Claims
- 1. A non-human transgenic animal comprising a transgene encoding a fatty acid desaturase.
- 2. The transgenic non-human animal according to claim 1, wherein said animal is a mammal.
- 3. The transgenic non-human animal according to claim 2, wherein said mammal is an ungulate.
- 4. The transgenic non-human animal according to claim 1, wherein said animal is poultry.
- 5. The transgenic non-human animal according to claim 1, wherein said transgene is chromosomally integrated.
- 6. The transgenic non-human animal according to claim 1, wherein said transgene comprises a coding sequence for a stearoyl-CoA desaturase operably linked to an animal tissue specific promoter.
- 7. The transgenic non-human animal according to claim 6, wherein said animal tissue specific promoter is a mammary specific promoter.
- 8. The transgenic non-human animal according to claim 6, wherein said animal tissue specific promoter is an intestinal epithelium specific promoter.
- 9. An expression cassette comprising a coding sequence for a stearoyl-CoA desaturase operably linked to a heterologous mammalian tissue-specific promoter.
- 10. The expression cassette according to claim 9, wherein said heterologous tissue specific promoter is a mammary specific promoter.
- 11. The expression cassette according to claim 9, wherein said heterologous tissue specific promoter is an intestinal epithelium specific promoter.
- 12. The expression cassette according to claim 9, wherein said expression cassette is present in a vector.
- 13. A method for producing a non-human transgenic animal comprising a fatty acid desaturase transgene, said method comprising:
(a) introducing a desaturase transgene into a single-celled embryo, forming a genetically modified embryo; and (b) transferring the genetically modified embryo into a recipient female of the same species as the embryo, wherein the genetically modified embryo develops into a transgenic animal in the female.
- 14. The method according to claim 13, wherein said transgenic animal is chosen from a mouse, a rat, a rabbit, a pig, a sheep, a goat, poultry, and a cow.
- 15. The method according to claim 13, wherein the transgenic animal is a mammal, and said transgene is expressed in mammary gland cells of said mammal.
- 16. The method according to claim 13, wherein the transgenic animal is a mammal, and wherein said transgene is expressed in intestinal epithelium cells of said mammal.
- 17. The method according to claim 13, wherein the desaturase transgene is a stearoyl-CoA desaturase transgene.
- 18. A method for producing a non-human transgenic animal comprising a fatty acid desaturase transgene, said method comprising:
a) introducing a desaturase transgene into a somatic cell, forming a genetically modified somatic cell comprising a genetically modified nucleus; b) transferring the genetically modified nucleus from the genetically modified somatic cell into a single-celled embryo, generating a genetically modified single-celled embryo; and c) transferring the genetically modified single-celled embryo into a recipient female of the same species as the embryo, wherein the genetically modified embryo develops into a transgenic animal in the female.
- 19. The method of claim 18, wherein the desaturase transgene is a stearoyl CoA desaturase transgene.
- 20. A method of producing a food product, said method comprising harvesting a food product from a non-human transgenic animal of claim 1.
- 21. A method of producing a food product, the method comprising processing a food product harvested from a non-human transgenic animal of claim 1.
- 22. A food product harvested from a non-human transgenic animal of claim 1.
- 23. The food product according to claim 22, wherein the food product is processed.
- 24. The food product according to claim 22, wherein said food product is milk.
- 25. The food product according to claim 22, wherein said food product is meat.
- 26. The food product according to claim 22, wherein said food product is an egg.
- 27. The food product according to claim 22, wherein the food product has from about 10 to about 67 weight percent saturated fatty acids.
- 28. The food product according to claim 22, wherein the food product has from about 27 to about 80 weight percent monounsaturated fatty acids.
- 29. The food product according to claim 22, wherein the food product has from about 7.5 to about 25 weight percent polyunsaturated fatty acids.
- 30. The food product according to claim 22, wherein the food product has from about 0.400 to about 50 weight percent conjugated linoleic acid.
CROSS-REFERENCE
[0001] This application claims the benefit of U.S. Provisional Patent Application No. 60/411,719, filed Sep. 17, 2002, which application is incorporated herein by reference in its entirety.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60411719 |
Sep 2002 |
US |