulfoxy, carboxy or carbamide.
Parent Case Info
[0001] This application claims the benefit of the filing date of U.S. Provisional Application Serial No. 60/349,222 filed Jan. 18, 2002.
Provisional Applications (1)
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Number |
Date |
Country |
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60349222 |
Jan 2002 |
US |