Patent Application
20220317114
The present invention relates to the analysis of filamentary structures, in particular filamentary structures such as vital tissues, natural or synthetic fibers.
More specifically, the present invention relates to the qualitative-quantitative study of the surface characteristics, mechanical properties, and, indirectly, structural properties of filaments, in particular vital or reconstituted tissues, in particular skin appendages, more particularly hair, eyelashes, or structures associated therewith.
Skin appendages are structures which have a close functional link and a common embryological origin with the skin. Amongst these, hair and eyelashes are epidermal formations placed respectively on the head and on the end of the eyelids, which, from a physiological point of view, provide a function mainly of protection.
The hair and eyelashes are constituted prevalently by keratin, a fibrous scleroprotein which is responsible for most of the mechanical properties of such structures.
The optical properties of the hair (or eyelash) in itself, and of hairstyles as a whole, depend to a large extent both on the outermost and surface part of the hair, i.e. the cuticle, and on the more or less distinctive presence of pigments (such as melanin, natural and/or synthetic pigments) situated in the various portions of the hair (cuticle, cortex and medulla). Both the mechanical and optical properties can be more or less distinctly affected and modified as a result of mechanical, physical or chemical treatments, or as a result of the application and deposition of various substances. Many of the treatments to which skin appendages are subjected are closely associated with the lifestyle of modern Western culture, the roots themselves of which lifestyle is based in an extremely old and well-established tradition. By way of example, it is sufficient to mention the processes of dyeing, and of temporary or permanent modification of the hairstyle which constitute some of the most important habits associated with modern lifestyle.
Products for the health and care of the hair which comprise, inter alia, detergents, conditioners, fixatives, protection agents, etc., together with those for the make-up and treatment of the eyelashes, are playing a strongly increasing part in the cosmetics and pharmaceuticals market.
In parallel with the growth of the market for cosmetics products, the effects claimed by such products have also increased. For this purpose, at European level, (CE) Regulation 1223/2009 has been introduced, and has been in force since July 2013. Amongst other things, this Regulation makes it compulsory to verify the truthfulness of properties claimed which are not strictly correlated with the nature itself of the cosmetic product. For this purpose, it is essential to have and validate instrumental methods which can verify these effects. In particular, in order to verify the effectiveness and performance levels of a cosmetic formulation, it is mandatory to develop adequate experimental designs, carrying out evaluations which are preferably “in vivo,” in other words in real conditions of use, which can take place for quite different periods of time, varying from a few minutes to months, in relation to the type of product and the functionalities to be ascertained.
In view of the enormous interest relating to the study of hair, to the formulative development in this context and to the corresponding evaluation of effectiveness, there are numerous approaches, and consequently numerous technologies which have been developed and adapted for the study of the surface characteristics of the hair. In the first instance, reference is made here to the surface characteristics, since these are the focus of attention of most of the products and treatments for eyelashes and hair, and consequently the properties claimed by the corresponding advertising campaigns.
In general it can be said that, in order to characterize the surface properties of the hair, recourse is usually made to two options:
The first method of approach includes a vast range of equipment, which in most cases can determine the friction which a probe of varied geometry encounters when passing through the sample. One of the most common and widely used examples of this technique is constituted by the combability test. This test consists of passing a probe, commonly shaped as a comb or brush, through a lock of hair, and measuring the friction thereof which the probe encounters. An exclusively mechanical example of this approach is found in patent U.S. Pat. No. 3,459,197 entitled “Comb-mounted hair analysis gauge” in which there is measurement of the deflection of a comb which is deformed because of the friction which it encounters during its use. This deformation is recorded on a graduated scale.
U.S. Pat. No. 4,167,869 entitled “Apparatus for measuring hair grooming force” records the same parameter, i.e. the friction encountered by a comb which is applied to a lock of hair, basing the reading of the datum on the modification of the probe resistance values associated with the comb. The variation of these resistance values is translated into an electronic signal which permits continuous recording of the friction value concerned.
Although based on the same structural plan, some technological solutions record and amplify for example the noise or the vibrations produced by the probe whilst it is being moved on the sample. Examples of such technological solutions are described in the Japanese patent application JP 2004 159830 entitled “Hair evaluation system” or in the Japanese patent application JP 2009 201812 entitled “Sensor for measuring hair characteristic and evaluation apparatus and evaluation method using the same.”
All the examples referred to hitherto can be used both on samples prepared ex vivo and on locks of hair in vivo.
However, structures exist which can be considered as accessories of common tensiometers, by means of the use of which it is possible to carry out tests ex vivo with quantification, as in some cases previously cited, of the resistance encountered by a probe substantially in the shape of a comb, whilst it is slid on a sample of hairs. The main advantage of this technique consists of the possibility of implementing a regular movement of the probe. An example of an accessory of this type is the product known as a “hair combing rig” made by the company Stable Micro System.
The second method of approach includes a series of equipment which is extremely sophisticated, and is often unable to operate on samples in vivo, which equipment further requires laborious and costly preparation of the sample in order to be able to carry out the analysis, as well as needing image capturing apparatuses which are even more sophisticated and costly. Examples of this approach are various goniophotometry techniques often associated with DSLR (Digital Single-Lens Reflex) cameras and with polarized sources of light. In some cases, analysis in vivo is possible, as for example in the system identified as Samba Hair System, produced by the US company BOSSA NOVA TECHNOLOGIES.
The subject of US 2012/320191 is a method and a device for analyzing hair fibers by means of the positioning of the fibers on an image sensor which receives light from a source and evaluates the images of the fibers by means of a processor, correlating in succession the analysis values generated by the processor with predetermined descriptors of the properties of the hair. The invention according to U.S. 2012/320191 A1 can potentially also analyze in vivo a suitably irradiated and photographed sample of hairs. However, this equipment, in addition to capturing and analyzing images generated exclusively by the radiation transmitted by the irradiated sample, is not at all suitable for the study of samples constituted by numerous elements such as the hairs of an entire lock, nor by eyelash samples. In fact, in order to be able to operate correctly, the programs which are allocated to the analysis must operate on images relating to fibers which are distinct from one another. This aspect makes the process of preparation of the sample difficult and complicated, and is not easily transferable in the hands of inexpert operators.
Equipment has also been patented which can potentially combine both the approaches. An example of a strategy of this type is described in DE 10 2004 037566 A1. This document describes equipment which is provided with an optical measuring device, and can simultaneously carry out traction tests on a sample of hairs. Leaving out of consideration the objective difficulty both of miniaturizing this invention and making it easily and effectively usable in vivo, it is also important to emphasize that, in fact, it does not lend itself to the analysis of samples comprising a series of filaments simultaneously, since it is designed mainly to carry out traction tests on samples of single skin appendages (for example single hairs).
In order to be able to use this equipment in these contexts, accessories and protocols have been developed and adapted which are suitable for the most varied requirements. However, although this equipment is extremely precise and accurate, it is characterized by some extremely significant limits. These limits include above all:
In this context, it is particularly important to be able to have user-friendly equipment which is easy to transport, has a limited cost, and can combine simplicity of use with the possibility of obtaining realistic and reproducible qualitative-quantitative data both in vitro and in vivo, also using non-specialized personnel, and in the most varied operative contexts, ranging from a beauty salon to an experimental clinical laboratory.
The objective of the present invention is to make available instrumentation suitable for fulfilling the need for qualitative-quantitative evaluation of the surface characteristics, of some mechanical properties, and, indirectly, of some structural properties of filaments, in particular of vital or reconstituted tissues, in particular of hairs and eyelashes, whether they are vital or reconstituted, or of structures associated therewith, such as, by way of non-limiting example, artificial eyelashes, or evaluation of the effect of formulations for reconstruction or extension, and/or with treatment and/or decorative functions.
A further objective of the invention is to make available instrumentation which can permit the execution of measurements for the qualitative-quantitative characterization of filaments, and more particularly hairs and eyelashes, also, but not limited to, “in vivo” with non-invasive operations, for the implementation of protocols of characterization with a variable duration and in diversified conditions.
According to the present invention, these objectives are achieved by a system for the characterization of vital or reconstituted tissues, in particular skin appendages such as hairs or eyelashes or structures associated therewith as described and claimed herein.
Particular embodiments are also described.
To summarize, the present invention is based on the principle of creating an aggregated characterization system, suitable for the study of filaments, in particular, but not limited to, hairs or eyelashes, using conservative methods associated with the acquisition of digital images which make it possible to monitor the analysis processes continually.
The operation of the characterization system which is the subject of the invention is based on the computerized analysis of digital images relating to phenomena of reflection and/or dispersion and/or fluorescence obtained by irradiating with a predetermined source of electromagnetic radiation, for example a source of light radiation, a prepared sample, positioned and optionally treated for this purpose. A prepared sample is a series of homogenous filaments per structural type, such as, by way of non-limiting example, a lock of hairs or a series of eyelashes of a person.
According to a particular embodiment, the correct positioning of the sample relative to the characterization system is obtained by using a gaseous flow, for example, but not limited to, air.
According to a further embodiment, the correct positioning of the sample relative to the characterization system is obtained thanks to dragging surfaces associated with the system.
In particular contexts, the invention in question can also constitute an accessory of equipment which is already in use, such as, but not limited to, tensiometers.
More particularly, the characterization system makes it possible to acquire images of reflection and/or dispersion generated by different sources including, but not limited to, sources of incoherent light (including with different frequencies) and/or lasers with different wavelengths and/or sources of radiation which generate fluorescence and/or sources of polarized light, etc.
This system can acquire data and digital images also of materials which cannot be removed during the stages of evaluation and study, and can therefore also operate in vivo and in situ. This advantageously permits the development of qualitative-quantitative evaluation protocols.
The process of analysis of the data and digital images acquired is carried out thanks to the use of a processor program executed by a control unit which is integrated in the system or is associated therewith, including remotely, based on the analysis of digital images acquired by means of an acquisition device created according to the teaching of the present invention.
According to the teaching of the present invention, in accordance with a particular embodiment, there may also be provided a device for tensioning of the sample being examined, comprising a roller or a similar support rotating at a defined and controllable speed which, during the movement of rotation in contact with a sample to be examined, for example in the movement of sliding on the surface of the hairs of a sample lock, encounters a friction determined by the structure and surface condition of said sample.
In these particular operative contexts and given applications, the system can be provided with supplementary structures, which will be described hereinafter, being able to evaluate, whether in vivo or not, also the forces of friction, sliding or rolling of suitably designed probes relative to filaments, to skin appendages (in particular, but not limited to, hairs and eyelashes) and other structures, which structures are also developed for the purpose of simulating biological structures, such as, for example, but not limited to, locks of hair, artificial or reconstituted eyelashes, etc.
This therefore makes available a system for analysis in continuum which does not interfere with the processes to be evaluated in progress, which system can also operate in vivo and without damaging the sample in question.
The extreme simplicity of use and ease and immediacy of reading of the data produced make the instrument also particularly suitable for, but not limited to, demonstrative use, for example in a beauty salon, not requiring for its operation particularly specialized personnel or particular environmental conditions.
Further characteristics and advantages of the present invention will be described in greater detail in the following detailed description of an embodiment thereof, provided by way of non-limiting example, with reference to the appended drawings, in which:
The system comprises:
The source of electromagnetic radiation (light and/or not light) 12 permits the generation of figures of reflection/dispersion/fluorescence by a sample S illuminated by an analysis radiation. This can include a source of incoherent light (for example, but not limited to, a white light LED source or any visible or non-visible wavelength) or a source of polarized light, or, in addition or alternatively, a source of coherent light (for example a laser), characterized by a given wavelength. These sources can optionally also have a frequency suitable for the generation of phenomena of fluorescence on the irradiated sample.
Different images of reflection/dispersion/fluorescence of the sample S can correspond to different sources of radiation.
Optionally, the intensity of the sources of radiation can be regulated, and in this case different images of reflection/dispersion/fluorescence of the sample S can correspond to different electromagnetic flows.
Different sources can also be present simultaneously, and can preferably be oriented as required relative to the sample S. Alternatively, they are present selectively, and it is possible to replace or exchange them as required for the purpose of being able to carry out the selection of the most appropriate source of radiation in the shortest possible time.
According to a preferred embodiment, the sources can advantageously be directed, focused, and their intensity can be regulated independently, so as to adopt a predetermined angle of incidence of the radiation emitted on the sample. Different images of reflection/dispersion/fluorescence can correspond to different angles of incidence.
Advantageously, at least one source of coherent light (laser) is used, with planar geometry which can irradiate the sample along a linear section thereof, preferably perpendicularly to the longitudinal axis of the fibers which constitute it. A source of this type with planar geometry can irradiate the sample with a “blade” of light. When the “blade” of light meets the sample, it is partly reflected, partly passes through the sample, and is partly dispersed both on the surface and in the interior thereof The whole of these phenomena gives rise to an image of the sample.
The image acquisition device 14 is designed to capture images generated by the irradiation of the sample by the source(s), using for example, but not exclusively, a digital camera or a digital microscope depending on the necessary level of enlargement required by the analysis. The level of enlargement can be varied as required according to the type of analysis in progress, and different digital acquisition apparatuses, including of different types, can be present simultaneously. Advantageously, according to a preferred embodiment, it is also possible to vary the point of observation of the sample by varying the position of the acquisition device 14. It is possible to capture both individual images of the sample, and films. Advantageously, the images which are captured are saved and processed directly by the system by the processing means 18, or alternatively they can be sent to external processing means.
A structure 16 for delimiting the area of acquisition of the images can be present, and, according to a less complex embodiment, comprises a support base characterized by the presence of an opening, optionally with a variable size, corresponding to the field of view of the image acquisition device. This structure can also advantageously contribute to maintaining the position of the sample during the acquisition of the images.
The processing means 18 are configured by means of a processor program which can execute a qualitative-quantitative analysis of the images and/or of the films captured. This analysis can be carried out directly, immediately after the acquisition of the images, or at any subsequent moment.
Specifically, the processor program which exists in the processing means 18 is designed to analyze digital images of filaments, for example, but not limited to, skin appendages, in particular hairs and eyelashes, irradiated with different types of optical radiation, such as, for example, but not limited to, laser radiation, and acquired by means of, for example, but not limited to, a microscope or another acquisition device.
The analysis is carried out by means of specific algorithms suitable for the qualitative-quantitative definition of parameters such as gloss, size, color, and indirectly the mechanical properties, in order to acquire information on the state of the sample, for example a lock of hair, and on the effect produced by any treatment carried out on the hair.
The analysis is carried out using known algorithms systems, such as Kayyali, Kirsch and Laplacian (3×3, 5×5 and Gaussian), examining a non-linear area created from amongst all the minimum and maximum image comparison peaks. A stage of presetting of the analysis procedure is dedicated to the acquisition of all the images, and the data acquired is used for construction of homogenous classes wherein each new sample can be classified; each of these classes can correspond to a series of parameters for the definition of analysis protocols. Thus, all the images acquired by means of the different forms of lighting, for a single type of sample, for example a lock of hair, form a preselection.
Advantageously, the processor program has a record section for storage of the record data of the people tested, so that in this section it is possible to consult and control all the analyses carried out and start a new analysis by acquiring the images necessary and selecting the preselection for the purpose of comparing the analyses.
By taking into consideration a historic development of the analysis results, it is possible to understand whether a treatment of the sample has produced an effect.
The archive of the tests carried out can be saved in order to have a historic development of the treatments and of their effects on the sample, for example hair, and to constitute a database.
The database can be supplied via the Internet to all the users so as to improve the statistical reliability of the results obtained.
The analysis of the sample, for example hairs, is carried out in an image analysis stage, by loading the images and comparing them with the corresponding reference classes previously defined, or with the images relating to the same person or sample, acquired at different times. During this stage of the program, it is possible to carry out the analysis without necessarily having to identify the person to whom the sample belongs.
Each analysis is carried out on the basis of the predefined parameters. For example, an analysis can be carried out on the basis of the percentage of similarity of the sample to the reference image, preferably using the following parameters:
In its definitive version, the processor program can be integrated directly inside a portable device, and show only the result on a display unit which is connected directly. Alternatively, the device can send the data captured wirelessly to a PC which will carry out the analysis and upkeep of the data itself.
The analysis of the sample is not necessarily based on the separation, characterization and quantification of the various fractions of radiation after the interaction with the sample. Advantageously, it is not necessary, for example, to establish and quantify the fraction of radiation which is reflected by the surface of the sample, or the fraction which passes through it, but it is the image of the sample as a whole which is informative.
The real and extreme structural simplification of most of the embodiments of the present invention is associated with a real possibility of miniaturization, a fundamental aspect which permits simple and widespread use thereof, in particular for applications in vivo, as well as a restriction of the production costs.
In different embodiments, the system has additional elements which provide further characteristic functions.
According to a second embodiment, the system according to the invention also includes a region 20 for alignment and stretching of the sample, and a grasping system (not illustrated) which is equipped with devices for command and control of the system, as shown in
The region 20 for alignment and stretching comprises for example a support 20a and a gripping frame 20b. The sample S is extended or stretched on the support 20a which permits homogenous, flat and continuous distribution thereof. In order to assist this process, if the sample is constituted by fibers of a significant length, such as, for example, but not limited to, fibers or hairs, it can be advantageous to make the region 20 for alignment and stretching, or a part thereof, slide along the sample, thus assisting the distribution and stretching thereof, including optionally integrally with the region 20.
The selection of the sample is greatly simplified since it is not necessary to select one fiber at a time, or a few fibers which are well separated and aligned with one another. On the contrary, in the most demanding hypothesis, it is sufficient to select a sample which is quantitatively sufficient to cover a defined surface, in the most stringent cases operating on the basis of a criterion of “minimum quantity” necessary to guarantee the cover of a given surface (for example in the case of analysis of locks of hair both in vivo and ex vivo) whereas in fact in other cases (such as, for example as far as the study of eyelash samples is concerned) it is sufficient to include in the field of irradiation an arbitrary number of elements of the sample in relation to which the analysis is being carried out.
The grasping system comprises the devices necessary for the operation of the system according to the present invention, including control of the region of alignment and stretching, as well as the commands relating to, for example, but not limited to, switching on/switching off of the sources of light, capture of the images/films, etc. Optionally, there can also be a display unit for direct display of the region of alignment and of the images/films acquired.
According to a third embodiment, as shown in
The sample in question can optionally be distributed prevalently in a generally planar region, so as to avoid where possible the presence of different focal planes. This objective can be achieved in various ways, some of which are cited hereinafter.
The sample in question can advantageously be supported and/or distributed on a homogenous surface which can include a flat or curved surface.
The color as well as the roughness and/or gloss of the surface of the support element is of significant importance. A non-limiting example of a structure of this type is represented in
According to the present embodiment, a process of stretching and tensioning of the sample could be advantageous. In this case, this process is carried out by a vacuum source (not illustrated) which subjects the sample to tension by suctioning it, or alternatively blowing it, in the interior of a conduit 22a with an appropriate geometry, thus giving rise to the adhesion and distribution on the analysis surface 22b of the support element 22, as shown in
The sample can be blocked by mechanical means, in particular, but not necessarily, during the “in vitro” analysis, which means secure it in an appropriate position and prevent undesirable movements thereof. These means can for example, but not necessarily, include a pair of jaws which block the sample, closing it in the form of a clamp. By way of non-limiting example, the jaws may comprise two parallelepipeds or two simple opposing rollers or cylinders. Optionally, the jaws can have a geometry which can also assist and guarantee homogenous distribution of the sample during the clamping and blocking stage.
According to a further embodiment, the system includes:
The roller or cylinder 22′ for support of the sample, together with the pair of lower rollers or cylinders 24a of the means for blocking/anchoring the sample, forms a first arrangement of elements 28a, whereas the roller 26 for tensioning or alignment of the sample, together with the pair of lower rollers or cylinders 24b, forms a second arrangement of elements 28b, which is at least partly interpenetrable with the first device 28a.
In
An exemplary, but non-limiting embodiment of the means 24 for blocking/anchoring the sample is illustrated in
According to a simpler embodiment, these jaws can have a parallelepiped cross-section, including with beveled edges.
In all cases, these elements or the surfaces in direct contact with the sample are preferably constructed of, or covered with, appropriate friction material which can guarantee an excellent hold, without causing any damage to the sample itself.
Optionally, but not necessarily, in order to be able to be subjected to analysis, the sample must be characterized by a certain parallelism of the elements which constitute it along their major axis. Sometimes this situation is already intrinsic in the sample (for example if a portion of eyelashes is taken into consideration, these can be approximately parallel to one another).
This characteristic need not be intrinsic in the sample itself (if account is taken for example of a lock of hairs which are curly or tousled). For this reason, the support element can optionally, but not necessarily, be coupled to an element which assists the tensioning and alignment of the elements which constitute the sample. Typically, but not necessarily, this element can comprise a rotary structure, such as, for example, the cylinder 26, shown in detail according to a lateral view and a view in perspective in
In order to generate a surface of the sample with characteristics suitable for the analysis, the surface of the tensioning/alignment element is characterized by a particular geometry (also determined by the cross-section of the prism), and can be constituted by, or coated with, a particular friction material which, by engaging on the sample, determines the stretching and optimum distribution thereof on the analysis surface. Typically, but not necessarily, the action of tensioning or alignment is carried out by means of the rotation of this element about its central axis (
By way of example, but not necessarily, this measurement can be made by means of measurement of the power absorbed by the gearmotor which actuates the tensioning/alignment element in order to start and keep it rotating.
If it is present, the tensioning/alignment element 26 is typically, but not necessarily, located on the side opposite the blocking/anchoring means 24, with the support element 22 interposed between the two. Its action contributes towards generating an appropriate analysis surface.
Alternative embodiments can be considered, which have the characteristics forming the subject of the present invention, for example for analysis of artificial samples (such as, for example, but not limited to, false eyelashes or synthetic fibers) or of samples obtained ex-vivo (such as, for example, but not limited to, eyelashes reconstituted using natural skin appendages such as animal hairs or other natural fibers, or locks of hair, etc.).
For use of this type, the system which is the subject of the invention need not necessarily be manageable manually, and optionally can thus be produced so as to be able to constitute a workbench apparatus which, although it is easy to transport, is used in a stationary manner.
In order to reduce the production costs and simplify the characteristics of use thereof, this embodiment can optionally be simplified. In particular, optionally, it would be possible to eliminate the tensioning or alignment element from the system. This simplified solution would be advantageous in the case of, for example, but not limited to, the study of samples of fibers (such as, for example, artificial or reconstituted eyelashes), the short length of which would make the tensioning/alignment element unnecessary.
In an example of application, the system according to the present invention was used to determine reference classes by means of image analysis, for example, but not necessarily, in order to classify samples of hairs on the basis of color. For this purpose, 4 groups were prepared constituted by 8 locks each, respectively of a light blonde color (BC), strawberry blonde (BR), light brown (CC) and medium brown (CM). By way of example,
In order to give an example of the effectiveness of application of the system which is the subject of the present invention in determining a modification of the optical response of a sample, for example for analysis of the effects of a treatment which worsens the characteristics of a hair, locks of hair as such (natural), and locks after reducing chemical treatment were analyzed. The results showed a radical change of the type of reflection/dispersion, as shown in
Analysis of the sample treated chemically and irradiated with various sources (green and red) gave results relating to the parameters, for example but not limited to, Scale, Chromatic and Contour, which can be summarized as a percentage of similarity (score) with respect to the reference (natural hair). Below, by way of example, the results are given of the aforementioned analysis, from which it is deduced that chemical treatment gives rise to variation of the score of more than 25%.
This variation is comparable with respect to the two sources used. These results confirm the capacity of the system according to the invention to show structural variations of the surface of the sample.
In order to give an example of the effectiveness of application of the system according to the invention in determining the effect of a treatment, for example, but not necessarily, a cosmetic treatment, analysis was carried out of locks of damaged hair and locks after cosmetic treatment with a conditioner. The table is given below, with the corresponding reprocessing of the samples irradiated with a red and green source.
The results show an improvement of the morphological characteristics of the sample which results in increase of the score of more than 15%. The results obtained on various locks, including those irradiated with various sources, were reproducible, and therefore confirm the reliability of the data produced by use of the invention.
The analysis of short filaments, such as, for example, but not necessarily, eyelashes, can be carried out with the system according to the invention, both in vivo and on models constructed ad hoc in order to evaluate the surface characteristics thereof which can be attributed to the reflection/dispersion images, as well as some specific parameters such as, for example, the thickness, the definition, the curvature and the linear density.
The image processing makes it possible to obtain a digital image profile, from which parameters of interest can be obtained, by applying appropriate algorithms.
The system according to the present invention was used to evaluate the performance levels of two different applicators for mascara in terms of homogeneity of distribution of the product. For this purpose, samples of a reconstituted eyelash model were prepared and divided into two groups (A and B). The same make-up product (mascara) was applied to each group, using two different applicators (A and B). The analyses were carried out in triplicate.
The first analysis carried out in order to evaluate the homogeneity of the eyelash samples prepared, the results of which are given in the following table, indicates that the samples do not differ from one another, and have a score of approximately 100%.
This result is fundamental for evaluating subsequently the effect of the use of the different applicators on samples which are different, but homogenous relative to one another.
The table contains for example the results obtained for the artificial eyelash samples, with evaluation of the Scale, the Chromatic and the Contour. The results show a substantial difference between the two samples, emphasizing the different performance levels of the two applicators. The similarity index (score) is reduced by almost 20%.
It will be appreciated that, whilst retaining the principle of the invention, the embodiments and details of implementation can be widely varied compared with those described and illustrated purely by way of non-limiting example, without however departing from the scope of the invention defined by the appended claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 102019000004861 | Apr 2019 | IT | national |
This application is a U.S. National Phase filing of PCT International Patent Application No. PCT/IB2020/053079, having an international filing date of Apr. 1, 2020, which claims priority to Italian Patent Application No. 102019000004861, filed Apr. 1, 2019 each of which is hereby incorporated by reference in its entirety.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/IB2020/053079 | 4/1/2020 | WO |
