Claims
- 1. An isolated polynucleotide comprising the nucleic acid sequence of ORF ID NO: 2, represented by nucleotides 164-1405 of SEQ ID NO:1.
- 2. The isolated polynucleotide of claim 1, wherein said polynucleotide comprises a heterologous polynucleotide sequence.
- 3. The isolated polynucleotide of claim 2, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide.
- 4. A method for making a recombinant vector comprising inserting the isolated polynucleotide of claim 1 into a vector.
- 5. A nucleic acid sequence complimentary to the polynucleotide of claim 1.
- 6. A recombinant vector comprising the isolated polynucleotide of claim 1.
- 7. The recombinant vector of claim 6, wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene expression.
- 8. A recombinant host cell comprising the isolated polynucleotide of claim 1.
- 9. The recombinant host cell of claim 8, wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene expression.
- 10. An isolated polynucleotide for the detection of Staphylococcus aureus, wherein said isolated polynucleotide consists of at least 800 contiguous nucleotides of the nucleic acid sequence of ORF ID NO: 2, represented by nucleotides 164-1405 of SEQ ID NO:1.
- 11. The isolated polynucleotide of claim 10, wherein said polynucleotide comprises a heterologous polynucleotide sequence.
- 12. The isolated polynucleotide of claim 11, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide.
- 13. A method for making a recombinant vector comprising inserting the isolated polynucleotide of claim 10 into a vector.
- 14. A nucleic acid sequence complimentary to the polynucleotide of claim 10.
- 15. A recombinant vector comprising the isolated polynucleotide of claim 10.
- 16. The recombinant vector of claim 15, wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene expression.
- 17. A recombinant host cell comprising the isolated polynucleotide of claim 10.
- 18. The recombinant host cell of claim 17, wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene expression.
- 19. The isolated polynucleotide of claim 10, wherein said isolated polynucleotide consists of at least 850 contiguous nucleotides of the nucleic acid sequence of ORF ID NO: 2, represented by nucleotides 164-1405 of SEQ ID NO:1.
- 20. The isolated polynucleotide of claim 10, wherein said isolated polynucleotide consists of at least 900 contiguous nucleotides of the nucleic acid sequence of ORF ID NO: 2, represented by nucleotides 164-1405 of SEQ ID NO:1.
- 21. The isolated polynucleotide of claim 10, wherein said isolated polynucleotide consists of at least 1000 contiguous nucleotides of the nucleic acid sequence of ORF ID NO: 2, represented by nucleotides 164-1405 of SEQ ID NO:1.
Parent Case Info
This application is the National Stage of International Application No. PCT/US99/06199, filed Mar. 18, 1999, which claims benefit under 35 U.S.C. § 119(e) of U.S. Provisional Application Nos. 60/078,682, filed Mar. 20, 1998, 60/080,296, filed Apr. 1, 1998, and 60/084,674, filed May 7, 1998.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/US99/06199 |
|
WO |
00 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO99/47662 |
9/23/1999 |
WO |
A |
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
5302527 |
Birkett et al. |
Apr 1994 |
A |
5587307 |
Alborn et al. |
Dec 1996 |
A |
Foreign Referenced Citations (1)
Number |
Date |
Country |
9710316 |
May 1994 |
WO |
Non-Patent Literature Citations (3)
Entry |
Alborn et al., Cloning and characterization of femA and femB from Staphylococcus epidermidis, Gene, 180:177-181 (1996). |
Sugai et al., epr, Which encodes Glycylglycine Endopeptidase Resistance, is Homologous to femAB and Affects Serine Content . . . , J. Bacteriol., 179(13):43114318 (Jul. 1997). |
Thumm et al., Studies on prolysostaphin processing and characterization of the lysostaphin immunity factor (Lif) of . . . , Mol. Microbiol., 23(6):1251-1265 (1997). |
Provisional Applications (3)
|
Number |
Date |
Country |
|
60/084674 |
May 1998 |
US |
|
60/080296 |
Apr 1998 |
US |
|
60/078682 |
Mar 1998 |
US |